Page 1
Operating instructions
CO2-Incubator
Heracell® 150i/240i
Heracell 150i/240i 50115191_C_01-2018 Thermo Scientific
Page 2
© 2018 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific provides this document to its customers with a product purchase to use in
the product operation. This document is copyright protected and any reproduction of the whole or
any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific. The contents of this document are subject to change without notice. Concerning translations into foreign languages, the English version of these operating instructions is binding.
All technical information in this document is for reference purposes only. System configurations and
specifications in this document is not part of any sales contract between Thermo Fisher Scientific
and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale,
which Terms and Conditions of Sale shall govern all conflicting information between the two documents
This regulation does not apply to reproductions for in-plant use.
Trademarks
All trademarks mentioned in this operating instructions are the exclusive property of the respective
manufacturers.
Thermo Fisher Scientific Inc.
168 3rd Avenue
Waltham, MA 02451
USA
.
Caution
When used as a medical device:
Federal law restricts this device to sale by or on the order of a qualified scientist with studies of
medicine, biology or chemistry.
Safety with regard to the protection of persons, the environment and the material under process
largely depends on the conduct of the persons using the equipment.
This operating manual is important for your safety as well as for the setup, installation, use and
maintenance of the equipment!
To avoid errors and any resulting damage, especially personal injuries, be sure to read this manual carefully before using the equipment and follow all instructions.
Heracell 150i/240i 50115191_C_01-2018 Thermo Scientific
Page 3
Table of Contents
Chapter 1 General notes................................................................................................ 9
Overview of the Thermo Fisher international sales
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . organizations9
Identification of the device and of the documentation . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Instruction of the operating personnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Applicability of the instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Explanation of safety information and symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Intended purpose of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Standards and directives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Safety notes on gases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Operational Safety Rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 2 Delivery of the device.................................................................................. 19
Packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Acceptance inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Standard equipment components HERACELL® 150i. . . . . . . . . . . . . . . . . . . . . . . . . . 20
Optional equipment components HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Standard equipment components HERACELL® 240i. . . . . . . . . . . . . . . . . . . . . . . . . . 21
Optional equipment components HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Chapter 3 Installation of the device ............................................................................. 23
Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Room ventilation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Stacking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Intermediate Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Retrofitting/Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Chapter 4 Description of the device............................................................................. 29
Front view of HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Rear view HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Front view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Rear view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Safety devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Work space atmosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Sensor system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Supply interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Work space components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Chapter 5 Start-up ...................................................................................................... 57
Let the device adapt to the ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Preparing the work space . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Installing the shelf system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Installing the gas humidification
(optional, only for O2/N2-control) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Installing the optional bottle turning devices HERACELL® 240i . . . . . . . . . . . . . . . . . . 62
Installing the optional center strut HERACELL® 240i for split inserts . . . . . . . . . . . . . . 64
Gas connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Heracell 150i/240i 50115191_C_01-2018 3 Thermo Scientific
Page 4
Power supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Connecting the RS 232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Connecting the USB interface (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Connecting the alarm contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Chapter 6 Operation.................................................................................................... 75
Prior to starting operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Preparing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Starting operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Interruption of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Chapter 7 .......................................................Handling and control (iCanTM touchscreen
controller) ......................................................................................................................... 79
Power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Operating panel and operating structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Versions without O2/N2 control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Version with combined CO2/O2/N2 control (optional) . . . . . . . . . . . . . . . . . . . . . . . . . 82
Structure of the operating levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Factory presettings of the iCanTM touchscreen controls . . . . . . . . . . . . . . . . . . . . . . . 84
Heat-up phase of the control loop sensors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Event actions for settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Setting the temperature set value
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Setting the CO2 set value. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Setting the O2 set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Auto-start routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Running the contra-con routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
User configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Scaling the trend display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Chapter 8 Shut-down................................................................................................ 132
Shutting the device down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
Chapter 9 Cleaning and disinfection.......................................................................... 133
Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Decontamination procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Wipe/spray disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
contra-con decontamination routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Chapter 10 Maintenance .......................................................................................... 145
Inspections and checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Service intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Returns for Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Preparing the temperature calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Temperature calibration procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Preparing the CO2 calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
CO2 calibration procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
Replacing the gas inlet filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Replacing the device fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
Replacing the door seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Chapter 11 Disposal ................................................................................................. 159
Heracell 150i/240i 50115191_C_01-2018 4 Thermo Scientific
Page 5
Chapter 12 Technical data........................................................................................ 161
HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
Chapter 13 Data communication .............................................................................. 165
Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Structure of the command sequences for data
communication. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
Overview of general parameters (addresses 0xxx) . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Overview of incubator parameters (addresses 2xxx) . . . . . . . . . . . . . . . . . . . . . . . . . . 170
Error memory structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
Data logger structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Examples of data logger codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Program HERACELL® 150i / 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Chapter 14 Device log .............................................................................................. 193
Heracell 150i/240i 50115191_C_01-2018 5 Thermo Scientific
Page 6
Figures
Figure 15. Device dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Figure 16. Lift points . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Figure 17. Stacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Figure 18. Front view HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Figure 19. Rear view HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Figure 20. Looped-through gas supply HERACELL® 150i . . . . . . . . . . . . . . . . . . . . . . . 32
Figure 21. Front view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Figure 22. Rear view HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Figure 23. Looped-through gas supply HERACELL® 240i . . . . . . . . . . . . . . . . . . . . . . . 36
Figure 24. Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Figure 25. Temperature, CO2 and O2 sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Figure 26. Supply interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Figure 27. Work space components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Figure 28. Water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Figure 29. Rear panel openings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Figure 30. Bottle turning device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Figure 31. Water pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Figure 32. Support rail installation/removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Figure 33. Shelf support installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Figure 34. Installing gas humidification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Figure 35. Installing the bottle turning devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Figure 36. Bottle turning speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Figure 37. Center strut installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Figure 38. Installing gas pressure hoses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Figure 39. Gas connection without gas monitoring system . . . . . . . . . . . . . . . . . . . . . . . 66
Figure 40. Gas connection with gas monitoring system (optional) . . . . . . . . . . . . . . . . . . 67
Figure 41. Combined connection with gas monitoring system (optional) . . . . . . . . . . . . . 68
Figure 42. Power supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Figure 43. Connection example . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Figure 44. Power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Figure 45. Pressure-sensitive areas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Figure 46. iCanTM touchscreen without O2/N2 gas supply . . . . . . . . . . . . . . . . . . . . . . . 81
Figure 47. iCanTM touchscreen with combined gas connection . . . . . . . . . . . . . . . . . . . 82
Figure 48. Menu structure overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Figure 49. Heat-up phase display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Figure 50. Setting the temperature set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Figure 51. Setting the CO2 set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Figure 52. Setting the O2 set value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Figure 53. Activating the auto-start routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Figure 54. Interrupting the auto-start routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Figure 55. Changing the code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Figure 56. Date / time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Figure 57. Display contrast setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Figure 58. Key tone setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Figure 59. RS 232 interface baud rate setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Figure 60. USB interface baud rate setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Figure 61. Language setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Figure 62. Reminder interval setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Figure 63. Event display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Figure 64. Logging cycle setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Figure 65. Error table display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Figure 66. Alarm relay setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Figure 67. Low humidity setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Figure 68. Gas tight screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Heracell 150i/240i 50115191_C_01-2018 6 Thermo Scientific
Page 7
Figure 69. Water level setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Figure 70. Alarm relay setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Figure 71. Bottle turning device speed setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Figure 72. Switching the O2 control on and off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Figure 73. Icon description overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Figure 74. Gas monitoring icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Figure 75. Enabling/disabling the keypad lock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Figure 76. Software versions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Figure 77. Trend display scaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Figure 78. Event error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Figure 79. Event error overtemperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
Figure 80. Decontamination routine phases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Figure 81. Activating contra-con . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
Figure 82. Interrupting / canceling contra-con . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
Figure 83. contra-con completion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Figure 84. Preparing the temperature calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
Figure 85. Temperature calibration procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Figure 86. Preparing CO2 calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Figure 87. Gas-tight screen measurement opening . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
Figure 88. CO2 calibration procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
Figure 89. Gas inlet filter installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
Figure 90. Device fuse replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Figure 91. Magnetic door seal replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
Heracell 150i/240i 50115191_C_01-2018 7 Thermo Scientific
Page 8
Heracell 150i/240i 50115191_C_01-2018 8 Thermo Scientific
Page 9
1. General notes
1.1 Overview of the Thermo Fisher international sales organizations
Postal address Germany:
Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Enquiries from Germany:
Phone Sales 0800 1 536376
Phone Service 0800 1 112110
Fax Sales/Service 0800 1 112114
E-Mail info.labequipment.de@thermofisher.com
Enquiries from Europe, Middle East, and Africa:
Phone + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-7474
E-Mail info.labequipment.de@thermofisher.com
Postal address USA:
Thermo Fisher Scientific Inc.
168 3rd Avenue
Waltham, MA 02451
USA
Enquiries from North America:
Phone +1 800-879 7767
Fax +1 828-658 0363
E-Mail info.labequipment@thermofisher.com
Enquiries from Latin America:
Phone +1 828-658 2711
Fax +1 828-645 9466
E-Mail info.labequipment@thermofisher.com
Enquiries from Asia Pacific:
Phone +852-2711 3910
Fax +852-2711 3858
E-Mail info.labequipment@thermofisher.com
Heracell 150i/240i 50115191_C_01-2018 9 Thermo Scientific
Page 10
1.2 Identification of the device and of the documentation
Identification of the device
Device name: CO2 incubator
Type designation: HERACELL
HERACELL
®
150i
®
240i
Serial No.: 40830469 or higher
Allocation of product documentation
User information: Operating instructions 50115191
Valid: 01.2018
Certifications and quality audit:
Certification: CE Certification
Test mark: GS by VDE Germany
CSA by VDE Germany
1.3 Instruction of the operating personnel
These operating instructions describe the CO2 incubator HERACELL
has been manufactured in keeping with the latest technological developments and is operationally
safe. However, the device may present potential hazards, particularly if it is operated by
inadequately trained personnel or if it is not used in accordance with the intended purpose.
Therefore, the following must be observed to prevent accidents:
®
150i/240i. The CO
incubator
2
•The CO2 incubator must be operated only by trained and authorized personnel.
• For any operation of this device, the operator must prepare clear and concise written
instructions in the language of the operating and cleaning personnel based on these operating
instructions, applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular:
which decontamination measures are to be applied for the CO2 incubator and accessories,
which protective measures apply when specific agents are used,
which measures are to be taken in the case of an accident.
• Repairs to the device must be carried out only by trained and authorized expert personnel.
1.4 Applicability of the instructions
• The contents of the operating instructions are subject to change without further notice.
• Concerning translations into foreign languages, the German version of these operating
instructions is binding.
• Keep these operating instructions close to the device so that safety instructions and important
information are always accessible.
Should you encounter problems that are not detailed adequately in these operating instructions,
please contact Thermo Scientific immediately for your own safety.
Heracell 150i/240i 50115191_C_01-2018 10 Thermo Scientific
Page 11
1.5 Warranty
Thermo Scientific warrants the operational safety and functions of the CO2 incubator only under
the condition that:
• the device is operated and serviced exclusively in accordance with its intended purpose and as
described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Thermo Fisher Scientific
are used,
• inspections and maintenance are performed at the specified intervals.
The warranty is valid from the date of delivery of the device to the operator.
1.6 Explanation of safety information and symbols
Safety information and symbols used in the operating instructions
WARNUNG
Indicates a hazardous situation which, if not avoided, could result in death or serious injury!
Caution
Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries.
ACHTUNG
Indicates a situation which, if not avoided, could result in property damage.
Hinweis
Is used for applicational hints and useful information.
Additional symbols for safety informations:
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Heracell 150i/240i 50115191_C_01-2018 11 Thermo Scientific
Page 12
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
Symbols on the device
CE conformity mark: confirms conformity according to EU Guidelines
VDE test mark
Mark of conformity USA/Canada
Observe operating instructions!
Mark of prescription devices!
Heracell 150i/240i 50115191_C_01-2018 12 Thermo Scientific
Page 13
1.7 Intended purpose of the device
Correct use
Correct use related to a medical device (USA only)
Caution
When used as a medical device:
Federal law restricts this device to sale by or on the order of a qualified scientist with studies of
medicine, biology or chemistry.
The intended use of this incubator is to provide an environment with
• controlled temperature,
•CO2,
• elevated humidity,
• an automatic decontamination mode, and
•O2 at suppressed levels,
for the development of ova or embryos at or near body temperature.
Correct use related to a laboratory device (Rest of the world)
The CO
incubator HERACELL® 150i/240i is a laboratory device for preparing and cultivating cell
2
and tissue cultures. The device allows the simulation of the special physiological ambient
conditions for these cultures due to the exact control of:
• temperature,
•CO
•O2/N
content,
2
content,
2
• and increased relative humidity.
Fields of Application
HERACELL® 150i/240i has been designed for installation and operation in the following fields of
application:
• Laboratories for cytobiological and biotechnological experiments of safety levels L1, L2, and L3
• Medical-microbiological laboratories in accordance with DIN EN 12128
• Laboratories in the central area of clinics and hospitals
The gases required for the incubator (CO2 and / or O2/N2) are supplied to the device from a
separate gas supply system, either from gas cylinders or from a central pressurized gas container.
Heracell 150i/240i 50115191_C_01-2018 13 Thermo Scientific
Page 14
The layout of the gas supply system must ensure that the operating pressure of the gas supply
lines can be set to a range between 0.8 bar (min.) to 1 bar (max.) and that the pressure cannot be
changed.
If the device is equipped with the optional gas monitoring system, up to four devices can be
interconnected in line, independent of the of the gas supply system capacity.
The CO
incubator is suitable for continuous operation.
2
Incorrect use
Do not use cell or tissue cultures in the device that are not in accordance with the regulations of
safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:
• are easily ignitable or explosive,
• release vapors that form combustible or explosive mixtures when exposed to air,
• release poisons,
• release dust,
• exhibit exothermic reactions,
• are pyrotechnical substances.
1.8 Standards and directives
The device complies with the following standards and guidelines:
• DIN EN 61010 - 1:2011, DIN EN 61010 - 2 - 010:2015
• Low Voltage Guideline 2014/35/EU
• EMC Guideline 2014/30/EU
• China EEP hazardous substance informations
http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html
The following safety regulations must be observed if the device is operated within the territory of
the Federal Republic of Germany:
• DGUV Guideline 100-500
• TRBS 3145/TRGS 725
• Safety data sheets of the gas suppliers with regard to the particular characteristics of CO2, O2,
and N2.
• Principles of good microbiological proceedings, notice of the trade association of the German
chemical industry.
For other countries, the applicable national regulations are binding.
Heracell 150i/240i 50115191_C_01-2018 14 Thermo Scientific
Page 15
1.9 Safety notes
• In the case of biological incubators, biological safety with regard to the protection of
persons, its surroundings and the load is heavily dependent on the observance of
the applicable regulations by the persons using the equipment.
• Even then, however, the possibility of hazards, especially health hazards, arising
cannot be ruled out.
• The residual risk depends on the work performed in each individual case.
• To avoid errors and causing damage, especially personal injuries, be sure to
carefully read this manual before putting the equipment into operation, and follow
all instructions.
• Safe and reliable operation of the unit can only be guaranteed if the necessary
inspections, maintenance and repair work are carried out by Thermo Electron LED
GmbH Service Department personnel or by personnel authorized by our company.
• Gas is to be supplied to each unit by means of a pressure reducer with the inlet
pressure set to between 0.8 and max. 1 bar. This setting must not be changed for
safety reasons.
• The place of installation must be thoroughly ventilated in order to expel the gases
escaping around the pressure relief valve to the outside.
• Only original replacement parts authorized by the manufacturer are to be used.
• Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL
ENGINEERINGPRACTICE
Heracell 150i/240i 50115191_C_01-2018 15 Thermo Scientific
Page 16
1.10 Safety notes on gases
Hinweis
Installation work:
Any work to supply lines and pressurized gas containers, cylinders or containers used for storing CO2 or
O2/N2 must only be carried out by expert personnel using the appropriate tools.
Instruction of the personnel:
Personnel operating devices with CO2 supply must be instructed about the particularities in the handling of
CO2 before starting their work:
• correct operation of pressurized gas containers and gas supply systems,
• obligation to report damages and shortcomings in CO2 supply lines,
• measures to be taken in case of accidents or failures.
These instructions must be repeated at appropriate intervals and must comprise the particular operating
instructions of the gas supplier.
Safety notes on carbon dioxide (CO2)
Since CO2 is rated as a harmful gas, certain safety instructions must be observed when the CO2
incubator is started up and when the device is operated.
Suffocation hazard!
Large amounts of CO2 released into the room atmosphere may cause suffocation.
If CO2 is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to enter the room!
• Inform security service or fire department!
Safety notes on oxygen (O2)
O2 is a gas that promotes combustion and may explode in combination with grease-containing
materials.
Oxygen explosion!
O2 may explode in combination with oils, greases, and lubricants. If highly compressed oxygen
comes in contact with grease- or oil-containing substances, the mixture may explode!
• For cleaning these device components, use only oil- and grease-free lubricants.
• Keep all connections and components of the oxygen system free from substances that
contain oil, grease, or lubricant!
Heracell 150i/240i 50115191_C_01-2018 16 Thermo Scientific
Page 17
Fire hazard!
Released oxygen promotes combustion. Do not use open flames in the vicinity of
oxygen-operated systems!
• Do not smoke in the vicinity of oxygen systems.
• Do not expose the components of an oxygen system to excessive heat.
Safety notes on nitrogen (N2)
Nitrogen mixes easily with air. High concentrations of nitrogen reduce the oxygen content in the air.
Suffocation hazard!
Large amounts of N2 released into the room atmosphere may cause suffocation.
If N2 is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to enter the room!
• Inform security service or fire department!
1.11 Operational Safety Rules
The following rules must be heeded when working with Heracell 150i/240i:
• Observe the sample weight limits specified for your Heracell-incubator as a whole and its
shelving in particular (Seite 161).
• Arrange the samples evenly throughout the work space, making sure not to place them too
closely to the interior walls to ensure a uniform temperature distribution.
• Do not load your CO2-incubator with substances that exceed the capabilities of the available lab
apparatus and Personal Protection Equipment to provide sufficient degrees of protection to
users and third parties (Seite 14).
• Check the door seal once a month for proper sealing performance and possible damage.
• Do not process any samples containing hazardous chemical substances that may be released
into the ambient air through defective seals or may cause corrosion or other defects on parts of
the CO2-incubator.
• The tempering of defined substances or materials with higher moisture content can result in
increased condensation in the chamber. Measures on page 77 must be observed.
Heracell 150i/240i 50115191_C_01-2018 17 Thermo Scientific
Page 18
Heracell 150i/240i 50115191_C_01-2018 18 Thermo Scientific
Page 19
Chapter 2 Delivery of the device
2. Delivery of the device
2.1 Packaging
The CO2 incubator HERACELL
materials can be separated and are reusable:
• Packaging carton: Recycled paper
• Foam elements: Styrofoam (CFC-free)
• Pallet: Untreated wood
• Packaging film: Polyethylene
• Packaging ribbons: Polypropylene
®
150i/240i is delivered in a stable packaging box. All packaging
2.2 Acceptance inspection
After the device has been delivered, check the delivery immediately for:
• completeness
• possible damage
If components are missing or damage is found on the device or the packaging, esp. damages caused by
humidity and water, please contact the line-hauler as well as the Technical Support of Thermo Scientific
immediately.
Caution
Risk of injury
Should sharp edges have formed in damaged areas or elsewhere on the device, take all
necessary precautions to protect personnel handling the incubator. For example, have them
wear protective gloves and other personal protection equipment.
Heracell 150i/240i 50115191_C_01-2018 19 Thermo Scientific
Page 20
Chapter 2 Delivery of the device
2.3 Standard equipment components HERACELL® 150i
®
150i
3-door gas tight screen and
continuous shelves
Quantity of the delivered components (in piece)
HERACELL
CO
incubator or CO2 /O2 incubator
2
Solid glass door and
continuous shelves
Shelf 3 3
Support rail for shelf 4 4
Shelf supports for shelf 6 6
Insert for pressure compensation opening 1 1
Plug for pipe channel 1 1
Power supply cable 1 1
Connector, potential-free contact 1 1
Spare caps, set 1 1
CO
connecting hose set 1 1
2
Open-end wrench, 24 mm 1 1
Allen wrench 2 mm for blower wheel 1 1
Allen wrench 3 mm for blower wheel cover 1 1
Operating instructions (CD) 1 1
Summarized safety instructions
Water pump 1 1
2.4 Optional equipment components HERACELL® 150i
HERACELL
CO
incubator or CO2 /O2 incubator
Quantity of the delivered components (in piece)
2
Solid glass door and
continuous shelves
O
connecting hose set 1 1
2
sensor with set for gas humidification *1) 1 1
O
2
connecting hose set, gas monitoring (optional) 1 1
CO
2
/ N2 connecting hose set, gas monitoring
O
2
11
(optional)
*1) O2 sensor with gas humidification set is packed in a separate carton and placed into the work space during transport.
®
150i
3-door gas tight screen and
continuous shelves
Heracell 150i/240i 50115191_C_01-2018 20 Thermo Scientific
Page 21
Chapter 2 Delivery of the device
2.5 Standard equipment components HERACELL® 240i
HERACELL
CO
incubator or CO2 /O2 incubator
2
Quantity of the delivered
components (in piece)
Shelf 3 6 3 6
Support rail for shelf 4 6 4 6
Shelf supports for shelf 6 12 6 12
Insert for pressure
compensation opening
Plug for pipe channel 1 1 1 1
Power supply cable 1 1 1 1
Connector, potential-free contact
Spare caps, set 1 1 1 1
CO
connecting hose
2
set
Open-end wrench,
24 mm
Allen wrench 2 mm for
blower wheel
Allen wrench 3 mm for
blower wheel cover
Operating instructions
(CD)
Summarized safety
instructions
Water pump 1 1 1 1
Solid glass door
and continuous
shelves (standard
version)
11 1 1
11 1 1
11 1 1
11 1 1
11 1 1
11 1 1
11 1 1
Solid glass door
and split shelves
(optional)
®
240i
6-door gas tight
screen and
continuous shelves
(standard version)
6-door gas tight
screen and split
shelves (optional)
®
2.6 Optional equipment components HERACELL
HERACELL
CO
incubator or CO2 /O2 incubator
2
Quantity of the delivered
components (in piece)
O
connecting hose
2
set
Heracell 150i/240i 50115191_C_01-2018 21 Thermo Scientific
Solid glass door
and continuous
shelves (standard
version)
11 1 1
Solid glass door
and split shelves
(optional)
®
240i
6-door gas tight
screen and
continuous shelves
(standard version)
240i
6-door gas tight
screen and split
shelves (optional)
Page 22
Chapter 2 Delivery of the device
O2 gas humidification
11 1 1
system connecting
hose set *
Bottle turning device
1
)
1- 1 (motor with four roll-
ers, optional) *
CO
connecting hose
2
2
), *3)
11 1 1
set, gas monitoring
(optional)
O
connecting hose
2/N2
11 1 1
set, gas monitoring
(optional)
*1) O2 sensor with gas humidification set is packed in a separate box that is placed into the work space during transport.
*2) For bottle turning devices, the version with split shelves is not available.
*3) Each level of a bottle turning device is packed in a separate box. The boxes (1 to 4) are delivered with the device.
Heracell 150i/240i 50115191_C_01-2018 22 Thermo Scientific
Page 23
Chapter 3 Installation of the device
3. Installation of the device
3.1 Ambient conditions
The device must only be operated at locations that meet the particular ambient conditions listed
below:
• Draft-free and dry location.
• The minimal distance to adjacent surfaces must be observed on all sides (see „Space
requirements“ on page 24).
• The operating room must be equipped with appropriate room ventilation.
• Solid, level, fire-proof surface.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the weight of the device
and of accessories (particularly if several devices are stacked).
Caution
Contamination hazard
Do not place the incubator directly on the lab floor, but mount it on the floor stand or on a lab
work surface (option; to be ordered separately). Contaminants, such as bacteria, viruses, fungi,
prions, and other biological substances may use the open door to migrate easily from the floor
into the incubator’s work space.
• The electrical system of the device has been designed for an operating height of up to 2000 m
above sea level.
• To ensure a constant incubation temperature of 37 °C, the ambient temperature must be within
a range of +18 °C to +33 °C.
Notice
To high or to low ambient temperatures
If the ambient temperatures are to high or to low, the properly functionality of the incubator
could be faulty.The incubation temperature as well as the temperature distribution could be
false or affected.
• Relative humidity up to 80 % (max.).
• No direct exposure to sunlight.
• Devices that produce excessive heat must not be placed near the location of the HERACELL
150i/240i.
• If excessive temperatures tend to occur in the operating room, be sure to provide a thermal
protection means that cuts out the power supply to mitigate the impact of over-temperature
scenarios.
• Power line voltage variations must not exceed ±10 % of the nominal voltage.
• Transient surges must lie within the range of levels that normally occur in the power supply
Heracell 150i/240i 50115191_C_01-2018 23 Thermo Scientific
®
Page 24
Chapter 3 Installation of the device
system. The impulse withstand voltage based on surge category II of IEC 60364-4-444 shall be
applied at the nominal voltage level
• Do not cover or obstruct the fresh air or exhaust vents in the unit housing.
• Avoid condensation, e.g. after transport or change of location. Should condensation exist, wait
until the moisture has evaporated completely before connecting the CO2-incubator to a power
source and powering up.
Notice
Humidity
After transport or storing under humid conditions a drying-out process must be performed.
During the drying-out process the equipment cannot be assumed to meet all the safety
requirements of the IEC 61010-2-010 standard. The drying-out period is 2 hours.
• Consider installing a dedicated upstream circuit breaker per Incubator to avoid the failure of
multiple devices in case of an electrical fault.
3.2 Room ventilation
When CO2/O2/N2 is supplied, the work space of the incubator is slightly pressurized. The pressure
is released through the pressure compensation opening into the operating room.
Pressure compensation and any opening of the glass door/gas-tight screen during the operation of
the device will release very small quantities of CO2/O2/N2 into the operating room; the room
ventilation must be capable of carrying the released gas safely off into the open.
In addition, heat dissipating from the device during continuous operation may cause a change in
the room climate.
• Therefore, the HERACELL
• When several devices are to be placed in the same room, additional ventilation may have to be
provided as necessary.
• Do not install the device in room recesses without ventilation.
• The room ventilation should be a technical ventilation that complies with the requirements of
DGVU information 213-850 (Guidelines for laboratories, Germany) or some other suited
ventilation system with appropriate capacity.
®
150i/240i must only be installed in rooms with sufficient ventilation.
3.3 Space requirements
Noteice
Make sure that power sockets remain freely accessible at all times!
To allow a rapid disconnection of power in case of an emergency, make sure that power
outlets remain freely accessible at all times!
When installing the device, make sure that the installation and supply connections are freely
accessible.
The control box on the rear panel of the device may serve as a spacer to adjacent objects. The
Heracell 150i/240i 50115191_C_01-2018 24 Thermo Scientific
Page 25
Chapter 3 Installation of the device
side distances given are minimal distances.
To protect the CO2 incubator against contamination, use a floor stand even if the device is installed
near the floor. The height of the floor stand should not fall below 200 mm.
Figure. 1. Device dimensions
Note
Accessibility of the devices:
To ensure the accessibility for care and maintenance works, keep larger side and rear distances.
Heracell 150i/240i 50115191_C_01-2018 25 Thermo Scientific
Page 26
Chapter 3 Installation of the device
3.4 Transport
For transport, do not lift the device using the doors or components attached to the device (e.g.
control box on rear panel) as lift points.
Figure. 2. Lift points
Caution
Heavy loads! Lift with care!
To avoid injury through physical strain, such as strain traumata and slipped discs, do not
attempt to lift the incubator alone!
To avoid injury through dropped loads, be sure to wear Personal Protection Equipment, such as
safety shoes, when lifting the incubator. To avoid crushing your fingers or hands (particularly in
a closing door) or damaging the incubator, do not use any other lift points than those indicated
in the illustration above.
Note
Lift points:
Lift the device only using the lift points.
Notice
Humidity
After transport or storing under humid conditions a drying-out process must be performed.
During the drying-out process the equipment cannot be assumed to meet all the safety
requirements of the IEC 61010-2-010 standard. The drying-out period is 2 hours.
Heracell 150i/240i 50115191_C_01-2018 26 Thermo Scientific
Page 27
Chapter 3 Installation of the device
3.5 Stacking
HERACELL
®
150i/240i is suited for stacking up to two devices of the same device type.
HERAcell 240i only: For thermal separation, an adapter plate [3] can be placed between the two
devices.
HERACELL
®
150i
• Place the device to be stacked plate with the device stands [1] onto the stacking elements [5]
on top of the device. Make sure that the device stands are seated safely on the stacking
elements.
HERACELL
®
240i
• Place the adapter plate with the receptacle recesses [8] at the bottom onto the stacking
elements [9] on top of the device. Make sure that the adapter plate is seated safely on the
recesses.
• Place the device to be stacked with the device stands [6] onto the stacking elements [7] at the
top of the adapter plate.
The devices are secured to one another by their own weight.
Figure. 3. Stacking
Notice
Transporting stacked devices!
The stacking elements are not locking elements. Therefore, it is not permitted to transport stacked
devices.
Heracell 150i/240i 50115191_C_01-2018 27 Thermo Scientific
Page 28
Chapter 3 Installation of the device
Note
Fastening on mobile racks:
If the devices are installed onto mobile racks, make sure that the rollers [10] are secured with a locking
brake during the operation of the incubators and that the rollers are oriented toward the front for increased
stability.
Condensate formation during the operation of stacked devices:
If stacked devices of type HERACELL® 150i are operated at an ambient temperature of more than 26 °C,
condensate formation may occur on the upper device while the contra-con decontamination routine is run
for the lower device.
3.6 Intermediate Storage
When the oven is placed in intermediate storage, which is permissible for a maximum of four
weeks, make sure that the ambient temperature is between 20 °C to 60 °C (68 °F to 140 °F) and
the maximum relative humidity does not exceed 90 %, non-condensing.
3.7 Retrofitting/Modifications
The following components can be retrofitted to the standard version:
HERACELL
• 3-segment gas-tight screen (the gas-tight screen is installed in place of the glass door)
• door hinge replacement on outer door and glass door (RH / LH door opening)
• lockable outer door
• split inserts
• infrared (IR) sensor
• PCB for USB interface
HERACELL
• 6-segment gas-tight screen (the gas-tight screen is installed in place of the glass door)
• door hinge replacement on outer door and glass door
• lockable outer door
• split inserts
®
150i
®
240i
• infrared (IR) sensor
• PCB for USB interface
Note
Modifications:
Retrofitting and modifications must only be performed by the Technical Service of Thermo Electron LED
GmbH.
Heracell 150i/240i 50115191_C_01-2018 28 Thermo Scientific
Page 29
Chapter 4 Description of the device
4. Description of the device
4.1. Front view of HERACELL
®
150i
Figure. 4. Front view HERACELL
1. Stacking elements
2. Plug caps
3. Glass door
4. Measuring cell with blower wheel and sensors
5. Door switch
6. Oxygen sensor (optional)
7. Pressure compensation opening with insert
8. Measuring opening
Heracell 150i/240i 50115191_C_01-2018 29 Thermo Scientific
®
150i
Page 30
9. Outer door
10. Outer door seal, replaceable
11. Stand, height-adjustable
12. Gas humidification (optional)
13. Water level sensor
14. Nameplate
15. Power switch
16. Support rail
17. Shelf
18. Latch, glass door
19. Support hook for shelf
20. Access port with plug
21. Glass door seal, replaceable
22. Operating panel (touchscreen)
23. Door handle
Chapter 4 Description of the device
4.2. Rear view HERACELL® 150i
Figure. 5. Rear view HERACELL
Heracell 150i/240i 50115191_C_01-2018 30 Thermo Scientific
®
150i
Page 31
Chapter 4 Description of the device
1. Access port, Ø 42 mm
2. Pressure compensation opening
3. Control box with supply interface for combined gas connection (optional) CO2 and O2/N2 without
gas monitoring
4. Condensate drain gutter
5. Diagram: Gas connection CO2 and O2/N2 without gas monitoring
6. O2/N2 gas cylinder
7. CO2 gas cylinder
8. Secondary CO2 gas supply B with optional gas monitoring
9. Primary CO2 gas supply A with optional gas monitoring
10. Secondary O2/N2 gas supply B with optional gas monitoring
11. Primary O
12. Control box with supply interface for combined gas connection (optional) CO
gas supply A with optional gas monitoring
2/N2
and O2/N2 with gas
2
monitoring (optional)
13. Distributor connection for looping through the CO2 gas supply for up to three additional devices
(independent of device type)
14. Distributor connection for looping through the O2/N2 gas supply for up to 3 additional devices
(independent of device type)
15. Diagram: Gas connection CO2 and O2/N2 with optional gas monitoring
Heracell 150i/240i 50115191_C_01-2018 31 Thermo Scientific
Page 32
Chapter 4 Description of the device
Looped-through gas supply for HERACELL
®
150i:
Figure. 6. Looped-through gas supply HERACELL
®
150i
1. Control box with supply interface for combined gas connection (optional) CO2 and O2/N2 with
gas monitoring (optional)
2. Distributor connection for looping through the CO2 gas supply for up to three additional
devices (independent of device type)
3. Distributor connection for looping through the O2/N2 gas supply for up to 3 additional devices
(independent of device type)
4. Tee joint for fitting the gas pressure hoses together
5. Gas pressure hose for looping-through the gas supply
6. Diagram: Gas connection CO2 and O2/N2 with optional gas monitoring
7. HERACELL
combined gas connection (optional) CO2 and O2/N
Heracell 150i/240i 50115191_C_01-2018 32 Thermo Scientific
®
150i with looped-through gas supply; therefore device must only be equipped with
2
Page 33
Chapter 4 Description of the device
4.3. Front view HERACELL® 240i
Figure. 7. Front view HERACELL
®
240i
1. Stacking elements
2. Plug caps
3. Glass door
4. Measuring cell with blower wheel and sensors
5. Door switch
6. Oxygen sensor (optional)
7. Receptacle for bottle turning device (optional)
8. Measuring opening
9. Plug for bottle turning device (optional)
10. Outer door
Heracell 150i/240i 50115191_C_01-2018 33 Thermo Scientific
Page 34
11. Outer door seal, replaceable
12. Stand, height-adjustable
13. Drive roller for bottle turning device (optional)
14. Gas humidification (optional)
15. Water level sensor
16. Insert for bottle turning device (optional)
17. Nameplate
18. Cylinders (optional)
19. Power switch
20. Support rail
21. Shelf
22. Latch, glass door
23. Support hook for shelf
24. Pressure compensation opening with insert
25. Access port with plug
26. Glass door seal, replaceable
Chapter 4 Description of the device
27. Operating panel (touchscreen)
28. Door handle
Heracell 150i/240i 50115191_C_01-2018 34 Thermo Scientific
Page 35
Chapter 4 Description of the device
4.4. Rear view HERACELL® 240i
Figure. 8. Rear view HERACELL
®
240i
1. Pressure compensation opening
2. Access port, Ø 42 mm
3. Condensate drain gutter
4. Control box with supply interface for combined gas connection (optional) CO
and O2/N2
2
without gas monitoring
5. Diagram: Gas connection CO2 and O2/N2 without gas monitoring
6. O2/N2 gas cylinder
7. CO2 gas cylinder
8. Secondary CO2 gas supply B with optional gas monitoring
Heracell 150i/240i 50115191_C_01-2018 35 Thermo Scientific
Page 36
Chapter 4 Description of the device
9. Primary CO2 gas supply A with optional gas monitoring
10. Secondary O2/N2 gas supply B with optional gas monitoring
11. Primary O2/N2 gas supply A with optional gas monitoring
12. Control box with supply interface for combined gas connection (optional) CO2 and O2/N2 with
gas monitoring (optional)
13. Distributor connection for looping through the CO2 gas supply for up to three additional
devices (independent of device type)
14. Distributor connection for looping through the O2/N2 gas supply for up to 3 additional devices
(independent of device type)
15. Diagram: Gas connection CO2 and O2/N2 with optional gas monitoring
Looped-through gas supply for HERACELL
®
240i:
Figure. 9. Looped-through gas supply HERACELL
Heracell 150i/240i 50115191_C_01-2018 36 Thermo Scientific
®
240i
Page 37
Chapter 4 Description of the device
1. Control box with supply interface for combined gas connection (optional) CO2 and O2/N2 with
gas monitoring (optional)
2. Distributor connection for looping through the CO2 gas supply for up to three additional
devices (independent of device type)
3. Distributor connection for looping through the O2/N2 gas supply for up to 3 additional devices
(independent of device type)
4. Tee joint for fitting the gas pressure hoses together
5. Gas pressure hose for looping-through the gas supply
6. Diagram: Gas connection CO2 and O2/N2 with optional gas monitoring
7. HERACELL
combined gas connection (optional) CO2 and O2/N
®
240i with looped-through gas supply; therefore device must only be equipped with
2
4.5. Safety devices
The device is equipped with the following safety devices:
• A door switch interrupts the CO2/O2/N2 supply and the work space heating when the glass
door is opened.
• The optional gas detector switches the gas supply over to a full gas cylinder.
• An independent thermal protection protects the samples from harmful overheating in case of
failures.
• A pressure compensation opening ensures pressure compensation in the device work space.
• The alarm relay circuit uses audible and visual alarms to indicate errors during operation.
4.6. Work space atmosphere
In the work space of the incubator, the particular physiological ambient conditions for the
preparation and cultivation of cell and tissue cultures are simulated. The work space atmosphere is
determined by the following factors:
• Temperature
• Relative humidity
•CO2 concentration
•O2 concentration (optional)
Temperature:
To ensure undisturbed operation, the temperature in the operating room must be at least 18 °C
and the incubation temperature must exceed this temperature by at least 3 °C.
The heating system controls the incubation temperature from this temperature threshold up to
55 °C. The principle of air jacket heating and the additional, separate heating of the outer door and
glass door/gas-tight screen minimize the risk of condensate formation at the side walls, at the
ceiling of the work space, and at the glass door/gas-tight screen.
Heracell 150i/240i 50115191_C_01-2018 37 Thermo Scientific
Page 38
Chapter 4 Description of the device
Relative humidity:
The heating of the work space promotes the condensation of the water, thereby ensuring a
constant humidity within the work space. For the running operation, always keep a sufficient
quantity of processed water of the following quality available:
• HERACELL
• HERACELL
®
240i: quantity 4.5 l
®
150i: quantity 3.0 l
Water quality recommendation:
For trouble-free operation of the equipment, fill the water reservoir with sterilized distilled water or
equivalent. The acceptable conductivity should be within the range of 1 to 20 S (resistivity within
the range of 50 k to 1 M).
Notice
No tap or ultrapure water in humidity reservoir.
Sterile distilled water treated to an equivalent quality level is the recommendation for use in the
integrated humidity reservoir. The acceptable conductivity should be within the range 0f 1 to 20
S/cm (resistivity should be within the range of 50 kcm to 1 Mcm). The pH should be in the
range of 7-9. Ultrapure Type 1 water or deionized (DI) water with a resistivity approaching or
equal to 18.2 Mcm, contains very few ions and will actively pull ions from interior
components, damaging stainless steel, copper and glass. If there is only access to DI or Type 1
water, one option is to add a sterile solution of weak sodium bicarbonate to raise the pH and add
ions (recommended 84 mg/l (1mmol/l)).
Notice
Termination of warranty!
Using chlorinated tap water or additives that contain chlorine will void the manufacture
warranty. Similarly, the use of ultrapure water whose conductivity is out of the range of 1 to 20
S (and whose resistivity is out of the range of 50 k to 1 M) will void the manufacture
warranty.
If you should have any questions, please contact Technical Support of Thermo Fisher Scientific.
Notice
No chloride containing disinfectants
While stainless steel is resistant to corrosion, it is not corrosion proof. Many chemicals have a
negative effect on stainless steel, especially chlorine and derivatives with oxidizing activity.
The addition of chloride-containing disinfectants or copper sulfate into the water as a constant
disinfectant is not recommended because these can damage the connecting stainless
steel/copper joint of the drain.
For cleaning the interior, the recommendation is mild soap solution in water then rinse to
remove residues. Wipe the interior surfaces ans parts with a diluted quaternary ammonium
disinfectant. Follow this by wiping with 70 % alcohol to remove any remaining traces of the
disinfectant.
Heracell 150i/240i 50115191_C_01-2018 38 Thermo Scientific
Page 39
Chapter 4 Description of the device
Caution
Risk of an electric shock
Fill the water reservoir just up to the max. level. Overfilling could cause a hazard of the user or a
malfunction of the device (short circuit).
Under normal operating conditions and at the usual incubation temperature of 37 °C, a constant
relative humidity of approx 93 % is achieved in the work space.
If dew formation occurs on the culture containers due to elevated relative humidity, the humidity in
the work space can be adapted to a lower value by enabling the Low Humidity feature. The relative
humidity in the work space is lowered from approx. 93 % to approx. 90 %. The modification
requires an extended adaption phase. To ensure that it effectively prevents dew formation on
culture containers, it must be used as a permanent setting.
CO2 supply:
To ensure the growth conditions for the cell and tissue cultures, the work space is supplied with
CO2.
The pH of the bicarbonate-buffered culture media largely depends on the CO2-content of the work
space atmosphere.
The CO2-content of the work space atmosphere can be controlled within a range of 0 to 20 %.
The supplied CO2 must have one of the following quality characteristics:
• Purity 99.5 % min.
• medical gas quality
O2 supply:
If the CO2-incubator is to be operated with more than 21 % oxygen, the work space is supplied
with oxygen. The O2-content of the work space atmosphere can be controlled within a range of 0
to 21 %.
N2 supply:
If the oxygen content during operation is to be lowered to less than 21 % (air oxygen content), the
work space is supplied with nitrogen. The O
-content of the work space atmosphere can be
2
controlled depending on the configuration of the sensor within a range of 1 % to 21 % and 5 % to
90 %.
Heracell 150i/240i 50115191_C_01-2018 39 Thermo Scientific
Page 40
4.7. Door switch
Chapter 4 Description of the device
Figure. 10. Door switch
A door switch [1] is installed at the upper edge of the work space opening. If the door switch is
activated by opening the glass door, the gas supply and the heating of the work space are
interrupted and the display shows a corresponding message.
If the door remains open for more than 30 seconds, a short audible alarm sounds. If the door
remains open for more than 10 minutes, an audible alarm sounds and the alarm relay responds.
The outer door can only be closed after the glass door has been latched properly.
Note
Versions with gas-tight screen:
For devices with the optional gas-tight screen, the door switch function described above is activated when
the outer door is opened.
Heracell 150i/240i 50115191_C_01-2018 40 Thermo Scientific
Page 41
Chapter 4 Description of the device
4.8. Sensor system
Figure. 11. Temperature, CO
and O2 sensors
2
The blower wheel and two sensor modules are integral to the baseplate [1] of the measuring cell:
• Sensor [2] for the acquisition of the work space temperature and the thermal protection,
•CO2 sensor [3] for the acquisition of the CO2 content in the work space atmosphere.
The O2 sensor (optional) [4] for the acquisition of the O2 content in the work space atmosphere is
installed at various locations next to the measuring cell:
• HERACELL
• HERACELL
®
150i in the sidewall in the upper area [5],
®
240i in the ceiling of the work space [4].
The sensor for the acquisition of the work space temperature as well as the CO2 sensor and the O2
sensor are integral to the control system of the device. Their measured values are compared to the
selected set values. Based on this data, the control system controls heating and CO2/O2/N2
supply.
Heracell 150i/240i 50115191_C_01-2018 41 Thermo Scientific
Page 42
Chapter 4 Description of the device
The blower intermixes the supplied gases and ensures an even temperature distribution within the
work space.
The thermal protection has been preprogrammed at the factory and cannot be changed. It
protects the stored cultures from overheating.
If the set temperature is exceeded by more than 1 °C, the thermal protection responds and the
work space temperature is automatically reduced to the selected set value so that the incubation
process can be continued even in case of a failure. Any response of the thermal protection will
simultaneously trigger a visual alarm.
If the thermal protection is enabled:
• an error message is issued
• the alarm relay responds
If the error message is accepted, the display shows the Over temperature icon to indicate the
response of the thermal protection, and the temperature display is highlighted in red.
Heracell 150i/240i 50115191_C_01-2018 42 Thermo Scientific
Page 43
Chapter 4 Description of the device
4.9. Supply interface
Figure. 12. Supply interface
All supply connections are installed in the supply interface (control box [1]) in the rear of the device.
Heracell 150i/240i 50115191_C_01-2018 43 Thermo Scientific
Page 44
Chapter 4 Description of the device
Gas connection:
The gas supply lines between the device and the gas supply system are connected using the
supplied connecting hoses. O2/N2 is supplied to the device through connecting sleeve [2]. CO2 is
connected to sleeve [3].
All process gases must be supplied to the device at a fixed pressure that has been preset within a
range of 0.8 to 1.0 bar and must remain unchanged.
Before the gases are fed into the work space, they flow through a gas inlet filter with a separation
rate of 99.97 % for a particle size of 0.3 µm (HEPA filter quality).
The illustration shows the optional combined gas connection without the optional gas monitoring
system.
Label:
The label [4] contains information about gas supply, an alarm contact terminal legend, and notes
about the electrical fusing of the device.
RS 232 interface:
Via the RS 232 interface [5], the incubator can be connected to the serial interface of a PC. This
connection allows the computer-aided acquisition and documentation of the major operating
parameters (temperature, CO2/O2/N2 concentration, error codes, etc.).
USB interface (optional):
As an alternative to the RS 232 interface [5], the incubator can be connected to a PC via the
optional USB interface [6]. This connection - USB 1.1 /
USB 2.0 full speed compatible - allows the computer-aided acquisition and documentation of the
major operating parameters (temperature, CO2/O2/N2 concentration, error codes, etc.).
Alarm contact:
The device can be connected to an on-site, external alarm system (e.g. telephone system, building
monitoring system, visual or audible alarm system).
For this purpose, a potential-free alarm contact [7] is preinstalled in the device.
Note
Alarm contact:
The alarm contact responds for all errors reported by the control loops (see „Error messages“ on
page 124).
Power supply connection:
The connection to the power supply system is established by connecting a cable with plug for IEC
connector [9] to the socket [8]. The receptacle for the two device fuses is integral to the socket.
Heracell 150i/240i 50115191_C_01-2018 44 Thermo Scientific
Page 45
Chapter 4 Description of the device
4.10. Work space components
Figure. 13. Work space components
The work space of the incubator has only a minimum of surface, thereby supporting both the
prevention of contamination and easy, effective decontamination.
Heracell 150i/240i 50115191_C_01-2018 45 Thermo Scientific
Page 46
Chapter 4 Description of the device
4.10.1. Interior container
All components of the work space are made of stainless steel and have a high-gloss polished,
absolutely smooth and easy-to-clean surface. Any embossings have a large radius.
As an option, the interior container, the shelf system, and the blower wheel with its cover can be
made of copper material.
Materials of the interior container
The standard version is equipped as follows:
• Interior container made from stainless steel.
Materials with increased contamination protection are optionally available:
• Interior container made from stainless steel, with transparent iONGURAD™ silver ion coating,
• Interior container made from copper, combined with the following components also made from
copper material: Shelf system and blower wheel with protective grid (cover).
Note
Oxidation of copper components:
When exposed to heat and humid air, the copper material of the interior container will oxidize. This results
in a discoloration of the copper components during the test run for checking the device functions. Do not
remove the oxide layer during routine cleaning as the antimicrobial effect of the copper material is based
on it.
The components of the shelf system can be removed easily so that only the easily treatable,
surface-reduced interior container [1] remains in the work space for cleaning and manual
disinfection works.
Heracell 150i/240i 50115191_C_01-2018 46 Thermo Scientific
Page 47
Chapter 4 Description of the device
4.10.2. Optional split gas-tight screens
Equipment versions:
• HERACELL
• HERACELL
®
150i with 3-segment gas-tight screen [2]
®
240i with 6-segment gas-tight screen [3]
For devices that are equipped with the optional gas-tight screen, the contamination hazard is
considerably lower and the recovery times of the incubation parameters are shorter for:
• work space temperature
•CO2 concentration
•O2/N2 concentration
• relative humidity
Advantage of the gas-tight screen: The cross sections of the openings are smaller when samples
are accessed.
Heracell 150i/240i 50115191_C_01-2018 47 Thermo Scientific
Page 48
4.10.3. Water reservoir
Chapter 4 Description of the device
Figure. 14. Water reservoir
The water reservoir [1] is integral to the interior container floor and inclines toward the rear. The
water level is monitored by a water level sensor [2] that issues an alarm message at the display and
an audible signal when the water falls below the minimal level. The embossings [3] in the water tray
are used as indicators for the maximal level.
Heracell 150i/240i 50115191_C_01-2018 48 Thermo Scientific
Page 49
Chapter 4 Description of the device
4.10.4. Gas humidification (optional, only for O2 control)
A hose [5] connects the gas humidification [6] to the device-integral oxygen or nitrogen supply line
[4]. The inflowing oxygen or nitrogen is fed to the heated water. This ensures humidification of the
gases as they enter the work space and prevents an undesired drop of the work space humidity.
4.10.5. Heating system
An air jacket heating is used for heating the work space. The arrangement of the heating elements
ensures that condensate formation above the water reservoir is prevented as far as possible.
The outer door of the device is also heated. The heat radiated onto the interior glass door/gas-tight
screen prevents condensate formation. The work space of the device always remains visible,
despite high humidity.
Heracell 150i/240i 50115191_C_01-2018 49 Thermo Scientific
Page 50
4.10.6. Rear panel openings
Chapter 4 Description of the device
Figure. 15. Rear panel openings
A pressure compensation opening with insert in the rear panel of the device allows a compensation
between the pressures in the work space and in the operating room.
A sealable access port allows cables, hoses or additional sensors to be routed into the work space
of the device.
Heracell 150i/240i 50115191_C_01-2018 50 Thermo Scientific
Page 51
Chapter 4 Description of the device
HERACELL
®
150i:
• access port, Ø 42 mm [1]
• pressure compensation opening [2]
HERACELL
®
240i:
• access port, Ø 42 mm [3]
• pressure compensation opening [4]
Note
Operating conditions:
When accessories are to be operated in the work space of the CO2-incubator, the ambient condition
requirements must be observed (see table below). The energy introduced into the work space affects the
beginning of the temperature control range. When additional heating sources are introduced into the work
space, condensation (e.g. at the glass door) may occur.
Introduced energy Control ranges of the temperatures
General Example:
1
RT
= 21 °C
0W RT + 3°C 24°C
5W RT + 6,5°C 27,5°C
10 W RT + 9,5 °C 30,5 °C
15 W RT + 13 °C 34 °C
20 W RT + 16 °C 37 °C
1 RT= Ambient temperature
4.10.7. Shelf system
The support rails [1] of the shelf system are perforated every 42 mm so that the support hooks [8]
can be inserted variably for any culture container size required. The shelves [2] have an integrated
tilt protection and withdrawal stop. For details of the shelf system, see Section „Start-up“.
Heracell 150i/240i 50115191_C_01-2018 51 Thermo Scientific
Page 52
4.10.8. Bottle turning device for HERACELL® 240i (optional)
Chapter 4 Description of the device
Figure. 16. Bottle turning device
HERACELL
®
240i may be equipped with up to four separate bottle turning devices. Each unit consists
of a roller insert [6] with a drive roller [4] and four idle rollers [7] that are also rotated due to the
transmission function of the inserted cylinders [5].
Heracell 150i/240i 50115191_C_01-2018 52 Thermo Scientific
Page 53
Chapter 4 Description of the device
Each unit is controlled separately as the drive roller is connected to the device control unit by a
connector [3] at the right side of the device.
This modular layout also allows the operation of only one bottle turning device in combination with
several shelves.
The arrangement of the idle rollers in the roller insert frame depends on the configuration of the
cylinders used. The drive roller is always inserted into the right roller guide. The position and the
number of the idle rollers in the frame then depend on the cylinder diameter and on the number of
cylinders. The figure shows three examples of different idle roller positions in dependence of the
cylinder diameter.
The arrangement of the roller inserts in the work space is designed alphabetically as a, b, c, and d
with a being the lowest and d being the uppermost roller insert.
The connections of the drive rollers are assigned correspondingly to the device control unit. The
drive roller of the switching insert a must only be connected to the lower receptacle a of the
corresponding control unit.
Note
Connection assignment:
If the drive roller is not properly connected to the control unit, the parameters cannot be set correctly on
the operating panel of the device.
Receptacles:
To prevent corrosion due to moisture in the work space, unassigned receptacles of the bottle turning
device must always be plugged with the protective cap.
The rotating speed of the bottle turning devices is set depending on two parameters:
• Diameter (of the cylinders used),
• drive roller set value.
The value is determined using a diagram (see „Installing the optional bottle turning devices
HERACELL® 240i“ on page 62).
Heracell 150i/240i 50115191_C_01-2018 53 Thermo Scientific
Page 54
4.10.9. Water pump
Chapter 4 Description of the device
Figure. 17. Water pump
Heracell 150i/240i 50115191_C_01-2018 54 Thermo Scientific
Page 55
Chapter 4 Description of the device
The water pump serves for suction cleaning of the remaining water in the water reservoir. The
water drain works through gravity.
1. Place the water pump [1] on lowest shelf [5].
2. Feed the inlet hose [4] through a whole [7] of the lowest the shelf to the water reservoir [8].
3. Prepare a bucket [2].
4. Suck the water, therefore pump the water pump using the hand grip [6] about four times until
water flows in the outlet hose.
5. Let the water run into the bucket [2] through gravity.
6. Wipe out the rests of the water at the floor plate of the water reservoir.
Heracell 150i/240i 50115191_C_01-2018 55 Thermo Scientific
Page 56
Chapter 4 Description of the device
Heracell 150i/240i 50115191_C_01-2018 56 Thermo Scientific
Page 57
Chapter 5 Start-up
5. Start-up
5.1. Let the device adapt to the ambient conditions
Let the device adapt to the ambient conditions!
The device must adapt to ambient conditions prior to start-up.
• Set up the device in the operating room and let it adapt to the expected operating room
temperature.
• For a period of approx. 2 hours prior to switching it on.
• Open the device doors.
5.2. Preparing the work space
Upon delivery, the CO2 incubator is not in a sterile state. Before the initial start-up, the device must
be decontaminated.
Before the decontamination is performed, the following work space components must be cleaned:
• Support rails,
• support hooks,
• shelves,
• bottle turning assembly (optional),
• gas humidification (optional),
• work space surfaces,
• work space seals and gaskets,
• glass door/gas-tight screen.
Note
Decontamination:
For details about the cleaning and disinfection of the device (see „Cleaning and disinfection“ on
page 133).
Heracell 150i/240i 50115191_C_01-2018 57 Thermo Scientific
Page 58
Chapter 5 Start-up
5.3. Installing the shelf system
Caution
Risk of overloading!
Overloading may damage the shelves or cause the shelves and/or the CO2-incubator to tilt.
When the shelves are being drawn out, ultimately destroying the samples.
To avoid overloading the incubator or its shelving be sure to observe the sample weight limits
specified in chapter 12.
Tools are not required for the installation of the shelf system. The support rails are secured using
spring pressure. After the support hooks have been inserted into the rail, the shelves are pushed
onto the support hooks.
Heracell 150i/240i 50115191_C_01-2018 58 Thermo Scientific
Page 59
Chapter 5 Start-up
5.3.1. Installation/removal of the support rail
Figure. 18. Support rail installation/removal
The support rails are held at the sides by the embossings [2] and [5] and secured by the
embossings [1] and [6]. The support rails marked with the diamond shape are inserted at the rear
panel of the device with the locksprings [3] facing upward.
1. Position the support rail [4] onto the lower embossing [6] and tilt it toward the work space side
wall so that the rail is positioned over the two embossings [5] and [2].
2. Clamp the lockspring [3] behind the upper embossing [1].
3. To remove the support rails, pull the lockspring tab down out of the embossing and remove
the rail.
Heracell 150i/240i 50115191_C_01-2018 59 Thermo Scientific
Page 60
5.3.2. Installing the shelf supports:
Chapter 5 Start-up
Figure. 19. Shelf support installation
1. Insert the shelf supports [3] into the perforations [1] of the support rail with the bar facing
down.
2. Make sure that the two vertical elements [2] of the shelf support are flush with the support rail.
Installing the shelves:
1. Push the shelf [4] onto the shelf supports with the tilt protection [5] facing the rear panel of the
device. The tilt protection [5] is also used as a guide for the shelf.
2. Slightly raise the shelf so that the withdrawal stop [6] can be routed over the shelf supports.
3. Make sure that the shelf support is positioned in the two tilt protections in a way that allows it
to move freely.
5.3.3. Leveling the device
1. Position a bubble level onto the center shelf or onto the roller holder.
2. Rotate the adjustable device stands using the supplied 24 mm wrench until the shelf is
positioned horizontally in all directions. Perform the adjustment of the device stands from left
to right and from rear to front.
Heracell 150i/240i 50115191_C_01-2018 60 Thermo Scientific
Page 61
Chapter 5 Start-up
5.4. Installing the gas humidification
(optional, only for O
2/N2
-control)
Figure. 20. Installing gas humidification
The gas humidification [1] is installed to the water tray parallel to the rear panel of the device. The
position to the right side wall is predetermined by the hose length.
1. Install hose [2] to the sleeve of the gas humidification and then to the sleeve [3] of the
device-integral oxygen or nitrogen supply line.
2. Place gas humidification immediately to the rear panel of the device and align it parallel to the
rear panel.
Heracell 150i/240i 50115191_C_01-2018 61 Thermo Scientific
Page 62
Chapter 5 Start-up
5.5. Installing the optional bottle turning devices HERACELL® 240i
Figure. 21. Installing the bottle turning devices
1. Align the roller insert so that the roller holder [8] and the cross member [7] are flush and facing
the work space opening.
2. Slide the roller insert with both guide rails [5] onto the shelf supports.
3. Slightly raise the roller insert so that the withdrawal stop can be routed over the shelf support.
4. Insert the drive roller [6] into the right receptacle and connect the cable [4] to the
corresponding control unit a.
5. Insert the four idle rollers [3] the into receptacles and position the rollers in the receptacles in
accordance with the bottle diameter.
6. Place the bottles onto the rollers. To ensure that the bottles will not be shifted against each
other by the rotation, the bottoms of the bottles should contact the stop disks [2] of the rollers
and the cylinder necks [1] should contact the rubber stop rings [8]. The stop ring can be
positioned accordingly on the roller.
Set speed of the bottle turning device:
7. The rotating speed of the bottle turning devices is set depending on two parameters:
• Diameter (of the bottles used),
• drive roller set value.
Use of diagram:
8. The diagram shows the common bottle diameters as different graphs (ascending straight).
• Set the desired speed at the Y axis. Read the corresponding set value (in %) from the X axis at
the point of intersection of the Y value with the specific graph.
Heracell 150i/240i 50115191_C_01-2018 62 Thermo Scientific
Page 63
Chapter 5 Start-up
Figure. 22. Bottle turning speed
Example:
• Bottle diameter: 56 mm
• Desired speed: 3.5 rpm
• Determined set value: 48 %
In the dialog box SPEED BOTTLE TURN. DEVICE, the value 48 % is set (see „Options“ on
page 110).
Heracell 150i/240i 50115191_C_01-2018 63 Thermo Scientific
Page 64
Chapter 5 Start-up
5.6. Installing the optional center strut HERACELL® 240i for split
inserts
Figure. 23. Center strut installation
If HERACELL
®
240i is equipped with the optional 6-segment insert, two center struts [1] with
perforations at each side must be installed. In this case the shelf support rails must be installed to
the support rails at the sides and at the left and right side of the strut.
The support rails marked with the diamond shape are installed at the rear panel of the device.
1. A pressure spring keeps the upper rectangular guide [3] of the center strut under tension.
Insert this guide into the receptacle [2] in the work space ceiling and push it slightly upward so
that it is pushed into the center strut.
2. Then, position the lower rod guide [5] of the center strut into the hole [6] in the work space
floor and remove spring tension.
3. The center strut is stabilized by the spring pressure.
Note
Configuration with split inserts:
For versions with split inserts, bottle turning devices cannot be installed.
Heracell 150i/240i 50115191_C_01-2018 64 Thermo Scientific
Page 65
Chapter 5 Start-up
5.7. Gas connection
Note
Gas quality:
The gases must have one of the following qualities:
• Purity 99.5 % min,
• medical gas quality.
Caution:
Overpressure!
The operating pressure of the gas applied to the device must not exceed 1 bar. If the gas is
supplied at a higher pressure, the valves integral to the device may not close correctly and the
gas supply control may be impaired.
Set the gas supply to a range between 0.8 bar min. and 1.0 bar max. and make sure that this
pressure setting cannot be changed!
5.7.1. Installing gas pressure hoses
Figure. 24. Installing gas pressure hoses
Heracell 150i/240i 50115191_C_01-2018 65 Thermo Scientific
Page 66
Chapter 5 Start-up
The gas supply from the gas supply system to the device is achieved using the supplied flexible
gas pressure hoses:
1. Connect the gas pressure hose [2] to the sleeve of the gas supply system.
2. Remove the protective cap [3] from the sterile filter.
3. Slide the hose clamp [1] onto the gas pressure hose and connect hose to the sleeve [4] of the
sterile filter [5].
4. Secure the gas pressure hose to the sleeve of the sterile filter using the hose clamp.
Note
Pressure compensation opening:
To ensure permanent pressure compensation, the pressure compensation opening must not be connected
to an exhaust air system. The pipe of the pressure compensation opening must not be extended or
redirected.
5.7.2. Installing devices without the optional gas monitoring system
Figure. 25. Gas connection without gas monitoring system
Heracell 150i/240i 50115191_C_01-2018 66 Thermo Scientific
Page 67
Chapter 5 Start-up
The gas supply from the gas supply system to the device is established using the supplied flexible
gas pressure hoses (see „Installing gas pressure hoses“ on page 65):
CO2 connection:
• For a device with CO2 connection, connect the gas supply to the sterile filter [2] in accordance
with the connection diagram [1].
Combined CO2 and O2/N2 connection (optional):
For a combined CO2/O2/N2 connection, proceed as follows in accordance with the connection
diagram [3]:
• Connect the O2/N2 supply line to the upper gas inlet filter [4],
• connect the CO2 supply line to the lower gas inlet filter [5].
Note
Manual gas monitoring:
On devices without the optional gas monitoring system, there is no automatic gas monitoring.
Therefore, the filling level of the gas bottles must be checked every day.
5.7.3. CO2 connection with gas monitoring system (optional)
Figure. 26. Gas connection with gas monitoring system (optional)
The gas supply from the gas supply system to the device is established by the supplied flexible gas
pressure hoses.
Heracell 150i/240i 50115191_C_01-2018 67 Thermo Scientific
Page 68
Chapter 5 Start-up
Devices with CO2 connections that are equipped with the optional gas monitoring system are
connected according to the connection diagram [7].
CO2 connection:
For a device that is operated with process gas CO2 and equipped with the optional gas monitoring
system, connect the gas supply lines as follows:
• Distributor connection [5] for the CO2 gas supply for up to three additional devices. For looping
the gas supply through, interconnect the devices with a gas pressure hose.
• Connect the gas container B to the upper connection [2] of the gas monitoring system.
• Connect the gas container A to the lower connection [3] of the gas monitoring system.
• The outlet of the gas monitoring system [4] is connected at the factory to the gas inlet filter [5] by
a short gas pressure hose [6].
Note
Looping the gas supply through:
By looping the gas supply through, up to 4 devices can be supplied by a CO2 gas supply.
5.7.4. Combined CO2/O2/N2 connection with gas monitoring system (optional)
Figure. 27. Combined connection with gas monitoring system (optional)
Heracell 150i/240i 50115191_C_01-2018 68 Thermo Scientific
Page 69
Chapter 5 Start-up
The gas supply from the gas supply system to the device is established by the supplied flexible gas
pressure hoses.
Devices with the combined CO2/O2/N2 connection that are equipped with the optional gas
monitoring system are connected according to connection diagram [13] and have the following
gas connections:
CO2/N2 supply:
• Distributor connection [1] for the O2/N2 gas supply for up to three additional devices. For
looping the gas supply through, interconnect the devices with a gas pressure hose.
• Connect the gas container B [2] to the upper connection of the gas monitoring system.
• Connect the gas container A [3] to the lower connection of the gas monitoring system.
• The outlet of the gas monitoring system [4] is connected at the factory to the gas inlet filter [9] by
a short gas pressure hose [11].
CO2 supply:
• Distributor connection [5] for the CO2 gas supply for up to three additional devices. For looping
the gas supply through, interconnect the devices with a gas pressure hose.
• Connect the gas container B to the upper connection [6] of the gas monitoring system.
• Connect the gas container A to the lower connection [7] of the gas monitoring system.
• The outlet of the gas monitoring system [8] is connected at the factory to the gas inlet filter [10]
by a short gas pressure hose [12].
Note
Looping the gas supply through:
By looping the gas supply through, up to 4 devices (see annex at the end of this section) can be supplied
by a CO2 gas supply or O2/N2 gas supply.
5.8. Power supply connection
Warning
Electric shock!
Contact with current-carrying components may cause a lethal electric shock.
Before connecting the device to the power supply, check plug and power supply cable for
damage.
Do not use damaged components when connecting the device to the power supply!
The device must be connected only to a correctly installed and grounded power supply source:
• Fusing T 10 A
• Circuit breaker G 16
Heracell 150i/240i 50115191_C_01-2018 69 Thermo Scientific
Page 70
Chapter 5 Start-up
Figure. 28. Power supply connection
Connection to the power supply system:
1. Before connecting the device to the power supply, check to see if the voltage of the power
supply corresponds with the specifications on the nameplate on the front of the device. If the
ratings given for voltage (V) and current (A) are not correct, the device must not be connected
to the power supply.
2. Connect the IEC connector [2] to the socket [1] of the control box of the device.
3. Route the power cord along a path that does not cross hot spots (e.g. drainpipes), tables,
passageways and aisles. With stacked devices, keep the power cord away from hot spots on
the other refrigerated incubator in the stack.
4. Connect the grounding plug [3] of the power supply cable to a correctly grounded and fused
socket.
5. Make sure the power supply cable is not subjected to tensile or compressive force.
Note
Make sure that power sockets remain freely accessible at all times!
To allow a rapid disconnection of power in case of an emergency, make sure that power outlets remain
freely accessible at all times!
Heracell 150i/240i 50115191_C_01-2018 70 Thermo Scientific
Page 71
Chapter 5 Start-up
5.9. Connecting the RS 232 interface
Notice
Replacement should only be carried out by skilled and authorized
qualified personnel of electrotechnology/signal engineering
The RS-232 data communication interface supports the querying of status information and
temperature data from the CO2-Incubator by entering basic commands in a standard terminal
window provided by your computer’s operating system. The interconnection requires a standard
RS-232 cable with 9-pin connectors and a straight “1:1” pinout without any crossed wires, which
is not supplied with the Incubator.
Notice
RS-232 interface compatibility
To avoid overloading and damaging the RS-232 interface check the interfacing parameters
against the pin-out description given above and make sure that computer’s interface port works
with a signal level of ±5 V DC. The RS 232 data communication interface has been designed for
a cable connection with 9-pin connector and a contact assignment of 1:1.
Data exchange is accomplished by a predefined structure of command sequences (see „Data
communication“ on page 165).
Note
Data communication diagram:
The command sequence syntax as well as the RS 232 interface data communication diagram are
described in the annex at the end of this section.
Connecting the devices:
1. Turn the PC off.
2. Connect the serial interface cable connector (not included in the standard equipment) to the
socket at the device supply interface at the rear of the device.
3. Connect the second connector to an unassigned serial port COM 1/COM 2 etc. at the PC.
4. Turn the PC on.
Heracell 150i/240i 50115191_C_01-2018 71 Thermo Scientific
Page 72
Chapter 5 Start-up
5.10. Connecting the USB interface (optional)
As an alternative to data communication via RS 232 interface, the devices can be equipped with
the optional USB interface. The USB interface complies with Standard USB 1.1 and is compatible
with Standard USB 2.0 (full speed).
The interface stepping rate can be changed within the defined baud rates (9,600, 19,200, 38,400,
57,600 baud).
Data exchange is accomplished via a defined command sequence structure (frames). The
command sequences correspond with the RS 232 interface installation diagram (see annex at the
end of this section).
Using the USB interface for data communication between a PC and the device a driver must be
installed first (see annex data communication).
5.11. Connecting the alarm contact
Note
Skilled work:
Thermo Scientific warrants the operational safety and the operativeness of the device only if installation
and repairs are performed properly.
The connection of the device to an external alarm system must only be carried out by adequately trained
and authorized expert electrical/telecommunication personnel!
Function:
When system errors and failures occur in the temperature or gas control circuits, an alarm
message is issued to the connected alarm/monitoring system. The potential-free contact (1
changeover contact) has been designed for the following circuits:
Alarm relay:
Circuit Voltage External fusing
Circuits with system voltage max. 250 V ~ max. 6 A
SELV circuits (cf. VDE 0100, Part 410) 25 V max. 2 A
60 V max.1 A
SELV-E circuits (cf. VDE 0100, Part 410) 50 V max. 1 A
120 V max. 0,5 A
Operating states Contact 4- 1 Contact 4 -3
Operating state power failure "off" X O
Operating state power failure "on" O X
Failure: power failure "off" O X
Failure: power failure "on" X O
X: Contact closed/O: Contact open
Heracell 150i/240i 50115191_C_01-2018 72 Thermo Scientific
Page 73
Chapter 5 Start-up
Note
Switching structure:
The alarm relay switches for all failures reported by the control loops (see „Error messages“ on
page 124).
Caution
Alarm contact electrical compatibility considerations
To avoid overloading and damaging the alarm contact, check the electrical interfacing
parameters of the alarm-receiving system for compatibility with the alarm-relay specifications
given above.
Note
Blackout:
If the alarm relay reports a blackout, no display appears and the switches do not blink.
Heracell 150i/240i 50115191_C_01-2018 73 Thermo Scientific
Page 74
Connection example:
Chapter 5 Start-up
Figure. 29. Connection example
The connector [5] for the connecting cable is included in the standard equipment. The values for
the operating voltage of the external circuits and of the fusing of the alarm system are given in the
table on the previous page.
1. Connect the individual conductors [1] to [4] of the connecting cable as shown in the wiring
diagram.
2. Route the alarm cable along a path that does not cross possible hot surfaces (e.g. exhaust air
piping) tables, passageways and aisles. With stacked devices, keep the alarm cable away
from hot spots on the other CO2-incubator in the stack.
3. Connect the connector [5] of the alarm system connecting cable to the interface [6] at the
control box at the rear panel of the device.
Heracell 150i/240i 50115191_C_01-2018 74 Thermo Scientific
Page 75
Chapter 6 Operation
6. Operation
Caution
The microbiological safety of the unit is largely dependent on the proper conduct of the persons
using the unit. Refer to the Appendix "Basic rules of sound microbiological engineering
practice".
6.1. Prior to starting operation
Apply the required individual protective equipment (e.g. for hands, face, body) and take off all
jewelery.
6.2. Preparing the device
The device must only be released for operation after all major measures for the start-up have been
taken (see Section 5).
Device check:
Prior to starting operation, the following device components must be checked for their correct
function
• Gas hoses must be seated tight on the connecting filter and must be secured using a hose
clamp.
• The access port must be capped.
• The pressure compensation opening must be permeable, its insert must be installed in the work
space.
• The glass door/gas-tight screen seal must not be damaged.
• The glass door measurement opening must be capped.
• The shelf system components must be installed safely.
• The gas humidification (optional) must be connected to the device-integral gas supply and
aligned parallel to the rear panel.
• The drive rollers of the bottle turning device (optional) must be properly connected to the
corresponding control unit.
• The idle rollers of the bottle turning device (optional) must be positioned in accordance with the
cylinder diameters.
• The glass door must not be damaged.
• Disinfecting and cleaning the incubators work space with fuzz-free materials.
• Disinfect the work space according to the operator-specified hygiene guidelines.
• Don‘t use any explosive disinfectants. When using alcohol as a disinfectant, attend the national
regulations.
Heracell 150i/240i 50115191_C_01-2018 75 Thermo Scientific
Page 76
Chapter 6 Operation
Decontaminating the device work space:
Run the contra-con decontamination routine (see „contra-con decontamination routine“ on
page 137) or decontaminate the work space according to the hygiene regulations set forth by the
operator.
Note
Hygiene regulations:
Prior to any operation, the user must clean and disinfect the work space in accordance with the hygiene
regulations set forth by the operator to protect the cultures.
The “Principles of good microbiological proceedings” at the annex of these instructions can be used as
safety information for personnel working with the device.
Water supply:
A sufficient quantity of treated water of the following quality must always be available for the running
operation:
• distilled, fully demineralized, and
• autoclaved, sterile.
When the filling level drops below a minimum value, it must be possible to top up water during the work
process.
Capacities:
• HERACELL
• HERACELL
®
150i: 3.0 l
®
240i: 4.5 l
6.3. Starting operation
1. Fill the water tray with a sufficient quantity of processed water. Do not exceed the upper level
mark.
2. Make sure that the CO2/O2/N2 supply system valves are open.
3. Turn the device on using the power switch.
4. Set the set values for temperature and CO2/O2 content on the operating panel.
5. Start contra-con.
Starting the device:
6. Start the device with the auto-start routine (see „Activating auto start“ on page 94).
7. The progress indicator auto-start appears on the display, and the automatic start routine is
run.
8. The temperature control adjusts the temperature to the selected set value, humidity rises.
9. When temperature and relative humidity are constant, the automatic adjustment of the
CO2/O2 measuring system is performed.
10. The CO2/O2 control supplies the set amount of CO2/O2.
Heracell 150i/240i 50115191_C_01-2018 76 Thermo Scientific
Page 77
Chapter 6 Operation
11. If the auto-start routine has been completed, the progress indicator is hidden, and the main
menu is displayed. The device is operational.
Caution!
To avoid any risk of explosion and fire:
• refrain from loading the CO2-incubator with any of the substance listed in the section
„Incorrect use“ on page 14.
• make sure that the ambient air is free of any solvents!
• do not operate the CO2-incubator in areas with an explosion hazard!
Loading the device:
12. Load the work space with cultures or place bottles onto the bottle turning device rollers.
Note
Duration of the auto-start routine:
When the device is cold and when the ambient temperature is low, the auto-start routine may take up to
10 hours.
Loading:
To ensure sufficient air circulation and even heating of the samples, the loading surface within the work
space should be used up to 70 % max. Voluminous objects in the work space that dissipate heat may
impair heat distribution.
During tempering of defines substances or materials with higher moisture content, the access port is to be
opened in order to reduce a possible condensation in the chamber. If necessary a modified door seal is
available as an accessory.
Caution
Hot surfaces!
The screen of the glass door, the interior panel of the outer door as well as the surfaces of the
shelving and the work space reach temperatures up to 55 °C while the CO2-incubator is running
through its heating cycles and need some time to cool down.
When removing samples from a running or recently completed heating cycle, always wear
safety gloves and other appropriate personal protection equipment to avoid burns on hot
surfaces.
Caution
Risk of overloading!
Overloading may damage the shelves or cause the shelves and/or the CO2-incubator to tilt.
When the shelves are being drawn out, ultimately destroying the samples.
To avoid overloading the incubator or its shelving be sure to observe the sample weight limits
specified in chapter 12.
Heracell 150i/240i 50115191_C_01-2018 77 Thermo Scientific
Page 78
Chapter 6 Operation
6.4. Interruption of operation
Make sure to prevent Contamination when operation is interrupted.
1. Remove all samples an brought in tools from the device, then clean and disinfect them.
2. Clean and disinfect the internal space.
3. Eliminate any debris.
4. Decontaminate the device.
Heracell 150i/240i 50115191_C_01-2018 78 Thermo Scientific
Page 79
Chapter 7 Handling and control (iCanTM touchscreen controller)
7. Handling and control (iCanTM touchscreen
controller)
7.1. Power switch
Figure. 30. Power switch
Depending on which side the door hinges are installed, the power switch [1] is integral to the front
cover [2] of one of the front device stands.
• To turn the device on: Press the power switch [1]; the switch illumination comes on.
After a short audible signal and a short dark phase, the touchscreen display appears.
The control loop sensors pass the heat-up phase (see „Heat-up phase of the control loop
sensors“
• To turn the device off: Press the power switch; the switch illumination goes off.
on page 84).
Heracell 150i/240i 50115191_C_01-2018 79 Thermo Scientific
Page 80
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.2. Operating panel and operating structure
Figure. 31. Pressure-sensitive areas
The control panel works as a touchscreen and can be controlled performing light pressure on the
pressure-sensitive areas of the screen using a finger or a blunt pin:
• Temperature display [1]
•CO2 display [3]
•O2 display [5] (optional)
• contra-con key [2]
• menu key [4]
• auto-start key [6]
Note
Extended pressure-sensitive area:
To accept a failure message, the entire touchscreen can be used as a pressure-sensitive area.
Heracell 150i/240i 50115191_C_01-2018 80 Thermo Scientific
Page 81
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.3. Versions without O2/N2 control
Figure. 32. iCan
TM
touchscreen without O2/N2 gas supply
Function keys and value displays of the operating panel for a device version without O2/N2 gas
supply:
1. Type designation of the device
2. Temperature display
3. CO2 display
4. Key for starting the contra-con decontamination routine
5. Key for opening the menu navigation
6. Key for starting the auto-start routine
7. Actual value CO
8. Set value CO
2
2
9. Temperature actual value
10. Temperature set value
11. Current date and time
Heracell 150i/240i 50115191_C_01-2018 81 Thermo Scientific
Page 82
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.4. Version with combined CO2/O2/N2 control (optional)
Figure. 33. iCan
TM
touchscreen with combined gas connection
Function keys and value displays of the operating panel of a device version with combined
CO2/O2/N2 gas supply:
1. Type designation of the device
2. Temperature display
3. CO2 display
4. CO2 set value
5. CO2 actual value
6. Key for starting the contra-con decontamination routine
7. Key for opening the menu navigation
8. Key for starting the auto-start routine
9. O
10. O
display
2
actual value
2
11. O2 set value
12. Temperature actual value
13. Temperature set value
14. Current date and time
Heracell 150i/240i 50115191_C_01-2018 82 Thermo Scientific
Page 83
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.5. Structure of the operating levels
Operation is divided into three levels:
• A: Direct access to the control loop settings: Temperature, CO2, O2 set value and bottle turning
device (optional),
• B: Start of the device routines contra-con or auto-start,
• C: Navigation through the submenus for device configuration.
Figure. 34. Menu structure overview
Heracell 150i/240i 50115191_C_01-2018 83 Thermo Scientific
Page 84
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.6. Factory presettings of the iCanTM touchscreen controls
Upon delivery of the device, the following set values have been preset:
• Temperature: 37 °C
•CO2 content: 0.0 %
•O2 content (optional): 21.0 %
• Optional bottle turning device speed a, b, c, and d: 0 %.
Note
CO2/O2 control:
Since the CO2 concentration of the air is nearly 0 %, the CO2 control and the control loop error monitoring
system are disabled at a set value of 0 %.
Since the O2 concentration of the air is nearly 21 %, the O2 control and the control loop error monitoring
system are disabled at a set value of 21 %.
7.7. Heat-up phase of the control loop sensors
Figure. 35. Heat-up phase display
When the device has been switched on, the control loop sensors pass through a heat-up phase of
varying duration during the start process:
Temperature control loop 10 s
control loop 10 s
CO
2
CO2 control loop with IR measuring cell 3 min
O2 control loop 5 min
Heracell 150i/240i 50115191_C_01-2018 84 Thermo Scientific
Page 85
Chapter 7 Handling and control (iCanTM touchscreen controller)
The start process is indicated by an audible signal. During the heat-up phase, the displays show
dots (...) instead of values:
• Temperature display [2]
•CO2 display [1]
•O2 display [3]
After the heat-up phase has been completed, the control loop actual values are indicated.
Note
Exposure to CO2 gas:
During the 5-minute heat-up phase of the O2 control loop, the work space is not exposed to CO2 gas and
the CO2 control and is disabled.
7.8. Event actions for settings
Key response:
Pressing a key can increase or reduce a value gradually:
• When the – key [4] or the + key [6] is kept depressed, the system switches to quick run,
• after the key has been kept depressed for more than 3 seconds, the quick run speed increases.
Note
Saving the settings:
To save changed values, press the Enter key.
Resetting the settings:
Unless a user action (contact with the pressure-sensitive areas and keys) occurs within 30 seconds, the
system automatically exits the menu and the most recently confirmed setting defaults.
Heracell 150i/240i 50115191_C_01-2018 85 Thermo Scientific
Page 86
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.9. Setting the temperature set value
Figure. 36. Setting the temperature set value
1. Press the TEMPERATURE DISPLAY key [1].
The Temperature menu is displayed.
2. To exit the Temperature menu:
Press the END key [2].
3. To set the temperature set value:
Press the SET VALUE key [3].
Heracell 150i/240i 50115191_C_01-2018 86 Thermo Scientific
Page 87
Chapter 7 Handling and control (iCanTM touchscreen controller)
To increase the set value:
Press the + key [6].
To reduce the set value:
Press the - key [4].
4. To accept and save the set value:
Press the ENTER key [5].
The system returns to the main menu. The temperature displays shows the actual value
currently measured in the work space.
Heracell 150i/240i 50115191_C_01-2018 87 Thermo Scientific
Page 88
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.10. Setting the CO2 set value
Figure. 37. Setting the CO
set value
2
1. Press the CO2 DISPLAY key [1].
The CO
menu is displayed.
2
2. To exit the CO2 menu:
Press the END key [2].
3. To set the CO
set value:
2
Press the SET VALUE key [3].
To increase the set value:
Press the + key [6].
Heracell 150i/240i 50115191_C_01-2018 88 Thermo Scientific
Page 89
Chapter 7 Handling and control (iCanTM touchscreen controller)
To reduce the set value:
Press the - key [4].
4. To accept and save the set value:
Press the ENTER key [5].
The system returns to the main menu. The CO2 display shows the actual value currently
measured in the work space.
Note
Deactivating the CO2 control loop:
To deactivate the CO2 control, the set value is set to 0 %.
If the control loop is deactivated, error monitoring is enabled as well.
Gas monitoring:
The switching state of the CO2 control loop does not affect the function of the optional gas monitoring
system. The gas monitoring system remains active even when the CO2 control is switched off.
For set values of 21 % O2, the O2 control is switched off and error monitoring is disabled as well.
The control loop error monitoring system (optional) is enabled.
Heracell 150i/240i 50115191_C_01-2018 89 Thermo Scientific
Page 90
7.11. Setting the O2 set value
Chapter 7 Handling and control (iCanTM touchscreen controller)
Figure. 38. Setting the O
set value
2
This setting is possible only on versions with the optional O2/N2 control.
1. Press the O2 DISPLAY key [1].
The O2 menu is displayed.
2. To exit the O2 menu:
Press the END key [2].
3. To set the O2 set value:
Press the SET VALUE key [3].
Heracell 150i/240i 50115191_C_01-2018 90 Thermo Scientific
Page 91
Chapter 7 Handling and control (iCanTM touchscreen controller)
To increase the set value:
Press the + key [6].
To reduce the set value:
Press the - key [4].
4. To accept and save the set value:
Press the ENTER key [5].
The system returns to the main menu. The O2 displays shows the actual value currently
measured in the work space.
Note
Factory settings:
Depending on the type of the O2 sensor, one of the two O2 control ranges has been preset at the factory:
Control range I: 1 % to 21 %
Control range II: 5 % to 90 %
Usage of the process gases:
For set values below 21 % O2, the device must be connected to a nitrogen supply system.
For set values above 21 % O2, the device must be connected to an oxygen supply system.
Since the O2 concentration of the air is nearly 21 %, the O2 control and the control loop error monitoring
system are disabled at a set value of 21 %.
The control loop error monitoring system (optional) is enabled.
7.12. Auto-start routine
The auto-start function is an automated routine for the start and the subsequent adjustment of the
CO2 measuring system. After the start, the device control adjusts the temperature to the set value
while humidity is generated. When temperature and relative humidity have reached constant
values, the CO2 measuring system is automatically adjusted to these values, and the work space is
supplied with the preset quantity of CO2.
Heracell 150i/240i 50115191_C_01-2018 91 Thermo Scientific
Page 92
Chapter 7 Handling and control (iCanTM touchscreen controller)
Note
Application of the auto-start routine:
To ensure that the specified accuracy of the CO2 measuring system is maintained, the device should
always be started using the auto-start routine if
• a difference of more than 1 °C is entered upon setting the temperature set value,
• the Low Humidity function is enabled/disabled,
• the device is restarted after an extended interruption of operation.
The auto-start routine should be run at least every three months on the occasion of cleaning and
maintenance works.
Duration of the routine:
Running the routine usually takes 5 to 7 hours. At low room temperatures and when the device is cold, it
may take up to 10 hours until the auto-start routine has been completed. If the glass door is opened or if
the power supply of the device is interrupted while the routine is running, the routine is interrupted and
rerun after the glass door has been closed or the power supply has been reestablished.
Start conditions for the auto-start routine:
Prior to running the auto-start routine, set the CO2 and O2 set values to the desired values and make sure
that the atmosphere in the work space only consists of ambient air.
The floorpan of the device must be filled with a sufficient quantity of water.
Conditions that prevent the start of the auto-start routine:
The auto-start routine cannot be started if one of the following failure conditions exists.
Temperature control loop:
• Sensor breakage
• actual value above set value (excessive deviation)
• actual value below set value (excessive deviation)
• set value not plausible
• calibration values too high or too low
• sensor communication failure
• sensor parameter not plausible
• no communication with LM 75
Heracell 150i/240i 50115191_C_01-2018 92 Thermo Scientific
Page 93
Chapter 7 Handling and control (iCanTM touchscreen controller)
Note
Control loop for CO2 gas supply:
• No communication with sensor.
In this case, the auto-start key is dimmed and its function is not available.
Faulty cancellation of the auto-start routine:
The auto-start is canceled if:
• an error is detected in the temperature control loop,
• an error is detected in the CO2 control loop,
• the water filling level is insufficient.
auto-start-Dry execution of auto-start routine:
If the auto-start routine is to be run dry (without water in the floorpan of the work space), the water level
sensor must be disabled prior to its start (see „Options“ on page 110).
Heracell 150i/240i 50115191_C_01-2018 93 Thermo Scientific
Page 94
7.12.1. Activating auto start
Chapter 7 Handling and control (iCanTM touchscreen controller)
Figure. 39. Activating the auto-start routine
7.12.1.1. Preparations for the start:
1. Make sure that the CO2-/O2-/N2 gas supply system valves are open.
2. Fill the floorpan of the work space with a sufficient quantity of water. The filling level must not
exceed the upper filling level mark.
3. Set the set values for temperature, CO2 and O2 on the touchscreen.
7.12.1.2. auto-start routine activation:
1. Press the auto-start key [2].
Heracell 150i/240i 50115191_C_01-2018 94 Thermo Scientific
Page 95
Chapter 7 Handling and control (iCanTM touchscreen controller)
The auto-start-instruction menu [1] is displayed.
2. To exit the auto-start-instruction menu and cancel auto-start:
Press the END key [4].
3. Activate the auto-start routine:
Press the START key [3].
4. To air the work space, open both device doors.
5. When the audible alarm sounds after 30 seconds, close both device doors.
The trend status indicator [5] is displayed.
6. During the progress, the status indicator displays the following information:
Temperature,
start time.
Note
Cancellation:
The auto-start routine can be canceled at any time!
Press the Stop key [6].
Automatic restart:
The auto-start routine is restarted automatically if the routine is canceled due to one of the following
events:
• The glass door is opened,
• the outer door is opened on a device with optional gas-tight screen,
• the power supply is interrupted.
Heracell 150i/240i 50115191_C_01-2018 95 Thermo Scientific
Page 96
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.12.2. Interrupting the auto-start routine
If the STOP key in the status display is depressed (see above), the auto-start routine is interrupted
and the auto-start stop dialog box is displayed for a safety scan. The routine can now be
permanently canceled or resumed.
Figure. 40. Interrupting the auto-start routine
1. To resume the auto-start routine:
Press the BACK key [1].
The system returns to the status display, and the auto-start routine is resumed.
2. To cancel the auto-start routine:
Press the END key [2].
Heracell 150i/240i 50115191_C_01-2018 96 Thermo Scientific
Page 97
Chapter 7 Handling and control (iCanTM touchscreen controller)
The warning triangle [3] is displayed as a failure message together with the audible signal.
3. To accept the failure message:
Press any position on the display.
The ERROR dialog box with the description of the error is displayed.
4. To end the auto-start routine:
Press the END key [4].
The system returns to the main menu.
7.13. Running the contra-con routine
contra-con is an automatic decontamination routine for disinfecting the device work space. For a
detailed description of this device function (see „contra-con decontamination routine“ on page 137).
7.14. User configuration
The user configuration settings allow the user interface and the additional device functions to be
adjusted to the requirements of everyday operation. The user configuration menu is split into six
categories:
• Settings
• Event logging
•Options
• Icon description
• Keypad lock
• Software versions
To make a user-specific setting in a dialog box, navigate through the submenus listed in the
illustrations and open the dialog box.
7.14.1. Settings
The input dialogs of the SETTINGS category comprise all settings for the individual configuration of
the user display screen and the user interface:
• Keypad lock coding
• Date/time setting
• Contrast setting
• Key tone setting
• Interface configuration
• User display screen language setting
• Reminder interval setting
Heracell 150i/240i 50115191_C_01-2018 97 Thermo Scientific
Page 98
7.14.1.1. Changing the keypad lock code:
Chapter 7 Handling and control (iCanTM touchscreen controller)
Figure. 41. Changing the code
The keypad lock prevents the unauthorized changing of the operational settings. Only those keys
are locked where values can be entered.
The keypad is locked by entering four numbers.
• Upon delivery, the default code is: 0000.
This default can be changed into a user-defined code that is then enabled using the KEYPAD LOCK
dialog box (see „Enabling/disabling the keypad lock“ on page 121).
1. To enter the default 0000:
Press the corresponding numeric keys.
The number combination is displayed hidden in the input box [1].
2. To delete the input numbers:
Press the DELETE key [2].
3. To exit the menu:
Heracell 150i/240i 50115191_C_01-2018 98 Thermo Scientific
Page 99
Chapter 7 Handling and control (iCanTM touchscreen controller)
Press the BACK key [4].
4. To confirm the input:
Press the ENTER key [3].
The system changes to the CODE NEW menu.
Entering the new 4-digit code:
Press the corresponding numeric keys.
The number combination is displayed in the input box [1].
Fig.41: To set the cursor to the left to overwrite a value:
5. Press the BACKSPACE key (<<) [6].
6. To accept and save the input value:
Press the ENTER key [5].
The system returns to the Settings menu.
Note
Changing the user-defined code:
The user-defined code can be changed as often as required using the same procedure:
• Activate the recoding function by entering the valid code.
• Enter the new code and confirm it.
Heracell 150i/240i 50115191_C_01-2018 99 Thermo Scientific
Page 100
Chapter 7 Handling and control (iCanTM touchscreen controller)
7.14.1.2. Setting date/time:
The input dialog allows date and time to be set to the required time zone.
Figure. 42. Date / time setting
1. To enter the date:
Press the numeric key [2].
The input numbers are displayed in the input box [1].
2. To set the cursor to the left to overwrite a value:
Press the BACKSPACE key (<<) [4].
3. To accept and save the input value:
Press the ENTER key [3].
4. The system returns to the Date/Time menu.
5. Enter the time accordingly.
6. To accept and save the input value:
Press the ENTER key [3].
The system returns to the Date/Time menu.
Heracell 150i/240i 50115191_C_01-2018 100 Thermo Scientific
Loading...