Thermo Scientific AQ4500 Instruction Manual
Thermo Scientific Orion
AQUAfast AQ4500 Turbidimeter
User Guide
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Table of Contents
Chapter 1
Chapter 2
Chapter 3
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Principle of Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Display .
Keypad .
Meter Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Battery Installation .
Vial Cover .
RS232 Connection .
Setup Menu
Setup Functions .
Cal Chek . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Clock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
S
P
A
D
B
S
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
et Baud . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
rint. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
uto Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
igits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
attery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
elf Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Chapter 4
Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Preparing and Filling the Calibration Standard Vials . . . . . . . . . . . . . . 14
Calibration Check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Preparation of Low Turbidity Water .
Initial Calibration for EPA 180.1, ISO-NEPH or IR Ratio Modes . . . 17
aily Calibration Check for EPA 180.1, ISO-NEPH
D
and IR Ratio Modes .
Calibration of ISO-ABSB Mode .
Calibration of White %T Mode .
Calibration of IR %T Mode .
Refilling the Calibration Standard Vials with Fresh Standard. . . . . . . . 19
Preparation of Primary Calibration Standards
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
. . . . . . . . . . . . . . . . . . . . . . . . . 16
. . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
. . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
. . . . . . . . . . . . . . . . . . . 20
I
Chapter 5
Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Turbidity Sample Measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
easurement of Low Level Turbidity Samples (< 1 NTU) . . . . . . . . 22
M
easurement Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
M
EPA Interim Approval Letter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Chapter 6
Chapter 7
Chapter 8
Appendix A
Functions .
Log Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Average Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Use with Printers and Computers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Data Transmission Settings .
Customer Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Turbidimeter Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Turbidimeter Maintenance .
Vial Maintenance .
Operator Assistance Codes .
Assistance .
Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Declaration of Conformity .
Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
ungsten vs. LED Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
T
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
II Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
Chapter 1 Introduction
The AQUAfast AQ4500 turbidimeter is the most advanced portable
microprocessor based LED turbidimeter on the market today. The
AQ4500 turbidimeter operates on the nephelometric and ratiometric
principles of turbidity measur
turbidity measurement based on EPA 180.1 and ISO 7027 as well as an
infrared ratio (IR ratio) mode that gives results in accordance with EPA
GLI method 2
based on percent transmittance (%T), American Society of Brewing
Chemists (ASBC) units or E
The AQUAfast AQ4500 turbidimeter is the only advanced completely
waterpr
data points that can later be downloaded to a printer or computer.
The AQUAfast AQ4500 turbidimeter has received interim approval
fr
om the EPA for wastewater turbidity reporting when in the EPA180.1
mode and following Thermo Orion Method AQ 4500, Revision 5. See
the EPA Interim Approval Letter section of this user guide and visit
www.
thermo.com/waterapps for updated information on EPA approval.
Until final approval from the EPA for turbidity reporting, please contact
your state or local regulatory agency for their requirements for interim
oval methods. Refer to Appendix A for information on the benefits
appr
of a white LED light sour
. The AQ4500 also allows the user to make measurements
oof turbidimeter with a rating of IP67. The AQ4500 can log 100
ement. The AQ4500 turbidimeter allows
uropean Brewing Chemists (EBC) units.
ce for turbidity measurements.
Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
1
IR LED
Emitted Light
Light
Detector
90˚ Scattered
Light
Principle of
Operation
Figure 1
Pure Nephelometric
Turbidity
Measurement
without Color
Compensation
Figure 2
Color Compensation
in the Dual-beam
System with Two
Photo Detectors
Nephelometric turbidity is measured by determining the sidewardscattered light intensity. A light beam is passed through the flow cell and
the scattered light produced by the solid particles (turbidity) is detected
at a specific angle
. This measurement method ignores the light that passes
straight through the cell.
If the medium contains absorbent substances (such as colored
constituents), these can easily attenuate the light beam by factors ranging
om 2 to 10 and thus falsify the results. So it is absolutely necessary to
fr
eliminate the effect of the medium’s absorption in such cases. This is
achieved by using two light beams: a measurement beam and a reference
beam
. The turbidity is thus determined from their ratio.
2 Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
Display
Figure 3
AQ4500 Display
Item Number Description
1 Calibrate Icon lit while AQ4500 is in calibration mode
2 Setup Icon lit while AQ4500 is in setup mode
3 Measure Icon lit during measurement
4 Avg Icon lit while AQ4500 performs zero
5 Units Icons for units of measurement
6 ? Icon lit when AQ4500 prompts a question
7 Time Icon lit while timer is active
8 Log Icon lit if data is in AQ4500 log
9 Battery Icon lit when battery in AQ4500 is low
10 Print Icon lit during print function
11 188 Not used
12
13 Numeric display
Alphanumeric display
Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
3
Orion AQ4500
Keypad
Key Description
Turns the AQ4500 on or off
Initiates print mode or inputs number 0
Confirms an answer or inputs a decimal point
Allows selection of measurement units or inputs number 1
Initiates log mode or inputs number 2
Scrolls down, answers “NO” or inputs number 3
Initiates Average mode or inputs number 4
Displays Time/Date and Stopwatch or inputs number 5
Figure 4
AQ4500 Keypad
Scroll ups, answers “NO” or inputs number 6
Initiates Setup function or inputs number 7
Initiates a calibration or inputs number 8
Initiates a measurement or inputs number 9
4 Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
Chapter 2 Meter Setup
Battery
Installation
Figure 5
Battery Installation
The AQ4500 turbidimeter requires 4 AA alkaline or lithium batteries.
With 4 alkaline batteries, the expected life is 2,500 hours. With lithium
batteries the expected life is 10,000 hours.
To install batteries, carefully loosen the two captive screws on the bottom
of the batter
Figure 5.
Replace battery cover and tighten captive screws.
y cover. Remove the cover and insert batteries as shown in
The AQ4500 has an auto-shutoff feature to conserve battery life. The
auto-shutoff will turn the turbidimeter off if no keys have been pressed
for appr
When the battery gets low “battery” will be displayed. Also at power-up
an indication of battery life will be displayed.
Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
oximately 20 minutes.
5
Vial Cover
Figure 6
The vial cover serves two functions; first, it keeps the optical well
covered so water, dirt and dust do not enter the well. Second, it covers
the vial during measurement to prevent stray light from affecting the
measur
AQ4500 is waterproof to IP67 standards.
The vial cover can be used in two ways. First for measurement when the
waterproof integrity of the meter is important, place the vial cover over
the vial and screw down as shown in Figure 6. To remove, unscrew and
lift up.
ement. When the vial cover is installed and screwed down the
Figure 7
If using turbidimeter in a lab where waterproof integrity is not a factor,
the co
ver can just be placed down over the vial. To remove vial cover, lift
cover straight up as shown in Figure 7.
6 Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
Figure 8
During measurement it is important to place the vial into the AQ4500
aligning the white triangle (
turbidimeter. See Figure 8 aligning vial with tab on turbidimeter.
s) on the vial with the tab on the
Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
7
RS232
Connection
Figure 9
AQ4500 RS232
Cable Connection
The AQ4500 has a bidirectional RS232 port located on the underside of
the turbidimeter. See Figure 9.
computer, use the optional RS232 cable, Cat. No. AQ4CBL. See the Use
With Printers and Computers section
To connect the AQ4500 to a printer or
.
8 Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
Chapter 3 Setup Menu
setup
Setup
Functions
Cal Chek
The setup key allows the user to access all the user selectable functions in
the AQ4500 turbidimeter.
Cal Chek
Clock
Set Baud
Print
Auto Print
Digits
Battery
Self test
Allows the user to confirm the time and date of the last
calibration for the measurement mode selected.
Allows the user to set the date and time.
Allows the user to set the baud rate for the meter when using
it with a printer or computer.
Allows the user to set the print out format.
Allows the user to set the turbidimeter to automatically print
each measurement.
Allows the user to select the number of significant digits to be
displayed.
Allows the user to view remaining battery life.
Allows the user to initiate a self test to verify meter operation.
Allows the user to confirm the last calibration for the selected
measurement mode.
1. Press the
key.
2. Press the
3. Press the
or key until “CAL CHEK” is displayed.
key to accept.
4. The last calibration date and time will be displayed.
5. Press the
or key to return to the setup mode or press the
key to return to measurement mode.
Thermo Scientific Orion AQUAfast AQ4500 Turbidimeter User Guide
9
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