Thermo Scientific Cryotome Operator's Manual

77210163GB Issue 3
STANDARD AND ELECTRONIC
OPERATOR
GUIDE
77210163
Standard & E
ENGLISH
Cryotome
©
77210163GB Issue 3
2
Thermo Fisher Scientific makes every endeavour to ensure that the information contained in its support documentation is correct and clearly stated but does not accept responsibility for any errors or omissions. The development of Thermo products and services is continuous. Make sure that any published information that you use for reference is up to date and relates to the status
of the product. If necessary, check with Thermo or your local Thermo representative.
© 2009 Thermo Fisher Scientific. All rights reserved.
This manual may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any
electronic or machine readable form without prior written consent of Thermo
SYMBOLS
The following symbols and conventions are used throughout this manual and on the instrument.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT, TO WARN THAT INSTRUCTIONS MUST BE FOLLOWED FOR SAFE AND CORRECT OPERATION.
IF THIS SYMBOL APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT, TO WARN THAT A HAZARD MAY EXIST DUE TO AN EXTREME OF TEMPERATURE. IF THIS SYMBOL
APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT, TO WARN THAT THERE MAY BE A BIOHAZARD ASSOCIATED WITH THE INSTRUMENT. ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF THE SAMPLES USED. TAKE SUITABLE PRECAUTIONS
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT, TO WARN THAT HARMFUL CHEMICALS ARE USED WITH THE INSTRUMENT. REFER TO THE MATERIAL SAFETY DATA SHEETS FOR THE CHEMICALS USED. ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF LOCAL LABORATORY PROCEDURES. TAKE SUITABLE PRECAUTIONS
A warning is given in the document if there is a danger of personal injury or damage to samples or equipment
Note
1 Notes give more information about a job or instruction but do not form part of
the instruction
WARNING
The Cryotome range meets the following CE Mark requirements:
In Vitro Diagnostic Directive 98/79/EC
Low Voltage Directive 73/23/EEC, as amended by 93/68/EEC.
®
C US
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LIST OF CONTENTS
CONTENTS This Page
WELCOME 4
Introduction; Safety
DESCRIPTION 7
Overview
CONTROLS 8
Description; Main Control Panel; Connections;
INSTALLATION AND SETTING UP 21
Introduction; To Unpack; To Set Up the Cryotome; Electrical Requirements;
OPERATION 40
Description, Safety Considerations, Precautions.
CLEANING AND MAINTENANCE 47
Introduction; Routine Cleaning and Maintenance Instructions
TROUBLE SHOOTING 53
Introduction; Instrument Function; Sectioning Problem Solutions
SPECIFICATION AND ACCESSORIES 57
WARRANTY STATEMENT 60
Warranty Statement; Declaration of Conformity
INDEX 62
APPENDIX A - TRANSPORTATION INSTRUCTIONS 65
To Repackage the Instrument; Product Return Safety Declaration
APPENDIX B - APPROVED REAGENT LIST 69
APPENDIX C - EXAMPLES 70
Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company
Cryotome®,
Cryomatrix™ and Cryochrome™
are trade marks of Thermo Shandon Limited
Thermo Fisher Scientific is the trading name of Thermo Shandon Limited
All information contained in this manual is proprietary and confidential, and the exclusive property of Thermo Fisher Scientific. This manual is protected by copyright and any reproduction is prohibited. This manual is for use only by the individuals to which it has been made available by Thermo Fisher Scientific.
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WELCOME
1 INTRODUCTION
Welcome to the Cryotome Electronic (E) and standard cryostats. The Cryotome series of cryostats is designed for the routine sectioning of quick-frozen tissue specimens embedded in a medium, for subsequent preparation and diagnosis by a pathologist. The Cryotome series of cryostats are intended for use in Pathology Laboratories by appropri­ately trained Medical Laboratory Technicians
.
Designed and made with care, the instrument is safe to use, simple to operate, and easy to maintain. The Cryotome series of cryostats conforms with IEC1010-1 the safety standard for laboratory instruments.
This Operator Guide gives instructions for the correct operation and use of the Cryotome E and Cryotome
. Instructions and information that applies only to a particular version of Cryotome are marked [E only] for Electronic Cryotomes, or [Std only] for Standard Cryotomes.
2 SAFETY
THIS PARAGRAPH DETAILS IMPORTANT SAFETY INFORMATION. PLEASE READ THIS SECTION CAREFULLY.
Thermo products are designed for convenient and reliable operation and to
accepted
standards of safety.
The use of the Cryotome does not entail any hazard if operated in accordance with the instructions given in this manual. However, incorrect actions by a user may damage the equipment, or cause a hazard to health. It is important for you to obey the following safety precautions:
i All users must read and understand the Operator Guide and only operate
the unit in accordance with the instructions. If the instructions are not followed, then the protection provided by the instrument may be impaired.
ii Do not modify the instrument - if unauthorised modifications are carried
out, the instrument may be made unsafe and the warranty may be invalidated.
iii Potentially lethal voltages above 110V a.c. or 50V d.c. are present inside the
instrument.
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`iv This instrument has a protective earth system and must be properly
connected to a good earth (Ground) via the mains input supply.
v Do not remove any panels or covers unless specifically instructed to
do so. Cryotome does not have any user serviceable parts inside the instrument.
vi It is important that normal standards of safety and good laboratory
practices are employed. Always use common sense and the best known practice when operating the instrument.
vii Sharp knives and blades are used in this instrument. Make sure you
understand the correct methods for their fixing and use.
viii The Centre of Gravity position for the Cryotome is shown below:
221 mm
508 mm
317 mm
WINDOW BOOST
ix The Cryotome weighs approximately 120 kilograms (265 lbs) when empty;
get help to move it.
x If the instrument has been used with materials that are toxic or
contaminated with pathogenic micro-organisms, follow your laboratory guidelines and the fumigation and cleaning instructions given in Chapter 5 of this Operator Guide. The Product Return Certificate (in Appendix A) must be completed if the instrument is to be returned to Thermo or serviced by a Thermo trained engineer.
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xi The instrument should be regularly cleaned as described in Chapter 5 of
this Operator Guide. Refer to Material Safety Data Sheets when dealing with the reagents specified in Appendix B.
xii
Do not use the Cryotome for general refrigeration purposes, such as
storage of specimens. Use a conventional freezer.
xiii Use only Thermo approved accessories or replacement parts with the
Cryotome.
xiv Correct maintenance procedures are essential to maintain safe operation
and for consistent performance. It is recommended that a Maintenance Contract is taken out with your supplier.
xv The instrument must be serviced annually by a Thermo trained engineer
in accordance with the instructions contained in the Cryotome Service Manual (77210166).
xvi Any problems and queries should be referred to your supplier.
xvii Refrigeration System
The Cryotome uses R404A refrigerant
THE REFRIGERATION SYSTEM MUST ONLY BE WORKED ON BY SUITABLY QUALIFIED ENGINEERS
REFRIGERANTS ARE GREENHOUSE GASSES AND ALL PRECAUTIONS MUST BE TAKEN TO RECOVER ANY GAS REMOVED FROM UNITS AND TO PREVENT ITS RELEASE INTO THE ATMOSPHERE
ALWAYS USE THE CORRECT REFRIGERANT TO CHARGE THE INSTRUMENT AND ALWAYS DISPOSE OF ANY USED REFRIGERANT IN ACCORDANCE WITH LOCAL AND NATIONAL REGULATIONS
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DESCRIPTION
1.1 CRYOTOME OVERVIEW
The Cryotome is designed for cutting frozen sections of fixed and unfixed specimens, rapidly and accurately, for future examination by microscope. It operates by rapidly freezing the sample in a temperature controlled environment, and provides means for sectioning the frozen sample by conventional microtome.
The different components of the Cryotome are shown in the diagram.
a Micron Selector [E only]
b
S p e c i m e n Trav e l Ava i l a bl e Indicator
c Time Controls
d Chamber Controls
e Temperature Controls
f Keyswitch
g Handwheel
h Instrument Lowering Knobs
i Waste Bottle
j Advance/rewind controls [E only]
Micron Selector / Advance/rewind
controls [Std only]
For optimum performance, the microtome Specimen Head and Knife Holder are held within the refrigerated chamber. The main body of the microtome is mounted outside the chamber to give more space for working in, and for easier cleaning.
Principal factors for good sectioning of frozen specimens are:
i the temperature must be correct for the specimen being cut.
ii the microtome must be correctly adjusted and operated.
iii the cutting blade must be sharp and set at the correct angle.
iv the anti-roll plate must be correctly adjusted.
a
i
j
g
f
edcb
h
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CONTROLS
2.1 DESCRIPTION
This chapter describes the displays and the functions of all the controls on the instrument.
2.2 MAIN CONTROL PANEL
The Main Control Panel is situated on the front of the instrument (a). Operation of the buttons is confirmed by an audible tone, a change in the display, or by an integral pushbutton indicator. Display panels show instrument status and error messages.
Note
1 There is no ‘feel’ when a switch operates.
The Main Control Panel is divided into five sections.
2.2.1 MICRON SELECTOR [E only]
The Micron Selector section of the Control Panel displays the thickness in microns of the sections being taken.
The number in the display is changed by pressing the [+] and [-] buttons. Press [+] to increase the thickness by 1 (if the number is in the range 0 - 19), or 5 (if the number is in the range 20 - 55). Press
[-] to decrease the thickness by the same amounts.
Note
1 A dot in the left hand side of the display (i) indicates that the Specimen Head
is moving in the REWIND direction. A dot to the left of the right hand digit in the display (ii) indicates that the Specimen Head is moving in the ADVANCE direction.
iii
a
MICRON SELECTOR
+
-
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2 The Micron Selector on the Standard Cryotome is a rotary knob which changes
the thickness in 1µm steps between 1 and 30µm (see section 2.3.1)
2.2.2 SPECIMEN TRAVEL
The vertical bar graph contains separate segments that light up sequentially as the Specimen Head advances from its maximum available travel position. The proportion of lit to unlit segments indicates the amount of travel still available at any time.
SPECIMEN TRAVEL AVAILABLE
RESET
MAX
MIN
SPECIMEN TRAVEL AVAILABLE
MAX
MIN
[E only] [Std only]
When the instrument is first powered up, the first segment flashes to indicate that the Specimen Head is in its maximum available travel (MAX) position. As the Specimen Head advances at the end of each cutting stroke, the segments of the display light one by one until eventually all the segments are lit when no more travel is available. An audible alarm operates when the end of travel is reached.
[E only]: Press [RESET] to return the Specimen Head to its maximum available travel position. The button LED will light and the display will flash during the reset and the segments will count down until the Specimen Head reaches maximum travel. The first segment will flash.
Note
1 When the instrument is being reset, all other advance or rewind functions are
over-ridden. This occurs each time the instrument is switched on, or [RESET] is pressed.
2.2.3 TIME CONTROLS
The Time Controls allow you to set the clock time, defrost times and fumigate times. Real time is displayed in 24 hour clock format, and a flashing colon (:) shows that operation is normal.
CLOCK
TIME CONTROLS
DAY
HOUR MINUTE
DEFROST
FUMIGATE
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2.2.3.1 TO SET THE CLOCK
Press and hold [CLOCK] and then press the relevant button detailed below. Release the buttons when the correct information is displayed.
To Set the... Press and hold [CLOCK] then press: Display will show:
Day [DAY] 1 - 7
Note Keep Monday as Day 1 to avoid confusion
Hours [HOURS] 0 - 23
Minutes [MINUTES] 1 - 59
2.2.3.2 TO SET DEFROST TIMES
Press and hold [DEFROST] and then press the relevant button detailed below. Release the buttons when the correct information is displayed.
To Set the... Press and hold [DEFROST] then
press:
Display will show:
Day [DAY] 0 - 9 (see note 1 below)
Hours [HOURS] 0 - 23
Minutes [MINUTES] 1 - 59
Note:
1 Use the following settings for the days:
DAY 0 = prevents the programmed function. DAY 1-7 = defrosting starts at the time set on the day shown (set
Monday as Day 1 to avoid confusion). DAY 8 = defrosting starts at the set time every day DAY 9 = STANDBY (PSAVE = power save)
2 Standby (PSAVE) allows the instrument to remain switched on in a defrosted
state for up to 24 hours until a pre-determined time. It then returns to its normal operational state
To set Standby (PSAVE) mode, press and hold [DEFROST] then press [DAY] and select day 9. Set the time that normal operation is required to resume. Release the buttons.
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To start (PSAVE) mode immediately, press [IMMED DEFROST]. The instrument then runs its defrost cycle for 15 mins and goes to (PSAVE) on completion. Use [CANCEL DEFROST/ FUMIGATE] to end the function before the cycle is complete.
Note:
1 It can take 1 - 2 hours after (PSAVE) mode to cool the Refrigerated Chamber
sufficiently for use. Make allowance for this when setting the resume time.
2.2.3.3 TO SET FUMIGATE TIMES
Follow the instructions in 2.2.3.2 (To Set Defrost Times), but press [FUMIGATE] instead of [DEFROST].
To start (PSAVE) mode, press [IMMED FUMIGATE]. The instrument then starts the 5 hour Fumigate cycle before going into (PSAVE).
2.2.4 CHAMBER CONTROLS
CHAMBER CONTROLS
CRYOBAR
BOOST
WINDOW
LOCK
LAMP
CRYOBAR
TEMP
WORKING
TEMP
IMMED.
DEFROST
IMMED.
FUMIGATE
CANCEL DEFROST/ FUMIGATE
TEMPERATURE °C
+ -
2.2.4.1 [IMMED DEFROST]
Press [IMMED DEFROST] to defrost the refrigeration system. In addition:
i the compressor switches on,
ii Cryobar Boost is switched off,
iii [IMMED DEFROST] is lit,
iv dEF shows in the temperature display.
v After 5 minutes the defrost heater and compressor switch off for a further 10
minutes.
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Defrosting stops after 15 minutes and the instrument reverts to its previous state. Press
[CANCEL DEFROST/FUMIGATE] to stop the defrosting process earlier if required.
Note
1 The compressor will not restart until 3 minutes after it stopped
2
Defrosting cannot take place if the Chamber or Cryobar temperature is above 0°C
3 If the Day 9 option is selected when setting the defrost times, then the instrument
will enter STANDBY (PSAVE) mode when defrosting finishes.
2.2.4.2 [IMMED FUMIGATE]
TAKE CARE WHEN FUMIGATION IS IN PROGRESS. BE AWARE OF THE HAZARDS POSED BY FORMALIN AND ACT ACCORDINGLY. REFER TO MATERIAL SAFETY DATA SHEET (MSDS) FOR FORMALIN
Note
1 The window MUST be shut and locked before fumigation can star t. If
[IMMED FUMIGATE] is pressed when the window is not shut, Err 4 is displayed and the fumigate cycle is aborted.
2 Fumigation can only be cancelled by [CANCEL DEFROST/FUMIGATE] during
stages iii and iv that follow. Stage v and vi cannot be cancelled.
Press [IMMED FUMIGATE] to initiate the following sequence:
i the [IMMED FUMIGATE] button LED lights and the display shows Ald
(Formaldehyde).
ii the window lock is activated if the window has not already been locked by manual
use of the [WINDOW LOCK] push-button.
iii a 5 minute defrost is started, then....
iv the compressor is switched off and a one hour chamber defrost starts,
v the Formalin trough is heated to 100°C for 45 minutes
vi the heater is switched off and fumigation takes place over four hours.
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vii the instrument reverts to its previous setting when the fumigate cycle is
finished.
Note
1 If the Day 9 option is selected when the fumigation time is set, then the instrument
enters Standby (PSAVE) mode when fumigation finishes.
2.2.4.3 [CANCEL DEFROST/FUMIGATE]
Press [CANCEL DEFROST/FUMIGATE] to terminate a Defrost or Fumigate sequence. The button LED remains lit until the process has finished.
2.2.4.4 [LAMP]
Press [LAMP] to switch on the fluorescent lamp to illuminate the work area. The button LED is lit when the light is on. The next press switches off the lamp and indicator.
2.2.4.5 [CRYOBAR BOOST]
Press [CRYOBAR BOOST] to turn on the peltier element in the Cryobar for 10 minutes. The compressor is also switched on for this period. (However, if the compressor
switched off within the last three minutes, there is a delay of up to three minutes before the compressor re-starts.)
If [CRYOBAR BOOST] is pressed while its LED is flashing at the end of the boost period, the boost is repeated for a further 10 minutes, otherwise normal operation follows when a boost period times out.
Note
1 The Cryobar is situated on the refrigeration pipework and its temperature can
vary considerably. Do not use the Cryobar for storing specimens.
2.2.4.6 [WINDOW LOCK]
[WINDOW LOCK] locks the window in the closed position. The window must be fully
closed before the lock can operate. A second press releases the lock. If the window is not properly closed when the button is pressed, Err 4 is displayed. The lock remains in the locked state during power off (if already locked).
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2.2.5 TEMPERATURE CONTROLS
To set the working temperature, the appropriate button must be pressed with [+] and [-] to raise or lower the value of the number in the display. Press and hold until the desired temperature is displayed, then release both buttons. The display then reverts to showing the actual temperature in °C.
Note
1 It is not possible to set the temperature of the Cryobar. The Cryobar is situated on
the refrigeration pipework and its temperature can vary considerably.
2 To monitor the temperature, press and release [CRYOBAR TEMP] or
[WORKING TEMP] as appropriate. The display will show the actual temperature of that component in °C. A lit LED shows which selection is displayed.
Working temperatures are automatically controlled to within 1°C of the set temperature. If no temperature is set, or after a reset, the working temperature automatically defaults to -20°C, or the last programmed temperature.
[CRYOBAR TEMP] displays the surface temperature of the Cryobar. However, if [CRYOBAR BOOST] is lit on the adjacent panel, the display shows the approximate
surface temperature of the peltier element in the Cryobar.
2.2.6 KEYSWITCH CONTROL
The Control Panel can be locked from the two-position keyswitch. The Control Panel is disabled when the key is vertical (off) and the LOCKED indicator is lit. The key is removable when vertical.
To unlock the Control Panel, insert the key and turn it clockwise. The LOCKED indicator is not lit when the Control Panel is unlocked, and the key is held in the switch.
CRYOBAR
TEMP
WORKING
TEMP
TEMPERATURE °C
+ -
LOCKED
R Y O T O M E
C
®
LOCKED
R Y O T O M E
C
®
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2.3 ADVANCE CONTROL PANEL
The Advance Control Panel is the horizontal panel located to the left of the window.
2.3.1 Standard Cryotome only
The Advance Control Panel on the standard Cryotome contains two rotary control knobs.
The small knob (a) is the Micron Selector. This sets the section thickness - the distance that the Specimen Head advances after each cutting stroke. The range is 0 - 30µm in increments of 1µm.
The larger knob (b) is the Rewind/Advance Control. Use this for coarse adjustment of the Specimen Head position and also to retract the Specimen Head to the Maximum Travel Available position.
Turn the Rewind/Advance Control knob clockwise to rewind the head and anti-clockwise to advance the head. One rotation of the knob equates to a movement of 100µm at the Specimen Head.
2.3.2 Cryotome E only
The Advance Control Panel on the Cryotome E contains the following buttons that control movement of the Specimen Head;
- REWIND
- ADVANCE
- FAST REWIND
- FAST ADVANCE
There is also a notched Electronic Rotary Control that advances or retracts the Specimen Head 8 microns per notch dependent on the direction of rotation - clockwise to advance; counter clockwise to retract.
b
a
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2.4 MESSAGE CODES
The following displays show in the Temperature display when conditions are appropriate.
CRYOBAR
TEMP
WORKING
TEMP
TEMPERATURE °C
+ -
i Ald shows when fumigation is in progress.
ii LOBAT signifies that the internal back-up battery is not charged sufficiently to
retain information during an interruption of the mains supply.
The battery retains programmed information in memory while the power is switched
off. Stored information is lost if it is allowed to discharge.
Power must be on to recharge the battery. If LOBAT still shows after power is
restored for more than 12 hours, contact your Thermo dealer for service.
iii dEF shows when defrosting is in progress.
iv --- shows if the associated temperature probe is faulty.
v Fault Codes show in the Temperature display if the instrument malfunctions. Press
[WORKING TEMP] or [CRYOBAR TEMP] to over-ride most faults. Faults F:11, F:12 and F:13, cause the instrument to wait for 30 mins then reset and attempt to reach its default temperature of -20°C.
Note
1 If the fault fails to clear, contact your Thermo Dealer for service.
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Indications that show in the Time Controls display are as follows:
i P FAIL (flashing) shows after a normal switch OFF then ON, or if power
failed and recovered. The Temperature display shows the temperature of the Refrigerated Chamber at the time power was restored (i.e. the warmest temperature).
Press [CLOCK] to clear.
ii rESEt (flashing) shows if a problem caused the instrument to reset and clear
its memory to its default status. The number in Temperature display is the temp eratur e of th e Re frigerated Chamber at the time normal operation resumed.
Press [CLOCK] to clear.
iii P SAVE shows until the programmed time if the instrument is in Standby
(PSAVE) mode following the selection of a DAY 9 Defrost or Fumigate function.
Press [CANCEL DEFROST/FUMIGATE] to override.
iv Err 1 denotes an invalid push-button entry, or if too many push-buttons are
pressed simultaneously.
Press [CLOCK] to clear.
v Err 2 denotes an invalid push-button entry during Defrost/Fumigate, or if Defrost
is inhibited because the Cryobar and Refrigerated Chamber are too warm
Press [CLOCK] to clear.
vi Err 4 denotes a window lock error (not locking or unlocking).
Press [CLOCK] to clear.
Note
1 Err 3 is not used on these versions of Cryotome.
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2.5 CONNECTIONS
The connections to the electronics box are shown below:
WINDOW BOOST
WINDOW
BOOST
a cb
a Mains On/Off Circuit Breaker
This is the main power switch of the instrument. Its use is fully described in section 3.5.
b Power Inlet
The mains power inlet. It is fully described in section 3.4.
c Window Boost
Press the Window Boost switch to provide extra de-misting power in conditions of high humidity. Its use is described in section 5.4.
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INSTALLATION AND SETTING UP
3.1 INTRODUCTION
The Cryotome is a precision instrument that must be unpacked and installed with care.
The maximum overall dimensions of the Cryotome cryostat are:
Width 660 mm (26 ins) Depth 640 mm (25¼ ins) Height 1070 mm (42¼ ins)
THE CRYOTOME WEIGHS APPROXIMATELY 120kg (265lbs). ALWAYS GET HELP TO SAFELY MOVE THE INSTRUMENT WITHOUT RISK OF INJURY
3.2 TO UNPACK
If the packaging has been damaged, check the condition of the instrument. Contact your dealer if there is any damage.
Check that the detail on the label of the crate corresponds with the Purchase Order, and that the power supply capability of your local socket outlet is compatible with the power demand of the Cryotome.
Move the crate near to where the instrument is to be sited. Cut the retaining straps then lift the outer case vertically. Read the label on the rear of the instrument and check that it conforms with your order.
Retrieve the accessories pack from on top of the pallet in front of the instrument, then lift the Cryotome off the Pallet. Make sure that you have received all the parts listed on the packing list supplied with the instrument. Contact your dealer if necessary
Notes
1 Inform your dealer immediately if there are any breakages or shortages. Quote
the instrument Serial Number, your Order Number, Invoice Number, Delivery Note (or Packing Slip) Number and the date.
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2 If you ever need to transport the instrument, refer to Appendix A for repacking
instructions.
Always keep the instrument vertical to prevent damage to the refrigeration system.
Consult your Thermo dealer if the Cryotome is to be moved to another building, or out of the area. Transit fixings must be re-fitted. The instrument must be kept upright at all times.
THE CRYOTOME WEIGHS APPROXIMATELY 120kg (265lbs). ALWAYS GET HELP TO SAFELY MOVE OR LIFT THE INSTRUMENT WITHOUT RISK OF INJURY
To move the Cryotome, turn the
lowering knobs clockwise until
the two front feet of the instrument are on the floor.
Push the instrument. Alternatively, two people can lift the instrument using the hand-holds on either side of the instrument.
THE INSTRUMENT IS HEAVY. ALWAYS MAKE SURE THE ROUTE IS CLEAR WHEN MOVING THE INSTRUMENT TO AVOID COLLISIONS
3.3 TO REMOVE THE TRANSIT FIXINGS
TRANSIT FIXINGS ARE ATTACHED TO VARIOUS INTERNAL STRUCTURES TO PROTECT THE INSTRUMENT DURING TRANSPORT. IT IS ESSENTIAL THAT THESE ARE ALL REMOVED BEFORE AN ATTEMPT IS MADE TO OPERATE THE INSTRUMENT
Note
1 The transit fixings that are to be removed are painted red.
2 Store all the transit fixings for future use when transporting the Cryotome.
WARNING
WARNING
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The rear panel must be removed to reach the transit fixings.
To remove the rear panel, undo and remove the bolt from the centre of the rear panel. Undo the 8 screws that secure the rear panel, then carefully pivot the panel around its left hand side (as seen from the rear of the instrument). Lean the panel against the left hand side panel.
DO NOT DISTURB THE EARTH WIRE THAT IS CONNECTED TO THE REAR PANEL
Refrigeration Transit Fixings:
Undo and remove the two red handles (a) from the centre of the compressor mounting plate between the fan (b) and the compressor (c).
Remove the two wood supports (d), coloured red, from under the compressor mounting plate.
Make sure that the power lead from the compressor to the junction box (e) is securely fitted.
Firmly hold the blue plastic coil of the defrost valve on the transit plate at the back of the microtome, then cut the ties that hold the valve (f).
With the coil of the defrost valve still connected to the white wires, place the coil over the tube that projects upward from the copper tube on top of the compressor cooling matrix (g). Unpack the blue plastic components and fit the nameplate, the washer, and then the nut to hold the coil of the defrost valve in position. Fit the plastic plug in the hole in the centre of the rear panel.
f
g
e
d
a
b
c
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j
k
Microtome Transit Fixings: [E only]
From the underside, support the stud of the clamping bracket that prevents damage t o the microtome leadscrew, then undo and remove the top nut (h). Support the stud from underneath while allowing it to fall clear of the leadscrew arm.
Support the leadscrew arm and use the nylon loop (i) to remove the spacer from between the arm of the leadscrew and the casting of the microtome.
Check that the detection flag at the end of the arm is in line with the slots in the two black plastic opto units of the printed circuit board (j). This signifies that the leadscrew and trunnion nut are located correctly.
Note
1 Turn the leadscrew to adjust the setting if
necessary.
Undo and remove the transit plate that supported the defrost valve (k). Replace the rear panel and 8 screws. Retain the transit plate for future use when moving the Cryotome.
3.4 TO INSTALL THE CRYOTOME
Move the instrument to its permanent location. This must be level and provide a 300 mm (12”) clearance to the sides and rear of the instrument for ventilation.
300mm
h
i
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