Page 1
Thermo Scientific Dionex
UltiMate 3000 Series
Automated Fraction Collector
AFC-3000
Operating Instructions
(Original Operating Instructions)
Revision: 1.2
Date: October 2013
© 2013 Thermo Fisher Scientific
Doc No. 4820.7250
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Declaration of Conformity
(Original Declaration)
Product: Thermo Scientific Dionex UltiMate 3000 - Fraction Collector
Type: AFC-3000
Dionex Softron GmbH herewith declares conformity of the above products with
the respective requirements of the following regulations:
• EMC Directive 2004/108/EC
• Low Voltage Directive 2006/95/EC
The electrical safety of the products was evaluated based on the following
standard:
• DIN EN 61010-1:2001
Safety requirements for electrical equipment for measurement, control and
laboratory use, Part 1: General Requirements
The Electromagnetic Compatibility (EMC) of the products was evaluated based
on the following standard:
• DIN EN 61326: 2006
Electrical equipment for measurement, control and laboratory use
EMC Requirements
This declaration is issued for the manufacturer
Dionex Softron GmbH
Part of Thermo Fisher Scientific Inc.
Dornierstraße 4
D-82110 Germering
by the Managing Director, Rüdiger Obst and
the Vice President HPLC, Fraser McLeod.
August 30th, 2013
Operating Instructions Page I
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Page II Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Table of Contents
1 Introduction ................................................................................................................... 1
1.1 How to Use This Manual ........................................................................................... 1
1.2 Safety Information ..................................................................................................... 2
1.2.1 Symbols on the Fraction Collector and in the Manual ...................................... 2
1.2.2 Safety Precautions .............................................................................................. 3
1.2.3 Consignes de Sécurité ........................................................................................ 6
1.3 Intended Use ............................................................................................................ 10
1.4 Federal Communications Commission (FCC) Note ................................................ 11
2 Overview ...................................................................................................................... 13
2.1 Unit Description ....................................................................................................... 13
2.2 Operating Principle .................................................................................................. 14
2.3 Fraction Collector Configurations ........................................................................... 15
2.4 Front Panel Elements ............................................................................................... 17
2.5 Rear Panel ................................................................................................................ 18
2.5.1 Power Switch ................................................................................................... 19
2.5.2 USB Port .......................................................................................................... 19
2.5.3 RS-232 (Serial) Port ......................................................................................... 19
2.5.4 Auxiliary I/O Connection ................................................................................ 19
2.6 Universal Fraction Tray, Racks and Tubes .............................................................. 20
2.7 Chromeleon Software .............................................................................................. 21
3 Installation ................................................................................................................... 23
3.1 Facility Requirements .............................................................................................. 23
3.2 Unpacking ................................................................................................................ 24
3.3 Installing the Fraction Collector .............................................................................. 26
3.3.1 Mounting the Diverter Valve Assembly .......................................................... 27
3.3.2 Diverter Valve Connections ............................................................................. 28
3.3.3 Attaching the Rinse Station ............................................................................. 29
3.3.4 Connecting the Drain Tubing .......................................................................... 29
3.3.5 Placing Fraction Racks on the Universal Drip Tray ........................................ 30
3.3.6 Adjusting the Drop Former Height .................................................................. 33
3.4 Connecting the Fraction Collector ........................................................................... 35
3.4.1 Connecting the External Desktop Power Supply ............................................. 35
3.4.2 General Communications Information ............................................................ 36
3.4.3 Communication via RS-232 ............................................................................. 36
3.4.4 Communication via USB ................................................................................. 36
3.5 Setting Up the Fraction Collector in Chromeleon ................................................... 39
3.5.1 Installing the Fraction Collector ...................................................................... 39
3.5.2 Configuring the Fraction Collector .................................................................. 40
4 Verifying Installation .................................................................................................. 43
4.1 Overview of Actions ................................................................................................ 43
4.2 Inspecting Components Prior to Fluidic Operation ................................................. 43
Operating Instructions Page i
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UltiMate 3000 Series:
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4.3 Testing the Interface ................................................................................................. 43
4.4 Testing the Drop Former Alignment ........................................................................ 44
5 Operation and Maintenance ...................................................................................... 45
5.1 Power-Up ................................................................................................................. 45
5.2 Operation with Chromeleon ..................................................................................... 45
5.2.1 Connecting to Chromeleon .............................................................................. 46
5.2.2 Direct Control .................................................................................................. 46
5.2.3 Setting the Rack Configuration ........................................................................ 49
5.2.4 Defining the Collect Mode ............................................................................... 50
5.2.5 Defining the Movement Mode ......................................................................... 51
5.2.6 Automated Fraction Collection with Chromeleon ........................................... 52
5.3 Shutting Down the Fraction Collector ..................................................................... 57
5.4 Flushing the Rinse Station and Eluent Flow Path .................................................... 57
5.5 Routine and Preventive Maintenance ...................................................................... 58
6 Troubleshooting .......................................................................................................... 59
6.1 General Notes ........................................................................................................... 59
6.2 Power and Communication Problems ...................................................................... 59
6.3 Messages in the Chromeleon Audit Trail ................................................................ 60
7 Service .......................................................................................................................... 61
7.1 General Notes and Safety Precautions ..................................................................... 61
7.2 Cleaning the Fraction Collector ............................................................................... 62
7.2.1 Daily External Cleaning ................................................................................... 62
7.2.2 Weekly Cleaning .............................................................................................. 63
7.3 Checking for Leaks .................................................................................................. 63
7.4 Installing the Kit for Normal-Phase LC ................................................................... 64
7.5 Installing the Kit for Low Flow Rates ..................................................................... 66
7.6 Replacing the Diverter Valve ................................................................................... 67
7.7 Replacing the Universal Drip Tray .......................................................................... 69
7.8 Updating the Firmware ............................................................................................ 69
8 Technical Information ................................................................................................ 71
9 Accessories, Spare Parts, and Consumables............................................................. 73
9.1 Standard Accessories (included in the shipment) .................................................... 73
9.2 Optional Accessories................................................................................................ 74
9.3 Consumables and Spare Parts .................................................................................. 75
10 Appendix - Chromeleon Example Program ............................................................. 77
11 Index ............................................................................................................................. 81
Page ii Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
1 Introduction
1.1 How to Use This Manual
The layout of this manual is designed to provide quick reference to the sections of interest
to the reader. However, in order to obtain a full understanding of the fraction collector,
Thermo Fisher Scientific recommends that you review the manual thoroughly before
operating the fraction collector.
All descriptions in the manual apply to the AFC-3000 Automated Fraction Collector.
Therefore, the term "the fraction collector" or "the AFC-3000" is used throughout the manual.
Notes: The device configuration may vary, for example, the fraction collector may be
equipped with differing rack configurations; therefore, not all descriptions
necessarily apply to your particular instrument. If some detail applies to only
one fraction collector model or version, that model (version) is identified by
name.
It may happen that the representation of a component in this manual is different
from the real component. However, this does not influence the descriptions.
The descriptions in this manual refer to firmware version 1.03 and
Chromeleon
TM
6.80 Service Release 13.
This manual is provided "as is." Every effort has been made to supply complete and
accurate information and all technical specifications have been developed with utmost
care. The information contained in this manual should not be construed as a commitment
by Thermo Fisher Scientific. Thermo Fisher Scientific assumes no responsibility for any
errors that may appear in this document that is believed to be complete and accurate at the
time of publication and, in no event shall Thermo Fisher Scientific be liable for incidental
or consequential damages in connection with or arising from the use of this document. We
appreciate your help in eliminating any errors that may appear in this document. The
information contained in this document is subject to change without notice.
All rights reserved, including those for photomechanical reproduction and storage on
electronic media. No part of this publication may be copied or distributed, transmitted,
transcribed, stored in a retrieval system, or transmitted into any human or computer language,
in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or
disclosed to third parties without the express written permission of Thermo Fisher Scientific
Inc.
Trademarks
PEEK is a trademark of Victrex PLC.
Simriz is a trademark of Carl Freudenberg KG.
Windows and Windows Vista are registered trademarks of Microsoft Corp.
All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.
Operating Instructions Page 1
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
1.2 Safety Information
The CE Mark label on the instrument indicates that the instrument is compliant with the
related standards (→ page I).
1.2.1 Symbols on the Fraction Collector and in the Manual
The table below shows the symbols used on the fraction collector:
Symbol Description
Direct current—Courant continu
Power supply is on (−) — L'instrument est mis sous tension (−) and
Power supply is off (O)— L'instrument est mis hors tension (O)
Pinch point hazard—Risque de pincement
Refer to the Operating Instructions to prevent risk of harm to the operator and to
protect the instrument against damage.
Référez-vous à ce manuel pour éviter tout risque de blessure à l'opérateur et/ou
protéger l'instrument contre tout dommage.
Label according to the "Measures for Administration of the Pollution Control of
Electronic Information Products" (China RoHS) guideline
Étiquette "Measures for Administration of the Pollution Control of Electronic
Information Products" (China RoHS)
WEEE (Waste Electrical and Electronic Equipment) label—For more
information, see the WEEE Information section in the "Installation and
Qualification Documents for Chromatography Instruments" binder.
Étiquette DEEE (Déchets d'Equipements Electriques et Electroniques) — Pour
plus d'informations, référez-vous au chapitre WEEE Information dans le
classeur "Installation and Qualification Documents for Chromatography
Instruments".
Page 2 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
At various points throughout the manual, messages of particular importance are indicated by
certain symbols:
Tip: Indicates general information, as well as information intended to
optimize the performance of the instrument.
Important: Indicates that failure to take note of the accompanying information
could cause wrong results or may result in damage to the instrument.
Important: Indique que ne pas tenir compte de l'information jointe peut conduire
à de faux résultat ou endommager l'instrument.
Warning: Indicates that failure to take note of the accompanying information
may result in personal injury.
Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner
des blessures corporelles.
1.2.2 Safety Precautions
When working with analytical instrumentation, you must know the potential hazards of
using chemical solvents.
Tip: Before initial operation of the fraction collector, make yourself
familiar with the contents of this manual.
For the safety precautions in French, see page Fehler! Textmarke
nicht definiert..
Warning: All users of the device must observe the following safety precautions
and all additional safety precautions in this manual to avoid the
possibility of personal injury or damage to the device when
operating the device or carrying out any maintenance or service
procedures.
Observe any warning labels on the device and refer to the related
sections in these Operating Instructions.
• Protective equipment
When performing any work on or near the HPLC system, wear personal protective
equipment (protective clothing, safety gloves, safety glasses) as required by the hazard of
the mobile phase and sample. For information about the proper handling of a particular
substance and for advice on specific hazards, refer to the material safety data sheet for the
substance you are using. Observe the guidelines of Good Laboratory Practice (GLP).
An eyewash facility and a sink should be close to the device. If any substance splashes on
the eyes or skin, wash the affected area and seek medical attention.
Operating Instructions Page 3
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• Hazardous substances
Many organic solvents, mobile phases and samples are harmful to health. Be sure that
you know the toxic and infectious properties of all substances that you are using. You
may not know the toxic or infectious properties of many substances that you are using. If
you have any doubt about a substance, treat it as if it contains a potentially harmful
substance. For advice on the proper handling of a particular substance, refer to the Safety
Data Sheet (SDS) of the manufacturer. Observe the guidelines of Good Laboratory
Practice (GLP).
Dispose of waste substance in an environmentally safe manner that is consistent with all
local regulations. Do not allow flammable, toxic, and/or infectious substances to
accumulate. Follow a regulated, approved waste disposal program. Never dispose of
flammable, toxic, and/or infectious substances through the municipal sewage system.
• Hazardous gases
Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample
includes volatile or flammable solvents, do not allow them to enter the workspace. If the
mobile phase or sample includes volatile or flammable solvents, avoid open flames and
sparks.
• Electrostatic discharge
Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard.
This effect is particularly pronounced in insulating capillaries and with non-conductive
solvents (for example, pure acetonitrile).
Take appropriate measures to prevent the generation of static electricity near the HPLC
system. For example, make sure that the air humidity level in the laboratory is
sufficiently high and provide proper ventilation, wear anti-static clothing or shoes,
prevent accumulation of air bubbles in waste lines, and use grounded waste containers.
Use only non-conductive capillaries to direct solvents into the waste container. With
electrically conductive capillaries, make sure that they are properly grounded.
Operation with Normal-Phase Eluents:
Do not perform normal-phase applications without a proper grounding protection for the
drop former. Non-conductive, non-polar normal-phase eluents, for example hexane, can
lead to a build-up of electrostatic charges on an ungrounded drop former. The
electrostatic discharge can create a fire and/or explosion hazard.
Note that a grounded drop former (part no 6702.0400) is available for normal-phase (NP)
applications. Use normal-phase eluents with the AFC-3000 Automated Fraction
Collector only after it has been modified with this grounded drop former. Operating the
device without the grounded drop former can result in a fire and/or explosion
hazard!
• Self-ignition of solvents
Do not use solvents for which the self-ignition temperature is below 150 °C. In case of
leakage, these solvents may self-ignite on a hot surface.
Page 4 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• Capillaries, capillary connections, open connections
♦ Capillaries, especially non-metallic capillaries may burst, slip out of their fittings or
may not be screwed in. This may result in substances spraying out of the open
connections.
♦ In an UltiMate 3000 system, some components are made of PEEK™. This polymer
has superb chemical resistance to most organic solvents. However, it tends to swell
when in contact with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or
tetrahydrofuran (THF). In addition, it is attacked by concentrated acids, such as,
sulfuric acid and nitric acid or a mixture of hexane, ethyl acetate, and methanol. In
both cases, capillaries may start leaking or they can burst. Swelling or attack by
concentrated acids is not a problem with brief flushing procedures.
♦ Do not use tubing that is stressed, bent, kinked, or damaged.
♦ Capillary connections can be contaminated by harmful substances or harmful
substances can escape from open connections.
♦ Always wear safety glasses when handling fused silica tubing, for example, during
installation or when cutting capillaries to the length.
• Disconnect the fraction collector from all power sources before removing the panels.
When the panels are removed, dangerous electrical connections will be exposed. The
enclosure must be opened only by Thermo Fisher Scientific service personnel.
• Replace faulty communication cables.
• Replace faulty power cords. Never use a power cord other than the power cords provided
for the device.
• Use only the original spare parts and accessories authorized for the device by Thermo
Fisher Scientific.
• When operating the HPLC system, always set a lower pressure limit for the pump. This
prevents damage resulting from leakage or from running the pump dry.
• The fraction collector weighs more than 8 kg (17.7 lbs). Therefore, you should use
caution when lifting or moving the fraction collector.
• When lifting or moving the autosampler, always lift by the bottom or sides of the unit.
This is to avoid damage to the instrument.
• After operation, rinse out buffers and solutions that form peroxides.
• Before switching from buffer to organic solution, rinse the analytical system thoroughly
with deionized or HPLC grade water.
• When switching to another solvent, ensure that the new solvent is miscible with the one
contained in the HPLC system. If the solvents are not miscible, the system can be
damaged, for example, by flocculation.
Operating Instructions Page 5
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• If a leak occurs, turn off the fraction collector immediately, stop the pump flow, and
remedy the situation.
• Use only standard solvents (HPLC grade) and buffers that are compatible with all parts
that may be exposed to solvents.
• To avoid personal injury, do not reach inside the sample area during a running analysis.
• Before interrupting operation for several days or more or when preparing the fraction
collector for transport, observe the precautions for shutting down the instrument
(→ page Fehler! Textmarke nicht definiert.).
• Do not use the fraction collector in ways other than those described in these Operating
Instructions.
• Keep the operating instructions near the device to be available for quick reference.
1.2.3 Consignes de Sécurité
Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation
de produit chimiques.
Veuillez noter: Avant de commencer à utiliser l'instrument, assurez-vous que vous
vous êtes familiarisés avec le contenu de ce manuel.
Avertissement: Toutes les personnes utilisant l’instrument doivent observer les
consignes de sécurité suivantes et dans les autres chapitres de ce
manuel pour éviter une mise en danger de sa personne ou de
dommage à l’instrument pendant l’utilisation et des opérations de
maintenance ou service de l’instrument.
Observez les étiquettes d'avertissement sur l'instrument et référez-
vous aux sections correspondantes dans ce mode d'emploi.
• Equipment de protection
Pour tous les travaux sur le système HPLC ou à proximité, portez l'équipement de
protection personnel (vêtements de protection, gant de sécurité, lunettes de protection)
qui correspond aux risque découlant de la phase mobile et/ou de l'échantillon. Pour les
informations sur la manipulation correcte des composés et des recommandations pour les
situations de risque spécifiques, veuillez consulter la fiche de données de sécurité des
substances que vous utilisez. Veuillez respecter des directives des Bonnes Pratiques de
Laboratoire (BPL).
Une installation permettant de se laver les yeux ainsi qu'un lavabo doivent se trouver à
proximité du système. Si une substance, quelle qu'elle soit, entre en contact avec vos
yeux ou votre peau, rincez abondamment la zone affectée à l’eau, puis.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• Substances dangereuses
De nombreux solvants organiques, phases mobiles et échantillons sont nuisibles à la
santé. Informez-vous de propriétés toxicologiques et infectieuses de toutes les substances
que vous utilisez. Les propriétés toxicologiques et infectieuses de nombreuses substances
peuvent être mal connues. Au moindre doute concernant une substance, traitez-la comme
s'il contenait une substance potentiellement dangereuse. Pour des instructions comment
utiliser correctement des composés particuliers, veuillez consulter à la fiche de données
des sécurités du fabricant respectif. Veuillez respecter des directives des Bonnes
Pratiques de Laboratoire (BPL).
Débarrassez-vous de tous les déchets de substances de manière écologique,
conformément à la règlementation en vigueur au niveau local. Empêchez impérativement
l'accumulation de solvants inflammables, toxiques et/ou infectieux. Suivez un
programme d'élimination des déchets règlementé et approuvé. Ne jetez jamais de
solvants inflammables, toxiques et/ou infectieux dans le système municipal d'évacuation
des eaux usées.
• Gaz dangereux
Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou
l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne
pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des
solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à
proximité.
• Décharge électrostatique
Décharge électrostatique peut provoquer la formation d'étincelles et peut présenter un
risque d’incendie. Veuillez noter que des solvants fluides dans les capillaires peuvent se
charger automatiquement. Cet effet se peut produire particulièrement forte dans les
capillaires isolants et avec des solvants non-conducteurs (par exemple, l'acetonitrile pur).
Prenez des mesures appropriées pour éviter les charges électrostatiques à proximité du
système HPLC. Par exemple, s'assurez qu'il y a une humidité de l'air suffisante et une
ventilation adéquate dans le laboratoire, portez des vêtements ou équipement de
protection antistatique, évitez l'accumulation de bulles d'air dans les lignes de déchets et
utilisez des réservoirs à déchets mis à la terre.
Utilisez uniquement des capillaires non-conducteurs pour diriger solvants au réservoir de
déchets. Capillaires électriquement conducteur devrait être mis à la terre.
Applications en phase normale:
N'effectuez pas d'applications en phase normale sans vous être assuré que votre faiseur
des gouttes est proprement isolée et mise à la terre. Les solvants de la phase normale,
non-conducteurs et non-polaires, par exemple hexane, peuvent entraîner une
accumulation de charges électrostatiques sur le faiseur des gouttes en l’absence de mise à
la terre. Le décharge électrostatique peut représenter un risque d’incendie et/ou
d'explosion.
Operating Instructions Page 7
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Notez que un faiseur des gouttes isolée et mise à la terre est disponible pour applications
en phase normale (référence 6702.0400). Utilisez les solvants de type phase normale
uniquement avec le collecteur de fraction AFC-3000 modifié à l’aide du cette faiseur des
gouttes isolée et mise à la terre. L'utilisation du collecteur de fraction sans le faiseur
des gouttes isolée et mise à la terre peut presenter un risque d'incendie et/ou
d'explosion.
• Inflammation spontanée des solvants
N’utilisez aucun solvants avec une température d‘auto-inflammabilité inférieure à
150° C. Si une fuite se produit, ces solvants peuvent s’auto-enflammer au contact d’une
surface chaude.
• Capillaires, connecteur capillaires, connexions ouvertes
♦ Des capillaires, en particulier les capillaires non-métalliques, pourraient fendre ou
glisser des connecteurs ou ne peuvent pas être vissés. Ceci peut en résulter aussi que
des substances pourraient jaillir des connexions ouvertes.
♦ Dans un système UltiMate 3000, certaines composantes sont en PEEK. Bien que ce
polymère présente une excellente résistance chimique à la plupart des solvants
organiques, il a tendance à gonfler lorsqu'il est en contact prolongé avec du
chloroforme (CHCl3), du diméthyle sulfoxide (DMSO) ou du tetrahydrofuran (THF).
De plus, il est attaqué par des acides concentrés tels que l'acide sulfurique et l'acide
nitrique ou d'un composé du hexane, éthyle acétate et méthanol. Ceci peut causer des
capillaires de fuite ou risquer des capillaires d’éclater. Ces acides peuvent cependant
être utilisés dans le cadre de procédures de nettoyage, à condition que l’exposition soit
brève.
♦ N'utilisez pas de capillaires écrasés, pliés, abimés ou endommagés.
♦ Les connecteurs capillaires pour pourrait être contaminé par des substances
dangereuses ou des substances dangereuses pourrait sortir des connexions ouvertes.
♦ Portez des lunettes de protection lorsque vous manipulez des capillaires en silice
fondue (pendant l'installation, découpe, etc.).
• Quand les capots de protection de l’appareil sont démontés, vous êtes exposés à des
connexions électriques sous haute tension deviennent accessibles. Débranchez
l'instrument de toute source d'alimentation électrique avant de retirer les capots. Ne
démontez les capots de protection que si cela est explicitement demandé au cours de ces
instructions. Les capots de protection devraient être démontés uniquement par le
personnel de service de Thermo Fisher Scientific.
• Remplacez les câbles de communication défectueux.
• Remplacez les cordons d'alimentation électrique défectueux. Utilisez uniquement les
cordons d’alimentation électrique spécifique à l’instrument.
• Utilisez seulement des pièces de rechange originales et des accessoires autorisés par
Thermo Fisher Scientific.
Page 8 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• Réglez toujours une limite de pression minimum pour la pompe HPLC. Ceci prévient les
dommages résultant de fuites ou de long-terme fonctionnement à sec de la pompe.
• Le collecteur de fraction pèse plus de 8 kg (17.7 livres). Par conséquent, soulevez le
collecteur de fraction avec précaution.
• Lorsque vous soulevez ou déplacez le collecteur de fraction, soulevez toujours par le bas
ou les côtés, afin de ne pas endommager l'instrument.
• Après utilisation, purgez le système des tampons et des susceptibles de former des
peroxydes.
• Lorsque vous passez d’une solution saline à un solvant organique, effectuez un rinçage
intermédiaire du système HPLC à l'eau dé-ionisée ou qualité HPLC.
• Lorsque vous passez à un autre solvant, assurez-vous que le nouveau solvant soit
miscible avec celui qui se trouve dans la pompe. Dans le cas contraire, la pompe peut être
endommagée; par exemple, par des floculations!
• Si une fuite se produit, arrêtez immédiatement l’instrument, stoppez le débit de la pompe
et remédiez au problème.
• Utilisez uniquement des solvants (qualité HPLC) et des solutions salines compatibles
avec les matériaux exposés phase mobiles.
• Afin d'éviter des blessures corporelles, ne mettez pas la main à l'intérieur du
compartiment à échantillons lorsqu'une analyse est en cours.
• Avant d'interrompre le fonctionnement pendant plusieurs jours ou plus, observez les
précautions figurant en page Fehler! Textmarke nicht definiert..
• N'utilisez pas l'instrument de manière autre que celles décrites dans ce manuel.
• Conservez ce manuel á proximité de l’instrument pour pouvoir le consulter facilement.
Operating Instructions Page 9
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
1.3 Intended Use
For Research Use Only. Not for use in diagnostic procedures.
The device is designed to be operated only be qualified and authorized personnel. All users
must know the hazards presented by the device and the used substances.
The fraction collector is designed for laboratory research use in high-performance liquid
chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC)
applications. The fraction collector is part of the UltiMate 3000 system, but can also be
used with other systems. A control computer with RS-232 or USB port is required.
The fraction collector is controlled by the Chromeleon Chromatography Management
System.
Observe the following for using the fraction collector:
• The fraction collector has been designed for the multidimensional separation of
biomolecules with aqueous and polar organic mobile phases, for example methanol, that
are commonly used in reversed-phase (RP) liquid chromatography. For normal-phase (NP)
applications, observe the safety instructions for the fraction collector on page 4. For the
safety instructions in French, see page 7.
• The fraction collector may be operated only using the components originally supplied with
the unit and within the technical specifications and environmental conditions (→ page 71)
that are outlined in this manual.
• Use only standard solvents (HPLC grade, preferably LC-MS grade (0.2 µm filtered)) and
buffers, compatible with the flow path materials. Note any special properties of the
solvents such as viscosity, boiling point, UV absorption (UV/VIS detector), refractive
index (refractive index detector), and dissolved gas (degasser).
If there is any question regarding appropriate usage, contact Thermo Fisher Scientific before
proceeding.
Warning: If the device is used in a manner not specified by Thermo Fisher
Scientific, the protection provided by the device could be impaired.
Thermo Fisher Scientific assumes no responsibility and will not be
liable for operator injury and/or instrument damage. Whenever it is
likely that the protection is impaired, the instrument must be
disconnected from all power sources and be secured against any
intended operation.
Page 10 Operating Instructions
Page 17
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Avertissement: Si l'instrument est utilisé de façon non spécifiée par Thermo Fisher
Scientific, la protection prévue par l'instrument pourrait être altérée.
Thermo Fisher Scientific n'assume aucune responsabilité et ne sera
pas responsable des blessures de l'operateur et/ou des dommages de
l'instrument. Si la protection de l'instrument n'est pas garanti à tout
moment, débranchez l'instrument de toutes les sources d'alimentation
électrique et assurez-vous que l'instrument n'est pas utilisé
involontairement.
1.4 Federal Communications Commission (FCC) Note
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference, in which case the user will be required to
correct the interference at his expense.
Operating Instructions Page 11
Page 18
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Page 12 Operating Instructions
Page 19
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2 Overview
2.1 Unit Description
The fraction collector can be operated with the UltiMate 3000 or any other (U)HPLC
system. The design has been optimized for robust and easy use as well as for minimized
carry-over volume. A wide range of fraction collection formats and a flow range up to
150 mL/min guarantees a maximum of flexibility.
• A universal drip tray accommodates a large variety of fraction collection racks and collects
spilled solvent. For detailed information about the supported rack types
see section 2.6 (→ page 20).
• The universal drip tray includes an integrated drain at its front left corner to guide spilled
solvents into a waste container.
• The diverter valve is mounted to a height-adjustable plate to allow closest possible
positioning over a fraction collection tube and to cut down the carry-over volume to a
minimum.
• A field-upgradable grounded drop former is available for normal-phase (NP) applications.
• The fraction collector is designed for easy access to the fluidic components, allowing fast
and reliable maintenance.
• All parts that may be exposed to solvents are made of materials that provide optimum
resistance to the most commonly used solvents and buffer solutions.
• The fraction collector is controlled by the Chromeleon Chromatography Management
System.
• Connection of the fraction collector to the Chromeleon computer is achieved by using
either a RS-232 serial or USB cable.
Operating Instructions Page 13
Page 20
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2.2 Operating Principle
The AFC-3000 Automated Fraction Collector collects fractions from the UltiMate 3000 or
any other HPLC system. A solenoid diverter valve directs the flow either to the waste or to
the fraction collection tubes. When Chromeleon sends a trigger signal to the AFC-3000
calling for fractions to be collected, the diverter valve moves over the assigned collection
tube and is then switched to route eluent flow into the tube.
During any movements, all tubes remain stationary while the moving diverter valve assembly
positions the drop former over the appropriate tube or above the rinse station.
The fraction collector includes the following fluidic components:
Component Description
Sample inlet capillary Eluent is supplied from the detector outlet to the common (inlet) port of the
diverter valve through this tubing.
Diverter valve This valve directs the eluent from the detector to either waste or to a collection
tube.
Drop former This component is attached to the diverter valve and forms droplets or a jet of
eluent for collection into tubes.
Waste tubing This tubing connects the waste outlet port of the diverter valve to a waste
container or to a drainage system.
Rinse station A tube-like shaped port, which is mounted to the head of the fraction collector.
The outlet of this station is connected to a waste container or a drainage
system through a tubing.
Collection tube The tube into which eluent/sample is collected.
The fraction collector includes the following axis components:
Axis Component Description
X When facing the front of the unit, the left to right movement axis of the
fraction collector arm.
Y When facing the front of the unit, the fore and aft movement axis of the
diverter valve assembly on the fraction collector arm.
Z The manually adjustable height axis of the diverter valve assembly.
Page 14 Operating Instructions
Page 21
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2.3 Fraction Collector Configurations
The fraction collector is available in the following standard configuration for flow rates
up to 150 mL/min:
Description Part No.
AFC-3000 Automated Fraction Collector, including:
5702.1000
- two 90 position racks
- 180 x 8 mL tubes, 13 mm OD
- 1.6 mm ID diverter valve and 1 mm ID PEEK drop former
Kit for low flow rates
A kit for flow rates up to 10 mL/min is available. It includes a different diverter valve, which
replaces the standard valve.
Description Part No.
Kit for low flow rates, including:
6702.0300
1.0 mm ID diverter valve with 0.4 mm ID PEEK drop former
Kit for normal-phase LC
In addition to the standard and low-flow PEEK drop formers, Thermo Fisher Scientific
offers a stainless steel drop former with 1.0 mm ID for use with normal-phase solvents.
The stainless steel drop former provides grounding via the diverter valve cable and is
suitable for flow rates up to 150 mL/min.
Description Part No.
Kit for normal-phase LC, including:
6702.0400
1.0 mm ID stainless steel drop former with grounding cable
Warning: Do not perform normal-phase applications without a proper
grounding protection of the drop former. Non-conductive, non-polar
normal-phase eluents, for example hexane, can lead to a build-up of
electrostatic charges on an ungrounded drop former. The
electrostatic discharge can create a fire and/or explosion hazard.
Operating Instructions Page 15
Page 22
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Avertissement: N'effectuez pas d'applications en phase normale sans vous être assuré
que votre faiseur des gouttes est proprement isolée et mise à la terre.
Les solvants de la phase normale, non-conducteurs et non-polaires,
par exemple hexane, peuvent entraîner une accumulation de charges
électrostatiques sur le faiseur des gouttes en l’absence de mise à la
terre. Le décharge électrostatique peut représenter un risque
d’incendie et/ou d'explosion.
For information about the optional accessories that are available for the fraction collector,
see section 9.2 (→ page 74).
Page 16 Operating Instructions
Page 23
4 3 1 6 2
5
2.4 Front Panel Elements
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Fig. 1: Front panel view
No. Front Panel Element Description
1 Power indicator LED The LED is illuminated blue when the fraction collector is turned on.
2 Fraction collector arm
3 Diverter valve assembly Assembly to which the diverter valve is attached. The mounting
4 X travel slot Front cover opening which facilitates travel of the fraction collector
5 Rinse station
6 Universal drip tray with
drain
Transports the diverter valve assembly fore-and-aft (→ see also
section 2.2, page 14).
height of the diverter valve assembly is manually adjustable to attain
the proper clearance above the collection tubes and rinse station.
arm in the right-to-left, or ‘X’ direction (→ see also section 2.2,
page 14).
The tube shaped port, mounted to the cabinet of the fraction
collector, where eluent may be dispensed and delivered to the waste.
The outlet of the rinse station is connected to a waste container or
drainage system.
Drip tray which allows mounting of supported rack types. The tray
guides spilled solvents into a waste container.
Operating Instructions Page 17
Page 24
UltiMate 3000 Series:
1 2 3
4
5
6
7
8
AFC-3000 Automated Fraction Collector
2.5 Rear Panel
Fig. 2: Rear panel view
No. Description
1 Type label
2 Auxiliary I/O connection
Connects to diverter valve assembly to provide power to the diverter valve and grounding to
the stainless steel drop former (if equipped).
3 Power switch
4 Main power receptacle
5 Serial communication port (unused)
6 USB port (Universal Serial Bus) for connection to the Chromeleon computer
7 COM 1 RS-232 port (serial) for connection to the Chromeleon computer
8 Mounting block of diverter valve assembly
Page 18 Operating Instructions
Page 25
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2.5.1 Power Switch
The power switch on the rear panel is the main power switch for the fraction collector.
Turn on the power switch before initial operation of the fraction collector. Turn off the
main power switch when instructed to do so, for example, before performing a service
procedure or when interrupting operation for longer time periods. In this case, also observe
the safety precautions outlined in this manual.
2.5.2 USB Port
You can use a USB connection to connect the fraction collector to the Chromeleon
computer, for example, if no RS-232 COM port is available. The communication data is
transferred digitally via the appropriate USB cable. However, a USB converter software
must be installed so that Chromeleon can communicate with the fraction collector. To
ensure trouble-free operation, all USB cables must be ordered from the Thermo Fisher
Scientific sales organization for Dionex HPLC Products.
For information about how to connect the fraction collector to the Chromeleon computer, see
section 3.4 (→ page 35).
2.5.3 RS-232 (Serial) Port
The Chromeleon Chromatography Management System uses a serial connection (RS-232)
to control the fraction collector. The communication data is transferred digitally via the
appropriate RS-232 cable. To ensure trouble-free operation, all RS-232 cables must be
ordered from the Thermo Fisher Scientific sales organization for Dionex HPLC Products.
2.5.4 Auxiliary I/O Connection
The auxiliary I/O connection is used to provide power to the diverter valve and grounding
to the stainless steel drop former. The appropriate cable is pre-installed on the diverter
valve.
Operating Instructions Page 19
Page 26
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2.6 Universal Fraction Tray, Racks and Tubes
The fraction collector supports a large variety of fraction collection racks, including most
of the Isco Foxy Jr./R1 and wellplate sampler racks. For information about how to install a
rack, see page 30.
The following rack types are supported:
Rack/Adapter for Tube Size Part No.
21 tubes 50 mL; 30 mm x 100 mm
A funnel rack (part no. 6702.1021) in conjunction with
this rack allows collecting fractions of unlimited
volumes into appropriate vessels.
24 tubes 30 mL; 24 mm x 100 mm 6702.0024
40 tubes 20 mL; 20 mm x 100 mm 6702.0040
60 tubes 14 mL; 16 mm x 100 mm 6702.0060
90 tubes 8 mL; 13 mm x 100 mm 6702.0090
60 tubes (Foxy Jr./R1) 1.5 mL 5701.2023
72 tubes (Foxy Jr./R1) Mini tubes, 18 mm OD 5701.2024
36 vials (Foxy Jr./R1) Scintillation vials, 28 mm OD 5701.2025
2 well plates (Foxy Jr./R1) Two 96 well plates 5701.2021
40 vials (WPS-3000) 2 mL, 12 mm OD
In addition, a rack adapter (part no. 6702.0100) is
required. The vials should have an opening as large as
possible for unerring fractionation.
22 vials (WPS-3000) 4 mL, 15 mm OD
In addition, a rack adapter (part no. 6702.0100) is
required.
6702.0021
6820.4070
6820.4084
10 vials (WPS-3000) 10 mL, 22 mm OD
In addition, a rack adapter (part no. 6702.0100) is
required.
4 well plates Four 96 well plates 6702.0200
6820.4086
In addition, up to six 250 mL Schott bottles (part no. 2270.0026) can be placed directly on
the universal drip tray.
To ensure correct installation of the Foxy racks, a set of positioning pins is available from
Thermo Fisher Scientific (part no. 6702.9006).
For details on rack installation and allowed combinations of fraction formats, see section
3.3.5, page 30.
Page 20 Operating Instructions
Page 27
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2.7 Chromeleon Software
The fraction collector can exclusively be controlled by the Chromeleon Chromatography
Management System. For operating the fraction collector with Chromeleon, the following
licenses are required:
• Timebase Class 1
• Fraction Collection (part no. 5960.0038)
for basic fraction collection, with wizard-based program setup and fraction reporting.
—or—
• Extended Fraction Collection (part no. 5960.0039)
for advanced fraction collection. The Extended Fraction Collection license includes the
Fraction Collection license. In addition, it supports functions for advanced fraction
collection, such as automatic sample purification via the associated post-acquisition steps,
color-coded sample and fraction tracking, and sophisticated fractionation algorithms with
peak shoulder detection.
For more information about the Chromeleon licenses, contact the Thermo Fisher Scientific
sales organization for Dionex HPLC Products.
Two modes of software control are available:
• Direct Control
With direct control, you select operating parameters and commands in the Chromeleon
Commands (F8) dialog box. Direct commands are executed as soon as they are entered.
For routine operation, most parameters and commands are available also on a control
panel. For more information about direct control, see page 46.
• Automated Control
With automated control, you use a program file. This is a list of control commands,
executed in chronological order for automated operation of the fraction collector. For more
information about automated control, see page 52.
Operating Instructions Page 21
Page 28
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Page 22 Operating Instructions
Page 29
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
3 Installation
3.1 Facility Requirements
The AFC-3000 operates reliably even under less than ideal conditions. It is not, however,
indestructible. Malfunction or damage can occur if specific operating conditions are not
met. Meeting these conditions requires that you create a proper lab environment, replace
fraction collector components that wear out under normal use, and purchase the
appropriate supplies for use with the fraction collector.
Important: Damage or malfunction that results from unsatisfactory operating
conditions may constitute misuse or abuse and may therefore be
excluded from warranty coverage.
Important: Dommage ou de dysfonctionnement qui résulte de conditions
d'exploitation insatisfaisantes peut constituer une utilisation abusive
ou l'abus et être exclu de garantie.
The installation site must meet the following requirements:
• The main power switch and the main power receptacle are on the rear panel. Make sure
that
♦ Free and unrestricted access to the main power switch is ensured at all times.
♦ The power cord of the device can be easily reached and disconnected from the power
line at all times. Provide sufficient space behind the device to unplug the cable.
• Make sure that the installation site meets the power and environmental specifications listed
in the Technical Information section (→ page 71).
• Install the instrument in the laboratory on a stable surface that is free of vibrations.
• Avoid rough handling of the AFC-3000. Do not expose the fraction collector to vibration
or shock.
• Make sure that the surface is resistant to solvents.
• Avoid locations with extreme changes in temperature (for example, caused by direct
sunlight or drafts) and high humidity.
• Allow sufficient clearance behind and on the sides of the fraction collector for power
connections and ventilation.
• Make sure the unit is not exposed to excessive flammable or corrosive materials.
• Protect the fraction collector from long-term exposure to condensation, corrosive
materials, solvent vapor, continual standing liquids, or large spills into the fraction
collector cabinet or fraction collector arm. Exposures of this type can damage the drive
mechanisms as well as the electronics.
Operating Instructions Page 23
Page 30
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• Observe the same general electrostatic discharge precautions as with any other integrated
circuit electronic devices. Low-humidity environments, especially when combined with
static-generating materials, require maximum care.
Important: Discharge static buildup and ground to the fraction collector base or
cabinet before performing any maintenance. Do not touch or
short-circuit bare contacts (for example, COM1 or auxiliary ports).
Important: Déchargez accumulation d'électricité statique et mettez à la terre la
base et le cabinet du collecteur de fractions avant de procéder à tous
travaux d’entretien. Ne touchez ou court-circuitez pas des contacts
nus (par exemple, COM1 ou ports auxiliaires).
• Avoid using the AFC-3000 if strong electromagnetic interference, radio frequency
interference, or radioactivity is present. Interference fields can cause erratic operation of
the fraction collector. The fraction collector will not function properly if the level of
radioactivity is above background.
3.2 Unpacking
All electrical and mechanical components of the fraction collector are carefully tested
before the instrument is shipped from the factory. After unpacking, please inspect the
instrument for any signs of mechanical damage that might have occurred during transit.
Tips: Immediately report any shipping damage to both, the incoming
carrier and Thermo Fisher Scientific. Shipping insurance will
compensate for the damage only if reported immediately.
Keep the original shipping container and packing material. They
provide excellent protection for the instrument in case of future
transit. The product warranty will not be honored if the fraction
collector is shipped in any other packaging.
Important: Ship the unit only in the original shipping container and observe the
packing instructions. Shipping the unit in any other packaging
automatically voids the warranty. For more information, see the
warranty statement in the terms of sale.
Important: Expédier l'unité uniquement dans le conteneur d'expédition original
et d'observer les instructions d'emballage. L'unité dans toute autre
emballage automatiquement d'expédition, la garantie en vides. Pour
plus d'informations, consultez la déclaration de garantie dans les
conditions de vente.
Page 24 Operating Instructions
Page 31
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
1. Place the shipping container on the floor and remove any accessories.
2. Grasp the fraction collector by the sides. Slowly and carefully, pull the instrument out
of the shipping container and place it on a stable surface.
Important: The fraction collector weighs more than 8 kg (17.7 lbs). Therefore,
you should use caution when lifting the fraction collector. To prevent
the unit from falling, always lift by the bottom or the sides of the
unit. Do not lift the fraction collector by the packing material.
Important: Le collecteur de fraction pèse plus de 8 kg (17.7 livres). Par
conséquent, soulevez le collecteur de fraction avec précaution. Afin
d'empêcher l'instrument de tomber, saisissez-la par le bas ou les
côtés de l'unité. Ne soulevez l'instrument à l’aide du matériau
d'emballage.
3. The fraction collector is encased in protective wrap and is fitted with form-fitting foam
spacers, which allow for secure placement and positioning inside the shipping box.
Remove the foam spacers, and then remove the protective wrap.
4. Before connecting the fraction collector to the power source, wait 4-8 hours to allow
the instrument to adapt to room temperature and to allow any condensation that might
have occurred during shipping to evaporate. After 4-8 hours, check the fraction
collector; if condensation still exists, allow the fraction collector to continue to warm
up (without connecting it to the power source) until any condensation is completely
gone.
Operating Instructions Page 25
Page 32
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
3.3 Installing the Fraction Collector
The AFC-3000 is designed for easy installation consisting of two parts; assembling the
fraction collector and connecting it to the analytical instrument.
For the most part, you can install the AFC-3000 without using tools. In fact, using tools such
as screwdrivers or pliers to perform most installation tasks may result in a damaged or
unusable instrument. You can remove the knurled screws with tools if necessary, but do not
tighten them with anything other than your fingers.
To install the fraction collector, you must complete the following tasks (each of these tasks
will be discussed in detail later in this section):
• Mount the diverter valve assembly (→ page 27)
• Establish connections on the diverter valve (→ page 28)
• Attach the rinse station (→ page 29)
• Connect the rinse station tubing (→ page 29)
• Check the proper alignment of the universal drip tray
• Place the fraction tube racks on the tray (→ page 30)
• Set the drop former height adjustment (→ page 33)
• Establish external connections (→ page 35)
• Set up the fraction collector in Chromeleon (→ page 39)
Important: Ensure that the power is off before proceeding with installation.
Important: Assurez-vous que l'alimentation secteur est éteint avant de procéder
à l'installation.
Page 26 Operating Instructions
Page 33
UltiMate 3000 Series:
Knurled screws
AFC-3000 Automated Fraction Collector
3.3.1 Mounting the Diverter Valve Assembly
Mounting the diverter valve assembly on the fraction collector arm is the first major task.
The diverter valve assembly attaches to the fraction collector arm using two knurled
finger-tight screws.
1. Place the diverter valve assembly on the fraction collector arm and mount it with two
knurled finger-tight screws. No tools are required to fasten the screws. Fig. 3 shows
the assembly attached.
Fig. 3: Diverter valve assembly
2. Attach the mounting block to the AFC-3000 rear panel with the finger-tight mounting
block screws provided (→ Fig. 4).
Fig. 4: Attaching the mounting block
3. Connect the diverter valve cable to the auxiliary I/O connection on the AFC-3000 rear
panel (→ Fig. 2, page 18). Tighten the knurled nut finger-tight.
4. Screw the PEEK drop former finger-tight into the diverter valve.
Operating Instructions Page 27
Page 34
UltiMate 3000 Series:
Sample inlet
Waste
AFC-3000 Automated Fraction Collector
3.3.2 Diverter Valve Connections
When connecting capillaries to the fraction collector, observe the following general
precautions:
• Observe the precautionary statements for capillaries and capillary connections in
section 1.2.2 (→ page 3).
• When you connect capillaries, make sure that the connectors are free from contaminants.
Even minute particles may cause damage to the system.
• Use only the capillaries shipped with the fraction collector and original Dionex spare
capillaries.
Connect the waste and sample inlet tubing to the diverter valve. Verify that the tubings are
properly connected and tightened.
Fig. 5: Connections on the diverter valve
Note the following when connecting the sample inlet:
In order to make sure that the capillary end is not pushed into the valve bore, place the ferrule
at the capillary end so that the capillary does not protrude from the ferrule (→ Fig. 6). Then
carefully install the capillary.
right wrong
Fig. 6: Connecting the sample inlet tubing to the diverter valve
Page 28 Operating Instructions
Page 35
UltiMate 3000 Series:
Elbow piece
Drain tubing
Rinse station
Tray drain
AFC-3000 Automated Fraction Collector
3.3.3 Attaching the Rinse Station
The rinse station is located at the extreme left position of the fraction collector head
(→ Fig. 1, page 17). This station is used to dispense liquid to waste through normal
operation of the HPLC system operation. The dispensed liquid is evacuated by gravity
drain via a gravity drain arrangement into a waste reservoir.
The rinse station is installed in its holding bracket at shipment, but can be removed easily by
pulling the station out of the bracket. The rinse station easily “snaps” back into place.
Tip: Ensure that the height of the drop former and rinse station is properly adjusted
to avoid crashes (→ section 3.3.6, page 33 and section 4, page 34).
3.3.4 Connecting the Drain Tubing
The rinse station drain tubing attaches to the rinse station. Use an elbow piece (shipped
with the fraction collector) to connect the drain tubing to the rinse station (→ Fig. 7).
Connect another drain tubing in the same way to the integrated drain at the front left corner
of the drip tray. You can use the Y piece shipped with the fraction collector to connect the
two drain tubes to a single drain. You can also connect them to an UltiMate 3000 drain
system, if applicable. The tubing should be routed to an appropriate waste container or
drainage system.
Fig. 7: Connection of the drain tubing
Place the waste container below the fraction collector to allow any liquids to flow off.
Ensure that the free end of the tubing inside the waste container is always above the liquid
level. If necessary, shorten the tubing.
Operating Instructions Page 29
Page 36
UltiMate 3000 Series:
Universal drip
tray
Rack
AFC-3000 Automated Fraction Collector
Fig. 8: Rinse station tubing in waste container
3.3.5 Placing Fraction Racks on the Universal Drip Tray
The fraction tube racks in the AFC-3000 accessories kit are shipped unassembled. First,
assemble the racks using the supplied instructions. Then, position them on the universal
drip tray, using care to line up the rack locating pins with the associated recesses in the tray
(Fig.9).
Other rack types such as Thermo Scientific Dionex well-plate sampler racks and the 4 x 96
well plates require special adapters which can be ordered separately. The adapter block is
correctly installed on the drip tray in one position only, when the recess on the bottom of the
adapter block is properly aligned with the elevated parts of the drip tray.
Page 30 Operating Instructions
Fig.9: Racks placed in universal drip tray
Page 37
UltiMate 3000 Series:
1
Rack 2
AFC-3000 Automated Fraction Collector
Important: Before loading or unloading any fraction racks on the universal drip
tray, move the arm with the drop former to the home position by
cycling the power off and on (or by sending the RinsePosition
comand). Never attempt to load, unload, or reposition a fraction rack
or fraction tube while the fraction collector is operating.
Important: Avant de charger ou décharger aucun des racks de tubes de fraction
du plateau de fractions, parquez le bras avec le générateur des
gouttes en position d’origine par eteindre et allumer consécutivement
l'instrument (ou donner la RinsePosition commande). Jamais tentez
charger, décharger ou repositionner un des racks de tubes de fraction
ou un tube de fraction tandis que le collecteur de fraction est en
operation.
When installing funnel racks on the 21-position rack, connect tubing (shipped with the
rack) to each funnel and route the tubing to an appropriate container or bottle. Observe the
instructions for drain tubing (→ page 29).
Depending on the rack type, you can install a certain number of racks, deep well plates, or
bottles. The maximum number of racks and the order in which the racks must be placed
and configured in Chromeleon can be taken from table on page 31. The rack layout is
shown facing the front of the unit. Note that it is not allowed to install a rack, for example,
in position 2 if no rack is installed in position 1. For details on setting the racks in
Chromeleon, refer to section 5.2.3 (→ page 49).
Fraction rack Maximum number of racks/bottles Rack layout
21Pos, 24Pos, 40Pos,
60Pos, 90Pos
36Pos_Foxy,
60Pos_Foxy,
72Pos_Foxy
Two racks can be installed on the universal
drip tray, holding the specified number of
tubes. Any two racks may be used together at
one time.
One Foxy rack can be installed on the
universal drip tray, holding the specified
number of tubes.
96Pos_Foxy Two well plates (96 positions) can be installed
on the Foxy well plate holder.
Operating Instructions Page 31
Page 38
UltiMate 3000 Series:
Positioning pins
AFC-3000 Automated Fraction Collector
Fraction rack Maximum number of racks/bottles Rack layout
96Pos Four well plates (96 positions) can be installed
on the well plate adapter. Well plates must not
be mixed with other rack types.
10Pos_WPS,
22Pos_WPS,
40Pos_WPS
Bottle Six 250 mL Schott bottles can be placed
Four WPS-3000 well-plate sampler racks can
be installed on the rack adapter. Any four
racks may be used together at one time.
directly on the universal drip tray. You must
always install all six bottles. It is not possible
to mix bottles with other rack types.
Foxy rack positioning pins
When Foxy Jr./R1 racks are used, Thermo Fisher Scientific recommends installing two
positioning pins (part no. 6702.9006) to prevent incorrect installation and orientation of a
Foxy rack. Place the pins in the holes provided in the universal drip tray. With the pins
installed, a Foxy rack cannot be installed incorrectly on the universal drip tray.
Page 32 Operating Instructions
Fig. 10: Positioning pins for Foxy racks
Page 39
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Tip: To prevent the universal drip tray from accidentally being lifted out of its
proper position, always remove the rack adapter toward the side as shown here:
Fig. 11: Removing the rack adapter
3.3.6 Adjusting the Drop Former Height
1. Grasp the diverter valve assembly firmly by the vertical stainless steel element while
rotating the adjustment locking lever upward. This will unlock the slider assembly,
allowing you to move it up and down freely (→ Fig. 12).
2. Slide the assembly so that the drop former is several millimeters above the highest
point of the fraction tubes or vials. Adjust as needed.
3. Grasp the diverter valve assembly firmly by the vertical stainless steel element while
rotating the adjustment locking lever downward. This will lock the diverter valve
assembly so that its height is fixed. If the lever cannot be moved downward easily, the
diverter valve assembly may not be positioned properly in one of the grooves.
Operating Instructions Page 33
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UltiMate 3000 Series:
Diverter valve
Drop former
Adjustment
locking lever
AFC-3000 Automated Fraction Collector
Fig. 12: Diverter valve assembly with drop former
4. Slide the rinse station up or down in its holder so that the lowest part of the drop
former is slightly above the top of the rinse station when it is in the rinse position.
Tip: This setting ensures that the diverter valve assembly will not impinge on
the rinse station during movement, while allowing for the minimum
distance between those two components to minimize the risk of eluent
being dispensed outside of the rinse station barrel. In case of an
obstruction during the movement of the drop former, the device will turn
off after ca. 20 seconds to avoid damage.
5. Turn the fraction collector off and manually move the diverter valve assembly to the
four corner positions of the installed fraction racks. This is to verify that the adjusted
height is suitable for all positions despite any tolerances that may exist.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
3.4 Connecting the Fraction Collector
The next steps in the installation process involve connecting the AFC-3000 to the power
source and to the Chromeleon computer. The following sections explain how to establish
these connections.
3.4.1 Connecting the External Desktop Power Supply
A voltage-specific external desktop power supply is delivered with each AFC-3000.
Use the external desktop power supply shipped with the fraction collector to connect the
instrument to the main power source:
1. Place the AFC-3000 within 1.2 meters of a power outlet.
2. Connect the external desktop power supply to the DC power input connector on the
rear panel of the fraction collector (→ Fig. 3, page 19).
3. Connect the supply side of the power supply to the power source that is connected to a
true ground.
The input rating is AC 100V-240V 1.9 A with an output of DC 24V, maximum 3.33 A. No
manual adjustment is required to adapt the line voltage to local voltage requirements.
Warning: Never use a power cord other than the power cords provided for the
device.
Do not use multiple sockets or extension cords. Using defective
multiple sockets or extension cords may cause personal injury or
damage to the device.
Avertissement: Utilisez uniquement les cordons d’alimentation électrique spécifique
à l’instrument.
N'utilisez pas des blocs multiprise ou des câbles prolongateurs. Cela
pourrait entraîner des blessures corporelles ou endommager
l'instrument.
Important: The AFC-3000 is intended to operate from an AC power source that
will not apply more than 240V AC between the supply conductors
and ground. A protective ground connection by way of the
grounding connector in the power cord is required for safe operation.
Important: L'AFC-3000 est destiné à fonctionner d'une source d'alimentation
courant alternatif qui s'appliquera pas plus que 240V courant
alternatif entre le conducteur et la terre. Une connexion de protection
à la terre par le fil neutre dans le fil d'alimentation est nécessaire
pour la sécurité d'exploitation.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
3.4.2 General Communications Information
To connect the fraction collector to the Chromeleon computer, select one of the following
alternatives:
• Use an RS-232 cable (part no. 8914.0153, shipped with the instrument) to connect the
COM 1 port on the rear panel of the fraction collector to a serial COM port on the
computer (→ section 3.4.3, page 36).
• Use a USB cable (part no. 6911.0002, shipped with the instrument) to connect the USB
port on the rear panel of the fraction collector to a USB port on the computer.
To use USB, a virtual COM port has to be installed to the Chromeleon computer
(→ section 3.4.4, page 36).
Tip: When interconnecting any computing devices, keep the communications cables
away from sources of electromagnetic or radio frequency (RF) interference,
such as electric motors, transformers, fluorescent light ballasts, or RF energy
sources.
3.4.3 Communication via RS-232
To establish connection via a serial (RS-232) cable:
1. Connect the cable to COM 1 serial (RS-232) port on the rear panel of the fraction
collector (→ Fig. 2).
2. Connect the cable to a serial (RS-232) port on the Chromeleon computer
3.4.4 Communication via USB
Before you can operate the fraction collector with Chromeleon via USB, an additional
virtual COM port must be created on the Chromeleon computer. Select this new COM port
when configuring the fraction collector in Chromeleon (→ page 41).
The required drivers are usually found and installed automatically when the fraction
collector is plugged in. If this is not the case, complete the following steps:
Installing the Virtual COM Port on the Chromeleon Computer
1. Locate the Drivers\USB Virtual COM Port folder on the Chromeleon Service
Release DVD.
2. Check which files are contained in the folder (the shipped driver version depends on
the Chromeleon version):
• The folder contains the CDM 2.06.00.exe file (or a later version): Double-click the
file. This will install the drivers.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• The folder contains the 2.08.24 folder (or a higher number) and further sub folders
and PDF files: The installation instructions (PDF file) for the drivers can be found in
this folder. Install the drivers as described in the instructions for your operating
system.
Connecting a USB Cable
Tip: Verify that Chromeleon is installed on the computer and that the license code is
entered before you connect the fraction collector to the Chromeleon computer.
To establish a connection via a USB cable:
1. Turn on both the Chromeleon computer and the fraction collector.
2. Plug one end of the USB cable into the USB port on the Chromeleon computer and the
other end to the USB port on the fraction collector.
Tips: As an alternative, you can connect the fraction collector to the internal
USB port on another module in the UltiMate 3000 system that is
connected to the Chromeleon computer, or connect the fraction collector to
the Chromeleon computer via an external USB hub (→ Fig. 13, page 38).
The USB standard limits the USB cable length to 5 meters. Each USB
device can be separated from the computer or next USB hub by no more
than 5 meters.
3. The required drivers (USB Serial Converter and USB Serial Port) are usually found
and installed automatically (see below). If this is not the case, install the drivers
manually as described above under Installing the Virtual COM Port on the
Chromeleon Computer.
Windows Vista, Windows 7 and Windows Server 2008
will automatically detect the fraction collector and perform the USB installation. The
message "Your device is ready to use" appears. When you open the message, you can see
the number of the new virtual COM port (e.g., COM3).
Windows XP
will automatically detect the fraction collector and launch the Found New Hardware
Wizard, which guides you through the installation of the USB Serial Converter:
♦ If asked whether Windows can connect to Windows Update to search for software,
select Yes, this time only. If no internet connection is available, you must manually
install the drivers as described in section 2.2.
♦ Click Finish when the wizard reports that the software has been installed.
♦ The Found New Hardware Wizard is launched again. Repeat the same steps for
installation of the USB Serial Port.
Operating Instructions Page 37
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Fig. 13: Rear panel connections on an UltiMate 3000 system with AFC-3000 (Example)
Page 38 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
3.5 Setting Up the Fraction Collector in Chromeleon
This section provides brief instructions for installing and configuring the fraction collector
in Chromeleon.
Tip: The minimum requirement for control of the AFC-3000 is Chromeleon 6.80
SR7e (Driver Update).
3.5.1 Installing the Fraction Collector
1. Start the Chromeleon Server Monitor program by double-clicking the Chromeleon
Server Monitor icon on the Windows taskbar.
If the Server Monitor icon is not on the taskbar, click Start on the taskbar, point to
Programs (or All Programs, depending on the operating system), point to Chromeleon,
and then click Server Monitor.
2. Click Start to start the server.
3. Click Close to close the Chromeleon Server Monitor window. The Server Monitor
icon appears on the taskbar.
Tip: Clicking the Quit Monitor button in the Chromeleon Server Monitor
window quits (exits) the Server Monitor program, but does not stop the
server. To stop the server, click Stop.
4. Start the Chromeleon Server Configuration program by clicking Start on the taskbar.
Point to Programs (or All Programs, depending on the operating system), point to
Chromeleon, and then click Server Configuration.
5. If necessary, click the plus sign beside the server icon to display the items
underneath.
6. Select the timebase to which the fraction collector will be assigned, or create a new
timebase (on the Edit menu, click Add Timebase).
7. Open the Add device to timebase dialog box. To do so, click Add Device on the Edit
menu or right-click the timebase and click Add Device on the menu.
8. On the Manufacturers list, click Dionex and on the Devices list, click AFC-3000
Fraction Collector.
9. There are three configuration tab pages. Make the required settings on the first two tab
pages. For more details, see section 3.5.2.
10. Click OK to complete the installation of the fraction collector.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
11. Open the Add device to timebase dialog box again. On the Manufacturers list, click
Generic and on the Devices list, click Fraction Collection. Accept the settings on the
two configuration pages, then click OK.
12. On the File menu, click Save Installation and then close the Server Configuration
program.
3.5.2 Configuring the Fraction Collector
This section describes the settings that must be made on the first two configuration pages.
Dionex AFC-3000 Fraction Collector Page
On this page, you can activate or deactivate the Demo Mode.
Fig. 14: Dionex AFC-3000 Fraction Collector page
• Demo Mode
Verify that the check box is cleared. In the Demo Mode, Chromeleon simulates the
functions of the fraction collector. If the Demo Mode is enabled, the Port list will be
unavailable on the AFC page.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
AFC Page
This page is used to configure the connection between Chromeleon and the fraction
collector.
Fig. 15: AFC page
• Device Name
The name used to identify the fraction collector in the installation environment and in the
Chromeleon client program. To control the fraction collector with the existing control
panels, accept the default name. If you enter a different name, you may have to re-link the
controls on the control panels and edit the device name in the program files.
• Serial Communication
In the Port list, select the serial interface port to which the fraction collector is connected.
This is either the COM port on the Chromeleon computer to which the RS-232 serial cable
is connected, or, if a USB cable is used for the connection, the virtual COM port (USB
Seria Port) that was created during installation of the USB drivers (Windows automatically
allocates a number for the virtual COM port, for example, COM5; usually, this is the COM
port with the highest number). In addition, the current interface parameters are displayed.
The port selection list is unavailable when Chromeleon is in demo mode.
Operating Instructions Page 41
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Page 42 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
4 Verifying Installation
4.1 Overview of Actions
Once the installation of the AFC-3000 has been completed, it is important to verify the
correct installation to ensure proper functionality of the fraction collector.
Installation verification of the fraction collector consists of three parts:
• Visual inspection of all components
• Ensuring that the communications interface between the fraction collector and the
Chromeleon computer is working
• Ensuring that the diverter valve assembly functions properly
This chapter explains how to test the above items before you use the fraction collector.
4.2 Inspecting Components Prior to Fluidic Operation
Complete the following steps:
1. Shut down and unplug the fraction collector.
2. Visually inspect the drop former, rinse station and all attached tubing for leaks or signs
of damage.
If you detect a leak or other damage to a fraction collector component, you must replace the
component. For more information, see the Service section (→ page 61).
4.3 Testing the Interface
Test the communications interface between the fraction collector and the Chromeleon
computer.
1. Start the Chromeleon Server if it is not already running (→ page 39).
2. Verify that the cable is properly connected.
3. Start the Chromeleon client by clicking the Chromeleon icon .
If the Chromeleon icon is not on the desktop, click Start on the taskbar, point to
Programs (or All Programs, depending on the operating system), point to Chromeleon,
and then click Chromeleon.
4. Open the Chromeleon Browser and double-click the UltiMate_3000_AFC-3000.pan
control panel in the Dionex Templates/Panels/Dionex_LC folder.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5. Connect the control panel to the timebase in which the fraction collector is installed.
On the Control menu, select Connect to Timebase, and then select the timebase on
the Timebase tab. For information about the Timebase dialog, click Help.
6. Verify that the fraction collector is connected to Chromeleon. If it is not, click
Connect.
The fraction collector resets its position, with the diverter valve assembly moving in
defined in X- and Y-direction and back over the rinse position. If the fraction collector
does not reset, see the Troubleshooting section (→ page 59).
4.4 Testing the Drop Former Alignment
The drop former must align to the center of each fraction tube to ensure satisfactory sample
dispensing. Shipping or rough handling can disturb the fraction collector’s cabinet-to-base
alignment. If it is incorrectly aligned, the fraction collection into the tubes might be
disturbed. It is therefore important to test it before you actually run samples with the
fraction collector.
Tip: Before testing the alignment of the drop former, ensure that you have installed
all fraction collector components correctly. Also, ensure that you have securely
tightened all finger-tight screws.
Testing the drop former involves observing its operation. To do so, complete the following
steps:
1. Load the universal drip tray with the rack type and tubes intended to be used (→
section 3.3.5).
2. Turn the fraction collector power switch on and verify that the LED power indicator is
on (blue).
3. Set the correct rack configuration in Chromeleon (→ section 5.2.3, page 49).
4. In Chromeleon, open the Commands dialog box (→ page 46). Click the plus sign
beside AFC, and then click TubePosition.
5. In the TubePosition box, select a suitable position, for example, position 1, and click
Execute to move the drop former to the designated dispense position. Check that the
drop former tip correctly accesses each position at the center of the tube. Repeat for
further suitable positions as needed.
Tip: If the fraction collector alignment is not correct, contact Thermo Fisher
Scientific Service for Dionex HPLC Products.
Page 44 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5 Operation and Maintenance
The AFC-3000 is both reliable and easy to use. Before using it, however, ensure that your
lab environment provides operating conditions that will prolong the life of the AFC-3000.
When you finish using the fraction collector, you may need to flush the rinse station and
flow path before shutting the fraction collector down.
The AFC-3000 can be operated with the Chromeleon Chromatography Management System.
Stand-alone operation is not supported.
5.1 Power-Up
Once you arrange the tube racks and ensure that the arrangement is correctly defined in the
software, you can start the fraction collector and let it run until the sampling sequence is
finished. To do so, complete the following steps:
1. Ensure that the rinse station is properly connected (→ section 3.3.4, page 29).
2. Turn the AFC-3000 power switch on. The blue LED indicator along the fraction
collector x-axis lights up when the power is on.
3. Ensure the waste tubing is in the correct waste container and proper drain flow is
achieved.
4. Connect the AFC-3000 to Chromeleon (→ section 5.2.1).
5.2 Operation with Chromeleon
Before you begin, verify that
1. The Chromeleon software is installed on the computer and the license code is entered.
The minimum requirement for control of the AFC-3000 is Chromeleon 6.80 SR7e,
(Driver Update). If a USB cable is used for communication, a USB converter software
must be installed in addition (→ page 37).
2. The fraction collector is connected to the Chromeleon computer.
3. The fraction collector is set up in Chromeleon, as described in section 3.5
(→ page 39).
Before you can operate the fraction collector with Chromeleon, you have to connect the
timebase in which the fraction collector is installed to the Chromeleon client program
(→ section 5.2.1).
Two modes of software control are available:
• Direct control with the parameters and commands in the Commands dialog box (F8 box)
or on a control panel (→ page 46)
• Automated control with a control program (PGM) (→ page 52)
Operating Instructions Page 45
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5.2.1 Connecting to Chromeleon
1. Start the Chromeleon Server Monitor if it is not already running (→ page 39).
2. Click Start to start the server.
3. Start the Chromeleon client by clicking the Chromeleon icon on the desktop.
If the Chromeleon icon is not on the desktop, click Start on the taskbar, point to
Programs (or All Programs, depending on the operating system), point to Chromeleon,
and then click Chromeleon.
4. Connect the Chromeleon client to the timebase in which the fraction collector is
installed. For details about how to do this from the Commands dialog box, see
page 46. For details about how to do this on a control panel, see page 47.
Before turning off the fraction collector by the main power switch, always disconnect the
module in Chromeleon (Disconnect command).
5.2.2 Direct Control
With direct control, you select operating parameters and commands in the Commands
(F8) dialog box. Direct commands are executed as soon as they are entered. For routine
operation, most parameters and commands are also available on a control panel.
To open the Commands dialog box
1. Open a control panel (any panel is possible). To open a control panel, open the
Chromeleon Browser and double-click a control panel in the Dionex
Templates/Panels folder.
2. Connect the control panel to the timebase in which the fraction collector is installed.
On the Control menu, select Connect to Timebase, and then select the timebase on
the Timebase tab. For information about the Timebase dialog, click Help.
Tip: The Control menu is visible only when a control panel is already open.
3. Press F8 or click Command on the Control menu.
4. To view the available parameters and commands for the fraction collector, click the
plus sign beside AFC.
The commands and parameters available in the dialog box may vary, depending on the
Chromeleon version and the display filter level (Normal, Advanced, or Expert).
Page 46 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5. Change the display filter level if necessary. Right-click in the commands list and select
the filter level on the menu.
Fig. 16: Commands dialog box
6. Verify that the fraction collector is connected to Chromeleon. If it is not connected,
execute the Connect command.
In addition to the fraction collector commands and parameters, the Commands dialog box
provides access to all commands and parameters available for all devices that are installed in
the selected timebase.
To open the AFC-3000 control panel
1. Open the Commands dialog box and connect the AFC-3000 to Chromeleon
(→ page 46).
2. On the View menu, click Default Panel Tabset or click the corresponding icon on the
toolbar , and then connect to the Chromeleon server.
Chromeleon creates centralized control panels, called panel tabsets, for all timebases
available on the Chromeleon server. A panel tabset provides control panels for the
individual instruments in a timbase and, in addition, one or more panels for performing
system-wide functions, for example, creating and running sequences. For more
information about panel tabsets, see the Chromeleon Help.
3. On the Panel Tabset for your timebase, click the AFC-3000 tab page (→ Fig. 17).
Operating Instructions Page 47
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Fig. 17: AFC-3000 Control Panel
The control panel provides access to the operating parameters and commands required for
routine operation of the fraction collector. Additional functions are available in the
Commands dialog box. To open the Commands box from the panel tabset, select
Command on the Control menu.
Page 48 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5.2.3 Setting the Rack Configuration
Define the installed rack types in Chromeleon. It is not recommended to define a rack type
in a program file.
Important: The rack configuration must not be changed during fraction
collection, or during the course of a program file or sequence.
Important: Ne changez pas la configuration du rack au cours de la collection de
fraction, ou au cours d'un programme ou sequence.
1. Open the Commands dialog box (→ page 46).
2. Click the plus sign beside AFC.
3. Click Rack. In the Rack1 to Rack6 boxes, select the installed racks. If you have less
than six racks installed, select NotUsed for all unused rack positions. Note the
following restrictions:
• All racks have to be installed and defined following the order from Rack1 to Rack6.
• Unused rack positions (= NotUsed) must always be the last positions. For example,
it is not allowed to set NotUsed for Rack1 and then select a rack type for Rack2.
Observe the rack layout scheme (example for four 96 well plates see Fig. 18; details
for all rack types see page 31).
• Racks for 96 well plates must not be mixed with any other rack types.
4. Click Execute.
Fig. 18: Rack layout scheme (here: four 96 well plates)
(Tube numbering assumes SawVertical collect mode)
Operating Instructions Page 49
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5.2.4 Defining the Collect Mode
Define how the drop former travels across the racks.
Important: The CollectMode must not be changed during fraction collection, or
during the course of a program file or sequence.
Important: Ne changez pas le CollectMode au cours de la collection de fraction,
ou au cours d'un programme ou séquence.
1. Open the Commands dialog box (→ page 46).
2. Click the plus sign beside AFC.
3. Click CollectMode. In the CollectMode box, select the collect mode:
Vertical: The drop former follows a meander-shaped path. Be aware that this mode
minimizes tube change times, but negates imprinted tube numberings.
SawVertical (default): The drop former follows a saw-shaped path. The movement
matches the imprinted numbering on all rack types that are available from Thermo Fisher
Scientific.
Vertical SawVertical
Fig. 19: Vertical and SawVertical collect modes
Important: To avoid accidental contamination of empty tubes, never use
SawVertical collect mode in combination with Continue movement
mode.
Important: Afin d'éviter contamination des tubes, n'utilisez pas le mode de
collection SawVertical avec le mode de mouvement Continue.
4. Click Execute.
Tip: Schott bottles will always be processed in SawVertical collect mode. The
collect mode setting is not supported with Schott bottles.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5.2.5 Defining the Movement Mode
Define the diverter valve behavior during any drop former movements:
1. Open the Commands dialog box (→ page 46).
2. Click the plus sign beside AFC.
3. Click MovementMode. In the MovementMode box, select the movement mode:
Interrupt (default): If the diverter valve is open ("Collect") prior to any movement of the
drop former, the diverter valve will always close ("Drain") prior to a movement and will
open again ("Collect") after the target position has been reached. While the valve is
switched to drain and the drop former moves to the next tube, the delivered sample will
be directed to the waste.
Continue: The diverter valve maintains the previously defined valve state during and
after any diverter valve movement. In this mode, either all sample goes to the waste or all
sample goes through the drop former. This mode is designed to be used with the funnel
rack.
Important: To avoid accidental contamination of empty tubes, never use
Important: Afin d'éviter contamination des tubes, n'utilisez pas le mode de
4. Click Execute.
Continue movement mode in combination with SawVertical collect
mode.
mouvement Continue avec le mode de collection SawVertical.
Operating Instructions Page 51
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5.2.6 Automated Fraction Collection with Chromeleon
The Fraction Collection driver in Chromeleon facilitates the process of collecting fractions.
Before you start, verify that this driver is installed in the same timebase as the AFC-3000
(→ page 40).
With automated control, you create a program file (PGM) for automated operation of the
fraction collector. The Program Wizard assists you in creating a program for fraction
collection control.
On the File menu, select New, and then select Program File. The wizard guides you through
program creation. On each wizard page, make the desired settings or accept the default values.
For additional information about a page, click Help.
Fraction Collection - General Options
Determine whether fractions are collected by peak or by time.
• If you select Collect by peak using channel, choose the detection channel from the list.
Select the Collect outside peaks, too check box to collect sample in-between peaks.
• If you select Collect by time, type a time period in the Collection period box.
• Under Collect Time Frame, you can restrict the time for collecting fractions. Click
Collect only from and type or select the start and end times in the boxes.
Fig. 20: Fraction Collection Wizard: General Options page
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Fraction Collection - Collection Device Options
Enter collection parameters, such as the maximum tube volume, maximum number of tubes
per fraction, or total number of tubes.
• Type the volume of the installed tubes in the Max. tube volume box.
• Type the maximum of number of tubes to be used for collecting a single fraction in the
Max. number of tubes per fraction box.
• Type the total number of installed tubes in the Total number of tubes box.
Fig. 21: Fraction Collection Wizard: Collection Device Options page
Operating Instructions Page 53
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UltiMate 3000 Series:
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Fraction Collection - Peak Detection Options
Modify the peak detection algorithm for fraction collection if required (→ Fig. 22). For
more information, see the Chromeleon Help.
Fig. 22: Fraction Collection Wizard: Peak Detection Options page
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Fraction Collection - Delay and Detector Offset Options
Set the delay time or delay volume between the first detector in the system and the fraction
collector.
• Delay Time: This is a constant value which should only be applied with a constant pump
flow rate of your method. Note that changing the flow rate requires an according adaption
of the delay time to allow for proper fraction collection.
Delay Volume: This is the fluidic volume travelled by a peak after triggering the set start
threshold conditions between a detector and the separation point of the diverter valve.
Using the delay volume offers more flexibility, as Chromeleon automatically calculates the
delay time from the pump flow rate and the defined delay volume.
Both the delay volume and the delay time should be determined experimentally with a
suitable method. As a first approximation, the delay volume can be determined as a result
of the volume calculation of all tubing between the detector cell and the diverter valve.
Note that the volume of the drop former itself does not contribute to the delay volume but
to the carry over volume. It is recommended to perform a suitable test with some collected
fractions to assure proper functionality and fraction collection quality.
• If more than one detector is used, enter the delay time offset between the first detector after
the separation column and the subsequent detector or detectors under Detector Delay
Offsets.
Fig. 23: Fraction Collection Wizard: Delay and Detector Offset Options page
After completion of the wizard, Chromeleon creates a program file (method). Use this
program as a basis and add the required AFC-3000 commands and properties, for example,
by copying them from the example program provided in the appendix.
Operating Instructions Page 55
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
To modify a program
1. Open an existing program.
Select and double-click the program you want to open.
—or—
On the File menu, select Open. In the dialog box, select Program on the Object of Type
list and select the program.
Fig. 24: Chromeleon program file (shown in the Commands view, here not connected to a timebase)
2. Change the settings in the program as desired and add the required AFC-3000
commands. A program example can be found in the appendix.
Click Commands to open the Commands View. The Commands View shows the entire
program, listing the control commands in chronological order. Edit the parameter of
interest or enter a new parameter.
Important: The rack configuration (Rack) and the collect mode (CollectMode)
must not be changed during the course of a program file.
Important: Ne changez pas la configuration du rack (Rack) et le mode de
collection (CollectMode) au cours d'un programme.
3. To start the program, follow the steps below.
For more information on fraction collection, see the Chromeleon Help.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
To start a program
Program for sample analysis
1. Create a sample list (sequence). A sequence must include the program and a method
for evaluating the sample data (for example, for peak identification, area
determination, and amount determination).
2. Assign the program and method to each sample on the list.
3. Add the sequence to the batch and start the batch.
For information about each of the above steps, see the Chromeleon Help.
5.3 Shutting Down the Fraction Collector
To shut down the fraction collector, complete the following steps:
1. Flush flow paths as required and as outlined in section 5.4.
2. Shut down the HPLC system to which the fraction collector is connected as is required
and as instructed via the associated documentation and outlined procedures therein.
3. Turn off the AFC-3000 power switch.
5.4 Flushing the Rinse Station and Eluent Flow Path
Generally, you can operate the AFC-3000 without flushing the rinse station. Under normal
circumstances, you can simply drain the rinse station prior to shutting down the fraction
collector. However, you should flush the rinse station and flow path under two
circumstances:
• during initial startup of the AFC-3000, after installation
• following the use of strong bases, acids, or organic solvents as may be present in the eluent
Flushing the rinse station during initial startup of the AFC-3000 removes any
contaminants. Flushing the rinse station after using strong rinse/eluent agents/solvents
prevents degradation and failure of the flow path components. If additives such as buffers
are used as a part of the mobile phase, flush the system with several volumes of a suitable
solvent before shutting the AFC-3000 down. This will prevent buildup of precipitates
inside the flow path.
To flush the rinse station and flow path, flush the entire HPLC system as outlined in the
system's operating instructions.
Operating Instructions Page 57
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
5.5 Routine and Preventive Maintenance
Routine maintenance of the AFC-3000 consists of regular cleaning of specific fraction
collector components. Routine maintenance also includes checking AFC-3000 components
for leaks or other damage. Additional periodic maintenance tasks may be required,
including replacement of the following fraction collector components: sample inlet tubing,
rinse station tubing, and fraction tray.
Perform the maintenance procedures listed in the following table at regular intervals to ensure
optimum performance and maximum uptime of the fraction collector. The exact maintenance
schedule for the fraction collector will depend on numerous factors, and will vary by
application.
Frequency What you should do ...
Daily
Check the fluid lines for any signs of leakage (→ page 63).
Check the fluid connections for any signs of salt deposits.
Clean the fraction collector (general) (→ page 62).
When using buffer solutions, flush the fraction collector flow path
thoroughly after use with a suitable solvent.
Weekly
Periodically
Clean the fraction collector (thorough) (→ page 63).
Inspect the tubing for possible damage, such as cracks, nicks, cuts,
or blockages.
Replace rinse station tubing and sample inlet/waste tubing
(connected to the diverter valve) as needed.
Check the rinse station tubing connected to the rinse station. Verify
that the tubings are not clogged and are routed to the appropriate
drain system or waste container without trapping liquid in the
tubing, which could cause an overflow condition. Check the
volume of the liquid in the waste container and empty as needed.
Check the following components for wear and replace if necessary:
• Drop former
• Diverter valve
• General tubing
If you fail to replace these components when they deteriorate, the
fraction collector will not function properly.
Annually
Have Thermo Fisher Scientific Service check the fraction collector
once a year to prevent, for example, contamination and excessive
wear.
Page 58 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
6 Troubleshooting
6.1 General Notes
The AFC-3000 is both easy to operate and reliable. When the fraction collector does not
function properly, isolate the problem to determine if it originates in the Chromeleon
computer, the analytical instrument, the communications cable, or the fraction collector
itself. The following sections help you to identify and eliminate the source of problems that
may occur during the operation of the AFC-3000.
If you determine the problem is in the AFC-3000, check the power system, the
communications interface, or the diverter valve assembly to locate the cause of the problem
and resolve it.
If a problem occurs, always check the Chromeleon audit trail for messages (→ section 6.3).
If you cannot solve a problem using the steps given in this chapter, please contact Thermo
Fisher Scientific Service for Dionex HPLC Products.
6.2 Power and Communication Problems
The following table provides information about operating problems and lists probable
causes, as well as remedial actions.
Problem Probable Cause Remedial Action
The blue LED
power indicator is
off.
The AFC-3000 does
not work correctly
when controlled by
Chromeleon.
The instrument is not connected
to the mains.
The power is turned off. Turn on the fraction collector power.
The power switch of the external
power supply is turned off.
An error occurred in the
electronic system.
There is no connection between
the fraction collector and the
Chromeleon computer.
Connect the power cord
(→ section 3.4.1; page 35).
Turn on the power switch of the
external power supply (if equipped).
Contact Service.
Check the USB or RS-232 cable and
connection to the computer. If the cable
is connected, ensure that it is tightened
properly.
Verify that the cable is connected to
COM 1 port on the AFC-3000 rear
panel, or to the appropriate module
USB hub if connecting in that manner
(→ section 3.4; page 35).
Test the interface (→ section 4.3;
page 43).
Operating Instructions Page 59
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Problem Probable Cause Remedial Action
The AFC-3000 does
not work correctly
when controlled by
Chromeleon.
(Cont'd) The USB port on the computer is
The fraction collector is not
correctly configured in
Chromeleon.
not ready for operation.
Check the configuration for the correct
COM port selection
(→ section 3.5.2; page 40).
Check the USB port on the computer.
6.3 Messages in the Chromeleon Audit Trail
When the fraction collector is operated with Chromeleon, messages are displayed in the
Chromeleon Audit Trail.
The following table lists fraction collector-related messages along with appropriate remedial
actions. In addition to the messages in the table, other messages may appear. In this case, note
the exact wording of the message and contact Thermo Fisher Scientific Service for Dionex
HPLC Products if you are unable to eliminate the problem.
Message Remedial action
Can't open communication channel
Serial Device Driver\COM2.
Check the configuration for the correct COM port selection
(→ section 3.5.2; page 40).
Command for function Connect
failed.
Command for function Rack
failed.
Command to change property
TubePosition failed.
Device is not remote. The AFC-3000 is not connected to Chromeleon. Execute the
Error P0040: Value out of range. Verify that you have entered values that are within the allowed
Internal error: A required
parameter was missing.
Invalid enumerated value 3.
Parameter TubePosition value
exceeded its allowed range. Set to
closest valid value 384.
The AFC-3000 cannot connect to Chromeleon. See
Communication Problems (→ page 59).
Verify that you have configured the racks in the appropriate
order, and that you have not configured more racks than can be
installed (→ page 49).
You have entered a tube position that exceeds the number of
positions on the installed racks. Verify that you have defined a
valid rack type. Enter a valid tube position.
Connect command.
range.
Verify that you have defined a legal rack type for all used rack
positions, and NotUsed for unused rack positions (→ page 49).
Verify that you have entered valid rack types only (→ page 49).
You have entered a tube position that exceeds the number of 384.
Enter a valid tube position. This error appears only with the use
of the 96Pos rack type.
Page 60 Operating Instructions
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
7 Service
This chapter explains how to clean the AFC-3000, inspect it for leaks, and replace
damaged components.
Important: Discharge static buildup and ground to the fraction collector base or
cabinet before performing any maintenance. Do not touch or
short-circuit bare contacts (for example, COM1 or auxiliary ports).
Important: Déchargez accumulation d'électricité statique et mettez à la terre la
base et le cabinet du collecteur de fractions avant de procéder à tous
travaux d’entretien. Ne touchez ou court-circuitez pas des contacts
nus (par exemple, COM1 ou ports auxiliaires).
7.1 General Notes and Safety Precautions
The following sections describe all procedures for the fraction collector that the user may
perform. All other maintenance and service procedures must be performed only by Thermo
Fisher Scientific service personnel.
Warning: The fluid components of the device may be filled with solvents that
are harmful to health. In the same way, harmful substances may be
present at the wetted parts. Wear appropriate personal protective
equipment. Rinse the fluid components with an appropriate solvent
to remove harmful substances.
For information about the proper handling of a particular substance
and for advice on specific hazards, refer to the material safety data
sheet for the substance you are using. Observe the guidelines of
Good Laboratory Practice (GLP).
Avertissement: Les composants fluidiques de l'instrument peuvent être remplis de
solvants nocifs. De la même, des substances nocives peuvent être
présents sur les pièces en contact avec le fluide. Portez l'équipement
de protection personnel approprié. Rincez les composants fluidiques
avec un solvant approprié afin d'éliminer les substances nocives.
Pour les informations sur la manipulation correcte des substances et
des recommandations pour les situations de risque spécifiques,
veuillez consulter la fiche de données de sécurité des substances que
vous utilisez. Veuillez respecter des directives des Bonnes Pratiques
de Laboratoire (BPL).
Operating Instructions Page 61
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Before starting maintenance or service procedures, observe the following precautions:
• For all service and repair procedures, observe all precautionary statements provided in
these operating instructions.
• When performing maintenance or repair work on the fluidic components, always move the
diverter valve assembly into the appropriate position to facilitate safe working conditions
and to allow access to all components without obstructions. The power should be turned
off for almost all service procedures to ensure the safety of the operator/technician.
(Follow the instructions in the sections for the individual components.)
• Use only the original spare parts authorized for the device by Thermo Fisher Scientific.
• Before returning any instrument to for repair, contact Thermo Fisher Scientific Service for
Dionex HPLC Products. An RMA (Return Material Authorization) number is required to
track your instrument. Always use the original shipping container and observe the packing
instructions when shipping the fraction collector. Shipping the instrument in anything other
than the original packaging voids the warranty. If the appropriate shipping container is not
available, appropriate shipping containers and packing material can be ordered from the
Thermo Fisher Scientific sales organization for for Dionex HPLC Products. The packing
instructions are included in the “Installation and Qualification Documents for
Chromatography Instruments” binder and are available on request. For more information,
see the warranty statement in the terms of sale.
For instructions on shutting down the fraction collector, see section 5.3 (→ page 57).
7.2 Cleaning the Fraction Collector
Cleaning the AFC-3000 is the primary maintenance task you perform. Failure to do so
regularly causes increased wear and reduces the fraction collector’s life.
You must clean the AFC-3000 both daily and weekly to prevent damage and extend its life. It
is especially important to clean up spills and remove contaminants, such as abrasives, from
the fraction collector’s moving parts. It may also be necessary to chemically neutralize spills.
The following sections explain daily and weekly cleaning procedures.
7.2.1 Daily External Cleaning
Use of the AFC-3000 often results in spills on fraction collector components such as the
fraction tray. Good maintenance requires that you clean the fraction collector daily. To do
so, complete the following steps:
1. Shut down and unplug the fraction collector.
Tip: For information about shutting down the fraction collector, see section 5.3
(→ page 57).
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
2. Wipe the fraction tray, fraction collector cabinet, and fraction collector arm using a
towel dampened with a lab-grade cleaning agent.
Important: Do not allow the cleaning agent to come into contact with the lead
screws. Never lubricate either of the two lead screws.
Important: Assurez-vous que les broches filetées ne soient jamais en contact
avec les agents de nettoyage. Ne jamais lubrifiez les deux broches
filetées.
3. Repeat step 2, using a towel dampened with clear water. This process removes any
remaining contaminants.
4. Dry the fraction tray, fraction collector cabinet, and fraction collector arm using a dry
towel. The AFC-3000 must be thoroughly dry before you turn the fraction collector
power on.
7.2.2 Weekly Cleaning
Although cleaning the fraction collector daily removes spills and contaminants from most
of the fraction collector components, it is necessary to clean the AFC-3000 more
thoroughly once a week. To do so, complete the following steps:
1. Shut down and unplug the fraction collector.
2. Remove the rack.
3. Wipe the fraction collector exterior, base and tray until they are clean, using a towel
dampened with a lab-grade cleaning agent, followed by a towel dampened with clear
water.
4. Replace the rack on the fraction collector tray.
7.3 Checking for Leaks
Several of the fraction collector components have a limited life and will wear out under
normal use; the drop former, eluent tubing and the rinse station tubing. Standard
maintenance procedures require that you periodically check these components for leaks. To
do so, complete the following steps:
1. Shut down and unplug the fraction collector.
2. Visually inspect the drop former, eluent tubing and rinse station and tubing for leaks
or signs of deterioration or salt loading.
If you detect a leak or other damage to a fraction collector component, you must replace it.
Operating Instructions Page 63
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
7.4 Installing the Kit for Normal-Phase LC
Description Part No.
Kit for normal-phase LC, including:
6702.0400
1.0 mm ID stainless steel drop former and grounding cable
Warning: Do not perform normal-phase applications without a proper
grounding protection of the drop former. Non-conductive, non-polar
normal-phase eluents, for example hexane, can lead to a build-up of
electrostatic charges on an ungrounded drop former. The electrostatic discharge can create a fire and/or explosion hazard.
Avertissement: N'effectuez pas d'applications en phase normale sans vous être assuré
que votre faiseur des gouttes est proprement isolée et mise à la terre.
Les solvants de la phase normale, non-conducteurs et non-polaires,
par exemple hexane, peuvent entraîner une accumulation de charges
électrostatiques sur le faiseur des gouttes en l’absence de mise à la
terre. Le décharge électrostatique peut représenter un risque
d’incendie et/ou d'explosion.
1. Switch the valve to Drain position, then shut down and unplug the fraction collector.
Set the pump flow to 0.
2. Carefully move the diverter valve assembly along the Y arm to the front.
3. Remove the current drop former. Then screw the SST drop former finger-tight into the
diverter valve.
4. Push the open clip end of the grounding cable from the normal-phase kit between the
diverter valve and the drop former (→ Fig. 25).
Fig. 25: Installing the normal-phase ground strip
Page 64 Operating Instructions
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UltiMate 3000 Series:
Ground cable
screw
AFC-3000 Automated Fraction Collector
5. Remove the ground cable screw and reinstall with the normal-phase grounding cable
attached. Make sure to lead cable along the front side of the diverter valve assembly
(→ Fig. 26).
Fig. 26: Normal-phase ground strip
Tip: The carry-over volume of the normal-phase kit is the same as for the standard
configuration.
Operating Instructions Page 65
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
7.5 Installing the Kit for Low Flow Rates
Description Part No.
Kit for low flow rates, including:
6702.0300
1.0 mm ID diverter valve with 0.4 mm ID PEEK drop former
The kit for low flow rates includes a tool for installation and a piece of 0.3 mm ID PTFE
tubing. Before you start, make sure that you have all parts of the kit at hand.
1. Shut down and unplug the fraction collector. Set the pump flow to 0.
2. Dismount the current diverter valve from the assembly (→ page 67).
3. Connect the diverter valve cable to the auxiliary I/O connection on the AFC-3000 rear
panel (→ Fig. 2, page 18). Tighten the knurled nut finger-tight.
4. Remove the two valve screws that are attached to the new diverter valve.
5. Remove the drop former and drop former screw from their packaging and insert the
drop former into the screw (→ Fig. 27). Then place the screw in the diverter valve
(bottom side). Use the screwdriver tool shipped with the kit to carefully tighten the
screw. You may have to retighten the screw if you observe leakage during operation.
Fig. 27: Installing the low-flow drop former
6. Mount the diverter valve to the diverter valve assembly by using the hexagon wrench
shipped with the kit (→ Fig. 28, page 67). The diverter valve must be mounted with
the waste connection facing upward.
7. Tighten the finger-tight knurled screw that attaches the rear cable to the mounting
block (→ Fig. 30, page 68).
8. Tighten the finger-tight knurled screw that attaches the rear cable to the diverter valve
and connect the grounding cable from the rear cable to the ground cable screw and
tighten the screw (→ Fig. 29, page 68).
Page 66 Operating Instructions
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UltiMate 3000 Series:
Diverter valve
screws
Hexagon
wrench
AFC-3000 Automated Fraction Collector
9. Install the diverter valve connections (→ section 3.3.2, page 28). A low-flow inlet
capillary is shipped with the kit.
10. Verify that the alignment is still correct (→ page 44).
Tips: After installing the low-flow inlet capillary, always determine the delay time or
volume. Note that the carry-over volume of the kit for low flow rates is smaller
than with the standard configuration.
Exclusively use the low-flow drop former within the specified flow rates (up to
10 mL/min). At higher flow rates, the jet from the drop former may cause
sprays or spillages.
7.6 Replacing the Diverter Valve
Description Part No.
Diverter valve with 1.0 mm ID PEEK drop former
6702.0500
(up to 150 mL/min)
Diverter valve with 0.4 mm ID drop former (up to10 mL/min),
6702.0300
including tools and tubing
The rear cable is pre-installed on the diverter valve.
1. Shut down and unplug the fraction collector. Set the pump flow to 0.
2. Carefully move the diverter valve assembly along the Y arm to the front.
3. Remove the sample inlet and waste tubing and unscrew the current drop former from
the diverter valve.
4. Loosen the two diverter valve screws by using the hexagon wrench shipped with the
kit (→ Fig. 28).
Fig. 28: Dismounting the diverter valve
Operating Instructions Page 67
Page 74
UltiMate 3000 Series:
Ground cable
screw
Rear cable screw
AFC-3000 Automated Fraction Collector
5. Loosen the two finger-tight knurled screws that attach the rear cable and grounding
cable to the diverter valve assembly (→ Fig. 29).
Fig. 29: Cable screws on the diverter valve assembly
6. Disconnect the diverter valve cable from the auxiliary I/O connection on the
AFC-3000 rear panel (→ Fig. 2).
7. Loosen the finger-tight knurled screw that attaches the rear cable to the mounting
block on the AFC-3000 rear panel (→ Fig. 30).
Fig. 30: Removing the rear cable screw
8. Reinstall the new diverter valve in reverse order. If you are replacing a low-flow
diverter valve, also see section 7.5 (→ page 66).
Page 68 Operating Instructions
Page 75
UltiMate 3000 Series:
Hole
Screw
wrong
right
AFC-3000 Automated Fraction Collector
7.7 Replacing the Universal Drip Tray
For proper operation, the universal drip tray must be properly seated on the base plate of
the fraction collector. An improperly installed universal drip tray may lead to the
impression that the fraction collector is misaligned.
1. Remove the rack adapter or the fraction racks from the universal drip tray as shown in
Fig. 11 on page 33.
2. Lift the drip tray from the base plate such that the holes in the two screws in the base
plate disengage from the two holes in the drip tray.
3. Place the new universal drip tray on the fraction collector such that the two screws on
the base plate are placed inside the holes in the tray.
Fig. 31: Installing the universal drip tray
4. Verify that the universal drip tray is not misaligned. This means that the drip tray and
the base plate have to be arranged in parallel (compare figures below).
Fig. 32: Misaligned universal drip tray Fig. 33: Properly aligned universal drip tray
7.8 Updating the Firmware
The fraction collector is shipped with the most recent firmware version. However, in some
cases, a firmware update may be required.
Only qualified service personnel should perform firmware updates. For more information,
please contact Thermo Fisher Scientific Service for Dionex HPLC Products.
Operating Instructions Page 69
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Page 70 Operating Instructions
Page 77
8 Technical Information
Tube capacity:
Depends on rack configuration;
standard configuration: 180 x 8 mL tubes (13
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
mm x 100 mm)
Tube change time:
Maximum tube height:
Maximum flow rate:
Carry-over volume:
Wetted parts:
Control:
GLP features:
Power requirements:
Emission sound pressure
level:
Environmental
conditions:
Typically 0.2 to 0.4 s, depending on collect mode and rack type
150 mm
150 mL/min
15 µL (standard), 1.3 µL (with optional low-flow kit)
ETFE, FFKM (Simriz®), PFA, PEEK (with standard and low-flow drop
former), stainless steel (with grounded drop former for normal-phase LC)
All functions software-controlled via RS-232 or USB; requires Chromeleon
Chromatography Management System
In Chromeleon:
All system parameters are recorded in the Chromeleon audit trail.
100–240 V, 47-63 Hz AC 1.9 A with an output of DC 24V, maximum 3.33 A
Automatic voltage selection
< 70 dB(A), typically 62 dB(A)
Range of use: Indoor use
Temperature: 10 to 35 °C (50 to 95 °F)
Air humidity: 20-70% relative humidity, non-condensing
Overvoltage category: II
Pollution degree: 2
Dimensions (h × w × d):
Weight:
43 x 34 x 46 cm (16.5 x 13.4 x 18.1 in.)
8 kg (17.7 lbs)
Technical information: August 2013
All technical specifications are subject to change without notice.
Operating Instructions Page 71
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Page 72 Operating Instructions
Page 79
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
9 Accessories, Spare Parts, and Consumables
Accessories, spare parts, and consumables for the fraction collector are always maintained
at the latest technical standard. Therefore, part numbers are subject to alteration. However,
updated parts will always be compatible with the parts they replace.
Because the life-span of the fraction tubes varies, you should maintain an adequate supply of
spare tubes. When you need to purchase additional supplies, it is extremely important that you
choose the appropriate sizes and materials. When you purchase fraction tubes, make sure they
meet the following requirements:
• The diameter of the fraction tube matches the rack size you are using.
• The height does not exceed 150 mm.
• The material is compatible with the samples and solvents you are using.
Important: Do not use mismatching fraction tubes. Be sure your tubes meet the
given requirements.
Important: N'utilisez pas de tubes de fraction incompatible. Assurez-vous que
vos tubes répondent aux exigences données.
9.1 Standard Accessories (included in the shipment)
The following accessories are shipped with the fraction collector. (The list is subject to
change without notice). The part number always refers to the packing unit. For more
information, contact the Thermo Fisher Scientific sales organization for Dionex HPLC
Products.
Description Part No. Quantity in the
accessories kit
AFC-3000 accessories kit, including:
24 V external power supply 6702.9010 1
Diverter valve assembly, including screws for installation
and 1 mm ID PEEK drop former
Fluidics kit, incl. sample inlet tubing (1.0 mm x 4 m) and
waste tubing (1.0 mm x 1.8 m) with nuts and ferrules
Drain tubing (2 x 1.8 m) Included in 6040.0005 2
Elbow, Y and T pieces for drain connections Included in 6040.0005 7
- 1
- 1
Rack (90 position, 8 mL; 13 mm x 100 mm) 6702.0090 2
Polypropylene tubes (8 mL, OD 13 mm) - 180
USB cable, type A to type B, USB 2.0, 5 m 6911.0002 1
RS-232 serial cable, 9pin–9pin, fem–fem, 5m 8914.0153 1
Capillary marker Included in 6040.2399 5
Spiral tube - 1
Operating Instructions Page 73
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
9.2 Optional Accessories
Optional Accessory Part No. Remarks
Kit for low flow rates 6702.0300 Kit for flow rates up to 10 mL/min.
Includes different diverter valve
assembly, drop former, and tubing.
Kit for normal-phase LC 6702.0400 SST drop former for use with normal-
phase solvents. Provides grounding via a
cable.
Drain kit for UltiMate 3000 systems
Fraction Collection License 5960.0038 Additional Chromeleon license required
Extended Fraction Collection License 5960.0039 Additional Chromeleon license required
Rack (21 position, 50 mL; 30 mm x 100 mm ) 6702.0021
Rack (24 position, 30 mL; 24 mm x 100 mm) 6702.0024
Rack (40 position, 20 mL; 20 mm x 100 mm) 6702.0040
Rack (60 position, 14 mL; 16 mm x 100 mm) 6702.0060
Rack (10 position, WPS, 10 mL; 22 mm OD) 6820.4086
Rack (22 position WPS, 4 mL; 15 mm OD) 6820.4084
Rack (40 position, WPS, 2 mL; 12 mm OD) 6820.4070
Adapter for WPS racks 6702.0100 Required for use of all WPS racks with
Rack (36 position, Foxy; scintillation vials,
28 mm OD)
Rack (60 position, Foxy; 1.5 mL) 5701.2023
Rack (72 position, Foxy; mini tubes, 18 mm
OD)
6040.0005 The kit includes all required components
for system drainage.
for basic fraction collection (→ page 21).
for advanced fraction collection
(→ page 21). This license includes the
Fraction Collection license.
Racks can be used for WPS-3000 and
ACC-3000 autosamplers.
the AFC-3000.
5701.2025
Racks can be used for Foxy Jr./R1
fraction collectors.
5701.2024
Positioning pins for Foxy racks 6702.9006 Pins facilitate installation of Foxy racks
on the AFC-3000.
Adapter for 4 well plates 6702.0200 An adapter is required for using well
Adapter for 2 well plates (Foxy Jr./R1) 5701.2021
Bottle (0.25 L) 2270.0026
Funnel rack 6702.1021 To be used in conjunction with the 21
plates with the AFC-3000.
position rack (6702.0021). Tubing
included.
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
9.3 Consumables and Spare Parts
The part number always refers to the packing unit. Unless otherwise stated, the packing unit is
1 unit. For more information, contact the Thermo Fisher Scientific sales organization for
Dionex HPLC Products.
Description Part No.
24 V external power supply 6702.9010
Bottle (0.25 L) 2270.0026
Capillary markers (10 pcs) 6040.2399
Diverter valve, 0.4 mm ID drop former, and tubing ("Kit for low flow rates") 6702.0300
Diverter valve (replacement for standard diverter valve) 6702.0500
Drop former, 0.4 mm ID, PEEK, including drop former replacement tool 6702.0305
Drop former, 1 mm ID, PEEK (replacement for standard drop former) 6702.0505
Drop former, 1 mm ID SST, including grounding cable ("Kit for normal-phase LC") 6702.0400
Funnel rack 6702.1021
Rack (10 position, WPS, 10 mL; 22 mm OD) 6820.4086
Rack (21 position, 50 mL; 30 mm x 100 mm ) 6702.0021
Rack (22 position WPS, 4 mL; 15 mm OD) 6820.4084
Rack (24 position, 30 mL; 24 mm x 100 mm) 6702.0024
Rack (36 position, Foxy; scintillation vials, 28 mm OD) 5701.2025
Rack (40 position, 20 mL; 20 mm x 100 mm) 6702.0040
Rack (40 position, WPS, 2 mL; 12 mm OD) 6820.4070
Rack (60 position, 14 mL; 16 mm x 100 mm) 6702.0060
Rack (60 position, Foxy; 1.5 mL) 5701.2023
Rack (72 position, Foxy; mini tubes, 18 mm OD) 5701.2024
Rack (90 position, 8 mL; 13 mm x 100 mm) 6702.0090
Adapter for 4 WPS racks 6702.0100
Adapter for 4 well plates 6702.0200
Adapter for 2 well plates (Foxy Jr./R1) 5701.2021
RS-232 serial cable, 9pin–9pin, fem–fem, 5m 8914.0153
Universal drip tray 6702.9025
USB cable, type A to type B, USB 2.0, 1 m 6911.0002
Operating Instructions Page 75
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
10 Appendix - Chromeleon Example Program
;Example program for fraction collection with the AFC-3000
Automated Fraction Collector and the following instruments:
HPG-3x00, WPS-3000SL, VWD-3x00
TempCtrl = On
Temperature.Nominal = 20.0 [°C]
Temperature.LowerLimit = 4.0 [°C]
Temperature.UpperLimit = 45.0 [°C]
ReadyTempDelta = None
Pressure.LowerLimit = 0 [bar]
Pressure.UpperLimit = 150 [bar]
MaximumFlowRampDown = 30.000 [ml/min²]
MaximumFlowRampUp = 30.000 [ml/min²]
%A.Equate = "%A"
%B.Equate = "%B"
DrawSpeed = 20.000 [µl/s]
DrawDelay = 3000 [ms]
DispSpeed = 2.000 [µl/s]
DispenseDelay = 2000 [ms]
WasteSpeed = 4.000 [µl/s]
SampleHeight = 1.000 [mm]
InjectWash = NoWash
LoopWashFactor = 0.000
PunctureOffset = 0.0 [mm]
Sampler.PumpDevice = "Pump"
InjectMode = Normal
SyncWithPump = Off
Pump_Pressure.Step = Auto
Pump_Pressure.Average = On
Data_Collection_Rate = 25.0 [Hz]
TimeConstant = 0.06 [s]
AFC.Valve = Drain
;Close the diverter valve prior to a run to avoid
accidental spillages
AFC.CollectMode = SawVertical
;Define how the drop former travels over the collection
vessels.
;Do only define once and do not change during a run or
a sequence
AFC.MovementMode = Interrupt
;Define how the diverter valve behaves between tube
changes.
AFC.TubePosition =
FractionCollection.TubePosition
;Synchronize the fraction collector position with
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
;the current position of the fraction collection driver
prior to a run
;*****************************************************************
;* Definition of triggers for fraction collection starts here.
;*****************************************************************
Trigger FracStart
FracStartDetected
AFC.Valve = Collect
Log AFC.TubePosition
Log
FractionCollection.TubePosition
EndTrigger
Trigger TubeChange
FracTubeChange
AFC.Valve = Drain
AFC.TubePosition =
FractionCollection.TubePosition
AFC.Valve = Collect
Log AFC.TubePosition
Log
FractionCollection.TubePosition
EndTrigger
Trigger FracEnd
FracEndDetected
AFC.Valve = Drain
AFC.TubePosition =
FractionCollection.TubePosition
Log AFC.TubePosition
Log
FractionCollection.TubePosition
EndTrigger
;*****************************************************************
;* Definition of triggers for fraction collection ends here.
;*****************************************************************
;*****************************************************************
;* Definition of fraction collection driver settings starts here.
;*****************************************************************
FractionCollection.PumpDevice = "Pump"
TubeMaxVolume = 40.000000 [ml]
TotalNumberInstalled = 42
MaxTubesPerFraction = 10
TubeWrapping = No
TubeChangeDuration = 0.5 [s]
DetectionChannel1.Name = "UV_VIS_1"
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
PeakStartThreshold = 10.00 [signal]
PeakStartSlope = 0.500 [signal/s]
PeakStartTrueTime = 1.00 [s]
PeakEndThreshold = 9.00 [signal]
PeakEndSlope = -1.000 [signal/s]
PeakEndTrueTime = 1.00 [s]
DerivStep = 1.00 [s]
ThresholdNoPeakEnd = 2000.00 [signal]
ThresholdDoNotResolve = Off
PeakMaxSlope = Off
PeakMaxTrueTime = 1.00 [s]
PeakStartCurve = 1.000 [signal/s²]
PeakEndCurve = 1.000 [signal/s²]
BaselineDrift = 0.000 [signal/s]
BaselineOffset = 0.000 [signal]
DelayTime = 5.5 [s] ;To be
determined by suitable experiment on site.
OffsetTime = 0.0 [s]
;*****************************************************************
;* Definition of fraction collection driver settings ends here.
;*****************************************************************
UV_VIS_1.Wavelength = 190 [nm]
Flow = 25.000 [ml/min]
%B = 0.0 [%];isocratic
Pump.Curve = 5
;*****************************************************************
;* Sample run starts here.
;*****************************************************************
0.000 Autozero
Wait UV.Ready and
Sampler.Ready
Inject
Pump_Pressure.AcqOn
CollectFractions = By_Peak
CollectOutsidePeaks = No
UV_VIS_1.AcqOn
10.000 Pump_Pressure.AcqOff
CollectFractions = No
UV_VIS_1.AcqOff
10.142 AFC.Valve = Drain
;Close the diverter valve after a run to avoid
accidental spillages
End ;End time is crooked because of internal processing
times required by triggers
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
;*****************************************************************
;* Sample run ends here.
;*****************************************************************
Page 80 Operating Instructions
Page 87
11 Index
A
Accessories
Optional ........................................................ 74
Standard ........................................................ 73
Alignment .......................................................... 44
Auxiliary I/O Connector .................................... 19
C
Chromeleon.................................................. 21, 45
Automated Control ........................................ 52
Batch ............................................................. 57
CollectMode .................................................. 50
Connect ......................................................... 46
Direct Control ............................................... 46
Example Program .......................................... 77
Fraction Collector Configuration .................. 40
Fraction Collector Installation ....................... 39
Installation Program ...................................... 39
License .......................................................... 21
MovementMode ............................................ 51
Program Start ................................................ 57
Rack Configuration ....................................... 49
Server Configuration Program ...................... 39
Server Monitor .............................................. 39
Chromeleon Dialog Boxes
AFC ............................................................... 41
Dionex AFC-3000 Fraction Collector ........... 40
Cleaning ............................................................. 62
Daily .............................................................. 62
Weekly .......................................................... 63
Collect Mode ..................................................... 50
Commands (Dialog Box) ................................... 46
Configuration Page ............................................ 40
Configurations ................................................... 15
Connection ......................................................... 35
Chromeleon Computer .................................. 36
Diverter Valve ............................................... 28
Power ............................................................ 35
RS-232 Cable ................................................ 36
USB Cable .................................................... 36
Control (Automated) .................................... 45, 52
Control (Direct) ........................................... 45, 46
Control Panel ..................................................... 47
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
Diverter Valve Assembly .................................. 27
Adjust Height ................................................ 33
Alignment ..................................................... 44
Mount Assembly ........................................... 27
Diverter Valve Connector .................................. 19
Drain Tubing ..................................................... 29
Drip Tray Tubing ............................................... 29
Drop Former .......................................... 15, 33, 44
E
Example Program .............................................. 77
F
Facility Requirements ........................................ 23
Firmware Update ............................................... 69
Flush Rinse Station ............................................ 57
Found New Hardware Wizard ........................... 37
Foxy Racks ........................................................ 32
Fraction Collection Driver ........................... 39, 52
Fraction Collection Wizard ............................... 52
Fraction Tube Racks .............................. 20, 30, 49
Fraction Tubes ................................................... 20
Front Panel ........................................................ 17
I
Inspect Components .......................................... 43
Installation ......................................................... 26
in Chromeleon .............................................. 39
Power Connection ......................................... 35
Test ............................................................... 43
Verification ................................................... 43
Intended Use ...................................................... 10
Interface Test ..................................................... 43
K
Kit for Low Flow Rates ............................... 15, 66
Kit for Normal-Phase LC .................................. 15
L
Leaks ................................................................. 63
Low-Flow Kit .............................................. 15, 66
M
D
Daily Cleaning ................................................... 62
Diverter Valve ............................................. 28, 67
Connections................................................... 28
Replace .......................................................... 67
Maintenance ................................................ 45, 58
Maintenance Intervals ....................................... 58
Messages ........................................................... 60
Movement Mode ............................................... 51
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UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
N
Normal-Phase LC ........................................ 15, 64
O
Operating Instructions ......................................... 1
Operation ........................................................... 45
Chromeleon ................................................... 45
Safety Precautions ........................................... 3
Safety Symbols ............................................... 2
Operation Principle ............................................ 14
P
Panel Tabset ....................................................... 47
Power Connection .............................................. 35
Power Switch ..................................................... 19
Power-Up ........................................................... 45
Program Creation ............................................... 52
R
Racks ..................................................... 20, 30, 49
Rear Panel .......................................................... 18
Rear Panel Connector .................................. 19, 36
Repair ................................................................ 61
General Notes ............................................... 61
Replace Diverter Valve ................................. 67
Rinse Station ................................................ 29, 57
Rinse Station Tubing ......................................... 29
RMA Number .................................................... 62
RS-232 Port ....................................................... 19
S
Safety Information ............................................... 2
Safety Precautions ............................................... 3
Safety Symbols .................................................... 2
Serial Communication ....................................... 41
Serial Port .......................................................... 19
Server Configuration Program ........................... 39
Server Monitor ................................................... 39
Service ............................................................... 61
Check for Leaks ............................................ 63
Clean Fraction Collector ............................... 62
Firmware Update .......................................... 69
General Notes ............................................... 61
Install Low-Flow Kit .................................... 66
Install Normal-Phase Kit .............................. 64
Replace Diverter Valve ................................ 67
Safety Measurements .................................... 61
Shutdown ........................................................... 57
Software Control ......................................... 21, 45
Solvents ............................................................. 10
Spare Parts ......................................................... 75
Specification ...................................................... 71
Stainless Steel Drop Former ........................ 15, 64
Startup ............................................................... 45
T
Technical Information ....................................... 71
Tray ....................................................... 20, 30, 69
Troubleshooting ................................................ 59
Communication Problems ............................ 59
Messages....................................................... 60
No Power ...................................................... 59
Tubes ................................................................. 20
U
Unit Description ................................................ 13
Configurations .............................................. 15
Overview ...................................................... 13
Principle of Operation .................................. 14
Universal Drip Tray............................... 20, 30, 69
Unpacking ......................................................... 24
USB ............................................................. 19, 36
User Information ............................................... 11
V
Verify Installation.............................................. 43
W
Weekly Cleaning ............................................... 63
Page 82 Operating Instructions
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