Thermo Scientific HERAcell 150i, HERAcell 240i Operating Instructions Manual

Operating Instructions

CO2 Incubator
HERAcell

®

150i / 240i

with Decontamination Routine

50115191 A / 08.2009

Copyright 2009 Thermo Fisher Scientific. All rights reserved.

These operating instructions are protected by copyright. Rights resulting
thereof, particularly reprint, photomechanical or digital postprocessing or
reproduction, even in part, are only allowed with the written consent of the
Thermo Electron LED GmbH.
This regulation does not apply to reproductions for in-plant use.
The contents of the operating instructions are subject to change without further
notice. Concerning translations into foreign languages, the German version of
these operating instructions is binding.

Trademarks

HERAcell® and iCan™ are registered trademarks of Thermo Scientific.
Thermo Scientific is a brand of Thermo Fisher Scientific Inc.
All trademarks mentioned in the operating instructions are the exclusive property
of the respective manufacturers.

Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Germany

Thermo Electron LED GmbH is a subsidiary company of:
Thermo Fisher Scientific Inc.
81 Wyman Street
Waltham, MA 02454
USA

Operating Instructions | HERAcell® 150i / 240i

Contents

3

50115191 A / 08.2009

1 General notes 9

1.1 Identification of the device and of the documentation....................................... 10

1.2 Instruction of the operating personnel................................................................ 10

1.3 Applicability of the instructions ........................................................................... 11

1.4 Warranty ............................................................................................................... 11

1.5 Explanation of safety information and symbols ................................................. 12

1.5.1 Safety information and symbols used in the operating instructions........... 12

1.5.2 Additional symbols for safety informations: ................................................ 12

1.5.3 Symbols on the device .................................................................................. 13

1.6 Intended purpose of the device........................................................................... 14

1.6.1 Correct use ..................................................................................................... 14

1.6.2 Incorrect use................................................................................................... 14

1.7 Standards and directives ..................................................................................... 15

1.8 Safety notes on gases.......................................................................................... 16

1.8.1 Safety notes on carbon dioxide (CO

2

) .......................................................... 16

1.8.2 Safety notes on oxygen (O

2

) ......................................................................... 17

1.8.3 Safety notes on nitrogen (N

2

)........................................................................ 17

2 Delivery of the device 18

2.1 Packaging ............................................................................................................. 18

2.2 Acceptance inspection......................................................................................... 18

2.3 Standard equipment components HERAcell

®

150i............................................ 19

2.4 Optional equipment components HERAcell

®

150i............................................. 19

2.5 Standard equipment components HERAcell

®

240i............................................ 20

2.6 Optional equipment components HERAcell

®

240i............................................. 21

3 Installation of the device 22

3.1 Ambient conditions.............................................................................................. 22

3.2 Room ventilation .................................................................................................. 22

3.3 Space requirements ............................................................................................. 23

3.4 Transport .............................................................................................................. 24

3.5 Stacking ................................................................................................................ 24

3.6 Retrofitting/Modifications .................................................................................... 25

4 Description of the device 26

4.1 Front view of HERAcell® 150i.............................................................................. 26

4.2 Rear view HERAcell

®

150i ................................................................................... 28

4.3 Front view HERAcell

®

240i .................................................................................. 32

4.4 Rear view HERAcell

®

240i ................................................................................... 34

4.5 Safety devices ...................................................................................................... 38

4.6 Work space atmosphere ...................................................................................... 38

4.7 Door switch .......................................................................................................... 40

4.8 Sensor system...................................................................................................... 41

4.9 Supply interface ................................................................................................... 42

4.10 Work space components ................................................................................... 44

4.10.1 Interior container.......................................................................................... 44

4.10.2 Optional split gas-tight screens ................................................................... 44

4.10.3 Water reservoir............................................................................................. 45

4.10.4 Gas humidification (optional, only for O

2

control)...................................... 45

4.10.5 Heating system............................................................................................. 45

Operating Instructions | HERAcell® 150i / 240i

Contents

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50115191 A / 08.2009

4.10.6 Rear panel openings .................................................................................... 46

4.10.7 Shelf system................................................................................................. 47

4.10.8 Bottle turning device for HERAcell® 240i (optional) .................................. 47

4.10.9 Water pump.................................................................................................. 48

5 Start-up 49

5.1 Preparing the work space .................................................................................... 49

5.2 Installing the shelf system ................................................................................... 49

5.2.1 Installation/removal of the support rail ......................................................... 49

5.2.2 Installing the shelf supports: ......................................................................... 50

5.2.3 Levelling the device ....................................................................................... 50

5.3 Installing the gas humidification (optional, only for O

2/N2

-control)................... 50

5.4 Installing the optional bottle turning devices HERAcell

®

240i ........................... 51

5.5 Installing the optional center strut HERAcell

®

240i for split inserts................... 54

5.6 Gas connection..................................................................................................... 55

5.6.1 Installing gas pressure hoses ........................................................................ 55

5.6.2 Installing devices without the optional gas monitoring system .................. 56

5.6.3 CO

2

connection with gas monitoring system (optional) ..............................57

5.6.4 Combined CO

2/O2/N2

connection with

gas monitoring system (optional) ................................................................. 57

5.7 Power supply connection .................................................................................... 59

5.8 Connecting the RS 232 interface ......................................................................... 60

5.9 Connecting the USB interface (optional)............................................................. 60

5.10 Connecting the alarm contact............................................................................ 61

6 Operation 63

6.1 Preparing the device ............................................................................................ 63

6.2 Starting operation ................................................................................................ 64

7 Handling and control (iCan™ touchscreen controller) 65

7.1 Power switch ........................................................................................................ 65

7.2 Operating panel and operating structure............................................................ 65

7.3 Factory presettings of the iCan™ touchscreen controls..................................... 68

7.4 Heat-up phase of the control loop sensors......................................................... 68

7.5 Event actions for settings .................................................................................... 69

7.6 Setting the temperature set value ....................................................................... 70

7.7 Setting the CO

2

set value..................................................................................... 71

7.8 Setting the O

2

set value ....................................................................................... 72

7.9 Auto-start routine ................................................................................................. 73

7.9.1 Activating auto-start....................................................................................... 75

7.9.2 Interrupting the auto-start routine ................................................................. 76

7.10 Running the contra-con routine......................................................................... 76

7.11 User configuration.............................................................................................. 76

7.11.1 Settings......................................................................................................... 77

7.11.2 Event logging ............................................................................................... 83

7.11.3 Options ......................................................................................................... 85

7.11.4 Icon description............................................................................................ 90

7.11.5 Enabling/disabling the keypad lock............................................................. 92

7.11.6 Software versions ........................................................................................ 93

7.12 Scaling the trend display ................................................................................... 94

7.13 Error messages .................................................................................................. 95

Operating Instructions | HERAcell® 150i / 240i

Contents

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50115191 A / 08.2009

7.13.1 Response to an error message event ......................................................... 95

7.13.2 Troubleshooting........................................................................................... 95

8 Shut-down 99

8.1 Shutting the device down.................................................................................... 99

9 Cleaning and disinfection 100

9.1 Cleaning.............................................................................................................. 100

9.2 Decontamimation procedures ........................................................................... 101

9.3 Wipe/spray disinfection ..................................................................................... 101

9.4 contra-con decontamination routine................................................................. 104

9.4.1 Activating the contra-con routine................................................................ 107

9.4.2 Interrupting contra-con ................................................................................ 107

9.4.3 contra-con interruption due to error ........................................................... 108

9.4.4 Completing contra-con ................................................................................ 109

10 Maintenance 110

10.1 Inspections and checks.................................................................................... 110

10.2 Service intervals............................................................................................... 110

10.3 Preparing the temperature calibration ............................................................ 111

10.4 Temperature calibration procedure ................................................................ 112

10.5 Preparing the CO

2

calibration.......................................................................... 113

10.6 CO

2

calibration procedure ............................................................................... 115

10.7 Replacing the gas inlet filter............................................................................. 116

10.8 Replacing the device fuses .............................................................................. 116

10.9 Replacing the door seal ................................................................................... 117

11 Disposal 118

12 Technical data 120

13 Annex: Data communication 124

13.1 Interfaces .......................................................................................................... 124

13.1.1 RS 232 interface ......................................................................................... 124

13.1.2 USB port (optional) .................................................................................... 124

13.1.3 Installing the USB port driver .................................................................... 125

13.2 Structure of the command sequences for data communication ................... 127

13.2.1 Description of the protocol ........................................................................ 127

13.3 Overview of general parameters (addresses 0xxx)........................................ 128

13.3.1 Reading general parameters ..................................................................... 128

13.4 Overview of incubator parameters (addresses 2xxx)..................................... 129

13.4.1 Reading basic parameters ......................................................................... 129

13.4.2 Reading internal function parameters....................................................... 130

13.5 Error memory structure ................................................................................... 132

13.5.1 Error memory data set structure scheme:................................................ 133

13.5.2 Overview of the possible error messages in hex coding......................... 133

Operating Instructions | HERAcell® 150i / 240i

Contents

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50115191 A / 08.2009

13.5.3 Overview of the possible error messages in bit coding........................... 134

13.6 Data logger structure ....................................................................................... 136

13.6.1 Data logger data set structure scheme:.................................................... 137

13.6.2 Overview of possible event entries in bit coding ..................................... 138

13.7 Examples of data logger codes ....................................................................... 140

13.7.1 Functions for data logger queries ............................................................. 140

13.7.2 Example of a code for a a data logger query............................................ 140

13.8 Program Heracell 150i & 240i........................................................................... 145

13.8.1 Installing the Heracell 150i & 240i.............................................................. 146

13.8.2 Operating the Heracell 150i & 240i ............................................................ 147

14 Device log 154

15 Certificate of decontamination 155

Betriebsanleitung | HERAcell® 150i / 240i

Figures

7

50115191 A / 08.2009

Fig. 1: Device dimensions.................................................................................... 23

Fig. 2: Lift points................................................................................................... 24

Fig. 3: Stacking ..................................................................................................... 24

Fig. 4: Front view HERAcell

®

150i ....................................................................... 27

Fig. 5: Rear view HERAcell

®

150i ........................................................................ 29

Fig. 6: Looped-through gas supply HERAcell

®

150i........................................... 31

Fig. 7: Front view HERAcell

®

240i ....................................................................... 33

Fig. 8: Rear view HERAcell

®

240i ........................................................................ 35

Fig. 9: Looped-through gas supply HERAcell

®

240i........................................... 37

Fig. 10: Door switch ............................................................................................... 40

Fig. 11: Temperature, CO

2

,and O2 sensors .......................................................... 41

Fig. 12: Supply interface ........................................................................................ 42

Fig. 13: Work space components .......................................................................... 44

Fig. 14: Water reservoir.......................................................................................... 45

Fig. 15: Rear panel openings ................................................................................. 46

Fig. 16: Bottle turning device................................................................................. 47

Fig. 17: Water pump............................................................................................... 48

Fig. 18: Support rail installation/removal .............................................................. 49

Fig. 19: Shelf support installation .......................................................................... 50

Fig. 20: Installing gas humidification ..................................................................... 50

Fig. 21: Installing the bottle turning devices ......................................................... 51

Fig. 22: Bottle turning speed.................................................................................. 53

Fig. 23: Center strut installation............................................................................. 54

Fig. 24: Installing gas pressure hoses ................................................................... 55

Fig. 25: Gas connection without gas monitoring system..................................... 56

Fig. 26: Gas connection with gas monitoring system (optional) ......................... 57

Fig. 27: Combined connection with gas monitoring system (optional)............... 58

Fig. 28: Combined gas connection with gas monitoring system......................... 59

Fig. 28: Power supply connection ......................................................................... 59

Fig. 29: Connection example ................................................................................. 62

Fig. 30: Power switch ............................................................................................. 65

Fig. 31: Pressure-sensitive areas ........................................................................... 65

Fig. 32: iCan™ touchscreen withoutO

2/N2

gas supply ..........................................66

Fig. 33: iCan™ touchscreen with combined gas connection ............................... 66

Fig. 34: Menu structure overview.......................................................................... 67

Fig. 35: Heat-up phase display .............................................................................. 68

Fig. 36: Setting the temperature set value ............................................................ 70

Fig. 37: Setting the CO

2

set value.......................................................................... 71

Fig. 38: Setting the O

2

set value ............................................................................ 72

Fig. 39: Activating the auto-start routine............................................................... 75

Fig. 40: Interrupting the auto-start routine............................................................ 76

Fig. 41: Changing the code .................................................................................... 77

Fig. 42: Date / time setting ..................................................................................... 78

Fig. 43: Display contrast setting ............................................................................ 79

Fig. 44: Key tone setting ........................................................................................ 79

Fig. 45: RS 232 interface baud rate setting ........................................................... 80

Fig. 46: USB interface baud rate setting................................................................ 80

Fig. 47: Language setting....................................................................................... 81

Fig. 48: Reminder interval setting.......................................................................... 82

Fig. 49: Event display ............................................................................................. 83

Betriebsanleitung | HERAcell® 150i / 240i

Figures

8

50115191 A / 08.2009

Fig. 50: Logging cycle setting................................................................................ 83

Fig. 51: Error table display ..................................................................................... 84

Fig. 52: Alarm relay setting.................................................................................... 85

Fig. 53: Low humidity setting ................................................................................ 86

Fig. 54: Gas tight screen setting ............................................................................ 87

Fig. 55: Water level setting .................................................................................... 87

Fig. 56: Alarm relay setting.................................................................................... 88

Fig. 57: Bottle turning device speed setting.......................................................... 88

Fig. 58: Switching the O

2

control on and off......................................................... 89

Fig. 59: Icon description overview ........................................................................ 90

Fig. 60: Gas monitoring icons................................................................................ 91

Fig. 61: Enabling/disabling the keypad lock.......................................................... 92

Fig. 62: Software versions ..................................................................................... 93

Fig. 63: Trend display scaling ................................................................................ 94

Fig. 64: Event error messages ............................................................................... 95

Fig. 65: Decontamination routine phases ........................................................... 106

Fig. 66: Activating contra-con.............................................................................. 107

Fig. 67: Interrupting / cancelling contra-con ....................................................... 108

Fig. 68: contra-con completion............................................................................ 109

Fig. 69: Preparing the temperature calibration ................................................... 111

Fig. 70: Temperature calibration procedure ....................................................... 112

Fig. 71: Preparing CO

2

calibration ....................................................................... 113

Fig. 72: Gas-tight screen measurement opening............................................... 114

Fig. 73: CO

2

calibration procedure ...................................................................... 115

Fig. 74: Gas inlet filter installation........................................................................ 116

Fig. 75: Device fuse replacement ........................................................................ 116

Fig. 76: Magnetic door seal replacement............................................................ 117

Operating Instructions | HERAcell® 150i / 240i

9

50115191 A / 08.2009

1 General notes

Overview of the Thermo Fisher international sales organisations

Europe, Middle East, Africa Postal address Germany:

Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold

Enquiries from Germany:

Phone Sales 0800 1 536376
Phone Service 0800 1 112110
Fax Sales/Service 0800 1 112114
E-Mail [e-mail]

Enquiries from Europe, Middle East, and Africa:

Phone + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-6772
E-Mail [e-mail]

America Postal address USA:

Thermo Fisher Scientific
275 Aiken Road
Asheville, NC 28804
USA

Enquiries from North America:

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Enquiries from Latin America:

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Enquiries from Asia Pacific:

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

10

50115191 A / 08.2009

1.1 Identification of the device and of the documentation

Identification of the device

Identfication data Device name: CO

2

incubator

Type designation: HERAcell

®

150i

HERAcell

®

240i

Serial No.: 40830469 or higher

Allocation of product documentation

User information: Operating instructions 50113925 D
Valid: 08.2009

Certifications and quality audit:

Certification: CE Certification
Test mark: GS by VDE Germany

CSA/UL by VDE Germany

1.2 Instruction of the operating personnel

These operating instructions describe the CO2 incubator HERAcell® 150i /
240i. The CO

2

incubator has been manufactured in keeping with the latest
technological developments and is operationally safe. However, the device
may present potential hazards, particularly if it is operated by inadequately
trained personnel or if it is not used in accordance with the intended
purpose. Therefore, the following must be observed to prevent accidents:

Target group •The CO

2

incubator must be operated only by trained and authorized

personnel.

Process procedures for personnel • For any operation of this device, the operator must prepare clear and

concise written instructions in the language of the operating and
cleaning personnel based on these operating instructions, applicable
safety data sheets, plant hygiene guidelines, and technical regulations,
in particular:

– which decontamination measures are to be applied for the CO

2

incubator and accessories,
– which protective measures apply when specific agents are used,
– which measures are to be taken in the case of an accident.

• Repairs to the device must be carried out only by trained and
authorized expert personnel.

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

11

50115191 A / 08.2009

1.3 Applicability of the instructions

Original document for translation • The contents of the operating instructions are subject to change

without further notice.

• Concerning translations into foreign languages, the German version of
these operating instructions is binding.

• Keep these operating instructions close to the device so that safety
instructions and important information are always accessible.

Should you encounter problems that are not detailed adequately in these
operating instructions, please contact Thermo Scientific immediately for
your own safety.

1.4 Warranty

Requirements for warranty Thermo Scientific warrants the operational safety and functions of the CO

2

incubator only under the condition that:

• the device is operated and serviced exclusively in accordance with its
intended purpose and as described in these operating instructions,

• the device is not modified,

• only original spare parts and accessories that have been approved by
Thermo Fisher Scientific are used,

• inspections and maintenance are performed at the specified intervals.

The warranty is valid from the date of delivery of the device to the operator.

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

12

50115191 A / 08.2009

1.5 Explanation of safety information and symbols

1.5.1 Safety information and symbols used in the operating

instructions

WARNING: Personal injuries

Indicates a hazardous situation which, if not avoided, could
result in death or serious injuries.

CAUTION: Personal injuries

Indicates a hazardous situation which, if not avoided, could
result in minor or moderate injuries.

NOTICE: Property damage

Indicates a situation which, if not avoided, could result in
property damage.

NOTE: Useful information

Is used for applicational hints and useful information.

1.5.2 Additional symbols for safety informations:

Wear safety gloves!

Wear safety goggles!

Harmful liquids!

Electric shock!

Hot surfaces!

Fire hazard!

Explosion hazard!

Suffocation hazard!

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

13

50115191 A / 08.2009

1.5.3 Symbols on the device

CE conformity mark: confirms conformity according to EU Guidelines

VDE test mark

Mark of conformity USA/Canada

Observe operating instructions!

H

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

14

50115191 A / 08.2009

1.6 Intended purpose of the device

1.6.1 Correct use

Use The CO

2

incubator HERAcell® 150i / 240i is a laboratory device for
preparing and cultivating cell and tissue cultures. The device allows the
simulation of the special physiological ambient conditions for these
cultures due to the exact control of:

• temperature,

•CO

2

content,

•O

2/N2

content,

• and increased relative humidity.

Fields of application HERAcell

®

150i / 240i has been designed for installation and operation in

the following fields of application:

• Laboratories for cytobiological and biotechnological experiments of
safety levels L1, L2, and L3.

• Medical-microbiological laboratories in accordance with DIN 58 956.

• Laboratories in the central area of clinics and hospitals.

Gas supply system The gases required for the incubator (CO

2

and / or O2/N2) are supplied to
the device from a separate gas supply system, either from gas cylinders or
from a central pressurized gas container.
The layout of the gas supply system must ensure that the operating
pressure of the gas supply lines can be set to a range between 0.8 bar
(min.) to 1 bar (max.) and that the pressure cannot be changed.
If the device is equipped with the optional gas monitoring system, up to
four devices can be interconnected in line, independent of the of the gas
supply system capacity.
The CO

2

incubator is suitable for continuous operation.

1.6.2 Incorrect use

Incorrect use Do not use cell or tissue cultures in the device that are not in accordance

with the regulations of safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:

• are easily ignitible or explosive,

• release vapors that form combustible or explosive mixtures when
exposed to air,

• release poisons.

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

15

50115191 A / 08.2009

1.7 Standards and directives

The device complies with the following standards and guidelines:

• DIN EN 61010 - 1: 2002, DIN EN 61010 - 2 - 010 : 2004

• Low Voltage Guideline 2006/95/EG

• EMC Guideline 2004/108/EG

• UVV BGV D4 Revised Version
(UVV VBG 20, continues to be valid for old stock)

The following safety regulations must be observed if the device is operated
within the territory of the Federal Republic of Germany:

•BGR 104

•BGR 120

•BGI 629

•BGI 630

• TRG 280

• EC Official Gazette, L 374

• Safety data sheets of the gas suppliers with regard to the particular
characteristics of CO

2

, O2, and N2.

• Principles of good microbiological proceedings, notice of the trade
association of the German chemical industry.

For other countries, the applicable national regulations are binding.

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

16

50115191 A / 08.2009

1.8 Safety notes on gases

Installation work:

Any work to supply lines and pressurized gas containers,
cylinders or containers used for storing CO

2

or O2/N2 must only

be carried out by expert personnel using the appropriate tools.

Instruction of the personnel:

Personnel operating devices with CO

2

supply must be

instructed about the particularities in the handling of CO

2

before starting their work:

• Correct operation of pressurized gas containers and gas
supply systems,

• obligation to report damages and shortcomings in CO

2

supply lines,

• measures to be taken in case of accidents or failures.

These instructions must be repeated at appropriate intervals and must
comprise the particular operating instructions of the gas supplier.

1.8.1 Safety notes on

carbon dioxide (CO

2

)

Carbon dioxide Since CO

2

is rated as a harmful gas, certain safety instructions must be

observed when the CO

2

incubator is started up and when the device is

operated.

Suffocation hazard!

Large amounts of CO

2

released into the room atmosphere may
cause suffocation.
If CO

2

is released, initiate safety measures immediately!

• Leave the room immediately and do not allow others to

enter the room!

• Inform security service or fire department!

Operating Instructions | HERAcell® 150i / 240i

1 General notes1 General notes

17

50115191 A / 08.2009

1.8.2 Safety notes on oxygen (O2)

Oxygen O

2

is a gas that promotes combustion and may explode in combination

with grease-containing materials.

Oxygen explosion!

O

2

may explode in combination with oils, greases, and
lubricants. If highly compressed oxygen comes in contact with
grease- or oil-containing substances, the mixture may explode!

• For cleaning these device components, use only oil- and

grease-free lubricants.

• Keep all connections and components of the oxygen system

free from substances that contain oil, grease, or lubricant!

Fire hazard!

Released oxygen promotes combustion. Do not use open
flames in the vicinity of oxygen-operated systems!

• Do not smoke in the vicinity of oxygen systems.

• Do not expose the components of an oxygen system to

excessive heat.

1.8.3 Safety notes on nitrogen (N

2

)

Nitrogen Nitrogen mixes easily with air. High concentrations of nitrogen reduce the

oxygen content in the air.

Suffocation hazard!

Large amounts of N

2

released into the room atmosphere may
cause suffocation.
If N

2

is released, initiate safety measures immediately!

• Leave the room immediately and do not allow others to

enter the room!

• Inform security service or fire department!

Operating Instructions | HERAcell® 150i / 240i

18

50115191 A / 08.2009

2 Delivery of the device

2.1 Packaging

The CO2 incubator HERAcell® 150i / 240i is delivered in a stable packaging
box. All packaging materials can be separated and are reusable:

Packaging materials •Packaging carton:

Recycled paper

• Foam elements:
Styrofoam (CFC-free)

•Pallet:
Untreated wood

•Packaging film:
Polyethylene

• Packaging ribbons:
Polypropylene

2.2 Acceptance inspection

After the device has been delivered, check the delivery immediately for:

• completeness,

• possible damage.

Damage report If components are missing or damage is found on the device or the

packaging, esp. damages caused by humidity and water, please contact
the linehauler as well as the Technical Support of Thermo Scientific
immediately.

Operating Instructions | HERAcell® 150i / 240i

2 Delivery of the device2 Delivery of the device2 Delivery of the device

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50115191 A / 08.2009

2.3 Standard equipment components HERAcell® 150i

2.4 Optional equipment components HERAcell® 150i

*1) O2 sensor with gas humidification set is packed in a separate carton and placed into

the work space during transport.

Solid glass door and
continuous shelves

3-door gas tight screen and
continuous shelves

Shelf 3 3

Support rail for shelf 4 4

Shelf supports for shelf 6 6

Insert for pressure compensation opening 1 1

Plug for pipe channel 1 1

Power supply cable 1 1

Connector, potential-free contact 1 1

Spare caps, set 1 1

CO

2

connecting hose set 1 1

Open-end wrench, 24 mm 1 1

Allen wrench 2 mm for blower wheel 1 1

Allen wrench 3 mm for blower wheel cover 1 1

Operating instructions (CD) 1 1

Water pump 1 1

Quantity of the delivered components
(in piece)

HERAcell

®

150i

CO

2

incubator or CO2/O2 incuba tor

Solid glass door and
continuous shelves

3-door gas tight screen and
continuous shelves

O2 connecting hose set

11

O2 sens or wit h set for gas humidific ation *1)

11

CO2 connec ting hos e set, gas monitoring (optional)

11

O2 / N2 connec ting hos e set, gas monitoring (optional)

11

Quantity of the delivered components
(in piece)

HERAcell

®

150i

CO

2

incubator or CO2/O2 incuba tor

Operating Instructions | HERAcell® 150i / 240i

2 Delivery of the device2 Delivery of the device2 Delivery of the device

20

50115191 A / 08.2009

2.5 Standard equipment components HERAcell® 240i

Solid glass door and
continuous shelves
(standard version)

Solid glass door
and split shelves
(optional)

6-door gas tight
screen and
continuous shelves
(standard version)

6-door gas tight
screen and split
shelves
(optional)

Shelf 3 6 3 6

Support rail for shelf 4 6 4 6

Shelf supports for shelf 6 12 6 12

Insert for pre ssure
compensation opening

111 1

Plug for pipe channel 1 1 1 1

Power supply cable 1 1 1 1

Connector, potential-free
contact

111 1

Spare caps, set 1 1 1 1

CO

2

connecting hose set 1 1 1 1

Open-end wrench, 24 mm 1 1 1 1

Allen wrench 2 mm for
blower wheel

111 1

Allen wrench 3 mm for
blower wheel cover

111 1

Operating instructions (CD) 1 1 1 1

Water pump 1 1 1 1

Quantity of the delivered
components
(in piece)

HERAcell

®

240i

CO

2

incuba tor or CO2/O2 incubator

Operating Instructions | HERAcell® 150i / 240i

2 Delivery of the device2 Delivery of the device2 Delivery of the device

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50115191 A / 08.2009

2.6 Optional equipment components HERAcell® 240i

*1) O2 sensor with gas humidification set is packed in a separate box that is placed into

the work space during transport.
*2) For bottle turning devices, the version with split shelves is not available.
*3) Each level of a bottle turning device is packed in a separate box. The boxes (1 to 4)

are delivered with the device.

Solid glass door and
continuous shelves
(standard version)

Solid glass door
and split shelves
(optional)

6-door gas tight
screen and
continuous shelves
(standard version)

6-door gas tight
screen and split
shelves
(optional)

O

2

connecting

hose set

111 1

O

2

gas humidification
system connecting hose
set *1)

111 1

Bottle turning device
(motor with four
rollers, optional) *2), *3 )

1—1 —

CO

2

connecting hose set,

gas monitoring (optional)

111 1

O

2

/ N2 connecting hose set,
gas monitoring (optional)

111 1

Quantity of the delivered
components
(in piece)

HERAcell

®

240i

CO

2

incuba tor or CO2/O2 incubator

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

3 Installation of the device

3.1 Ambient conditions

The device must only be operated at locations that meet the particular
ambient conditions listed below:

Requirements:

Location • Draft-free and dry location.

• The minimal distance to adjacent surfaces must be observed on all
sides (see Section 3.3.).

• The operating room must be equipped with appropriate room
ventilation.

• Solid, level, fire-proof surface.

• Vibration-proof substructure (floor stand, lab table) capable of bearing
the weight of the device and of accessories (particularly if several
devices are stacked).

• The electrical system of the device has been designed for an operating
height of up to 2000 m above sea level.

• To ensure a constant incubation temperature of 37 °C, the ambient
temperature must be within a range of +18 °C to +33 °C.

• Relative humidity up to 80% (max.).

• No direct exposure to sunlight.

• Devices that produce excessive heat must not be placed near the
location of the HERAcell

®

150i / 240i.

3.2 Room ventilation

When CO2/O2/N2 is supplied, the work space of the incubator is slightly
pressurized. The pressure is released through the pressure compensation
opening into the operating room.
Pressure compensation and any opening of the glass door/gas-tight screen
during the operation of the device will release very small quantities of CO

2

/

O

2/N2

into the operating room; the room ventilation must be capable of
carrying the released gas safely off into the open.
In addition, heat dissipating from the device during continuous operation
may cause a change in the room climate.

• Therefore, the HERAcell

®

150i / 240i must only be installed in rooms

with sufficient ventilation.

• Do not install the device in room recesses without ventilation.

Technical ventilation • The room ventilation should be a technical ventilation that complies

with the requirements of BGR 120 (Guidelines for laboritories,
Germany) or some other suited ventilation system with appropriate
capacity.

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

3.3 Space requirements

Fig. 1: When installing the device, make sure that the

installation and supply connections are freely
accessible.
The control box on the rear panel of the device may
serve as a spacer to adjacent objects. The side
distances given are minimal distances.
To protect the CO

2

incubator against contamination,
use a floor stand even if the device is installed near the
floor. The height of the floor stand should not fall
below 200 mm.

Accessibility of the devices:

To ensure the accessibility for care and
maintenance works, keep larger side and
rear distances.

Fig. 1: Device dimensions

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

3.4 Transport

Fig. 2: For transport, do not lift the device using the

doors or components attached to the device (e.g.
control box on rear panel) as lift points.

Lift points:

Lift the device only using the lift points.

3.5 Stacking

Fig. 3: HERAcell® 150i / 240i is suited for stacking up

to two devices of the same device type. For thermal
separation, an adapter plate [3] can be placed between
the two devices.

HERAcell

®

150i

• Place the adapter plate with the receptacles [4] at
the bottom onto the stacking elements [5] on top
of the device. Make sure that the adapter plate is
seated safely on the stacking elements.

• Place the device to be stacked with the device
stands [1] onto the stacking elements [2] at the top
of the adapter plate.

HERAcell

®

240i

• Place the adapter plate with the receptacle
recesses [8] at the bottom onto the stacking
elements [9] on top of the device. Make sure that
the adapter plate is seated safely on the recesses.

• Place the device to be stacked with the device
stands [6] onto the stacking elements [7] at the top
of the adapter plate.

The devices are secured to one another by their own
weight.

Transporting stacked devices!

The stacking elements are not locking
elements. Therefore, it is not permitted to
transport stacked devices.

Fig. 2: Lift points

Fig. 3: Stacking

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50115191 A / 08.2009

Mobile racks Fastening on mobile racks:

If the devices are installed onto mobile racks, make sure that
the rollers [10] are secured with a locking brake during the
operation of the incubators and that the rollers are oriented
toward the front for increased stability.

Condensate formation during the operation of stacked devices:

If stacked devices of type HERAcell

®

150i are operated at an
ambient temperature of more than 26 °C, condensate formation
may occur on the upper device while the contra-con
decontamination routine is run for the lower device.
To prevent condensate formation, an adapter plate should be
placed between the two devices for thermal separation.

3.6 Retrofitting/Modifications

Retrofitting options The following components can be retrofitted to the standard version:

HERAcell

®

150i

• 3-segment gas-tight screen (the gas-tight screen is installed in place of
the glass door),

• door hinge replacement on outer door and glass door (RH / LH door
opening),

• lockable outer door,

• split inserts,

• infrared (IR) sensor,

• PCB for USB interface.

HERAcell

®

240i

• 6-segment gas-tight screen (the gas-tight screen is installed in place of
the glass door),

• door hinge replacement on outer door and glass door,

• lockable outer door,

• split inserts,

• infrared (IR) sensor,

• PCB for USB interface.

Modifications:

Retrofittings and modifications must only be performed by the
Technical Service of Thermo Electron LED.

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

4 Description of the device

4.1 Front view of HERAcell® 150i

Front view [1] Stacking elements

[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Oxygen sensor (optional)
[7] Pressure compensation opening with insert
[8] Measuring opening

[9] Outer door
[10] Outer door seal, replaceable
[11] Stand, height-adjustable
[12] Gas humidification (optional)
[13] Water level sensor
[14] Nameplate
[15] Power switch
[16] Support rail
[17] Shelf
[18] Latch, glass door
[19] Support hook for shelf
[20] Access port with plug
[21] Glass door seal, replaceable
[22] iCan™ Touchscreen (Operating panel)
[23] Door handle

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50115191 A / 08.2009

Fig. 4: Front view HERAcell® 150i

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

4.2 Rear view HERAcell® 150i

Rear view [1] Access port, Ø 42 mm

[2] Pressure compensation opening

[3] Control box with supply interface for combined gas connection

(optional) CO

2

and O2/N2 without gas monitoring
[4] Condensate drain gutter
[5] Diagram: Gas connection CO

2

and O2/N2 without gas monitoring

[6] O

2/N2

gas cylinder

[7] CO

2

gas cylinder

[8] Secondary CO

2

gas supply B with optional gas monitoring

[9] Primary CO

2

gas supply A with optional gas monitoring

[10] Secondary O

2/N2

gas supply B with optional gas monitoring

[11] Primary O

2/N2

gas supply A with optional gas monitoring

[12] Control box with supply interface for combined gas connection

(optional) CO

2

and O2/N2 with gas monitoring (optional)

[13] Distributor connection for looping through the CO

2

gas supply for

up to three additional devices (independent of device type)

[14] Distributor connection for looping through the O

2/N2

gas supply for

up to 3 additional devices (independent of device type)

[15] Diagram: Gas connection CO

2

and O2/N2 with optional gas

monitoring

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Fig. 5: Rear view HERAcell® 150i

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Looped-through gas supply for HERAcell® 150i:

[1] Control box with supply interface for combined gas connection

(optional) CO

2

and O2/N2 with gas monitoring (optional)

[2] Distributor connection for looping through the CO

2

gas supply for up

to three additional devices (independent of device type)

[3] Distributor connection for looping through the O

2/N2

gas supply for

up to 3 additional devices (independent of device type)
[4] Tee joint for fitting the gas pressure hoses together
[5] Gas pressure hose for looping-through the gas supply
[6] Diagram: Gas connection CO

2

and O2/N2 with optional gas

monitoring
[7] HERAcell

®

150i with looped-through gas supply; therefore device

must only be equipped with combined gas connection (optional) CO

2

and O

2/N2

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Fig. 6: Looped-through gas supply

HERAcell® 150i

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

4.3 Front view HERAcell® 240i

Front view [1] Stacking elements

[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Oxygen sensor (optional)
[7] Receptacle for bottle turning device (optional)
[8] Measuring opening
[9] Plug for bottle turning device (optional)

[10] Outer door
[11] Outer door seal, replaceable
[12] Stand, height-adjustable
[13] Drive roller for bottle turning device (optional)
[14] Gas humidification (optional)
[15] Water level sensor
[16] Insert for bottle turning device (optional)
[17] Nameplate
[18] Cylinders (optional)
[19] Power switch
[20] Support rail
[21] Shelf
[22] Latch, glass door
[23] Support hook for shelf
[24] Pressure compensation opening with insert
[25] Access port with plug
[26] Glass door seal, replaceable
[27] iCan™ Touchscreen (Operating panel)
[28] Door handle

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Fig. 7: Front view HERAcell® 240i

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

4.4 Rear view HERAcell® 240i

Rear view [1] Pressure compensation opening

[2] Access port, Ø 42 mm
[3] Condensate drain gutter
[4] Control box with supply interface for combined gas connection

(optional) CO

2

and O2/N2 without gas monitoring

[5] Diagram: Gas connection CO

2

and O2/N2 without gas monitoring

[6] O

2/N2

gas cylinder

[7] CO

2

gas cylinder

[8] Secondary CO

2

gas supply B with optional gas monitoring

[9] Primary CO

2

gas supply A with optional gas monitoring

[10] Secondary O

2/N2

gas supply B with optional gas monitoring

[11] Primary O

2/N2

gas supply A with optional gas monitoring

[12] Control box with supply interface for combined gas connection

(optional) CO

2

and O2/N2 with gas monitoring (optional)

[13] Distributor connection for looping through the CO

2

gas supply for

up to three additional devices (independent of device type)

[14] Distributor connection for looping through the O

2/N2

gas supply for

up to 3 additional devices (independent of device type)

[15] Diagram: Gas connection CO

2

and O2/N2 with optional gas

monitoring

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Fig. 8: Rear view HERAcell® 240i

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Looped-through gas supply for HERAcell® 240i:

[1] Control box with supply interface for combined gas connection

(optional) CO

2

and O2/N2 with gas monitoring (optional)

[2] Distributor connection for looping through the CO

2

gas supply for up

to three additional devices (independent of device type)
[3] Distributor connection for looping through the O

2/N2

gas supply for

up to 3 additional devices (independent of device type)
[4] Tee joint for fitting the gas pressure hoses together
[5] Gas pressure hose for looping-through the gas supply
[6] Diagram: Gas connection CO

2

and O2/N2 with optional gas

monitoring
[7] HERAcell

®

240i with looped-through gas supply; therefore device

must only be equipped with combined gas connection (optional) CO

2

and O

2/N2

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

Fig. 9: Looped-through gas supply

HERAcell® 240i

Operating Instructions | HERAcell® 150i / 240i

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50115191 A / 08.2009

4.5 Safety devices

The device is equipped with the following safety devices:

• A door switch interrupts the CO

2/O2/N2

supply and the work space

heating when the glass door is opened.

• The optional gas detector switches the gas supply over to a full gas
cylinder.

• An independent thermal protection protects the samples from harmful
overheating in case of failures.

• A pressure compensation opening ensures pressure compensation in
the device work space.

• The alarm relay circuit uses audible and visual alarms to indicate errors
during operation.

4.6 Work space atmosphere

In the work space of the incubator, the particular physiological ambient
conditions for the preparation and cultivation of cell and tissue cultures are
simulated. The work space atmosphere is determined by the following
factors:

• Temperature

• Relative humidity

•CO

2

concentration

•O

2

concentration (optional)

Temperature:

Temperature conditions To ensure undisturbed operation, the temperature in the operating room

must be at least 18 °C and the incubation temperature must exceed this
temperature by at least 3 °C.
The heating system controls the incubation temperature from this
temperature threshold up to 55 °C. The principle of air jacket heating and
the additional, separate heating of the outer door and glass door/gas-tight
screen minimize the risk of condensate formation at the side walls, at the
ceiling of the work space, and at the glass door/gas-tight screen.

Relative humidity:

Water quality The heating of the work space promotes the condensation of the water,

thereby ensuring a constant humidity within the work space. For the
running operation, always keep a sufficient quantity of processed water of
the following quality available:

•HERAcell

®

240i: quantity 4.5 l,

•HERAcell

®

150i: quantity 3.0 l,

Water quality recommendation:

For trouble-free operation of the equipment, fill the water reservoir with
sterilized destilled water or equivalent. The acceptable conductivity should
be within the range of 1 to 20 μS (resistivity within the range of 50 kΩ to
1 MΩ).

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50115191 A / 08.2009

Termination of warranty!

Using chlorinated tap water or additives that contain chlorine
will void the manufacture warranty. Similarly, the use of
ultrapure water whose conductivity is out of the range of 1 to
20 μS (and whose resistivity is out of the range of 50 kΩ to
1 MΩ) will void the manufacture warranty.
If you should have any questions, please contact Technical
Support of Thermo Fisher Scientific.

Under normal operating conditions and at the usual incubation
temperature of 37 °C, a constant relative humidity of approx 93% is
achieved in the work space.
If dew formation occurs on the culture containers due to
elevated relative humidity, the humidity in the work space can be adapted
to a lower value by enabling the Low Humidity feature. The relative
humidity in the work space is lowered from approx. 93% to approx. 90%.
The modification requires an extended adaption phase. To ensure that it
effectively prevents
dew formation on culture containers, it must be used as a permanent
setting.

CO

2

supply:

CO2-Work space atmosphere control To ensure the growth conditions for the cell and tissue cultures, the work

space is supplied with CO

2

.
The pH of the bicarbonate-buffered culture media largely depends on the
CO

2

content of the work space atmosphere.

The CO

2

content of the work space atmosphere can be controlled within a
range of 0 to 20%.
The supplied CO

2

must have one of the following quality characteristics:

• Purity 99.5% min.,

• medical gas quality.

O

2

supply:

O2-Work space atmosphere control If the CO

2

incubator is to be operated with more than 21% oxygen, the

work space is supplied with oxygen. The O

2

content of the work space

atmosphere can be controlled within a range of 0 to 21%.

N

2

supply:

N2-Work space atmosphere control If the oxygen content during operation is to be lowered to less than 21%

(air oxygen content), the work space is supplied with nitrogen. The O

2

content of the work space atmosphere can be controlled depending on the
configuration of the sensor within a range of 1% to 21% and 5% to 90%.

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4.7 Door switch

A door switch [1] is installed at the upper edge of the
work space opening. If the door switch is activated by
opening the glass door, the gas supply and the heating
of the work space are interrupted and the display
shows a corresponding message.
If the door remains open for more than 30 seconds, a
short audible alarm sounds. If the door remains open
for more than 10 minutes, an audible alarm sounds
and the alarm relay responds.

The outer door can only be closed after the glass door
has been latched properly.

Versions with gas-tight screen:

For devices with the optional gas-tight
screen, the door switch function described
above is activated when the outer door is
opened.

Fig. 10: Door switch

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50115191 A / 08.2009

4.8 Sensor system

The blower wheel and two sensor modules are
integral to the baseplate [1] of the measuring cell:

• Sensor [2] for the acquisition of the work space
temperature and the thermal protection,

•CO

2

sensor [3] for the acquisition of the CO2

content in the work space atmosphere.

The O

2

sensor (optional) [4] for the acquisition of the

O

2

content in the work space atmosphere is installed

at various locations next to the measuring cell:

•HERAcell

®

150i in the sidewall in the upper area [5],

•HERAcell

®

240i in the ceiling of the work space [4].

The sensor for the acquisition of the work space
temperature as well as the CO

2

sensor and the O2
sensor are integral to the control system of the device.
Their measured values are compared to the selected
set values. Based on this data, the control system
controls heating and CO

2/O2/N2

supply.
The blower intermixes the supplied gases and ensures
an even temperature distribution within the work
space.

The thermal protection has been preprogrammed at
the factory and cannot be changed. It protects the
stored cultures from overheating.
If the set temperature is exceeded by more than 1°C,
the thermal protection responds and the work space
temperature is automatically reduced to the selected
set value so that the incubation process can be
continued even in case of a failure. Any response of
the thermal protection will simultaneously trigger a
visual alarm.
If the thermal protection is enabled:

• an error message is issued,

• the alarm relay responds.
If the error message is accepted, the display shows the
Overtemperature icon to indicate the response of the
thermal protection, and the temperature display is
highlighted in red.

Fig. 11: Temperature, CO

2

,and

O

2

sensors

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50115191 A / 08.2009

4.9 Supply interface

All supply connections are installed in the supply
interface (control box [1]) in the rear of the device.

Gas connection:

The gas supply lines between the device and the gas
supply system are connected using the supplied
connecting hoses. O

2/N2

is supplied to the device

through connecting sleeve [2]. CO

2

is connected to
sleeve [3].
All process gases must be supplied to the device at a
fixed pressure that has been preset within a range of

0.8 to 1.0 bar and must remain unchanged.
Before the gases are fed into the work space, they flow
through a gas inlet filter with a separation rate of 99.97
% for a particle size of 0.3 μm (HEPA filter quality).
The illustration shows the optional combined gas
connection without the optional gas monitoring
system.

Label:

The label [4] contains information about gas supply,
an alarm contact terminal legend, and notes about the
electrical fusing of the device.

RS 232 interface:

Via the RS 232 interface [5], the incubator can be
connected to the serial interface of a PC. This
connection allows the computer-aided acquisition and
documentation of the major operating parameters
(temperature, CO

2/O2/N2

concentration, error codes,

etc.).

USB interface (optional):

As an alternative to the RS 232 interface [5], the
incubator can be connected to a PC via the optional
USB interface [6]. This connection - USB 1.1 /
USB 2.0 full speed compatible - allows the computer­aided acquisition and documentation of the major
operating parameters (temperature, CO

2/O2/N2

concentration, error codes, etc.).

Fig. 12: Supply interface

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50115191 A / 08.2009

Alarm contact:

External alarm system Fig. 12: The device can be connected to an on-site, external alarm system

(e.g. telephone system, building monitoring system, visual or audible
alarm system).
For this purpose, a potential-free alarm contact [7] is preinstalled in the
device.

Alarm contact:

The alarm contact responds for all errors reported by the
control loops (see chap. 7.13‚ pg. 95).

Power supply connection:

Fig. 12: The connection to the power supply system is
established by connecting a cable with plug for IEC connector
[9] to the socket [8]. The receptacle for the two device fuses is
integral to the socket.

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4.10 Work space components

The work space of the incubator has only a minimum
of surface, thereby supporting both the prevention of
contamination and easy, effective decontamination.

4.10.1 Interior container

All components of the work space are made of
stainless steel and have a high-gloss polished,
absolutely smooth and easy-to-clean surface. Any
embossings have a large radius.
As an option, the interior container, the shelf system,
and the blower wheel with its cover can be made of
copper material.

Oxidation of copper components:

When exposed to heat and humid air, the
copper material of the interior container
will oxidize. This results in a discoloration
of the copper components during the test
run for checking the device functions.
Do not remove the oxide layer during
routine cleaning as the antimicrobial effect
of the copper material is based on it.

The components of the shelf system can be removed
easily so that only the easily treatable, surface­reduced interior container [1] remains in the work
space for cleaning and manual disinfection works.

4.10.2 Optional split gas-tight screens

Equipment versions:

•HERAcell

®

150i with 3-segment gas-tight screen

[2],

•HERAcell

®

240i with 6-segment gas-tight screen

[3].

For devices that are equipped with the optional gas­tight
screen, the contamination hazard is considerably
lower and the recovery times of the incubation
parameters are shorter for:

• work space temperature,

•CO

2

concentration,

•O

2/N2

concentration,

• relative humidity.

Advantage of the gas-tight screen: The cross sections

Fig. 13: Work space components

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50115191 A / 08.2009

of the openings are smaller when samples are
accessed.

4.10.3 Water reservoir

The water reservoir [1] is integral to the interior
container floor and inclines toward the rear. The water
level is monitored by a water level sensor [2] that
issues an alarm message at the display and an audible
signal when the water falls below the minimal level.
The embossings [3] in the water tray are used as
indicators for the maximal level.

4.10.4 Gas humidification (optional, only for O

2

control)

A hose [5] connects the gas humidification [6] to the
device-integral oxygen or nitrogen supply line [4]. The
inflowing oxygen or nitrogen is fed to the heated
water. This ensures humidification of the gases as
they enter the work space and prevents an undesired
drop of the work space humidity.

4.10.5 Heating system

An air jacket heating is used for heating the work
space. The arrangement of the heating elements
ensures that condensate formation above the water
reservoir is prevented as far as
possible.
The outer door of the device is also heated. The heat
radiated onto the interior glass door/gas-tight screen
prevents condensate formation. The work space of the
device always remains visible, despite high humidity.

Fig. 14: Water reservoir

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4.10.6 Rear panel openings

A pressure compensation opening with insert in the
rear panel of the device allows a compensation
between the pressures in the work space and in the
operating room.
A sealable access port allows cables, hoses or
additional sensors to be routed into the work space of
the device.

HERAcell

®

150i:
– access port, Ø 42 mm [1]
– pressure compensation opening [2]

HERAcell

®

240i:
– access port, Ø 42 mm [3]
– pressure compensation openingt [4]

Operating conditions:

When accessories are to be operated in the
work space of the CO

2

incubator, the
ambient condition requirements must be
observed (see table below). The energy
introduced into the work space affects the
beginning of the temperature control
range. When additional heating sources are
introduced into the work space,
condensation (e.g. at the glass door) may
occur.

Fig. 15: Rear panel openings

Introduced

energy

General

Example:
RT* = 21° C

0 W RT + 3° C 24° C

5 W RT + 6,5° C 27,5° C

10 W RT + 9,5° C 30,5° C

15 W RT + 13° C 34° C

20 W RT + 16° C 37° C

Control range of the temperature

*RT = Ambient temperature

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4.10.7 Shelf system
Fig. 16: The support rails [1] of the shelf system are

perforated every 42 mm so that the support hooks [8]
can be inserted variably for any culture container size
required. The shelves [2] have an integrated tilt
protection and withdrawal stop. For details of the shelf
system, see Section „Start-up“.

4.10.8 Bottle turning device for HERAcell® 240i

(optional)

Fig. 16: HERAcell

®

240i may be equipped with up to
four separate bottle turning devices. Each unit
consists of a roller insert [6] with a drive roller [4] and
four idle rollers [7] that are also rotated due to the
transmission function of the inserted cylinders [5].
Each unit is controlled separately as the drive roller is
connected to the device control unit by a connector [3]
at the right side of the device.
This modular layout also allows the operation of only
one bottle turning device in combination with several
shelves.
The arrangement of the idle rollers in the roller insert
frame depends on the configuration of the cylinders
used. The drive roller is always inserted into the right
roller guide. The position and the number of the idle
rollers in the frame then depend on the cylinder
diameter and on the number of cylinders. The figure
shows three examples of different idle roller positions
in dependence of the cylinder diameter.
The arrangement of the roller inserts in the work space
is designed alphabetically as a, b, c, and d with a being
the lowest and d being the uppermost roller insert.
The connections of the drive rollers are assigned
correspondingly to the device control unit. The drive
roller of the switching insert a must only be connected
to the lower receptacle a of the corresponding control
unit.

Connection assignment:

If the drive roller is not properly connected
to the control unit, the parameters cannot
be set correctly on the operating panel of
the device.

Fig. 16: Bottle turning device

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Receptacles:

To prevent corrosion due to moisture in the
work space, unassigned receptacles of the
bottle turning device must always be
plugged with the protective cap.

The rotating speed of the bottle turning devices is set
depending on two parameters:

• Diameter (of the cylinders used),

• drive roller set value.

The value is determined using a diagram (see chap.

5.4‚ pg. 51).

4.10.9 Water pump
Fig. 17: The water pump serves for suction cleaning of

the remainig water in the water reservoir. The water
drain works through gravity.

1. Place the water pump [1] on lowest shelf [5].

2. Feed the inlet hose [4] through a whole [7] of the
lowest the shelf to the water reservoir [8].

3. Prepare a bucket [2].

4. Suck the water, therefore pump the water pump
using the hand grip [6] about four times until
water flows in the outlet hose.

5. Let the water run into the bucket [2] through
gravity.

6. Wipe out the rests of the water at the floor plate of
the water reservoir.

Fig. 17: Water pump

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5 Start-up

5.1 Preparing the work space

7. Upon delivery, the CO2 incubator is not in a sterile
state. Before the initial start-up, the device must
be decontaminated.

Before the decontamination is performed, the
following work space components must be cleaned:

•Support rails,

• support hooks,

•shelves,

• bottle turning assembly (optional),

• gas humidification (optional),

• work space surfaces,

• work space seals and gaskets,

• glass door/gas-tight screen.

Decontamination:

For details about the cleaning and
disinfection of the device, (see chap. 9‚
pg. 100).

5.2 Installing the shelf system

Tools are not required for the installation of the shelf
system. The support rails are secured using spring
pressure. After the support hooks have been inserted
into the rail, the shelves are pushed onto the support
hooks.

5.2.1 Installation/removal of the support rail

The support rails are held at the sides by the
embossings [2] and [5] and secured by the
embossings [1] and [6]. The support rails marked with
the diamond shape are inserted at the rear panel of the
device with the locksprings [3] facing upward.

1. Position the support rail [4] onto the lower
embossing [6] and tilt it toward the work space
side wall so that the rail is positioned over the two
embossings [5] and [2].

2. Clamp the lockspring [3] behind the upper
embossing [1].

3. To remove the support rails, pull the lockspring
tab down out of the embossing and remove the
rail.

Fig. 18: Support rail installation/removal

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5.2.2 Installing the shelf supports:

1. Fig. 19: Insert the shelf supports [3] into the
perforations [1] of the support rail with the bar
facing down.

2. Make sure that the two vertical elements [2] of the
shelf support are flush with the support rail.

Installing the shelves:

1. Push the shelf [4] onto the shelf supports with the
tilt protection [5] facing the rear panel of the
device. The tilt protection [5] is also used as a
guide for the shelf.

2. Slightly raise the shelf so that the withdrawal stop
[6] can be routed over the shelf supports.

3. Make sure that the shelf support is positioned in
the two tilt protections in a way that allows it to
move freely.

5.2.3 Levelling the device

1. Position a bubble level onto the center shelf or
onto the roller holder.

2. Rotate the adjustable device stands using the
supplied 24 mm wrench until the shelf is
positioned horizontally in all directions. Perform
the adjustment of the device stands from left to
right and from rear to front.

5.3 Installing the gas humidification
(optional, only for O2/N2-control)

Fig. 20: The gas humidification [1] is installed to the

water tray parallel to the rear panel of the device. The
position to the right side wall is predetermined by the
hose length.

1. Install hose [2] to the sleeve of the gas
humidification and then to the sleeve [3] of the
device-integral oxygen or nitrogen supply line.

2. Place gas humidification immediately to the rear
panel of the device and align it parallel to the rear
panel.

Fig. 19: Shelf support installation

Fig. 20: Installing gas humidification

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5.4 Installing the optional bottle turning
devices HERAcell® 240i

1. Align the roller insert so that the roller holder [8]
and the crossmember [7] are flush and facing the
work space opening.

2. Slide the roller insert with both guide rails [5] onto
the shelf supports.

3. Slightly raise the roller insert so that the
withdrawal stop can be routed over the shelf
support.

4. Insert the drive roller [6] into the right receptacle
and connect the cable [4] to the corresponding
control unit a.

5. Insert the four idle rollers [3] the into receptacles
and position the rollers in the receptacles in
accordance with the bottle diameter.

6. Place the bottles onto the rollers. To ensure that
the bottles will not be shifted against each other
by the rotation, the bottoms of the bottles should
contact the stop disks [2] of the rollers and the
cylinder necks [1] should contact the rubber stop
rings [8]. The stop ring can be positioned
accordingly on the roller.

Set speed of the bottle turning device:

7. The rotating speed of the bottle turning devices is
set depending on two parameters:
– Diameter (of the bottles used),
– drive roller set value.

Use of diagram:

8. The diagram shows the common bottle diameters
as different graphs (ascending straight).
– Set the desired speed at the Y axis. Read the

corresponding set value (in %) from the X axis
at the point of intersection of the Y value with
the specific graph.

Fig. 21: Installing the bottle turning devices

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Example:

– Bottle diameter: 56 mm
– Desired speed: 3.5 rpm
– Determined set value: 48%

In the dialog box S

PEED BOTTLE TURN. DEVICE, the value 48% is set (see chap.

7.11.3‚ pg. 85).

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Fig. 22: Bottle turning speed

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5.5 Installing the optional center strut
HERAcell® 240i for split inserts

If HERAcell® 240i is equipped with the optional 6­segment insert, two center struts [1] with perforations
at each side must be installed.
In this case the shelf support rails must be installed to
the support rails at the sides and at the left and right
side of the strut.
The support rails marked with the diamond shape are
installed at the rear panel of the device.

1. A pressure spring keeps the upper rectangular
guide [3] of the center strut under tension. Insert
this guide into the receptacle [2] in the work space
ceiling und push it slightly upward so that it is
pushed into the center strut.

2. Then, position the lower rod guide [5] of the
center strut into the hole [6] in the work space
floor and remove spring tension.

3. The center strut is stabilized by the spring
pressure.

Configuration with split inserts:

For versions with split inserts, bottle
turning devices cannot be installed.

Fig. 23: Center strut installation

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5.6 Gas connection

Gas quality:

The gases must have one of the following
qualities:

• Purity 99.5 % min,

• medical gas quality.

Overpressure!

The operating pressure of the gas applied
to the device must not exceed 1 bar. If the
gas is supplied at a higher pressure, the
valves integral to the device may not close
correctly and the gas supply control may
be impaired.
Set the gas supply to a range between 0.8
bar min. and 1.0 bar max. and make sure
that this pressure setting cannot be
changed!

5.6.1 Installing gas pressure hoses

The gas supply from the gas supply system to the
device is achieved using the supplied flexible gas
pressure hoses:

1. Connect the gas pressure hose [2] to the sleeve of
the gas supply system.

2. Remove the protective cap [3] from the sterile
filter.

3. Slide the hose clamp [1] onto the gas pressure
hose and connect hose to the sleeve [4] of the
sterile
filter [5].

4. Secure the gas pressure hose to the sleeve of the
sterile filter using the hose clamp.

Pressure compensation opening:

To ensure permanent pressure
compensation, the pressure compensation
opening must not be connected to an
exhaust air system. The pipe of the

Fig. 24: Installing gas pressure hoses

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50115191 A / 08.2009

pressure compensation opening must not
be extended or redirected.

5.6.2 Installing devices without the optional gas
monitoring system

The gas supply from the gas supply system to the
device is established using the supplied flexible gas
pressure hoses (see chap. 5.6.1‚ pg. 55):

CO

2

connection:

• For a device with CO

2

connection, connect the gas
supply to the sterile filter [2] in accordance with the
connection diagram [1].

Combined CO

2

and O2/N2 connection (optional):

For a combined CO

2/O2/N2

connection, proceed as
follows in accordance with the connection diagram
[3]:

• Connect the O

2/N2

supply line to the upper gas

inlet filter [4],

• connect the CO

2

supply line to the lower gas inlet

filter [5].

Manual gas monitoring:

On devices without the optional gas
monitoring system,
there is no automatic gas monitoring.
Therefore, the filling level of the gas bottles
must be checked every day.

Fig. 25: Gas connection without gas monitoring system

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5.6.3 CO2 connection with gas monitoring

system (optional)

The gas supply from the gas supply system to the
device is established by the supplied flexible gas
pressure hoses.
Fig. 26: Devices with CO

2

connections that are
equipped with the optional gas monitoring system are
connected according to the connection diagram [7].

CO

2

connection:

For a device that is operated with process gas CO

2

and equipped with the optional gas monitoring
system, connect the gas supply lines as follows:

• Distributor connection [5] for the CO

2

gas supply
for up to three additional devices. For looping the
gas supply through, interconnect the devices with
a gas pressure hose.

• Connect the gas container B to the upper
connection [2] of the gas monitoring system,

• connect the gas container A to the lower
connection [3] of the gas monitoring system.

• The outlet of the gas monitoring system [4] is
connected at the factory to the gas inlet filter [5] by
a short gas pressure hose [6].

Looping the gas supply through:

By looping the gas supply through, up to 4
devices can be supplied by a CO

2

gas

supply.

5.6.4 Combined CO

2/O2/N2

connection with gas

monitoring system (optional)

The gas supply from the gas supply system to the
device is established by the supplied flexible gas
pressure hoses (see chap. 5.6.1‚ pg. 47).
Fig. 27: Devices with the combined CO

2/O2/N2

connection that are equipped with the optional gas
monitoring system are connected according to
connection diagram [13] and have the following gas
connections:

O

2/N2

supply:

• Distributor connection [1] for the O

2/N2

gas supply
for up to three additional devices. For looping the
gas supply through, interconnect the devices with
a gas pressure hose.

• Connect the gas container B [2] to the upper

Fig. 26: Gas connection with gas monitoring system (optional)

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50115191 A / 08.2009

connection of the gas monitoring system,

• connect the gas container A [3] to the lower
connection of the gas monitoring system,

• The outlet of the gas monitoring system [4] is
connected at the factory to the gas inlet filter [9] by
a short gas pressure hose [11].

CO

2

supply:

• Distributor connection [5] for the CO

2

gas supply
for up to three additional devices. For looping the
gas supply through, interconnect the devices with
a gas pressure hose.

• Connect the gas container B to the upper
connection [6] of the gas monitoring system,

• connect the gas container A to the lower
connection [7] of the gas monitoring system.

• The outlet of the gas monitoring system [8] is
connected at the factory to the gas inlet filter [10]
by a short gas pressure hose [12].

Looping the gas supply through:

By looping the gas supply through, up to 4
devices (see annex at the end of this
section) cyn be supplied by a CO

2

gas

supply or O

2/N2

gas supply.

Fig. 27: Combined connection with

gas monitoring system (optional)

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5.7 Power supply connection

Electric shock!

Contact with current-carrying components
may cause a lethal electric shock.
Before connecting the device to the power
supply, check plug and power supply cable
for damage.
Do not use damaged components when
connecting the device to the power supply!

The device must be connected only to a correctly
installed and grounded power supply source:

•Fusing T 10 A

• Circuit breaker G 16

Connection to the power supply system:

1. Before connecting the device to the power
supply, check to see if the voltage of the power
supply corresponds with the specifications on the
nameplate on the front of the device. If the ratings
given for voltage (V) and current (A) are not
correct, the device must not be connected to the
power supply.

2. Connect the IEC connector [2] to the socket [1] of
the control box of the device.

3. Connect the grounding plug [3] of the power
supply cable to a correctly grounded and fused
socket.

4. Make sure the power supply cable is not
subjected to tensile or compressive force.

Fig. 28: Combined gas connection with gas monitoring system

Fig. 28: Power supply connection

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5.8 Connecting the RS 232 interface

Data communication via RS 232 The RS 232 data communication interface has been designed for a cable

connection with 9-pin connector and a contact assignment of 1:1.
Data exchange is accomplished by a predefined structure of command
sequences (see annex at the end of this section).

Data communication diagram:

The command sequence syntax as well as the RS 232 interface
data communication diagram are described in the annex at the
end of this section.

Connecting the devices:

1. Turn the PC off.

2. Connect the serial interface cable connector (not included in the
standard equipment) to the socket at the device supply interface at the
rear of the device.

3. Connect the second connector to an unassigned serial port COM 1 /
COM 2 etc. at the PC.

4. Turn the PC on.

5.9 Connecting the USB interface (optional)

Data communication via USB As an alternative to data communication via RS 232 interface, the devices

can be equipped with the optional USB interface. The USB interface
complies with Standard USB 1.1 and is compatible with Standard USB 2.0
(full speed).
The interface stepping rate can be changed within the defined baud rates
(9,600, 19,200, 38,400, 57,600 baud).
Data exchange is accomplished via a defined command sequence
structure (frames). The command sequences correspond with the RS 232
interface installation diagram (see annex at the end of this section).
Using the USB interface for data communication between a PC and the
device a driver must be installed first (see annex data communication).

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5.10 Connecting the alarm contact

Skilled work:

Thermo Scientific warrants the operational safety and the
operativeness of the device only if installation and repairs are
performed properly.
The connection of the device to an external alarm system must
only be carried out by adequately trained and authorized
expert electrical/telecommunication personnel!

Function:

When system errors and failures occur in the temperature or gas control
circuits, an alarm message is issued to the connected alarm/monitoring
system. The potential-free contact (1 changeover contact) has been
designed for the following circuits:

Alarm relay:

Switching structure:

The alarm relay switches for all failures reported by the control
loops (see chap. 7.13‚ pg. 95).

Circuit Voltage External fusing

Circuits with sy ste m voltage max. 250 V ~ max. 6 A

25 V ~ max. 2 A

60 V = max. 1 A

50 V ~ max. 1 A

120 V = max. 0.5 A

SELV circuits (cf.
VDE 0100, Part 410)

SELV-E circuits (cf. VDE 0100,
Part 410)

Operating states

Contact

4 - 1

Contact

4 - 3

Operating state power failure "off" X O

Operating state power failure "on" O X
Failure: power failure "off"

OX

Failure: power failure "on"

XO

X: Contact closed / O: Contact open

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Connection example:

The connector [5] for the connecting cable is included
in the standard equipment. The values for the
operating voltage of the external circuits and of the
fusing of the alarm system are given in the table on the
previous page.

1. Connect the individual conductors [1] to [4] of the
connecting cable as shown in the wiring diagram.

2. Connect the connector [5] of the alarm system
connecting cable to the interface [6] at the control
box at the rear panel of the device.

Fig. 29: Connection example

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6 Operation

6.1 Preparing the device

The device must only be released for operation after all major measures for
the start-up have been taken (see Section 5, page 45).

Device check:

Device check Prior to starting operation, the following device components must be

checked for their correct function

• Gas hoses must be seated tight on the connecting filter and must be
secured using a hose clamp.

• The access port must be capped.

• The pressure compensation opening must be permeable, its insert
must be installed in the work space.

• The glass door/gas-tight screen seal must not be damaged.

• The glass door measurement opening must be capped.

• The shelf system components must be installed safely.

• The gas humidification (optional) must be connected to the device­integral gas supply and aligned parallel to the rear panel.

• The drive rollers of the bottle turning device (optional) must be properly
connected to the corresponding control unit.

• The idle rollers of the bottle turning device (optional) must be
positioned in accordance with the cylinder diameters.

Decontaminating the device work space:

Decontamination Run the contra-con decontamination routine (see chap. 9.4‚ pg. 104) or

decontaminate the work space according to the hygiene regulations set
forth by the operator.

Hygiene regulations:

Prior to any operation, the user must clean and disinfect the
work space in accordance with the hygiene regulations set
forth by the operator to protect the cultures.
The “Principles of good microbiological proceedings” at the
annex of these instructions can be used as safety information
for personnel working with the device.

Water supply:

A sufficient quantity of treated water of the following quality
must always be available for the running operation:

• distilled, fully demineralized, and

• autoclaved, sterile.
When the filling level drops below a minimum value, it must be
possible to top up water during the work process.
Capacities:

•HERAcell

®

150i: 3.0 l

•HERAcell

®

240i: 4.5 l

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6.2 Starting operation

1. Fill the water tray with a sufficient quantity of processed water. Do not
exceed the upper level mark.

2. Make sure that the CO

2/O2/N2

supply system valves are open.

3. Turn the device on using the power switch.

4. Set the set values for temperature and CO

2

/ O2 content on the iCan™

touchscreen.

5. Start contra-con.

Starting the device:

Starting with auto-start 6. Start the device with the auto-start routine (see chap. 7.9.1‚ pg. 75).

7. The progress indicator auto-start appears on the display, and the
automatic start routine is run.

8. The temperature control adjusts the temperature to the selected set
value, humidity rises.

9. When temperature and relative humidity are constant, the automatic
adjustment of the CO

2

/ O2 measuring system is performed

10. The CO

2

/ O2 control supplies the set amount of CO2 / O2.

11. If the auto-start routine has been completed, the progress indicator is
hidden, and the main menu is displayed. The device is operational.

Loading the device:

Loading the work space 12. Load the work space with cultures or place bottles onto the bottle

turning device rollers.

Duration of the auto-start routine:

When the device is cold and when the ambient temperature is
low, the auto-start routine may take up to 10 hours.

Loading:

To ensure sufficient air circulation and even heating of the
samples, the loading surface within the work space should be
used up to 70% max. Voluminous objects in the work space
that dissipate heat may impair heat distribution.

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7 Handling and control (iCan™ touchscreen controller)

7.1 Power switch

Fig. 30: Depending on which side the door hinges are

installed, the power switch [1] is integral to the front
cover [2] of one of the front device stands.

• To turn the device on: Press the power switch [1];
the switch illumination comes on.
– After a short audible signal and a short dark

phase, the touchscreen display appears.

– The control loop sensors pass the heat-up

phase (see chap. 7.4‚ pg. 68)

• To turn the device off: Press the power switch; the
switch illumination goes off

7.2 Operating panel and operating
structure

Fig. 31: The control panel works as a touchscreen

(iCan™ touchscreen) and can be controlled
performing light pressure on the pressure-sensitive
areas of the screen using a finger or a blunt pin:

• Temperature display [1],

•CO

2

display [3],

•O

2

display [5] (optional).

•contra-con key [2]

• menu key [4]

•auto-start key [6],

Extended pressure-sensitive area:

To accept a failure message, the entire
touchscreen can be used as a pressure­sensitive area.

.

Fig. 30: Power switch

Fig. 31: Pressure-sensitive areas

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Versions without O2/N2 control:
Fig. 32: Function keys and value displays of the

operating panel for a device version without O

2/N2

gas

supply:

[1] Type designation of the device
[2] Temperature display
[3] CO

2

display

[4] Key for starting the contra-con

decontamination routine
[5] Key for opening the menu navigation
[6] Key for starting the auto-start routine
[7] Actual value CO

2

[8] Set value CO

2

[9] Temperature actual value

[10] Temperature set value
[11] Current date and time

Version with combined CO

2/O2/N2

control

(optional):
Fig. 33: Function keys and value displays of the

operating panel of a device version with combined
CO

2/O2/N2

gas supply:
[1] Type designation of the device
[2] Temperature display
[3] CO

2

display

[4] CO

2

set value

[5] CO

2

actual value

[6] Key for starting the contra-con

decontamination routine
[7] Key for opening the menu navigation
[8] Key for starting the auto-start routine
[9] O

2

display

[10] O

2

actual value

[11] O

2

set value
[12] Temperature actual value
[13] Temperature set value
[14] Current date and time

Structure of the operating levels:
Fig. 34: Operation is divided into three levels:

• A: Direct access to the control loop settings:
Temperature, CO

2

, O2 set value and bottle turing

device (optional),

• B: Start of the device routines contra-con or auto­start,

• C: Navigation through the submenus for device
configuration.

Fig. 32: iCan™ touchscreen withoutO2/N2gas supply

Fig. 33: iCan™ touchscreen with combined gas connection

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Fig. 34: Menu structure overview

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50115191 A / 08.2009

7.3 Factory presettings of the iCan™
touchscreen controls

Upon delivery of the device, the following set values
have been preset:

• Temperature: 37 °C

•CO

2

content: 0.0%

•O

2

content (optional): 21.0%

• Optional bottle turningdevice speed a, b, c, and
d: 0%.

CO

2/O2

control:

Since the CO

2

concentration of the air is

nearly 0%, the CO

2

control and the control
loop error monitoring system are disabled
at a set value of 0%.
Since the O

2

concentration of the air is

nearly 21%, the O

2

control and the control
loop error monitoring system are disabled
at a set value of 21%.

7.4 Heat-up phase of the control loop
sensors

When the device has been switched on, the control
loop sensors pass through a heat-up phase of varying
duration during the start process:

Temperature control loop 10 s
CO

2

control loop 10 s

CO

2

control loop with IR measuring cell 3 min

O

2

control loop 5 min

The start process is indicated by an audible signal.
During the heat-up phase, the displays show dots (...)
instead of values:

• Temperature display [2],

•CO

2

display [1] and

•O

2

display [3]
After the heat-up phase has been completed, the
control loop actual values are indicated.

Fig. 35: Heat-up phase display

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Exposure to CO2 gas:

During the 5-minute heat-up phase of the O

2

control loop, the

work space is not exposed to CO

2

gas and the CO2 control and

is disabled.

7.5 Event actions for settings

Key response:

Pressing a key can increase or reduce a value gradually:

• When the – key [4] or the + key [6] is kept depressed, the system
switches to quick run,

• after the key has been kept depressed for more than 3 seconds, the
quick run speed increases.

Saving the settings:

To save changed values, press the Enter key.

Resetting the settings:

Unless a user action (contact with the pressure-sensitive areas
and keys) occurs within 30 seconds, the system automatically
exits the menu and the most recently confirmed setting
defaults.

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7.6 Setting the temperature set value

1. Press the TEMPERATURE DISPLAY key [1].

> The Temperature menu is displayed.

2. To exit the Temperature menu:

> Press the E

ND key [2].

3. To set the temperature set value:

> Press the S

ET VALUE key [3].

To increase the set value:
> Press the + key [6].

To reduce the set value:
> Press the - key [4].

4. To accept and save the set value:

> Press the E

NTER key [5].

> The system returns to the main menu. The

temperature displays shows the actual value
currently measured in the work space.

Fig. 36: Setting the temperature set value

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7.7 Setting the CO2 set value

1. Press the CO2 DISPLAY key [1].
> The CO

2

menu is displayed.

2. To exit the CO

2

menu:

> Press the E

ND key [2].

3. To set the CO

2

set value:

> Press the S

ET VALUE key [3].

To increase the set value:
> Press the + key [6].

To reduce the set value:
> Press the - key [4].

4. To accept and save the set value:

> Press the E

NTER key [5].

> The system returns to the main menu. The CO

2

display shows the actual value currently
measured in the work space.

Deactivating the CO

2

control loop:

To deactivate the CO

2

control, the set value
is set to 0%.
If the control loop is deactivated, error
monitoring is enabled as well.

Gas monitoring:

The switching state of the CO

2

control loop
does not affect the function of the optional
gas monitoring system. The gas monitoring
system remains active even when the CO

2

control is switched off.
For set values of 21% O

2

, the O2 control is
switched off and error monitoring is
disabled as well.
The control loop error monitoring system
(optional) is enabled.

Fig. 37: Setting the CO

2

set value

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7.8 Setting the O2 set value

This setting is possible only on versions with the
optional O

2/N2

control.

1. Press the O

2

DISPLAY key [1].

> The O

2

menu is displayed.

2. To exit the O

2

menu:

> Press the E

ND key [2].

3. To set the O

2

set value:

> Press the S

ET VALUE key [3].

To increase the set value:
> Press the + key [6].

To reduce the set value:
> Press the - key [4].

4. To accept and save the set value:

> Press the E

NTER key [5].

> The system returns to the main menu. The O

2

displays shows the actual value currently
measured in the work space.

Factory settings:

Depending on the type of the O

2

sensor,

one of the two O

2

control ranges has been
preset at the factory:
Control range I: 1% to 21%
Control range II: 5% to 90%

Usage of the process gases:

For set values below 21% O

2

, the device
must be connected to a nitrogen supply
system.
For set values above 21% O

2

, the device
must be connected to an oxygen supply
system.
Since the O2 concentration of the air is
nearly 21%, the O2 control and the control
loop error monitoring system are disabled
at a set value of 21%.
The control loop error monitoring system
(optional) is enabled.

Fig. 38: Setting the O

2

set value

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7.9 Auto-start routine

The auto-start function is an automated routine for the start and the
subsequent adjustment of the CO

2

measuring system. After the start, the
device control adjusts the temperature to the set value while humidity is
generated. When temperature and relative humidity have reached constant
values, the CO

2

measuring system is automatically adjusted to these

values, and the work space is supplied with the preset quantity of CO

2

.

Application of the auto-start routine:

To ensure that the specified accuracy of the CO

2

measuring
system is maintained, the device should always be started
using the auto-start routine if

• a difference of more than 1 °C is entered upon setting the

temperature set value,

• the Low Humidity function is enabled/disabled,

• the device is restarted after an extended interruption of

operation.
The auto-start routine should be run at least every three
months on the occasion of cleaning and maintenance works.

Duration of the routine:

Running the routine usually takes 5 to 7 hours. At low room
temperatures and when the device is cold, it may take up to 10
hours until the auto-start routine has been completed. If the
glass door is opened or if the power supply of the device is
interrupted while the routine is running, the routine is
interrupted and rerun after the glass door has been closed or
the power supply has been reestablished.

Start conditions for the auto-start routine:

Prior to running the auto-start routine, set the CO

2

and O2 set
values to the desired values and make sure that the
atmosphere in the work space only consists of ambient air.
The floorpan of the device must be filled with a sufficient
quantity of water.

Conditions that prevent the start of the auto-start routine:

The auto-start routine cannot be started if one of the following
failure conditions exists.
Temperature control loop:

• Sensor breakage,

• actual value above set value (excessive deviation),

• actual value below set value (excessive deviation),

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• set value not plausible,

• calibration values too high or too low,

• sensor communication failure,

• sensor parameter not plausible,

• no communication with LM 75.

Control loop for CO

2

gas supply:

• No communication with sensor.
In this case, the auto-start key is dimmed and its function is not
available.

Faulty cancellation of the auto-start routine:

The auto-start is cancelled if:

• an error is detected in the temperature control loop,

• an error is detected in the CO

2

control loop,

• the water filling level is insufficient.

auto-start-Dry execution of auto-start routine:

If the auto-start routine is to be run dry (without water in the
floorpan of the work space), the water level sensor must be
disabled prior to its start (see chap. 7.11.3‚ pg. 85).

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7.9.1 Activating auto-start
Preparations for the start:

1. Make sure that the CO

2

-/O2-/N2 gas supply system

valves are open.

2. Fill the floorpan of the work space with a sufficient
quantity of water. The filling level must not
exceed the upper filling level mark.

3. Set the set values for temperature, CO

2

and O2 on

the touchscreen.

auto-start routine activation:

1. Press the auto-start key [2].
> The auto-start-instruction menu [1] is

displayed.

2. To exit the auto-start-instruction menu and
cancel auto-start:

> Press the E

ND key [4].

3. Activate the auto-start routine:

> Press the S

TART key [3].

4. To air the work space, open both device doors.

5. When the audible alarm sounds after 30 seconds,
close both device doors.
> The trend status indicator [5] is displayed.

6. During the progress, the status indicator displays
the following information:
– Temperature,
– start time.

Cancellation:

The auto-start routine can be cancelled at
any time!
Press the Stop key [6].

Automatic restart:

The auto-start routine is restarted
automatically if the routine is cancelled due
to one of the following events:

• The glass door is opened,

Fig. 39: Activating the auto-start routine

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• the outer door is opened on a device
with optional gas-tight screen,

• the power supply is interrupted.

7.9.2 Interrupting the auto-start routine
If the S

TOP key in the status display is depressed (see

above), the auto-start routine is interrupted and the
auto-start stop dialog box is displayed for a safety
scan. The routine can now be permanently cancelled
or resumed.

1. To resume the auto-start routine:

> Press the B

ACK key [1].

> The system returns to the status display, and

the auto-start routine is resumed.

2. To cancel the auto-start routine:

> Press the E

ND key [2].

> The warning triangle [3] is displayed as a failure

message together with the audible signal.

3. To accept the failure message:

> Press any position on the display.
> The E

RROR dialog box with the description of

the error is displayed.

4. To end the auto-start routine:

> Press the E

ND key [4].

> The system returns to the main menu.

7.10 Running the contra-con routine

contra-con is an automatic decontamination routine
for disinfecting the device work space. For a detailed
description of this device function, (see chap. 9.4‚
pg. 104).

7.11 User configuration

The user configuration settings allow the user
interface and the additional device functions to be
adjusted to the requirements of everyday operation.
The user configuration menu is split into six
categories:

• Settings,

•Event logging,

•Options,

• Icon description,

Fig. 40: Interrupting the auto-start routine

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•Keypad lock,

• Software versions.
To make a user-specific setting in a dialog box,
navigate through the submenus listed in the
illustrations and open the dialog box.

7.11.1 Settings
The input dialogs of the S

ETTINGS category comprise

all settings for the individual configuration of the user
display screen and the user interface:

• Keypad lock coding,

• Date/time setting,

• Contrast setting,

• Key tone setting,

• Interface configuration,

• User display screen language setting,

• Reminder interval setting.

Changing the keypad lock code:

The keypad lock prevents the unauthorized changing
of the operational settings. Only those keys are locked
where values can be entered.
The keypad is locked by entering four numbers.

• Upon delivery, the default code is: 0000.
This default can be changed into a user-defined code
that is then enabled using the

KEYPAD LOCK dialog box

(see chap. 7.11.5‚ pg. 92).

1. To enter the default 0000:

> Press the corresponding numeric keys.
> The number combination is displayed hidden in

the input box [1].

2. To delete the input numbers:

> Press the D

ELETE key [2] .

3. To exit the menu:

> Press the B

ACK key [4] .

4. To confirm the input:

> Press the E

NTER key [3].

> The system changes to the C

ODE NEW menu.

Entering the new 4-digit code:

> Press the corresponding numeric keys.
> The number combination is displayed in the

input box [1].

Fig. 41: Changing the code

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Fig. 41: To set the cursor to the left to overwrite a
value:

5. Press the B

ACKSPACE key (<<) [6].

6. To accept and save the input value:

> Press the E

NTER key [5].

> The system returns to the Settings menu.

Changing the user-defined code:

The user-defined code can be changed as
often as required using the same
procedure:

• Activate the recoding function by
entering the valid code,

• enter the new code and confirm it.

Setting date / time:

The input dialog allows date and time to be set to the
required time zone.

1. Fig. 42: To enter the date:

> Press the numeric key [2].
> The input numbers are displayed in the input

box [1].

2. To set the cursor to the left to overwrite a value:

> Press the B

ACKSPACE key (<<) [4].

3. To accept and save the input value:

> Press the E

NTER key [3].

4. The system returns to the Date/Time menu.

5. Enter the time accordingly.

6. To accept and save the input value:

> Press the E

NTER key [3].

> The system returns to the Date/Time menu.

Fig. 42: Date / time setting

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Setting the contrast:

The input dialog allows the color contrast of the
operating panel to be set within the value range of
48% to 80%.

1. To increase the value:
> Press the + key [4].

2. To reduce the value:
> Press the - key [2].

3. The value change appears in the display [1].

4. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Settings/Setup

menu.

Setting the key tone:

The input dialog allows the loudness of the key tone
that sounds whenever a key is depressed to be set.
The value range is 0 to 100. The change occurs in
increments of 5%.

1. To increase the value:
> Press the + key [4].

2. To reduce the value:
> Press the - key [2].

3. The value change appears in the display [1].

4. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Settings/Setup

menu.

Fig. 43: Display contrast setting

Fig. 44: Key tone setting

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Setting the interface baud rate:

The input dialog allows the setting of the stepping rate
for data communication of the:

• RS232 interface,

• USB interface (optional).

The stepping rate of both interfaces can be changed
within the defined baud rates (9.600, 19.200, 38.400,

57.600 baud).
The standard stepping rate of both interfaces is 57.600
baud.

1. To increase the value:
> Press the + key [4].

2. To reduce the value:
> Press the - key [2].

3. The value change appears in the display [1].

4. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Interface menu.

5. To activate the new settings:

> Browse back to the main menu.
> Wait for approx. 10 seconds and perform a

reset by switching the device off and on using
the power switch.

Fig. 45: RS 232 interface baud rate setting

Fig. 46: USB interface baud rate setting

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Setting the user display screen language:

The input dialog allows the language of the display
screen to be set. Five languages are available:

•German,

• English,

•Spanish,

• French,

•Italian.

1. To browse upward in the selection:
> Press the + key [4].

2. To browse downward in the selection:
> Press the - key [2].

3. The new language appears in the display [1].

4. To accept and save the selection:

> Press the E

NTER key [3].

> The system returns to the Settings menu.

Fig. 47: Language setting

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Setting the reminder intervals:

The reminder intervals are integral components of the
alarm and monitoring system of the device control.
For the two essential functions contra-con and auto­start as well as for routine service work, the user can
set dates that trigger an alarm whenever they occur.
The counting begins at 00:00 hrs of the day on which
the previously set reminder interval has elapsed.

1. To increase the number of days:
> Press the + key [4].

2. To reduce the number of days:
> Press the - key [2].

3. The value change appears in the display [1].

4. To deactivate the reminder interval:

> Set the value to O

FF.

> Press the - key [2].

5. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Reminder Interval

menu.

On the due date, the display shows a reminder
message [5] for the activated reminder interval.

• contra-con: Please run contra-con.

• auto-start: Please run auto-start. Is displayed after
the contra-con decontamination routine has been
run successfully.

• Service interval: Request service. The service
message can be confirmed. In this case, the
Request Service icon is displayed.

After the routines have been run successfully, the
reminder messages are hidden.

Factory settings:

contra-con decontamination routine 90 days
auto-start routine Off
Service interval 365 days

Fig. 48: Reminder interval setting

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7.11.2 Event logging

The input dialogs of the category

EVENT LOGGING

comprise all settings for logging and outputting events
during the operation of the device:

• Event display,

• time interval (of the logging cycle),

•error table.

Event display:

The event display [1] uses short, single-line entries
specifying date and time to report the events that were
logged during the operation of the device. The entries
are listed in chronological order with the most recent
event at the top position. The list can be displayed but
not edited. If the event display consists of several
pages, the user can browse through the list. The status
indicator [2] indicates which page of the total number
of pages is currently being displayed.

1. To browse forward in the list:

> Press the C

ONTINUE key [5].

2. To browse backward in the list:

> Press the P

REVIOUS key [3].

3. To exit the display:

> Press the E

ND key [4].

> The system returns to the Event Logger menu.

Changing the logging cycle time:

Due to limited memory resources, the oldest entries
are deleted as new entries are logged. The period from
which the displayed entries originate depends largely
on the selected logging cycle time.

The setting controls the logging cycle in sections of
seconds during which the control loop measured
values are logged during the operation of the device
and displayed by the trend display (see chap. 7.12‚
pg. 94).

Fig. 49: Event display

Fig. 50: Logging cycle setting

Logging cy cle time Minimal displaye d pe riod

10 s 22.5 hours

30 s 2.8 days

60 s 5.6 days

120 s 11.2 days

180 s 16.8 days

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Fig. 50: The settings can be made within the value
range of 10 seconds to 3600 seconds.

1. To increase the value:
> Press the + key [4].

2. To reduce the value:
> Press the - key [2].

3. The value change appears in the display [1].

4. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Event Logger menu.

Event logger interval time:

The logging cycle time does not affect the
entries of the error table.

Displaying the error table:

The error table lists the errors detected by the device­integral monitoring system in descending
chronological order. The most recently detected error
is listed at the top position of 22 possible entries. An
entry consists of the control loop in which the error
occurred, the date, the time, and an error description.
The error table can be displayed but not edited.
If the event display consists of two pages, the user can
browse through the list. The status indicator [2]
indicates which page of the two is currently being
displayed.

1. To browse forward in the error table:

> Press the C

ONTINUE key [4].

2. To browse backward in the list:

> Press the P

REVIOUS key.

3. To exit the display:

> Press the E

ND key [3].

> The system returns to the Event Logger menu.

Fig. 51: Error table display

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Troubleshooting:

For a detailed overview of causes for errors
and their correction, please refer to the end
of this chapter!

7.11.3 Options
The input dialogs of the O

PTIONS category comprise all

settings of the functional device options:

• Alarm relay,

• Low humidity,

• Gas-tight screen,

• Water level sensor,

•Audible alarm,

• Bottle turning device (optional),

•O

2

(optional).

Setting the alarm relay:

The alarm relay is the interface for the connection of
the device-integral monitoring system to an external
monitoring system. Depending on the required input
signal of the external monitoring system, network
monitoring can be enabled or disabled. If network
monitoring is enabled, a power failure is detected as
an error. The relay cannot be switched off (see chap.

5.10‚ pg. 61).

1. To toggle between two states [1]:
> Press the + key [4].
or
> Press the - key [2].

2. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Options menu.

Fig. 52: Alarm relay setting

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Setting low humidity:

If condensation occurs on the culture containers due
to high relative humidity, the humidity in the work
space can be set to a lower level. At the factory, the
device control is preset to high humidity (approx. 93%
relative humidity).
By enabling the Low Humidity feature, the relative
humidity in the work space is lowered from approx.
93% to approx. 90%. The modification requires an
extended adaption phase. To effectively prevent
condensation on culture containers, it must be used as
a permanent setting.

1. To toggle between two states [1]:
> Press the + key [4].
or
> Press the - key [2].

2. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Options menu.

Low humidity:

The enabling/disabling of the Low
humidity function is entered into the event
list.
If the Low humidity function is enabled, the
corresponding icon [5] is displayed in the
main menu.

Fig. 53: Low humidity setting

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Setting the gas-tight screen:

As the aperture cross-sections are smaller when
accessing the samples, devices equipped with the
optional gas-tight screen achieve shorter recovery
times for the incubation parameters:

• Work space temperature,

•CO

2

concentration,

•O

2

concentration,

• relative humidity.

After conversion of the device, the device control
system must be adjusted to the gas-tight screen
option.

Malfunction:

The conversion to gas-tight screen causes a
change of the control parameters.
Failure to set the gas-tight screen function
in accordance with the actually installed
door version may impair the incubation
performance.

1. To toggle between two options [1]:
> Press the + key [4].
or
> Press the - key [2].

2. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Options menu.

Switching the water level sensor on/off:

For incubation operation with ambient humidity or if
the auto-start routine is to be run dry (without water),
the water level sensor can be switched off.
This prevents alarm messages for the water level
sensor by the device-integral monitoring system from
being issued.

1. To toggle between two states [1]:
> Press the + key [4].
or
> Press the - key [2].

2. To accept and save the change:

Fig. 54: Gas tight screen setting

Fig. 55: Water level setting

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> Press the ENTER key [3].
> The system returns to the Options menu.

Switching the audible alarm on/off:

If the device-integral monitoring system detects an
error:

• an audible alarm sounds

• in addition to the visual error message and the
switching of the alarm relay.

The audible alarm can be permanently disabled:

1. To toggle between two states [1]:
> Press the + key [4].
or
> Press the - key [2].

2. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Options menu.

Setting the bottle turning device speed (option
available only on HERAcell

®

240 i):

If the device has been configured at the factory for the
bottle turning device function, the main menu displays
the bottle turning device icon.
The speeds of the drive rollers can be set separately
for each level within a value range of 0% to 100% (see
chap. 4.10.8‚ pg. 47).
The drive roller of a bottle turning insert is activated as
soon as a value> 0 for the speed has been entered and
saved.

1. To increase the value:
> Press the + key [4].

2. To reduce the value:
> Press the - key [2].

3. To switch the drive roller of one level off:
> Set the value to 0%.

4. The value change appears in the display [1].

5. To accept and save the change:

> Press the E

NTER key [3].

> The system returns to the Bottle Turning Device

menu.

Fig. 56: Alarm relay setting

Fig. 57: Bottle turning device speed setting

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Quick access to bottle turning device
levels:

To directly access the Bottle turning device
submenu, touch the Bottle turning device
icon in the main menu.

Switching the O

2

control on and off:

Depending on the requirements to the work process,
the O

2

control can be switched on and off. This setting

is possible only on versions with the optional O

2/N2

control.

1. To toggle between two states of the O

2

control:
> Press the + key [4].
or
> Press the - key [2].

2. The value change appears in the display [1].

3. To accept and save the setting:

> Press the E

NTER key [3].

> The system returns to the Options menu.

O

2

value display:

If the O

2

control has been switched off, the

O

2

display does not show an actual value (-

- -).
This procedure provides protection for the
O

2

sensor.

If the set value is set to 21%, the O

2

control
loop is not monitored. This applies to both
O

2

control ranges:

• Control range I: 1% to 21%

• Control range II: 5% to 90%
In this case, the O

2

display shows the

actual value.

Airing the work space:

If the device was operated with O

2

or N2 ,

the work space must be aired after the O

2

control has been switched off.

Fig. 58: Switching the O2 control on and of

f

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Gas monitoring:

The switching state of the O

2

control loop
does not affect the function of the optional
gas monitoring system. The gas monitoring
system remains active even when the O

2

control is switched off or the O

2

sensor is

disabled.

7.11.4 Icon description

Essential operating states or error messages, e.g.
keypad lock [3], low humidity [4] or bottle turning
device [5] are displayed as icons in the touchscreen
main menu in addition to the entries in the event or in
the error table.
The Icon Description dialog box [1] explains the
meaning of the individual icons.

• To exit the display:

> Press the E

ND key [2].

> The system returns to the Configuration menu.

Function of the individual icons:
Bottle turning device:

This function indicator shows that the device has been
configured for operation with the optional bottle
turning device.
The Bottle Turning Device dialog box can also be
accessed directly by touching the Bottle Turning
Device icon in the main menu.

Low water level:

This error indicator shows that the water level sensor
has detected a low water level.

Low humidity:

This function indicator shows that the relative
humidity in the work space has been lowered from
approx. 93% to approx. 90%.

Keypad lock:

This function indicator shows that the keypad lock has
been activated so that currently the settings cannot be
changed.

Overtemperature:

This error indicator shows that the device control has
activated the overtemperature protection and has
switched to backup control.

Fig. 59: Icon description overview

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Gas cylinder empty:

Error message, indicating, that the filling level of one
or of serveral gas cylinders is too low to ensure regular
gas supply.
The monitoring function is available only for device
versions with the optional gas monitoring system.

Request service:

This indicator shows that the routine service is due.
The display of the icon is controlled by the time entry
in the R

EMINDER INTERVAL dialog box and it appears

after the reminder message has been confirmed.

Gas cylinder filling level display (optional):

If the device is equipped with the optional gas
monitoring system the icons [1] for gas cylinder A and
gas cylinder B appear in the corresponding CO

2

display / O

2

display. The icons indicate the gas
cylinder filling level (full / empty).
The icon with the blue frame indicates the bottle not in
use that will be switched over to continue the supply.

• Manual changeover to a full gas cylinder is
possible.
> Press the cylinder icon with the blue frame.

or

• changeover can occur automatically, if:
> the cylinder pressure drops below 0.6 bar.

After a manual or automatic changeover of the gas
supply, another changeover is inhibited for 30
seconds. It takes approximately 2 minutes before the
filling level of the new cylinder has been determined
and can be displayed.
The gas monitoring system monitors the filling state of
the two connected gas cylinders.
If one cylinder is empty:

• no audible alarm sounds but an error message is
displayed,

• an entry is written into the event list.

If both bottles are empty:

• an audible alarm sounds and the alarm relay swit­ches,

• an error message is displayed,

• an entry is written into the error table,

• an entry is written into the event list.

Fig. 60: Gas monitoring icons

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Cylinder changeover:

A manual or automatic changeover of a gas
cylinder is entered into the event display.

7.11.5 Enabling/disabling the keypad lock

This input dialog box allows the keypad lock to be
enabled or disabled. At the factory, the keypad lock is
preset to the standard code 0000.

1. Enter the 4-digit code using the keypad. The input
appears encrypted in the display [1].

2. To delete complete incorrect input:

> Press the D

ELETE key [2].

3. To break off the input:

> Press the B

ACK key[4].

The system returns to the Configuration menu.

4. To confirm the input:

> Press the E

NTER key [3].

> The system returns to the Configuration menu.

Changing an existing code:

The currently valid code can be redifined in
the K

EYPAD LOCK CODE dialog box of the

S

ETTINGS/SETUP menu. (see chap. 7.11.3‚

pg. 85).

Resetting the code:

If the keypad lock code is no longer
available, the code must be reset to the
standard code by the Technical Support of
Thermo Fisher Scientific.

Fig. 61: Enabling/disabling the keypad lock

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7.11.6 Software versions

This menu shows the unit software versions in the
display [1].

• To exit the display:

> Press the E

ND key [2].

> The system returns to the Configuration menu.

Fig. 62: Software versions

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7.12 Scaling the trend display

The trend display of the three control loops:

• Temperature,

•CO

2

,

•O

2

,

can be scaled to two different versions.

1. Full screen display

> Press the display, for the value required.
> press the S

HOW TREND ICON [1].

2. To display an enlarged section:
> Open a rectangular section [2] in the desired

area of the diagram using a finger or a pen. The
size of the rectangle is determined by dragging
a diagonal from the start (press onto screen at
left of upper diagram frame) to the end (release
at right of lower diagram frame).

> Press any position within the marked rectangle

area [2]. The section is now enlarged.

> This process can be repeated any number of

times until the section is displayed in the desi­red enlargement or until the maximal enlarge­ment level (max. 30 event logger elements, i.e.
30 min. process at a logging cycle of 60
seconds).

> During zoom mode the trend display can be

scrolled forward and back.

3. To return to the total trend:
> Open rectangle over a small diagram section

and press any position outside the marked
area.

4. To exit the trend display:

> Press the E

ND key [3].

> The system returns to the main menu.

Logging cycle:

The time interval of the data logging cycle
can be redefined in the I

NTERVAL TIME EVENT

L

OGGER dialog box (see chap. 7.11.2‚

pg. 83).

Fig. 63: Trend display scaling

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7.13 Error messages

The error detection system is an integral element of
the device-internal control system. It monitors the
control loops and their sensors. If an error is detected
in the system, the alarm relay switches and issues the
following signals and messages:

• An audible alarm sounds,

• a flashing warning triangle [1] is displayed in the
main menu; the value indicators are no longer
updated,

• the detected error is listed in the error table,

• the event is entered into the event display.

7.13.1 Response to an error message event

If the alarm relay was switched due to a user action,
the switching state can be reset by accepting the error
message (e.g. for the manual cancellation of the
contra-con decontamination routine).
If the alarm relay was switched due to a techical
defect, the switching state remains active until the
defect has been corrected (e.g. low water level in work
space).

1. To accept the failure message:

> Press any position on the touchscreen.
> The Error dialog box [2] apppears and the

detected error is displayed,

> the audible alarm is switched off.

2. To exit the error display:

> Press the E

ND key [3].

> The error message disappears.

7.13.2 Troubleshooting

The error tables indicate source of error, cause of
error, and possible corrections.
For any communication with the Technical Support of
Thermo Fisher Scientific, please have the device data
ready.

Fig. 64: Event error messages

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Error message overview:

Control

loop

Error message Cause Repair Alarm relay Buzzer

System

Device door open
too long

Doors ope n for more than 10
minutes.

Close device doors x x

Failure display

Communication between
display and main board faulty

Contact Service when failure
is recuring

xx

Failure EEPROM
main board

EEPROM on main board is
faulty.

Contact Service x x

Failure
communication
mainboard

Communication between
display and main board faulty
*1)

Contact Service when failure
is recuring

System - Failure
data logger

Failure during recording data
logger. Device is still
working.

Reset device to cancel failure.
When failure is recuring
contact Service.

x

Failure contra-con Failure in contra-con routine

Reset device to cancel failure.
When failure is recuring
contact Service.

xx

System - Power
down during contra

-

con

Power failure during contra­con routine

Reset device and start contra­con again.

xx

Failure upon auto­start

Failure during auto-start
routine

Reset auto-start. When failure
is recuring contact Se rv ice.

xx

System - Safety
circuit active

Temperature signal
plausibility doubtful *2)

Reset device. When failure is
listed several times, contact
Service.

Failure
bottle turning
device

Communication between
bottle turning device and
main board faulty

Contact Service x x

Tempe­rature

Sensor breakage

Detected value is out of
limit.

Contact Service x x

Actual value abov e

Act. val. > nom. val. + 1°C
*3)

Do not exceed ambient
temperature limit

xx

Actual value below

Act. val. < nom. val. - 1°C
*4)

Contact Service x x

Calibration values
too high / too low

Temperature max.
adjustment value exceeded

Contact Service x x

Actual value
plausibility
doubtful

Temperature signal
plausibility doubtful

Contact Service x x

Failure
communication\

Measuring cell cannot
commuicate with mainboard

Contact Service x x

*1) Error message will be displayed, but not entered in error table.
*2) Error message will be displayed, but not entered in error table.

*4) After changing the set value, error time will be set on 159 min., after door opening on 45 min. ( O

2

on 159 min.).

*3) If failure is detected, a special sample protection controlling will be enabled. To indicate the start of this routine,
the icon overtemperature is displayed. The routine will will be disabled after a power reset.

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Error message overview:

Control

loop

Error message Cause Repair Alarm relay Buzzer

Tempe­ratur

Failure LM75

LM75 sensor cannot not
communicate with
mainboard

Contact Service x x

Failure EEPROM
sensor

NV-RAM sensor faulty Contact Service x x

CO

2

Sensor breakage

Detected value is out of
limit.

Reset auto-start. When failure
is recuring contact Se rv ice.

xx

Actual value abov e

Act. val. > nom. val. + 1%
*4)

Check gas supply. Reduce
prepressure to 1 bar max .

xx

Actual value below

Act. val. < nom. val. - 1%
*4)

Check gas supply. Change
gas cylinder. Raise
prepressure to 1 bar max ..
Check supply line to device.

xx

Calibration values
too high / too low

Max. adjustment value
exceeded for CO

2

Contact Service x x

Failure
communication

Measuring cell cannot
commuicate with mainboard

Contact Service x x

Failure gas
cylinder
changeover switch

Gas cylinder changeover
switch cannot commuicate
with mainboard

Contact Service x x

No gas Both CO2 cylinders empty

Replace one or both O2
cylinders.

xx

Gas cylinder A
empty

Gas cylinder A is empty. Replace gas cylinder A.

Gas cylinder B
empty

Gas cylinder B is empty. Replace gas cylinder B.

Failure EEPROM
sensor

NV-RAM sensor error. Contact Se rvice x x

O

2

Sensor breakage

Detected value is out of
limit.

Contact Service x x

Actual value abov e

Act. val. > nom. val. + 1%
*4)

Check gas supply. Reduce
prepressure to 1 bar max .

xx

Actual value below

Act. val. < nom. val. - 1%
*4)

Check gas supply. Change
gas cylinder. Raise
prepressure to 1 bar max ..
Check supply line to device.

xx

Failure
communication

O

2

sensor cannot

commuicate with mainboard

Contact Service x x

*4) After changing the set value, error time will b e set on 159 min., after door opening on 45 min. ( O2 on 159 min.).

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Error message overview:

Control

loop

Error message Cause Repair Alarm relay Buzzer

O

2

Failure gas cylinder
changeover switch

Gas cylinder changeover
switch cannot commuicate
with mainboard

Contact Service x x

No gas Both CO

2

cylinders empty

Replace one or both O

2

cylinders.

xx

Gas cylinder A
empty

Gas cylinder A is empty. Replace gas cylinder A.

Gas cylinder B
empty

Gas cylinder B is empty. Replace gas cylinder B.

rH No wate r

Water level in the incubator
under

Fill in water or if the auto-start
routine is to be run dry
(without water), the water
level sensor can be switched
off.

xx

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8 Shut-down

8.1 Shutting the device down

Contamination hazard!

If the work space surfaces are contaminated, germs can be
transferred to the environment of the device.
In case of a shut-down, the device must be decontaminated!

1. Remove the culture containers and all accessories from the work
space.

2. Pump the water out off the water reservoir (see chap. 9).

3. Fill in 350 ml water and start the contra- con decontamination routine.

4. Turn the device off using the power switch when the contra-con
decontamination routine has been completed.

5. Unplug the power connector and protect it against accidental
reconnection.

6. Close the CO

2/O2/N2

supply system shut-off valves.

7. Disconnect the gas pressure hoses from the sleeve at the rear of the
device.

8. Clean the work space and wipe the device dry.

9. Until the device is shut down, the work space must be continuously
ventilated. Leave the glass door and the outer door open and secure
them in this state.

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9 Cleaning and disinfection

9.1 Cleaning

Incompatible cleaners!

Some device components are made of plastic. Solvents can
dissolve plastics. Strong acids or caustic solutions can cause to
become brittle of the plastic.
For cleaning plastic components and surfaces, do not use
solvents that contain hydrocarbons, solvents with an alcohol
content of more than 10% or strong acids or caustic solutions.

Moisture-sensitive components!

Do not spray cleaning agent onto the touchscreen and the
control box at the rear of the device. When wiping the device
clean, always make sure that moisture does not enter into
these components.

Exterior surfaces Cleaning exterior surfaces:

1. Remove dirt residues and depositions thoroughly using a solution of
water and commercial detergent.

2. Wipe the surfaces clean using a clean cloth and clear water.

3. Then, wipe the surfaces dry using a clean cloth.

Cleaning display:

Moisture-sensitive display!

Do not spray or wipe the display with cleaner.

• Clean display using a dry cloth of 100% micro fiber!