Thermo Scientific 310, 320, 310 Series, 311, 321 User Manual

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User Manual

Forma Direct Heat CO2 Incubator

Model 310 Series

Operatign and Maintenance Manual 7010310 Rev. 16

Thermo ScientificThermo Scientific

Preface

Model 310 Series DH Incubator i

Preface

Model CO2 Sensor* Voltage**

310 T/C 115

311 T/C 230

320 IR 115

321 IR 230

350† T/C 115

351† T/C 230

360† IR 115

361† IR 230

* T/C is a thermal conductivity sensor.

IR is an infrared sensor.

** All units are 50/60 Hz.

† Copper interior

MANUAL NUMBER 7010310

16 31343/IN-4592 10/22/14 New magnetic door switch (exploded drawing, electrical schematics) RoHS ccs

15 29174/IN-4469 3/21/14 Solenoid change - update 203 drawing and schematics (pgs 8-5 & 9-1 to 9-3) ccs

14 30649/IN-4446 1/28/14 Updated electrical schematics - display board ccs

13 29704/IN-4310 3/29/13 Changed wingnut in blower assembly to hex nut (310-202-1-D) ccs

12 28116/IN-4201 5/29/12 Chg’d harness from 190522 to 1900089 - pg 8-6 ccs

11 27990/IN-4179 5/3/12 Added Intended Use to pg ii, updated Specs uniformity to 0.3°C from 0.2 ccs

10 27292/IN-4073 9/28/11 Switcher from 400199 to 400201 kit - pg 8-1 ccs

-- -- 7/13/10 Re-installed “Specs are based on...” on Specification page. Unintentional removal ccs

Thermo Scientificii Model 310 Series DH Incubator

Preface

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance.

s

Warning If this unit is not used in the manner specified in this operating manual, the protection provided by
the equipment design may be impaired. s

Intended Use: The 310 Series Direct Heat Incubators are designed to maintain an optimal environment for
the incubation of tissue and cell samples. These models are designed to maintain temperature and Carbon
Dioxide levels as set by the operator as well as monitoring chamber relative humidity. CO2 is controlled using
either a thermo conductivity cell, or an infra-red sensor. T

The incubators are approved for general purpose use as a Microbiological Incubator (21 CFR 866.2540) and
are also approved use in assisted reproductive procedures for the incubation of ova and embryos (21 CFR

884.6120).

Non-intended Use:

The 310 Series Direct Heat Incubators are not intended for use where electrical or physical

contact with the patient is established.

These incubators are not intended to be operated in potentially explosive environments and are not intended
for use with flammable materials.

Caution All internal adjustments and maintenance must be performed by qualified service personnel.

s

Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related
to the use of this manual.

©2003 Thermo Fisher Scientific. All rights reserved.

CAUTION

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

Thermo Scientific Model 310 Series DH Incubator iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermo.com.

4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientificiv Model 310 Series DH Incubator

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermoscientific.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
www.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We can

Sales Support

take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.

Certified Service Web Page

Our staff can supply technical information about proper setup, operation or

Service Support

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.

When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific
401 Millcreek Road, Box 649
Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific Model 310 Series DH Incubator vThermo Scientific

Preface

Warranty Notes

Information You Should Know Before Requesting Warranty Service

• Locate the model and serial numbers. A serial tag is located on the unit itself.

•For equipment service or maintenance, or with technical or special application inquiries, contact Technical

Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.

Repairs NOT Covered Under Warranty

• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for

temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
a billable service at your location. Calibration after a warranty repair is covered under the warranty.

• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.

• Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:

1) electrical service connection

2) tubing connections

3) gas regulators

4) gas tanks

5) unit leveling

6) room ventilation

7) adverse ambient temperature fluctuations

8) any repair external to the unit

• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.

• Repairs to parts or systems resulting from unauthorized unit modifications.

• Any labor costs other than that specified during the parts and labor warranty period, which may

include additional warranty on CO

2 sensors, blower motors, water jackets, etc.

Model 310 Series DH Incubator viThermo Scientific

Table of Contents

Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2

Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11

Calibration . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrating the T/C CO

2 System . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Calibrating the Infrared CO2 System . . . . . . . . . . . . . . . . . . . . . . . . .2-3

Calibrating Relative Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4

Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Turn the Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Set an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Set a Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2

Enable the Low Temp Alarm to Trip Contacts . . . . . . . . . . . . . . . . .3-2

Set a Low CO

2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3

Setting a High CO2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3

Enable CO

2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-4

Set New Zero Number for T/C CO2 Sensors . . . . . . . . . . . . . . . . . .3-4

Set New Span Number for T/C CO2 Sensors . . . . . . . . . . . . . . . . . .3-5

Set a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5

Enable RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . . .3-6

Enable Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6

Select Primary Tank w/ Gas Guard Option . . . . . . . . . . . . . . . . . . . .3-7

Disable the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7

Communications Address for RS485 . . . . . . . . . . . . . . . . . . . . . . . .3-8

Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . .4-1

Temp Control Failure Alarm TMP CNTR ERR . . . . . . . . . . . . . . .4-2

Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

CO2 SNSR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

IR AUTOZ ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Section 1

Section 2

Section 3

Section 4

vii Model 310 Series DH Incubator Thermo Scientific

Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Disinfecting the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Cleaning the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Cleaning the Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Cleaning the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Reversing the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

HEPA Filter (Factory Installed Option) . . . . . . . . . . . . . . . . . . . . . . .5-8

Replacing the Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8

Major Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9

Electronics Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9

Replacing the Sample Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10

Factory Options . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Connect Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Connect RS485 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Connecting the Analog Output Boards . . . . . . . . . . . . . . . . . . . . . . .6-2

CO

2 Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Humidity Readout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6

Uninterruptible Power Supply Connections . . . . . . . . . . . . . . . . . . .6-7

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Spare Parts .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Electrical Sch

ematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Warranty Information . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Table of Contents

Section 5

Section 6

Section 8

Section 7

Section 9

Section 10

Model 310 Series DH Incubator 1-1Thermo Scientific

Section 1 Installation and Start-Up

Incubator components are described below.

Figure 1-1. Model 310 Series Direct Heat Incubator

• Outer Door - Reversible to opposite swing, see Section 5

• Inner Door - Reversible to opposite swing, see Section 5

• Chamber Gas Sample Port - Used for sampling chamber CO

2 content

using a FYRITE or similar instrument.

• Main Power Switch

• Control Panel - Keypad, Displays and indicators (See Figure 1-2)

• Leveling Legs - Used to level the unit

Note The incubators are stackable. Information follows.

s

1-2 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-up

Silence - Silences the audible alarm.

Alarm Indicator - Light pulses on/off during an alarm condition in the

cabinet.

Mode Select Switch - Used to select Run, Setpoints, Calibration and

System Configuration Modes.

Message Center - Displays system status.

Mode Select Indicators -

Run: Run Menu

Set: Set Points Menu

Cal: Calibrate Menu

Config: Configuration Menu

Up and Down Arrows - Increases or decreases the number values, toggles

between choices.

Enter - Stores the value into computer memory.

Heat Indicator - Lights when power is applied to the heaters.

Temp Display - Program to display temperature continuously, RH con-

tinuously (with RH option), or toggle between temperature and humid­ity (with RH option). See Section 3, Configuration.

Scroll for Parameters Arrows - Moves the operator through the choices

of the selected mode.

CO

2 Inject Indicator - Lights when CO2 is being injected into the incu-

bator.

% CO2 Display - Displays CO

2 percentage continuously.

Control Panel Keys,

Displays & Indicators

Figure 1-2. Control Panel

Model 310 Series DH Incubator 1-3Thermo Scientific

Section 1

Installation and Start-up

The Model 310 Series direct heat incubator has four basic modes which
allow incubator setup: Run, Setpoints, Calibration and System
Configuration.

Run is the default mode which the incubator will normally be in during

operation.

Set is used to enter system setpoints for incubator operation.

Calibration is used to calibrate various system parameters.

Configuration allows for custom setup of various options.

The chart below shows the selections under each of the modes.

1

T/C units only Base Unit Displays

2

IR units only Option Displays

*T/C units only

Scroll for Parameters Arrows: Steps the operator through the parameters

of SET, CAL and CONFIG Modes. The right arrow goes to the next
parameter, the left arrow returns to the previous parameter.

Up Arrow: Increases or toggles the parameter value that has been selected

in the SET, CAL, and CONFIG Modes.

Enter: Must press Enter key to save to memory all changed values.

Down Arrow: Decreases or toggles the parameter values that have been

selected in the SET, CAL and CONFIG Modes.

Silence Key: Press to silence the audible alarm. See Section 4 for alarm

ringback times.

Keypad Operation

RUN SETPOINT CALIBRATION CONFIGURATION
Default Mode Temperature Temp Offset Audible

Overtemp CO2 Cal

1

Access Code

CO2 IR Cal

2

Temp Lo Alarm

RH Cal Temp Relay

CO2 Lo Alarm
CO2 Hi Alarm
CO2 Relay
CO2 Z & S #'s *

RH Lo Alarm
RH Relay
Display Temp
Display Rh
Tank Select
Gas Guard

RS485 Address

Message Center: Displays the system status (Mode) at all times. Displays

SYSTEM OK during normal operation, or alarm messages if the system
detects an alarm condition. See Section 4, Alarms.

Upper and Lower Displays: These 7 segment displays vary depending

upon the options present and the configuration chosen. The upper dis­play can display temp or RH, or toggle between them. The bottom dis­play shows CO

2 continuously.

Warning Single and stacked units must be installed against a wall or
similar structure. Maintain a three-inch clearance behind the incubator for
electrical and gas hook-ups.

s

1. Locate the unit on a firm level surface capable of supporting the unit’s
weight of 205 lbs.

2. Locate the unit away from doors and windows and heating and air
conditioning ducts.

3. Lift the unit only by the sides of the cabinet base . Do not attempt to
lift it by the front and back . This places stress on the outer door
hinges.

Caution When stacking incubators, the direct heat incubator must be the
top unit. Never stack a water-jacketed incubator on top of a Model 310
Series unit.

s

Warning With incubators in a stacked configuration, do not leave both
exterior doors open at the same time.

s

Warning If the units have been in operation, shut them both off and
disconnect from the power source before beginning any service work. s

Note Two stacking brackets (shown at right) are included
in the parts bag shipped with each incubator.

s

1-4 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Displays

Installing the

Incubator

Stacking the Incubators

Figure 1-3.

Stacking Brackets

1. Remove and discard the slotted head
screws on the top of the bottom incubator
(Figure 1-4). Replace with 5/16 HH bolts
and flatwashers included in the stacking
kit. Do not tighten.

2. Remove the kickplate from below the door on the top incubator
(Figure 1-5). Two Phillips screws secure the plate. Note the notches at
the base of the incubator frame.

3. Disconnect the door cable from the connector behind the plate. Lift
the door off the hinge pins and set it aside. The hinge pins are not
attached to the brackets and may fall out when the door is lifted off.

4. Unscrew and remove the leveling feet from the top unit and lift it onto
the bottom unit, offsetting the base of the top unit approximately 2-3
inches behind the screws identified by the arrows in Figure 1-4.

Warning This incubator weighs 205 lbs. Have sufficient personnel
available when lifting. Lift the unit only by the sides of the cabinet base.
Do not attempt to lift it by the front and back as this places stress on the
outer door hinge.

s

5. Align the sides of the top unit with the bottom unit and slide the top
unit forward until the notches in the base of the top unit align with
the bolts in the top of the bottom unit. The flatwashers should slide
over the base frame. Tighten bolts.

6. Remove the four nylon plugs on the lower back of the upper incubator.

7. Insert the stacking brackets into the slots on the back of the control
panel of the bottom unit as shown in Figure 1-6.

Model 310 Series DH Incubator 1-5Thermo Scientific

Section 1

Installation and Start-Up

Stacking (continued)

Figure 1-4. Slotted Head Screws

Figure 1-5. Align

Figure 1-6. Brackets Installed

8. Align the slots in the brackets with the mounting holes on the back of
the top incubator. Secure the brackets with the screws provided in the
stacking kit.

9. Make sure the hinge pins are in the hinge brackets. Slide the door onto
the hinge pins.

10. Secure the door heater cable to the connector below the door and
replace the kick plate on the top unit, taking care not to crimp cable.

11. The stacked incubators are ready for service.

Remove vinyl from shelf channels, duct sheets, and air duct, if present.
Using a suitable laboratory disinfectant, disinfect all interior surfaces
including shelves and shelf supports, door gaskets, blower wheel and CO

2

sensor. Refer to Section 5.

Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user.

s

1. Install the large sheet metal shelf supports with the tabs facing into the
center of the chamber with their slots up. There are no right side or
left side supports. Simply turn one of them to fit the opposite side. Tilt
the shelf supports as they are placed in the chamber so the tops fit into
the top air duct, then guide them into the vertical position. Figure 1-7
shows the support as it would be oriented for right side of chamber.

1-6 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Preliminary Cleaning and

Disinfecting

Installing the Shelves

Figure 1-7. Shelf Channels and Ductwork

Stacking (continued)

2. Note there is no difference in the left side and right side shelf channels.

3. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the shelf support. Pull the shelf channel
forward and engage the channel’s front slot into the shelf support’s
appropriate forward tab. Refer to Figure 1-8.

Locate the opening in the top left corner on the inside of the chamber.
Remove the tape covering the opening on the outside of the unit. Locate
the stopper with filter in the hardware bag. Install the assembly in the
opening inside the chamber. See Figure 1-9.

Figure 1-9. Filter and Sensor Locations

Caution Be careful when handling the filter. The media can be damaged if

it is mishandled.
To avoid damage to the incubator, do not operate the unit without the
HEPA filter in place.

s

1. Remove the filter from the shipping box.

2. Remove the plastic coating from the filter, using caution not to touch
the filter media.

3. Install the filter as shown in Figure 1-9. Refer to Section 5 for HEPA
filter maintenance.

Model 310 Series DH Incubator 1-7Thermo Scientific

Section 1

Installation and Start-Up

Figure 1-8. Channel Ends

Installing (optional) HEPA

Filter

Installing Access Port

Filter

Installing the Shelves

(continued)

HEPA Filter

Temperature

sensor

Access
port filter

Sample Air Filter

Leveling the Unit

1. Remove the filter from the shipping bag.

2. Install the air sample filter assembly to the black hose barb behind the
top duct.

3. Insert the other end of the filter assembly into the larger hole in the air
intake to the blower. Refer to Figure 1-10.

4. Verify that the temperature sensor is installed into the smaller hole in
the intake, as in Figure 1-10.

Check for level by placing a bubble style level on one of the shelves. Turn
the hex nut on the leveler counterclockwise to lengthen the leg or
clockwise to shorten it. Level the unit front-to-back and left-to-right.

See the serial tag on the side of the unit for electrical specifications or refer
to the electrical schematics at the end of this manual.

Caution Connect the incubator to a grounded, dedicated circuit.
The power cord connector is the mains disconnect device for the
incubator. Position the incubator so the unit can be easily disconnected.

s

1-8 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Figure 1-10. View of Chamber Ceiling

Installing the Air Sample

Filter

Connecting the Unit to

Electrical Power

Plug the provided power cord into the power inlet connector on the back
of the cabinet (See Figure 1-11), then into the grounded, dedicated,
electrical circuit.

Figure 1-11. Connections on Unit Back

Fill the humidity pan with sterile pure water to within 1/2” of the top.
Place the pan directly in the center of the incubator floor to ensure best
humidity and temperature response.

For applications requiring abnormally high humidity, the pan should be
placed against the left side wall of the incubator. The ductwork has been
modified to allow the pan to be placed against the wall of the incubator.
In some ambient conditions, this may cause condensation to form in the
chamber.

For best operation of the incubator, sterilized distilled, demineralized or
de-ionized water should be used in the humidity pan. Water purity should
be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range
of 20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88
for measuring water purity.

Distillation systems, as well as some types of reverse osmosis water purity
systems, can produce water in the quality range specified. Tap water is not
recommended as it may contain chlorine, which can deteriorate the
stainless steel. Tap water may also have a high mineral content, which
would produce a build-up of scale in the pan. High purity or ultra pure
water is not recommended as it is an extremely aggressive solvent and will
deteriorate the stainless steel. High purity water has a resistance of above
1M to 18M Ohm. Even high purity water can contain bacteria and
organic contaminants. Water should always be sterilized or treated with a
decontaminant, safe for use with stainless steel as well as safe for the
product, prior to being introduced into the humidity pan.

Model 310 Series DH Incubator 1-9Thermo Scientific

Section 1

Installation and Start-Up

Filling the Humidity Pan

Connecting the Unit to

Electrical Power (cont.)

Caution Distilled or de-ionized water used in the humidity pan must be
within a water quality resistance range of 50K to 1M Ohm/cm to protect
and prolong the life of the stainless steel. Use of chlorinated water, or
decontamination products containing chlorine, will deteriorate the stainless
steel and cause rust, voiding the warranty.

s

Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will have
an adverse effect on the humidity level and CO

2 calibration of the T/C

units.

Warning High concentrations of CO

2 gas can cause asphyxiation! OSHA

Standards specify that employee exposure to carbon dioxide in any eight­hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO

2). The short term exposure

limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate.

s

Warning This incubator is designed to be operated with CO2 gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO

2 should not be connected to this equipment. CO2

gas cylinders have a UN1013 label on the cylinder and are equipped with a
CGA 320 outlet valve. Check the gas cylinder for the proper identification
labels. The CO

2 gas supply being connected to the incubator should be

industrial grade, 99.5% pure. Do not use CO2 gas cylinders equipped with
siphon tubes. A siphon tube is used to extract liquid CO2 from the
cylinder which can damage the pressure regulator. Consult with your gas
supplier to ensure that the CO

2 cylinder does not contain a siphon tube.

Gas cylinders should also be secured to a wall or other stationary object to
prevent them from tipping.
A two-stage CO

2 pressure regulator is required to be installed on the outlet

valve of the gas cylinder. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa), ±5 psig, for proper performance of the
CO

2 control system. A single stage CO2 pressure regulator will not

maintain 15 psig (103.4 kPa) to the incubator as the pressure in the CO

2

cylinder decreases; therefore, a two stage regulator is recommended.
If higher purity CO2 is desired inside the incubator (greater than 99.5%
pure), the pressure regulator should be constructed with a stainless steel
diaphragm, along with specifying the purity of the CO

2 from the gas

supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder.
Consult your facility safety officer to ensure that the equipment is installed
in accordance with the codes and regulations applicable in your area.

s

1-10 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Connecting the CO2 Gas

Supply

Filling the Humidity Pan

(cont.)

The CO2 gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range. The low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa).

The incubator has serrated fittings on the back of the cabinet to connect
the gas supply. Refer to Figure 1-11. The fitting is labeled CO2 Inlet #1
Tank. Make sure that the connections are secured with clamps. Check all
fittings for leaks.

For units having the CO

2 Gas Guard option, refer to Section 6.

With the incubator properly installed and connected to power, the
humidity pan filled, and the unit connected to gas supplies, system
setpoints can be entered. The following setpoints can be entered in Set
mode: Temperature, Overtemperature and CO

2. To enter Set mode, press

the Mode key until the Set indicator lights. Press the right and/or left
arrow keys until the proper parameter appears in the message center. See
Chart 1-1 for more detail.

Model 310 Series incubators have an operating temperature range of 10°C
to 50°C, depending on ambient temperature. The incubator is shipped
from the factory with a temperature setpoint of 10°C. At this setting, all
heaters are turned off. To change the operating temperature setpoint:

1. Press the Mode key until the Set indicator lights.

2. Press the right arrow until “Temp XX.X” is displayed in the message
center.

3. Press the up/down arrow key until the desired temperature setpoint is
displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights for Run mode or
press the right/left arrow keys to go to next/previous parameter.

Model 310 Series DH Incubator 1-11Thermo Scientific

Section 1

Installation and Start-Up

Incubator Start-Up

Setting the Operating

Temperature

Connecting the CO

2 gas

Supply (continued)

Caution The independent overtemp system is designed as a safety to
protect the incubator only. It is not intended to protect or limit the
maximum temperature of the cell cultures or customer’s equipment inside
the incubator if an overtemp condition occurs.

s

Model 310 Series incubators are equipped with a secondary temperature
monitoring system to monitor the air temperature inside the cabinet. This
system is designed as a safety device to turn off all heaters in the event of a
temperature control failure. Temperature control in the incubator will be ±
1° of the overtemp setpoint.

The factory overtemperature setpoint (default) is 40°C. However, the
overtemp can be set up to 55°C in 0.5° increments.

If the incubator’s operating temperature setpoint is set above the overtemp
setpoint, the overtemp setpoint will automatically update to 1°C above the
temperature setpoint. It is recommended that the overtemp setpoint be
maintained at 1°C over the operating temperature setpoint.

To set the Overtemp setpoint:

1. Press the Mode key until the Set indicator lights

2. Press the right arrow until Otemp XX.X is displayed in the message
center

3. Press the up or down arrow key until the desired Overtemp setpoint is
displayed

4. Press Enter to save the setting

5. Press the Mode key until the Run indicator lights or press the right or
left arrow to go to the next or previous parameter.

1-12 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Setting the Overtemp

Setpoint

All T/C CO2 cells are calibrated at the factory at 37°C, high humidity, and
10% CO

2. Therefore, if a temperature setpoint of 37°C has been entered,

the humidity pan has been filled and the CO

2 control is to run between 0-

10% with a T/C CO

2 sensor, the CO2 setpoint may be entered

immediately. Otherwise, it is important to allow the unit 12 hours to
stabilize at the temperature setpoint before entering the CO

2 setpoint.

All models of the incubator have a CO

2 setpoint range of 0.0% to 20.0%.

The incubator is shipped from the factory with a CO

2 setpoint of 0.0%.

At this setting, all CO

2 control and alarms are turned off. To change the

CO

2 setpoint:

1. Press the Mode key until the Set indicator lights.

2. Press the right arrow until “CO2 XX.X” is displayed in the message
center.

3. Press the up/down arrows until the desired CO

2 setpoint is displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights to go to Run mode
or press the right/left arrow keys to go to next/previous parameter.

Model 310 Series DH Incubator 1-13Thermo Scientific

Section 1

Installation and Start-Up

Setting the CO2 Setpoint

1-14 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Model 310 Series DH Incubator 2-1Thermo Scientific

Section 2 Calibration

After the unit has stabilized, several different systems can be calibrated. In
the calibration mode, the air temperature, CO2 and RH levels can be
calibrated to reference instruments. To access the calibration mode, press
the Mode key until the Cal indicator lights. Press the right and/or left
arrow until the proper parameter appears in the message center. See Chart
2-1 at the end of this section for more detail.

Calibration frequency is dependent on use, ambient conditions and
accuracy required. A good laboratory practice would require at least an
annual calibration check. On new installations, all parameters should be
checked after the stabilization period.

Prior to calibration, the user should be aware of the following system
functions. While the unit is in the calibration mode, all system control
functions are stopped so the unit remains stable. Readout of the system
being calibrated will appear on the message center. If no keys are pressed
for approximately five minutes while in calibration mode, the system will
reset to Run mode so control functions are reactivated.

Caution Before making any calibration or adjustments to the unit, it is
imperative that all reference instruments be properly calibrated.

s

Before calibration, allow the cabinet temperature to stabilize. Place the
calibrated instrument in the center of the chamber. The instrument
should be in the airflow, not against the shelf.

Temperature Stabilization Periods

Startup - Allow 12 hours for the temperature in the cabinet to stabilize

before proceeding.

Already Operating - Allow at least 2 hours after the display reaches
setpoint for temperature to stabilize before proceeding.

Calibrating the

Temperature

2-2 Model 310 Series DH Incubator Thermo Scientific

Section 2

Calibration

1. Press the Mode key until Cal indicator lights.

2. Press the right arrow until “TEMPCAL XX.X” appears in the message
center.

3. Press up/down arrow to match display to calibrated instrument.

4. Press Enter to store calibration.

5. Press the Mode key to return to Run or the right/left arrow to go to
next/previous parameter.

Models 310, 311, 350 and 351 have a thermal conductivity (T/C) CO

2

sensor. Thermal conductivity of the incubator atmosphere is not only
effected by the quantity of CO

2 present, but also by the air temperature

and water vapor present in the incubator atmosphere. In monitoring the
effects of CO

2, air temperature and absolute humidity must be held

constant so any change in thermal conductivity is caused by a change in
CO

2 concentration.

Changing temperature or changing from elevated humidity to room
ambient humidity levels will necessitate a re-calibration of the CO

2

control.

T/C CO

2 Sensor Stabilization Periods

Some T/C CO

2 sensors go through an aging period, especially on new

installations. Calibration should be checked on a weekly basis, and
adjusted as necessary. When stabilization occurs, checks may become less
frequent.

Start -Up - The CO

2 sensor has been calibrated at the factory for 37°C

and elevated humidity. Allow the temperature, humidity, and CO

2 levels

in the chamber to stabilize at least 12 hours before checking the CO

2

concentration with an independent instrument.

Presently Operating - Make sure the chamber doors are closed. Allow at
least 2 hours after the temperature and CO

2 displays reach their setpoints

for chamber atmosphere stabilization.

Calibrating the T/C

CO

2 System

Calibrating Temp

(continued)

Model 310 Series DH Incubator 2-3Thermo Scientific

Section 2

Calibration

1. Make sure the stabilization periods outlined above are followed.

2. Sample the chamber atmosphere through the sample port with an
independent instrument. Sample the atmosphere at least 3 times to
ensure accuracy of the instrument.

3. Press the Mode key until the Cal indicator lights.

4. Press the right arrow until “CO2 CAL XX.X” is displayed in the
message center.

5. Press the up /down arrows to change the display to match the
independent instrument.

6. Press Enter to store the calibration.

7. Press the Mode key to return to Run or the right or left arrows to go
to the next/ previous parameter.

Models 320, 321, 360 and 361 have an infrared (IR) CO

2 sensor. Infrared

CO

2 sensors are not effected by chamber atmosphere temperature or

humidity. However, the light detector in the sensor is effected by wide
temperature changes. Therefore, changing temperature setpoints could
necessitate a recalibration of the CO

2. Chamber temperature should be

allowed to stabilize before checking CO

2 concentrations with an

independent instrument, especially on start-up.

All models equipped with an IR/CO

2 sensor have an automatic calibration

which occurs every 24 hours and lasts for 5 to 6 minutes. During
automatic calibration, the CO

2 display is blanked out and HEPA filtered

room air is pumped through the CO

2 sensor. A new CO2 calibration value

is stored in memory for use as the 0.0% CO

2 reference point. The keypad/

control panel is locked during calibration, with the message center reading
“CO2 AUTO CAL”.

IR CO

2 Sensor Stabilization Times

Start-Up- Allow the temperature and the CO

2 of the cabinet to stabilize at

least 12 hours before proceeding.

Presently Operating - Allow CO

2 to stabilize at least 2 hours at setpoint

before proceeding.

Calibrating the

Infrared CO

2 System

Calibrating T/C CO2

System (continued)

Calibrating Relative

Humidity

1. Measure the CO2 concentration in the chamber through the gas
sample port with a FYRITE or other independent instrument. Several
readings should be taken to ensure accuracy.

2. Press the Mode key until the Cal indicator lights.

3. Press the right arrow until “IR CAL XX.X” appears in the message
center.

4. Press the up/down arrow to adjust the display to match the
independent instrument reading.

5. Press Enter to store the calibration.

6. After Enter is pressed, the unit will go into a calibration cycle that will
last 5 to 6 minutes. The control panel is locked during this cycle.

7. Press the Mode key to return to Run mode.

All 310 Series incubators can be equipped with an optional direct readout
relative humidity (RH) sensor. This is a readout only of the chamber
relative humidity level. It does not provide any control of the relative
humidity in the cabinet.

Relative Humidity Stabilization Times

Start-Up - Allow 12 hours for the relative humidity and temperature in the

chamber to stabilize before proceeding.

Already Operating - Allow at least 2 hours after temperature display
reaches setpoint for relative humidity to stabilize before proceeding.

2-4 Model 310 Series DH Incubator Thermo Scientific

Section 2

Calibration

Calibrating the IR CO2

System (continued)

1. Place an accurate independent instrument in the center of the
chamber. Allow at least 30 minutes for RH to stabilize.

2. Press the Mode key until the Cal indicator lights.

3. Press the right arrow key until “RH CAL XX” appears in the message
center.

4. Press the up/down arrow to match the display to the independent
instrument.

5. Press Enter to store the calibration.

6. Press the Mode key to return to Run mode.

If a reliable RH measuring device is not available, the display may be
calibrated to a typical level.

1. Follow the RH stabilization periods outlined previously.

2. With a full humidity pan and stable temperature, the relative humidity
in the chamber will be 95%.

3. Using Step 3-5 of the relative humidity sensor adjustment, adjust the
display to 95%.

This calibration method should be accurate to within 5%.

Model 310 Series DH Incubator 2-5Thermo Scientific

Section 2

Calibration

Calibrating Relative

Humidity (continued)

2-6 Model 310 Series DH Incubator Thermo Scientific

Section 2

Calibration

Model 310 Series DH Incubator 3-1Thermo Scientific

Section 3 Configuration

Several features available in Configuration Mode allow custom setup of the
incubator. These features are listed and described below. All features may
not be necessary in all applications, but are available if needed. To enter
Configuration mode, press the Mode key until the Config indicator lights.
Press the right and/or left arrow until the appropriate parameter appears in
the message center. See Chart 3-1 for more detail.

The audible alarm can be turned on or off. The factory setting is ON.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until Audible XXX is displayed in the message
center.

3. Press up/down arrow to toggle Audible ON/OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to run mode or right/left to go to
next/previous parameter.

A 3-digit Access Code can be entered to avoid unauthorized personnel
from changing the setpoints, calibration, or configuration. A setting of
000 will bypass the access code. The factory setting is 000.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until Acc Code XXX is displayed in the message
center.

3. Press up/down arrow to change the access code.

4. Press Enter to save the access code.

5. Press the Mode key to return to the Run mode or right/left to go to
next/previous parameter.

Set an Access Code

Turn the Audible

Alarm ON/OFF

Enable the Low Temp

Alarm to Trip Contacts

3-2 Model 310 Series DH Incubator Thermo Scientific

Section 3

Configuration

Set a Low Temp

Alarm Limit

The low temp alarm limit (tracking alarm) is the deviation from the
temperature setpoint which will cause a low temp alarm. The low temp
alarm is variable from 0.5° below setpoint to 5° below setpoint. The
factory setting is 1° below setpoint. A minus sign (-) in the display
indicates that the alarm setting is below the setpoint.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until Temp Lo -X.X is displayed in the message
center.

3. Press up/down arrow to change the low temp alarm limit.

4. Press Enter to save the low temp alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

The low temperature alarm can be programmed to trip the remote alarm
contacts. A setting of ON allows it, a setting of OFF will not allow the
temperature alarm to trip the contacts. The factory setting is ON.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until TMP RLY XXX is displayed.

3. Press the up/down key to toggle the setting ON/OFF.

4. Press Enter to save the setting

5. Press the Mode key to return to Run or the right/left arrow key to go
to next/previous parameter.

Model 310 Series DH Incubator 3-3Thermo Scientific

Section 3

Configuration

The low CO2 alarm limit (tracking alarm) is the deviation from the CO2
setpoint which will cause a low CO2 alarm. The setpoint is variable from

0.5% CO

2 below setpoint to 5.0% CO2 below setpoint. The factory

setting is 1.0% CO

2 below setpoint. A minus sign (-) in the display

indicates that the alarm setting is below the setpoint.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until CO2 Lo -X.X is displayed in the message
center.

3. Press up/down arrow to change the low CO

2 alarm limit.

4. Press Enter to save the low CO

2 alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

The high CO

2 alarm limit (tracking alarm) is the deviation from the CO2

setpoint which will cause a high CO2 alarm. The setpoint is variable from

0.5% CO

2 above setpoint to 5.0% CO2 above setpoint. The factory

setting is 1.0% CO

2 above setpoint.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until CO2 Hi X.X is displayed in the message
center.

3. Press up/down arrow to change the high CO

2 alarm limit.

4. Press Enter to save the high CO

2 alarm limit.

5. Press the Mode key to return to run mode or right/left to go to
next/previous parameter.

Set a Low CO2 Alarm

Limit

Setting a High CO

2

Alarm Limit

Set New Zero Number

for T/C CO

2 Sensors

High and Low CO2 alarms can be programmed to trip the remote alarm
contacts. A setting of ON allows it, a setting of OFF will not allow the
CO

2 alarms to trip the contacts. The factory setting is ON.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until CO2 Rly XXX is displayed in the message
center.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run Mode or right/left to go to
next/previous parameter.

If a new T/C CO

2 sensor is being installed, the two numbers on the

factory installed sticker on the T/C cell must be entered to calibrate the
CO

2 in the unit.

Note For the technician’s convenience, a label containing the two numbers
on the T/C cell is affixed inside the electronics drawer. s

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until T/CZ# XXXX is displayed in the message
center.

3. Press up/down arrow to change the zero number to match the sticker.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

3-4 Model 310 Series DH Incubator Thermo Scientific

Section 3

Configuration

Enable CO2 Alarms to

Trip Contacts

Set a Low RH Alarm

Limit

If a new T/C CO2 sensor is being installed, the two numbers on the
factory installed sticker on the T/C cell must be entered to calibrate the
CO

2 in the unit.

Note For the technician’s convenience, a label containing the two numbers
on the T/C cell is affixed inside the electronics drawer.

s

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until T/CS# XXXX is displayed in the message
center.

3. Press up/down arrow to change the span number to match the sticker.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

On units that have the RH option installed, a low RH alarm limit may be
entered. The low RH alarm limit is the %RH in the cabinet which will
cause a low RH alarm. The setpoint is variable from setpoint 0 to 90%
RH The factory setting is 0% RH which disables the alarm.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until RH Lo XX is displayed in the message
center.

3. Press up/down arrow to change the RH low alarm limit.

4. Press Enter to save the RH low alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

Model 310 Series DH Incubator 3-5Thermo Scientific

Section 3

Configuration

Set New Span Number

for T/C CO

2 Sensors

The low RH alarm can be programmed to trip the remote alarm contacts.
A setting of ON will cause this, a setting of OFF will not allow the RH
alarm to trip the contacts. The factory setting is ON.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until RH Rly XXX is displayed in the message
center.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

On units that are equipped with the RH option, the upper seven segment
display on the control panel can be configured to display Temp
continuously, RH continuously, or toggle between Temp and RH. If the
units does not have RH, the upper display will always display temperature.
If temperature is set to ON and the RH is set OFF, temperature will be
displayed continuously. If temperature is set to OFF and RH is set to
ON, RH will be displayed continuously. If both are turned ON, the
display will toggle between the two. The factory setting will default to
toggle mode if the RH option is present.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until Disp Tmp XXX or Disp RH XXX is
displayed in the message center.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

3-6 Model 310 Series DH Incubator Thermo Scientific

Section 3

Configuration

Enable Temp/RH to be

Displayed

Enable RH Alarms to

Trip Contacts

On units equipped with the Gas Guard option, a primary tank can be
selected. The primary tank will be either Tank 1 or 2. The factory setting
is Tank1.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until Tnk Sel X is displayed in the message
center.

3. Press up/down arrow to toggle the setting between 1 and 2.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

On units equipped with the Gas Guard option, the Gas Guard system
may be turned ON, or OFF if not in use. The factory setting is ON.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until Gas Grd XX is displayed in the message
center.

3. Press up/down arrow to toggle the setting ON/OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

Model 310 Series DH Incubator 3-7Thermo Scientific

Section 3

Configuration

Select Primary Tank

w/ Gas Guard Option

Disable the Gas

Guard System

On units that have the RS485 option, direct communication with the
Model 1535 alarm system can be established. Each piece of equipment
connected to the 1535 must have a unique address. An address of 0-24 can
be entered for the incubator. A setting of 0 is an invalid address that the
1535 will ignore. The factory setting for the RS485 address is 0.

1. Press the Mode key until the Config indicator lights.

2. Press the right arrow until RS485 XX is displayed in the message
center.

3. Press up/down arrow to move the RS485 address.

4. Press Enter to save the RS485 address.

5. Press the Mode key to return to Run mode or right/left to go to
next/previous parameter.

3-8 Model 310 Series DH Incubator Thermo Scientific

Section 3

Configuration

Communications

Address for RS485

Model 310 Series DH Incubator 3-9Thermo Scientific

Section 3

Configuration

3-10 Model 310 Series DH Incubator Thermo Scientific

Section 3

Configuration

Model 310 Series DH Incubator 3-11Thermo Scientific

Section 3

Configuration

Table 4-1. Alarms and Descriptions

Model 310 Series DH Incubator 4-1Thermo Scientific

Section 4 Alarms

The Model 310 Series incubator alarm system is shown in the table below.
When an alarm is active, the message appears in the LED message center.
Pressing Silence disables the audible alarm for the ringback period.
However, the visual alarm continues until the incubator returns to a
normal condition. The alarms are momentary alarms only. When an alarm
condition occurs and then returns to normal, the incubator automatically
clears the alarm condition and the message center.

When multiple alarm conditions occur, active messages are displayed in
the message center one at a time, updating at 5 second intervals. Pressing
Silence during multiple alarms causes all active alarms to be silenced and to
ring back in 15 minutes.

The temperature alarms are disabled when the Temp set point is 10°C.
The CO

2 alarms are disabled when the CO2 set point is 0.0%.

Description Message Delay Ringback Relay

No alarm condition exists SYSTEM OK ----- ----- -----

CO2 System Auto Calibrating CO2 AUTO CAL ----- ----- -----

Temp > Otemp Set point SYS IN OTEMP 0 min. 15 min. Yes

Air Temp Sensor Fault (See Section 4) TMP SNSR ERR 0 min. 15 min. No

Temperature Controller Failure (See Section 4) TMP CTRL ERR 0 min. 15 min. YES

CO2 Sensor Fault (See Section 4) CO2 SNSR ERR 0 min. 15 min. No

CO2 Auto Zero Fault (IR option only, See Section 4) IR AUTOZ ERR 0 min. 15 min. No

CO2 Sensor Cannot be Calibrated (IR option only, See Section 4) REPL IR SNSR 0 min. 15 min. No

Inner Door is Open DOOR OPEN 15 min. 15 min. No

CO2 is higher than CO2 High Tracking Alarm CO2 IS HIGH 15 min. 15 min. Programmable

CO2 is lower than CO2 Low Tracking Alarm CO2 IS LOW 15 min. 15 min. Programmable

TEMP is lower TEMP Low Tracking Alarm TEMP IS LOW 15 min. 15 min. Programmable

RH is lower than RH Low Limit Alarm (RH option) RH IS LOW 30 min. 15 min. Programmable

Tank 1 is low, switch to Tank 2 (Gas Guard only) TANK1 LOW 0 min. ---- No

Tank 2 is low, switch to Tank 1 (Gas Guard only) TANK2 LOW 0 min. ---- No

Both tanks are low (Gas Guard only) TANK 1 and 2 LOW 0 min. 15 min. No

- All alarm delays and ringback times are ±30 seconds -

4-2 Model 310 Series DH Incubator Thermo Scientific

Section 4

Alarms

Temp Control Failure

Alarm TMP CNTR ERR

In addition to other safety features designed into Model 310 Series
incubators, a thermostat is also provided to monitor the cabinet’s
temperature. In the unlikely event of a temperature control failure, the
thermostat will turn off all heaters at a cabinet temperature of 65°C,
±10%. This is intended to be a safety feature to protect the incubator, and
is not intended to protect the cell cultures or the equipment inside the
chamber should a temperature control failure occur.

The microprocessor in Model 310 Series incubators continually scans all
available sensors to ensure that they are operating properly. Should an error
be detected, the incubator sounds an alarm and displays the appropriate
message. If such an alarm occurs, contact your local distributor or the
Technical Services department at 740-373-4763 or 1-800-438-4851 (USA
and Canada) or fax [phone].

If the cables or connectors between the main microprocessor board and the
CO

2 sensor or between the CO2 board and the sensor head on the I/R

CO

2 units become loose or disconnected, the CO2 SENS ERR alarm

occurs.

I/R Units

On I/R incubators, the red light on the I/R module (see Figure 5-8 for
module circuit board location) will be lit continuously if communication is
lost between the CO

2 board and the sensor head. The CO2 display will

also be locked at 00.0 without injection. Turning the incubator Off and
On will not clear the alarm. Only proper connection of all components
will correct the alarm.

On incubators equipped with I/R CO

2 control, calibration is done

automatically using an auto zero system. Auto zero occurs once every 24
hours. If, during the auto zero cycle, a CO

2 correction of more than 0.45%

is detected, the IR AUTOZ ERR alarm occurs.

Sensor Fault Alarms

CO2 SNSR ERR

IR AUTOZ ERR

Model 310 Series DH Incubator 4-3Thermo Scientific

Section 4

Alarms

Possible problems which cause this alarm are:

• Auto Zero pump, orifice, filter or tubing will not let air to the sensor.

Possibilities are:

Defective or electrically disconnected air pump

Kinked auto zero vinyl tubing

Disconnected tubing between the air pump and the sensor

Plugged filter or orifice on the auto zero assembly

Defective auto zero circuit

• Cabinet temperature has been increased significantly from a previous
setpoint. For example, the unit was calibrated and operating at 30°C
and the setpoint is increased to 50°C. In this example, calibrating the
CO

2 will correct the alarm.

• High background CO

2 in the laboratory. This could be from leaks in

the tank, regulator or vinyl CO

2 tubing.

• High CO

2 sensor calibration drift. This will require replacement of the

sensor.

• Calibration of the CO

2 system to less than 3% actual CO2. If this has

occurred, contact the Technical Services Department.

IR AUTOZ ERR

(continued)

4-4 Model 310 Series DH Incubator Thermo Scientific

Incubators

PREVENTIVE MAINTENANCE

sensor.

2

, distilled or demineralized water.

 Do NOT use bleach or any disinfectant that has high chloros

 Use sterile

 Avoid spraying cleaner on the CO

 Do not use powdered gloves for tissue cultures.

Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to keep your unit

functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For maximum performance and

efficiency, it is recommended the unit be checked and calibrated periodically by a qualified service technician.

The following is a condensed list of preventive maintenance requirements. See the specified section of the operating manual for further

details.

Thermo Fisher Scientific has qualified service technicians, using NIST traceable instruments, available in many areas. For more information

on Preventive Maintenance or Extended Warranties, please contact Technical Services.

Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required.

Tips for all incubators:

Model 310 Series DH Incubator 4-5Thermo Scientific

Years

Yearly 2

Months







Between experiments



depending on use and environmental conditions

More frequent decontamination may be required,



Preventive Maintenance for Direct Heat Incubators

Action Daily Weekly Monthly 6

, humidity and temperature calibration, as

2

, O

2

Inspect door latch, hinges and door gasket seal.

Check water level in the humidity pan, ½” from top. See “Filling the

Humidity Pan”.

Verify and document CO

applicable. See Calibration section

Perform a complete decontamination procedure. Wipe down interior,

shelves and side ducts with disinfectant. Change or clean blower wheel and

scroll. Clean top duct. Clean humidity pan. Rinse everything well with

sterile distilled water. See “Cleaning the Interior, “Cleaning the Humidity

Pan”.

Change HEPA and gas filters, if applicable (or as needed). See “Installing

Air Sample Filter”, “Installing HEPA Filter”.

Replace AquaTec water cleaning cell, if applicable.

Model 310 Series DH Incubator 5-1Thermo Scientific

Section 5 Routine Maintenance

Warning If the unit has been in service, turn it off and disconnect the

power cord connector before proceeding with any maintenance. s

Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users must check with the
manufacturer that the proposed method will not damage the equipment.

s

Use an appropriate disinfectant. All articles and surfaces to be disinfected
must be thoroughly cleaned, rinsed with sterile water, and rough dried.

Warning Alcohol, even a 70% solution, is volatile and flammable. Use it
only in a well ventilated area that is free from open flame. If any
component is cleaned with alcohol, do not expose the component to open
flame or other possible hazard.
Do not spray T/C sensor with flammable solutions. The internal
temperature of the CO

2 sensor is approximately 150°C when the unit is in

operation. Allow sufficient time for the sensor to cool before cleaning.

s

Caution Do not use strong alkaline or caustic agents. Stainless steel is
corrosion resistant, not corrosion-proof. Do not use solutions of sodium
hypochlorite (bleach) as they may also cause pitting and rusting. s

1. Remove the shelves, HEPA filter (a factory installed option), access
port filter, sample air filter, left and right duct sheets and the top duct.
The top duct requires the two wing nuts to be removed.

2. Set the HEPA filter aside. Wash the shelves, ducts, wing nuts and
stopper with disinfectant. Optional: The shelves, ducts and wing nuts
may be autoclaved.

3. Remove the blower scroll held on by four screws. Remove the blower
wheel and the access port filter. If a new blower wheel and scroll are
going to be used, discard the old ones. If the old ones are being
reused, wash and rinse all parts with disinfectant.

Disinfecting the

Incubator Interior

4. The CO2 sensor may be lowered by unscrewing the retaining ring
around the sensor. This will allow the sensor gasket to be removed,
cleaned and replaced. WIPE the CO

2 sensor, do not spray or soak.

5. Wash the inner door gasket with disinfectant. The inner door gasket
may be removed and/or replaced.

6. Wash the cabinet interior with disinfectant, starting at the top and
working down. Refer to the disinfectant directions for length of time
needed before rinsing. Wash the inner door both inside and out. The
cabinet and door must be rinsed with sterile water until the
disinfectant has been removed. After the cabinet has been rinsed, spray
with 70% alcohol.

7. If the CO

2 sensor gasket was removed, spray it with 70% alcohol and

install it. Install the sample filter.

8. Install the top duct, left and right ducts, inner door gasket, and access
port stopper spraying each with 70% alcohol.

9. Install new HEPA Filter.

10. Install the shelves and spray with 70% alcohol.

Clean the incubator exterior with a damp sponge or soft, well-wrung cloth
and mild detergent dissolved in water. Dry with a soft cloth.

The chamber glass door and the optional independent inner glass doors
may be cleaned using the same disinfectant used on the incubator interior.
It is imperative that they be rinsed with sterile distilled water to remove
the disinfectant residue. The doors should then be dried with a soft cloth.

Precautions in the cleaning and care of the incubator glass doors:

Moisture leaches alkaline materials (sodium, Na) from the surface of the
glass. Evaporation of the moisture concentrates the alkaline and may
produce a white staining or clouding of the glass surface. Cleaning
chemicals with a pH above 9 and heat (autoclaving) accelerate the corro­sion process. Therefore, it is very important to rinse and dry the glass
doors after cleaning. Autoclaving the glass doors should be avoided.

There is no simple method for repairing corroded glass. In most cases,
the glass must be replaced.

5-2 Model 310 Series DH Incubator Thermo Scientific

Section 5

Routine Maintenance

Cleaning the Glass

Doors

Cleaning the Cabinet

Exterior

Disinfecting the

Interior (cont.)

Clean the humidity pan with soap and water and a general use laboratory
disinfectant. Rinse with sterile water and spray with 70% alcohol. The
humidity pan may be autoclaved.

For side-by-side operation or changing lab layouts, the inner and outer
doors are field reversible. The procedure is written from the prospective of
changing the door swing from a left-hand to a right-hand swing. All screw
holes are pre-drilled for reversing the door. The tools required are a
Phillips screwdriver, a 1/8” Allen wrench and a flatblade screwdriver.

The door reversing procedure takes about 30 minutes.

Model 310 Series DH Incubator 5-3Thermo Scientific

Section 5

Routine Maintenance

Reversing the Door

Swing

Cleaning the Humidity

Pan

Figure 5-1. Door Swings

1. Remove the kick plate at the bottom of the cabinet by removing the
two Phillips screws indicated by the black arrows in the illustration.
Disconnect the cable inside the cabinet.

2. Open the outer door and remove it by lifting it off its hinge pins. Place
the door on a padded surface to prevent scratches (Figure 5-2).

Note The hinge pins are not connected to the brackets and may fall out
when the door is lifted off.

s

3. Refer to the illustration of the outer door in Figure 5-2. With a
Phillips screwdriver, remove and save the four nylon screws from the
door hinge mounting holes. The black arrows identify the screw
locations.

4. Remove the door hinges, rotate them 180° and secure them on the left
side of the door. Install the nylon screws into the newly exposed holes.

5. Remove the two plastic clips securing the door heater cable. These clips
will be rotated 180° when installed on the left lower edge of the door
frame.

5-4 Model 310 Series DH Incubator Thermo Scientific

Section 5

Routine Maintenance

Figure 5-2. Remove Two Screws

Reversing the Door

Swing (continued)

6. Rotate the kick plate and attach the door heater cable with the clips as
shown in Figure 5-3. The insert in the illustration shows the proper
positioning of the clips.

7. The incubator and door should look similar to Figure 5-4.

Model 310 Series DH Incubator 5-5Thermo Scientific

Section 5

Routine Maintenance

Figure 5-3. Rotate Kickplate

Figure 5-4. Unit with Detached Door

Reversing the Door

Swing (continued)

8. Refer to Figure 5-5 for the next steps. Remove the nylon screws along
the right side of the cabinet. Remove the outer door hinges. Rotate
them 180° and install them on the right side of the cabinet. Install the
nylon screws in the holes vacated by the hinges.

9. With a 1/8” Allen wrench, remove the door open switch target plate
on the top of the inner door.

10. Remove the door latch striker plate.

11. Hold the door securely and remove the upper hinge bracket on the
cabinet. This hinge bracket will be rotated 180° and be placed in the
lower right corner of the cabinet where indicated.

12. Lift the glass door from the lower hinge and set it aside. Then remove
the lower hinge from the cabinet. This hinge bracket will be rotated
180° and be placed in the upper right corner of the cabinet at where
indicated.

13. Remove the screws and washers from the new striker plate location.
These screws and washers can be threaded into the holes vacated by the
striker plate where indicated.

14. Rotate the striker plate 180° from its original position and attach it to
the cabinet where indicated.

15. Using a flatblade screwdriver, remove the threaded nylon plugs from
the new hinge bracket locations. Install the lower hinge bracket where
indicated.

5-6 Model 310 Series DH Incubator Thermo Scientific

Section 5

Routine Maintenance

Figure 5-5. Component Locations

Reversing the Door

Swing (continued)

16. Rotate the door 180° and place it in the lower hinge bracket. Install
the upper hinge bracket where indicated.

17. Screw the threaded plugs into the holes vacated by the door hinge
brackets where indicated in Figure 5-5.

18. Install the door open switch target plate. Refer to Figure 5-6 and 5-7.
The plate must be positioned exactly 12.5 inches from the outside
edge of the cabinet to the leading edge of the plate. Do not
overtighten.

19. Connect the door heater cable and install the kick plate with the door
heater cable on the right side.

20. Installing the outer door onto the hinge pins completes the door
reversal procedure.

Model 310 Series DH Incubator 5-7Thermo Scientific

Section 5

Routine Maintenance

Figure 5-6. Reversed Components

Figure 5-7. Plate Position

Reversing the Door

Swing (continued)

Replace the HEPA filter routinely on an annual basis, or more often if it
changes color. The filter should also be changed when the chamber is
cleaned.

Warning The electronics section contains hazardous voltages. Only
qualified service personnel should access this area.

s

There are only two replaceable fuses in the incubator. To access the fuses:

1. Turn off the incubator’s power switch and unplug the power cord.

2. Open the outer door to the chamber.

3. Remove the two screws located below the control panel.

4. Lift up on the control panel, then pull it out.

5. Refer to Figure 5-8 for the location of the two fuses.

6. Slide the control panel back in, replace the screws and return the unit
to service. If the fuse(s) blow after restoring power to the incubator,
contact Technical Services Department.

5-8 Model 310 Series DH Incubator Thermo Scientific

Section 5

Routine Maintenance

Replacing the Power

Fuses

Forma

Figure 5-8. Major Components in Electronics Drawer

HEPA Filter (Factory

Installed Option)

CO

CO manifold and

2

supply tank connectors

RS485 and

remote alarm contacts

sensor board

output board

CO sensor

IR CO

Analog

microbiological

IR_T/C

2

2

2

filter

CO gas

solenoid

Humidity

sensor

Overtemp
thermostat

Power

transformer,

export

2

Temp
probe

CO

injection

port

Micro board

Accessory

outlet

75W max.

Sample port into chamber

Blower

2

motor

Fuses

Power switch

Filtered line
cord connector

Main power transformer

Power supply board

Electronics Section

Expanded descriptions for some of the components indicated in Figure 5-8
follow.

Power Switch - Located beneath the control panel. The outer door must

be open to access the switch.

RS 485 and Remote Alarm Contacts - Connectors and auxiliary wire

through port

CO

2 manifold and supply tank connectors - Part of the Gas Guard sys-

tem, a factory installed option.

Humidity sensor (optional)

Analog output board - 4-20 mA, 0-1 Volt, 0-5 Volt

1. Turn off the incubator’s power switch and unplug the power cord.

2. Remove the two screws from the front of the cabinet top and the two
screws in the uppermost corners of the back panel.

3. Slide the top about an inch to the rear and lift it off.

Note There are small locking tabs in the back which will be bent if the top
is lifted off without sliding it first to the back.

s

Model 310 Series DH Incubator 5-9Thermo Scientific

Section 5

Routine Maintenance

Major Components

Fuse Voltage Manufacturers Part #

Amperage

Rating

Rupture Speed

IEC Letter

Code

115 VAC ACC BUSS GMC - 1.0A 1.0 Amp Time-Lag T

230 VAC ACC BUSS BK-GMC-500ma 0.5 Amp Time-Lag T

24 VAC Door Heat Schurter 034.3124 5.0 Amp Time-Lag T

Table 5-1. Fuses

Replacing the Power

Fuses (cont.)

1. Connect one end of the sample port filter to the ceiling port.

2. Connect the other end of the filter to the back of the blower fan inlet.

Figure 5-9. Air Sample Filter

5-10 Model 310 Series DH Incubator Thermo Scientific

Section 5

Routine Maintenance

Replacing the Sample

Air Filter

Section 6 Factory Options

Descriptions for connections to external equipment follow.

A set of relay contacts is provided to monitor alarms through an RJ11
telephone style connector on the back of the cabinet. Refer to Figure 6-3
for the location of the alarm connector. The 12-foot telephone cord (P/N

190388) and RJ11-to-screw terminal conversion box (190392) are
available through our service department.

The remote alarm provides a NO (normally open) output, an NC
(normally closed) output and COM (common). Refer to Figure 6-1.

The contacts will trip on a power outage or an overtemperature condition.
The contacts may also be programmed to trip or not trip on temperature
alarms, CO

2 alarms, and RH alarms. See Section 3, Configuration Mode.

Figure 6-1. Remote Alarm Output

Model 310 Series DH Incubator 6-1Thermo Scientific

Connect Remote

Alarm Contacts

CAUTION

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

All incubator models can be purchased with the RS485 communications
option (P/N 190523). This option allows the incubator to be directly
connected to a Model 1535 alarm system without the use of a
communications module. A junction box is provided with each RS485
option. See Figure 6-2 for wiring details. Figure 6-3 shows the RS485
connector location on the back of the incubator cabinet.

To allow the incubator and the 1535 to communicate, an address must be
allocated on the 1535. Refer to Section 5.8 of the 1535 operating manual.
The same address number must be assigned to the incubator. Refer to
Section 3 of this manual.

Warning The electronics section contains hazardous voltages. Only
qualified personnel should access this area.

s

The analog output board option (P/N 190512, 190543, 190544) allows
the incubator to output analog signals representing the air temperature of
the cabinet, CO

2 content, and relative humidity, depending upon which

systems are in the incubator. There are three different analog output board
options available : 0-1V, 0-5V, or 4-20mA signals. Negative display
readings will output 0V. The outputs do not have isolated grounds. Refer
to Table 6-1 for output specifications of the three boards.

For the 0-1V and 0-5V boards, the recording device must supply a load
>/= 1000 ohm. For the 4-20mA board, the recording device must supply a
load of </= 100 ohm.

6-2 Model 310 Series DH Incubator Thermo Scientific

Section 6

Factory Options

Connect RS485

Interface

Connecting the

Analog Output Boards

Figure 6-2. RS485 Wiring

Model 310 Series DH Incubator 6-3Thermo Scientific

Section 6

Factory Options

To wire in the analog output board, a shielded 22 gauge, 3-conductor wire
(P/N 73041) is recommended, with the maximum length being 50 feet
(15.2m). This wire is readily available from other vendors including Alpha
P/N 2403 and Deerborn P/N 972203.

Figure 6-3. Auxiliary Through-Port on Back Panel

Caution Accuracy of the output at the board terminal strip to the

incubator display is ±1 unit. There is no calibration from the incubator.
Calibration to the incubator display must be at the instrument connected
to the output board.

s

To install the analog board:

1. Turn off the incubator and unplug it from the wall outlet.

2. Remove the top of the electronics section. Refer to Section 5 of this
manual.*

3. Locate the Analog Output board, Figure 6-4 and Figure 5-8.

* Alternate access to the electronics is to open the outer door and remove the two
screws in the upper corners of the cabinet just under the display top. Lifting up
the top section, the electronics drawer can be pulled out to the limit of the
internal wiring.

190512 4-20 mA
Output Scaling
4-20 mA Equals

190544 0-1V
Output Scaling
0-1 V Equals

190543 0-5V
Output Scaling
0-5V Equals

Temperature 0.0-100.0°C 0.0-100.0°C 0.0-100.0°C

RH 0-100 %RH 0-100 %RH 0-100 %RH

CO2 0.0-100.0 %CO2 0-100.0 %CO2 0-100.0 %CO2

Table 6-1. Analog Output Boards

Connect Analog

Output Boards (cont.)

RS 485

ALARM CONTACTS
30V MAX / 1A MAX

#2

#1

CO inject

CO inject

ACCESSORY

75 WATTS MAX.

22

15 p.s.i. Regulated

15 p.s.i. Regulated

(103.4 kPa)

(103.4 kPa)

ALARM CONTACTS
30V MAX / 1A MAX

RS 485

Figure 6-4. Connector Terminals

4. Each system monitored (Temp, CO

2, RH) requires two conductors

(refer to Figure 6-4). Feed the wires through the auxiliary wire
through-port on the back panel. This port is a circular fitting adjacent
to the remote alarm and RS485 connectors as illustrated in Figure 6-3.
The wire routing is shown by the black arrows in Figure 6-5.

5. Strip the ends of each conductor and wire it to the appropriate
terminals of connectors J2 and/or J3 on the analog board. Refer to
Figure 6-4.

6. When wiring is completed, slide in the electronics drawer or the
replace the sheet metal cabinet top.

7. Replace the screws removed earlier and return the unit to service.

6-4 Model 310 Series DH Incubator Thermo Scientific

Section 6

Factory Options

Figure 6-5. Wire Routing

Connect Analog

Output Boards (cont.)

Warning CO2 can be lethal in high concentrations. Refer to warnings in
Section 1 of this manual. s

The Model 310 Series incubators can be equipped with a built-in Gas
Guard system (P/N 190640) that will operate with a CO

2 gas supply. The

Gas Guard uses two pressure switches to continuously monitor the
pressures of two independent CO

2 supplies and automatically switches

from one supply to the other when the supply pressure drops below 10
psig (0.690 bar). The Gas Guard’s design does not facilitate use by
multiple incubators.

The CO

2 gas supplies must be equipped with two-stage pressure regulators

with gauges. The high pressure gauge at the tank should have a 0-2000
psig range and the low pressure gauge should have a 0-30 psig range. The
gas supply to the incubator must be maintained at 15 psig (1.034 bar), ±5
psig. Gas pressures below 15 psig will cause nuisance alarms to occur on
incubators equipped with the built-in Gas Guard.

The CO

2 inlets for the Gas Guard are located on the back of the cabinet.

Using 1/4” ID tubing, connect one of the CO

2 supply tanks to the fitting

labeled CO2 Inlet #1 Tank. Connect the second CO

2 supply tank to the

fitting labeled CO2 Inlet #2 Tank. Install 3/8” hose clamps to secure the
1/4” ID tubing to the fittings on the back of the drawer. (Refer to Figure
6-6.)

The built-in Gas Guard is turned ON when shipped from the factory. In
addition, the Tank Sel for the Gas Guard is specified as Tank 1 when
shipped. Refer to Section 3, Configuration, to de-activate the Gas Guard
or change the Tank Sel from #1 to #2. If the Gas Guard system is not
used, the incubator will function normally by supplying CO

2 from the

supply tank connected to Inlet #1.

Model 310 Series DH Incubator 6-5Thermo Scientific

Section 6

Factory Options

Figure 6-6. Connect Gas Supplies

De-activating the Gas

Guard

Connecting the CO

2 Gas

Supplies

CO2 Gas Guard

With the Gas Guard in operation, the incubator will use the gas supplied
by the tank connected to Inlet #1 until the pressure drops below 10 psig
(0.690 bars). At this time, the Gas Guard automatically switches to gas
supplied through CO2 Inlet #2.

In addition, the incubator automatically changes the Tank Sel in
Configuration Mode from 1 to 2 to indicate that the incubator is now
using gas supplied through Inlet # 2. If the gas supply to Inlet #1 is
replenished, the incubator will continue to operate using the gas supplied
through Inlet #2 unless the operator changes the Tank Sel from #2 to #1
through Configuration Mode. Refer to Section 3, Configuration.

Audible and visual alarms occur on the control
panel when the gas guard switches from one supply
to the other. The audible alarm sounds until the
operator presses the Silence key on the control
panel. The visual alarm in the Message Center reads
Tank 1 Low while the audible alarm is sounding,
but the message is removed when the operator
presses the Silence key. However, the ‘Tank Low’
indicator on the control panel stays lighted until the
condition is resolved. The unit operates normally.

Caution Both the audible and visual alarms described above do not ring
back once the Silence key is pressed.

s

If the Gas Guard system does not detect an adequate gas supply at CO2
Inlet #1 or Inlet #2, a visual and audible alarm will again occur on the
control panel. The visual alarm in the Message Center will read Tank
1&2 Low. The audible alarm will continue to ring until the Silence key is
pressed. The audible alarm will ring back every 15 minutes after the alarm
is silenced if the Gas Guard continues to detect that both gas supply
pressures are below 10 psig (0.690 bars).

The 310 Series incubators can be equipped with a humidity sensor (P/N

190643) to monitor the relative humidity inside the chamber. The sensor
is mounted to the top air duct and provides a signal that is displayed in
1% increments on the control panel. The humidity readout can be
displayed continuously or toggles with the temperature readout. In
addition, a low alarm limit can be set on the humidity readout, which will
detect when the humidity pan runs dry. Refer to Section 3,
Configuration.

6-6 Model 310 Series DH Incubator Thermo Scientific

Section 6

Factory Options

Humidity Readout

Operation of the CO2 Gas

Guard

Figure 6-7. Silence Key

• Water level in the humidity pan

• Frequency of door openings

• The humidity pan located on bottom of chamber versus a shelf.

Caution Incubators equipped with a Thermal Conductivity CO

2 sensor

rely on a constant level of relative humidity in order to accurately measure
and control the CO

2 concentration in the incubator. s

The sensor is capable of measuring relative humidity from 10% to 100%
with an accuracy of ±5% above 90%. See Section 2, Calibration, for
details on calibrating the humidity readout.

Warning The Uninterruptible Power Supply is not rated for life-support
applications.

s

The optional Uninterruptible Power Supply ([UPS] P/N 270078 and

270082) provides a back-up power source for the incubator. When the
batteries are fully charged and the incubator operating at 37°C in an 18°C
ambient temperature, the UPS provides approximately one hour of
uninterrupted power during a power failure. A higher operating
temperature decreases this back-up time, a higher ambient temperature
increases it. This estimated time assumes that the incubator will be already
at its operating temperature and that the incubator door will not be
opened during the back-up period.

The remote alarm contacts will not trip during a power failure while
connected to the UPS until battery power is exhausted. There is however,
an audible and visual alarm when the unit switches to battery operation.

The UPS batteries are fully charged when the unit has been connected to
utility power for a minimum of four hours. The UPS power switch does
not have to be on for the batteries to charge. When battery replacement
becomes necessary, refer to the UPS user’s manual.

Caution Use only factory recommended UPS units which produce a pure
sine wave output. Other UPS units could damage the incubator and void
the warranty. Typical facility back-up systems will not effect the efficiency
of the incubator.

s

Model 310 Series DH Incubator 6-7Thermo Scientific

Section 6

Factory Options

Uninterruptible Power

Supply Connections

Humidity Level Factors

Humidity Readout

Accuracy

1. Plug the incubator line cord into one of the receptacles on the back of
the UPS. Then plug the UPS line cord into a suitable wall outlet.

2. Turn the UPS power switch on.

Use the line cord supplied with the incubator to connect the UPS to a
suitable wall outlet, and the UPS coupler to connect the incubator to the
UPS.

Warning The Uninterruptible Power Supply contains potentially
hazardous voltages even when not connected to line voltage.
Do not attempt to disassemble UPS as it contains no user-serviceable parts.
Repairs must be performed by factory trained service personnel.

s

6-8 Model 310 Series DH Incubator Thermo Scientific

Section 6

Factory Options

Connecting UPS

P/N 270078, 115V, 50/60Hz

Connecting UPS

P/N 270082, 230V, 50/60Hz

Section 7 Specifications

* Specifications are based on nominal voltages of 115V or 230V in ambient of 22°C

to 25°C.

Temperature

Control . . . .±0.1°C Microprocessor PID Control

Setpoint . . . . . . . . . . .Digital - Touch pad, 0.1°C

Range . . . . . . . . . . . .+5°C above ambient to 50°C

Uniformity . . . . . . . . . . . . . . . . . .±0.3°C @ +37°C

Tracking alarm . . User programmable (low) indicator

Overtemp . .Tracking, user programmable, action, and indicator

Display . . . . . . . . .Digital, LED, 0.1°C increments

Temperature Safety

Type . . Extreme temperature safety, action, and indicator

Sensor . . Thermostat, independent of temperature control system

Indicator . . Message center, audible and visual alarms

Relative Humidity

Control . . . . .Humidity pan - natural vaporization

Humidity with pan . . . . . . . . . . .95% RH at 37°C

Display . . . . . . . . . . . . .Optional in 1% increments

Alarm . . . . . . .Low RH with optional RH monitor

CO

2

Control . . . . .±0.1% microprocessor PID control

Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . .T/C or IR

Readability . . . . . . . . . . . . . . . . . . . . . . . . . . .0.1%

Range . . . . . . . . . . . . . . . . . . . . . . . . . . . .0 to 20%

Inlet pressure . . . . . . . . . . . . . . . . .15 psig, ±5 psig

Display . . . . . . . . . .Digital LED, 0.1% increments

Model 310 Series DH Incubator 7-1Thermo Scientific

7-2 Model 310 Series DH Incubator Thermo Scientific

Section 7

Specifications

Shelves

Dimensions . . . . . . . .18.5” x 18.5” (47cm x 47cm

Construction . . Stainless steel (belt sanded, both sides)

Surface area . . . . . .2.4 sq. ft. (0.22 sq. m) per shelf

Max. per chamber . . . . . . . . .38.4 sq ft (3.6 sq. m)

Loading . . . .35 lbs (16kg) slide in and out, 50 lbs (23kg) stationary

Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4

Maximum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

Construction

Interior volume . . . . . . . . . . . . . . . . . . . .6.5 cu. ft.

Interior . . . . . .Type 304 stainless steel shiny finish

Exterior . . . . . . . . . . . . . . . .18 gauge cold roll steel

Outer door gasket . . Four-sided molded, magnetic vinyl

Inner door gasket . . . . . . .Feather-edge, removable

Insulation . . . . . . . . . . . . . . . . . . . . . . . .Fiberglass

Fittings

Access port . . . .1-1/4 inch removable silicone plug

CO

2 inlet . . . . . . . . . . . . . . . . . . . . . . .1/4” barbed

Sample port . . . . . . . . . . . . .Front mounted barbed

Electrical

115 Volt models -

90-125VAC, 50/60 Hz, 1PH, 2.4 FLA

230 Volt models -

180-250, 50/60 Hz, 1 PH, 2.0 FLA

Power switch/circuit breaker . . . . .2 Pole, 6.0 Amp

Accessory outlet . . Voltage equal to the cabinet input,
75 Watts maximum, 0.5ma leakage current

Alarm contacts . . Deviation of temperature, CO

2, power, NO and NC

Unit BTU Output

115/230V . . . . . . . . . . . . . .344 BTUH (100 watt)

Dimensions

Exterior . . . . . . . . .26.0” W x 38.5” H x 25.0” F-B

Interior . . . . . . . . .21.4” W x 26.8” H x 20.0” F-B

Model 310 Series DH Incubator 7-3Thermo Scientific

Section 7

Specifications

Weight

Net Weight Stainless Steel . . . . . . . . . . . . .205 lbs

Net Weight Copper . . . . . . . . . . . . . . . . .230 lbs.

Options/Accessories

HEPA filter . . Factory installed: Stock no. 190858

Battery back-up . . Free Standing: Stock no. 270078, 120VAC, 60Hz

Stock no. 270082, 220VAC, 50Hz

Humidity . . Factory installed: Stock no. 190463 - provides humidity

readout and low alarm

Inner door kit . . Customer installed: Stock no. 190650 - independent

. . . eight separate glass doors

Built-in Gas Guard . . Factory installed: Stock no. 190640 - CO

2

only, automatically switches supply tanks

Recorders . . Customer installed: Standard recorders used on water-

jacket incubators

Decontamination kits

HEPA filter, blower assembly, inner door gasket, etc. ­ Stock no. 190868 (with HEPA filter),

Stock no. 190869 (without HEPA filter)

Copper Options

Interior ductwork .Solid copper: Stock no. 190725

Perforated shelfCustomer Installed: Stock no. 224166 - solid copper

Humidity PanCustomer Installed: Stock no. 237015 - solid copper

Copper interior kitFactory installed: Replace stainless steel duct work,
shelves, and

humidity pan with solid copper Stock no. 190870

Optional Data Outputs

Analog Factory installed: Stock no. 190544 - 0-1 volt

Analog Factory installed: Stock no. 190543 - 0-5 volt

Analog Factory installed: Stock no. 190512 - 4-20mA

Digital Factory installed: Stock no. 190523 - RS-485

(Compatible with Model 1535 Alarm Monitor only)

Certifications

Refer to the Declarations of Conformity at the back of this manual

Safety Specifications

Indoor Use Only

Altitude - 2,000 meters

Temperature - 5°C to 40°C

Humidity - 80% RH at or below 31°C, decreasing linearly to 50% RH
at 40°C

Mains Supply Fluctuations - Mains supply voltage fluctuations not to
exceed ±10% of the nominal voltage

Installation Category II

1

Pollution Degree 2

2

Class of Equipment I

1

Installation Category (overvoltage category) defines the level of transient overvoltage
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its overvoltage protection means. For example, in CAT II which is
the category used for instruments in installations supplied from a supply comparable to
public mains such as hospital and research laboratories and most industrial laboratories,
the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V
supply.

2

Pollution Degree describes the amount of conductive pollution present in the operating
environment. Pollution Degree 2 assumes that normally only non-conductive pollution
such as dust occurs with the exception of occasional conductivity caused by condensation.

7-4 Model 310 Series DH Incubator Thermo Scientific

Section 7

Specifications

Section 8 Spare Parts

Model 310 Series DH Incubator 8-1Thermo Scientific

Part Number Description

190699 Removable feather gasket

190670 Duct sheet and shelf channel kit

190772 Micro board, (main control)

230135 Fuse, 1A, TD, 5 x 20mm

230120 0.5A fuse, TD, 5 x 20mm (accessory outlet)

230161 Fuse, 5A, 5 x 20mm

156126 Motor, 2-pole, 115VAC, 50/60 Hz

190793 Display board

290184 Probe, 2252 Ohm/25°C, 1/8 x2

770001 Disposable filter, 99.97 (CO2 line, air sample)

760175 HEPA filter, 5.5” diameter

760199 HEPA2 VOC Filter Replacement Kit (includes 760200 HEPA2, 117036 silicone plug, 101018 o-ring)

1900067 Filter Replacement Kit (includes [2] 770001 filter and 760175 HEPA)

1900094 HEPA2 VOC Filter Replacement Kit (760200 HEPA2, 190985 access port filter, 770001 filter)

224175 Shelf

190868 Decontamination kit with HEPA filter

190869 Decontamination kit without HEPA filter

130077 Stopper with 3/8” hole

290090 Thermal conductivity type CO2 sensor

190885 Infrared type CO2 sensor w/ auto zero

250087 CO2 valve with fittings

290154 RH sensor assembly (RH display option)

190883 Sample port filter assembly

190661 IR sensor filter

190985 Filtered air exchange assembly

230153 6A, DPDT circuit breaker switch (power)

400201 40W Switcher kit

420096 130VA transformer, int’l, 12/24V S

194021 34 position control to display ribbon cable

420097 43VA transformer (230VAC units only)

360213 Pressure switch (Gas Guard option)

250121 Gas valve (Gas Guard option)

190512 4-20mA output board

190543 0-5V output board

190544 0-1V output board

8-2 Model 310 Series DH Incubator Thermo Scientific

Section 8

Spare Parts

Model 310 Series DH Incubator 8-3Thermo Scientific

Section 8

Spare Parts

8-4 Model 310 Series DH Incubator Thermo Scientific

Section 8

Spare Parts

Assembly Drawing

Model:

310/3110 Series

2

CO Incubator

310-202-1-D Rev. 6

Page 1 of 1

Model 310 Series DH Incubator 8-5Thermo Scientific

Section 8

Spare Parts

8-6 Model 310 Series DH Incubator Thermo Scientific

Section 8

Spare Parts

Model 310 Series DH Incubator 8-7Thermo Scientific

Section 8

Spare Parts

Model 310 Series DH Incubator 9-1Thermo Scientific

Section 9

Electrical Schematics

Electrical Schematic

Model:

115V Air Jacket

Incubator

310-70-0-D Rev. 6

Page 1 of 3

9-2 Model 310 Series DH Incubator Thermo Scientific

Section 9

Electrical Schematics

Electrical Schematic

Model:

115V Air Jacket

Incubator

310-70-0-D Rev. 6

Page 2 of 3

Model 310 Series DH Incubator 9-3Thermo Scientific

Section 9

Electrical Schematics

Electrical Schematic

Model:

115V Air Jacket

Incubator

310-70-0-D Rev. 6

Page 3 of 3

9-4 Model 310 Series DH Incubator Thermo Scientific

Section 9

Electrical Schematics

Electrical Schematic

Model:

311 & 321

Air Jacket Incubator

311-70-0-D Rev. 5

Page 1 of 3

Model 310 Series DH Incubator 9-5Thermo Scientific

Section 9

Electrical Schematics

Electrical Schematic

Model:

311 & 321

Air Jacket Incubator

311-70-0-D Rev. 5

Page 2 of 3

9-6 Model 310 Series DH Incubator Thermo Scientific

Section 9

Electrical Schematics

Electrical Schematic

Model:

311 & 321

Air Jacket Incubator

311-70-0-D Rev. 5

Page 3 of 3

Model 310 Series DH Incubator 10-1Thermo Scientific

Section 10

Warranty Information

ISO

9001

REGISTERED

Rev. 4 4/09

2 sensor (IR) will be warranted for a second year, parts only. The CO2

THERMO FISHER SCIENTIFIC DIRECT HEAT INCUBATOR WARRANTY USA*

The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows shipping time so the warranty will go

into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first

year warranty period.

During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense,

including labor. In addition, after the initial warranty period of one year the CO

sensor (T/C) will be warranted for four additional years for a total of five years, parts only. Installation and calibration is not covered by this warran-

ty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to performance of any repairs.

Expendable items, glass, filters and gaskets are excluded from this warranty.

Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the com-

ponent part beyond the original warranty period. The Technical Services Department must give prior approval for return of any components or

equipment. At Thermo's option, all non-conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destina-

tion.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO

WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for

any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction

manuals carefully detail equipment installation, operation and preventative maintenance.

If equipment service is required, please call your Technical Service Department at l-800-438-4851 (USA and Canada) or 1-740-373-4763. We're

ready to answer your questions on equipment warranty, operation, maintenance, service and special applications. Outside the USA, contact your local

distributor for warranty information.

10-2 Model 310 Series DH Incubator Thermo Scientific

Section 10

Warranty Information

ISO

9001

REGISTERED

Rev. 4 4/09

sensor (IR) will be warranted for a second year, parts only. The

2

THERMO FISHER SCIENTIFIC INTERNATIONAL DIRECT HEAT INCUBATOR WARRANTY*

The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows shipping time so the warranty will go

into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first

year warranty period.

sensor (T/C) will be warranted for four additional years for a total of five years, parts only. Installation and calibration is not covered by this

2

During the first year component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense,

excepting labor. In addition, after the initial warranty period of one year the CO

CO

warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to performance of any

repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.

Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the com-

ponent part beyond the original warranty period. The Technical Services Department must give prior approval for return of any components or

equipment. At Thermo's option, all non-conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destina-

tion.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO

WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for

any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction

manuals carefully detail equipment installation, operation and preventive maintenance.

If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or 1-740-373-4763. We're

ready to answer your questions on equipment warranty, operation, maintenance, service and special application. Outside the USA, contact your local

distributor for warranty information.

Thermo Fisher Scientific
401 Millcreek Road
Marietta, Ohio 45750
United States

www.thermofisher.com