Thermo Scientific CryoStar NX70 series, CryoStar NX70 HOMPD, CryoStar NX70 HOMP, CryoStar NX70 HOMPV, CryoStar NX70 HOMPDV Operator's Manual
Thermo Scientific CryoStar NX70
Product Code
956960, 956970, 956980, 956990, 957000, 957010,
957020, 957030, 957040, 957050, 957060, 957070
Operator Guide
387928 Issue 10
Thermo Scientific CryoStar NX70 Operator Guide
2
Thermo Scientific CryoStar NX70 Operator Guide
3
Company Information
© Copyright 2016. Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. (Thermo Fisher Scientific) is the world leader in serving science, offering a
unique combination of innovative technologies. Thermo Scientific is a brand name of Thermo Fisher
Scientific.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting
document is correct and clearly stated, but does not accept responsibility for any errors or omissions. The
development of Thermo Scientific products and services is an ongoing process. Please ensure that any
published information you use as a reference is up to date and relates to the condition of the product. If
necessary, check with your local Thermo Fisher Scientific representative.
This document may not, in whole or in part, be copied, photocopied, reproduced, translated, or
converted to any electronic or other form without prior written consent of Thermo Fisher Scientific. All
information contained in this manual is proprietary and confidential, and the exclusive property of
Thermo Fisher Scientific and is protected by copyright.
Contact Addresses:
Thermo Shandon Limited (Trading as Thermo Fisher Scientific),
Tudor Road, Manor Park,
Runcorn, WA7 1TA, UK
Tel: +44 (0) 1928 534 000; Fax: +44 (0) 1928 534 001
Email: sales.ap.uk@thermofisher.com
Web: http://www.thermoscientific.com/pathology
USA Distributor:
Richard-Allan Scientific
Subsidiary of Thermo Fisher Scientific
4481 Campus drive
Kalamazoo
MI 49008
USA
This instrument conforms to the essential requirements of:
IVD Directive 98/79/EC
and its harmonized standards under Union harmonization legislation
Thermo Scientific CryoStar NX70 Operator Guide
4
NX70 110V
EC Regulation No 517/2014 – Fluorinated Greenhouse Gas Regulations
Conta ins fluorina ted greenhouse ga ses
Refrigerant: R404A
R404A GWP value = 3992
System charge weight = 0.400 Kg
System CO2 equiva lent charge weight = 1.597 tonnes
NX70 230V
EC Regulation No 517/2014 – Fluorinated Greenhouse Gas Regulations
Conta i ns fluorina ted greenhouse ga ses
Refrigerant: R404A
R404A GWP value = 3992
System charge weight = 0.400 Kg
System CO2 equiva lent charge weight = 1.597 tonnes
EMC Statement
This IVD equipment complies with the emissions and immunity requirements of IEC 61326-2-6:2012
and IEC 61326-1:2012.
This equipment has been designed and tested to CISPR 11:2009+A1:2010 Class A.
It is intended for use in a laboratory environment by a trained and qualified professional. In a domestic
environment it may cause radio interference, in which case it may be necessary to take measures to
mitigate the interference.
Thermo Scientific CryoStar NX70 Operator Guide
5
Important Note Regarding Instrument Variants
This document is designed for use with all variants of the Thermo Scientific CryoStar NX70 cryostat.
A range of optional features are available on this instrument and the instructions contained in this manual
are not specific to any one variant. Thus some features described may not be available on your instrument.
This IFU is valid for the variants listed below:
Product Code Product Variant
956960 CryoStar NX70 HOMP 100V 60 Hz
956970 CryoStar NX70 HOMPD 100V 60 Hz
956980 CryoStar NX70 HOMPV 100V 60 Hz
956990 CryoStar NX70 HOMPDV 100V 60 Hz
957000 CryoStar NX70 HOMP 110-120V 60 Hz
957010 CryoStar NX70 HOMPD 110-120V 60 Hz
957020 CryoStar NX70 HOMPV 110-120V 60 Hz
957030 CryoStar NX70 HOMPDV 110-120V 60 Hz
957040 CryoStar NX70 HOMP 220-230V 50/60Hz
957050 CryoStar NX70 HOMPD 220-230V 50/60Hz
957060 CryoStar NX70 HOMPV 220-230V 50/60Hz
957070 CryoStar NX70 HOMPDV 220-230V 50/60Hz
Thermo Scientific CryoStar NX70 Operator Guide
6
Symbols
The following symbols and conventions may be used throughout this document and on the instrument:
This symbol is used on the instrument, or in a document, to indicate that instructions
must be followed for safe and correct operation. If this symbol appears on the
instrument, always refer to the operator guide.
This symbol is used on the instrument, or in a document, to indicate that there are
potential biological risks associated with the instrument and / or instrument use. Always
use Good Laboratory Practice.
Cold surface, if necessary, use gloves
Cutting hazard, sharp edges, watch your fingers.
This symbol is used on the instrument, or in a document, to indicate that irritants or
potentially harmful chemicals are present. Refer to the Material Safety Data Sheets for
the products, and always use Good Laboratory Practice.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European
countries with a separate collecting system within the waste management. This product,
being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).
Manufacturer.
Serial number
It is stated on the product label sticker.
It is built up as follows: SYYMMXXXX
S=Production site, Y=year of production, M=Month of production, X= Counter
So the serial number states the production date of the device
A warning is given in the documentation if there is a danger of personal injury or damage to the
equipment or samples.
Note
Notes give additional information about a job or instruction, but do not form part of the instruction.
SN
Thermo Scientific CryoStar NX70 Operator Guide
7
Table of Contents
COMPANY INF ORMATION............................................................................................................................................ 3
EMC STATEMENT .......................................................................................................................................................... 4
IMPO RTANT NOTE REGARDING INSTRUMENT VARIANTS ....................................................................................... 5
SY MB OL S ........................................................................................................................................................................ 6
TABL E OF CON TENTS..................................................................................................................................................... 7
CHAPTER 1 - SAFETY INFORMA TION ......................................................................................................................... 10
GENERAL SAFETY.......................................................................................................................................................... 10
CHEM ICAL SA FET Y ........................................................................................................................................................ 11
ENVI RONMENT............................................................................................................................................................. 11
WARRANTY STATEMENT................................................................................................................................................ 12
CHAPTER 2 - INTRO DUCTION ..................................................................................................................................... 13
INT ENDE D USE ............................................................................................................................................................. 13
INS TRUME NT FEATURES ................................................................................................................................................ 13
TECHNICAL SPECIFICATIONS ........................................................................................................................................... 14
Mechanical Specifications.................................................................................................................................. 14
Electrical Specifications ...................................................................................................................................... 14
Environmental Specifications............................................................................................................................. 14
Cooling Specifications......................................................................................................................................... 15
Microtome and Sectioning Specifications ......................................................................................................... 15
Cold D Specification ............................................................................................................................................ 16
Default Settings .................................................................................................................................................. 16
CHAPTER 3 - INSTRUMENT SETUP ............................................................................................................................. 18
UNPACKING A ND REPACKI NG ......................................................................................................................................... 18
Safety Precautions .............................................................................................................................................. 18
Unpacking Procedure ......................................................................................................................................... 18
Repacking the Instrument .................................................................................................................................. 19
LOCAT ION & SET UP ...................................................................................................................................................... 20
IDENTI FICA TION O F PA RTS ............................................................................................................................................. 21
Overview.............................................................................................................................................................. 21
Connections Panel .............................................................................................................................................. 22
Cryochamber ....................................................................................................................................................... 23
Blade Holder........................................................................................................................................................ 24
Specimen Head NX70 ......................................................................................................................................... 25
SET UP........................................................................................................................................................................ 26
Fitting or Exchanging the Anti Roll Plate .......................................................................................................... 26
Fitting or Exchanging Low Profile Blades .......................................................................................................... 27
Removing Used Blades with the Magnetic Blade Tool..................................................................................... 28
Blade Positioning and Orientation .................................................................................................................... 29
Adjusting the Cutting Angle ............................................................................................................................... 30
Adjusting the Anti-Roll P la te .............................................................................................................................. 32
SPECIMEN SETUP.......................................................................................................................................................... 33
Specimen Orientation ......................................................................................................................................... 33
FILLING WITH DISINFECTANT TO PERFO RM THE COLD D FUNCTION ..................................................................................... 35
Thermo Scientific CryoStar NX70 Operator Guide
8
Filling the Cryostat with Disinfectant Solution (Sanosil®) ................................................................................ 35
CHAPTER 4 – OP ERA TION ........................................................................................................................................... 37
SWITCHING ON ............................................................................................................................................................ 37
SYSTEM INTE RFA CING ................................................................................................................................................... 38
Using the Touchscreen ....................................................................................................................................... 38
Main Screen ........................................................................................................................................................ 38
SETTING PREFERENCES .................................................................................................................................................. 40
Quick Freezing of the Specimen......................................................................................................................... 40
Trimming and Fine Section Thickness................................................................................................................ 41
Temperature ....................................................................................................................................................... 43
Set the Blade Temperature ................................................................................................................................ 44
Cutting Window .................................................................................................................................................. 45
Select a Favourite ............................................................................................................................................... 46
Vacutome and Cleaning Mode .......................................................................................................................... 47
Height Adjustment.............................................................................................................................................. 51
Multi-Fun ction Bar.............................................................................................................................................. 52
Time and Date..................................................................................................................................................... 52
Cutting Mode Selection ...................................................................................................................................... 53
Handwheel Brake................................................................................................................................................ 58
Multi-Function B utton ........................................................................................................................................ 59
Menu ................................................................................................................................................................... 61
Chamber Light..................................................................................................................................................... 62
Stand by................................................................................................................................................................ 63
Display Lock......................................................................................................................................................... 64
Favourites............................................................................................................................................................ 65
User Guide........................................................................................................................................................... 67
Settings Menu ..................................................................................................................................................... 69
Language............................................................................................................................................................. 70
Time and Date..................................................................................................................................................... 71
Start Time ............................................................................................................................................................ 74
Defrost ................................................................................................................................................................. 76
Immediate Defrosting ........................................................................................................................................ 76
Programming the Daily Defrost Cycle ............................................................................................................... 78
Screen .................................................................................................................................................................. 80
Retraction............................................................................................................................................................ 81
Data Log .............................................................................................................................................................. 83
Default Settings .................................................................................................................................................. 84
Serv ice ................................................................................................................................................................. 85
EMERGENCY STOP ........................................................................................................................................................ 86
Standard Emergency Stop Function .................................................................................................................. 86
Optional Foot Pedal Emergency Stop................................................................................................................ 87
COOLING PROCESSES .................................................................................................................................................... 87
Specimen and blade holder cooling................................................................................................................... 87
SECT IONI NG PRO CEDURES ............................................................................................................................................. 88
Cutting Movement and Retraction.................................................................................................................... 88
Trimming and First Cuts ..................................................................................................................................... 89
Trimming ............................................................................................................................................................. 89
Sectioning and Taking Off Sections ................................................................................................................... 90
Thermo Scientific CryoStar NX70 Operator Guide
9
HANDWHEEL BRAKES .................................................................................................................................................... 91
Electronic Handwheel Brake .............................................................................................................................. 91
Mechanical Handwheel Brake ........................................................................................................................... 92
COLD D....................................................................................................................................................................... 93
Starting the Disinfection (Cold D) Function....................................................................................................... 93
Abo rting th e Disinfection ................................................................................................................................... 95
Programming Cold D .......................................................................................................................................... 96
CHAPTER 5 - TROUBLESHOOTING.............................................................................................................................. 98
TROUBLESHOOTING TABLES ........................................................................................................................................... 98
TIPS FOR SUCCESSFUL SECTIO NING ...............................................................................................................................100
How to avoid malfunctions when working with the Vacutome ....................................................................101
Sectioning Temperature Guidelines fo r Fresh Ti ssu es....................................................................................102
CHAPTER 6 - CLEAN ING AND MAINTENANCE.........................................................................................................103
CLEANI NG THE TO UCH SCREEN DISPLAY ........................................................................................................................103
SHUTTING OFF FOR CLEANI NG .....................................................................................................................................103
CLEANI NG AND CARE O F THE BLADE HOLDER ..................................................................................................................103
EMPTYING THE DEF ROSTI NG AND COLD D LIQUIDS .........................................................................................................104
EXCHANGE THE VACUT OM E FILT ER BAG ........................................................................................................................105
ADJUSTMENT OF THE MOV ABLE SPECIMEN HEAD JAW ....................................................................................................107
TRANSPORTATION OF THE INSTRUM ENT.........................................................................................................................108
Conditions for the Transportation of the Instrument.....................................................................................108
APPEN DICES ...............................................................................................................................................................110
APPENDIX A - SPARES AND ACCE SS ORI ES .......................................................................................................................110
APPENDIX B - RECOMMENDED DIS INFECTIO N PROCE DURE ..............................................................................................112
APPENDIX C - COLD DISINFECTION CERTIFICATE .............................................................................................................113
INDEX ..........................................................................................................................................................................114
Thermo Scientific CryoStar NX70 Operator Guide
10
Chapter 1 - Safety Information
Thermo Scientific instruments are designed for convenient and reliable service; however, improper use or
handling by a user may damage the instrument. Protection might be impaired if the equipment is used in
a manner not specified by the manufacturer. Correct maintenance procedures are essential for consistent
performance. It is recommended that users secure a maintenance contract with our service department
acting on behalf of Thermo Shandon Limited.
Any problems and queries should be referred to your Thermo Fisher Scientific service department.
The following sections contain important information for the safe setup and use of the
instrument, and should be read and understood by the user before using the instrument.
General Safety
This instrument, as supplied, conforms to IEC 61010-1 and IEC 61010-2-
101; however, the
addition of chemicals introduces potential hazards. Good Laboratory Practice must be
employed and consideration must be given to the potential for hazard when dealing with
these chemicals.
Do not use the instrument in close proximity to strong electromagnetic radiation, as these
may interfere with the proper operation. The electromagnetic environment should be
evaluated prior to operation of the device.
Good Laboratory Practice must be used when handling tissue samples to prevent cross
contamination and infection. The user should complete a risk assessment to determine any
potential hazards related to tissue handling.
Do not introduce any source of ignition into, or near, the instrument once it has been loaded
with reagents.
Do not remove any panels or access covers, unless specifically instructed to do so. The
instrument does not have any user serviceable parts. Potentially lethal voltages are present
inside the instrument.
The instrument must be properly connected to a good earth (ground) via the Mains input
supply and positioned such that it is possible to interrupt the Mains supply at the source by
removing the plug from the socket.
Use only factory approved accessories or replacement parts within the instrument.
Only use reagents recommended in the operator guide.
Thermo Scientific CryoStar NX70 Operator Guide
11
Chemical Safety
The introduction of chemicals creates potential hazards. Thermo Fisher Scientific has adopted the
following position with regard to the subject of volatile chemicals used in laboratories:
• Customers using non-specified chemicals in the instrument, do so at their own
risk.
• All chemicals recommended by Thermo Fisher Scientific have auto-ignition
temperatures considerably above any surface temperatures that can be reached
during a single fault failure on the instrument.
• The instrument contains no source o
f ignition in any areas of the instrument where
chemicals are stored, or likely to leak into, in a single fault condition.
•
The operator is fully aware of the contents of the specification documents detailing
the properties of the chemicals they are using.
• The operator has carried out any legally required assessment of chemicals used and
is using Good Laboratory Practice.
Environment
This instrument complies with the European Union's Waste Electrical and Electronic Equipment
(WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific on behalf of Thermo Shandon Limited has contracts with one or more recycling
/ disposal companies in each EU Member State, and this product and packaging should be disposed of or
recycled through them. For further information contact your Thermo Fisher Scientific service
representative.
Thermo Scientific CryoStar NX70 Operator Guide
12
Warranty Statement
Thermo Shandon Limited as part of Thermo Fisher Scientific is proud of their quality, reliability and of
our after-sales service. We continuously strive to improve our service to our customers.
Please ask your distributor or Thermo Fisher Scientific representative about service contracts which can
help maintain your instrument in an optimal operating condition.
Warranty provisions necessarily vary to comply with differences in national and regional legislation.
Specific details can be found in the delivery documentation or from your dealer or representative.
Please note that your warranty may be invalidated if:
• This instrument is modified in any way, or not used as intended by The r mo Shandon L i mited.
• Accessories and reagents which have not been approved by Thermo Fisher Scientific are used.
• The instrument is not operated or maintained in accordance with instructions.
Thermo Scientific CryoStar NX70 Operator Guide
13
Chapter 2 - Introduction
Intended Use
The Thermo Scientific cryostat NX70 is an in vitro diagnostic device, designed to take
precision sections of tissue specimens including the sectioning of paraffin embedded
samples in medical, pharmaceutical laboratories as necessary preparation for their
examination. Only qualified and trained laboratory personnel may operate the NX70.
The instrument may only be operated within the scope of its intended use as described above and as per
the instructions given in this manual.
Any other use of this instrument is considered as improper operation.
Instrument Features
The CryoStar NX70 is a precision-engineered, ergonomically-designed, high-performance cryostat in
modular design with a stainless steel cooling chamber. Some of its features are:
• Knife / Blade Holder cooling.
• Specimen cooling.
• Ergonomic height adjustment with integrated ergonomic casing.
• Colour touch-screen user interface and joystick control.
• Motorized cutting with variable speed control (from 0 to 256 mm/s).
• Optional Cold D function consisting of an internal fumigation unit that applies Sanosil®
(disinfection medium) into the user-accessible, operating areas of the cryochamber.
• Optional Vacutome function.
Thermo Scientific CryoStar NX70 Operator Guide
14
Technical Specifications
Mechanical Specifications
Depth 100 cm (39.4 in)
Width 75.5 cm (29.7 in)
Height Range Adjustable range 82 - 112 cm (32.3 - 44.0 in)
Maximum Weight 200 kg (440 lb)
Electrical Specifications
Power Requirements
Dedicated line recommended.
100V, 50/60Hz, 10A, +/-10%, 110-120V, 60Hz, 10A, +/-10%
220-230V, 50/60Hz, 5A, +/-10%
Environmental Specifications
For Indoor Use Only
Temperature (Operating
Limits)
+5°C to +35°C (+41°F to +95°F)
Temperature
(Recommended
Operation)
+15°C to +30°C (+59°F to +86°F)
Note:
Performance may deteriorate when operated outside of this
range.
Temperature
(Transport and Storage)
-20°C to +40°C (-4°F to 122°F)
+70°C (158°F) for short exposure
Relative Humidity Max. 80% RH up to 31°C
Decreasing linearly to 50% RH at 40°C
Altitude Up to 2000 m (6,500 ft)
Pollution Degre e 2
Over Voltage Category II
Floor Loading
Requirements
300 kg/m² (660 lb/ft²)
Protection Class I
Sound Emission <45 dB (A) - measured with 1m distance to the instrument.
Refrigerant R404a, filling amount 400g
Thermo Scientific CryoStar NX70 Operator Guide
15
Cooling Specifications
Specimen Temperature
Control
-50°C to +10°C (-58°F to +50°F) at +20°C room Temp. (decrease
0,7°C/1°C room temp. increase)
Blade Temperature
Control
-35°C to -5°C (-31°F to +23°F) at 20°C room Temp (decrease 0.5°C
71°C room temp. increase)
Cryobar Cooling Max. -60°C (-76°F) at -35°C blade temperature
Defrosting Options Programmed / Immediate
Microtome and Sectioning Specifications
Section Thickness Range 0.5 – 500 µm
Fine Section Thickness
Range
0.5 - 100 µm
Fine Section Thickness
Resolution
0.5 – 2 µm in 0.5 µm steps
2 µm – 10 µm in 1 µm steps
10 µm – 20 µm in 2 µm steps
20 µm – 50 µm in 5 µm steps
50 µm – 100 µm in 10 µm steps
Trimming Thickness
Range
5 - 500 µm
Trimming Thickness
Resolution
5 – 10 µm in 5 µm steps
10 µm – 100 µm in 10 µm steps
100 µm – 200 µm in 20 µm steps
200 µm – 500 µm in 50 µm steps
Specimen Retraction
(Return Travel)
20 µm
Vertical Specimen Stroke 64 mm
Horizontal Specimen
Movement
48 mm
Specimen Approach Manual (variable speed by joystick control), maximum speed: 3mm/s
Chuck Size 30, 40 mm (additional special sizes are available)
Specimen Orientation x - and y - axes universal 7° with zero positioning; z - axis up to 360°
Thermo Scientific CryoStar NX70 Operator Guide
16
Coarse Feed Motorized
Cutting Drive Motorized, electronically controlled and manual via handwheel
Cutting Window Manually adjusted to specimen size
Operating Modes Interval, single, multi- and continuous-stroke
Cutting Speed 0 - 256 mm/s (0-120 1/min)
Clearance Angle
Adjustment
Disposable blade carrier 8- 16°
Vacutome Filter approximately 500 ml
Cold D Specification
Reservoir Volume 1000 ml
Recommended Medium Sanosil® S010 (5% H2O2)
Default Settings
Counter Mode Speed V = 0
Multi Counter 2
Operating Mode Continuous
Retraction ON
Language English
Fine 0.5 µm
Trim 5 µm
Joystick Calibration Calibrated
Chamber Light 100%
Screen Brightness 100%
Brake ON
Stepper Motor Initialization Yes
Blade Temperature -15°C
Specimen Temperature -15°C
Cutting Window 0 mm (OFF)
Favorites (Program 1, Program 2,
Program 3)
(-15°C/-15°C), (-20°C/-20°C), (-25°C/-25°C)
Cryobar OFF
Vacutome OFF
Disinfection OFF
Thermo Scientific CryoStar NX70 Operator Guide
17
Disinfection, Cycle (Automatic) ON (03:00 Night)
Defrost Cycle (Automatic) ON (00:00 Night)
Standby Mode 1h (After 1 hour, the Cryostat enters the Standby Mode
automatically). Kept temperature: -15°C.
Sleep Mode 3h (After 3 hours, the Cryostat enters the Sleep Mode
automatically). Kept temperature: -10°C. Power savings:
Approximately 30%.
Start Time 06:00 a.m.
Section Counter 0
Section Sum 0
Thermo Scientific CryoStar NX70 Operator Guide
18
Chapter 3 - Instrument Setup
Unpacking and Repacking
Safety Precautions
The CryoStar NX70 is heavy and requires a minimum of two people to safely unpack and
manoeuvre it.
The CryoStar NX70 has a high centre of gravity and care should be taken when moving.
Do not tip the CryoStar NX70more than 30° from the upright position.
After moving the CryoStar NX70, wait at least 8 hours before switching on to allow the
refrigerant to settle - failure to do so may cause damage to the unit.
Note
The packing materials should be carefully stored for use in any future instrument shipment.
Unpacking Procedure
General Information:
• Ensure that these Instructions are retained for future use.
• The CryoStar NX70 is delivered on a pallet. The sides of the instrument are encased in cardboard
and cushioned with foam spacers inside the container. A wooden cover protects the top of the
instrument.
• In unpacking be aware of the weight (200 Kg/440 lbs) of the instrument.
Safe handling of the installation requires the involvement of two people
Handling
:
• Place the crate on a flat surface while ensuring that there are no objects underneath the pallet.
• Remove the wooden cover form the crate but retain it for later use as a ramp.
• Remove the top foam spacer from the crate.
• Remove the exterior cardboard container by opening the clips.
• Remove the remaining foam spacers and the accessories carton. Place all the accessories carefully
to one side.
• Remove the plastic cover from the instrument.
• Two wing-nuts are now visible at the front edges of the wooden pallet.
Thermo Scientific CryoStar NX70 Operator Guide
19
• Using the wing-nuts affix the wooden top of the crate to the pallet to serve as an unloading ramp
for the instrument.
• The instrument can be moved on its castors. However, ensure that it does not roll off the pallet
inadvertently.
• Screw in the two bails (part of the standard accessories) into the respective holes in the foot of the
cryostat.
• Mount the transport belts (part of the standard accessories) with the bails.
To move the instrument from the pallet stand BEHIND (NOT in front) of the
instrument.
• Carefully roll the instrument off the pallet and on to the floor.
• Move the instrument to the desired location while leaving space on the left- and right-hand.
• Keep all packing and transportation materials together in case of future need.
• In the future, if the instrument is transported by forklift, truck, train, ship or aircraft it must be
packed in the original shipping container with all transportation locks in place.
Repacking the Instrument
• To repack the instrument, follow the above instructions in reverse order.
Thermo Scientific CryoStar NX70 Operator Guide
20
Location & Setup
When deciding where to locate the CryoStar NX70, the following items must be taken into
consideration:
• Approximately 10 cm (4 in) must be allowed around the cooling system to allow free air flow.
• Approximately 10 cm (4 in) must be allowed between the wall and the rear panel.
• The vents on the sides of the rear panel must be unobstructed at all times.
• The mains power socket must be accessible at all times.
• The location must be free from draughts, open doors or air conditioning systems.
• The location must not expose the cryochamber to direct sunlight.
• Enough room for height adjustment.
Note
These measures are intended to reduce the build-up of frost and maintain consistent cooling temperatures
resulting in more favourable working conditions. Locations which have high humidity and / or high
ambient temperatures can reduce the performance of the instrument.
Once the CryoStar NX70 has been located, ensure the fixing bolts shown are tightened to
prevent the unit from moving during operation.
Thermo Scientific CryoStar NX70 Operator Guide
21
Identification of Parts
Overview
1
Cryochamber
6
Connections Panel
2
Cryochamber Window
7
Handwheel
3
Touch Screen
8
Handwheel Mechanical Brake
4
Cold D Reservoir Cover
9
Water Drain Reservoir Cover
5
Emergency Stop Button
Thermo Scientific CryoStar NX70 Operator Guide
22
Connections Panel
10 Power Switch 13 X1 – RJ45 port (for Service purposes only)
11 Power Socket 14 X2 – USB port (for service purposes only)
12 Rating and Serial Number Label 15 X3 – Foot Pedal Connector
Thermo Scientific CryoStar NX70 Operator Guide
23
Cryochamber
16 Cryobar and Cooled Storage Area 23
Cutting Speed Control
17 Trim Button 24
Motorized Cutting
Start / Stop Button
18 Joystick 25
Handwheel Electronic Brake Button
19 Specimen Head 26
Vacutome Filter Cover
20 Blade Holder with Anti-Roll Plate 27
Vacutome Filter Cover Release Knob
21 Vacutome 28
Section Waste Tray
22 Mode Selection Button
Thermo Scientific CryoStar NX70 Operator Guide
24
Blade Holder
29
Lateral Adjustment Lever
33
Blade Holder Base
30
Anti
-Roll Plate
34
Anti-Roll Plate Adjustment
31
Blade Clamping Lever
35
Clearance Angle Adjusting Screw
32
Top Stage (Angle Adjustment)
Thermo Scientific CryoStar NX70 Operator Guide
25
Specimen Head NX70
36 Y-axis Fine Adjustment Knob 39 Specimen Head Clamping Lever
37 Specimen Chuck Release Lever 40 Upper Specimen Chuck Jaw (Static)
38 X-Axis Fine Adjustment Knob 41 Lower Specimen Chuck Jaw (Moving)
Thermo Scientific CryoStar NX70 Operator Guide
26
Set Up
Before sectioning, the blade holder and the Cryochamber should be at a stable temperature close to the
desired cutting temperature. These temperatures should be selected to suit the consistency of the
specimen.
All tools which come into contact with the sections or which are used to manipulate the specimen must
also be cooled to prevent the tissue from sticking to them. Therefore all accessories should be stored on
the shelves or in the brush tray in the Cryochamber. The exceptions to this rule are the specimen chucks
which should be kept at room temperature.
The heated sliding window should be closed during periods of inactivity and while preparing specimens
to avoid warm air getting into the chamber. This will result in more favourable working conditions and a
lowe r frost build-up.
Ensure that the Vacutome filter is properly inserted in the filter unit and is suitably maintained - see
Cleaning and Maintenance.
Fitting or Exchanging the Anti Roll Plate
• The Anti Roll Plate is held in place by two black screws.
• Ensure that the gap clearance side of the Anti-Roll Plate is facing inwards.
• Position the Anti Roll Plate in the
holder, gently pushing the glass to the
bottom of the holder.
• Tighten the black screws.
Note
There are two usable edges on the Anti Roll Plate.
Thermo Scientific CryoStar NX70 Operator Guide
27
Fitting or Exchanging Low Profile Blades
Microtome blades are extremely sharp and can cause severe injuries- always use good
laboratory practice when handling them.
Always cover the blade with the knife / blade guard and activate the electronic and
mechanical brakes prior to making modifications to the blade/knife and specimen or
changing the specimen.
The blade holder should be fitted with low profile blades (80mm x 8mm; 35° facet angle) as standard.
To fit or exchange low profile blades:
• Use only the front handle to
move the anti-roll plate to the
side.
• Pull the blade clamping lever
towards the front of the
instrument to release the
clamping plate.
• Carefully slide a low profile blade
into the middle of the slot,
ensuring that the blade is
equidistant from the ends of the
slot.
• Push the blade clamping lever
towards the back of the
instrument to clamp the blade in
position.
Thermo Scientific CryoStar NX70 Operator Guide
28
Removing Used Blades with the Magnetic Blade Tool
The magnetic blade tool allows the user to remove used blades from the blade holder in a safe way.
Remove the used blade with the magnetic blade tool:
• Use only the front handle to
move the anti-roll plate to the
side.
• Pull the blade clamping lever
towards the front of the
instrument to release the
clamping plate.
• Move the magnetic blade tool
over the blade.
• Carefully lift the blade out of the blade holder and dispose of it in accordance with laboratory
regulations.
Thermo Scientific CryoStar NX70 Operator Guide
29
Blade Positioning and Orientation
Always cover the blade with the knife/blade guard and activate the electronic and
mechanical brakes prior to making modifications to the blade/knife!
If the cutting area of the blade is no longer usable, the upper part of the blade holder can be moved
without removing the blade.
To move the upper part of the blade holder:
• Loosen the lateral adjustment lever.
• The upper part of the blade holder should now be free to move left and right.
• Move it either to the left or to the
right until the desired area of the
blade is directly below the specimen
head.
• Move the lateral adjustment lever
forward to secure the upper part of
the blade holder in the new
position.
Thermo Scientific CryoStar NX70 Operator Guide
30
Adjusting the Cutting Angle
The cutting angle can be adjusted. The recommended cutting angle is approximately 10° for Thermo
Scientific low profile blades.
Note
The factory pre-set cutting angle is 10°.
• Loosen the clearance angle
adjustment screw on the lower left
site of the blade holder.
• Gently push both sides of the blade
holder base to select the desired
angle.
The surface of the blade holder base might be cold. Use gloves if necessary
Note
The clearance angle can be read on the scale on the left side on the upper part of the blade holder.
Note
10° is indicated by the longer increment in the middle of the scale.
Thermo Scientific CryoStar NX70 Operator Guide
31
• Re-tighten the clearance angle
adjusting screw to fix the selected
clearance angle.
Thermo Scientific CryoStar NX70 Operator Guide
32
Adjusting the Anti-Roll Plate
The fine adjustment of the anti-roll plate is
carried out using the knurled screw.
• To raise the height of the Anti-Roll Plate, turn the screw anti clockwise.
• To lower the height of the Anti-Roll Plate, turn the screw clockwise.
Note
Always adjust the height of the Anti-Roll Plate in small increments.
Thermo Scientific CryoStar NX70 Operator Guide
33
Specimen Setup
Specimen Orientation
Before sectioning can begin, the specimen must be correctly clamped and oriented in the specimen head.
To clamp and orient the specimen:
• Loosen the specimen chuck release lever
and insert the specimen chuck into the
jaws of the specimen head.
• Release the specimen chuck release lever again to allow the jaws to grip the chuck.
• Loosen the specimen head clamping lever
to allow adjustment of the specimen
parallel to the cutting edge.
• Use the fine adjusting knobs to orient the
specimen on the x- and y-axes.
Thermo Scientific CryoStar NX70 Operator Guide
34
Note
The fine adjustment knobs will click into place when the centre position has been reached.
• Tighten the specimen head clamping lever
again.
For further specimen orientation, the specimen chuck can also be rotated by 360° as described below:
• Loosen the specimen head clamping lever slightly.
• Rotate the chuck as required.
• Move the specimen head clamping lever back to its original position.
• After the specimen is adjusted, set the cutting window limits - see Setting the Cutting Window.
• Use the motorized coarse feed to position the blade close to the specimen.
• Tilt the joystick forwards to move the blade holder towards the specimen.
Thermo Scientific CryoStar NX70 Operator Guide
35
Filling with Disinfectant to Perform the Cold D Function
The Cold D function is an integrated system for the delivery of disinfectant (Sanosil®) into the cold
cryostat chamber. This method of disinfection ensures an even distribution of the disinfectant throughout
the Cryochamber.
The Cold D cycle takes 50 minutes to complete and the instrument cannot be used during this time;
however, as soon as the Cold D cycle is complete, the cryostat can be used safely.
To avoid frosting of the chamber, Cold D should not be used more than 2 times per day
plus one cycle overnight.
Filling the Cryostat with Disinfectant Solution (Sanosil®)
To load the cryostat with disinfectant:
• Open the Cold D reservoir cover to access
the filling inlet of the disinfectant tank.
Before filling the tank, check the shelf life and the hazardous substance warnings on the
disinfectant.
Note
Always follow the manufacturer’s instructions for handling, storage and disposal of the disinfectant as well as the
information provided by the manufacturer concerning any precautionary measures to be taken in the event of an
accidental release or spillage of the disinfectant.
• Place the funnel into the inlet.
Thermo Scientific CryoStar NX70 Operator Guide
36
• Empty the contents of one litre Sanosil into the
tank.
• Close the reservoir cover.
Thermo Scientific CryoStar NX70 Operator Guide
37
Chapter 4 – Operation
Switching On
Before switching on the instrument for the first time, ensure that the power requirements
indicated on the rating label correspond to the power supply voltage being used.
Note
No other instruments should be connected to the circuit used for the cryostat, as the compressor needs high
surge currents when started.
Do not use multi-socket power outlets with small wire sizes for the supply of the instrument.
• Insert the supplied power cord into the power socket and then connect the power cord to the
mains supply.
• Install the blade holder if necessary.
• Close the heated window.
• Turn on the power switch.
Note
The default setting of the blade holder and specimen head temperature are -15°C.
The cooling phase will take approximately 2 to 3.5 hours depending on the set chamber temperature.
Thermo Scientific CryoStar NX70 Operator Guide
38
System Interfacing
Using the Touchscreen
The CryoStar NX70 has an embedded touchscreen user interface which is used to set all preferences.
Avoid using sharp or pointed objects to press buttons on the touchscreen
Main Screen
The Main screen appears after the instrument has been switched on.
1
Disinfection (Cold D Function) 9 Favourite Temperatures
2
Cryobar 10 Vacutome Settings
3
Menu 11 Height Adjustment
4
Trimming Thickness 12 Handwheel Brake On/Off
5
Blade Temperature 13 Multi-Function Button
6
Specimen Head Temperature 14 Cutting Mode
7
Fine Section Thickness 15 Time or Date / Service Message (Wrench symbol)
8
Cutting Window
Thermo Scientific CryoStar NX70 Operator Guide
39
• Press the required key to activate / deactivate the function or to display the options screen for the
selected function - See Setting Preferences.
"OK" Button
• Press the “OK” button to save any changes to specific settings.
"Back" Button
• Press the “Back” button to return to the previous menu without saving any changes.
"Arrow" keys
The “Arrow” keys are used in several menus to increase or decrease values.
• Use the arrow keys to adjust the value to the required setting.
1 Decreases the selected value by multiple steps 3 Increases the selected value by 1 step
2 Decreases the selected value by 1 step 4 Increases the selected value by multiple steps
Note
The number of steps by which the 'multiple step' increase / decrease buttons alter the selected value by will
vary depending on the function
Thermo Scientific CryoStar NX70 Operator Guide
40
Setting Preferences
Quick Freezing of the Specimen
The Cryobar provides on-demand, quick freezing of specimens.
Note
When the Cryobar is activated, it will take approximately 2 - 3 minutes for the Cryobar to reach a
temperature of -60°C.
The Cryobar remains active for approximately 10 minutes.
The specimen is frozen onto the specimen chuck using a freezing compound and the Cryobar.
To ensure optimal adhesion of the specimen to the chuck, store the chucks at room
temperature prior to use.
• Press the "
Cryobar
" button on the Main screen to activate it.
• The "
Cryobar
" button is highlighted in blue when the function is activated.
• To turn off the Cryobar function, press the highlighted "
Cryobar
" button again.
Thermo Scientific CryoStar NX70 Operator Guide
41
Trimming and Fine Section Thickness
The trimming and fine section thickness can be set in two ways:
Using the joystick
• Tilt the joystick either to the left or to the right to toggle between "
Fine
" and "
Trimming
"
thickness settings.
Fine Section Thickness
Trim Section Thickness
Note
The selected function is displayed highlighted on the Main screen.
• Turn the joystick knob left to decrease the selected thickness.
• Turn the joystick knob right to increase the selected thickness.
• For details of the thickness steps, refer to the Technical Specifications section
.
Using the touch screen display
To set the trimming section thickness:
• Press the "
Trimming Thickness
" button on the Main screen.
• The Trim Section screen will be displayed.
Thermo Scientific CryoStar NX70 Operator Guide
42
• Either select one of the pre-set values (400µm, 200µm, 100µm or 10µm) or adjust the value
using the arrow keys.
• For details of the thickness steps, refer to the Technical Specifications section
.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
To set the fine section thickness:
Press the "
Fine Section Thickness
" button on the Main screen.
The Fine Section screen will be displayed.
• Either select one of the pre-set values (100µm, 50µm, 10µm or 5µm) or adjust the value using
the arrow keys.
• For details of the thickness steps, refer to the Technical Specifications section
.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
43
Temperature
Both the specimen head and the blade holder are actively cooled and the user can adjust either
temperature independently.
Setting the temperature of the specimen head
• Press the "
Specimen Head Temperature
" button on the Main screen.
• The Temperature Specimen Head screen will be displayed.
• Either select one of the pre-set temperatures (-40°C, -30°C, -20°C or -10°C) or adjust the
temperature using the arrow keys.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
Note
The new set temperature is displayed in white on the Main screen until the temperature is reached.
Thermo Scientific CryoStar NX70 Operator Guide
44
Set the Blade Temperature
• Press the "
Blade Temperature
" button on the Main screen.
• The Temperature Blade screen will be displayed.
• Either select one of the pre-set temperatures (-35°C, -30°C, -20°C or -10°C) or adjust the
temperature using the arrow keys.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
Note
The new set temperature is displayed in white on the Main screen until the temperature is reached.
Thermo Scientific CryoStar NX70 Operator Guide
45
Cutting Window
The cutting window allows the user to customise this setting for each specimen.
• On the Main screen press the "
Cutting Window
" button.
• The Set Cutting Window screen will be displayed.
To set the lower limit of the cutting window
• Turn the handwheel so that the lower edge of the specimen is positioned slightly above the blade
edge.
• Press the “Start" button.
• The lower limit should now be set and the "Start" button is highlighted in yellow.
To set the upper limit of the cutting window
• Continue turning the handwheel clockwise so that the upper edge of the specimen is positioned
just below the blade edge.
• Press the “End" button.
• The "End" button is highlighted in yellow.
Next Steps
• Press "OK" to confirm the setting and to
return to the Main screen.
• Press the "Back" button to exit back to the
Main screen without saving the changes.
• Press the “Start/Stop” button on the right
control panel twice to start sectioning.
Note
A cutting window should only be set during the downward stroke.
Thermo Scientific CryoStar NX70 Operator Guide
46
Select a Favourite
Favourites are user-definable, pre-selectable temperature settings for the specimen head and the blade
holder.
To select one of the pre-set Favourites:
• Press the "
Favourites
" button on the Main screen.
• The Favourites sliding menu will appear.
• Press the desired favourite to select it.
• The sliding window disappears and the selected Favourite is highlighted in yellow.
• To program favourites, refer to the Setting Preferences – Favourites section
.
Note
The sliding menu disappears after approximately 3 seconds if no Favourite is selected.
Thermo Scientific CryoStar NX70 Operator Guide
47
Vacutome and Cleaning Mode
The Vacutome system provides an active vacuum that aids in chamber cleaning and stretching of sections.
Note
The user sets the desired value depending upon the intended function (stretching or suction process), section
thickness and the size of the specimen.
The vacuum applied for stretching sections and/or disposing of section waste is only active when within the
cutting window. Outside of the cutting window, the selected vacuum is turned off by means of a valve.
After 10 minutes of inactivity the vacuum will automatically switch off. When activity resumes the vacuum
will automatically switch on. The vacuum takes approximately 2 seconds to reach full efficiency.
The Vacutome system is provided with two different-length nozzles. The longer nozzle is optimized for use in
specimen trimming and aids in keeping the Cryochamber clean. The shorter nozzle is optimized for use with
the anti-roll plate in appropriately stretching specimens.
• Press the "
Vacutome
" button on the Main screen.
• The Vacutome screen will be displayed.
Thermo Scientific CryoStar NX70 Operator Guide
48
Activate the Vacutome
To activate the Vacutome:
• Press "
Vacutome
" to display the Vacutome settings screen.
• Use the arrow keys to increase or decrease the Vacutome suction value - the default setting is
100%.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
Note
The Vacutome suction setting can also be adjusted by turning the Joystick as long as the Vacutome screen is
displayed.
• Turn the Joystick to the left to decrease the suction value in 1% increments.
• Turn the Joystick to the right to increase the suction value in 1% increments.
Note
When the Vacutome is activated the "Vacutome" button on the Main screen will appear yellow.
Thermo Scientific CryoStar NX70 Operator Guide
49
Turning off the Vacutome function
• Press the yellow "
Vacutome
" button on the Main screen.
• Press the yellow "
Vacutome
" button on the Vacutome screen - the button will now appear white.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
Turning On the cleaning mode
To activate the cleaning mode:
• Press the "
Vacutome
" button on the Main screen.
• The Vacutome screen will be displayed.
• Press "
Cleaning Mod e
" to activate this mode.
Note
The "Cleaning Mode" button turns yellow to indicate that this function is now activated.
The cleaning mode will switch of automatically after 10 minutes.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
• To clean the cooling chamber, remove the Vacutome hose from the nozzle plug and then:
I. Wash and dry the cooling chamber using a cloth or paper towel, and appropriate cleaning
agents.
Thermo Scientific CryoStar NX70 Operator Guide
50
II. Attach the hose extension (standard accessory 435560) to the Vacutome hose and vacuum
clean the small parts. The hose extension provides enough length to reach all parts of the
cooling chamber.
Turning Off the cleaning mode
To deactivate the cleaning mode:
• Press the yellow "
Vacutome
" button on the Main screen.
• Press the yellow "
Cleaning Mode
" button on the Vacutome settings screen - the button will now
appear white.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
• Re-attach the Vacutome hose to the blade holder.
Thermo Scientific CryoStar NX70 Operator Guide
51
Height Adjustment
The CryoStar NX70 cryostat has an electronic height adjustment which enables the user to raise or lower
the cryostat to a convenient working height.
Ensure that the area to the rear of the CryoStar is free of obstructions to avoid damaging
the instrument when the height adjustment is in use.
Do not lean on the instrument when the height adjustment is carried out to avoid damage
to the instrument.
Ensure that the power cord is free to move.
To adjust the height of the cryostat:
• Press the "
Height
" button on the touch screen.
• An adjustment menu will appear.
• Use the arrow buttons to raise or lower the cryostat to the required level.
• Press "
OK
" to confirm the adjustment. The sliding menu disappears after approximately 3
seconds if "
OK
" is not pressed.
Note
Continuous use of the height adjustment for longer than 1 minute will result in a pause of 9 minutes before
the function can be used again.
Thermo Scientific CryoStar NX70 Operator Guide
52
Multi-Function Bar
The multi-function bar provides the user access to useful information and functions.
The Multi-Function Bar is located at bottom of the Main screen.
1 Time / Date / Service Message 3 Multifunction Button
2 Cutting Mode 4 Brake
Time and Date
To toggle between Time or Date indication:
Press the "Time / Date" button.
Note
When there is an outstanding service message, a wrench symbol is show instead of the Date / Time button.
• Press the Wrench symbol to see the service message.
Thermo Scientific CryoStar NX70 Operator Guide
53
Cutting Mode Selection
The cutting mode selection aids in utilizing the motorized cutting function. Four cutting modes for the
motorized cutting drive are available.
• Single
• Interval
• Multi-Stroke
• Continuous-Stroke
Note
In case of emergency, the motor drive can be stopped in every operating mode by pressing the emergency stop
button or the emergency stop in the (optional) foot pedal. To continue sectioning pull out the emergency stop
button or unlock the emergency stop in the foot pedal.
The cutting mode can be changed in three ways. Via the Main screen, in the cutting mode submenu or
via the mode button on the right touch panel.
To switch between the cutting modes using the Main screen:
• Press the "
Multi-Function
" button on the Main screen to switch between the cutting modes.
Thermo Scientific CryoStar NX70 Operator Guide
54
To switch between the cutting modes using the cutting mode submenu:
• Press the "
Menu
" button on the Main screen.
• The Menu screen will be displayed.
• Press the "
Cuttin g Mode
" button.
• The Menu: Cutting Mode screen will be displayed.
• Press on the desired cutting mode (Continuous, Interval, Single or Multi).
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button to exit back to the Main screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
55
To switch between the cutting modes using the "Mode Selection" button on the right touch panel:
• Press the mode selection button on the left touch panel.
• The actual cutting mode is displayed on the multi function bar on the Main screen.
Mode: Single
By using this mode, a single full cutting movement is carried out with the specimen head stopping in the
down position.
• Press either the "Start/Stop" button twice or press the foot pedal twice to carry out a single
cutting stroke.
Mode: Interval
The cutting movement is carried out as long as the Start/Stop button or the foot pedal is pressed. The
movement can be stopped in any position.
• Press either the "Start/Stop" button twice or press the foot pedal twice.
• Hold the "Start/Stop" button or the foot pedal on the second press.
• Release either the "Start/Stop" button or the foot pedal to stop cutting immediately.
Thermo Scientific CryoStar NX70 Operator Guide
56
Mode: Multi-Stroke
A pre-set number of strokes will be carried out.
Note
When "Multi-Stroke" is first selected, the default number of strokes is two.
To set the number of strokes for the Multi-Stroke Mode:
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Cutting Mode" button.
• The Menu: Cutting Mode screen will be displayed.
• Press the "Multi" button to highlight it.
Use the arrow keys to select the number of strokes.
Thermo Scientific CryoStar NX70 Operator Guide
57
Note
The default number of cutting strokes is 2.
The minimum number of strokes is 2, the maximum number is 99.
• Press "OK" to confirm the setting and to return to the Main screen.
• Press the "Back" button twice to exit back to the Main screen without saving the changes.
To carry out Multi-Stroke sectioning:
• Set the number of strokes as described above.
• Press either the "Start/Stop" button twice or press the foot pedal twice to start sectioning.
• The cutting movement stops automatically after the pre-set number of strokes have be en carried
out.
Mode: Continuous-Stroke
An unlimited number of strokes will be performed when this mode is selected.
To start the continuous-stroke mode:
• Press either the "Start/Stop" button twice or press the foot pedal twice.
• To stop the continuous-stroke, press the "Start/Stop" button or the foot pedal again.
• The cutting drive will stop after the next full downward stroke
Thermo Scientific CryoStar NX70 Operator Guide
58
Handwheel Brake
Always activate the brake when manipulating the blade/knife and specimen. For your personal
safety, also activate the handwheel mechanical brake.
To activate the Handwheel Brake:
Press the "Brake" button on the Main screen or press the "Brake" button on the right touch pad.
Note
The "Brake" button is highlighted if the handwheel brake is activated.
To deactivate the Handwheel Brake:
Press the red highlighted "Brake" button on the Main screen or the "Brake" button on the right touch
pad.
Thermo Scientific CryoStar NX70 Operator Guide
59
Multi-Function Button
The Multi-Function button provides to the user additional information on the sectioning process.
• Four different indications can be displayed by pressing this button.
Remaining Distance to the Front End Position
This button indicates the remaining distance to front end position of the blade holder.
Section Thickness Sum
This button indicates the total section thickness sum of the actual mounted specimen.
• To reset this value, press this button and keep it pressed for approximately 3 seconds.
Note
It is recommended to reset the section thickness sum if a new specimen is mounted in the specimen head for
sectioning.
Motorized Cutting Drive
The motorized cutting drive facilitates routine work and ensures a steady cutting speed even for hard
specimens.
When using the motorized cutting drive the hand wheel is controlled automatically.
Thermo Scientific CryoStar NX70 Operator Guide
60
Setting the Cutting Speed
To set the speed at which motorized cutting drive will operate:
• Select the Speed settings on the multi-function bar on the Main screen.
• The speed can be varied from 0 to 100 using the knob on the right hand site of the instrument.
• Turning the knob to the left decreases the speed; turning to the right increases the speed.
Note
The selected speed will be used in the cutting window. Outside of the cutting window a higher return speed
determined by the system is used.
• Use either the operating controls or the foot pedal (if fitted) to turn on/off the drive.
• The cutting speed can be varied to allow it to better suit to the properties of the specimen.
Setting a cutting speed too high in combination with a high section thickness, may result in a
warning screen.
Section Counter
The section counter adds up the number of the produced sections. After each downward movement of the
specimen head, the number on the section counter increases by 1.
To reset the section counter:
• Press the Section Counter button for approximately 3 seconds to reset it to zero.
Thermo Scientific CryoStar NX70 Operator Guide
61
Menu
The "Menu" button provides access to submenus for adjustments, settings and the user guide.
To enter the Menu screen:
• Press the "
Menu
" button.
• The Menu screen will be displayed.
• Press the "
Back
" button to return to the Main screen.
• Press the desired function button to access the respective submenu.
Thermo Scientific CryoStar NX70 Operator Guide
62
Chamber Light
To adjust the brightness of the chamber light:
• Press the "
Menu
" button on the Main screen.
• The Menu screen will appear.
• Press the "
Chamber Light
" button.
• The Menu: Chamber Light screen will appear.
• Select one of the pre-set values (25%, 50%, 75% or 100%) or adjust the brightness using the
arrow keys.
• Press "
OK
" to confirm the setting and to return to the Main screen.
• Press the "
Back
" button twice to exit back to the Main screen without saving the changes.
Note
The chamber illumination can be adjusted; from the lowest (1%) to the highest (100%) level.
Thermo Scientific CryoStar NX70 Operator Guide
63
Standby
When the CryoStar is switched on but has been inactive for 1 hour, it will automatically enter the
Standby mode. After 3 more hours, the instrument enters the sleep mode. The cryostat maintains the user
adjusted temperatures for both blade holder and specimen head during Standby. When the cryostat enters
the sleep mode, the temperature for the blade holder is set to -10°C and for the specimen head to -10°C.
In Standby mode the chamber light switches off and the touchscreen display will dim.
To enter the Standby-mode:
• Press the "
Menu
" button on the Main screen.
• The Menu screen will appear.
• Press the "
Standby
" button to switch the instrument immediately into the Standby mode.
• Press the touchscreen to exit the Standby or Sleep mode.
Thermo Scientific CryoStar NX70 Operator Guide
64
Display Lock
Note
The display lock is used to avoid unwanted changes to the settings of the cryostat.
To activate the Display Lock:
• Press the "Menu" button on the Main screen.
• The Menu screen will appear.
• Press “Display Lock” to activate the display lock immediately.
To deactivate the Display Lock:
• Press the "Lock" Symbol three times.
Note
The symbol changes its colour (red - yellow - green) each time it is pressed.
Thermo Scientific CryoStar NX70 Operator Guide
65
Favourites
Favourites are a set of pre-set values for the specimen head and blade temperature.
The user can program three favourites to allow quick selection of the set of temperatures most often used.
Note
Whenever a temperature setting is altered the cryostat will require a period of time to reach the set
temperature. Observe the readings on the display and begin sectioning when the pre-set value is reached.
To program Favourites:
• Press the "Menu" button on the Main screen.
• The Menu screen will appear.
• Press “Favourites” to enter the menu.
• The Menu: Favourites screen will appear.
1 Pre-set 3 Title Box 4 Specimen Head Temperature
2 Pre-set 2 Title Box 5 Blade Temperature
3 Pre-set 1 Title Box 6 Alternative Indication
Thermo Scientific CryoStar NX70 Operator Guide
66
Each favourite setting is divided into four sections (from left to right):
• Favourite name (displayed on the main screen if highlighted).
• Specimen head temperature.
• Blade temperature.
• Alternative indication (displayed on the main screen if highlighted).
To change the Favourite name:
• Press the name of the pre-set to be altered.
• The Keyboard screen will appear.
• Use the upper left button to toggle between capital letters, small letters and numbers.
• Erase letters or numbers by pressing the "Delete" button.
• Type in the new name for the favourite (for example “Muscle” / “Fat” / “Bone”).
• Press "OK" to confirm the setting and to return to the Favourites menu.
• Press the "Back" button to exit back to the Favourites menu without saving the changes.
Note
If the value for either the specimen head temperature or blade temperature is changed on the Main Screen,
the favourite is no longer displayed but settings are still stored in memory.
Thermo Scientific CryoStar NX70 Operator Guide
67
User Guide
This function contains an electronic version of this user guide.
To activate the User Guide:
• Press the "Menu" button on the Main screen.
• The menu screen will appear.
• Press the “User Guide” button.
• The Menu: User Guide screen will appear.
• Use the scroll bar on the right side or the two arrow keys to scroll through the manual.
• Press the "Home" button to return to the first page of the User Guide.
• Use the arrow keys to browse forward or backwards through the User Guide.
Thermo Scientific CryoStar NX70 Operator Guide
68
• Press the "Back" button to return to the settings menu screen.
• Press the "Back" button twice to return to the Main screen.
Thermo Scientific CryoStar NX70 Operator Guide
69
Settings Menu
To enter the Settings menu:
• Press the "Menu" button on the Main screen.
• The menu screen will appear.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Back" button to return to the Menu screen.
• Press the "Back" button twice to return to the Main screen.
Thermo Scientific CryoStar NX70 Operator Guide
70
Language
To set the Language:
• Press the "Menu" button on the Main screen.
• The menu screen will appear.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Language" button.
• The Menu: Settings: Language screen will appear.
• Select the desired language by pressing the respective button.
• Press "OK" to save the changes and to return to the Main screen.
• Press the "Back" button three times to return to the Main screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
71
Time and Date
Note
If a Defrost or Cold D cycle is running, the cycle must finish before the time or date can be adjusted.
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Settings Menu screen will be displayed.
Set the Date
• Press the "Date" button.
• The Menu: Settings: Date screen will appear.
Set the Day
• Press the "Day" button to highlight it.
• Use the arrow keys to select the desired day and date.
Set the Month
• Press the "Month" button to highlight it.
• Use the arrow keys to select the desired month.
Thermo Scientific CryoStar NX70 Operator Guide
72
Set the Year
Press the "Year" button to highlight it.
Use the arrow keys to select the desired year.
Press "OK" to confirm the setting and to return to the Main screen.
Press the "Back" button three times to exit back to the Main screen without saving the changes.
Change to ISO format
Press the "ISO" button to toggle between the full date format and the ISO format.
• Press "OK" to confirm the setting and to return to the Main screen.
• Press the "Back" button three times to exit back to the Main screen without saving the changes.
Setting the Time
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Time" button.
Thermo Scientific CryoStar NX70 Operator Guide
73
• The Menu: Settings: Time screen will be displayed.
Set the minutes
• Press the "Minute" button to highlight it.
• Use the arrow keys to select the desired minute.
Set the hour
• Press the "Hour" button to highlight it.
• Use the arrow keys to select the desired hour.
Set the time format
• Press the "12/24" button to toggle between the 12 and 24 hour modes.
Note
The 12/24 button is highlighted in yellow if the 12h mode is active.
• Press "OK" to confirm the setting and to return to the Main screen.
• Press the "Back" button three times to exit back to the Main screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
74
Start Time
The Start Time is the time were the cryostat switches from the sleep mode to the Active mode on every
programmed day.
To set the Start Time:
• Press the "Menu" button on the Main screen.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Start Time" button.
• The Menu: Settings: Start Time screen will appear.
• In this menu, the user can specify the day(s) and the exact time when the cryostat should start
automatically.
Note
The pre-set start time is from Monday to Sunday at 06:00 a.m.
To set the Day:
• Press the “Day” button.
Thermo Scientific CryoStar NX70 Operator Guide
75
• The Menu: Settings: Start Time screen will appear.
Note
The default setting is every day, from Monday to Sunday.
• Press the weekdays on which the cryostat should not start automatically.
Note
Deactivated weekdays will turn white.
• Press the "Hour" and "Minute" button and use the arrow keys to adjust the time at which the
cryostat should start.
• When all settings have been done, press “OK” to save the settings and to return to the Main
screen or press the "Back" button three times to return to the Main screen.
Thermo Scientific CryoStar NX70 Operator Guide
76
Defrost
Inevitably, when working on the microtome the dry cold air of the Cryochamber mixes with the warm
humid air outside the chamber. Frost forms on the finned evaporator. The increasing thickness of the
frost reduces the efficiency of the evaporator. For this reason, the daily defrosting is necessary.
During defrosting, remove the specimens from the Cryochamber, as the temperature
inside the chamber will increase. Do not leave or store specimens inside the cryostat
over a long period of time. Due to a power failure or other unexpected malfunctions of
the instrument, the specimen might be damage.
Note
Set the time of the daily defrosting on the control panel. The period of the defrosting takes approximately 1
hour and varies according to the thickness of the frost.
Possible frost build-up on the microtome or on the knife carrier is not removed by defrosting the instrument.
However, the frost recedes after defrosting as now the evaporator is fully efficient again.
In addition, the instrument is equipped with an immediate defrosting function. The immediate defrosting
takes approximately 90 minutes.
Immediate Defrosting
To start the defrosting immediately:
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Defrost" button.
• The Menu: Settings: Defrost screen will be displayed.
Thermo Scientific CryoStar NX70 Operator Guide
77
• Press the "Back" button three times to return to the Main screen without starting the defrosting.
• Press the "Now" button to start the defrosting immediately.
• The Defrosting screen will appear, showing the progress of the defrosting and the actual
temperature of the evaporator.
To abort the defrosting
• Press the "Abort" button on the Defrosting progress screen.
• The Main screen will appear, displaying an error message.
Press the "OK" button to remove the error message.
Note
The Defrosting cycle can be aborted at any time.
Thermo Scientific CryoStar NX70 Operator Guide
78
Programming the Daily Defrost Cycle
Note
The time for the daily defrost cycle should be programmed outside of the normal working hours to avoid
delays in the workflow.
To program the Daily Defrost Cycle:
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Defrost" button.
• The Menu: Settings: Defrost screen will be displayed.
• Press the "Time" button twice.
• The Menu: Settings: Defrost-Time screen will appear.
Thermo Scientific CryoStar NX70 Operator Guide
79
Note
The standard setting for the daily defrost cycle is daily at 00:00 a.m.
• To change the standard setting, press the "Hour" and / or the "Minute" button.
• Adjust the time by using the arrow keys.
• Press the "OK" button to confirm your settings.
• Press the "Back" button three times to return to the Main screen.
Thermo Scientific CryoStar NX70 Operator Guide
80
Screen
The brightness of the Screen can be adjusted.
To set the Screen brightness:
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Screen" button.
• The Menu: Settings: Screen will be displayed.
• Select one of the pre-set values (25%, 50%, 75% or 100%) or adjust the brightness using the
arrow keys.
• Press "OK" to confirm the setting.
• Press the "Back" button three times to return to the Main screen without saving the changes.
Note
The chamber illumination can be adjusted; from the lowest (1%) to the highest (100%) levels.
Thermo Scientific CryoStar NX70 Operator Guide
81
Retraction
If required, the Retraction function can be turned off.
To toggle the retraction on or off:
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Retraction" button.
• The Menu: Settings: Retraction screen will be displayed.
• Press the "OK" button to turn off the Retraction and to return to the Main screen.
• Press the "Back" button three times to return to the Main screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
82
To turn on the Retraction:
• Enter the Retraction menu screen as described above.
• Press the OK" button on the Menu: Settings: Retraction screen to turn on the Retraction and to
return to the Main screen.
• Press the "Back" button three times to return to the Main screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
83
Data Log
The Data Log records all events and error messages on the cryostat. The Data Log file can be exported to
a USB-Stick for Service purposes in case of a malfunction.
To enter the Data Log
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Data Log" button.
• The Menu: Settings: Data Log screen will be displayed.
• Use the scroll bar on the right side to scroll through the Data Log.
• Insert the USB-Stick.
Note
The "Export" button is now highlighted in white.
• Press the "Export" button to transfer the log data to the USB-Stick.
• Press the "Back" button three times to return to the Main screen.
Note
The Data Log file can only be exported to the USB-Stick supplied with the instrument.
Thermo Scientific CryoStar NX70 Operator Guide
84
Default Settings
If required, the instrument can be reset to the default settings.
To restore the Default Settings:
• Press the "Menu" button on the Main screen.
• The Menu screen will be displayed.
• Press the "Settings" button.
• The Menu: Settings screen will appear.
• Press the "Default Settings" button.
• The Menu: Settings: Default Settings screen will be displayed.
• Press the "OK" button to restore the default settings and to return the Main screen.
• Press the "Back" button three times to return to the Main screen without restoring the default
settings.
Note
A message appears on the Main screen to inform the user that the default settings have been restored. The
message disappears after approximately 3 seconds.
Thermo Scientific CryoStar NX70 Operator Guide
85
Display icons with text
The text underneath the Main screen icons can be switched off if required.
To switch the text off:
• Enter the Menu: Settings: Default Settings screen as described above.
• Uncheck the box and press the "Back" button three times to save the changes and to return to the
Main screen.
Pressing "OK" will reset the instrument to the default settings.
Service
This submenu is only for service purposes. A password is required to enter the Service menu.
Thermo Scientific CryoStar NX70 Operator Guide
86
Emergency Stop
Standard Emergency Stop Function
To activate the emergency stop:
Press the red emergency stop button to immediately stop the motorized cutting drive.
Note
When the emergency stop is activated, the handwheel brake button in the display and the handwheel brake
button on the right keypad light up and a red button are shown on the display.
• To reset the emergency stop function pull out the red emergency stop button.
• The motorized cutting drive can then be started again.
Thermo Scientific CryoStar NX70 Operator Guide
87
Optional Foot Pedal Emergency Stop
A second emergency stop device is integrated into the optional foot pedal.
To activate the emergency stop using this method:
• Firmly step on the foot pedal to immediately stop the motorized cutting drive.
Notes
The button for the handwheel brake does not light up when the foot pedal emergency stop is activated,
however the display will show a red button as per the standard emergency stop method.
This emergency stop device will remain active as long as the foot pedal is engaged.
• The motorized cutting drive can then be started again.
Cooling Processes
Specimen and blade holder cooling
Integrated refrigeration systems allow the blade holder to be cooled to -35°C and the specimen head to be
cooled to -50°C. Specimen stations are located on the left side inside the Cryochamber.
These specimen stations passively cool specimens to a temperature of -30°C and can be used as a staging
area.
The temperature range of the specimen head is adjustable between +10°C and -50°C.
The instrument is also equipped with an active Cryobar.
The Cryobar area actively cools down to -60°C.
Note
Use the heat extractor to accelerate the freezing of the specimen.
Thermo Scientific CryoStar NX70 Operator Guide
88
Sectioning Procedures
Cutting Movement and Retraction
Front Rear
Lateral view into instrument
Note
The retraction prevents the surface of the specimen being damaged during the return stroke of the specimen
head.
The retraction can be switched off - see "Switching the Retraction On - Off".
The retraction distance required is 20 microns and cannot be changed.
Thermo Scientific CryoStar NX70 Operator Guide
89
Trimming and First Cuts
After the specimen orientation and the blade angle have been set, trimming can be carried out using the
trimming function.
Trimming
• Make sure that the trimming thickness setting is selected by using the joystick.
Note
Trimming thickness is shown highlighted on the display.
• Turn the hand-wheel in a clockwise direction to begin trimming.
• Press the "Trim" button to move forward the blade holder by the pre-set trim value. The hand-
wheel does not need to be turned for this function.
Note
The blade holder will move forwards by the pre-set trim thickness value every cycle.
Thermo Scientific CryoStar NX70 Operator Guide
90
Sectioning and Taking Off Sections
• Set the desired section thickness - see Cutting Thickness
• Set the desired vacuum level - see Vacutome and Cleaning Mode.
• Place the anti-roll plate against the
blade.
• Turn the hand wheel in a
clockwise direction or use the
motorized cutting drive to carry
out sectioning.
Note
The section slides into the space between the blade and the anti-roll plate.
• Use the handle to move the anti-roll plate assembly away from the specimen.
• The section should remain on the clamping plate.
• Arrange specimen section gently with a brush.
• Transfer the specimen onto a glass microscope slide with help of adhesion forces.
Thermo Scientific CryoStar NX70 Operator Guide
91
Handwheel Brakes
Before placing or removing a specimen on the specimen head, or adjusting the orientation, always ensure
that the mechanical handwheel brake is engaged. The cryostat is equipped with an electronic and a
mechanical handwheel brake to prevent unintended movement of the specimen head.
Activating the brakes reduces the risk of being injured when adjusting the specimen head and/or the blade
carrier.
When the instrument is turned off, the electronic hand wheel brake cannot be activated!
Whenever the instrument is turned off, the mechanical hand wheel brake has to be
activated.
For your personal safety, both electronic and mechanical hand wheel brakes must always
be activated when working on the specimen head or blade carrier.
Electronic Handwheel Brake
To activate the electronic Handwheel Brake
• Press the "Brake" button on the Main screen.
• The "Brake" button is highlighted in red to indicate that the electronic br ake is active.
Note
The motorized cutting drive cannot be started if the electronic Handwheel brake is activated.
To release the electronic Handwheel Brake
• Press the "Brake" button on the Main screen again.
• The "Brake" button is highlighted in white to indicate that the electronic Handwheel Brake is
now deactivated.
Note
For safety reasons, the brake function is automatically activated after each stop of the motorized cutting
drive.
Thermo Scientific CryoStar NX70 Operator Guide
92
Mechanical Handwheel Brake
The mechanical handwheel brake acts as an additional safety device, especially when the instrument is
turned off and the electronic handwheel brake is inoperable. The mechanical handwheel brake can be
activated by a switch on the handwheel.
To activate the mechanical Handwheel Brake
• Bring the handwheel handle into a 12
o'clock position.
• The switch for the handwheel brake can
now be pressed into the locked position.
Note
If the switch is not in the correct position to lock, keep the switch pressed and move the handwheel slowly
forward and backwards until the switch is locked.
Starting the cutting motor drive is not possible when the instrument is turned off or when the mechanical
hand wheel brake is activated.
• To unlock the mechanical Handwheel
Brake:
• Pull out the Handwheel Brake switch.
Thermo Scientific CryoStar NX70 Operator Guide
93
Cold D
Starting the Disinfection (Cold D) Function
The Cold D function allows the user to disinfect the Cryochamber with Sanosil®. Disinfection is either
on-demand or pre-programmed.
The Cold D cycle takes 50 minutes to complete.
The window of the Cryochamber must always be closed before starting and during a Cold
D cycle.
Note
If the window is not closed, the warning “Window not closed” will be displayed.
Before starting the Cold D function, remove all specimens from the Cryochamber
• Press the “Disinfect” button on the Main screen.
• The Disinfection screen will appear.
• Press "OK" to start the disinfection immediately.
• Press the "Back" button to return to the Main screen without starting the disinfection.
Note
Pressing the "OK" button starts immediate disinfection of the cryostat chamber, regardless of the
programmed times.
• The Disinfection Remaining Time screen will appear.
Thermo Scientific CryoStar NX70 Operator Guide
94
Note
After the disinfection cycle is completed, the Disinfection Remaining Time screen will disappear and the
Main screen will be displayed.
A small amount of liquid and, occasionally, a small amount of icing remain in the Cryochamber following
completion of a Cold D cycle. These by-products of the Cold D cycle will normally evaporate from the
Cryochamber within a period of three hours. The liquid droplets can be removed with a paper towel and, if
necessary, any icing can be removed with an alcohol spray. Neither the liquid droplets nor the icing will
prevent the use of the cryostat immediately after completion of a Cold D cycle.
Any occasional build-up of crystalline material in the Cryochamber can also be easily removed with an
alcohol spray and paper towel.
Thermo Scientific CryoStar NX70 Operator Guide
95
Aborting the Disinfection
Note
The disinfection process can be aborted at any time to facilitate an urgent frozen section.
To abort the disinfection process:
• Press the "Abort" button on the Disinfection Remaining Time screen.
Note
If the Disinfection cycle was aborted, an error message appears on the Main screen.
• Press the "OK" button to remove the error message.
An interrupted Cold D cycle will not adequately disinfect the Cryochamber. However,
the instrument can be used immediately afterwards.
Thermo Scientific CryoStar NX70 Operator Guide
96
Programming Cold D
In addition to the instant use, automatic Cold D cycles can be programmed.
Follow the directions below to activate (or deactivate) the automatic Cold D feature.
• Press the "Menu" button on the Main screen.
• Press the "Settings" button.
• The Settings Menu screen will be displayed.
• Press the "Disinfection" button.
• The Menu: Settings: Disinfection scree n will appear.
• In this menu, the user can specify the day(s) and the specific time when the Cold D process
should automatically be carried out.
To set the Day:
• Press the “Day” button.
• The Menu: Settings: Disinfection day selection screen will appear.
Thermo Scientific CryoStar NX70 Operator Guide
97
Note
The default setting is every day, from Monday to Sunday at 3:00 am.
• Press the weekdays on which the automatic disinfection should not be carried out.
Note
Deactivated weekdays will turn white.
• Press the "Hour" and "Minute" button and use the arrow keys to adjust the time at which the
Cold D cyc le should start.
• When all settings have been done, press "OK" to save the settings and to return to the Main
screen.
• Press the "Back" button to return to the Setting screen without saving the changes.
Thermo Scientific CryoStar NX70 Operator Guide
98
Chapter 5 - Troubleshooting
Troubleshooting Tables
Problem
Possible Reason
Advice
Sections fold or
crumple
1. Specimen is too warm. Lower specimen tempe rature
2. Knife holder too warm. Lower chamber temperature.
Tip – use freezer spray on clamp plate and blade to
confirm.
3. Anti-Roll plate too low. Raise Anti-Roll towards specimen.
4. Anti-Roll plate and/or clamp
plate dirty.
Clean with absolute alcohol & dry thoroughly.
Section rolls up
under the Anti-
Roll
plate
1. Specimen is too cold. Raise specimen temperature.
Specimen curls
after lifting ARP
1. Anti-Roll plate & clamp plate
too warm.
Lower chamber temperature
Tip – use freezer spray on clamp plate and blade to
confirm.
2. Static electricity in chamber.
3. Blade blunt. Change blade.
Sections tear or
crack
1. Specimen too cold. Raise temperature.
2. Blade damaged or dirty. Change blade.
3. Specimen frozen too rapidly or
specimen overly large.
Specimen and
section chatter
1. Knife holder not correctly
clamped.
Check & tighten all stages of knife holder.
2. Blade incorrectly clamped.
Blade clamping force can be increased by tightening
clamp screw at the rear of the top stage.
3. Specimen incorrectly clamped.
Check specimen is securely mounted and clamped in
specimen head jaws. Check there is no debris or ice on
the back of the chuck or on specimen head.
Thermo Scientific CryoStar NX70 Operator Guide
99
Problem
Possible Reason
Advice
1. Check knife holder and
specimen correctly clamped.
2. Ensure specimen is securely
attached to the
Cryocassette/chuck.
3. Temperature of specimen
incorrect.
Raise or lower temperature.
4. Blade not sharp. Change blade.
Thermo Scientific CryoStar NX70 Operator Guide
100
Tips for Successful Sectioning
For optimal sectioning, the following points should be followed:
• Check the condition of the blade edge; move it horizontally to the left or right side to obtain a
sharp cutting edge.
• Check adjustment of anti-roll plate and correct it, if necessary.
• Ensure that the edge of the anti-roll plate glass is clean and free of debris.
• Carefully remove frost from the front and the rear part of the blade, from the anti-roll plate and
the clamping plate (i.e. with ethanol).
• Tighten all clamping screws and clamping levers on the blade holder and specimen head.
• Select the appropriate knife carrier and specimen temperature according to the specimen type -
see Optimum Temperature Ranges for Sectioning Different Types of Fresh Specimens.
• Allow time for the temperatures within the chamber to stabilise.
• Select the appropriate freezing compound (Neg50).
• Avoid excessive use of freezing compound.
Note
If the specimen was frozen with liquid nitrogen or similar freezing techniques, the specimen must be allowed
to adjust to the cutting temperature.
• Adjustment of proper clearance angle. Select a clearance angle of 8-12°. The preset clearance
angle is 10°.
• Select an appropriate cutting speed: The harder the material, the slower the cutting speed!
• Take care when bringing knife and specimen together.
During defrosting, remove the specimens from the cryochamber, as the temperature
inside the chamber will increase. Do not leave or store specimens inside the cryostat
over a long period of time. Due to a power failure or other unexpected malfunctions of
the instrument, the specimen might be damaged.
Loading...