Thermo Scientific FINNPIPETTE PDP Instructions For Use Manual

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2 5 .0

Labsystems

GRIPPY

HANDLE

DIGITAL
DISPLAY

FINNTIP PDP

OPERATING
BUTTON

FINNPIPETTE PDP

INSTRUCTIONS FOR USE

Thermo Electron Oy
P.O.Box 100, Fin-01621 Vantaa, Finland
Tel. [phone], fax -358-9-3291 0414
www.thermo.com/finnpipette

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PRODUCT DESCRIPTION

The FINNPIPETTE PDP is a continuously adjustable,
positive displacement pipette specially designed for PCR
(Polymerase Chain Reaction) [1] work.
It operates on the positive displacement principle (i.e. no
air interface) and uses detachable, disposable tips with a
pre-assembled plunger.
FINNPIPETTE PDP pipettes cover a volume range of 0.5 µl
to 200 µl.

RAW MATERIALS

The FINNPIPETTE PDP is made of mechanically durable and
chemically resistant materials:

Operating button PA
Handle polyamide
Tip ejector polyamide
Tip cone PVDF

THE DIGITAL DISPLAY (Fig.1)

The adjusted delivery volume is clearly indicated by the
digital display on the pipette handle.
[1] The Polymerase Chain Reaction process PCR, is
covered by US patents issued to the Cetus Corporation.

DESCRIPTION OF FINNTIP PDP (Fig.4)

FINNPIPETTE PDP tips are supplied in racks of 96 or bags
of 960. The product code numbers are as follows:

Cat.No. Volume Qty

9403070 0.5-25µl 960/bag
9403080 20-200µl 960/bag

PIPETTE OPERATION

SETTING THE DELIVERY VOLUME (Fig.2)

A) The delivery volume is set using the operating button

on the top of the pipette. To increase the delivery
volume turn the operating button counterclockwise. To
decrease, turn it clockwise.

B) Make sure that the desired delivery volume clicks into

place and that the digits are completely visible on the
handle display.

C) Do not set volumes outside of the pipette´s specified

volume range!

ATTACHING A TIP (Fig.5)

Attach a Finntip PDP from a rack as follows:

1. Push the pipette firmly onto the tip until the tip and cone
snap.

2. Without removing the tip from the tray press the
operating button to the first stop to engage the plunger.

3. The tip and plunger are now attached for pipetting.

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TIP EJECTION (Fig.3)

1. To remove the tip and plunger from the pipette simply
press the operating button all the way down.

2. Then release the operating button.

3. This action simutaneously ejects the tip and the
plunger.

PIPETTING TECHNIQUE

Figure 6

A = Ready Position
B = Pipetting Stop
C = Tip Ejection

Ensure that clean tips are firmly pushed on to the tip cones
of the pipette.
The operation of the pipette is controlled by the operating
button. For the best possible accuracy:

Operate the button slowly and with steady speed at all
times, particularly when working with liquids having high
viscosity. Never let the operating button snap back. When
working with serum or biological fluids prime the tips with
the liquid before pipetting. This is done by filling and
emptying the tips.

Hold the pipette vertically (10 degrees maximum from
vertical) during the liquid intake. For maximum hand
control, hold the pipette tightly in the hand with the
"Grippy" resting on the index finger.
Fill a clean reagent reservoir with the liquid to be
dispensed.

1. Depress the operating button to the pipetting stop.

2. Dip the tip just under the surface of the liquid in the
reservoir (2-3 mm) and slowly release the operating
button. Withdraw the tip from the liquid touching them
against the edge the reservoir to remove excess liquid.

3. Deliver the liquid by gently depressing the operating
button to the pipetting stop.

4. Release the operating button to the ready position.

If necessary, change the tips and continue with the
pipetting.

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MAINTENANCE

When the FINNPIPETTE PDP is not in use make sure it is
safely stored in a vertical position, preferably on a
Finnpipette Stand.

SHORT TERM CHECKING

The pipette should be checked at the beginning of each
day for dust and dirt on outside surfaces of the pipette.
Particular attention should be paid to the tip cones.
Solvents should not be used for cleaning the pipette.

LONG TERM MAINTENANCE

If the pipette is used daily it should be checked at least
twice a year. The servicing procedure starts with
disassembly of the pipette.

1. To remove tip cone cover:
Insert the tooth of the service tool into the opening at
the base of the tip cone cover.

2. Pull away the tip cone cover

3. Remove the tip cone by turning it counterclockwise
with the service tool.

4. Pull out the piston.

5. Clean the piston and the piston spring with a dry
napless cloth.

6. Grease the cleaned parts with lubricant provided in
the package.

7. Reassemble the parts.

CALIBRATION

All Finnpipettes are factory calibrated and adjusted to
give the volumes as specified with distilled or deionized
water. Normally, the pipettes do not need adjustment,
but they are constructed to permit recalibration and
adjustment for liquids of different temperature and
viscosity.

DEVICE REQUIREMENTS AND TEST CONDITIONS

An analytical balance must be used. The scale graduation
value of the balance should be chosen according to the
selected test volume of the pipette:

Volume range readable graduation

under 10µl 0.001mg
10-100µl 0.01mg
above 100µl 0.1mg