Thermo Scientific
AI 1310 / AS 1310
Autosamplers
User Guide
31716000 Third Edition December 2015
© 2015 Thermo Fisher Scientific Inc. All rights reserved.
AI 1310, AS 1310, TRACE 1300, TRACE 1310, TRACE GC Ultra, FOCUS GC, Xcalibur, Chromeleon,
Chrom-Card and ChromQuest are trademarks of Thermo Fisher Scientific. EzChrom is a trademark of Agilent
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Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
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document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
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Sale shall govern all conflicting information between the two documents.
Release history: First edition, released March 2012 “Original Instructions”
Second Edition, released August 2013; Third Edition December 2015
For Research Use Only. Not for use in diagnostic procedures.
AI 1310 / AS 1310 User Guide, PN 31716000, Third Edition
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Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
•installation
•re-calibration
•changes and repairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
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Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety
standards.
Safety
This device complies with EN 61010-1:2001, EN 61010-2:2002 according to Low Voltage Directive 2006/95/EC.
Electromagnetic Compatibility
This device complies with EN 61326-1:2006 according to directive 2004/108/EC.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
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This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
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Member State, and these companies should dispose of or recycle this product. See www.thermoscientific.com/rohsweee
further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country.
for
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P
Preface
This manual contains descriptions of the features and components of the AI 1310/AS 1310
sampling systems. Inside, you will find all of the information necessary for routine operation
of your sampling system. This includes operating procedures, sample injection techniques,
and diagrams and descriptions of the major components.
This manual is organized as follows:
• Chapter 1, “Introduction,” provides a basic overview of the features and options of the AI
1310/AS 1310 System. It also describes the available instrument configurations.
• Chapter 2, “Sampling Unit,” provides a description of the components of the sampling
unit of the AI 1310/AS 1310 System.
• Chapter 3, “Installation,” contains the instructions for the installation of the
AI 1310/AS 1310 on the TRACE 1300, TRACE 1310, TRACE GC Ultra and FOCUS
GC gas chromatographs, the syringe and the electrical connections with the different
units of the gas chromatographic system.
• Chapter 4, “AI 1310/AS 1310 Control,” provides the information for controlling the
AI 1310/AS 1310 through the Chromatography Data System, the TRACE GC Ultra, or
the FOCUS GC. The chapter contains also the working procedures with different
injectors.
• Chapter 5, “Maintenance,” provides guidelines for the maintenance of the AI 1310/AS
1310.
• Glossary contains definitions of terms used in this manual. This also includes
abbreviations, acronyms, metric prefixes, and symbols.
• Index contains an alphabetical list of key terms and topics in this guide, including cross
references and the corresponding page numbers.
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide ix
Preface
About Your System
About Your System
WARNING Thermo Fisher Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment may be impaired. If you
maintain a system outside the specifications listed in this guide, failures of many types,
including personal injury or death, may occur. The repair of instrument failures caused by
operation in a manner not specified by the manufacturer is specifically excluded from the
Standard Warranty and service contract coverage.
WARNING When, for technical reasons, it is necessary to work on instrument parts
which may involve an hazard (moving parts, components under voltage, etc.) the
authorized Technical Service must be contacted. This type of situations can be identified
because access to these parts is possible only by using a tool. The removable protective
covers bear a warning symbol suggesting to refer to the documentation accompanying the
instrument. Should an operator perform a maintenance operation, he/she must have
received proper training to carry out that specific action.
WARNING Before using dangerous substances (toxic, harmful, and so forth), read the
hazard indications and information reported in the Safety Sheet supplied by the
manufacturer referring to the relevant CAS (Chemical Abstract Service) number.
The AI 1310/AS 1310 sampling system requires the use of several chemical products,
which are present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to the
relevant CAS (Chemical Abstract Service) number.
Environmental Conditions
•Internal use
• Up to 2000 meters altitude
• Temperature 18 to 30 °C
• Maximum relative humidity between 30% and 85%
• Voltage variations must not exceed the nominal voltage by ± 10%
• Transient overloads in compliance with installation categories II
• Pollution degree according to IEC 664 (3.7.3) 2
• Protection degree IP00
x AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Rating
Contacting Us
Preface
Rating
AI 1310/AS 1310 Autosampler
• 24 Vdc through a portable external power supply, level VI efficiency
− input 90-264 Vac; 47-63 Hz — output 24 Vdc; 70 W minimum
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.
Use http://www.thermoscientific.com address for products information.address for products
information.
Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to
contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer Support.
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this guide. Safety and other
special notices appear in boxes and include the following:
WARNING This is the general warning safety symbol and safety alert word to prevent
actions that could cause personal injury. It highlights hazards to humans or the
environment. Each WARNING safety alert is preceded with this safety symbol and another
appropriate safety symbol (see “Safety Alerts and Important Information” on page xi)
Then it is followed with an appropriate safety precautionary message. When you see a
safety alert on your instrument or in the publications, please carefully follow the safety
instructions before proceeding.
CAUTION This is the safety alert word to prevent actions that may cause personal injury or
instrument damage. We use it to highlight information necessary to prevent personal
injury or damage to software, loss of data, or invalid test results; or might contain
information that is critical for optimal system performance. A CAUTION safety alert is
always preceded with an appropriate safety symbol (see “Safety Alerts and Important
Information” on page xi) Then it is followed with an appropriate safety precautionary
message. When you see a safety alert on your instrument or in the publications, please
carefully follow the safety instructions before proceeding.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xi
Preface
Safety Alerts and Important Information
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury and/or instrument damage. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION in intended to prevent improper actions
that may cause personal injury and/or instrument damage. The following safety symbols may
be found on your instrument and/or in this guide:
Symbol Descriptor
BIOHAZARD: Indicates that a biohazard will, could, or might occur.
BURN HAZARD: Alerts you to the presence of a hot surface that could or might
cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.
FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.
EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates
this risk could or might cause physical injury.
FLAMMABLE GAS HAZARD. Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
CLOTHING REQUIRED. Indicates that you should wear a work clothing when
performing a task or else physical injury could or might occur.
BOOTS REQUIRED. Indicates that you must wear boots when performing a
task or else physical injury could or might occur.
MATERIAL AND EYE HAZARD. Indicates you must wear eye protection when
performing a task.
HAND AND CHEMICAL HAZARD: Indicates that chemical damage or physical
injury could or might occur.
xii AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Preface
3
Instrument Markings and Symbols
HARMFUL. Indicates that the presence of harmful material will, could, or
might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.
LIFTING HAZARD. Indicates that a physical injury could or might occur if two
or more people do not lift an object.
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for a
physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.
LASER HAZARD. Indicates that exposure to a laser beam will, could, or might
cause personal injury.
RADIOACTIVE HAZARD. Indicates that the presence of radioactive material
could or might occur.
For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard. In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.
Instrument Markings and Symbols
Ta b le 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on the AI 1310/AS 1310. See the asterisk.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
* Alternating Current
Both direct and alternating current
Three-phase alternating current
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xiii
Preface
+-
Safety Information and Warnings
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
* On (Supply)
* Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
* Instruction manual symbol affixed to product. Indicates that the user must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock
* Caution, hot surface
* Caution, biohazard
In-position of a bistable push control
Out-position of a bistable push control
* Jack socket
* Symbol in compliance to the Directive 2012/19/EU on Waste Electrical
and Electronic Equipment (WEEE) placed on the European market after
August, 13, 2005.
Safety Information and Warnings
This safety guide raises awareness of potential safety issues and general points for
consideration for Thermo Fisher Scientific representatives during installation, and repair of
the AI 1310 /AS 1310 sampling system., or parts of it (following the life cycle principle), as
well as for the end user AI 1310 /AS 1310 sampling system in the lab during the learning
phase, and in routine work.
xiv AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
IMPORTANT Read this section first before operating the AI 1310 /AS 1310 Autosamplers.
General Considerations
• Before a unit is put to use, consult the current User Guide and related documents under
all circumstances.
• Changes or modifications to this unit not expressly approved by the party responsible for
compliance, could void your’s authority to operate the equipment.
• Be aware that if the equipment is used in a manner not specified by the manufacturer, the
protective and safety features of the equipment might be impaired.
• The repair of instrument failures caused by operation in a manner not specified by the
manufacturer is expressly excluded from the standard warranty and service contract
coverage.
Preface
Safety Information and Warnings
Electrical Hazards
• When, for technical reasons, it is necessary to work on instrument parts which may
involve an hazard (moving parts, components under voltage, etc.) the authorized
Technical Service must be contacted. This type of situations can be identified because
access to these parts is possible only by using a tool. The removable protective covers bear
a warning symbol suggesting to refer to the documentation accompanying the
instrument. Should an operator perform a maintenance operation, he/she must have
received proper training to carry out that specific action.
• Before using dangerous substances (toxic, harmful, and so forth), read the hazard
indications and information reported in the Safety Sheet supplied by the manufacturer
referring to the relevant CAS (Chemical Abstract Service) number.
The AI 1310/AS 1310 sampling system requires the use of several chemical products,
which are present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to the
relevant CAS (Chemical Abstract Service) number.
Every analytical instrument has specific hazards. Be sure to read and comply with the
following pre-cautions. They ensure the safe and long-term use of your AI 1310 /AS 1310
Autosamplers.
The installation over-voltage category is Level II. The Level II category pertains to equipment
receiving its electrical power from the local level, such as an electrical wall outlet.
Connect the AI 1310/AS 1310 sampling system only to instruments complying with IEC
61010 safety regulations.
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xv
Preface
Safety Information and Warnings
The power line and the connections between the AI 1310/AS 1310 sampling system and
other instruments, used in the configuration setup of the total analytical system, must
maintain good electrical grounding. Poor grounding represents a danger for the operator, and
might seriously affect the performance of the instrument.
Do not connect the AI 1310/AS 1310 sampling system to power lines that supply devices of a
heavy duty nature, such as motors, refrigerators and other devices that can generate electrical
disturbances.
Pay attention not to leave any cable connecting the AI 1310/AS 1310 sampling system and
the chromatographic system, or the power cord close to heated zone, such as the injector or
detector heating blocks, or the GC hot air vents.
Always replace any cable showing signs of damage with another one provided by the
manufacturer. Safety regulations must be respected.
Do not change the external or internal grounding connections. Tampering with or
disconnecting these connections could endanger you and damage the AI 1310/AS 1310
sampling system.
The instrument is properly grounded in accordance with these regulations when shipped.
To ensure safe operation, you do not must make any changes to the electrical connections or
the instrument's chassis.
Do not turn the instrument on if you suspect that it has incurred any type of electrical
damage. Instead, disconnect the power cord and contact a Thermo Fisher Scientific
representative for a product evaluation. Do not attempt to use the instrument until it has been
evaluated. Electrical damage might have occurred if the AI 1310/AS 1310 sampling system
shows visible signs of damage, exposure to any liquids or has been transported under severe
stress.
Damage can also result if the instrument is stored for prolonged periods under unfavorable
conditions: for example, subjected to heat, moisture, and so on. Ensure that the power
supply/controller unit is always placed in a clean and dry position. Avoid any liquid spills in
the vicinity.
Before attempting any type of maintenance work, always disconnect the power cords from the
power supply if optional devices are installed. Capacitors inside the instrument might still be
charged also if the instrument is turned off.
To avoid damaging electrical parts, do not disconnect an electrical assembly while power is
applied to the AI 1310/AS 1310 sampling system. After the power is turned off, wait
approximately 30 seconds before you disconnect an assembly.
The instrument includes a number of integrated circuits. These circuits might be damaged if
exposed to excessive line voltage fluctuations, power surges or electrostatic charges, or both.
Never try to repair or replace any components of the instrument without the assistance of a
Thermo Fisher Scientific representative. There are no operator-serviceable or replaceable parts
inside the power supply or in the AI 1310/AS 1310 sampling system. If a power supply is not
functioning, contact a Thermo Fisher Scientific representative.
xvi AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Other Hazards
Preface
Safety Information and Warnings
To avoid injury and possible infection through contamination during AI 1310/AS 1310
sampling system operation, keep your hands away from the syringe.
Danger of crushing to fingers and hands. To avoid injury keep your hands away from moving
parts during operation. Turn off the power to the AI 1310/AS 1310 sampling system if you
must reach inside a mechanically powered system with moving parts.
To avoid injury, observe safe laboratory practice when handling solvents, or operating the AI
1310/AS 1310 sampling system. Know the physical and chemical properties of the solvents
you use. See the MSDS (Material Safety Data Sheets) from the manufacturer of the solvents
being used.
When using the AI 1310/AS 1310 sampling system, follow the generally accepted procedures
for quality control and method development.
Do not operate on the instrument components that form part of the work area of the
AI 1310/AS 1310 sampling system when it is in motion.
Do not use vials without a sealing cap without a plate seal. Vapor phase from organic solvents
can be hazardous and flammable. Acidic vapor phase can cause corrosion to critical
mechanical parts.
Do not reuse the vials. During the process of washing the vial, micro-cracks can form which
will weaken the glass wall and increase the chances of the vial breaking.
Working with Toxic or other Harmful Compounds
WARNING Before using hazardous substances (toxic, harmful, and so on), please read the
hazard indications and information reported in the applicable Material Safety Data Sheet
(MSDS). Use personal protective equipment according to the safety requirements.
Before using dangerous substances (toxic, harmful, and so on) read the hazard indications and
information reported in the Material Safety Data Sheet (MSDS) supplied by the
manufacturer, referring to the relevant CAS (Chemical Abstract Service) number. AI 1310/AS
1310 sampling system requires the use of several chemical products with different hazard
characteristics, which are present in vials and syringes. Before using these substances or
replacing the syringe, please read the hazard indications and information reported in the
MSDS supplied by the manufacturer referring to the relevant CAS number.
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xvii
Preface
Safety Information and Warnings
Maintenance
When preparing the samples, please refer to local regulations for the ventilation conditions of
the work room.
All waste materials must be collected and eliminated in compliance with the local regulations
and directives in the country where the instrument is used.
Any external cleaning or maintenance must be performed with the AI 1310/AS 1310
sampling system turned off and the power cord disconnected. Avoid using solvents and
spraying on electrical parts. For the removal of potentially dangerous substances (toxic,
harmful, and so on) read the hazard indications and information reported in the MSDS
(Material Safety Data Sheet) supplied by the manufacturer referring to the relevant CAS
(Chemical Abstract Service) number. Use proper protective gloves.
When working with hazardous materials such as radioactive, biologically hazardous material,
and so on, it is important to train all operators how to respond in case of spills or
contamination.
Disposal
Depending on the class of hazardous material, the appropriate measures have to be taken
immediately. Therefore, the chemicals or solvents needed for decontamination have to be on
hand.
Any parts of the equipment which can potentially be contaminated, such as the sample vial
rack, syringe tool, wash module, and so on, must be cleaned regularly. The waste solvent from
cleaning and any hardware which requires to be disposed of has to be properly eliminated
with all the necessary precautions, abiding by national and international regulations.
When preparing for decontamination, ensure that the solvent or chemical to be used will not
damage or react with the surface, dye (color) of the instrument, table or other nearby objects.
If in doubt, please contact your Thermo Fisher Scientific representative to verify the
compatibility of the type or composition of solvents with the AI 1310/AS 1310 sampling
system.
It is your responsibility to handle hazardous chemicals or biological compounds, including
(but not limited to) bacterial or viral samples and the associated wastes, safely and in
accordance with international and local regulations.
Do not dispose of this equipment or parts thereof unsorted in municipal waste.
Follow local municipal waste regulations for proper disposal provisions to reduce
the environmental impact of waste electrical and electronic equipment (WEEE).
European Union customers: Call your local customer service representative responsible for the
AI 1310/AS 1310 sampling system for complimentary equipment pick-up and recycling.
xviii AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
WARNING The customer has to ensure that the AI 1310/AS 1310 sampling system has
not been contaminated by any hazardous chemical or biological compounds including
(but not limited to) bacteria or viruses.
Any part which had direct contact with the analytical sample must be identified and must
undergo an appropriate decontamination procedure prior to shipping for disposal.
Potentially dangerous components are: syringes and vials. Any critical parts sent for
disposal must be handled according to national laws for hazardous compounds.
The customer and the service engineer are fully responsible for enforcing these
requirements. Thermo Fisher Scientific will hold the representative, customer responsible,
or both, if these regulations are not observed.
Hazardous Substances Precautions
Preface
Hazardous Substances Precautions
WARNING Before using hazardous substances (toxic, harmful, and so on), read the
hazard indications and information reported in the applicable Material Safety Data Sheet
(MSDS.) Use Personal protection according to the safety requirements.
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xix
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Rating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Safety Information and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
General Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Electrical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Other Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Working with Toxic or other Harmful Compounds . . . . . . . . . . . . . . . . . . xvii
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xviii
Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Hazardous Substances Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xix
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
The AI 1310/AS 1310 Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Configurations of the 1310 Series Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . 5
AI 1310 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
AS 1310 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Gemini Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 2 Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Turret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Injection Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Vial Capture Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Turret Movements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Washing and Waste Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xxi
Contents
Back of the Sampling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Sample Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
8-Position Sample Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
105-Position Sample Tray Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
155-Position Sample Tray Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Centering Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Centering Plate for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . 20
Centering Plate for TRACE GC Ultra and FOCUS GC. . . . . . . . . . . . . . . . 22
Status LED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Who Performs the Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
How to Lift and Carry the Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Sampling System Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Sampling System Support for TRACE 1300/TRACE 1310 . . . . . . . . . . . . . 27
Sampling System Support for TRACE GC Ultra. . . . . . . . . . . . . . . . . . . . . . 30
Sampling System Support for FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . 32
References for the Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Installing the Sampling System Support on the TRACE 1300/1310 GC . . . . . 33
Assembling of the Sampling System Support on the Front Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Assembling of the Sampling System Support on the Back Injector
Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Assembling of the Sampling System Support for the Gemini
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Assembling of the Sampling System Support When a TSQ Quantum
is Coupled with the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Installing the Sampling System Support on the TRACE GC Ultra . . . . . . . . . . 42
Installing the Spacer Inside the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Assembling of the Sampling System Support on the GC Right Side . . . . . . . 43
Assembling of the Sampling System Support on the GC Left Side. . . . . . . . . 44
Installing the Sampling System Support on the FOCUS GC. . . . . . . . . . . . . . . 44
Assembling of the Sampling System Support on the GC right side . . . . . . . . 45
Assembling of the Sampling System Support on FOCUS GC MS
Version. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Space Saving Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Installation of the AI 1310/AS 1310 on the GC . . . . . . . . . . . . . . . . . . . . . . . . 48
Installation of the Sampling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Installation of the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Electrical Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Instrument Start-up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
xxii AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Contents
Chapter 4 AI 1310/AS 1310 Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Control Through the Chromatography Data System. . . . . . . . . . . . . . . . . . . . . 58
User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Chromeleon CDS User Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Xcalibur, Chrom-Card, and ChomQuest CDS User Interface. . . . . . . . . . . . 61
Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Control Through the TRACE GC Ultra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Configuring the AI 1310/AS 1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
AI 1310/AS 1310 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Extended Control Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
When No Vial Abort Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Control Through the FOCUS GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Configuring the AI 1310/AS 1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
AI 1310/AS 1310 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Sequence of Samples with the AS 1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Guidelines for Programming with Different Injectors . . . . . . . . . . . . . . . . . . . . 69
SSL Inlet (TRACE 1300/1310 GC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
SSL Inlet (TRACE GC Ultra/FOCUS GC) . . . . . . . . . . . . . . . . . . . . . . . . . 71
SSL Inlet with a Merlin Microseal™ (TRACE GC Ultra Only) . . . . . . . . . . . 74
PTV Inlet (TRACE 1300/1310 GCTRACE GC Ultra) . . . . . . . . . . . . . . . . 75
PTV Inlet with a Merlin Microseal™ (TRACE 1300/1310 GC,
TRACE GC Ultra). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
PKD and PPKD Inlets (TRACE GC Ultra, FOCUS GC) . . . . . . . . . . . . . . 76
Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Current Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Emptying of the Waste Container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Cleaning of the Sample Tray Accessory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Cleaning the Instrument Externally. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Replacing the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Cleaning the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Automated Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Manual Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Preparing a New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Moving Away the Sampling Unit from the Injector Module . . . . . . . . . . . . . . . 82
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide xxiii
1
Introduction
This chapter provides a basic overview of the features and options of the AI 1310/AS 1310.
The available instrument configurations are also described.
Contents
• The AI 1310/AS 1310 Sampling System
• Configurations of the 1310 Series Autosampler
• Technical Specifications
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide 1
1
Turret
Base
Safety Door
Injection Unit
Centering Plate
Washing and Waste Tray
8-position Sample Tray
Introduction
The AI 1310/AS 1310 Sampling System
The AI 1310/AS 1310 Sampling System
The 1300 Series Autosampler, available as AI 1310 or AS 1310, consists of the following
parts, as respectively shown in Figure 1 and Figure 2.
Figure 1. AI 1310 Sampling System
2 AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Turret
Safety Door
Injection Unit
Centering Plate
Washing and Waste Tray
Base
105-position Sample Tray or
155-position Sample Tray
AS 1310 with 105-position Sample Tray
AS 1310 with 155-position Sample Tray
Figure 2. AS 1310 Sampling System
1
Introduction
The AI 1310/AS 1310 Sampling System
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide 3
1
Introduction
The AI 1310/AS 1310 Sampling System
Sampling Unit
The sampling unit consists of the following major components:
Base — This is the structure bearing the sampling unit components.
Tu r r e t — Consists of a vertical moving structure that accommodates the injection unit and
accomplishes the necessary movements to carry out the three main operating steps: sample
drawing, injection, syringe washing. The turret is provided with a safety door allowing access
to the syringe.
Injection Assembly — Housed inside the rotating turret, it consists of a moving support
(sliding plate) on which the syringe is installed.
Washing and Waste Tray — Accommodate up to four 4 mL vials of solvents to wash the
syringe, and a 50 mL plastic container to collect the washing solvents after their use.
Centering Plate — Consists of a plate provided with circular guides to center the sampling
unit on the injector nut.
Sample Tray — Contains the seats for the sample vials. Each position is numbered to make
the sample identification easy. The following options are available:
• Sample tray for AI 1310 consisting of an 8-position fixed support.
• Sample holding assembly for AS 1310 consisting of a 105-position turnable mobile
tray mounted on a dedicated support plate.
• Sample holding assembly for AS 1310 consisting of a 155-position turnable mobile
tray mounted on a dedicated support plate.
Sampler Support — Constitutes the supporting base allowing to install the sampler on the
gas chromatograph.
User Interface — The functions of the AI 1310/AS 1310 can be controlled through:
• a data processing system for PC with dedicated software.
• the keypad of the TRACE GC Ultra.
For further information, please refer to:
• “Configurations of the 1310 Series Autosampler” on page 5
• Chapter 2, “Sampling Unit,” on page 11
• Chapter 4, “AI 1310/AS 1310 Control,” on page 57
4 AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
Configurations of the 1310 Series Autosampler
8-position Sample Tray
The AI 1310/AS 1310 can be installed on the TRACE 1300/TRACE 1310, TRACE GC
Ultra, or FOCUS GC gas chromatographs for sample introduction into the following
injectors:
• TRACE 1300/TRACE 1310: SSL, SSLBKF, PTV, and PTVBKF injectors
• TRACE GC Ultra: SSL, PTV, PKD, and PPKD injectors
• FOCUS GC: SSL injector
The sampler is available in the following options:
• AI 1310 Configuration
• AS 1310 Configuration
• Gemini Configuration
1
Introduction
Configurations of the 1310 Series Autosampler
AI 1310 Configuration
In this configuration the 8-position sample tray is used. See Figure 3.
Figure 3. AI 1310 Configuration
As a function of the right or left installation configuration, the support will be introduced into
the relevant side housing on the base of the sampling unit. On the opposite side housing, the
proper centering plate will be positioned.
Thermo Scientific AI 1310/AS 1310 Sampling System - User Guide 5
1
105-position Sample Tray
155-position Sample Tray
Introduction
Configurations of the 1310 Series Autosampler
AS 1310 Configuration
In this configuration the 105-position or 155-position sample tray is used, mounted on the
hub provided on the own dedicated support plate. See Figure 4.
Figure 4. AS 1310 Configuration
The support plates of the tray and the sampler are electrically connected to each other by the
proper connecting cable.
As a function of the right or left installation configuration, the support will be introduced into
the relevant housing on the base of the sampling unit.
Gemini Configuration
Consists of the installation of two AI 1310/AS 1310 on the same GC.
Note Gemini configuration is not possible with the FOCUS GC.
• With the TRACE 1300/TRACE 1310 — the autosamplers are installed on the right side,
• With the TRACE GC Ultra — one sampler is installed on the left side of the GC, and
Each sampler operates simultaneously on its own relevant injector. In this configuration, both
sampler are ready to inject the sample simultaneously according to the programmed analytical
method that could be the same for both or different. See “Gemini Configuration” on page 53.
one on the Front injector module, and the other on the Back injector module.
the other is installed on the right side.
6 AI 1310/AS 1310 Sampling System - User Guide Thermo Scientific
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