Thermo Scientific A2-OK Operating And Maintenance Manual

Horizontal System

Model A2-OK

Operating and Maintenance Manual 7219011 Rev. 0

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Thermo Scientific

MANUAL NUMBER 7219011

0 -- 4/13/12 Transfer to Marietta (was 11/2002) ccs

REV ECR/ECN DATE DESCRIPTION By

Preface

Horizontal System i

Thermo Scientificii Horizontal System

Preface

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance.

s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Warning To avoid the risk of personal shock, always disconnect the gel box from the power supply. Further, the

power supply must be equipped with a shut-down-on-disconnect circuit. Do not move the unit unless the power
source to the unit has been disconnected.

s

Statement of Proper Use: Use this product only for its intended purpose as described in this manual. Do not use
this product if the power leads are damaged or if any of its surfaces are cracked.

Warning Running conditions for this unit should not exceed the name plate readings found on the lower buffer
chamber.

s

This Owl System is designed to meet IEC 1010-1 safety standards (IEC 1010-1 is an internationally accepted
electrical safety standard for laboratory instruments).

Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.

©2012 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Horizontal System iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo products will be available at www.thermofisher.com.

4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientificiv Horizontal System

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermoscientific.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
www.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We can

Sales Support

take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.

Certified Service Web Page

Our staff can supply technical information about proper setup, operation or

Service Support

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.

When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific
401 Millcreek Road, Box 649
Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Horizontal System vThermo Scientific

Table of Contents

General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Setting Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Preparing the Gel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Using the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Finishing Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Specifications and Recommended Running Conditions . . . . . . . . . . .4-1

Technical Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Combs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Reagent Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Care and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Optional Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Section 1

Section 2

Section 3

Section 4

Section 5

Section 6

Section 8

Section 7

Horizontal System 1-1Thermo Scientific

Section 1 General Information

The Opossum Multiple Gel system is ideal for teaching labs or labs that
need to run multiple gels simultaneously. Up to six minigels (7cmW x
8cmL) can be run on this versatile system. Along with optional combs the
Opossum can screen up to 144 samples at once (120 with supplied
combs). EasyCast casting design allows the casting of gels without tape or
plugs. UV transmissible gel trays may be placed directly on the
transilluminator for photodocumentation.

Before starting, unpack the unit and inventory your order. If any parts are
missing, contact Technical Services immediately.

Reference the order or catalog number on your invoice and check the
corresponding parts list.

Table 1-1.

Parts List
Buffer Chamber
Combs 10 well comb, Double Sided, 1.0/1.5mm Thick (12)
Super Safe Lid with attached power supply leads
Multiple Gel Caster (2)
Gel Tray Holder
UV Transmissible gel trays (6)

1-2 Horizontal System Thermo Scientific

Section 1

General Information

Figure 1-1. Exploded Parts Diagram

Table 1-1. Specifications

Gel size . . . . . . . . . . . . . . . . . . . . . . .7cmW x 8cmL

Running Buffer . . . . . . . . . . . . . . . . . .Volume 2.3L

Footprint . . . . . . . . .28cm W x 37.5cm L x 10cm H

Power Supply

Leads

Super Safe Lid

Gasket (12)

UVT Gel
Tray (6)

Gel Tray
Holder

Multiple Gel
Caster (2)

Double Sided

Comb (12)

1.0 Thick

1.5 Thick

Buffer
Chamber

Bubble Level

Horizontal System 2-1Thermo Scientific

Section 2 Setting Up

1. For shipping and convenient storage, the gel tray is packaged inside the
unit with the gasketed end gates in
position upon arrival. Lift the casting
tray out of the buffer chamber.

2. To cast gels, place the gel tray(s) into
the external multiple gel caster. The
gasketed sides of the UVT gel tray
form a leak proof seal along the side
of the gel caster.

3. Prepare and pour warm (>60) agarose solution onto UVT gel tray.
Place comb(s) into slots provided on walls of UVT gel tray.

4. One the gel has solidified, place the UVT gel tray (s) into the gel tray
holder.

5. Level the casting chamber using the thumbscrews on each side of the
unit by slowly turning one thumbscrew at a time and lining up the
bubble in the level with the center circle. Place the gel tray holder in
the buffer chamber, add buffer, remove combs, load samples and run
the gel.

Figure 2-1. Casting Tray

Figure 2-2. Pour Agarose

Figure 2-3. Place Combs