Thermo Scientific COL015088 User Manual

Nunc Cell Factory equipment

Develop an automated, consistent, and proven
commercial-scale process for adherent cell culture

Ideal applications

• Large-scale cell culture

• Cell therapeutics

• Vaccine production

The integrated solution for
Nunc Cell Factory systems

Take your Nunc Cell Factory systems to the next level

Thermo Scientifi c™ Nunc™ Cell Factory™ equipment delivers a solution for quick and easy
scale-up of your existing Thermo Scientifi c™ Nunc™ Cell Factory™ systems. Nunc Cell Factory
equipment automates the fi lling, emptying, harvesting, and trypsinization steps of adherent cell
culture. Realize process consistency, optimization, and standardization when scaling up and
manipulating multiple Nunc Cell Factory systems simultaneously with this trusted equipment. All
equipment is compliant with cGMP.

Automated solutions

• Thermo Scientifi c™ Nunc™ Automated Cell Factory
Manipulator—fully programmable automation for

fi lling and emptying twelve 10-layer or four 40-layer
Nunc Cell Factory systems; designed for use in clean
room environments

™

• Thermo Scientifi c

mobile solution for incubation of forty-eight 10-layer
Nunc Cell Factory systems or sixteen 40-layer Nunc Cell
Factory systems: available with and without CO2 control

• Thermo Scientifi c
System—automated solution for the detachment of cells

from Nunc Cell Factory systems

Nunc™ Cell Factory™ Incubator—

™

Nunc™ Cell Factory™ Shaker

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Accessories

• Thermo Scientifi c
carts—each rack can hold up to twelve 10-layer or

four 40-layer Nunc Cell Factory systems; designed to
integrate perfectly with all Nunc Cell Factory equipment

• Thermo Scientifi c
manipulator systems—designed for the safe

and e cient handling of 10-layer and 40-layer
Nunc Cell Factory systems

• Additional Nunc Cell Factory systems and connectivity
accessories are available

™

Nunc™ Cell Factory™ racks and

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Nunc™ Cell Factory™ hand

• Other biological products

Production scale-up solutions

Utilizing the Nunc Cell Factory equipment portfolio

The following steps highlight a cell expansion process and
where to use the Nunc Cell Factory equipment:

1. Seed cells into four 40-layer Nunc Cell Factory systems
using up to 8 L cell culture medium per 40-layer Nunc
Cell Factory system

2. Incubate overnight at 37°C

3. Tr y p sinize

– Empty 40-layer Nunc Cell Factory systems of cell

culture medium using the Nunc Automated Cell
Factory Manipulator

Nunc Cell Factory
Incubator

– Wash with up to 1.6 L PBS, then remove PBS using

Nunc Automated Cell Factory Manipulator

– Add/cover cells with 600 mL of pre-warmed 0.25%

trypsin-EDTA using Nunc Automated Cell Factory
Manipulator

– Incubate at room temperature or 37

°C

until 90% cell detachment is achieved

4. If cells are especially adherent, shake using the
Nunc Cell Factory Shaker System

5. Add 1 L stopping solution (cell culture medium)
and harvest suspension

Nunc Cell Factory
racks and carts

Nunc Cell Factory
Shaker System

2. Incubating

1. Cell seeding

3. Trypsinizing

4. Shaking*

Nunc Automated
Cell Factory Manipulator

5. Harvesting

* Shaking can be done in the Nunc Automated Cell F actory Manipulator depending on cell adherence.