Thermo Scientific AI 3000, AS 3000, AS 3000 II Operating Manual
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AI 3000 / AS 3000
AI 3000 / AS 3000 II
3000 Series Automatic Sampling System
Operating Manual
P/N 31709392, Tenth Edition, March 2012
© 2012 Thermo Fisher Scientific Inc. All rights reserved.
AI 3000 II, AS 3000 II, TRACE 1300, TRACE 1310, TRACE GC Ultra, and FOCUS GC are trademarks of
rmo Fisher Scientific Inc., and its subsidiaries.
The
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do
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Release history: First edition, January 2002; Second Edition, May 2003; Third Edition, September 2003
o
urth Edition, April 2004; Fifth Edition, September 2004; Sixth Edition, May 2007; Seventh Edition,
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December 2007 (see the Note (*); Eighth Edition, April 2009; Ninth Edition, May 2010; Tenth Edition,
March 2012
(*) Note: The seventh edition of this manual considers the AI 3000/AS 3000 II automatic sampling system.
For Research Use Only. Not for use in diagnostic procedures.
AI 3000 / AS 3000 II Operating Manual, PN 31709392, Tenth Edition
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Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
r the instrument safety, reliability and performance only if:
fo
•installation
•re-calibration
•changes and repairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
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inent electromagnetic compatibility (EMC) and safety
Safety
This device complies with EN 61010-1:2001, EN 61010-2:2002 according to Low Voltage Directive 2006/95/EC.
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C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiv
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Rating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
The AI 3000/AS 3000 II Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Configurations of the 3000 Series Automatic Sampling System. . . . . . . . . . . . . . 5
AI 3000 Auto-injector Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
AS 3000 Auto-sampler Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Gemini Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 2 Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Turret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Injection Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Vial Capture Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Turret Movements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Washing and Waste Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Rear of the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Sample Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
8-Position Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
105-Position Sample Tray Assemble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Centering Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Centering Plate for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . 18
Centering Plate for TRACE GC Ultra and FOCUS GC. . . . . . . . . . . . . . . . 19
Status LED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual ix
Contents
Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Who Performs the Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
How to Lift and Carry the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Sampling System Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Sampling System Support for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . 23
Sampling System Support for TRACE GC Ultra. . . . . . . . . . . . . . . . . . . . . . 26
Sampling System Support for FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . 28
References for the Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Installing the Sampling System Support on the TRACE 1300/TRACE
1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Assembling of the Sampling System Support on the Front Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Assembling of the Sampling System Support on the Back Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Assembling of the Sampling System Support for the Gemini
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Assembling of the Sampling System Support When a TSQ Quantum
is Coupled with the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Installing the Sampling System Support on the TRACE GC Ultra . . . . . . . . . . 43
Installing the Spacer Inside the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Assembling of the Sampling System Support on the GC Right Side . . . . . . . 44
Assembling of the Sampling System Support on the GC Left Side. . . . . . . . . 44
Installing the Sampling System Support on the FOCUS GC. . . . . . . . . . . . . . . 46
Assembling of the Sampling System Support on the GC right side . . . . . . . . 46
Assembling of the Sampling System Support on FOCUS GC MS
Version. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Space Saving Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Installation of the AI 3000/AS 3000 II on the GC . . . . . . . . . . . . . . . . . . . . . . 49
Installation of the Sampling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Installation of the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Electrical Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Instrument Start-up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Chapter 4 AI 3000/AS 3000 II Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
Control from PC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Washes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Info Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Status Line. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
x AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Contents
Control Through the TRACE GC Ultra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Configuring the AI 3000/AS 3000 II. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
AI 3000/AS 3000 II Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Extended Control Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
When No Vial Abort Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Control Through the FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Configuring the AI 3000/AS 3000 II. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
AI 3000/AS 3000 II Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Sequence of Samples with the AS 3000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Guidelines for Programming with Different Injectors . . . . . . . . . . . . . . . . . . . . 70
SSL Inlet (TRACE GC Ultra/FOCUS GC) . . . . . . . . . . . . . . . . . . . . . . . . . 70
Nano-Volumes Injections in S/SL Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Packing a SSL Liner. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
S/SL Inlet with a Merlin Microseal™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
PTV Inlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
PTV Inlet with a Merlin Microseal™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
PKD and PPKD Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
Current Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Emptying of the Waste Container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Cleaning of the Sample Tray Accessory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Cleaning the Instrument Externally. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Replacing the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Cleaning the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Automated Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Manual Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Preparing a New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Moving Away the Sampling Unit from the Injector Module . . . . . . . . . . . . . . . 80
Abbreviations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xi
Contents
xii AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
P
Preface
This manual contains descriptions of the features and components of the AI 3000/AS 3000 II
sampling systems. Inside, you will find all of the information necessary for routine operation
of your sampling system. This includes operating procedures, sample injection techniques,
and diagrams and descriptions of the major components.
This manual is organized as follows:
• Chapter 1, “Introduction,” provides a basic overview of the features and options of the
ailable instrument configurations.
AI 3000/AS 3000 II System. It also describes the
• Chapter 2, “Sampling Unit,” provides a description of the components of the sampling
unit of the AI 3000/AS 3000 II System.
• Chapter 3, “Installation,” contains the instructions for the installation of the
AI 3000/AS 3000 II on the TRACE 1300/TRACE 1310, TRACE GC Ultra and
US GC gas chromatographs, the syringe and the electrical connections with the
FOC
different units of the gas chromatographic system.
• Chapter 4, “AI 3000/AS 3000 II Control,” provides the information to control the
AI 3000/AS 3000 II from the computer or the GC. The chapter contains also the
rk
ing procedures with different injectors.
wo
av
• Chapter 5, “Maintenance,” provides guidelines for the maintenance of the AI 3000/AS
3000 II.
• Abbreviations contains definitions of terms used in this manual. This also includes
abbreviations, acronyms, metric prefixes, and symbols.
• Index contains an alphabetical list of key terms and topics in this guide, including cross
references and the corresponding page numbers.
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xiii
Preface
About Your System
About Your System
WARNING Thermo Fisher Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment may be impaired. If you
maintain a system outside the specifications listed in this guide, failures of many types,
including personal injury or death, may occur. The repair of instrument failures caused by
operation in a manner not specified by the manufacturer is specifically excluded from the
Standard Warranty and service contract coverage.
WARNING When, for technical reasons, it is necessary to work on instrument parts
which may involve an hazard (moving parts, components under voltage, etc.) the
authorized Technical Service must be contacted. This type of situations can be identified
because access to these parts is possible only by using a tool. The removable protective
covers bear a warning symbol suggesting to refer to the documentation accompanying the
instrument. Should an operator perform a maintenance operation, he/she must have
received proper training to carry out that specific action.
WARNING Before using dangerous substances (toxic, harmful, etc.), please read the
hazard indications and information reported in the Safety Sheet supplied by the
manufacturer referring to the relevant CAS (Chemical Abstract Service) number.
The AI 3000/AS 3000 II sampling system requires the use of several chemical products,
which are present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to the
relevant CAS (Chemical Abstract Service) number.
Environmental Conditions
•Internal use
• Up to 2000 meters altitude
• Temperature 18 to 30 °C
• Maximum relative humidity between 30% and 85%
• Voltage variations must not exceed the nominal voltage by ± 10%
• Transient overloads in compliance wi
th installation categories II
• Pollution degree according to IEC 664 (3.7.3) 2
• Protection degree IP00
xiv AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Rating
Contacting Us
Preface
Rating
AI 3000/AS 3000 II Automatic Sampling System
• 24 Vdc through a portable external power supply
− input 90-264 Vac; 47/63 Hz — output 24 Vdc; 70 W
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.
Use http://www.thermoscientific.com address for products information.address for products
information.
Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to
contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer Support.
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this guide. Safety and other
special notices appear in boxes and include the following:
WARNING This is the general warning safety symbol and safety alert word to prevent
actions that could cause personal injury. It highlights hazards to humans or the
environment. Each WARNING safety alert is preceded with this safety symbol and another
appropriate safety symbol (see “Safety Alerts and Important Information” on page xv)
Then it is followed with an appropriate safety precautionary message. When you see a
safety alert on your instrument or in the publications, please carefully follow the safety
instructions before proceeding.
CAUTION This is the safety alert word to prevent actions that may cause personal injury or
instrument damage. We use it to highlight information necessary to prevent personal
injury or damage to software, loss of data, or invalid test results; or might contain
information that is critical for optimal system performance. A CAUTION safety alert is
always preceded with an appropriate safety symbol (see “Safety Alerts and Important
Information” on page xv) Then it is followed with an appropriate safety precautionary
message. When you see a safety alert on your instrument or in the publications, please
carefully follow the safety instructions before proceeding.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xv
Preface
Safety Alerts and Important Information
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury and/or instrument damage. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION in intended to prevent improper actions
that may cause personal injury and/or instrument damage. The following safety symbols may
be found on your instrument and/or in this guide:
BIOHAZARD. This symbol indicates that a biohazard will, could, or may occur.
BOOTS REQUIRED. This symbol indicates that you must wear boots when
performing a task or else physical injury could or may occur.
BURN HAZARD. This symbol indicates a hot surface could, or may incur burn
injuries.
CLOTHING REQUIRED. This symbol indicates that you should wear a work
clothing when performing a task or else physical injury could or may occur.
ELECTRICAL SHOCK HAZARD. This symbol indicates that an electrical shock
hazard could, or may occur.
EXPLOSION HAZARD. This symbol indicates an explosion hazard. This
symbol indicates this risk could or may cause physical injury.
FIRE HAZARD. This symbol indicates a risk of fire or flammability, or that
fire/flammability damage will, could, or may occur.
FLAMMABLE GAS HAZARD. This symbol alerts you to gases that are
compressed, liquefied or dissolved under pressure and can ignite on contact
with an ignition source. This symbol indicates this risk could or may cause
physical injury.
GLOVES REQUIRED. This symbol indicates that you must wear gloves when
performing a task or else physical injury could or may occur.
HAND AND CHEMICAL HAZARD. This symbol indicates that chemical
damage or physical injury will, could, or may occur.
HARMFUL. This symbol indicates that the presence of harmful material will,
could, or may occur.
INSTRUMENT DAMAGE. This symbol indicates that damage to the
instrument or module may occur. This damage may not be covered under the
standard warranty.
xvi AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Preface
3
Instrument Markings and Symbols
LIFTING HAZARD. This symbol indicates two or more people are required to
lift the object to prevent a physical injury, which could or may occur.
MATERIAL AND EYE HAZARD. This symbol indicates that eye damage will,
could, or may occur. Eye protection must be worn.
RADIOACTIVE HAZARD. This symbol indicates that the presence of
radioactive material could, or may occur.
READ MANUAL. This symbol alerts you to carefully read your instrument’s
operational instructions before usage to ensure your safety and the
instrument’s operational ability. Failing to carefully read the instructions could
or may put you at risk for a physical injury.
TOXIC SUBSTANCES HAZARD. This symbol indicates that exposure to a
toxic substance will, could, or may cause personal injury or death.
This is the general warning symbol that the ISO 3864-2 standard refers to as
the general warning signal to prevent personal injury. It is a triangle with an
exclamation mark that precedes the WARNING safety alert word. In the
vocabulary of ANSI Z535 signage, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. We use this symbol and another appropriate safety symbol to alert to an
imminent or potential hazard that could cause personal injury.
Instrument Markings and Symbols
Tab le 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on the AI 3000/AS 3000 II. See the asterisk.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
* Alternating Current
Both direct and alternating current
Three-phase alternating current
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual xvii
Preface
+-
Instrument Markings and Symbols
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
* On (Supply)
* Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
* Instruction manual symbol affixed to product. Indicates that the user must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock
* Caution, hot surface
* Caution, biohazard
In-position of a bistable push control
Out-position of a bistable push control
* Jack socket
* Symbol in compliance to the Directive 2002/96/EC on Waste Electrical
and Electronic Equipment (WEEE) placed on the european market after
August, 13, 2005.
xviii AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
1
Introduction
This chapter provides a basic overview of the features and options of the AI 3000/AS 3000 II.
It also describes the available instrument configurations.
Contents
• The AI 3000/AS 3000 II Sampling System
• Configurations of the 3000 Series Automatic Sampling System
• Technical Specifications
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 1
1
Turret
Safety Door
Injection Unit
Washing and Waste Tray
8-position Sample Tray
Introduction
The AI 3000/AS 3000 II Sampling System
The AI 3000/AS 3000 II Sampling System
The 3000 Series Automatic Sampling System, available as AI 3000 or AS 3000, consists of the
following parts, as respectively shown in Figure 1 and Figure 2.
Figure 1. AI
3000 Auto-injector
2 AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Figure 2. AS 3000 Auto-sampler
Turret
Safety Door
Injection Unit
Centering Plate
Washing and Waste Tray
Base
105-position Sample Tray
1
Introduction
The AI 3000/AS 3000 II Sampling System
Sampling Unit
The sampling unit consists of the following major components:
Base
This is the structure bearing the sampling unit components.
Turret
It consists of a vertical moving structure that accommodates the injection unit and
accomplishes the necessary movements to carry out the three main operating steps: sample
drawing, injection, syringe washing. The turret is provided with a safety door allowing access
to the syringe.
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 3
1
Introduction
The AI 3000/AS 3000 II Sampling System
Injection Assembly
Housed inside the rotating turret, it consists of a moving support (sliding plate) on which the
syringe is installed.
Washing and Waste Tray
It allows to accomodate up to four 4 mL vials of solvents to wash the syringe, and a 50 mL
plastic container to collect the washing solvents after their use.
Centering Plate
It consists of a plate provided with circular guides to center the sampling unit on the injector
nut.
Sample Tray
It contains the seats for the sample vials. Each position is numbered to make the sample
identification easy. The following options are available:
• AI 3000 Auto-injector — sample tr
• AS 3000 Auto-sampler
mobile tray mounted on a dedicated support plate.
— ample holding assembly consisting of a 105-position turnable
ay consisting of an 8-position fixed support.
Sampler Support
It constitutes the supporting base allowing to install the autoinjector/sampler on the gas
chromatograph.
User Interface
The functions of the AI 3000/AS 3000 II can be controlled through:
• a data processing system for PC with dedicated software.
• the keypad of the TRACE GC Ultra.
For further information, please refer to:
• “Configurations of the 3000 Series Automatic Sampling System” on pag
• Chapter 2, “Sampling Unit.” on page 9
• Chapter 4, “AI 3000/AS 3000 II Control,” on page 55
e 5
4 AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Configurations of the 3000 Series Automatic Sampling System
8-position Sample Tray
Configurations of the 3000 Series Automatic Sampling System
The AI 3000/AS 3000 II can be installed on the TRACE 1300/TRACE 1310, TRACE GC
Ultra, or FOCUS GC gas chromatographs for sample introduction into the following
injectors:
• TRACE 1300/TRACE 1310: S/SL, PTV, PTVBKF
• TRACE GC Ultra: S/SL, PTV, PKD, PPKD
• FOCUS GC: S/SL
The autoinjector/sampler is available in the following options:
• AI 3000 Auto-injector Configuration
• AS 3000 Auto-sampler Configuration
• Gemini Configuration
1
Introduction
AI 3000 Auto-injector Configuration
In this configuration the 8-position sample tray is used. See Figure 3.
Figure 3. AI 300
0 Auto-injector Configuration
As a function of the configuration of installation, right or left, the support will be introduced
into the relevant side housing on the base of the sampling unit. On the opposite side housing,
the centering plate will be positioned.
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 5
1
105-position Sample Tray
Introduction
Configurations of the 3000 Series Automatic Sampling System
AS 3000 Auto-sampler Configuration
In this configuration the 105-position sample tray is used, mounted on the hub provided on
the dedicated support plate.
Figure 4. AS 3
The support plates of the tray and the sampler are electrically connected to each other by
means of
the proper connecting cable.
000 Auto-sampler Configuration
As a function of the configuration of installation, right or left, the support will be introduced
into the relevant housing on the base of the sampling unit.
Gemini Configuration
Consists of the installation of two AI 3000/AS 3000 II on the same GC.
Note Gemini configuration is not possible with the FOCUS GC.
• With the TRACE 1300/TRACE 1310 — the samplers are installed on the right side, one
on
the front injector module and the other on the back injector module.
• With the TRACE GC Ultra — one sampler is installed on the left side of the GC, and
her is installed on the right side.
the ot
Each autosampler operates simultaneously on its own relevant injector. In this configuration,
both autosampler are ready to inject the sample simultaneously, according to the programmed
method that is the same for both.
6 AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
Installation
Right configuration
1
Introduction
Configurations of the 3000 Series Automatic Sampling System
The AI 3000/AS 3000 II can be installed on the right or left side of the GC according to the
following indications:
The right configuration, see Figure 5, is the default configuration for all the GC units.
Note The right configuration is the only one possible on the TRACE 1300/TRACE 1310
and FOCUS GC.
Left Configuration
Figure 5. Righ
The left configuration, see Figure 6, is only possible with the TRACE GC Ultra.
The installation of the AI 3000/AS 3000 II requires a dedicated metal support on the base of
the sampler support.
Figure 6. Le
t Configuration
f
t Configuration
WARNING If an existing AI 3000 auto-injector will be upgraded to AS 3000
auto-sampler, it is very important to switch off the AI 3000 before to install the 105
sample tray and plug-in its electrical cable. This recommendation will prevent a possible
driver failure on AI 3000’s pcb.
Gemini Configuration
Two AI 3000/AS 3000 II are installed on the same TRACE 1300/TRACE 1310 or TRACE
GC Ultra by using their own dedicated supports. Refer to “Sampling System Support” on
page 23.
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 7
1
Introduction
Technical Specifications
Technical Specifications
Technical specification of the AI 3000/AS 3000 II are listed in Tabl e 1.
Table 1. Technical Specifications
Sample tray capacity • 8 vials in the AI 3000 configuration
Vials capacity 0.3 and 2 mL (standard) with both 8- and 105-position
Flushing solvents 4 vials of 4 mL
Waste container capacity 50 mL
Types of syringes • 10 μL syringe (standard)10 μL syringe for Merlin
• 105 vials in the AS 3000 configuration
trays
Microseal Valve (optional)
•5 μL syringe (optional)
•0.5 μL syringe for Nano-volumes injections (optional)
Volumes programming (μl) • 0 to 5 μL (10 μL syringe) with increments of 0.1 μL
• 0 to 2.5 μL (5 μL syringe) with increments of 0.1 μL
• 0 to 0.5 μL (0.5 μL syringe) with increments of 0.01 μL
Programmability Remote
External interface Serial line RS 232
Power supply rating 24 Vdc through a portable external power supply
Electrical characteristics of the supply
• input 90-264 Vac; 47/63 Hz
• output 24 Vdc; 70 W
Dimensions
(height x width x length)
Mass About 6 kg
Sampling unit:
• AI Configuration: approx. 437 x 230 x 250 mm
• AS Configuration: approx. 437 x 400 x 250 mm
8 AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
2
Sampling Unit
This chapter provides a description of the components of the sampling unit of the
AI 3000/AS 3000 II.
Contents
• Base
• Turret
• Injection Assembly
• Washin g an d Wast e Tray
• Rear of the Sampling Unit
• Sample Trays
• Centering Plate
• Status LED
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 9
2
Front Section
Right Side Section
Left Side Section
Rear Section
Sampling Unit
Base
Base
It is the supporting structure of the instrument on which the sampling unit components and
accessories are positioned (sample tray, centering plate). It consists of four sections. See
Figure 7.
e
Figure 7. Th
Sections of the Base
• Front Section — It includes the housings for solvents vials and waste container.
On the lower front part there is a LED indicating the instrument status. For details, refer
to “Status LED” on pag
• Right and Left Side Sections —
e 20.
It can accommodate the, 8-position sample tray or
105-position sample tray and the centering plate for the injector.
The position of the above described accessories depends on the configuration of the
njector/auto-sampler installation.
auto-i
See “Configurations of the 3000 Series Automatic Sampling System” on
10 AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
page 5.
Turret
2
Sampling Unit
Rear Section — It includes the connections of the instrument to the mains, the gas
chromatograph, the data system and the sample tray (only for AS 3000), and for the Gemini
configuration.
The turret consists of a moving structure pivoted on a hub. The rotating movements are
accomplished by a motor located on the base. An optical sensor determines the turret travel
ends.
The front side is provided with a safety door allowing access to the injection assembly. The
door opening immediately cuts off the power supply to the sampling system.
he T
Figure 8. T
urret
Turret
Thermo Scientific AI 3000/AS 3000 Sampling System - Operating Manual 11
2
5
Safety Door
Injection Device
Sliding Plate
Syringe Plunger Control
Syringe
Vial Capture Device
Sampling Unit
Injection Assembly
Injection Assembly
It consists of a sliding plate which supports and guides the vertical movements of the
injection device constituted by the syringe plunger control, the vial capture device and the
syringe. See Figure 9.
Figure 9. In
je
ction Assembly
The vertical movements of the injection device are controlled by motors housed in the turret.
The travel ends of the movable parts is defined by a series of sensors.
Inside the turret two electronic boards are also present, one for the motors control and one for
the instrument functions control, respectively.
12 AI 3000/AS 3000 Sampling System - Operating Manual Thermo Scientific
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