Thermo Scientific EVOS M5000 User Manual
EVOS™ M5000 Imaging System
For Fluorescence and Transmitted Light Applications
Catalog Number AMF5000
Publication Number MAN0017563
Revision A.0
For Research Use Only. Not for use in diagnostic procedures.
Revision
Date
Description
Information in this document is subject to change without notice.
Revision history MAN0017563
A.0 07 Aug 2018 New user guide for the EVOS™ M5000 Imaging System
Important Licensing Information
These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and
conditions of all applicable Limited Use Label Licenses.
Manufacturer: Life Technologies Corporation | 22025 20TH Ave SE | Bothell, WA 98021.
Trademarks
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
Cy is a registered trademark of GE Healthcare UK Limited. Windows is a registered trademark of Microsoft Corporation. Kimwipes is a
registered trademark of Kimberly-Clark Corporation. Hoechst is a registered trademark of Hoechst GmbH.
© 2018 Thermo Fisher Scientific Inc. All rights reserved.
Contents
About this guide ...................................................................................................................... 3
1. Product information .......................................................................................................... 4
Product description ......................................................................................................................................... 4
Standard items included ................................................................................................................................. 5
Instrument exterior components and mechanical controls ....................................................................... 6
Graphical user interface (GUI)....................................................................................................................... 8
2. Installation ........................................................................................................................ 9
Operating environment and site requirements ........................................................................................... 9
Prepare for installation ................................................................................................................................. 10
Install the instrument .................................................................................................................................... 10
3. Capture images ................................................................................................................ 15
Overview ........................................................................................................................................................ 15
Capture images .............................................................................................................................................. 16
Analyze and annotate captured images ..................................................................................................... 23
Show cell count .............................................................................................................................................. 27
4. Save captured images ...................................................................................................... 34
Save ................................................................................................................................................................. 34
Quick Save images ........................................................................................................................................ 35
5. Capture and save time lapse images ................................................................................ 36
Time Lapse tool.............................................................................................................................................. 36
Run a time lapse routine ............................................................................................................................... 36
6. Capture Z-Stack ............................................................................................................... 43
Z-Stack tool ..................................................................................................................................................... 43
Capture Z-stack images ................................................................................................................................ 43
7. Review saved images ....................................................................................................... 47
Overview ........................................................................................................................................................ 47
Review images ............................................................................................................................................... 47
Analyze saved images .................................................................................................................................. 49
8. Adjust instrument settings ............................................................................................... 50
Overview ........................................................................................................................................................ 50
Adjust objective settings ............................................................................................................................... 51
Calibrate white balance ................................................................................................................................ 53
Set saturated pixel options ........................................................................................................................... 54
General settings ............................................................................................................................................. 55
Configure network settings .......................................................................................................................... 56
EVOS™ M5000 User Guide
1
9. Instrument care and maintenance ................................................................................... 58
General care .................................................................................................................................................... 58
Objective lens care ......................................................................................................................................... 58
Stage care ........................................................................................................................................................ 59
Decontamination procedures ....................................................................................................................... 59
Change EVOS™ LED light cubes ................................................................................................................. 60
Change the objectives ................................................................................................................................... 61
Calibrate the objectives ................................................................................................................................. 62
Appendix A: Troubleshooting ................................................................................................. 64
Image quality issues ...................................................................................................................................... 64
Software interface issues .............................................................................................................................. 65
Mechanical issues .......................................................................................................................................... 65
Appendix B: Graphical user interface (GUI) ............................................................................ 66
Capture tab ..................................................................................................................................................... 66
Review tab ...................................................................................................................................................... 71
Settings ............................................................................................................................................................ 72
Appendix C: System overview ................................................................................................ 74
Technical specifications ................................................................................................................................ 74
Operation principles and technical overview............................................................................................ 75
Appendix D: Safety ................................................................................................................. 76
Safety conventions used in this document ................................................................................................. 76
Symbols on instruments ............................................................................................................................... 77
Safety labels on instruments ........................................................................................................................ 79
General instrument safety ............................................................................................................................ 80
Chemical safety .............................................................................................................................................. 81
Chemical waste safety................................................................................................................................... 82
Electrical safety .............................................................................................................................................. 83
Physical hazard safety .................................................................................................................................. 84
Biological hazard safety ................................................................................................................................ 84
Safety and electromagnetic compatibility (EMC) standards ................................................................... 85
Documentation and support ................................................................................................... 86
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EVOS™ M5000 User Guide
About this guide
Audience
This user guide is for laboratory staff operating, maintaining, and analyzing data
using the Invitrogen™ EVOS™ M5000 Imaging System.
User attention
words
Two user attention words appear in this document. Each word implies a particular
Safety alert words
Three safety alert words appear in this document at points where you need to be
implies a particular level of observation or action, as defined below:
level of observation or action as described below.
Note: Provides information that may be of interest or help but is not critical to the
use of the product.
IMPORTANT! Provides information that is necessary for proper instrument
operation, accurate installation, or safe use of a chemical.
aware of relevant hazards. Each alert word—CAUTION, WARNING, DANGER—
CAUTION! – Indicates a potentially hazardous situation that, if not avoided,
may result in minor or moderate injury. It may also be used to alert against
unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
EVOS™ M5000 User Guide
3
EVOS™ M5000
The Invitrogen™ EVOS™ M5000 Imaging System (Cat. No. AMF5000) is a fully
easy control.
EVOS™ M5000
The EVOS™ M5000 Imaging System is controlled by the integrated Invitrogen™
connectivity to the network and Thermo Fisher Cloud.
Product use
For Research Use Only. Not for use in diagnostic procedures.
1. Product information
Product description
Imaging System
Software
integrated, digital, inverted imaging system for four-color fluorescence and
transmitted-light applications.
It combines precision optics, a five-objective turret, an 18.5 inch high-resolution
LCD display (1920 × 1080 pixel resolution), and a highly sensitive Sony IMX265
monochrome CMOS camera (2048 × 1536 pixel resolution, 3.2 Megapixels) to
acquire images seamlessly through the intuitive user interface using a mouse for
EVOS™ M5000 Software through a graphical user interface (GUI), which is accessed
by the computer mouse and keyboard. The software is pre-installed to the
instrument and starts automatically when the instrument is powered on.
Key features of the EVOS™ M5000 Software include:
• Capture: Allows control over every aspect of the system for image capture
through a simple user interface. All images acquired can be saved in BMP, JPG,
PNG, and TIFF formats, or compiled into a video sequence in AVI or WMV
formats.
• Autofocus: Allows autofocus in fluorescence and brightfield modes.
• Z-stacking: Captures a series of images along the z-axis that can be saved
individually or combined into a Z-stack projection with a greater depth of field
than any of the individual source images.
• Time lapse: Allows you to create a time lapse movie using captured images.
• Review: Allows you to review, measure, and annotate captured images.
• Cell count: Allows you to count cells in fluorescence mode post-acquisition.
• Network and Thermo Fisher Cloud connectivity: Allows Wi-Fi and Ethernet
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EVOS™ M5000 User Guide
EVOS™ M5000
•
- Objectives, as ordered
EVOS™ M5000
•
- Mouse pad
EVOS™ M5000 user
•
- EVOS™ M5000 Imaging System Installation Guide (Pub. No. MAN0017783)
Standard items included
EVOS™ M5000 Imaging System, includes the following components and pre-
Imaging System
Accessories Kit
installed accessories:
- 18.5 in articulated LCD monitor (1920 ×1080 pixel resolution)
- Embedded PC (4 GB RAM)
- Light cube shipping restraint (remove before use)
- Stage lock pin (remove before use)
- EVOS
- Light cube tool (remove before use)
- Blank light cube (remove before use)
- LED light cubes, as ordered
EVOS™ M5000 Accessories Kit (located in the instrument box), contains:
- Wireless mouse and keyboard
- USB receiver (for wireless mouse and keyboard connection)
- USB Wi-Fi adaptor (for wireless network connection to Thermo Fisher
Cloud applications)
- USB 3.0 flash drive, 16 GB (for image storage, and preloaded with user
documentation)
- EVOS
- EVOS
- EVOS
- EVOS
- UV shield assembly
- Condenser Slider, Block (Cat. No. AMEP4688)
- Condenser Slider, 4X Pupil (Cat. No. AMEP4738)
- Condenser Slider, Diffusion for Brightfield Applications (Cat. No.
AMEPDFS1)
- Universal power supply (12 V, 5 A) and power cord (type B, North America)
- EVOS
- Accessories box with adjustable compartments
- Hex driver, 2 mm
™
Condenser Light Shield, 110 mm
™
Vessel Holder, Two 25 × 75 mm slides (Cat. No. AMEPVH001)
™
Vessel Holder, Universal (Cat. No. AMEPVH009)
™
Calibration Slide (Cat. No. AMEP4720)
™
Light Shield Box
™
Dust Cover
EVOS™ M5000 Quick Start Guide, printed (Pub. No. MAN0017765)
documentation
EVOS™ M5000 User Guide
• EVOS™ M5000 Imaging System Installation Guide, printed (Pub. No. MAN0017783)
• Pre-loaded to USB 3.0 flash drive (located in the accessories box):
™
- EVOS
- EVOS
M5000 User Guide (Pub. No. MAN0017763)
™
M5000 Quick Start Guide (Pub. No. MAN0017765)
5
Front view
Stage positioning
Instrument exterior components and mechanical controls
knobs and brakes
LCD monitor Focusing knobs
Condenser slider slot Objective selection wheel
Condenser head Vessel holder
Mechanical X-Y stage Objective
Stage X-axis positioning knob Phase annuli selector
Stage Y-axis positioning knob
Mechanical X-Y stage
Stage X-axis positioning knob
Stage brake for X-axis
Stage Y-axis positioning knob
Stage brake for Y-axis
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EVOS™ M5000 User Guide
Rear view
LCD monitor Network port
Mechanical X-Y stage USB-A 2.0 ports (4×)
Focusing knobs USB 3.0 port (1×)
Power switch Stage X-axis positioning knob
4-pin power input port (12 VDC, 5 A) Stage Y-axis positioning knob*
Display port (video output) * Not visible in this perspective.
EVOS™ M5000 User Guide
7
GUI layout
The GUI of the system consists of the Viewing area on the left and Capture and
and button opens the controls necessary to execute the selected function.
Graphical user interface (GUI)
Review tabs and the Settings and Virtual keyboard buttons on the right. Each tab
Viewing area: Displays the sample.
Capture tab: Contains the controls for image capture.
Review tab: Allows you to review and annotate captured images.
Keyboard button: Opens the virtual keyboard.
Settings button: Opens the Settings tabs, which allow you to select and adjust basic
and advanced system settings.
Note: For more information and detailed descriptions of GUI controls, see
“Appendix B: Graphical user interface (GUI)”, page 66.
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EVOS™ M5000 User Guide
2. Installation
• The dimensions of the EVOS™ M5000 Imaging System are 18 × 23 × 18 in
vibrations from other pieces of equipment. Tabletop centrifuges, vortex mixers,
• Electrical input: 12 VDC, 5 A
Hood setup
Operating environment and site requirements
(W×H×D) (46 × 59 × 46 cm). The system requires a benchtop of approximately
36 × 36 in (92 ×92 cm).
• If the system includes the optional EVOS™ Onstage Incubator (Cat. No.
AMC1000), then add 16 in (40 cm) to the width of the bench.
• Allow at least 5 cm (2 in) free space at the back of the instrument to allow for
proper ventilation and prevent overheating of electronic components.
• Place the EVOS™ M5000 Imaging System on a level surface away from
and other laboratory equipment can vibrate the instrument during a run and
cause a decrease in instrument performance.
• The EVOS™ M5000 Imaging System should be installed away from direct light
sources such as windows. Ambient light can enter the imaging path and affect
the image quality.
• Operating temperature range: 4°–32°C (40°–90°F).
• Relative humidity range: 0–90%.
• Operating power: 100–240 VAC, 1.8 A
• Frequency: 50–60 Hz
IMPORTANT! Do not position the instrument so that it is difficult to turn off the
main power switch located on the back of the instrument base (see page 7). In case
of an instrument malfunction, turn the main power switch to the OFF position and
disconnect the instrument from the wall outlet.
The EVOS™ M5000 Imaging System fits in cell culture hoods that are at least 24 in
(61 cm) deep and 36 in (92 cm) high with a 30 in (76 cm) opening. If your cell
culture hood is smaller, it may be possible to fit the instrument by turning it at a
slight angle.
EVOS™ M5000 User Guide
9
Receive and inspect
1. Verify that the items shown on the shipping list are the same items that you
mishandling on the shipping documents.
Move the
installation site
1. Clear the installation site of all unnecessary materials.
Unpack the
1. Open the shipping box and remove the accessory box.
list of standard items included in the shipment.
Prepare for installation
the shipment
instrument to the
ordered at the time of purchase.
2. Carefully inspect the shipping containers and report any damage to the
Thermo Fisher Scientific service representative. Record any damage or
2. If possible, move the crated instrument and other shipping containers to the
installation site.
CAUTION! PHYSICAL INJURY HAZARD. Lift or move the instrument using
proper lifting techniques. We recommend that you lift or move the crated
instrument with the assistance of others and the use of appropriate moving
equipment. Improper lifting can cause painful and permanent back injury.
Depending on the weight, moving or lifting an instrument may require
two or more persons.
Install the instrument
instrument
2. Carefully lift the instrument out of the box by grasping it firmly with both
hands under the support arm.
3. Place the instrument on a flat, level surface that will be free from vibration and
leave enough room around it for the stage to move freely.
4. Tilt the LCD monitor upright.
5. Examine the instrument carefully for damage incurred during transit.
6. Unpack the accessories box and verify all parts are present. See page 5
for the
IMPORTANT! Do not subject the EVOS™ M5000 Imaging System to sudden impact
or excessive vibration. Handle the instrument with care to prevent damage.
Note: Contact your distributor if anything is missing. If you do not have your
distributor information, contact Technical Support (page 86). Damage claims must
be filed with the carrier; the warranty does not cover in-transit damage.
Note: Make sure to set aside packaging and foam for future transport and storage.
Re-install the stage lock pin and the light cube shipping restraint before moving
or transporting the instrument. Always ensure that the instrument is properly
cushioned and braced to prevent damage.
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EVOS™ M5000 User Guide
Remove shipping
The EVOS™ M5000 Imaging System is equipped with two shipping restraints
before you power on the EVOS™ M5000 Imaging System.
1. Pull firmly to remove the stage lock pin and release the X- and Y-axis brakes.
back of the stage.
Before stage reposition
After stage reposition
3. Unscrew and remove the light cube tool, which secures the shipping restraint
light cube turret.
restraints
(stage lock pin and light cube shipping restraint) to protect the instrument from
shock and vibration during transport. You must remove the shipping restraints
Stage lock pin
Light cube shipping
restraint
Light cube tool
2. Using the X-axis and Y-axis stage positioning knobs, move the stage back to
obtain access to the light cube shipping restraint, which is centered under the
Note: The light cube shipping restraint is secured with the light cube tool to
the blank light cube installed in the light cube turret. Used together, they
immobilize the light cube turret to protect it during transport.
block to the blank light cube.
4. Remove the shipping restraint block and store it in the accessories box.
Removal of the restraint block provides access to the blank light cube in the
Note: The blank light cube is red and does not have the grooved copper top
of the LED light cubes.
EVOS™ M5000 User Guide
11
5. Using the light cube tool, loosen the two screws that secure the blank light cube
7. Place the desired EVOS™ Vessel Holder on the stage.
Install the UV light
1. Verify that the EVOS™ Condenser Light Shield is installed on the condenser
Blank light cube
Thread hold
Screws
to the instrument. You do not need to remove the screws.
6. Screw the light cube tool to the thread hold on the light cube, then lift the blank
light cube up and out of the light cube turret. Store the blank light cube and the
light cube tool in the accessories box.
Note: Store the shipping restraints and the light cube tool for future use in the
accessories box provided with your system. Always re-secure the X-Y stage with
the stage lock pin and re-install the light cube restraint before moving the
instrument.
IMPORTANT! Before changing light channels, ALWAYS verify the light cube
restraint has been removed. Attempting to change the light channels while the
restraint is in place can seriously damage the mechanism. This type of damage is
not covered by manufacturer’s warranty.
shield
assembly. The condenser shield is pre-installed and helps reduce the potential
effects of overhead lighting on your image.
2. Pull the condenser light shield out from the condenser head.
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EVOS™ M5000 User Guide
3. Secure the UV light shield mount to the top of the condenser light shield using
the two screws supplied with the UV shield assembly (not the protruding
Connect the
1. Confirm that the power switch is OFF (located on the back; see page 7).
supply.
screws on the mount).
4. Clip the condenser light shield with the attached UV light shield mount back
onto the condenser head.
5. Peel the protective paper from the UV light shield, then slide the orange UV
light shield over the two protruding screws on light shield mount to attach it
to the instrument.
Note: The UV light shield is provided as a safety feature and should be installed
whenever the unit is in operation. The UV light shield is removable for access to
the condenser sliders used in transmitted light mode. Simply unhook it from the
screws on the UV light shield mount.
instrument
2. Connect the USB receiver for the wireless mouse and keyboard to an
available USB-A 2.0 port (page 7).
3. Connect the power adaptor and power cord. Ensure a tight connection
4. Plug the power adaptor into the power input port on the instrument (page 7).
5. Plug the power cord into a power outlet and check for the light on the power
EVOS™ M5000 User Guide
13
Power ON the
1. Turn the instrument power switch (located on the back; see page 7) to the ON
Connect to a Wi-Fi
network
For instructions on how to connect to a Wi-Fi network and how to map a network
EVOS™ M5000
Imaging System
position.
2. When the Capture tab is displayed, the EVOS™ M5000 Imaging System is
ready to use.
IMPORTANT! All shipping restraints must be removed before turning on the
™
EVOS
M5000 Imaging System to prevent damage (page 11).
drive to save your images, see “Configure network settings”, page 56.
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EVOS™ M5000 User Guide
3. Capture images
Capture tab
The basic functions of the EVOS™ M5000 Imaging System, such as viewing the
Workflow
Select objective and light source
Adjust brightness
Focus on the sample
Find the region of interest
Capture image in a single channel
Analyze and annotate captured images
Show cell count
Save
Overview
sample, setting optimal focus, and capturing and saving images are performed in
the Capture tab, which is the first screen after start-up.
EVOS™ M5000 User Guide
15
Select objective and
1. Place the vessel containing your sample on the stage using the appropriate
2. Set the magnification using the objective selection wheel (page 6).
Capture images
light source
vessel holder.
Note: When capturing images in fluorescence channels, place the light shield
box on the stage, over the sample. This is important for optimal image quality.
3. Select the desired Phase option by turning the phase annuli selector (page 6).
Available options are:
• Oly 4×: Used for low magnification objectives (Olympus
• 4×/10×: Used for medium magnification objectives (EVOS
• 20×/40×: Used for high magnification objectives (EVOS
• Brightfield (phase contrast off)
The active objective and phase ring information is displayed above the
Channel buttons.
4. Select a Channel to turn on the excitation light and enter the instrument in the
Live mode. In this example, the DAPI channel has been selected.
In the Live mode, you can adjust the brightness and configure display settings
for the selected channel, and set the focus on the sample.
™
4× PH)
™
4×/10× PH)
™
20×/40× PH)
Note: You can select only one light source at a time for Live mode to adjust
brightness and set focus. However, you can display and capture multiple
channels simultaneously.
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EVOS™ M5000 User Guide
Adjust brightness
1. For Brightness mode, select Simple or Actual.
- Simple mode allows you to control Brightness as a single
•
parameter.
- Actual mode allows you to adjust Light (i.e., LED intensity),
Exposure, and Gain parameters individually.
2. Adjust brightness using the Brightness controls:
• In the Simple mode, move the Light slider to adjust brightness.
Alternatively, enter the desired brightness value (0%−100%) directly in the
brightness field.
In the Actual mode, move the Light (LED intensity), Exposure, and Gain
sliders to adjust the brightness parameters individually.
Alternatively, enter the desired value for each parameter (0%−100% for
Light, 0−4.00 seconds for Exposure, or 1.0−8.0 for Gain) directly in the
corresponding field.
Note: Optimize the brightness parameters as follows:
• When searching for sample: Increase Gain for a brighter signal and
decrease Exposure for faster frame rate during navigation around the
vessel.
• When capturing image: Decrease Gain to reduce background noise and
increase Exposure to regain signal intensity, as needed.
• For brighter signal: Increase Light intensity for brighter illumination. If
needed, follow by increasing Gain.
• For time lapse imaging: Increase Gain and Exposure, and decrease
Light intensity to reduce photobleaching and phototoxicity.
For example, for overnight time lapse experiments, capture one image
every 30 minutes or less, limit the use of autofocus, and use a channel
other than DAPI for autofocus.
• In the Settings Visuals tab, select Highlight Saturated Pixel to display
the overexposed pixels in the color of your choice. To obtain the
maximum level of brightness without any overexposed areas, dim the
illumination until the highlights disappear.
EVOS™ M5000 User Guide
17
Focus on the
1. Use the Coarse and Fine focus sliders to focus on the sample.
the Coarse focus slider) to set the upper and lower bounds.
3. Repeat the focus procedure for each channel you want to capture.
• When unlocked, only the current channel is affected.
Optional
: Configure
1. Hover the pointer over the Viewing area to reveal the buttons for Display
5. Click the Image Display Settings button again to collapse the controls.
sample
Alternatively, click Coarse and Fine autofocus to autofocus on the sample.
2. To limit the autofocus algorithm to a specific region along the Z-axis, use the
Autofocus Maximum and Autofocus Minimum controls (blue triangles on
Note: You can also focus on the sample manually using the focusing knobs
on the instrument (page 6).
Click Enable focus knob, then turn the focusing
knob until the region of interest is in focus.
4. If you want to preserve the Z-Offsets between the channels, select
Lock Z.
• When locked, the movement of the Z-axis in one channel affects all
channels.
Note: Z-Offsets specify the optimal focus position in a channel relative to the
focus position in other channels. Setting the correct Z-Offsets is especially
important when the fluorescent markers in different channels are in different
focal planes.
display settings
Settings and Analysis Tools.
2. Click Image Display Settings button to expand the controls for image
display parameters for the selected channel.
3. Adjust Brightness , Contrast , and Gamma with the corresponding
sliders.
4. Click the Reset button to return the display settings to their default values.
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EVOS™ M5000 User Guide
Find the region of
1. Use the X-axis and Y-axis positioning knobs (page 6) to move the sample stage
2. To zoom in and out of the Viewing area,
image.
interest
and bring the region of interest into the field of view.
use the Zoom slider. The zoom range is
100% to 1000%.
3. If needed, re-adjust the brightness and focus, then proceed to capture the
Note: If desired, enable Quick Save (page 35) before proceeding to image capture.
Quick Save function allows you to set the save options in advance and save your
captured images with a single click directly from the Capture tab or automatically
after each capture.
EVOS™ M5000 User Guide
19
Capture image in a
1. Ensure that the Channel you want to capture is selected.
2. Click Capture to acquire the image.
single channel
When you select a Channel, the corresponding Capture channel box is
automatically checked.
Note: If you have exited the Live mode by clicking the Channel button again,
the current channel remains selected, as indicated by the blue line
underneath the corresponding channel button.
The viewing area shows the newly captured image.
A thumbnail of the captured image is displayed for the selected channel (in this
example, DAPI).
IMPORTANT! Captured images are stored in the memory buffer. If unsaved, newly
captured images overwrite the previously captured image in the selected channel.
Images captured in other fields and channels are not affected.
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EVOS™ M5000 User Guide
3. To capture the same field of view in another channel, select the desired
Channel.
4. If needed, re-adjust the brightness and focus, then click Capture.
The viewing area shows the image captured in the new channel superimposed
on the image captured in the previous channel.
A thumbnail of the image captured in the new channel is displayed along with
the thumbnail of the image from the previously captured channel.
EVOS™ M5000 User Guide
21
Capture multiple
1. To capture multiple channels simultaneously, select the desired channels by
channels
checking corresponding boxes.
simultaneously
2. If needed, adjust brightness and focus for each of the selected channels as
described.
3. Click Capture to acquire an image in each of the selected channels.
The Viewing area shows a multicolor overlay of the images captured in each
selected channel.
A thumbnail of the captured image is displayed for each selected channel (in
this example, DAPI, GFP, and Texas Red).
IMPORTANT! Captured images are stored in the memory buffer. If unsaved, newly
captured images overwrite the previously captured image in the selected channel.
Images captured in other fields and channels are not affected.
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EVOS™ M5000 User Guide
Analyze and annotate captured images
Display settings
Display settings and analysis tools allow you to change image display settings for
2. Click a button to open the corresponding tool; click the button again to close it.
Configure display
1. Click the Image Display Settings button to expand the controls for
or for a single channel in the
2. Optional: To remove a channel from displaying in the Viewing Area, unselect
5. Click the Image Display settings button again to collapse the controls.
and analysis tools
settings
live and captured images in the Viewing area, and to analyze and annotate
captured images.
1. Hover the pointer over the Viewing area to reveal the buttons for Display
Settings and Analysis Tools.
IMPORTANT! Changes made to images in the Viewing area with the display
settings and analysis tools, including changes made to image parameters, display
of the grid and the scale bar, as well as any annotations and measurements, persist
when the images are saved. If you want to save raw image data that you can use
for analysis, make sure to select Save individual channels option when saving
captured images (see page 34).
image display parameters (brightness, contrast, gamma) for captured
images.
Note: The controls for image display settings are contextual; they are
available only for channels with captured images
Live mode (with the excitation light turned on). In the example above, only
the controls for DAPI, GFP, and TX Red channels are displayed.
the corresponding checkbox.
To display a channel with a captured image that is not shown in the Viewing
Area, re-select the checkbox.
3. Adjust the Brightness , Contrast , and Gamma settings for each of
the selected channels using the corresponding sliders.
4. Click the Reset button to return the image display settings to their default
values.
EVOS™ M5000 User Guide
23
Display grid
1. Click the Grid button to superimpose a grid over the Viewing area.
4. Click the Grid Settings button again to save your settings and close the tool.
Display scale bar
1. Click the Scale Bar button to superimpose a scale bar over the
settings and close the tool.
2. To change the grid size, click the Grid
Settings button (the arrow on the Grid split
button) to open the Grid Settings tool.
3. Select the Size for the grid:
200 µm× 200 µm or 500 µm× 500 µm.
Viewing area.
2. To change scale bar settings, click the Scale Bar
Settings button (the arrow on the Scale Bar split
button) to open the Grid Settings tool.
3. Select Show End Bars to display the scale bar with the
end bars.
4. Select the Color for the scale bar.
5. Click the Scale Bar Settings button again to save your
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EVOS™ M5000 User Guide
Display histogram
1. Click the Histogram button to open the Intensity Histogram plot.
You can also close to plot by clicking the X on the plot.
2. Intensity Histogram plot shows the Pixel count vs. Intensity data of the image
displayed in the Viewing area.
3. Click the Histogram button again to close the Intensity Histogram plot.
EVOS™ M5000 User Guide
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