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Barnstead|Thermolyne Corporation
LOCATOR®& LOCATOR®Plus
Cryobiological Storage Vessel
OPERATION MANUAL
AND PARTS LIST
TYPE CY50900
Liquid Nitrogen Level Monitor
OPERATION MANUAL
AND PARTS LIST
TYPE CN50900
LT509X9 • 6/18/97
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Table of Contents
Table of Contents
Safety Information ................................................................................................................................3
Alert Boxes ............................................................................................................................................3
Please Read ..........................................................................................................................................3
General Usage ..................................................................................................................................3
Warnings ................................................................................................................................................4
General Specifications ..........................................................................................................................5
Environmental Conditions..................................................................................................................5
Declaration of Conformity ..................................................................................................................6
Unpackaging ..........................................................................................................................................7
Operation................................................................................................................................................8
Filling Instructions..............................................................................................................................8
Measuring Liquid Nitrogen Quantity ..................................................................................................8
Storing Samples in Vapor Phase ......................................................................................................9
General Cleaning Instructions............................................................................................................9
LOCATOR and LOCATOR Plus Replacement Parts ..........................................................................10
LOCATOR Accessories........................................................................................................................12
Cryo Accessories..................................................................................................................................13
Accessory Liquid Nitrogen Level Monitor Operation............................................................................14
Intended Use....................................................................................................................................14
General Usage ................................................................................................................................14
Function ..........................................................................................................................................14
Installation Procedure ..........................................................................................................................14
Level Monitor Remote Alarm ..........................................................................................................14
Connection to Remote Alarms ........................................................................................................15
Liquid Nitrogen Level Monitor’s Alarm Contact Specifications........................................................15
Principles of Operation ....................................................................................................................16
Operation of Level Monitor ..............................................................................................................18
Liquid Nitrogen Level Monitor Troubleshooting....................................................................................19
Level Monitor Replacement Parts List and Accessories......................................................................20
Ordering Procedures............................................................................................................................21
Warranty ..............................................................................................................................................23
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Safety Information
Your Thermolyne LOCATOR and LOCATOR Plus Series
have been designed with function, reliability and safety
in mind. For safe operation, please pay attention to the
alert boxes throughout the manual.
Please Read
All cryopreservation vessels, regardless of who manufactures them, use a vacuum to provide the super insulative properties needed to keep nitrogen in a liquid
form. They are very similar, in fact, to the thermos vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is
perfect or will last forever, we suggest that you monitor
the consumption of liquid nitrogen used by your vessel
on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly react to problems before they
arise. You may opt to use a level monitor for this purpose. (See page 7 for level monitor operation.).
Also, if your samples are super critical, one-of-a-kind, or
irreplaceable, consider allocating your samples to 2 separate vessels. Remember the old adage about placing
all your eggs into one basket….
Finally, because no vacuum will last forever, consider
having your vessel tested by Barnstead|Thermolyne or a
qualified cryovessel service technician every 7-10 years,
regardless of any problems (or lack thereof) you may
have had in the past. This will help insure your samples
against sudden loss of nitrogen due to vacuum failure.
General Usage
Do not use this product for anything other than its intended usage.
Safety Information
Warning
Warnings alert you to a
possibility of personal injury.
Caution
Cautions alert you to a
possibility of damage to the
equipment.
Note
Notes alert you to pertinent
facts and conditions.
Alert Signals
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WARNING
• Liquid Nitrogen is extremely cold; it boils at -196°C.
• To avoid injury due to frostbite or ruptured vials use extreme care whenever handling liquid nitrogen,
liquid nitrogen storage or transfer vessels, or any objects which have come in contact with liquid nitrogen.
• Leave no areas of skin exposed.
• Always wear proper safety attire over clothing: face shield, cryogenic gloves, cryogenic apron.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas from escaping.
• Always handle ampules with tongs. They may explode when removed from the vessel.
• Use extreme care to prevent spilling and splashing liquid nitrogen during transfer and removal of storage contents and holders.
• Immediately remove any clothing or safety attire on which liquid nitrogen has been spilled or splashed.
• Get immediate medical attention for any frostbite injuries due to liquid nitrogen.
CAUTION
• Never overfill liquid nitrogen vessels. Liquid nitrogen level should never be above the tops of
the racks inside the vessel. The excess weight
may crack the neck and result in vacuum failure.
• When inserting or removing racks be careful not to come in contact with the neck
tube area of the vessel. Remove or insert
racks slowly in a vertical manner.
Scratches on the neck tube area can cause
premature vacuum failure.
• Do not tamper with or remove vacuum port
(covered by a black plastic cap on side of vessel); this will void warranty.
• Do not spill liquid nitrogen on vacuum port this can cause vacuum failure.
• Always keep liquid nitrogen vessel in an upright
position.
Warning
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General Specifications
LOCATOR LOCATOR LOCATOR LOCATOR LOCATOR LOC ATOR LOCATOR
4 4 PLUS 6 PLUS 8 8 PLUS JR. JR. PLUS
HEIGHT 37.5” 40” 38” 37.5” 40” 27.5” 30”
(Including lid)1(95.25 cm) (101.6 cm) (96.5 cm) (95.25 cm) (101.6 cm) (69.85 cm) (76.2 cm)
DIAMETER 22” 22” 26” 22” 22” 22” 22”
(55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm)
VESSEL 110L 111L 165L 110L 111L 50L 51L
VOLUME
LN
2
90L 91L 134L 90L 91 40 41L
CAPACITY,
LIQUID PHASE
STORAGE
VAPOR PHASE 25L 26L 39L 25L 26L 20L 21L
LN2CAPACITY
STATIC LN
2
.65 .80 .80 .40 .58 .65 .80
EVAPORATION
RATE - L/DAY
LIQUID PHASE 2916 3240 4860 1800 2000 1296 1620
CAPACITY ampules* ampules* ampules ampules ampules ampules* ampules*
or 3600 or 4000 or 6000 or 1600 or 2000
ampules** ampules** ampules** ampules** ampules**
VAPOR PHASE 2268 2592 3888 1400 1600 972 ampules* 1296
CAPACITY ampules* ampules* or ampules* or ampules ampules or 1200 ampules*
or 3200 4800 ampules** or 1600
2800 ampules** ampules** ampules**
ampules**
1
The liquid nitrogen level monitor will add 1 inch to these heights. The transportation cart will add 4 inches
to these heights.
* Using Nalgene cryoboxes with a 9 x 9 ampule configuration.
** Using Nalgene System 100™ cryoboxes with a 10 x 10 ampule configuration. (Nalgene System 100™
ampules or equivalent are needed.)
Environmental Conditions
Operating: 17°C - 27°C; 20% to 80% relative humidity, non-condensing. Installation Category II
(over-voltage) in accordance with IEC 664. Pollution Degree 2 in accordance with IEC 664.
Altitude limit: 2,000 meters.
Storage: -25°C to 65°C; 10% to 85% relative humidity.
(Ampules actually
submerged in liquid
nitrogen.)
(Ampules are kept
slightly above the
level of liquid
mitrogen.)
General Specifications
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Declaration of Conformity
Barnstead|Thermolyne hereby declares under its sole responsibility that this product conforms with the technical requirements of the following standards:
EMC: EN 50081-1 Generic Emission Standard; EN 50082-1 Generic Immunity Standard;
Safety: IEC 1010-1-92 Safety requirements for electrical equipment for measurement,
control, and laboratory use; Part I: General Requirements
per the provisions of the Electromagnetic Compatability Directive 89/336/EEC, as amended by 92/31/EEC and
93/68/EEC, and per the provisions of the Low Voltage Directive 73/23/EEC, as amended by 93/68/EEC.
The authorized representative located within the European Community is:
European Manager
Barnstead|Thermolyne
Saarbrückener Str. 248
D-38116 Braunschweig
Germany
Copies of the Declaration of Conformity are available upon request.
Declaration of Conformity
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Unpackaging
Before using your new LOCATOR or LOCATOR Plus
vessel, carefully inspect the vessel prior to use. Check
for signs of damage which may have occurred in shipment. It is advisable to fill (See Filling Instructions) all
new units with liquid nitrogen and watch liquid nitrogen
loss rate for a few days. If there are any problems, call
Barnstead/Thermolyne as soon as possible.
The Warranty Registration card enclosed with the vessel
must be completed and returned to
Barnstead/Thermolyne within 30 days to make warranty
effective. This information must include the serial number, which is located on the label on the vessel.
NOTE
The most prevalent cause of failure of liquid nitrogen storage vessels is mechanical. The vessel
neck tube supports the full weight of the inner
shell and all the liquid nitrogen it contains. Aside
blow to the vessel causes the inner shell to swing
in a pendulum-like motion causing the neck tube
to be damaged. Any storage vessel which has
been in an accident, has been dropped or lowered
to hit on its side will tend to fail more rapidly than
one which has not.
CAUTION
Exercise caution when moving your LOCATOR or
LOCATOR PLUS vessel. LOCATOR and LOCATOR Plus cryobiological storage systems are not
transportation vessels. Transport carts are designed for mobility within the lab or lab to lab only.
Moving full vessels long distances, over cracks in
floor, thresholds, on inclined ramps or in elevators
can cause premature vacuum failure.
NOTE
If you must transport samples under cryogenic
conditions, consider the Arctic Express line of Dry
Shippers found in the Thermolyne catalog.
Unpacking
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Operation
Filling Instructions
To avoid damage to your LOCATOR or LOCATOR Plus
cryogenic storage vessel which may result in premature
vacuum loss, it is important that the following procedure be
used when adding liquid nitrogen to a warm vessel:
1. Add only a small amount of liquid nitrogen (5-10 liters)
to a new or warm vessel.
2. Allow this small amount of liquid nitrogen to sit in the
covered vessel for a minimum of 2 hours. This will limit
stress caused by the sudden temperature change associated with adding liquid nitrogen to a warm vessel.
3. Add an additional 15 liters of liquid nitrogen to the vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen consumption with a wooden yardstick or Level
Monitor.
5. Fill LOCATOR or LOCATOR Plus as indicated. (See
Caution on Page 7.) Remember to allow for displacement of liquid nitrogen when racks and boxes are inserted.
6. Insert and remove racks slowly. Allow liquid nitrogen to
run out of boxes and off of racks.
Measuring Liquid Nitrogen Quantity
1. Use a wooden yardstick to measure liquid nitrogen level. NEVER use a hollow tube or plastic dipstick to measure the liquid nitrogen level.
2. Level will be indicated by frost line which develops
when dipstick is removed.
3. Level Monitor can be used for constant measurements.
See page 7.
CAUTION
• Never overfill your LOCATOR or LOCATOR
Plus vessel with liquid nitrogen. The liquid
nitrogen level in your tank (with racks inserted) should never be above 20 inches (50
cm) for LOCATOR 4 and LOCATOR 8, 10
inches (25 cm) for LOCATOR Jr., 22 inches
(55.8 cm) for LOCATOR 4, 6 and 8 Plus and
12 inches for LOCATOR Jr. Plus. Filling the
tank up to or above the bottom of the neck
tube may cause immediate or premature
vacuum failure to occur.
• When inserting or removing racks, be careful not to come in contact with the neck tube
area of the vessel. Remove or insert racks
slowly in a vertical manner. Scratches on
the neck tube area can cause premature
vacuum failure.
• Do not spill liquid nitrogen on vacuum port
(covered by a black plastic cap on side of vessel) - this can cause vacuum failure.
Operation
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Storing Samples in Vapor Phase
1. Remove the 2 bottom-most cryoboxes from each
rack.
2. Measure the height of 2 cryoboxes stacked on top of
each other.
3. Fill vessel with liquid nitrogen to the height obtained
from step 2 using a yardstick to measure liquid level
in vessel.
4. Place racks into vessel without the 2 bottom-most
cryoboxes.
— Samples are now above the liquid nitrogen and are
stored in the vapor phase.—
Important—A level monitor should always be used
when you store your samples in the vapor phase
because of the already low levels of liquid nitrogen.
Please refer to page 11 for the correct level monitor for
your system.
General Cleaning Instructions
Wipe exterior surfaces with lightly dampened cloth containing mild soap solution.
OPERATION
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LOCATOR and LOCATOR Plus Replacement Parts
Model # Description
Model # Description
CV509X12A Replacement cork and glue for LOCATOR 4, LOCATOR 4 Plus, Jr and Jr. Plus
CV509X11A Replacement cork and glue for LOCATOR 8 and LOCATOR 8 Plus.
CV509X41A-70 Replacement cork and glue for LOCATOR 6 Plus.
ADX31 Glue for corks.
LOCATOR and LOCATOR Plus Vessels and Covers
TANK ONLY
No Racks STANDARDCOVERS W/LEVELMONITOR
TYPE or covers COVERS 240V 120V 100V
LOCATOR JR. CS509X11A CV509X10 CN509X1 CN509X3 CN509X2
LOCATOR JR.
Plus CS509X20A-70 CV509X10 CN509X1-70 CN509X3-70 CN509X2-70
LOCATOR 4 CS509X12A CV509X10 CN509X4 CN509X6 CN509X5
LOCATOR 4
Plus CS509X21A-70 CX509X10 CN509X4-70 CN509X6-70 CN509X5-70
LOCATOR 6
Plus CS509X2A-70 CV509X40-70 CN509X11-70 CN509X10-70 CN509X12-70
LOCATOR 8 CS509X13A CV509X9 CN509X7 CN509X9 CN509X8
LOCATOR 8
Plus CS509X23A-70 CV509X9 CN509X7-70 CN509X9-70 CN509X8-70
All level monitors listed operate on 16 volts (DC) 0.2 amps and 3.2 watts.
NOTE
LOCATOR and LOCATOR Plus vessels include
racks and covers. However, these items may
also be ordered separately.
Replacement Parts
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LOCATOR and LOCATOR Plus Racks and Boxes
STANDARD RACK SPECIAL RACK
TYPE (1.8 ml VIALS) (5.0 ml VIALS)
RACK 1.8 ml 1.8 ml RACK 5.0 ml
PART NO. 81 or 100 25 AMPULE PART NO. 81 AMPULE BOX
AMPULE BOX BOX
LOCATOR JR HR509X15A CS509X15 X HR509X19A CS509X10
4/VESSEL 16/VESSEL 4/VESSEL 8/VESSEL
4/RACK 2/RACK
LOCATOR JR. HR509X15A-70 CS509X15 X HR509X19A-70 CS509X10
PLUS 4/VESSEL 20/VESSEL 4/VESSEL 8/VESSEL
5/RACK 2/RACK
LOCATOR 4 HR509X2A CS509X15 X HR509X20A CS509X10
4/VESSEL 36/VESSEL 4/VESSEL 20/VESSEL
9/RACK 5/RACK
LOCATOR 4 HR509X2A-70 CS509X15 X HR509X20A-70 CS509X10
PLUS 4/VESSEL 40/VESSEL 4/VESSEL 20/VESSEL
10/RACK 5/RACK
LOCATOR 6 HR509X31A-70 CS509X15 X HR509X32-70 CS509X10
PLUS 6/VESSEL 60/VESSEL 6/VESSEL 30/VESSEL
10/RACK 5/RACK
LOCATOR 8 HR509X1A X CS509X16 X X
8/VESSEL 72/VESSEL
9/RACK
LOCATOR 8 HR509X1A-70 X CS509X15 X X
PLUS 8/VESSEL 80/VESSEL
10/RACK
REPLACEMENT PARTS
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LOCATORAccessories
AY509X33 1.5 ml cryoampules (500 per case) for System 100TMboxes.
AY509X32 1.0 ml cryoampules (500 per case) for System 100
TM
boxes.
AY509X12 2.0 ml cryoampules (500 per case) will not fit in System 100™ boxes.
AY509X13 1.2 ml cryoampules (500 per case) will not fit in System 100™ boxes.
CS509X17 Nalgene plastic boxes (System 100™) for LOCATOR Jr. and LOCATOR Jr. Plus (for use with
System 100™ ampules or equiv., case of 20)
CS509X18 Nalgene plastic boxes (System 100™) for LOCATOR 4 and LOCATOR 4 Plus (for use with
System 100™ ampules or equiv., case of 40)
CS509X5 Nalgene plastic boxes for LOCATOR Jr. and LOCATOR Jr. Plus (case of 20) holds 81 ampules
per box.
CS509X4 Nalgene plastic boxes for LOCATOR 4 and LOCATOR 4 Plus (case of 40) holds 81 ampules
per box.
CS509X3 Nalgene plastic boxes for LOCATOR 8 and LOCATOR 8 Plus (case of 80) holds 25 ampules
per box.
CS509X10 Nalgene plastic 4" box for 5 ml ampules. (Fits LOCATOR 4, LOCATOR 4 Plus, LOCATOR Jr.
and LOCATOR Jr. Plus.) Holds 81 ampules per box.
AY509X1 Transportation cart (fits all LOCATORS except LOCATOR 6 Plus)
AY509X1-70 Transportation cart (LOCATOR 6 Plus).
AY509X18 Cryoclaw (AY509X19 Suction Cups(2) used to remove floating ampules and boxes
LT509X5 Logbook for LOCATOR 4, LOCATOR 4 Plus, LOCATOR Jr. and LOCATOR Jr. Plus.
LT509X6 Logbook for LOCATOR 8 and LOCATOR 8 Plus.
LOCATOR Accessories
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Cryo Accessories
Nalgene Cryogenic vial Closure Color Coders, polystryrene (Package of 100)
Model No. AY509X27 AY509X28 AY509X29 AY509X30 AY509X31
Color White Yellow Blue Green Red
Nalgene Cryogenic vials (ampules), polypropylene; high density polyethylene closure (Case of 500), will no
t
work with System 100™ cryoboxes. Call for part numbers or compatibility with current ampules.
Model No. AY509X13 AY509X12
Capacity, ml 1.2 2.0
O.D., mm/in. 13.5/0.5 13.5/0.5
Height, mm/in. 38.1/1.5 48.3/1.88
Cryo Accessories
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Accessory Liquid Nitrogen
Level Monitor Operation
Intended Use
Provides a constant indication of liquid nitrogen level and
alerts you to low level conditions through audible and visual
alarms. The accessory Liquid Nitrogen Level Monitor can
be wired to a remote alarm system, to alert you to problems
when you are not in your laboratory.
General Usage
Do not use this product for anything other than its intended
usage.
Function
Safeguards your precious samples by informing you when
your vessel needs filling and alerting you to excessive liquid
nitrogen loss conditions.
Installation Procedure
1. Remove the level monitor and LOCATOR or LOCATOR Plus cover from box.
2. Remove present cover from your LOCATOR or LOCATOR Plus and replace with your new level monitor and cover.
3. Check the electrical specifications on the power
supply for electrical data and plug into appropriately
grounded receptacle.
Level Monitor Remote Alarm
1. Disconnect the Liquid Nitrogen Level Monitor from
the power supply. Disconnect power to your alarm
system.
2. Remove the three screws securing the Liquid Nitrogen Level Monitor’s cover. Pull the cover up and
out of the way. (See Figure 1.)
Connection to Remote Alarms
When connected to your remote alarm system, the Liquid
NOTE
If the level monitor was purchased as part of a
LOCATOR or LOCATOR Plus system, skip to
step #3. If it is a retrofit for an existing LOCATOR or LOCATOR Plus, begin with step #1.
NOTE
Level monitor is shipped mounted directly to the
appropriate LOCATOR or LOCATOR Plus
cover.
Accessory Liquid Nitrogen
Level Monitor Operation
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Nitrogen Level Monitor will alert you to a problem even
when your laboratory is unoccupied. The Liquid Nitrogen
Level Monitor’s alarm contacts are activated at the same
time the Monitor’s audible alarm sounds (see Micropro-
cessor Functions).
3. Remove the black plug from the side of the cov-
er. Insert the wires for the remote alarm through
this hole.
4. Connect the remote alarm wires as shown in
Figures 1, 2 and 3.
5. Replace the Liquid Nitrogen Level Monitor’s cov-
er. Resupply power to the Liquid Nitrogen Level
Monitor and to your remote alarm system.
Liquid Nitrogen Level Monitor’s Alarm Contact Specifications
The Liquid Nitrogen Level Monitor’s alarm contact is a
Photomos type relay.
Contact Form: Type 1 form B
Load Voltage Maximum: 400 Volts
(peak)
Load Current Maximum: 120 mA
Typical On Resistance: Maximum 26 Ω
NOTE
The Liquid Nitrogen Level Monitor can be configured to present your alarm system with either a
normally open contact or a normally closed contact. Which mode is currently operative depends
on the placement of a single jumper on the circuit
board. For a normally open contact, the jumper
should be placed on J6. For a normally closed
contact, the jumper should be placed on J4. (See
Figure 1.) Verify that the jumper is set appropri-
ately for your remote alarm system.
ACCESSORY LIQUID NITROGEN LEVEL MONITOR OPERATION
Figure 1 Figure 2
Remote Alarm wires connect here
(Note polarity.)
1
1
1
For normally open contact, place jumper here.
For normally closed contact, place jumper here.
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Principles of Operation
The innovative LIQUID NITROGEN LEVEL MONITOR from
THERMOLYNE utilizes ultrasonic sound waves to sense
the level of liquid nitrogen inside a or LOCATOR Plus cryogenic storage vessel. Amicroprocessor control provides
continuous indication of the liquid nitrogen level inside the
LOCATOR or LOCATOR Plus. Audible and visual alarms
also provide an early warning of low level conditions.
ULTRASONIC sound waves sense the level of liquid nitrogen inside the LOCATOR or LOCATOR Plus vessel.
By using ultrasonic sound waves to sense the level
of liquid nitrogen, no physical probe is required to
sense the level of liquid nitrogen. Conventional devices use probes which must be in physical contact
with the liquid. This physical contact increases liquid nitrogen consumption. There is NO increase in
liquid nitrogen consumption by using the THERMOLYNE LEVEL MONITOR.
Microprocessor Functions:
5 segment LED indication of the liquid nitrogen level.
The LED display is graduated in 5 segments; Full,
3/4, 1/2, 1/4 and Empty, similar to a fuel gauge on
an automobile. The bright orange LED display provides quick and reliable visual indication of the level of liquid nitrogen inside the LOCATOR or LOCATOR Plus vessel.
Audible and Visual alarms:
1. “LOW LEVEL” indicator light flashes on
and off when 2" of liquid nitrogen remains
inside the LOCATOR or LOCATOR Plus
vessel. In this situation, the level of liquid
Figure 3
ACCESSORY LIQUID NITROGEN LEVEL MONITOR OPERATION
U4
1
2
3 6
The position of J4 and J6 indicate
whether the relay is normally open
or normally closed.
Remote Alarm Connections Schematic
4
5
J3-1
J3-2
V
L
Load
Load Voltage
(AC, DC)
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nitrogen is low, and you are alerted to
re-fill the vessel as soon as possible.
2. An AUDIBLE alarm will sound intermittently when 1" of liquid nitrogen remains
inside the LOCATOR or LOCATOR Plus
vessel. The “LOW LEVEL” light described above will continue to flash as
well. In this situation, the liquid nitrogen
is dangerously low, and you are alerted
to re-fill the vessel immediately.
3. The 5 segment LED display becomes lit
and an audible alarm sounds continuously when the LOCATOR or LOCATOR
Plus vessel has no more liquid nitrogen
or if the level inside the vessel is falling
at a rate of 1/2" per hour or more. In this
situation, you are alerted that an emergency exists. Either there is no more liquid nitrogen inside the LOCATOR or LOCATOR Plus vessel, or the level of liquid
nitrogen is dropping rapidly, due to a
vacuum failure of the LOCATOR or LOCATOR Plus vessel.
Safety Feature:
A “RESET” switch lights and an audible alarm
sounds intermittently if the user does not reset
the monitor after removing vessel cover. The microprocessor must be reset each time the cover
is removed from the LOCATOR or LOCATOR
Plus.
Before removing the cover, the reset button
must be pushed, illuminating the reset light.
While in this reset/hold mode, the monitor is not
sensing. After five minutes in the reset/hold
mode, an audible alarm will sound, alerting you
to deactivate the reset/hold mode and replace
the cover on the vessel.
IMPORTANT NOTE
The Level Monitor may take up to 10 minutes to
establish a reading after the unit is placed on
the cryovessel. Remember to press “Reset” after placing unit on the vessel. The green LED
on the switch should be “OFF.”
A
CCESSORY LIQUID NITROGEN LEVEL MONITOR OPERATION
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Operation of level monitor
Once the cover with the monitor has been placed on the vessel and plugged in, it will take the system about 15 minutes to
equilibrate before an accurate measurement of the liquid
nitrogen will be indicated. This equilibration process will occur
each time the vessel is used. Each time a vessel is opened,
the stabilized chamber temperature is disrupted. The system
requires a stable temperature to determine an accurate liquid
nitrogen level.
If you have just purchased a new LOCATOR or LOCATOR
Plus along with your monitor, allow at least 1 day for the liquid
nitrogen to equilibrate the vessel before plugging in the unit.
This will avoid false alarms.
ACCESSORY LIQUID NITROGEN LEVEL MONITOR OPERATION
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Liquid Nitrogen Level Monitor Troubleshooting
Problem Cause Solution
Level monitor alarm sounds Ice or frost buildup on the Remove level monitor from vessel
intermittently. Unit measuring transducer. and place in room for approximately
liquid level incorrectly. twenty minutes. Make sure lid is
standing upright on the cork to allow
moisture to drain out of level monitor.
Place level monitor back on unit.
(Remember to press “Reset” button.)
Level monitor measuring “Full” Fog in top part (neck area) of The density of the fog that is formed
after removing samples and cryogenic vessel. after removing the lid and accessing
replacing lid. samples is dependent on the humidity
of the room where the cryovessel is
found. The fog dissipates after about
fifteen minutes and at this time the
level monitor will take an accurate
reading of the liquid nitrogen level.
Liquid Nitrogen Level Monitor
Troubleshooting
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Level Monitor Replacement Parts List and Accessories
PART LOCATOR LOCATOR LOCATOR LOCATOR LOCATOR LOCATOR LOCATOR
JR JR PLUS 4 4 PLUS 6 PLUS 8 8 PLUS
Liquid Nitrogen CN509X3-120V CN509X3-70-120V CN509X6-120V CN509X6-70-120V CN509X10-70-120V CN509X9-120V CN509X9-70-120V
Level Monitor CN509X2-100V CN509X2-70-100V CN509X5-100V CN509X5-70-100V CN509X12-70-100V CN509X8-100V CN509X8-70-100V
CN509X1-240V CN509X1-70-240V CN509X4-240V CN509X4-70-240V CN509X11-70-240V CN509X7-240V CN509X7-70-240V
1. *Transducer CV509X21B CV509X21B CV509X21B CV509X21B CV509X21B CV509X20B CV509X20B
2. P.C. Board
(Bottom Board) PC509X1A PC509X4A PC509X3A PC509x5A PC509X5A PC509X3A PC509X5A
3. Display Board
(Top Board) PC509X2B PC509X2B PC509X2B PC509X2B PC509X2B PC509X2B PC509X2B
4. Single bar
LED SCX113 SCX113 SCX113 SCX113 SCX113 SCX113 SCX113
5. Four Bar
LED SCX114 SCX114 SCX114 SCX114 SCX114 SCX114 SCX114
6. Cover CV509X13 CV509X13 CV509X13 CV509X13 CV509X13 CV509X13 CV509X13
7. Power
Supply/120V TN509X2 TN509X2 TN509X2 TN509X2 TN509X2 TN509X2 TN509X2
8. Power
Supply/100V TN509X1 TN509X1 TN509X1 TN509X1 TN509X1 TN509X1 TN509X1
9. Power
Supply/240V TN509X3 TN509X3 TN509X3 TN509X3 TN509X3 TN509X3 TN509X3
*Will include cover, cork, transducer and aluminum bottom of control section. Does not include items 2-9 listed
above.
Level Monitor Replacement
Parts List and Accessories
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Ordering Procedures
Ordering Procedures
Please refer to the Specification Plate for the complete
model number, serial number, and series number when
requesting service, replacement parts or in any correspondence concerning this unit.
All parts listed herein may be ordered from the
Barnstead|Thermolyne dealer from whom you purchased this unit.
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Barnstead|Thermolyne
Two Year Limited Warranty for Locator
Cryobiological Storage Vessels and
One Year Limited Warranty for Liquid Nitrogen Level
Monitors
Barnstead|Thermolyne Corporation warrants this product to be free from defects in mate-
rial and construction for a period of two years from date of purchase for the Locator cryovessel and one year from date of purchase for the level monitor.
This warranty applies only to defects in original parts or components, and does not apply to
claims or alleged product failures resulting from unauthorized repairs, misuse, accidents or
lack of proper maintenance, failure to follow Barnstead|Thermolyne’s instructions for use
or from ordinary wear and tear.
Warranty service may be obtained by returning any defective product to an authorized
Barnstead|Thermolyne dealer or to Barnstead|Thermolyne.
Barnstead|Thermolyne
P.O. Box 797
2555 Kerper Boulevard
Dubuque, IA 52004-0797
USA
FAX: (319) 589-0516 • (319) 589-0530
E-Mail: mkt@barnsteadthermolyne.com
Barnstead|Thermolyne’s sole obligation with respect to its product shall be to repair or
replace the product. Under no circumstances shall it be liable for incidental or consequential
damage.
THE WARRANTY STATED HEREIN IS THE SOLE WARRANTY APPLICABLE TO
BARNSTEAD|THERMOLYNE PRODUCTS. BARNSTEAD|THERMOLYNE EXPRESSLY
DISCLAIMS ANYAND ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR FITNESS FOR USE.
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Barnstead Thermolyne
2555 Kerper Blvd.
P.O. Box 797
Dubuque, IA 52004-0797 USA
PHONE: 319-556-2241 • 800-553-0039
FAX: 319-589-0516
E-Mail: mkt@barnsteadthermolyne.com
a subsidiary of
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