Thermo Scientific GENESYS 30, GENESYS 50, GENESYS 180, GENESYS 40, GENESYS 140 Instruction Manual

UV-Visible

GENESYS Family
Spectrophotometers

User Guide

269-331300 Revision A February 2018

© 2018 Thermo Fisher Scientific Inc. All rights reserved.

Eppendorf and UVette are either trademarks or registred trademarks of Eppendorf AG aktiengesellschaft.
BrandTech is a trademark of BrandTech Scientific, Inc. Beckman is a trademark of BECKMAN COULTER,
INC. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error­free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.

For Research Use Only. This instrument or accessory is not a medical device and is not intended to be used
for the prevention, diagnosis, treatment or cure of disease.

WARNING Avoid an explosion or fire hazard. This instrument or accessory is not
designed for use in an explosive atmosphere.

C

Contents

Chapter 1 GENESYS Spectrophotometers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Site Preparation and Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Operating Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Spectrophotometer Basics - GENESYS 40 and GENESYS 50. . . . . . . . . . . . . . . 3

Sample Compartment - GENESYS 40 and GENESYS 50 . . . . . . . . . . . . . . . 3

Single Cell Holder - GENESYS 40 and GENESYS 50 . . . . . . . . . . . . . . . . . . 4

Optional Sample Holders - GENESYS 40 and 50. . . . . . . . . . . . . . . . . . . . . . 5

Cell Holder Replacement - GENESYS 40 and 50 . . . . . . . . . . . . . . . . . . . . . . 6

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180

and BioMate 160. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Sample Compartment - GENESYS 140, GENESYS 150, GENESYS 180

& BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Single Cell Holder - GENESYS 140, GENESYS 150, GENESYS 180 &

BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Optional Single Sample Holders - GENESYS 140, GENESYS 150,

GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Disposable 50 mL Microcell Holder – GENESYS 140, GENESYS 150,

GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Beckman Cell Holder – GENESYS 140, GENESYS 150,

GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Peltier Thermostatted Single Cell Holder – GENESYS 140,

GENESYS 150, GENESYS 180 and BioMate 160 . . . . . . . . . . . . . . . . . . 11

Sipper Accessory - GENESYS 140, GENESYS 150, GENESYS 180

and BioMate 160 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Fiber Optic Coupler and Probe (UV Vis systems only) . . . . . . . . . . . . . . . . . 21

Spectrophotometer Basics – All Models. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Halogen Lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Lid Removal and Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Chapter 2 GENESYS On-board Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

GENESYS On-board Software Home Screen and Navigation. . . . . . . . . . . . 33

Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Thermo Scientific GENEYS Family Spectrophotometers i

Contents

Viewing Saved Experiment Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Application Home. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Creating A New Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Experiment Execution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Data Analysis and Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Live Display Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Fixed Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Scan Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Quant Application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Calibrated and Uncalibrated Quant Methods . . . . . . . . . . . . . . . . . . . . . . . . 47

Cell Changer Setup in Quant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

C-Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Kinetics Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Importing Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Exporting Data and Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Performance Verification Tests and Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Running Performance Verification Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Customized Performance Verification Tests . . . . . . . . . . . . . . . . . . . . . . . . . 54

Customizing Stray Light Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Customizing Wavelength Accuracy Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Customizing Photometric Accuracy Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Accessory Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Cell Changer Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Sipper Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Calibrated Sipper. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Calibrating the Sipper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Custom Workflows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Peltier Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Accessory Configuration in Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

SmartStart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Configuring and Switching to SmartStart Mode . . . . . . . . . . . . . . . . . . . . . . 75

Exiting SmartStart Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Connecting to VISIONlite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Enable the VISIONlite Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Printer Setup and Wi-Fi Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Setting Up USB Printer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Setting Up Thermal Printer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Configuring Network Paths. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

ii GENEYS Family Spectrophotometers Thermo Scientific

Contents

Chapter 4 BioMate 160 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87

OD600 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

dsDNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Protein BCA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Protein Bradford . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Protein Lowry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Protein Pierce 660 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

Protein Rapid Gold BCA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

Thermo Scientific GENEYS Family Spectrophotometers iii

Contents

iv GENEYS Family Spectrophotometers Thermo Scientific

1

GENESYS Spectrophotometers

This user guide applies to the following spectrophotometers:

• Intermediate capability models – the Thermo Scientific™ GENESYS™ 50 platform – with
sample compartments configured for single samples only:

– GENESYS™ 40 Vis

– GENESYS 50 UV-Vis

• Advanced capability models – the GENESYS™ 150 platform – with sample
compartments configured for:

–single samples

– multi-cell changer option

– Peltier thermostatted cell holder option

– fiber optic probe option

• GENESYS™ 140 Vis

• GENESYS 150 UV-Vis

• GENESYS™ 180 UV-Vis

•BioMate™ 160

Note There is also a GENESYS™ 30 Vis that shares the sample compartment
configuration of the GENESYS 50 platform but offers a user experience with a tactile
rubber keypad, high resolution non-touch color 5-inch screen and simplified software.
The GENESYS 30 has a separate dedicated user guide

Thermo Scientific GENEYS Family Spectrophotometers 1

1

GENESYS Spectrophotometers

Considerations

Considerations

This document uses the following conditions:

DANGER Indicates a hazardous situation which, if not avoided, will result in death or
serious injury.

WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.

CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.

NOTICE Follow instructions with this label to avoid damaging the system hardware or
losing data.

Note Contains helpful supplementary information.

Site Preparation and Safety

Before using the system, read the site preparation and safety manual on the documentation
media provided. Always follow the safety precautions in that manual and in this document
when using the system.

Operating Precautions

WARNING Do not operate this system without following the safety precautions described

in this manual and the documentation that came with your system.

The spectrophotometer contains precise optical components. Handle it carefully and follow
these precautions.

• Do not allow moisture to leak into the instrument interior

• Wipe off spilled chemicals immediately

• Do not drop the instrument

• Protect the instrument from mechanical shock

• Protect the instrument from dust

2 GENEYS Family Spectrophotometers Thermo Scientific

1

Lift here

Closure magnet

Light beam path

Detector lens

Monochromator
window

Tray alignment
posts

Drain

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 40 and GENESYS 50

Spectrophotometer Basics - GENESYS 40 and GENESYS 50

Remove all tape from the exterior of the instrument and inside the sample compartment.

Sample Compartment - GENESYS 40 and GENESYS 50

Thermo Scientific GENEYS Family Spectrophotometers 3

High durability constant torque hinges hold
the lid at any angle

Window and lens protect interior optics from spills and
vapors.

Magnet at front holds lid closed to exclude
light when door is lowered

Sample holder tray aligns to posts on baseplate.

Excess spills drain to benchtop.

1

Locater pin

Magnets

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 40 and GENESYS 50

Single Cell Holder - GENESYS 40 and GENESYS 50

Standard 10 mm cuvette holder Underside of a single cell holder

Tray features

• Able to contain spills up to 150 mL

• Can be removed by pulling up on the cell holder

• Can be washed in the sink or a dishwasher - dry promptly!

NOTICE

• Clean the tray with water and mild detergent. Ethanol and iso-propyl alcohol can be
used if necessary but do not soak the tray in alcohols.

• Do not allow acetone, chlorocarbons or other aggressive organic solvents to contact
the tray. The PC-ABS plastic will soften and discolor.

Removal – grasp cell holder and lift up and forward

Insertion – allow front magnet to engage. Lower cell holder into place, allowing back
magnet to guide and engage

4 GENEYS Family Spectrophotometers Thermo Scientific

Optional Sample Holders - GENESYS 40 and 50

Cell holder trays equipped to position other kinds of cells and samples are available. They
insert and remove in the same way as the standard cell holder.

Te st tu b e h o ld e r

Tall test tube adapter

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 40 and GENESYS 50

Long path rectangular cell
holder

Long path cylindrical cell
holder

Filter holder

Accessory kit for hose
entry/exit

Thermo Scientific GENEYS Family Spectrophotometers 5

1

Lift here to open

Lid hold-down
magnet

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Cell Holder Replacement - GENESYS 40 and 50

Thermostatted cell holder and Adjustable filter holder accessories are supplied without a tray.

Loosen the captive screw at the base of the cell holder to remove it. Attach the new sample
holder in the same way.

Note The Accessory Kit for Hose Entry/Exit is required to support use of the
thermostatted cell holder. See Lid Removal and Replacement for additional installation
instructions.

Spectrophotometer Basics - GENESYS 140, GENESYS 150,
GENESYS 180 and BioMate 160

Remove all tape from the exterior of the instrument and inside the sample compartment.

Sample Compartment - GENESYS 140, GENESYS 150, GENESYS 180 & BioMate 160

High durability constant torque hinges hold
the lid at any angle

Magnet at front holds lid closed to exclude
light when door is lowered

6 GENEYS Family Spectrophotometers Thermo Scientific

1

Alignment
posts

Opto-sensor

Drain

Detector lens

Monochromator
window

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Align arrow on sample holder base with
arrow on sample compartment and groove
on sample compartment wall.

Window and lens protect interior optics
from spills and vapors.

Sample holder aligns to posts on baseplate
and groove.

Excess spills drain to benchtop. Opto-sensor
is used for positioning cell changer.

Thermo Scientific GENEYS Family Spectrophotometers 7

1

Locater pin

Ring magnet

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Single Cell Holder - GENESYS 140, GENESYS 150, GENESYS 180 & BioMate 160

Standard 10 mm cuvette holder

NOTICE

• Clean cell holders by wiping with a solution of mild detergent. Ethanol and isopropyl
alcohol can be used if necessary but do not submerge the cell holder in liquid.

• Do not allow acetone, chlorocarbons or other aggressive organic solvents to contact
the cell holder or base. The PC-ABS plastic will soften and discolor.

Removal—grasp blue knob and lift straight upInsertion—align arrow on left side of sample

compartment with arrow on sample holder
accessory. Lower cell holder into place.
Magnet holds accessory down. Pin under
accessory base aligns cell holder in beam and
prevents rotation.

8 GENEYS Family Spectrophotometers Thermo Scientific

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Optional Single Sample Holders - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Cell holder trays equipped to position other kinds of cells and samples are available. They
insert and remove in the same way as the standard cell holder.

Long path rectangular cell holder

Long path cylindrical cell holder

Filter holder

Disposable 50 μL Microcell Holder

Beckman cell holder

Peltier Thermostatted cell holder

Note Water thermostatted cell holders are not supported on this platform. See the Peltier
Thermostatted Cell Holder accessory.

Thermo Scientific GENEYS Family Spectrophotometers 9

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Disposable 50 mL Microcell Holder – GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Disposable plastic microcells with 50 μL chambers located at a Z-height of 8.5 mm and
centered on the cell are supported by this accessory. At time of writing of this user guide, the
Eppendorf™ UVette™ is the only brand supported, but see the insert with the accessory for
updates. Cuvettes made by BrandTech™ are not supported because of poor manufacturing
reproducibility in these cells.

Note Plastic microcells are not as transparent as quartz, especially at very short
wavelengths. The combination of absorption by the cell and masking of the
spectrophotometer beam required to restrict it to the 2.5 mm tall cell window reduces the
energy passing through these cells. Photometric performance will be lower with these cells
than with full size quartz cells.

Beckman Cell Holder – GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Beckman™ pioneered the use of a special type of microcell with volumes of 100 μL or less.
These very short cells require a special holder to accommodate them at the appropriate
Z-height for the cell and to mask off part of the spectrophotometer beam to ensure that light
only passes though the sample, and not over it.

10 GENEYS Family Spectrophotometers Thermo Scientific

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Peltier Thermostatted Single Cell Holder – GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

The accessory holds a single cell at a temperature between 20° and 60 °C with a precision of
≤0.2 °C and an absolute accuracy of ±0.5 °C.

Note The specification for absolute accuracy is limited by the accuracy of the
thermocouple used in construction of the device. ±0.3° C is the specification of a superior
quality thermocouple. Beyond this specification the cost of the thermocouple becomes
exponentially higher and use of a calibrated thermocouple becomes a necessity if
exceptionally high accuracy is a requirement.

In our tests, using a calibrated thermocouple probe, we generally find the temperature of the
solution in the cuvette to fall within ±0.3 °C of the programmed absolute temperature.

The temperature measured and reported by the device is the temperature of the metal
cell-holder block. The temperature of stirred liquid in a full size quartz cell will reach the same
temperature as the cell holder block after approximately 6 minutes when heating from 23 °C
to 35 °C. This is only a guide. Performance will vary if:

• The initial temperature is different

• The final temperature is below room temperature

• The cuvette is not stirred

• The cuvette is not made of glass or quartz

• The cuvette is a micro-cuvette

Thermo Scientific GENEYS Family Spectrophotometers 11

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

The experimental parameters for controlling the Peltier thermostatted cell holder include an
equilibration time. This is a “wait time” between when the cell holder block reaches the target
temperature and when the absorbance measurement is made. We strongly recommend that
you place the probe of a digital thermometer or thermocouple of sufficient accuracy and
precision in the liquid in a cell under what will be normal experimental conditions, and bring
a quantity of appropriate matrix (solvent or buffer) to temperature in the cell holder while
monitoring the temperature to determine performance under your experimental conditions.
A white paper describing important considerations and limitations in thermostatting
experiments is included with this documentation set. Refer to Introduction to Temperature
Control for additional details.

In brief, however:

• Quartz conducts heat six times faster than plastic – samples in quartz cells come to
temperature much faster than they do in plastic cells.

• A full size cell takes approximately 66% more time to reach equilibrium temperature if it
is not stirred.

Installing the Accessory

Align white arrows as shown.

• Liquid in unstirred plastic cells did not heat to within 0.6 °C of the cell holder block
temperature when heating in our experiments.

• Plastic microcells and semi-microcells have significantly reduced surface contact with the
walls of the cell holder block compared to full size plastic cells or glass/quartz reduced
volume cells. If using a reduced volume plastic cell it is essential that you perform some
experiments to determine the appropriate equilibration time setting.

12 GENEYS Family Spectrophotometers Thermo Scientific

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Lower the cell holder into the sample compartment so that the V engages with the groove on
the side of the sample compartment and the hold-down magnet engages

Connect cables as shown.

• The power supply is identical to the instrument power supply

• Operate this accessory with the sample compartment lid open

• Do not close the sample compartment lid while the accessory is running

• Optional – the sample compartment lid can be removed while using this accessory

See “Peltier Configuration” on page 69 of this guide for instructions on configuring your
method to use this accessory.

Thermo Scientific GENEYS Family Spectrophotometers 13

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Sipper Accessory - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Introduction to Sipper Use

A detailed introduction to sippers and how they can be used to get the best results in different
situations is provided in a separate document (English language only) included in the
documentation media with this user guide.

Components of the Sipper System

Complete sipper systems come with the following components

Item Photograph

Sipper pump

Sample compartment insert with
gooseneck

14 GENEYS Family Spectrophotometers Thermo Scientific

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

3 m PTFE transfer tubing

1 Flow cell, 160 µL volume
with screw connector set

Smaller volume flow cells are available but the smaller window size cuts off some of the spec­trophotometer beam, reducing photometric performance. If the absorbance values measured
are below 1.0, however, this will not make a noticeable impact on data quality.

Connector package

The connectors supplied are designed for use with the # 16 tubing supplied. They can also
be used with smaller # 14 tubing with extra effort. To use larger # 25 tubing please purchase
a larger connector separately.

Item Photograph

30 cm Silicone pump tubing
(#16 size – 1.6 mm wall,

1.6 mm inside diameter)

1

GENESYS Spectrophotometers

Thermo Scientific GENEYS Family Spectrophotometers 15

1



Inlet marker

Attach long
connector to inlet

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Compatibility of the GENESYS Sipper with tubing sold by us for use with
spectrophotometer sippers.

Material / Size #13 #14 #16 #25

Silicone

C-Flex

Viton

ChemDurance Bio

a

Chemdurance Bio tubing is suitable for use with the Smart Sipper
for Evolution spectrophotometers

a

Connecting the Sipper Tubing

1. Connect screw connectors to flow cell.

16 GENEYS Family Spectrophotometers Thermo Scientific

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

2. Attach the gooseneck tube.

3. Lower the cell holder into the sample compartment so that the V engages with the groove on the side of the sample compartment and the hold-down magnet engages.

4. Position sipper pump and connect cables.

Note The power supply for the pump is identical to the power supply for the
spectrophotometer. There is no risk or danger to connecting “the wrong one”.

Thermo Scientific GENEYS Family Spectrophotometers 17

1

Pump head connection taking
silicone tubing to waste

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

5. Decide where the waste container will be placed.

• If very close to the pump, run the pump tubing directly to the waste container.

6. If farther, run (less expensive) PTFE transfer tubing from the pump-head tubing to the waste container.

7. Determine the length of pump tubing required for your configuration. Minimum recommended length of pump tubing is 10 cm.

8. Cut a suitable length of pump head tubing and lay it on the bench in front of the pump.

9. Place the flow cell in the cell holder with the windows in the beam (facing to the front and back).

18 GENEYS Family Spectrophotometers Thermo Scientific

1

Zip tie

Turn right to tighten

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

10. Route the PTFE cell connector tubing from the inlet (long connector) through the gooseneck.

Refer to separate Introduction to Sipper Use for detailed information.

11. Route the PTFE cell connector tubing from the outlet (short connector) to the pump.

12. Trim all cell connector tubing to an appropriate length for your use scenario.

13. Attach the pump-head tubing to the PTFE tubing using the couplers.

Secure the connections with zip-ties if using high pump speeds or tubing larger than the
size supplied.

14. Install the pump-head tubing into the pump head as shown.

15. Test the system for leaks.

Use the table on the pump to choose a pump speed suitable to the volume that you wish
to pump in your procedure.

Note Accuracy of the volume delivered decreases as pumping speed and tubing size
increase.

Thermo Scientific GENEYS Family Spectrophotometers 19

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Program a custom sequence to sip at the chosen speed for 60 seconds, or longer if
necessary. Place the inlet tube in a beaker of clean solvent and pump for long enough to
completely fill the system with solvent and deliver it to the waste container.

Repeat the sip sequence while observing the PTFE tubing between the flow cell and the
pump. There should be no bubbles in the tubing. If there are, check the tubing
connections to the flow cell.

Remove the flow cell from the cell holder and check it for solvent leaks.

Check the connections between the PTFE tubing and the pump-head tubing for solvent
leaks.

16. Follow the process to calibrate the sipper.

Failure to deliver a consistent volume can be an indicator of an air leak at the connection
between the cell outlet tubing and the pump-head tubing.

Controlling the Sipper from Software

Two sipper control modes:

1. Uncalibrated – Define pump time at all stages.

2. Calibrated – Define pump volume at each pumping stage.

Sipper parameters are stored in the experimental method. Sipper control is supported only in
Quant, Fixed and Scan modes.

See “Sipper Configuration” on page 63 for instructions on programming the sipper.

Sipper setup controls

Selection Definition

Sip Pump in the forward direction

(from the inlet tube towards the
flow cell and pump)

Settle Time to wait for the liquid to stop

moving before making a
measurement

Air gap Pump air in the forward direction

to push liquid through the system

Rinse Pump rinse solution in the forward

direction

Return Pump in the reverse direction (from

the pump and flow cell towards the
inlet tube)

20 GENEYS Family Spectrophotometers Thermo Scientific

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Selection Definition

Measure Record the absorbance

Prompt Display an on-screen prompt to tell

the user what to place at inlet tube
for the next step in the sequence

Uncalibrated vs. calibrated sipper operation

Customers whose existing SOPs are based on a prior sipper system that did not support
calibration for volume pumped can continue to use time-based pumping in uncalibrated
methods.

In general, calibrating the system for volume delivered is the preferred method. Once
calibrated, the system will pump the specified volume at each step.

Fiber Optic Coupler and Probe (UV Vis systems only)

1

GENESYS Spectrophotometers

NOTICE Fiber optic cables can suffer damage when bent with too small a radius. A small

radius can also lead to light loss and reduction of performance even if it does not lead to
physical damage. The recommended minimum bend radius when working is 20cm.

Note The fiber optic probes that we supply are equipped with a shorter than normal
collar at the SMA connector. This helps to maximize the bend radius of the input fiber.
Probes purchased from other vendors will generally have a 25 mm long collar at the SMA
connector. This will make them difficult to install and may result in an unacceptably small
bend radius to the fiber as it exits the collar. If you wish to use a third-party probe, we
recommend that you specify a 13 mm collar.

Assembly

Assemble the fibers to the coupler as shown in the photograph.

1. Loosen the three thumb screws on the top cover and remove it from the coupler.

Thermo Scientific GENEYS Family Spectrophotometers 21

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

2. Remove the protective plastic covers from the ends of the fibers.

Save these covers and re-attach them if you remove the probe.

3. Slide the tips of the SMA connector into the receivers and tighten the nuts hand tight.

Do not use tools to tighten the nuts further.

4. Loosen the hold-down thumb-screw, place the fibers under it, and tighten it down to hold the fibers in place.

Do not over-tighten. Be sure to route the cables as shown in the photograph above. This
routing pattern maximizes the bend radius.

Note The routing distance for the input and output fibers is slightly different so there
will be a small excess loop between the junction nut and the coupler. This is normal
and correct.

5. Re-attach the top cover to the coupler.

6. Remove the black rubber cover from the pick-up lens. Be careful not to touch the lens with your fingers.

7. Lower the coupler into the sample compartment with the white arrows aligned.

8. Ensure that the probe tip is tight. It screws on and off in the expected way.

22 GENEYS Family Spectrophotometers Thermo Scientific

1

Configuration 1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

Operation

The recommended operation wavelength range is from 220 nm to 1100 nm. Although the
fibers are solarization resistant they can suffer cumulative damage (they become opaque) when
used at very short wavelengths. Damage to the fibers due to solarization is not covered under
warranty.

Two configurations are recommended:

1. Place the probe in a clamp and bring the container with the solution up to it.

2. Hold the probe in your hand and place it into the container with the solution.

Measure the blank solution first and then measure the sample(s) – exactly as you would if
you were making measurements with cuvettes.

It is VERY important to avoid having an air bubble in the tip when making a
measurement. Air bubbles will result in completely incorrect measurement values. Tap the
probe with your finger to knock air bubbles out if using configuration 1. “Shake” the
probe back and forth quickly and/or tap it with your finger to knock air bubbles loose if
using configuration 2. Do not tap the probe on the side of the container. This could
damage the tip or the container.

Probe Tips with Different Pathlengths

While tips with other pathlengths can be purchased for the dip probe we do not recommend
their use with the GENESYS systems and have not tested other configurations. Longer
pathlengths typically lead to greater light-loss in the tip, reducing photometric range. Shorter
pathlengths are more prone to trapping air bubbles in the tip and it is more difficult to shake
them loose.

Performance

Fiber optic couplers and probes “cost” light in a spectrophotometer system. Having less light
intensity in the system means not being able to deliver the same photometric performance
with the fiber probe system that the instrument can deliver with cuvettes in a dark sample
compartment. Do not perform the performance verification tests when using the fiber optic
probe system.

Thermo Scientific GENEYS Family Spectrophotometers 23

1

GENESYS Spectrophotometers

Spectrophotometer Basics - GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160

As a guide, in our tests, we found the system to very resistant to room lighting conditions and
to deliver good linearity to approx. 1.8A and an upper limit of measurement of 2.2A. If
performing quantitative analysis with this accessory and measuring above 1A we recommend
that users choose a quadratic (second order) fit for the curve and work in an operational range
below 1.8A.

Troubleshooting

Problem Cause Solution

Readings are unexpectedly high or low
with no consistency to the error

All readings are “off” by a uniform
amount

Change in photometric performance –
depressed ABS readings or maximum
ABS reading

Air bubbles in the tip during
sample measurement

Inspect the gap in the tip prior to
making a measurement. Take steps to
remove any bubbles.

Inconsistent small bend radius Use a large bend radius and keep it

consistent

Air bubble in the tip during blank
measurement

Inspect the gap in the tip prior to
making the blank measurement. Take
steps to remove any bubbles.

Bend radius too small Configure your experimental setup to

use a large and consistent bend radius

Damaged optical fiber Follow the recommendations above.

Record a blank and scan from
1100 nm to 220 nm with deionized
water. Save the spectrum. The
spectrum should be essentially flat
with < ±0.05A baseline noise. Large
spikes or discontinuities are common
signs of fiber damage.

Try using a different probe if you have
one available.

Contact Us

Probe does not work at all – no signal Cover over pick-up lens on coupler Remove the lens cover.

Broken fiber Replace the probe.

Tip loose, damaged or missing Remove and inspect the tip. Ensure

that the mirror is present and clean.
Attach the tip and re-test the system.

Coupler not inserted correctly Remove and re-insert the coupler

SMA connector not properly
secured

Remove and re-attach the SMA
connectors inside the fiber coupler.
Ensure that the nuts are not cross
threaded.

24 GENEYS Family Spectrophotometers Thermo Scientific

Spectrophotometer Basics – All Models

On/Off Accessory

connector

USB-A
ports

Network/Ethernet
port

Electrical Connections

• Power toggle switch

• 12V DC—connect the cable from the power supply here

• Accessory connector—connect the data cable from the Peltier Thermostatted Cell Holder
or Sipper Pump (optional accessories) here

• USB-A ports—see Optional Accessories below

1

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

Optional Accessories

• Network/Ethernet port—connect a standard Ethernet (RJ45-RJ45) cable between this
port and a network port to communicate with the building network

A single USB-A port is located on the front (GENESYS 40 or GENESYS 50) or the side
(GENESYS 140, GENESYS 150, GENESYS 180 and BioMate 160) and a pair of USB-A
ports is located on the main connector panel (pictured).

The USB ports support the following peripheral devices:

• Special cable to PC to run the instrument from VISIONlite software

• Standard keyboard

• Mouse or touchpad

• Dongle for wireless keyboard/mouse

•Printer

• USB flash memory device

• Barcode reader

Thermo Scientific GENEYS Family Spectrophotometers 25

1

Finger hold

Printer housing

Lift

1 2

3

4

5

6

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

Printer

1. Remove the printer housing cover.

2. Load paper into the optional printer.

Use the finger hold, pull towards you and lift.

26 GENEYS Family Spectrophotometers Thermo Scientific

3. Insert printer into GENESYS spectrophotometer.

Guide rails

Guide rails

Connector

Connector

Bottom of printer

Release lever

Back of GENESYS

Serial Number

1

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

a. Align the guide rail on the printer with the guide rail on the GENESYS

spectrophotometer.

Thermo Scientific GENEYS Family Spectrophotometers 27

1

Slide forward Printer fully engaged

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

b. Push the printer forward until the connectors are fully connected.

You will hear a snap when the connectors have engaged properly.

28 GENEYS Family Spectrophotometers Thermo Scientific

Halogen Lamp

Lamp housing
connector

Lamp housing
guide rail

Lamp

connector

Lamp
guide rail

1 2

3

4

Bottom of lamp

Back right corner

The lamp source lifetime is approximately 1,000 hours.

 To replace the halogen lamp

1

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

Thermo Scientific GENEYS Family Spectrophotometers 29

GENESYS 40 shown. GENESYS 140 is similar.

1

1

2

3

4

5

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

WiFi Transmitter

1. Print to a wireless printer in the room.

2. Connect to a WiFi router.

NOTICE Prevent damage to the instrument. Remove the WiFi Transmitter from the
instrument and package it separately when shipping the instrument – for example to our
depot for service.

30 GENEYS Family Spectrophotometers Thermo Scientific

Lid Removal and Replacement

Loosen single Phillips/Cross screw as shown and lift lid assembly upwards. Attach new lid in
same way.

Lid removal may be required or desired when:

• Installing the Accessory Kit for Hose Entry/Exit on GENESYS 40 or GENESYS 50

1

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

• Using the Peltier Thermostatted Cell Holder accessory for the 100 series models

• Using the sipper accessory for the 100 series models

• Using the fiber optic probe accessory for the 100 series models

• Using the 8-cell changer for the 100 series models

• Replacing a damaged lid

Thermo Scientific GENEYS Family Spectrophotometers 31

1

GENESYS Spectrophotometers

Spectrophotometer Basics – All Models

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32 GENEYS Family Spectrophotometers Thermo Scientific

2

GENESYS On-board Software

Settings menu

to view
experiment data

to view
applications

GENESYS On-board Software Home Screen and Navigation

See Performance Verification Tests

and Reports

When an application is selected, the user is directed to an “Application Home” screen.

Thermo Scientific GENEYS Family Spectrophotometers 33

2

GENESYS On-board Software

Getting Started

Getting Started

On this screen, when available, existing methods can be loaded, new methods created or
existing methods modified or edited.

This section provides an overview of the basic operations within an application.

For detailed explanation of parameters within each application and advanced functionality,
please see the appropriate section in this manual.

Typically, the Thermo Scientific™ GENESYS™ On-board applications have the following
structure and workflows:

•Application Home

– View and select methods

– Export/Import/Data methods

– Select for Smart mode

•Setup

– Setup parameters

– Configure accessories

– Perform calibration for Quant

• Experiment Execution

– Perform Blank, begin measurement

– Edit sample names

– Select sample-specific actions, where applicable

• Data Analysis and Actions

– View and analyze data

–Export/print

– Save/discard

34 GENEYS Family Spectrophotometers Thermo Scientific

Viewing Saved Experiment Data

Data Viewer

Select experiment
data by name

2

GENESYS On-board Software

Viewing Saved Experiment Data

Thermo Scientific GENEYS Family Spectrophotometers 35

2

Select
method to
view details

‘3-dot’ menu
presents
more
method
options

Review and use method

Import from
network or USB

Sort by name

Select a
method as a
Smart Method.
See SmartStart

Edit to modify the selected
method

Create a copy of the
selected method and
modify parameters

See Importing Methods

GENESYS On-board Software

Viewing Saved Experiment Data

Application Home

This section uses the Scan application as an example. See the specific application section for
detailed explanation of parameters and options.

36 GENEYS Family Spectrophotometers Thermo Scientific

2

Long tap to select
multiple methods

Delete

Create new method

Edit method name

GENESYS On-board Software

Viewing Saved Experiment Data

Creating A New Method

Thermo Scientific GENEYS Family Spectrophotometers 37

2

All blue text and
buttons are
editable

See

Accessory
Setup

Blue save icon
indicates unsaved
changes.

Go to previous page

to save

All parameter
options are
presented in a
slide up menu

Follow prompts

to begin experiment

GENESYS On-board Software

Viewing Saved Experiment Data

Experiment Execution

38 GENEYS Family Spectrophotometers Thermo Scientific

2

Swipe right to view
method parameters

All measurements
are displayed

Print or export

Rename experiment
before saving (or use
default name)

All experiment data
must be saved.

GENESYS On-board Software

Viewing Saved Experiment Data

Example of a printed report. See Printer Setup and Wi-Fi

Printing for details.

Thermo Scientific GENEYS Family Spectrophotometers 39

2

Swipe cursors to points of
interest

View values

3-dot for advanced analysis

Scan samples have peak
pick options

Tap to edit sample name

Tap sample to highlight in
spectrum

Long tap to select multiple

for fine cursor
adjustment

to show/hide
cursor

GENESYS On-board Software

Viewing Saved Experiment Data

Data Analysis and Actions

40 GENEYS Family Spectrophotometers Thermo Scientific

2

Select Peak Pick

Peaks, Valleys
or both

Adjust ABS
scale to
specify
lower limit

Adjust Lambda cursors to specify
peak detect range

GENESYS On-board Software

Viewing Saved Experiment Data

Thermo Scientific GENEYS Family Spectrophotometers 41

See Scan Application for detailed
information on options and parameters.

2

The instrument must be blanked first

GENESYS On-board Software

Live Display Application

Live Display Application

In the live display mode, the instrument performs continuous absorbance measurements
automatically.

42 GENEYS Family Spectrophotometers Thermo Scientific

Fixed Application

Measure
absorbance

Measure
transmittance

Select
equation
template

2

GENESYS On-board Software

Fixed Application

Thermo Scientific GENEYS Family Spectrophotometers 43

2

Selected units will be displayed on the
measurement screen and print out.

At most two wavelengths and two factors
are supported

Factor values are substituted in the equation and ABS values at defined wavelengths
are substituted after measurement

GENESYS On-board Software

Fixed Application

Follow the prompts to complete measurement. When an equation type with two wavelengths is
selected, the instrument will acquire measurements at the two selected wavelengths.

44 GENEYS Family Spectrophotometers Thermo Scientific

Scan Application

Scan range:

Between 190 nm and 1100 nm for UV-Vis

Between 325 nm and 1100 nm for Vis
instruments

Interval:

Specifies how frequently the instrument will
acquire a measurement.

In this illustration, data will be captured every
2nm.

Fast, Medium, Slow scan speeds limit the number of
data interval options offered

2

GENESYS On-board Software

Scan Application

Speed Interval Options

Fast 5nm, 2nm

Medium 5nm, 2nm, 1nm

Slow

a

0.1 nm interval option is available only on GENESYS 180
instruments

5nm, 2nm, 1nm, 0.5nm, 0.2nm,

a

0.1 nm

Thermo Scientific GENEYS Family Spectrophotometers 45

2

To view more sample data
options

to run Peak Pick

Three peaks found in the
region above the ABS cursor
and between wavelength
cursors

Adjust
wavelength
cursors to
narrow
regions of
interest

Adjust ABS cursor to
narrow region of interest

Show
peaks,
valleys or
both

Rename data

All data must be saved for data integrity. If a
custom name is not provided, the software
assigns a default name.

Find the experiment in
the Data Viewer using
this name

GENESYS On-board Software

Scan Application

Analyzing First Sample

46 GENEYS Family Spectrophotometers Thermo Scientific

Quant Application

Add standards and
select curve type

The equation type used
to fit the standard
measurement data is
determined by this
equation

Selects units in which the
sample measurements are
displayed and printed

Select if 1 or 2 replicates
are needed

Save method

Once sufficient unique standard concentration
values are provided, the calibrate button is enabled.

The number of unique standard concentration
values are determined from the equation of the
curve type.

Calibrated and Uncalibrated Quant Methods

When method is saved after a standard curve is calibrated, the method is saved as a calibrated
method. Since the standard curve is already available, it is possible to proceed directly to the
sample measurement screen

2

GENESYS On-board Software

Quant Application

Thermo Scientific GENEYS Family Spectrophotometers 47

2

Equation and r-square
calculated

Measure is enabled after all
standards are measured

Change curve type to find new fit. R-squared
value is re-calculated

after sample measurement is
complete

New sample

Add standards and select curve type

GENESYS On-board Software

Quant Application

48 GENEYS Family Spectrophotometers Thermo Scientific

Cell Changer Setup in Quant

Cell changer with standards

When configuring the cell changer in Quant, the first cell changer will always be configured
with standards. The number of standards can only be configured on the Quant Home page.
The first cell in the standards cell changer will always be blank.

2

GENESYS On-board Software

Quant Application

For details on how to setup a cell-changer, see Cell Changer Configuration.

Thermo Scientific GENEYS Family Spectrophotometers 49

2

GENESYS On-board Software

C-Mode

C-Mode

50 GENEYS Family Spectrophotometers Thermo Scientific

Kinetics Application

Create new method with
settings as shown

1 2

Notice measurements are measured
every “interval” time

Operation of the Kinetics Application is best explained by walking through an example.

2

GENESYS On-board Software

Kinetics Application

Thermo Scientific GENEYS Family Spectrophotometers 51

2

clock to add more time

to update experiment run time

The experiment run time is updated and
chart scaled

Experiment run time may be extended any
number of times before experiment run
time expires

Complete popup is visible after every
measurement is complete

Extend again

Experiment time and chart are updated
again

GENESYS On-board Software

Kinetics Application

52 GENEYS Family Spectrophotometers Thermo Scientific

2

Stops the experiment
immediately

Adjust cursors

After data acquisition is
complete, the Results
page is displayed. The
linear rate of reaction is
calculated.

Slide the cursors to
adjust the region where
rate is calculated.

Export

Select USB port with flash drive

GENESYS On-board Software

Importing Methods

Importing Methods

Thermo Scientific GENEYS Family Spectrophotometers 53

2

Select location

New folder on
USB

New method
file on USB

GENESYS On-board Software

Exporting Data and Methods

Exporting Data and Methods

Performance Verification Tests and Reports

Running Performance Verification Tests

Customized Performance Verification Tests

54 GENEYS Family Spectrophotometers Thermo Scientific

GENESYS instruments are preconfigured with default Performance Verification Tests. These
tests cannot be deleted or edited.

Running a performance verification test is similar to running any other experiment method.
Follow the on-screen instructions.

Some Performance Verification Tests can be customized to meet specific verification
requirements of users. To customize a test, it must first be duplicated.

2

GENESYS On-board Software

Performance Verification Tests and Reports

Often, standard operating procedures require performance verification of instruments
periodically. Test such as Stray Light, Wavelength Accuracy and Photometric Accuracy require
specific sets of filters and standards. The default performance verification tests are designed to
work with specific standards and filters See Table1. However, these tests can be duplicated and
customized to work with standards of choice.

Table 1. Description of each test and if it may be duplicated

Performance Verification Test Description

Wavelength Accuracy Xenon This test verifies the wavelength axis performance of the

spectrophotometer.

Scans and locates known xenon emission peaks and
verifies that they are found accurately to within the
specification for the instrument.

Drift at 500 nm Measures absorbance at 500 nm at 1 minute intervals

for an hour. Measured at 0A (open beam). Blanks and
reports maximum deviation from zero. Compares result
to specification for the instrument.

Result should be less than the specification.

Noise 0A at 500nm

Records 60 ABS measurements at 1 second intervals.
Returns RMS (root mean square) of data set as noise

Noise 1.0A at 500 nm

Noise 2.0A at 500 nm

value and compares specification for the instrument.

Insert 1A or 2A nominal absorbance neutral density
glass filter for those measurements.

Result should be less than the specification.

Can
Duplicate?

No

No

No

Note: Noise in the GENESYS instruments is so low that
the result is reported with more than the usual 3 decimal
digits.

Baseline Flatness 1000 to 200 nm Measures any systematic deviation from perfect zero

No
when scanning across the common wavelength range.
Data is smoothed to remove the impact of noise (noise
can be measured separately). Result is the maximum
deviation from zero and is compared to the specification
for the instrument.

Result should be less than the specification.

Thermo Scientific GENEYS Family Spectrophotometers 55

2

GENESYS On-board Software

Performance Verification Tests and Reports

Performance Verification Test Description

Stray Light SRE 220 filter (UV-Vis models)

Stray Light SRE 400 Filter (Vis models)

Measures stray light at the specified wavelength.

The filter is a long pass filter that cuts on slightly above
the test wavelength. At the test wavelength it should be
entirely dark – i.e. 0%T. Longer wavelengths pass
through the filter, therefore any transmittance measured
at 220 nm is actually photons of longer wavelength that
are “stray light”. Sources of stray light include second
order effects imperfections in the grating, and
imperfections or dirt on mirrors.

The measured transmittance is compared to the
specification for the instrument.

Test measures transmittance at the specified wavelength.

Result should be less than the specification.

Can

Duplicate?

Yes

56 GENEYS Family Spectrophotometers Thermo Scientific

2

GENESYS On-board Software

Performance Verification Tests and Reports

Performance Verification Test Description

Wavelength Accuracy This test verifies the wavelength axis performance of the

spectrophotometer.

Use this test with a calibrated wavelength filter such as
the

filter from the SPECTRONIC Standards 2 Kit or

a holmium or didymium glass filter.

User enters the peak wavelengths and calibration
uncertainty from the calibration certificate.

The instrument scans across the relevant wavelength
range and locates the center of the peak.

Reported wavelength should agree with certificate
wavelength to within the sum of the instrument
specification and the calibration uncertainty.

Photometric Accuracy This test verifies the photometric (absorbance)

performance of the spectrophotometer.

Use this test with one or more calibrated absorbance
filter(s) such as those found in the SPECTRONIC
Standards 2 Kit to test accuracy in the visible region. Use
calibrated potassium dichromate solution cells, metal on
quartz filters, or other recognized calibrated standard

a

materials for the UV region.

Multiple filters calibrated
at the same wavelengths but different absorbance values
can be configured in a single test.

Can
Duplicate?

Yes

Yes

User enters

•calibration wavelengths

• certificate absorbance values

• calibration uncertainty value

from the calibration certificate. User also enters the
performance specification for the absorbance value being
tested from the specification sheet for the instrument.

b

The instrument measures and reports the absorbance at
each specified wavelength. A 1s integration time is used.

Reported absorbance should agree with certificate
absorbance to within the sum of the instrument
specification for that absorbance level and the
calibration uncertainty.

Thermo Scientific GENEYS Family Spectrophotometers 57

2

GENESYS On-board Software

Performance Verification Tests and Reports

a

We recommend against use of didymium glass “dual standard” filters calibrated for both wavelength peaks and photometric accuracy. Customers
have reported difficulty in reproducing calibration values for this type of standard although their instruments do reproduce the calibration values
for other, more widely accepted and recognized standards. Customers who call for support because an instrument fails a photometric accuracy
test with a didymium photometric standard may be required to verify photometric accuracy with a different standard before return shipment for
warranty service is authorized.

b

Future releases of software may include a look-up table that populates the instrument specification based on the user-entered certificate
absorbance value.

Customizing Stray Light Test

58 GENEYS Family Spectrophotometers Thermo Scientific

2

Save

Set up wavelength

Select target
tolerance

Rename

Custom Stray Light performance
verification test is created

GENESYS On-board Software

Performance Verification Tests and Reports

Thermo Scientific GENEYS Family Spectrophotometers 59

2

Toggle to perform wavelength
repeatability test

GENESYS On-board Software

Performance Verification Tests and Reports

Customizing Wavelength Accuracy Test

60 GENEYS Family Spectrophotometers Thermo Scientific

Customizing Photometric Accuracy Test

A typical standards kit for photometric accuracy comes with a Certificate of Calibration. This
section illustrates how to configure a photometric accuracy test for custom standard kits.

2

GENESYS On-board Software

Performance Verification Tests and Reports

Thermo Scientific GENEYS Family Spectrophotometers 61

2

Standard ID may be
used to identify the
corresponding filter
in a filter set

Standard ID may be used to identify the
corresponding filter in a filter set

GENESYS On-board Software

Accessory Setup

Accessory Setup

Cell Changer Configuration

Cell changer setup in the Scan Application is used as an example in this section. However, the
same steps can be followed for all other applications.

Cell changer setup in Quant requires a few extra steps. See Cell Changer Configuration.

62 GENEYS Family Spectrophotometers Thermo Scientific

Sipper Configuration

Fast, Medium, Slow
Set up the speed of the sipper for an
uncalibrated sipper.

These parameter set the time for which the
sipper performs this step.

The parameters are applied only when the
corresponding step is used in a workflow.

See “Custom Workflows” on page 68

2

GENESYS On-board Software

Accessory Setup

Thermo Scientific GENEYS Family Spectrophotometers 63

2

After configuration,

Configured workflow is Sip, Measure and
Rinse

Always blank first

Sipper prompts to
allow setting up the
blank

when
ready

GENESYS On-board Software

Accessory Setup

Measurements

64 GENEYS Family Spectrophotometers Thermo Scientific

2

After sipping, a measurement is made
and the rinse stage begins.

GENESYS On-board Software

Accessory Setup

Message provides information and next
steps of current state

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2

Select a speed,

Enter a target value

After sipper completes sipping, measure the
sipped volume and enter here.

GENESYS On-board Software

Accessory Setup

Calibrated Sipper

By default, the sipper is configured in an uncalibrated mode. Calibrating a sipper allows
precise control over the volume of the liquid drawn.

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2

New estimated sipping time is
calculated from the actual
sipped volume

The sipper may be re-calibrated multiple
times

Enter new measured volume

1

after calibration is satisfactory

Estimated time is
re-calculated

Volume values may be used for setting
stage parameters

Parameters can now be set in volume

A rate is calculated

GENESYS On-board Software

Accessory Setup

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2

1

2

to add new stage

after calibration is satisfactory

GENESYS On-board Software

Accessory Setup

Calibrating the Sipper

The sipper calibration aims to pump 10 mL or a different volume that you specify. We
recommend 10 mL as a good choice for most applications. You will require a method to
determine how much volume has been pumped. We suggest one of two:

• Pump out of a filled 25 mL graduated cylinder. Note the volume on the scale before and

• Pump out of a weighed beaker or flask. Weigh the beaker again after pumping and

Custom Workflows

If an SOP calls for a specific workflow, the sipper configuration offers customization of
workflow.

after pumping. Calculate the volume pumped by subtraction.

calculate the volume pumped by multiplying the difference in mass by the density of the
solvent. The density of water can be taken as 1.00 g/mL for most purposes. However, if
you require particularly high precision in pumping small volumes you may wish to use
purified water and the literature value for density of water at the temperature used.

The workflow will now be saved with the method and will execute with the method.

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Note If the instrument does not detect a sipper, a warning will be displayed. This warning

after setting parameters

Explanation of parameters is provided

Equilibration time in minutes:

anytime to review configuration

parameters

Peltier is setup

will disappear once a sipper is connected.

Peltier Configuration

2

GENESYS On-board Software

Accessory Setup

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2

anytime Peltier status

If configured, Equilibration countdown
begins

GENESYS On-board Software

Accessory Setup

Temperature setpoint:

The instrument starts measurement when the block temperature of the Peltier reaches the
temperature setpoint.

Equilibration time in minutes:

Heat transfer from the block to the sample is not instantaneous; the equilibration time acts as
delay between when the block temperature reaches the setpoint and when the instrument
begins measurement.

Temperature tolerance:

The instrument begins measurement as soon as the Peltier block temperature falls within the
temperature tolerance range about the temperature setpoint.

Stir speed:

This parameter ranges from off to 10 and sets the speed at which the stirrer rotates.

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Accessory Configuration in Scan

to set up

Choose accessory type

(in this case, the 8-cell changer)

Prepare cell changer

Match cell configuration to cell changer configuration

Leave blank Skip

Cells 2 through 7 samples

2

GENESYS On-board Software

Accessory Setup

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2

1

2

3

GENESYS On-board Software

Accessory Setup

Remember, first cell always blank Remember, first cell always blank Perform scan analysis on any sample

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2

Equals number of cell
changers

Always blank

This blank applied to all
subsequent
measurements

Others may be configured
without restrictions

New blanks applied to
subsequent
measurements

after replacing
cell changer

Next cell changer will
start #2 appended

GENESYS On-board Software

Accessory Setup

Measurement will stop after first cell changer.

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2

to view accessory configuration

GENESYS On-board Software

Accessory Setup

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SmartStart

Mark method as
Smart Method

Toggle to unselect.

1

Go to Settings

2

SmartStart™ allows creation of shortcuts to favorite methods on the Home screen.

The Home screen will only show favorite methods.

Configuring and Switching to SmartStart Mode

2

GENESYS On-board Software

SmartStart

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2

3

Instrument in Smart
mode

Only Smart Methods
displayed

1

Go to Settings

2

3

Normal Mode

GENESYS On-board Software

SmartStart

Exiting SmartStart Mode

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Connecting to VISIONlite

when ready

1 2

3

VISIONlite™ is a PC based software developed by Ascanis and provides the ability to control
the GENESYS instruments via a USB cable. VISIONlite needs to be purchased separately and
a quote may be provided <need furl from Manas/Kathy Callaghan>. More information on the
capabilities of the software may be obtained at <need furl from Manas/Kathy Callaghan>.

After installing VISIONlite, connect the special cable provided with the VISIONlite for
GENESYS package between a USB port on the instrument and one on the PC.

Enable the VISIONlite Mode

2

GENESYS On-board Software

Connecting to VISIONlite

When in this mode, the PC will display a notification indicating a GENESYS instrument has
been detected. VISIONlite has now connected to the instrument and can be used to capture
data and perform advanced calculations.

Thermo Scientific GENEYS Family Spectrophotometers 77

2

GENESYS On-board Software

Connecting to VISIONlite

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3

Printer Setup and Wi-Fi Printing

1

2

3

Setting Up USB Printer

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3

5

4

Select USB printer indicated by the USB
symbol

Printer Setup and Wi-Fi Printing

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3

Settings > Network

Select the printer “SSID”

Connect to printer SSID or SSID of the
network to which the printer is connected.

Set up the printer

Select printer

Select printer model

Printer Setup and Wi-Fi Printing

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3

Connect

Preview and print

Preview and print

Printer Setup and Wi-Fi Printing

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Setting Up Thermal Printer

1

2

Confirm the Thermal Printer is installed on the instrument. See Printer for installation
instructions.

3

Printer Setup and Wi-Fi Printing

The print option in all applications will only print to the printer configured under settings.
repeat the steps and re-configure the printer.

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3

to access instrument settings

Configuring Network Paths

Configuring Network Paths

When connected to a Wi-Fi or a wired network connection, the instrument can export
methods and measurement data to a network location. Multiple network paths may be
configured through the Network settings page.

Settings

Setting Description

Smart Start See “SmartStart” on page 25

Languages Selecting a language will set up the locale settings including

Date-Time format and number format. V1.0 of the software does not
support multiple languages for the applications.

Display Adjust brightness and toggle screen saver.“Setting Up USB Printer”

on page 79

Sounds Mute or adjust sound volume.

Network Set up the network path.

Printer Set up printing. See “Printer Setup and Wi-Fi Printing” on page 12

84 GENEYS Family Spectrophotometers Thermo Scientific

Settings

Setting Description

Date and Time

Disk Space Displays the remaining available instrument memory.

Lamp Status Provides the remaining lamp life in percent.

Software Update

Log Export instrument logs to a USB or a network location. Provide these

logs when asked during service or support.

About This screen provides information about the instrument. Provide this

information when requested during service or support.

VISIONlite Enable connection to VISIONlite from this page.

3

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Settings

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4

BioMate 160 Applications

Wavelength fixed at 600 nm

Only Peltier accessory allowed

All applications described here are only available on the BioMate 160 instrument.

OD600

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4

Baseline Correction
allows

BioMate 160 Applications

dsDNA

The reference wavelength setting allows the user to correct for any baseline shift caused by
scatter from particulates suspended in the sample. This is a relatively common phenomenon
in samples that are derived from lyophilized tissue samples. Choose a reference wavelength
close to the measurement wavelength(s) where the absorbance of the sample is expected to
be zero. The instrument will record the actual absorbance at this wavelength and subtract
that value from the “raw” absorbance at the measurement wavelength(s) before performing
calculations with that/those data. 340 nm is a commonly accepted reference wavelength for
DNA measurements.

Example calculation using a reference wavelength: 260/280 ratio

88 GENEYS Family Spectrophotometers Thermo Scientific

RNA

4

BioMate 160 Applications

Protein BCA

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4

BioMate 160 Applications

Protein Bradford

Protein Bradford method is performed at a wavelength of 595 nm. The workflow is similar
to Quant application

90 GENEYS Family Spectrophotometers Thermo Scientific

Protein Lowry

4

BioMate 160 Applications

Protein Lowry method is performed at a wavelength of 650 nm. The workflow is similar to
Quant application

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4

BioMate 160 Applications

Protein Pierce 660

Protein Pierce 660 method is performed at a wavelength of 660 nm. The workflow is similar
to Quant application

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Protein Rapid Gold BCA

Add a new network path

Protein Rapid Gold BCA method is performed at a wavelength of 660 nm. The workflow is
similar to Quant application.

4

BioMate 160 Applications

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4

Custom name to identify path

Full network path in the format shown
Toggle if the path requires authentication

If network location requires authentication, follow the prompts

Save location after all fields are filled

BioMate 160 Applications

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