Thermo Scientific CY50925, CY50945, CY50935 Instruction Manual
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Barnstead|Thermolyne Corporation
LOCATOR®& LOCATOR®Plus
Cryobiological Storage Vessel
OPERATION MANUAL
AND PARTS LIST
TYPE CY50900
Liquid Nitrogen Level Monitor
OPERATION MANUAL
AND PARTS LIST
TYPE CN50900
LT509X9 • 6/18/97
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Table of Contents
Table of Contents
Safety Information ................................................................................................................................3
Alert Boxes ............................................................................................................................................3
Please Read ..........................................................................................................................................3
General Usage ..................................................................................................................................3
Warnings ................................................................................................................................................4
General Specifications ..........................................................................................................................5
Environmental Conditions..................................................................................................................5
Declaration of Conformity ..................................................................................................................6
Unpackaging ..........................................................................................................................................7
Operation................................................................................................................................................8
Filling Instructions..............................................................................................................................8
Measuring Liquid Nitrogen Quantity ..................................................................................................8
Storing Samples in Vapor Phase ......................................................................................................9
General Cleaning Instructions............................................................................................................9
LOCATOR and LOCATOR Plus Replacement Parts ..........................................................................10
LOCATOR Accessories........................................................................................................................12
Cryo Accessories..................................................................................................................................13
Accessory Liquid Nitrogen Level Monitor Operation............................................................................14
Intended Use....................................................................................................................................14
General Usage ................................................................................................................................14
Function ..........................................................................................................................................14
Installation Procedure ..........................................................................................................................14
Level Monitor Remote Alarm ..........................................................................................................14
Connection to Remote Alarms ........................................................................................................15
Liquid Nitrogen Level Monitor’s Alarm Contact Specifications........................................................15
Principles of Operation ....................................................................................................................16
Operation of Level Monitor ..............................................................................................................18
Liquid Nitrogen Level Monitor Troubleshooting....................................................................................19
Level Monitor Replacement Parts List and Accessories......................................................................20
Ordering Procedures............................................................................................................................21
Warranty ..............................................................................................................................................23
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Safety Information
Your Thermolyne LOCATOR and LOCATOR Plus Series
have been designed with function, reliability and safety
in mind. For safe operation, please pay attention to the
alert boxes throughout the manual.
Please Read
All cryopreservation vessels, regardless of who manufactures them, use a vacuum to provide the super insulative properties needed to keep nitrogen in a liquid
form. They are very similar, in fact, to the thermos vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is
perfect or will last forever, we suggest that you monitor
the consumption of liquid nitrogen used by your vessel
on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly react to problems before they
arise. You may opt to use a level monitor for this purpose. (See page 7 for level monitor operation.).
Also, if your samples are super critical, one-of-a-kind, or
irreplaceable, consider allocating your samples to 2 separate vessels. Remember the old adage about placing
all your eggs into one basket….
Finally, because no vacuum will last forever, consider
having your vessel tested by Barnstead|Thermolyne or a
qualified cryovessel service technician every 7-10 years,
regardless of any problems (or lack thereof) you may
have had in the past. This will help insure your samples
against sudden loss of nitrogen due to vacuum failure.
General Usage
Do not use this product for anything other than its intended usage.
Safety Information
Warning
Warnings alert you to a
possibility of personal injury.
Caution
Cautions alert you to a
possibility of damage to the
equipment.
Note
Notes alert you to pertinent
facts and conditions.
Alert Signals
4
WARNING
• Liquid Nitrogen is extremely cold; it boils at -196°C.
• To avoid injury due to frostbite or ruptured vials use extreme care whenever handling liquid nitrogen,
liquid nitrogen storage or transfer vessels, or any objects which have come in contact with liquid nitrogen.
• Leave no areas of skin exposed.
• Always wear proper safety attire over clothing: face shield, cryogenic gloves, cryogenic apron.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas from escaping.
• Always handle ampules with tongs. They may explode when removed from the vessel.
• Use extreme care to prevent spilling and splashing liquid nitrogen during transfer and removal of storage contents and holders.
• Immediately remove any clothing or safety attire on which liquid nitrogen has been spilled or splashed.
• Get immediate medical attention for any frostbite injuries due to liquid nitrogen.
CAUTION
• Never overfill liquid nitrogen vessels. Liquid nitrogen level should never be above the tops of
the racks inside the vessel. The excess weight
may crack the neck and result in vacuum failure.
• When inserting or removing racks be careful not to come in contact with the neck
tube area of the vessel. Remove or insert
racks slowly in a vertical manner.
Scratches on the neck tube area can cause
premature vacuum failure.
• Do not tamper with or remove vacuum port
(covered by a black plastic cap on side of vessel); this will void warranty.
• Do not spill liquid nitrogen on vacuum port this can cause vacuum failure.
• Always keep liquid nitrogen vessel in an upright
position.
Warning
5
General Specifications
LOCATOR LOCATOR LOCATOR LOCATOR LOCATOR LOC ATOR LOCATOR
4 4 PLUS 6 PLUS 8 8 PLUS JR. JR. PLUS
HEIGHT 37.5” 40” 38” 37.5” 40” 27.5” 30”
(Including lid)1(95.25 cm) (101.6 cm) (96.5 cm) (95.25 cm) (101.6 cm) (69.85 cm) (76.2 cm)
DIAMETER 22” 22” 26” 22” 22” 22” 22”
(55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm) (55.8 cm)
VESSEL 110L 111L 165L 110L 111L 50L 51L
VOLUME
LN
2
90L 91L 134L 90L 91 40 41L
CAPACITY,
LIQUID PHASE
STORAGE
VAPOR PHASE 25L 26L 39L 25L 26L 20L 21L
LN2CAPACITY
STATIC LN
2
.65 .80 .80 .40 .58 .65 .80
EVAPORATION
RATE - L/DAY
LIQUID PHASE 2916 3240 4860 1800 2000 1296 1620
CAPACITY ampules* ampules* ampules ampules ampules ampules* ampules*
or 3600 or 4000 or 6000 or 1600 or 2000
ampules** ampules** ampules** ampules** ampules**
VAPOR PHASE 2268 2592 3888 1400 1600 972 ampules* 1296
CAPACITY ampules* ampules* or ampules* or ampules ampules or 1200 ampules*
or 3200 4800 ampules** or 1600
2800 ampules** ampules** ampules**
ampules**
1
The liquid nitrogen level monitor will add 1 inch to these heights. The transportation cart will add 4 inches
to these heights.
* Using Nalgene cryoboxes with a 9 x 9 ampule configuration.
** Using Nalgene System 100™ cryoboxes with a 10 x 10 ampule configuration. (Nalgene System 100™
ampules or equivalent are needed.)
Environmental Conditions
Operating: 17°C - 27°C; 20% to 80% relative humidity, non-condensing. Installation Category II
(over-voltage) in accordance with IEC 664. Pollution Degree 2 in accordance with IEC 664.
Altitude limit: 2,000 meters.
Storage: -25°C to 65°C; 10% to 85% relative humidity.
(Ampules actually
submerged in liquid
nitrogen.)
(Ampules are kept
slightly above the
level of liquid
mitrogen.)
General Specifications
6
Declaration of Conformity
Barnstead|Thermolyne hereby declares under its sole responsibility that this product conforms with the technical requirements of the following standards:
EMC: EN 50081-1 Generic Emission Standard; EN 50082-1 Generic Immunity Standard;
Safety: IEC 1010-1-92 Safety requirements for electrical equipment for measurement,
control, and laboratory use; Part I: General Requirements
per the provisions of the Electromagnetic Compatability Directive 89/336/EEC, as amended by 92/31/EEC and
93/68/EEC, and per the provisions of the Low Voltage Directive 73/23/EEC, as amended by 93/68/EEC.
The authorized representative located within the European Community is:
European Manager
Barnstead|Thermolyne
Saarbrückener Str. 248
D-38116 Braunschweig
Germany
Copies of the Declaration of Conformity are available upon request.
Declaration of Conformity
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Unpackaging
Before using your new LOCATOR or LOCATOR Plus
vessel, carefully inspect the vessel prior to use. Check
for signs of damage which may have occurred in shipment. It is advisable to fill (See Filling Instructions) all
new units with liquid nitrogen and watch liquid nitrogen
loss rate for a few days. If there are any problems, call
Barnstead/Thermolyne as soon as possible.
The Warranty Registration card enclosed with the vessel
must be completed and returned to
Barnstead/Thermolyne within 30 days to make warranty
effective. This information must include the serial number, which is located on the label on the vessel.
NOTE
The most prevalent cause of failure of liquid nitrogen storage vessels is mechanical. The vessel
neck tube supports the full weight of the inner
shell and all the liquid nitrogen it contains. Aside
blow to the vessel causes the inner shell to swing
in a pendulum-like motion causing the neck tube
to be damaged. Any storage vessel which has
been in an accident, has been dropped or lowered
to hit on its side will tend to fail more rapidly than
one which has not.
CAUTION
Exercise caution when moving your LOCATOR or
LOCATOR PLUS vessel. LOCATOR and LOCATOR Plus cryobiological storage systems are not
transportation vessels. Transport carts are designed for mobility within the lab or lab to lab only.
Moving full vessels long distances, over cracks in
floor, thresholds, on inclined ramps or in elevators
can cause premature vacuum failure.
NOTE
If you must transport samples under cryogenic
conditions, consider the Arctic Express line of Dry
Shippers found in the Thermolyne catalog.
Unpacking
8
Operation
Filling Instructions
To avoid damage to your LOCATOR or LOCATOR Plus
cryogenic storage vessel which may result in premature
vacuum loss, it is important that the following procedure be
used when adding liquid nitrogen to a warm vessel:
1. Add only a small amount of liquid nitrogen (5-10 liters)
to a new or warm vessel.
2. Allow this small amount of liquid nitrogen to sit in the
covered vessel for a minimum of 2 hours. This will limit
stress caused by the sudden temperature change associated with adding liquid nitrogen to a warm vessel.
3. Add an additional 15 liters of liquid nitrogen to the vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen consumption with a wooden yardstick or Level
Monitor.
5. Fill LOCATOR or LOCATOR Plus as indicated. (See
Caution on Page 7.) Remember to allow for displacement of liquid nitrogen when racks and boxes are inserted.
6. Insert and remove racks slowly. Allow liquid nitrogen to
run out of boxes and off of racks.
Measuring Liquid Nitrogen Quantity
1. Use a wooden yardstick to measure liquid nitrogen level. NEVER use a hollow tube or plastic dipstick to measure the liquid nitrogen level.
2. Level will be indicated by frost line which develops
when dipstick is removed.
3. Level Monitor can be used for constant measurements.
See page 7.
CAUTION
• Never overfill your LOCATOR or LOCATOR
Plus vessel with liquid nitrogen. The liquid
nitrogen level in your tank (with racks inserted) should never be above 20 inches (50
cm) for LOCATOR 4 and LOCATOR 8, 10
inches (25 cm) for LOCATOR Jr., 22 inches
(55.8 cm) for LOCATOR 4, 6 and 8 Plus and
12 inches for LOCATOR Jr. Plus. Filling the
tank up to or above the bottom of the neck
tube may cause immediate or premature
vacuum failure to occur.
• When inserting or removing racks, be careful not to come in contact with the neck tube
area of the vessel. Remove or insert racks
slowly in a vertical manner. Scratches on
the neck tube area can cause premature
vacuum failure.
• Do not spill liquid nitrogen on vacuum port
(covered by a black plastic cap on side of vessel) - this can cause vacuum failure.
Operation
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