Thermo Scientific COL015088 User Manual

Nunc Cell Factory equipment

Develop an automated, consistent, and proven
commercial-scale process for adherent cell culture

Ideal applications

• Large-scale cell culture

• Cell therapeutics

• Vaccine production

The integrated solution for
Nunc Cell Factory systems

Take your Nunc Cell Factory systems to the next level

Thermo Scientifi c™ Nunc™ Cell Factory™ equipment delivers a solution for quick and easy
scale-up of your existing Thermo Scientifi c™ Nunc™ Cell Factory™ systems. Nunc Cell Factory
equipment automates the fi lling, emptying, harvesting, and trypsinization steps of adherent cell
culture. Realize process consistency, optimization, and standardization when scaling up and
manipulating multiple Nunc Cell Factory systems simultaneously with this trusted equipment. All
equipment is compliant with cGMP.

Automated solutions

• Thermo Scientifi c™ Nunc™ Automated Cell Factory
Manipulator—fully programmable automation for

fi lling and emptying twelve 10-layer or four 40-layer
Nunc Cell Factory systems; designed for use in clean
room environments

™

• Thermo Scientifi c

mobile solution for incubation of forty-eight 10-layer
Nunc Cell Factory systems or sixteen 40-layer Nunc Cell
Factory systems: available with and without CO2 control

• Thermo Scientifi c
System—automated solution for the detachment of cells

from Nunc Cell Factory systems

Nunc™ Cell Factory™ Incubator—

™

Nunc™ Cell Factory™ Shaker

™

Accessories

• Thermo Scientifi c
carts—each rack can hold up to twelve 10-layer or

four 40-layer Nunc Cell Factory systems; designed to
integrate perfectly with all Nunc Cell Factory equipment

• Thermo Scientifi c
manipulator systems—designed for the safe

and e cient handling of 10-layer and 40-layer
Nunc Cell Factory systems

• Additional Nunc Cell Factory systems and connectivity
accessories are available

™

Nunc™ Cell Factory™ racks and

™

Nunc™ Cell Factory™ hand

• Other biological products

Production scale-up solutions

Utilizing the Nunc Cell Factory equipment portfolio

The following steps highlight a cell expansion process and
where to use the Nunc Cell Factory equipment:

1. Seed cells into four 40-layer Nunc Cell Factory systems
using up to 8 L cell culture medium per 40-layer Nunc
Cell Factory system

2. Incubate overnight at 37°C

3. Tr y p sinize

– Empty 40-layer Nunc Cell Factory systems of cell

culture medium using the Nunc Automated Cell
Factory Manipulator

Nunc Cell Factory
Incubator

– Wash with up to 1.6 L PBS, then remove PBS using

Nunc Automated Cell Factory Manipulator

– Add/cover cells with 600 mL of pre-warmed 0.25%

trypsin-EDTA using Nunc Automated Cell Factory
Manipulator

– Incubate at room temperature or 37

°C

until 90% cell detachment is achieved

4. If cells are especially adherent, shake using the
Nunc Cell Factory Shaker System

5. Add 1 L stopping solution (cell culture medium)
and harvest suspension

Nunc Cell Factory
racks and carts

Nunc Cell Factory
Shaker System

2. Incubating

1. Cell seeding

3. Trypsinizing

4. Shaking*

Nunc Automated
Cell Factory Manipulator

5. Harvesting

* Shaking can be done in the Nunc Automated Cell F actory Manipulator depending on cell adherence.

Nunc Automated Cell Factory Manipulator

Increase processing speed and eliminate manual handling

The Nunc Automated Cell Factory Manipulator is a
high-quality, fully programmable system that automates the
handling of Nunc Cell Factory systems. Keep your proven
adherent cell culture practices and simplify your scale-up
to achieve consistent and reliable results. Designed for use
in clean room environments and compatible with closed
system workflows, all functions can be activated by the
touch of a button.

Features

• Can handle twelve 10-layer or four 40-layer standard
Nunc Cell Factory systems (twelve 13-layer or four
52-layer Thermo Scientific™ Nunc™ High Density Cell
Factory™ systems)*

• Standard safety light curtain

• All visible components made of 316L stainless steel
for easy cleaning

• Comes with a total of 29 programs: 11 fixed basic
programs and 18 preinstalled, modifiable wash programs

• Programs to help automate filling, washing, emptying,
trypsinizing, and harvesting steps of Nunc Cell
Factory systems

D

C

E

Required accessories

A. Nunc Cell Factory systems (ten 40-layer Standard

Nunc Cell Factory systems or thirteen 52-layer High
Density Nunc Cell Factory systems)

B. Nunc Cell Factory racks and carts

C. Tube sets and filter assemblies

D. Peristaltic pump

™

E. Thermo Scientific

Nalgene™ Carboys, BPEs,

or other containers

A

B

• Optional Process Field Bus (PROFIBUS) data interface

• Can be part of a closed system workflow

• Compatible with many “sensitive” cell lines

Consumables customization

Nunc Cell Factory systems can be configured with tubing
sets and manifolds to enable a closed system workflow
that works seamlessly with the Nunc Automated Cell
Factory Manipulator. These tubing sets are customized to
your specific needs through our configuration service.

* A simple component adjustment is needed for use with Nunc High Density Cell Factory systems.
** Other voltages on request.

Nunc Automated Cell Factory Manipulator

Specifications

Footprint (W x D x H)

Construction 1.4404 (AISI 316L) stainless steel

Unit weight 1,050 kg (2,314.9 lb)

Load capacity >200 kg (>440.9 lb)

Rated voltage 208–400 V, triple phase, 50/60 Hz**

Current draw 6.5–15.5 A (each phase)

Connected load 4.5 – 5.6 kVA

Declaration of conformity

Machinery directive 20 0 6/42 / EC

Electromagnetic
compatibility
directive

206.8 x 179 x 194.4 cm (max)

81.4 x 70.47 x 76.54 in. (max)

2014/30/EC

Nunc Cell Factory Incubator

An incubation unit designed for Nunc Cell Factory systems

Achieve process consistency with Nunc Cell Factory
Incubators. Mitigate the risk of spillage from moving full cell
factories. The convenient fl oor-level height of the shelves
makes it easy to load and unload carts. This equipment
is designed to seamlessly accommodate four racks of
10-layer or 40-layer Nunc Cell Factory systems.

Features

• Can handle forty-eight 10-layer or sixteen 40-layer
standard Nunc Cell Factory systems (forty-eight
13-layer or sixteen 52-layer Nunc High Density
Cell Factory systems)

• Available with and without CO

• Optional CO

• Equipped with warning and alarm systems

• Temperature control from 7°C to 50°C

• Incubator cabinet and interior made of stainless-steel
grade 316L for easy cleaning

• Can be part of a closed system workfl ow

sensor with infrared technology

2

control (0–20% CO2)

2

Nunc Cell Factory Incubator

Specifi cations

Footprint (W x D x H)

Construction 1.4404 (AISI 316L) stainless steel

Unit weight 600 kg (1,322.8 lb)

Load capacity 600 kg (1,322.8 lb)

Rated voltage 208–240 V, single phase, 50/60 Hz

Rated power 1.25 kVA at 230 V

Current draw 5.4 A at 230 V

Range 7°C above ambient to 50°C

Uniformity ±0.6°C at 37°C

Power consumption 320 Wh/h at 37°C operation

Declaration of conformity

Machinery directive 2 014/ 3 5/EC

Electromagnetic
compatibility directive

186 x 118.5 x 148.9 cm

73.23 x 46.65 x 58.62 in.

2014/30/EC

Nunc Cell Factory Shaker System

Uniformly detach especially adherent cells

The Nunc Cell Factory Shaker System o ers a high-quality,
automated solution for the consistent detachment of
especially adherent cells from Nunc Cell Factory systems. It
eliminates the need for shaking and pounding, supporting
workfl ow dependability and e ciency.

Features

• Can handle twelve 10-layer or four 40-layer standard
Nunc Cell Factory systems (twelve 13-layer or four
52-layer Nunc High Density Cell Factory systems)

• Performs a continuous, horizontal accelerated and
decelerated movement

• Programmable shaking time

• Shaking frequency can be adjusted between 0 and 2 Hz

• All visible components made of 304L stainless steel for
easy cleaning

• Standard safety light curtain

• Can be part of a closed system workfl ow

Nunc Cell Factory Shaker System

Specifi cations

Footprint (W x D x H)

Construction 1.4301 (AISI 304L) stainless steel

Unit weight 650 kg (1,433.0 lb)

Load capacity >125 kg (275.6 lb)

Rated voltage 208–240 V, single phase, 50/60 Hz

Rated power 1 kVA

Current draw 4.3 A

Connected load 1.0 k VA

Declaration of conformity

Machinery directive 2006/42/EC

Electromagnetic
compatibility directive

170.6 x 59 x 128.2 cm

67.2 x 23.2 x 50.5 in.

2104/30/EC

Nunc Cell Factory racks and carts

Integration with all Nunc Cell Factory equipment

Easily store and transport Nunc Cell Factory systems safely
and eciently using Nunc Cell Factory racks and carts.
Each rack can hold up to four Nunc Cell Factory 40-layer
systems or twelve Nunc Cell Factory 10-layer systems.
Racks and carts are designed to integrate perfectly with all
Nunc Cell Factory equipment, adding consistency to and
streamlining your process.

Features

• Can handle twelve 10-layer or four 40-layer standard
Nunc Cell Factory systems (twelve 13-layer or four
52-layer Nunc High Density Cell Factory systems)

• Optional tubing clamp for 13.5 mm or 16 mm OD tubing

• Designed for use in clean room environments

• Side-loading design for easy loading and unloading

• Made of 316L stainless steel for easy cleaning

• Movable rack designed to come o from cart and interact
seamlessly with other Nunc Cell Factory equipment

• Nunc Cell Factory 40-layer carts can accommodate
other layered adherent cell culture systems

• Nunc Cell Factory 10-layer carts can be customized
to accommodate other layered adherent cell
culture systems

Nunc Cell Factory racks and carts

Specifications

Footprint
(W x D x H)

Construction 1.4404 (AISI 316L) stainless steel

Unit weight 65 kg (143.3 lb)

Load capacity

Integrates with:

• Nunc Cell Factory Incubator

• Nunc Automated Cell Factory Manipulator

• Nunc Cell Factory Shaker System

• Nunc Cell Factory hand manipulators

• Fluid transfer assemblies

103.5 x 36 x 96.6 cm

40.7 x 14.2 x 38 in.

60 kg (twelve 10-layer Nunc Cell
Factory systems)
80 kg (four 10-layer Nunc Cell
Factory systems)

Nunc Cell Factory hand
manipulator systems

Manipulate Nunc Cell Factory systems with ease

Nunc Cell Factory hand manipulators are designed for
the safe and e cient handling of individual 10-layer and
40-layer Nunc Cell Factory systems. Constructed of quality
stainless steel, they are ideal for clean room environments.
The Thermo Scientifi c™ Nunc™ Cell Factory™ 40-layer hand
manipulator is mobile and includes a foot brake for safe
placement. The Thermo Scientifi c™ Nunc™ Cell Factory
10-layer hand manipulator is compact and ideal for use
within in a biosafety cabinet.

Nunc Cell Factory hand manipulator system

Specifi cations

™

10-layer

Footprint (W x D x H)

Construction 1.4301 (AISI 304L) stainless steel

Unit weight 9.5 kg (20.9 lb)

40-layer

Footprint (W x D x H)

Construction 1.4301 (AISI 304L) stainless steel

Unit weight 27 kg (59.5 lb)

30.3 x 55.7 x 36.6 cm

11.9 x 21.9 x 14.4 in.

76.5 x 67 x 135.2 cm (max)

30.1 x 26.4 x 53.2 in. (max)

40-layer Nunc Cell Factory hand manipulator system

10-layer Nunc Cell Factory hand manipulator system

Nunc Cell Factory equipment applications

Vaccine production

This system can play an integral
part in the passaging, inoculation,
and harvest steps of the vaccine
production workflow.

Cell therapy

Nunc Cell Factory equipment can be
used in the cell line expansion
of mesenchymal stem cells (MSCs).

Gene therapy and
virus production

This system can be used in the
passaging, inoculation, and harvesting
steps of virus production from lentiviral
and AAV cell lines.

Ordering information

Description Cat. No.

Nunc Automated Cell Factory Manipulator

Nunc Automated Cell Factory Manipulator Gen 10, with safety light curtain and membrane foil keypad,
3N/PE, 400 V, 50/60 Hz

Nunc Automated Cell Factory Manipulator Gen 10, with safety light curtain and membrane foil keypad,
3N/PE, 208 V, 50/60 Hz

Nunc Cell Factory Incubator

Nunc Cell Factory Incubator, Gen 5, 208–240 V, 50/60 Hz, single phase 12010 0

Nunc Cell Factory Incubator, with CO

Nunc Cell Factory Shaker System

Nunc Cell Factory Shaker, Gen 2, 208–240 V, 50/60 Hz, single phase 132849

control, Gen 5, 208–240 V, 50/60 Hz, single phase 120300

2

120 288

120 4 05

Nunc Cell Factory Shaker, Gen 3, 208–240 V, 50/60 Hz, with data logging 13285 4

Nunc Cell Factory racks and carts

Side Loading Rack with Cart for 40-Layer Nunc Cell Factory systems and 52-Layer
Nunc High Density Cell Factory systems

Side Loading Rack with Cart for 10-Layer Nunc Cell Factory systems and 13-Layer
Nunc High Density Cell Factory systems

Retrofit Clamping Kit for Racks for Use with Shaker 120 289

Nunc Cell Factory hand manipulator system

Nunc 10-Layer Cell Factory Hand Manipulator System 132752

Nunc 40-Layer Cell Factory Hand Manipulator System 176 9 5 3

14050 3

14050 4

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For Research Use or Further Manufacturing. Not for diagnostic u se or direct administration into humans or animals.
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subsidiaries unless otherwise specified. COL015088 0421