Thermo Scientific Finnpipette Novus Instructions For Use Manual

Thermo Fisher Scientific
Thermo Scientific Novus SCP/MCP User Manual 1
Finnpipette® Novus
Single Channel & Multichannel
User Manual
2 Thermo Scientifi c Novus SCP/MCP User Manual
Thermo Fisher Scientifi c
CONTENT
PRODUCT DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Raw materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Description of tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
PACKAGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
GETTING STARTED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Power Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Power plug adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
Installing the battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Charging the battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Adjusting the trigger position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Tip ejection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Shelf hanger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
PIPETTE OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Choosing pipetting functions and speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
The Menu Map . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Pipette (Forward technique) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Rpipet (Reverse & Repetitive) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Reverse technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Repetitive technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Stepper (multi dispensing) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Dilute . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Additional functions in program mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Mix + Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Pipette + Count . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Seq stepper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Mix + Dilute . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Seq + Aspirate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Calibrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Name. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Buzzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Backlight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
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CALIBRATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Device requirements and test conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Calibration Counter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Checking the calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Two point calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
One point calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Formulas for calculating results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Conversion of mass to volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Inaccuracy (systematic error) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Imprecision (random error) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Daily checking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Short-term Service Single Channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Long-term service Single Channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
Short-term Service Multichannel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Long-term service Multichannel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Service Instructions for Multichannel Pipette Tip Cones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Replacing the battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Opening the Version 1 battery lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Opening the Version 2 battery lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Sterilization Single Channel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Sterilization Multichannel pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
TROUBLE SHOOTING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
TECHNICAL DATA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
SPARE PARTS SCP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31-33
SPARE PARTS MCP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34-37
WEEE Compliance:
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Scientifi c has contracted with one or more recycling/disposal companies in each EU Member State, and this product should be disposed of or recycled through them. Further information on Thermo Scientifi c’s compliance with these Directives, the recyclers in your country, and information on Thermo Scientifi c products which may assist the detection of substances subject to the RoHS Directive are available at
www.thermoscientifi c.com/WEEERoHS
Product specifi cations are subject to change without prior notice. Finnpipette® and Finntip® are registered trademarks of Thermo Scientifi c. See the latest version at www.thermofi sher.com
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PRODUCT DESCRIPTION
The different models of Finnpipette Novus pipettes cover a volume range from 1 μl to 10 ml.
The different models of Finnpipette Novus pipettes cover a volume range from 1 μl to 1200 μl.
The Finnpipette Novus is an electronically assisted pipette for a wide range of liquid handling operations. Thanks to the electronic motor and electronic control, pipetting is easy and comfortable, yet still fast and accurate. It operates on the air displacement principle (i.e. an air interface) and uses detachable, disposable tips, which are easy to remove with a soft touch tip ejector. The adjustable, index fi nger operated pipetting trigger uses natural hand movement, increasing comfort and reducing the risk of repetitive stress injuries. The Finnpipette Novus provides many functions which are very practical for daily use in laboratory work, such as forward-, reverse- and repetitive pipetting, stepper and diluting modes. The guiding and easy user-interface is very fast to learn. The adjusted delivery volume is clearly indicated in the LCD display on top of the handle. The long lasting Lithium-Ion -battery is always charged with rapid charge technique. If needed, the battery can be charged over the lunch hour.
Order No. Channel Volume Range Finntip
46300000 8 1 µl to 10 µl Flex 10, 10, 20, 50
46300100 12 1 µl to 10 µl Flex 10, 10, 20, 50
46300200 8 5 µl to 50 µl Flex 200, 250 Univ., 200 Ext
46300300 12 5 µl to 50 µl Flex 200, 250 Univ., 200 Ext
46300400 8 30 µl to 300 µl Flex 300, 300
46300500 12 30 µl to 300 µl Flex 300, 300
46300700 16 5 µl to 50 µl 50
46300800 8 100 µl to 1200 µl Flex 1200
Order No. Volume Range Finntip
46200000 1 µl to 10 µl Flex 10, 10, 20, 50
46200100 1 µl to 10 µl Flex 200, 250 Univ., 200 Ext, 300
46200200 5 µl to 50 µl 50
46200300 5 µl to 50 µl Flex 200, 250 Univ., 200 Ext, 300
46200400 10 µl to 100 µl Flex 200, 250 Univ., 200 Ext, 300
46200500 30 µl to 300 µl Flex 300, 300
46200600 100 µl to 1000 µl Flex 1000, 1000, 1000 Ext
46200700 0,5 ml to 5 ml 5 ml
46200800 1 ml to 10 ml 10 ml, Flex 10 ml Ext
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Thermo Scientific Novus SCP/MCP User Manual 5
The Finnpipette Novus is made of mechanically durable and chemically resistant materials. The tip cone modules can be repeatedly autoclaved at 121°C.
Note The 1200 µl module is NOT autoclavable.
Finntips are recommended for use with the Finnpipette Novus. They are made of natural colour polypropylene, generally regarded as the only contamination free material suitable for tips. Finntips are also autoclavable (121°C).
Package
The complete Finnpipette Novus package contains:
1. Finnpipette Novus
2. Charger
3. Shelf hanger (Order No. 2206040)
4. Piston removal tool
5. Instructions for use
6. Bag of grease 1g (Order No. 3300200)
7. Sample Finntips
getting started
Remove the content from the package and verify that all items listed above are included. Inspect for possible shipping damage. Make sure that the pipette is desired volume range and that the voltage of the charger is correct.
Note If the device is not used according to manufacturers instructions the protection provided by the device cannot be guaranteed.
Power Requirements
- INPUT: 100-240V~50/60Hz, 150mA
- OUTPUT: 5,0V 0,5 A max
Power plug adapters:
1. Australia
2. Europe
3. United Kingdom
4. USA / Japan
5. Power supply unit
Insert the country specific power plug adapter into the power supply unit.
Note The charger’s plug must be inserted in a socket that is easy to reach and from which it can be easily unplugged in case of emergency.
Raw materials
Description of tips
Power supply
3
1
4
2
5
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Thermo Fisher Scientific
Warning Use of incorrect power supply units may result in fatal injuries and damage to the device.
Use of incorrect power supply units may results in overheating, burning, melting, short-circuiting of the Thermo Scientific Finnpipette Novus pipette or similar damage. Only use the supplied power supply unit for charging the pipette. You can recognize the correct power supply by the Thermo Scientific logo and the pipette name on the power supply unit. Do not charge the pipette in a hot location (>40°C)
Note If the pipette is supplied with the battery attached the below installation is not needed.
- Check, that the battery wires are as shown in the picture.
Note Do not remove the tape that is holding the wires
- Slide in the battery (label side visible)
- Connect the battery connector.
- Ensure that the wires are flat, not twisted
- Attach the battery lid as shown in the picture
Installing the
battery
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Warning Use only the original Finnpipette Novus charger and battery pack.
The pipette battery may be empty when delivered and must be charged before initial use. Connect the lead of charger to the socket on the back of pipette. Then connect the charger to an AC wall socket. If the battery is completely empty, it may take a few minutes before the pipette will turn on. You can use the pipette while the charger is connected. The charging time is typically less than one hour. An indicator in the LCD display shows the charge level of the battery.
When the indicator shows empty battery, pipetting is no more possible, and the pipette has to be charged again. To extend battery life cycle, it’s recommended to charge the pipette every two months even if/ even though the pipette is not used daily. A typical Finnpipette Novus battery lasts for around 500 charging cycles. Please note that an old battery can cause the pipette to malfunction. We recommend that you change the battery every 3 years.
The index fi nger operated trigger, which activates the piston movement, can be adjusted by rotating it 60 degrees to both directions of the center position. Usually right handed operators turn it left (counter clockwise) to get the best possible position for the thumb to eject the tip. See picture on page 9.
To help eliminate the risk of contamination, each pipette is fi tted with a tip ejector system. The tip ejector system consists of a soft-touch tip ejector and specially designed gearing mechanism. To release the tips, point the pipette at suitable waste receptacle and press the tip ejector with your thumb.
You can attach the pipette shelf hanger on a counter, pipette stand or anywhere where you want to hang your pipette. Clean the area where you plan to attach the shelf hanger. Apply two stickers to the underside of the shelf hanger. Press the shelf hanger fi rmly into place — on a shelf, countertop or pipette stand. To use, hang the grippy fi nger rest on the shelf hanger.
Charging the
battery
Adjusting the
trigger position
Tip ejection
Shelf hanger
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Safety Precautions
Warning Novus pipette contains a Li-Ion battery and a potential malfunction
of the battery (short circuit, mechanical damage, overheating etc) may cause explosion.
Warning Incorrect handling of the battery or pipette may cause personal injury. Do not drop the battery. Do not expose battery to any kind of mechanical stress or extreme temperatures: above 60 °C (45 °C when charging) or under 0 C°.
Warning Do not immerse the unit in liquid and do not clean the upper part of the pipette by spraying. Fluid seepage can damage internal components and may cause a safety risk.
Warning Follow the Novus pipette instructions presented in the user manual in part , Technical Data. If the instructions are not followed it may cause a safety risk.
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PIPETTE OPERATION
To choose the pipetting function press Menu (left selection key). Scroll the function list and select the desired function with OK (right selection key). In most cases the volume can be selected simply by pressing the scroll key up or down. Accept the volume with OK. In some cases the initial piston position has to be changed and a text PRESS TRIGGER appears on display. Press the trigger to move the piston to the new initial position.
Pipetting speeds can be selected with the right selection key whenever the text SPEED is displayed. Press SPEED and the speed in starts to blink. Select the speed with scroll key and accept with OK. Now the speed out starts to blink. Select the speed with scroll key and accept with OK.
Choosing pipetting
functions and
speed
Function
Left Selection Key
Volume indicator
Right Selection Key
Scroll Key
Tip Ejector Lever
Battery indicator
Speed indicator
Speed in / out
Trigger and fi nger rest adjustable
Trigger
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Choose the PIPETTE function as described above.
Select the pipetting volume simply by pressing the scroll key up or down.
Accept the volume with OK. Optionally press SPEED and the speed in starts to blink. Select the speed with scroll key and accept with OK. Select the speed out with scroll key and accept with OK.
1. Dip the tip under the surface of the liquid in the reservoir and press the trigger. The liquid is drawn in to the tip.
2. Wait until the liquid is not moving in the tip and withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid.
3. To deliver the liquid press the trigger and hold it down. The blow-out is also included to empty the tip.
4. Release the trigger to return to the ready position.
If necessary, change the tip and continue pipetting.
The Menu Map
Pipette (Forward
technique)
PIPETTE RPIPET STEPPER DILUTE PROGRAM OPTIONS
PIPETTE MIX+PIPETTE PIPETTE+COUNT RPIPET STEPPER SEQ+STEPPER DILUTE MIX+DILUTE MANUAL SEQ+ASPIRATE
CALIBRATE SERVICE LANGUAGE NAME POWER BUZZER VERSION BACKLIGHT
PROG 1 PROG 2
. . .
PROG 9
TWO POINTS ONE POINT COUNTER
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With RPIPET function both reverse and repetitive techniques are possible. Choose the RPIPET function as described above.
Select the pipetting volume simply by pressing the scroll key up or down. Accept the volume with OK. Press SPEED and the speed in starts to blink. Select the speed with scroll key and accept with OK. Select the speed out with scroll key and accept with OK.
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency to foam easily. The technique is also recommended for dispensing very small volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Dip the tip under the surface of the liquid in the reservoir and press the trigger. This action will fi ll the tip.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by shortly pressing the trigger. Some liquid will remain in the tip and this should not be included in the delivery. A text BLOWOUT is displayed.
4. To empty the tip, press trigger again.
If necessary, change the tip and continue pipetting.
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Dip the tip under the surface of the liquid in the reservoir and press the trigger. This action will fi ll the tip.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by pressing and holding the trigger down. Some liquid will remain in the tip and this should not be included in the delivery.
4. Dip the tip again to reagent reservoir and release the trigger. This action will fi ll the tip again.
5. Continue pipetting by repeating steps 3 and 4.
6. To empty the tip completely, dispense by shortly pressing the trigger. Some liquid will remain in the tip and this should not be included in the delivery. A text BLOWOUT is displayed.
7. To empty the tip completely, press trigger again.
If necessary, change the tip and continue pipetting.
Rpipet (Reverse &
Repetitive)
Reverse technique
Repetitive
technique
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With STEPPER function repeated dispensing of selected volume is possible. Choose the STEPPER function as described above.
Select the pipetting volume simply by pressing the scroll key up or down. The display shows always the maximum number of steps during volume selection. Accept the volume with OK. Next choose the number of steps with scroll key and accept with OK.
Optionally select the speed. Press SPEED and the speed in starts to blink. Select the speed with scroll key and accept with OK. Select the speed out with scroll key and accept with OK.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Dip the tip under the surface of the liquid in the reservoir and press the trigger. This action will fi ll the tip.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by pressing the trigger. The display shows the number of steps left.
4. Continue dispensing by repeating step 3. After last step a text BLOWOUT is displayed.
5. To empty the tip completely, press trigger and hold it down.
6. Release the trigger.
If necessary, change the tip and continue pipetting.
Note The pipette can be emptied at any time by pressing CANCEL (left selection key)
With DILUTE function dispensing of two selected volume is possible. Choose the DILUTE function as described above. First volume (VOL 1) is shown on display.
Select the pipetting fi rst volume simply by pressing the scroll key up or down. Accept the volume with OK. Next select the second volume (VOL 2) with scroll key and accept with OK.
Optionally select the speed. Press SPEED and the speed in starts to blink. Select the speed with scroll key and accept with OK. Select the speed out with scroll key and accept with OK.
Stepper (multi
dispensing)
Dilute
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