Thermo Scientific CryoStar NX70 series, CryoStar NX70 HOMPD, CryoStar NX70 HOMP, CryoStar NX70 HOMPV, CryoStar NX70 HOMPDV Operator's Manual
Thermo Scientific CryoStar NX70
Product Code
956960, 956970, 956980, 956990, 957000, 957010,
957020, 957030, 957040, 957050, 957060, 957070
Operator Guide
387928 Issue 10
Thermo Scientific CryoStar NX70 Operator Guide
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Thermo Scientific CryoStar NX70 Operator Guide
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Company Information
© Copyright 2016. Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. (Thermo Fisher Scientific) is the world leader in serving science, offering a
unique combination of innovative technologies. Thermo Scientific is a brand name of Thermo Fisher
Scientific.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting
document is correct and clearly stated, but does not accept responsibility for any errors or omissions. The
development of Thermo Scientific products and services is an ongoing process. Please ensure that any
published information you use as a reference is up to date and relates to the condition of the product. If
necessary, check with your local Thermo Fisher Scientific representative.
This document may not, in whole or in part, be copied, photocopied, reproduced, translated, or
converted to any electronic or other form without prior written consent of Thermo Fisher Scientific. All
information contained in this manual is proprietary and confidential, and the exclusive property of
Thermo Fisher Scientific and is protected by copyright.
Contact Addresses:
Thermo Shandon Limited (Trading as Thermo Fisher Scientific),
Tudor Road, Manor Park,
Runcorn, WA7 1TA, UK
Tel: +44 (0) 1928 534 000; Fax: +44 (0) 1928 534 001
Email: sales.ap.uk@thermofisher.com
Web: http://www.thermoscientific.com/pathology
USA Distributor:
Richard-Allan Scientific
Subsidiary of Thermo Fisher Scientific
4481 Campus drive
Kalamazoo
MI 49008
USA
This instrument conforms to the essential requirements of:
IVD Directive 98/79/EC
and its harmonized standards under Union harmonization legislation
Thermo Scientific CryoStar NX70 Operator Guide
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NX70 110V
EC Regulation No 517/2014 – Fluorinated Greenhouse Gas Regulations
Conta ins fluorina ted greenhouse ga ses
Refrigerant: R404A
R404A GWP value = 3992
System charge weight = 0.400 Kg
System CO2 equiva lent charge weight = 1.597 tonnes
NX70 230V
EC Regulation No 517/2014 – Fluorinated Greenhouse Gas Regulations
Conta i ns fluorina ted greenhouse ga ses
Refrigerant: R404A
R404A GWP value = 3992
System charge weight = 0.400 Kg
System CO2 equiva lent charge weight = 1.597 tonnes
EMC Statement
This IVD equipment complies with the emissions and immunity requirements of IEC 61326-2-6:2012
and IEC 61326-1:2012.
This equipment has been designed and tested to CISPR 11:2009+A1:2010 Class A.
It is intended for use in a laboratory environment by a trained and qualified professional. In a domestic
environment it may cause radio interference, in which case it may be necessary to take measures to
mitigate the interference.
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Important Note Regarding Instrument Variants
This document is designed for use with all variants of the Thermo Scientific CryoStar NX70 cryostat.
A range of optional features are available on this instrument and the instructions contained in this manual
are not specific to any one variant. Thus some features described may not be available on your instrument.
This IFU is valid for the variants listed below:
Product Code Product Variant
956960 CryoStar NX70 HOMP 100V 60 Hz
956970 CryoStar NX70 HOMPD 100V 60 Hz
956980 CryoStar NX70 HOMPV 100V 60 Hz
956990 CryoStar NX70 HOMPDV 100V 60 Hz
957000 CryoStar NX70 HOMP 110-120V 60 Hz
957010 CryoStar NX70 HOMPD 110-120V 60 Hz
957020 CryoStar NX70 HOMPV 110-120V 60 Hz
957030 CryoStar NX70 HOMPDV 110-120V 60 Hz
957040 CryoStar NX70 HOMP 220-230V 50/60Hz
957050 CryoStar NX70 HOMPD 220-230V 50/60Hz
957060 CryoStar NX70 HOMPV 220-230V 50/60Hz
957070 CryoStar NX70 HOMPDV 220-230V 50/60Hz
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Symbols
The following symbols and conventions may be used throughout this document and on the instrument:
This symbol is used on the instrument, or in a document, to indicate that instructions
must be followed for safe and correct operation. If this symbol appears on the
instrument, always refer to the operator guide.
This symbol is used on the instrument, or in a document, to indicate that there are
potential biological risks associated with the instrument and / or instrument use. Always
use Good Laboratory Practice.
Cold surface, if necessary, use gloves
Cutting hazard, sharp edges, watch your fingers.
This symbol is used on the instrument, or in a document, to indicate that irritants or
potentially harmful chemicals are present. Refer to the Material Safety Data Sheets for
the products, and always use Good Laboratory Practice.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European
countries with a separate collecting system within the waste management. This product,
being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).
Manufacturer.
Serial number
It is stated on the product label sticker.
It is built up as follows: SYYMMXXXX
S=Production site, Y=year of production, M=Month of production, X= Counter
So the serial number states the production date of the device
A warning is given in the documentation if there is a danger of personal injury or damage to the
equipment or samples.
Note
Notes give additional information about a job or instruction, but do not form part of the instruction.
SN
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Table of Contents
COMPANY INF ORMATION............................................................................................................................................ 3
EMC STATEMENT .......................................................................................................................................................... 4
IMPO RTANT NOTE REGARDING INSTRUMENT VARIANTS ....................................................................................... 5
SY MB OL S ........................................................................................................................................................................ 6
TABL E OF CON TENTS..................................................................................................................................................... 7
CHAPTER 1 - SAFETY INFORMA TION ......................................................................................................................... 10
GENERAL SAFETY.......................................................................................................................................................... 10
CHEM ICAL SA FET Y ........................................................................................................................................................ 11
ENVI RONMENT............................................................................................................................................................. 11
WARRANTY STATEMENT................................................................................................................................................ 12
CHAPTER 2 - INTRO DUCTION ..................................................................................................................................... 13
INT ENDE D USE ............................................................................................................................................................. 13
INS TRUME NT FEATURES ................................................................................................................................................ 13
TECHNICAL SPECIFICATIONS ........................................................................................................................................... 14
Mechanical Specifications.................................................................................................................................. 14
Electrical Specifications ...................................................................................................................................... 14
Environmental Specifications............................................................................................................................. 14
Cooling Specifications......................................................................................................................................... 15
Microtome and Sectioning Specifications ......................................................................................................... 15
Cold D Specification ............................................................................................................................................ 16
Default Settings .................................................................................................................................................. 16
CHAPTER 3 - INSTRUMENT SETUP ............................................................................................................................. 18
UNPACKING A ND REPACKI NG ......................................................................................................................................... 18
Safety Precautions .............................................................................................................................................. 18
Unpacking Procedure ......................................................................................................................................... 18
Repacking the Instrument .................................................................................................................................. 19
LOCAT ION & SET UP ...................................................................................................................................................... 20
IDENTI FICA TION O F PA RTS ............................................................................................................................................. 21
Overview.............................................................................................................................................................. 21
Connections Panel .............................................................................................................................................. 22
Cryochamber ....................................................................................................................................................... 23
Blade Holder........................................................................................................................................................ 24
Specimen Head NX70 ......................................................................................................................................... 25
SET UP........................................................................................................................................................................ 26
Fitting or Exchanging the Anti Roll Plate .......................................................................................................... 26
Fitting or Exchanging Low Profile Blades .......................................................................................................... 27
Removing Used Blades with the Magnetic Blade Tool..................................................................................... 28
Blade Positioning and Orientation .................................................................................................................... 29
Adjusting the Cutting Angle ............................................................................................................................... 30
Adjusting the Anti-Roll P la te .............................................................................................................................. 32
SPECIMEN SETUP.......................................................................................................................................................... 33
Specimen Orientation ......................................................................................................................................... 33
FILLING WITH DISINFECTANT TO PERFO RM THE COLD D FUNCTION ..................................................................................... 35
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Filling the Cryostat with Disinfectant Solution (Sanosil®) ................................................................................ 35
CHAPTER 4 – OP ERA TION ........................................................................................................................................... 37
SWITCHING ON ............................................................................................................................................................ 37
SYSTEM INTE RFA CING ................................................................................................................................................... 38
Using the Touchscreen ....................................................................................................................................... 38
Main Screen ........................................................................................................................................................ 38
SETTING PREFERENCES .................................................................................................................................................. 40
Quick Freezing of the Specimen......................................................................................................................... 40
Trimming and Fine Section Thickness................................................................................................................ 41
Temperature ....................................................................................................................................................... 43
Set the Blade Temperature ................................................................................................................................ 44
Cutting Window .................................................................................................................................................. 45
Select a Favourite ............................................................................................................................................... 46
Vacutome and Cleaning Mode .......................................................................................................................... 47
Height Adjustment.............................................................................................................................................. 51
Multi-Fun ction Bar.............................................................................................................................................. 52
Time and Date..................................................................................................................................................... 52
Cutting Mode Selection ...................................................................................................................................... 53
Handwheel Brake................................................................................................................................................ 58
Multi-Function B utton ........................................................................................................................................ 59
Menu ................................................................................................................................................................... 61
Chamber Light..................................................................................................................................................... 62
Stand by................................................................................................................................................................ 63
Display Lock......................................................................................................................................................... 64
Favourites............................................................................................................................................................ 65
User Guide........................................................................................................................................................... 67
Settings Menu ..................................................................................................................................................... 69
Language............................................................................................................................................................. 70
Time and Date..................................................................................................................................................... 71
Start Time ............................................................................................................................................................ 74
Defrost ................................................................................................................................................................. 76
Immediate Defrosting ........................................................................................................................................ 76
Programming the Daily Defrost Cycle ............................................................................................................... 78
Screen .................................................................................................................................................................. 80
Retraction............................................................................................................................................................ 81
Data Log .............................................................................................................................................................. 83
Default Settings .................................................................................................................................................. 84
Serv ice ................................................................................................................................................................. 85
EMERGENCY STOP ........................................................................................................................................................ 86
Standard Emergency Stop Function .................................................................................................................. 86
Optional Foot Pedal Emergency Stop................................................................................................................ 87
COOLING PROCESSES .................................................................................................................................................... 87
Specimen and blade holder cooling................................................................................................................... 87
SECT IONI NG PRO CEDURES ............................................................................................................................................. 88
Cutting Movement and Retraction.................................................................................................................... 88
Trimming and First Cuts ..................................................................................................................................... 89
Trimming ............................................................................................................................................................. 89
Sectioning and Taking Off Sections ................................................................................................................... 90
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HANDWHEEL BRAKES .................................................................................................................................................... 91
Electronic Handwheel Brake .............................................................................................................................. 91
Mechanical Handwheel Brake ........................................................................................................................... 92
COLD D....................................................................................................................................................................... 93
Starting the Disinfection (Cold D) Function....................................................................................................... 93
Abo rting th e Disinfection ................................................................................................................................... 95
Programming Cold D .......................................................................................................................................... 96
CHAPTER 5 - TROUBLESHOOTING.............................................................................................................................. 98
TROUBLESHOOTING TABLES ........................................................................................................................................... 98
TIPS FOR SUCCESSFUL SECTIO NING ...............................................................................................................................100
How to avoid malfunctions when working with the Vacutome ....................................................................101
Sectioning Temperature Guidelines fo r Fresh Ti ssu es....................................................................................102
CHAPTER 6 - CLEAN ING AND MAINTENANCE.........................................................................................................103
CLEANI NG THE TO UCH SCREEN DISPLAY ........................................................................................................................103
SHUTTING OFF FOR CLEANI NG .....................................................................................................................................103
CLEANI NG AND CARE O F THE BLADE HOLDER ..................................................................................................................103
EMPTYING THE DEF ROSTI NG AND COLD D LIQUIDS .........................................................................................................104
EXCHANGE THE VACUT OM E FILT ER BAG ........................................................................................................................105
ADJUSTMENT OF THE MOV ABLE SPECIMEN HEAD JAW ....................................................................................................107
TRANSPORTATION OF THE INSTRUM ENT.........................................................................................................................108
Conditions for the Transportation of the Instrument.....................................................................................108
APPEN DICES ...............................................................................................................................................................110
APPENDIX A - SPARES AND ACCE SS ORI ES .......................................................................................................................110
APPENDIX B - RECOMMENDED DIS INFECTIO N PROCE DURE ..............................................................................................112
APPENDIX C - COLD DISINFECTION CERTIFICATE .............................................................................................................113
INDEX ..........................................................................................................................................................................114
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Chapter 1 - Safety Information
Thermo Scientific instruments are designed for convenient and reliable service; however, improper use or
handling by a user may damage the instrument. Protection might be impaired if the equipment is used in
a manner not specified by the manufacturer. Correct maintenance procedures are essential for consistent
performance. It is recommended that users secure a maintenance contract with our service department
acting on behalf of Thermo Shandon Limited.
Any problems and queries should be referred to your Thermo Fisher Scientific service department.
The following sections contain important information for the safe setup and use of the
instrument, and should be read and understood by the user before using the instrument.
General Safety
This instrument, as supplied, conforms to IEC 61010-1 and IEC 61010-2-
101; however, the
addition of chemicals introduces potential hazards. Good Laboratory Practice must be
employed and consideration must be given to the potential for hazard when dealing with
these chemicals.
Do not use the instrument in close proximity to strong electromagnetic radiation, as these
may interfere with the proper operation. The electromagnetic environment should be
evaluated prior to operation of the device.
Good Laboratory Practice must be used when handling tissue samples to prevent cross
contamination and infection. The user should complete a risk assessment to determine any
potential hazards related to tissue handling.
Do not introduce any source of ignition into, or near, the instrument once it has been loaded
with reagents.
Do not remove any panels or access covers, unless specifically instructed to do so. The
instrument does not have any user serviceable parts. Potentially lethal voltages are present
inside the instrument.
The instrument must be properly connected to a good earth (ground) via the Mains input
supply and positioned such that it is possible to interrupt the Mains supply at the source by
removing the plug from the socket.
Use only factory approved accessories or replacement parts within the instrument.
Only use reagents recommended in the operator guide.
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Chemical Safety
The introduction of chemicals creates potential hazards. Thermo Fisher Scientific has adopted the
following position with regard to the subject of volatile chemicals used in laboratories:
• Customers using non-specified chemicals in the instrument, do so at their own
risk.
• All chemicals recommended by Thermo Fisher Scientific have auto-ignition
temperatures considerably above any surface temperatures that can be reached
during a single fault failure on the instrument.
• The instrument contains no source o
f ignition in any areas of the instrument where
chemicals are stored, or likely to leak into, in a single fault condition.
•
The operator is fully aware of the contents of the specification documents detailing
the properties of the chemicals they are using.
• The operator has carried out any legally required assessment of chemicals used and
is using Good Laboratory Practice.
Environment
This instrument complies with the European Union's Waste Electrical and Electronic Equipment
(WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific on behalf of Thermo Shandon Limited has contracts with one or more recycling
/ disposal companies in each EU Member State, and this product and packaging should be disposed of or
recycled through them. For further information contact your Thermo Fisher Scientific service
representative.
Thermo Scientific CryoStar NX70 Operator Guide
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Warranty Statement
Thermo Shandon Limited as part of Thermo Fisher Scientific is proud of their quality, reliability and of
our after-sales service. We continuously strive to improve our service to our customers.
Please ask your distributor or Thermo Fisher Scientific representative about service contracts which can
help maintain your instrument in an optimal operating condition.
Warranty provisions necessarily vary to comply with differences in national and regional legislation.
Specific details can be found in the delivery documentation or from your dealer or representative.
Please note that your warranty may be invalidated if:
• This instrument is modified in any way, or not used as intended by The r mo Shandon L i mited.
• Accessories and reagents which have not been approved by Thermo Fisher Scientific are used.
• The instrument is not operated or maintained in accordance with instructions.
Thermo Scientific CryoStar NX70 Operator Guide
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Chapter 2 - Introduction
Intended Use
The Thermo Scientific cryostat NX70 is an in vitro diagnostic device, designed to take
precision sections of tissue specimens including the sectioning of paraffin embedded
samples in medical, pharmaceutical laboratories as necessary preparation for their
examination. Only qualified and trained laboratory personnel may operate the NX70.
The instrument may only be operated within the scope of its intended use as described above and as per
the instructions given in this manual.
Any other use of this instrument is considered as improper operation.
Instrument Features
The CryoStar NX70 is a precision-engineered, ergonomically-designed, high-performance cryostat in
modular design with a stainless steel cooling chamber. Some of its features are:
• Knife / Blade Holder cooling.
• Specimen cooling.
• Ergonomic height adjustment with integrated ergonomic casing.
• Colour touch-screen user interface and joystick control.
• Motorized cutting with variable speed control (from 0 to 256 mm/s).
• Optional Cold D function consisting of an internal fumigation unit that applies Sanosil®
(disinfection medium) into the user-accessible, operating areas of the cryochamber.
• Optional Vacutome function.
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Technical Specifications
Mechanical Specifications
Depth 100 cm (39.4 in)
Width 75.5 cm (29.7 in)
Height Range Adjustable range 82 - 112 cm (32.3 - 44.0 in)
Maximum Weight 200 kg (440 lb)
Electrical Specifications
Power Requirements
Dedicated line recommended.
100V, 50/60Hz, 10A, +/-10%, 110-120V, 60Hz, 10A, +/-10%
220-230V, 50/60Hz, 5A, +/-10%
Environmental Specifications
For Indoor Use Only
Temperature (Operating
Limits)
+5°C to +35°C (+41°F to +95°F)
Temperature
(Recommended
Operation)
+15°C to +30°C (+59°F to +86°F)
Note:
Performance may deteriorate when operated outside of this
range.
Temperature
(Transport and Storage)
-20°C to +40°C (-4°F to 122°F)
+70°C (158°F) for short exposure
Relative Humidity Max. 80% RH up to 31°C
Decreasing linearly to 50% RH at 40°C
Altitude Up to 2000 m (6,500 ft)
Pollution Degre e 2
Over Voltage Category II
Floor Loading
Requirements
300 kg/m² (660 lb/ft²)
Protection Class I
Sound Emission <45 dB (A) - measured with 1m distance to the instrument.
Refrigerant R404a, filling amount 400g
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Cooling Specifications
Specimen Temperature
Control
-50°C to +10°C (-58°F to +50°F) at +20°C room Temp. (decrease
0,7°C/1°C room temp. increase)
Blade Temperature
Control
-35°C to -5°C (-31°F to +23°F) at 20°C room Temp (decrease 0.5°C
71°C room temp. increase)
Cryobar Cooling Max. -60°C (-76°F) at -35°C blade temperature
Defrosting Options Programmed / Immediate
Microtome and Sectioning Specifications
Section Thickness Range 0.5 – 500 µm
Fine Section Thickness
Range
0.5 - 100 µm
Fine Section Thickness
Resolution
0.5 – 2 µm in 0.5 µm steps
2 µm – 10 µm in 1 µm steps
10 µm – 20 µm in 2 µm steps
20 µm – 50 µm in 5 µm steps
50 µm – 100 µm in 10 µm steps
Trimming Thickness
Range
5 - 500 µm
Trimming Thickness
Resolution
5 – 10 µm in 5 µm steps
10 µm – 100 µm in 10 µm steps
100 µm – 200 µm in 20 µm steps
200 µm – 500 µm in 50 µm steps
Specimen Retraction
(Return Travel)
20 µm
Vertical Specimen Stroke 64 mm
Horizontal Specimen
Movement
48 mm
Specimen Approach Manual (variable speed by joystick control), maximum speed: 3mm/s
Chuck Size 30, 40 mm (additional special sizes are available)
Specimen Orientation x - and y - axes universal 7° with zero positioning; z - axis up to 360°
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Coarse Feed Motorized
Cutting Drive Motorized, electronically controlled and manual via handwheel
Cutting Window Manually adjusted to specimen size
Operating Modes Interval, single, multi- and continuous-stroke
Cutting Speed 0 - 256 mm/s (0-120 1/min)
Clearance Angle
Adjustment
Disposable blade carrier 8- 16°
Vacutome Filter approximately 500 ml
Cold D Specification
Reservoir Volume 1000 ml
Recommended Medium Sanosil® S010 (5% H2O2)
Default Settings
Counter Mode Speed V = 0
Multi Counter 2
Operating Mode Continuous
Retraction ON
Language English
Fine 0.5 µm
Trim 5 µm
Joystick Calibration Calibrated
Chamber Light 100%
Screen Brightness 100%
Brake ON
Stepper Motor Initialization Yes
Blade Temperature -15°C
Specimen Temperature -15°C
Cutting Window 0 mm (OFF)
Favorites (Program 1, Program 2,
Program 3)
(-15°C/-15°C), (-20°C/-20°C), (-25°C/-25°C)
Cryobar OFF
Vacutome OFF
Disinfection OFF
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Disinfection, Cycle (Automatic) ON (03:00 Night)
Defrost Cycle (Automatic) ON (00:00 Night)
Standby Mode 1h (After 1 hour, the Cryostat enters the Standby Mode
automatically). Kept temperature: -15°C.
Sleep Mode 3h (After 3 hours, the Cryostat enters the Sleep Mode
automatically). Kept temperature: -10°C. Power savings:
Approximately 30%.
Start Time 06:00 a.m.
Section Counter 0
Section Sum 0
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Chapter 3 - Instrument Setup
Unpacking and Repacking
Safety Precautions
The CryoStar NX70 is heavy and requires a minimum of two people to safely unpack and
manoeuvre it.
The CryoStar NX70 has a high centre of gravity and care should be taken when moving.
Do not tip the CryoStar NX70more than 30° from the upright position.
After moving the CryoStar NX70, wait at least 8 hours before switching on to allow the
refrigerant to settle - failure to do so may cause damage to the unit.
Note
The packing materials should be carefully stored for use in any future instrument shipment.
Unpacking Procedure
General Information:
• Ensure that these Instructions are retained for future use.
• The CryoStar NX70 is delivered on a pallet. The sides of the instrument are encased in cardboard
and cushioned with foam spacers inside the container. A wooden cover protects the top of the
instrument.
• In unpacking be aware of the weight (200 Kg/440 lbs) of the instrument.
Safe handling of the installation requires the involvement of two people
Handling
:
• Place the crate on a flat surface while ensuring that there are no objects underneath the pallet.
• Remove the wooden cover form the crate but retain it for later use as a ramp.
• Remove the top foam spacer from the crate.
• Remove the exterior cardboard container by opening the clips.
• Remove the remaining foam spacers and the accessories carton. Place all the accessories carefully
to one side.
• Remove the plastic cover from the instrument.
• Two wing-nuts are now visible at the front edges of the wooden pallet.
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• Using the wing-nuts affix the wooden top of the crate to the pallet to serve as an unloading ramp
for the instrument.
• The instrument can be moved on its castors. However, ensure that it does not roll off the pallet
inadvertently.
• Screw in the two bails (part of the standard accessories) into the respective holes in the foot of the
cryostat.
• Mount the transport belts (part of the standard accessories) with the bails.
To move the instrument from the pallet stand BEHIND (NOT in front) of the
instrument.
• Carefully roll the instrument off the pallet and on to the floor.
• Move the instrument to the desired location while leaving space on the left- and right-hand.
• Keep all packing and transportation materials together in case of future need.
• In the future, if the instrument is transported by forklift, truck, train, ship or aircraft it must be
packed in the original shipping container with all transportation locks in place.
Repacking the Instrument
• To repack the instrument, follow the above instructions in reverse order.
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Location & Setup
When deciding where to locate the CryoStar NX70, the following items must be taken into
consideration:
• Approximately 10 cm (4 in) must be allowed around the cooling system to allow free air flow.
• Approximately 10 cm (4 in) must be allowed between the wall and the rear panel.
• The vents on the sides of the rear panel must be unobstructed at all times.
• The mains power socket must be accessible at all times.
• The location must be free from draughts, open doors or air conditioning systems.
• The location must not expose the cryochamber to direct sunlight.
• Enough room for height adjustment.
Note
These measures are intended to reduce the build-up of frost and maintain consistent cooling temperatures
resulting in more favourable working conditions. Locations which have high humidity and / or high
ambient temperatures can reduce the performance of the instrument.
Once the CryoStar NX70 has been located, ensure the fixing bolts shown are tightened to
prevent the unit from moving during operation.
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Identification of Parts
Overview
1
Cryochamber
6
Connections Panel
2
Cryochamber Window
7
Handwheel
3
Touch Screen
8
Handwheel Mechanical Brake
4
Cold D Reservoir Cover
9
Water Drain Reservoir Cover
5
Emergency Stop Button
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Connections Panel
10 Power Switch 13 X1 – RJ45 port (for Service purposes only)
11 Power Socket 14 X2 – USB port (for service purposes only)
12 Rating and Serial Number Label 15 X3 – Foot Pedal Connector
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Cryochamber
16 Cryobar and Cooled Storage Area 23
Cutting Speed Control
17 Trim Button 24
Motorized Cutting
Start / Stop Button
18 Joystick 25
Handwheel Electronic Brake Button
19 Specimen Head 26
Vacutome Filter Cover
20 Blade Holder with Anti-Roll Plate 27
Vacutome Filter Cover Release Knob
21 Vacutome 28
Section Waste Tray
22 Mode Selection Button
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Blade Holder
29
Lateral Adjustment Lever
33
Blade Holder Base
30
Anti
-Roll Plate
34
Anti-Roll Plate Adjustment
31
Blade Clamping Lever
35
Clearance Angle Adjusting Screw
32
Top Stage (Angle Adjustment)
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Specimen Head NX70
36 Y-axis Fine Adjustment Knob 39 Specimen Head Clamping Lever
37 Specimen Chuck Release Lever 40 Upper Specimen Chuck Jaw (Static)
38 X-Axis Fine Adjustment Knob 41 Lower Specimen Chuck Jaw (Moving)
Thermo Scientific CryoStar NX70 Operator Guide
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Set Up
Before sectioning, the blade holder and the Cryochamber should be at a stable temperature close to the
desired cutting temperature. These temperatures should be selected to suit the consistency of the
specimen.
All tools which come into contact with the sections or which are used to manipulate the specimen must
also be cooled to prevent the tissue from sticking to them. Therefore all accessories should be stored on
the shelves or in the brush tray in the Cryochamber. The exceptions to this rule are the specimen chucks
which should be kept at room temperature.
The heated sliding window should be closed during periods of inactivity and while preparing specimens
to avoid warm air getting into the chamber. This will result in more favourable working conditions and a
lowe r frost build-up.
Ensure that the Vacutome filter is properly inserted in the filter unit and is suitably maintained - see
Cleaning and Maintenance.
Fitting or Exchanging the Anti Roll Plate
• The Anti Roll Plate is held in place by two black screws.
• Ensure that the gap clearance side of the Anti-Roll Plate is facing inwards.
• Position the Anti Roll Plate in the
holder, gently pushing the glass to the
bottom of the holder.
• Tighten the black screws.
Note
There are two usable edges on the Anti Roll Plate.
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Fitting or Exchanging Low Profile Blades
Microtome blades are extremely sharp and can cause severe injuries- always use good
laboratory practice when handling them.
Always cover the blade with the knife / blade guard and activate the electronic and
mechanical brakes prior to making modifications to the blade/knife and specimen or
changing the specimen.
The blade holder should be fitted with low profile blades (80mm x 8mm; 35° facet angle) as standard.
To fit or exchange low profile blades:
• Use only the front handle to
move the anti-roll plate to the
side.
• Pull the blade clamping lever
towards the front of the
instrument to release the
clamping plate.
• Carefully slide a low profile blade
into the middle of the slot,
ensuring that the blade is
equidistant from the ends of the
slot.
• Push the blade clamping lever
towards the back of the
instrument to clamp the blade in
position.
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Removing Used Blades with the Magnetic Blade Tool
The magnetic blade tool allows the user to remove used blades from the blade holder in a safe way.
Remove the used blade with the magnetic blade tool:
• Use only the front handle to
move the anti-roll plate to the
side.
• Pull the blade clamping lever
towards the front of the
instrument to release the
clamping plate.
• Move the magnetic blade tool
over the blade.
• Carefully lift the blade out of the blade holder and dispose of it in accordance with laboratory
regulations.
Thermo Scientific CryoStar NX70 Operator Guide
29
Blade Positioning and Orientation
Always cover the blade with the knife/blade guard and activate the electronic and
mechanical brakes prior to making modifications to the blade/knife!
If the cutting area of the blade is no longer usable, the upper part of the blade holder can be moved
without removing the blade.
To move the upper part of the blade holder:
• Loosen the lateral adjustment lever.
• The upper part of the blade holder should now be free to move left and right.
• Move it either to the left or to the
right until the desired area of the
blade is directly below the specimen
head.
• Move the lateral adjustment lever
forward to secure the upper part of
the blade holder in the new
position.
Thermo Scientific CryoStar NX70 Operator Guide
30
Adjusting the Cutting Angle
The cutting angle can be adjusted. The recommended cutting angle is approximately 10° for Thermo
Scientific low profile blades.
Note
The factory pre-set cutting angle is 10°.
• Loosen the clearance angle
adjustment screw on the lower left
site of the blade holder.
• Gently push both sides of the blade
holder base to select the desired
angle.
The surface of the blade holder base might be cold. Use gloves if necessary
Note
The clearance angle can be read on the scale on the left side on the upper part of the blade holder.
Note
10° is indicated by the longer increment in the middle of the scale.
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