Thermo Scientifi? TriPlus 500 User Manual
Thermo Scientific
TriPlus 500
User Guide
Headspace Sampler
P/N 31716106 Revision A January 2019
© 2019 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Scientific™ TriPlus™ 500 Headspace Sampler and TRACE™ 1300 Series GC (TRACE™ 1300 GC,
TRACE™ 1310 and TRACE™ 1300E) are trademarks of Thermo Fisher Scientific; VICI ® is a registered mark
of the Valco Instrument Co. Inc., and VICI AG International. All other trademarks are the property of
Thermo Fisher Scientific and its subsidiaries.
Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history:
Revision A, released January 2019“Original Instructions”
For Research Use Only. Not for use in diagnostic procedures.
TriPlus 500 Headspace Sampler User Guide, PN 31716106, Revision A
Reader’s Survey
fold
fold
Strongly
Agree
The manual is well organized. 1 2 3 4 5
The manual is clearly written. 1 2 3 4 5
The manual contains all the information I need. 1 2 3 4 5
The instructions are easy to follow. 1 2 3 4 5
The instructions are complete. 1 2 3 4 5
The technical information is easy to understand. 1 2 3 4 5
Examples of operation are clear and useful. 1 2 3 4 5
The figures are helpful. 1 2 3 4 5
I was able to operate the system using this manual. 1 2 3 4 5
If not, please comment below. Attach additional sheets if necessary.
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
__________________________________________________________ __________________________________________________________________
Agree Neutral Disagree
Strongly
Disagree
Customer Registration Card
Register now…and receive all the privileges associated with being a Thermo Fisher Scientific product user including customer
support, application reports, and technical reports.
MY ORGANIZATION IS: (Check only one) MY PRIMARY APPLICATION IS: (Check only one)
❏ Commercial (for profit) lab ❏ Analytical
❏ Government lab ❏ Biomedical
❏ Hospital/Clinic ❏ Clinical/Toxicology
❏ Industrial lab ❏ Energy
❏ Research Institute ❏ Environmental
❏ University/College ❏ Food/Agricultural
❏ Veterinary ❏ Forensic/Toxicology
❏ Other______________________ ❏ Pharmaceutical
❏ Research/Education
MY PRIMARY JOB FUNCTION IS: (Check only one)
❏ Administration
❏ Lab management
❏ Operator
❏ Other______________________
❏ Other______________________
Name __________________________________________________Title__________________________________________________________________
Company __________________________________________________ __________________________________________________________________
Address ___________________________________________________ __________________________________________________________________
City/State _________________________________________Postal Code __________________________________________________________________
Country ___________________________________________________ __________________________________________________________________
Telephone_______________________________________________ Ext. __________________________________________________________________
Serial Number __________________________________ Date purchased __________________________________________________________________
Fold and mail or e-mail to:
Editor, Technical Publications
Thermo Fisher Scientific S.p.A.
Strada Rivoltana km 4
20090 Rodano (MI)
Italy
Editor, Technical Publications
Thermo Fisher Scientific CMD GC-GC/MS
2215 Grand Avenue Parkway
Austin TX 78728-3812
Unites States of America
Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
• installation
• re-calibration
•changes and repairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, 20090 Rodano - Milan - Italy — Tel: +39 02 950591 - Fax: +39 02 9505276
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
Thermo Fisher Scientific declares, under sole responsibility, that the product as originally delivered complies with
the requirements of the following applicable European Directives and carries the CE marking accordingly:
• Machinery Directive: 2006/42/EC
• EMC Directive: 2014/30/EU
• RoHS Directive: 2011/65/EU
… and conforms with the following product standards:
Compliant with applicable directives:
• Machinery Directive: 2006/42/EC
• EMC Directive: 2014/30/EU
• RoHS Directive: 2011/65/EU
Compliant with product standards:
EMC
– EN 61326-1:2013, IEC 61326-1:2012
– FCC rules: CFR no. 47 Part 15 Subpart B Section 15.107 and 15.109
Safety
– EN 61010-1:2010, IEC 61010-1:2010
– EN 61010-2-010: 2014, IEC 61010-2-010: 2014 (TriPlus 500 HS only)
– EN 61010-2-081: 2015, IEC 61010-2-081: 2015
– UL 61010-1:2012/R:2016-04
– UL 61010-2-010:2015 (TriPlus 500 HS only)
– UL 61010-2-081:2015
– CAN/CSA C22.2 No. 61010-1:2012/U2:2016-04
– CAN/CSA C22.2 No. 61010-2-010:2015 (TriPlus 500 HS only)
– CAN/CSA C22.2 No. 61010-2-081:2015
Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant
to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environment. This equipment generates, uses,
and can radiate radio frequency energy and, if not installed and used in accordance with instruction manual,
may cause harmful interference to radio communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case the user will be required to correct the interference at his own
expense.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO THE
FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
WEEE Directive
2012/19/EU
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union
(EU) Member State, and these companies should dispose of or recycle this product. See www.thermofisher.com/
rohsweee for further information on Thermo Fisher Scientific compliance with these Directives and the recyclers in
your country.
Directive DEEE
2012/19/EU
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de
l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la
conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les
produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont
disponibles sur www.thermofisher.com/rohsweee.
WEEE Direktive
2012/19/EU
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten
getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information
über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise,
die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter
www.thermoscientific.com/rohsweee.
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Power Rating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Safety Information and Warnings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
General Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Electrical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Other Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
Working with Toxic or other Harmful Compounds . . . . . . . . . . . . . . . . . .xxiii
Biological Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxiii
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Writing Convention Used in This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Chapter 1 Getting Familiar with Your TriPlus 500 Headspace Sampler . . . . . . . . . . . . . . . . .1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Instrument Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Label Location on the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Incubation Group . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Sample Vial Carousel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Incubation Oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Sampling Path . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Pneumatic Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Electrical Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Status Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
TRACE 1310 GC User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Vial Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Vial Loader Electronic Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xi
Contents
Sample Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Vials Sequence Syntax . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Positioning the Vials in the Sample Tray. . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
About a Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Barcode Reader Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Barcode Label Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Heated/Cooled Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
TriPlus 500 Web Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Chapter 2 Operating Principles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Introduction to the Headspace Technique. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Pneumatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Analytical Cycle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Stand-by . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Leak Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Loop Filling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Chapter 3 Setting Up Through the TRACE 1310 User Interface . . . . . . . . . . . . . . . . . . . . . . . .43
User Interface Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Menu Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Data Entry Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Shortcut Key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Cursor Keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Configuring the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Editing the Instrument Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Performing Samples Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Monitoring the Instrument Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Information Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Chapter 4 Setting Up Through Chromeleon CDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Configuring the TriPlus 500 HS Through Chromeleon CDS . . . . . . . . . . . . . . 58
Hardware Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
HS Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Error Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Editing Method Parameters Through Chromeleon CDS. . . . . . . . . . . . . . . . . . 61
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Loop Filling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Venting and Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
xii TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Contents
Chromeleon HS Sampler Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Vials Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Chapter 5 Setting Up Through TraceFinder CDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
Configuring TriPlus 500 HS Through TraceFinder CDS . . . . . . . . . . . . . . . . . 72
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Hardware Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
User Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
HS Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Error Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Editing Method Parameters Through TraceFinder CDS . . . . . . . . . . . . . . . . . . 75
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Loop Filling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Venting and Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Advanced. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
TraceFinder Road Map Home Page Status Tabs . . . . . . . . . . . . . . . . . . . . . . . . 79
Chapter 6 Method Development . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Usage Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Carrier Gas Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Auxiliary Gas Pressure Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Shaking Conditioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Sample Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Sample Vial Septa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Vial Filling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Vial Closure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Missing and Wrong Size Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Matrix Effects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Principles of Multiple Headspace Extraction (MHE). . . . . . . . . . . . . . . . . . . 86
Processing a Sample Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
User Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Instrument Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Method Developing Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Analytical Troubleshooting General Guidelines. . . . . . . . . . . . . . . . . . . . . . . . 102
Chapter 7 Using the TriPlus 500 Web Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xiii
Contents
Menu Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Login Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Administration Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Status Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Installation/Tools Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Service Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Manufacturing Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Logging Out of the TriPlus 500 Web Interface . . . . . . . . . . . . . . . . . . . . . . . . 116
Chapter 8 Ordering Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
xiv TriPlus 500 Headspace Sampler User Guide Thermo Scientific
P
Preface
This guide contains detailed information for the use of the Thermo Scientific™ TriPlus™ 500
Headspace Sampler (TriPlus 500 HS).
This guide is intended for frequent or new TriPlus 500 HS users who are experienced at using
automated systems to run existing analytical methods.
Note TriPlus 500 HS must be installed and set up properly before the Operating
Instructions can be used.
This guide is organized as follows:
• Chapter 1, “Getting Familiar with Your TriPlus 500 Headspace Sampler,” provides
information to familiarize you with your TriPlus 500 HS.
• Chapter 2, “Operating Principles,” describes the operating principles of the TriPlus 500
HS.
• Chapter 3, “Setting Up Through the TRACE 1310 User Interface,” contains the
instructions to configure your TriPlus 500 HS and to edit the parameters through the
TRACE 1310 GC user interface (touch screen) or through the TRACE 1310 Virtual
Touch Screen software.
• Chapter 4, “Setting Up Through Chromeleon CDS,” contains the instructions to
configure your TriPlus 500 HS and to edit the parameters through the Thermo
Scientific™ Chromeleon™ Chromatography Data System (CDS).
• Chapter 5, “Setting Up Through TraceFinder CDS,” contains the instructions to
configure your TriPlus 500 HS and to edit the parameters through the Thermo
Scientific™ TraceFinder™ Chromatography Data System (CDS).
• Chapter 6, “Method Development,” provides information for developing a method with
your TriPlus 500 HS.
• Chapter 7, “Using the TriPlus 500 Web Interface,” provides the instructions for using the
TriPlus 500 Web Interface.
• Chapter 8, “Ordering Parts,” contains part numbers for all the consumables and parts
available for your TriPlus 500 HS.
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xv
Preface
About Your System
• “Glossary,” contains definitions of terms used in this guide. It also includes abbreviations,
acronyms, metric prefixes, and symbols.
Contents
• About Your System
• Power Rating
• Contacting Us
• Related Documentation
• Safety Information and Warnings
• Instrument Markings and Symbols
• Safety Information and Warnings
• Writing Convention Used in This Guide
About Your System
Thermo Fisher Scientific systems operate safely and reliably under carefully controlled
environmental conditions. If the equipment is used in a manner not specified by the
manufacturer, the protections provided by the equipment might be impaired. If you maintain
a system outside the specifications listed in this guide, failures of many types, including
personal injury or death, might occur.
The repair of instrument failures caused by operation in a manner not specified by the
manufacturer is specifically excluded from the Standard Warranty and service contract
coverage.
Power Rating
TriPlus 500 HS alone:
• 100-240 Vac; 600 W; 50/60 Hz
Vial Loader
• 24 Vdc through a portable external power supply, level VI efficiency
Input 100-240 Vac; 50/60 Hz; 1.3 A — Output 24 Vdc; Power 90 W; 3.75 A
WARNING You must only use the portable external power supply provided with the
instrument by Thermo Fisher Scientific.
Detailed instrument specifications are in the Product Specifications Sheet.
xvi TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
To find out more about our products
Go to http://www.thermofisher.com for information about our products.
To get local contact information for sales or service
Go to http://www.unitylabservice.com/en/home.html
Related Documentation
In addition to this guide, Thermo Scientific™ provides the following documents for TriPlus
500 HS.
• TriPlus 500 Headspace Sampler Preinstallation Requirements Guide, PN 31716105
Preface
Contacting Us
• TriPlus 500 Headspace Sampler Hardware Manual, PN 31716107
• TriPlus 500 Headspace Sampler Spare Parts Catalog, PN 31716108
To suggest ways we can improve the documentation, follow this link to complete our Reader’s
Survey.
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this manual. The safety and
other special notices appear in boxes.
Special Notices
Notices includes the following:
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xvii
Preface
Safety Alerts and Important Information
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury, instrument damage, or both. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION is intended to prevent improper actions
that might cause personal injury, instrument damage, or both. You can find the following
safety symbols on your instrument, or in this guide:
Symbol Descriptor
BIOHAZARD: Indicates that a biohazard will, could, or might occur.
BURN HAZARD: Alerts you to the presence of a hot surface that could or
might cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.
FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.
EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates
this risk could or might cause physical injury.
FLAMMABLE GAS HAZARD. Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
CLOTHING REQUIRED. Indicates that you should wear a work clothing when
performing a task or else physical injury could or might occur.
BOOTS REQUIRED. Indicates that you must wear boots when performing a
task or else physical injury could or might occur.
MATERIAL AND EYE HAZARD. Indicates you must wear eye protection
when performing a task.
HAND AND CHEMICAL HAZARD: Indicates that chemical damage or
physical injury could or might occur.
HARMFUL. Indicates that the presence of harmful material will, could, or
might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.
xviii TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Preface
3
Instrument Markings and Symbols
LIFTING HAZARD. Indicates that a physical injury could or might occur if
two or more people do not lift an object.
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for
a physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.
RADIOACTIVE HAZARD. Indicates that the presence of radioactive material
could or might occur.
For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard. In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.
Instrument Markings and Symbols
Ta bl e 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on TriPlus 500 HS, which are annotated with an asterisk below.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
* Alternating Current
Both direct and alternating current
Three-phase alternating current
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xix
Preface
Safety Information and Warnings
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
* On (Supply)
* Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
Fuse
* Instruction manual symbol affixed to product. Indicates that the you must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock
* Caution, hot surface
* Caution, biohazard
* Symbol in compliance to the Directive 2012/19/EU on Waste Electrical
and Electronic Equipment (WEEE) placed on the European market after
August, 13, 2005.
Safety Information and Warnings
This safety guide raises awareness of potential safety issues and general points for
consideration for Thermo Fisher Scientific representatives during installation, and repair of
TriPlus 500 HS, or parts of it (following the life cycle principle), as well as for the end user of
TriPlus 500 HS in the lab during the learning phase, and in routine work.
IMPORTANT Read this section first before operating with TriPlus 500 HS.
General Considerations
• Before a unit is put to use, consult the TriPlus 500 HS manuals and related documents
under all circumstances.
• Changes or modifications to this unit not expressly approved by the party responsible for
compliance, could void your authority to operate the equipment.
xx TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Electrical Hazards
Preface
Safety Information and Warnings
• Be aware that if the equipment is used in a manner not specified by the manufacturer, the
protective and safety features of the equipment might be impaired.
• The repair of instrument failures caused by operation in a manner not specified by the
manufacturer is expressly excluded from the standard warranty and service contract
coverage.
• When for technical reasons it is necessary to work on instrument parts which might
involve a potential hazard (heated/moving parts, components under voltage, and so on)
contact the Thermo Fisher Scientific authorized representative.
Routine maintenance operations can be performed by a Thermo Fisher Scientific
representative. Alternatively they can be performed by a trained operator. Routine
maintenance can be performed according to the instructions reported in the TriPlus 500
Headspace Sampler Hardware Manual.
Every analytical instrument has specific hazards. Be sure to read and comply with the
following precautions. They ensure the safety and long-term use of your TriPlus 500 HS.
The installation over-voltage category is Level II. The Level II category pertains to equipment
receiving its electrical power from the local level, such as an electrical wall outlet.
The power line and the connections between TriPlus 500 HS and other instruments, used in
the configuration setup of the total analytical system, must maintain good electrical
grounding. Poor grounding represents a danger for the operator, and might seriously affect the
performance of the instrument.
Do not connect TriPlus 500 HS to power lines that supply devices of a heavy duty nature,
such as motors, refrigerators and other devices that can generate electrical disturbances.
Use only fuses of the type and current rating specified. Do not use repaired fuses, and do not
short-circuit the fuse holder. The supplied power cord must be inserted into a power outlet
with a protective earth (ground) contact. When using an extension cord, make sure that the
cord also has an earth contact.
If the supplied power cord does not fit the local electrical socket and a replacement or adapter
has to be purchased locally, make sure that only a certified power cord is used. Any power cord
used must be certified by the appropriate local authorities.
Do not to leave any cable connecting TriPlus 500 HS and the chromatographic system, or the
power cord close to heated zone, such as the injector or detector heating blocks, or the GC hot
air vents.
Always replace any cable showing signs of damage with another one provided by the
manufacturer. Safety regulations must be respected.
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xxi
Preface
Safety Information and Warnings
Do not change the external or internal grounding connections. Tampering with or
disconnecting these connections could endanger you and damage the TriPlus 500 HS.
The instrument is properly grounded in accordance with these regulations when shipped.
To ensure safe operation, do not make any changes to the electrical connections or the
instrument's chassis.
Do not turn the instrument on if you suspect that it has incurred any type of electrical
damage. Instead, disconnect the power cord and contact a Thermo Fisher Scientific
representative for a product evaluation. Do not attempt to use the instrument until it has been
evaluated. Electrical damage might have occurred if TriPlus 500 HS shows visible signs of
damage, exposure to any liquids or has been transported under severe stress.
Damage can also result if the instrument is stored for prolonged periods under unfavorable
conditions: for example, subjected to heat, moisture, and so on. Ensure that the power
supply/controller unit is always placed in a clean and dry position. Avoid any liquid spills in
the vicinity.
Before attempting any type of maintenance work, always disconnect the power cords from the
power supply if optional devices are installed. Capacitors inside the instrument might still be
charged also if the instrument is turned off.
To avoid damaging electrical parts, do not disconnect an electrical assembly while power is
applied to TriPlus 500 HS. After the power is turned off, wait approximately 30 seconds
before you disconnect an assembly.
Other Hazards
The instrument includes a number of integrated circuits. These circuits might be damaged if
exposed to excessive line voltage fluctuations, power surges or electrostatic charges, or both.
The power supply for TriPlus 500 HS has the symbols I/O on the label for the power switch
to indicate On/Off. It is important that the power On/Off switch is accessible to unplug the
AC power cord from the power supply/wall outlet in case of emergency.
Danger of crushing to fingers and hands. To avoid injury keep your hands away from moving
parts during operation. Turn off the power to TriPlus 500 HS if you must reach inside a
mechanically powered system with moving parts.
To avoid injury, observe safe laboratory practice when handling solvents, changing tubing, or
operating the TriPlus 500 HS. Know the physical and chemical properties of the solvents you
use. See the Safety Data Sheets (SDS) from the manufacturer of the solvents being used.
When using TriPlus 500 HS, follow the generally accepted procedures for quality control and
method development.
Do not operate on the instrument components that form part of the work area of TriPlus 500
HS when it is in motion.
xxii TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Do not use vials without a sealing cap. Vapor phase from organic solvents can be hazardous
and flammable. Acidic vapor phase can cause corrosion to critical mechanical parts.
Use high quality vials and closures as depending on the application conditions, high pressure
can build up in the vial. Do not reuse headspace vials. Repeated heating of reused vials may
increase the chance of vial breaking.
Working with Toxic or other Harmful Compounds
WARNING Before using hazardous substances (toxic, harmful, and so on), please read the
hazard indications and information reported in the applicable Safety Data Sheet (SDS).
Use personal protective equipment according to the safety requirements.
• Protective gloves: Loose fitting thermal insulated or leather gloves.
• Eye protection: Full face shield and safety glasses are recommended.
• Other protective equipment: Safety shoes when handling containers. Long sleeved
shirts and trousers without cuffs. Work clothing that sufficiently prevents skin contact
should be worn.
Preface
Safety Information and Warnings
Biological Hazards
Before using dangerous substances (toxic, harmful, and so on), read the hazard indications
and information reported in the Safety Data Sheet (SDS) supplied by the manufacturer,
referring to the relevant CAS (Chemical Abstract Service) number.
TriPlus 500 HS may require the use of several chemical products with different hazard
characteristics, change which are present in vials. Before using these substances, please read the
hazard indications and information reported in the SDS supplied by the manufacturer
referring to the relevant CAS number.
When preparing the samples, please refer to local regulations for the ventilation conditions of
the work room.
All waste materials must be collected and eliminated in compliance with the local regulations
and directives in the country where the instrument is used.
ATTE NTIO N If using dangerous or flammable solvents, it is suggested to work with a fume
hood placed over the TriPlus 500 HS.
In laboratories where samples with potential biological hazards are handled, you must label
any equipment or parts thereof which might become contaminated with biohazardous
material.
When working with biohazardous materials, it is your responsibility to fulfill the following
mandatory requirements:
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xxiii
Preface
Safety Information and Warnings
Maintenance
• Instructions on how to safely handle biohazardous material must be provided.
• Operators must be trained and made aware of the potential dangers.
• Personal protective equipment must be provided.
• Instructions must be provided on what to do in case operators are exposed to aerosols or
vapors during normal operation (within the intended use of the equipment) or in case of
single fault situations such as a broken vial.
The protective measures must consider potential contact with the skin, mouth, nose
(respiratory organs), and eyes.
• Instructions for decontamination and safe disposal of the relevant parts must be provided.
It is your responsibility to handle hazardous chemicals or biological compounds (including,
but not limited to, bacterial or viral samples and the associated waste), safely and in
accordance with international and local regulations.
Any external cleaning or maintenance must be performed with TriPlus 500 HS turned off and
the power cord disconnected.
Avoid using solvents and spraying on electrical parts. For the removal of potentially dangerous
substances (toxic, harmful, and so on) read the hazard indications and information reported
in the Safety Data Sheet (SDS) supplied by the manufacturer referring to the relevant CAS
(Chemical Abstract Service) number. Use proper protective gloves.
When working with hazardous materials such as radioactive, biologically hazardous material,
and so on, it is important to train all operators how to respond in case of spills or
contamination.
Depending on the class of hazardous material, the appropriate measures have to be taken
immediately. Therefore, the chemicals or solvents needed for decontamination have to be on
hand.
Any parts of the equipment which can potentially be contaminated, such as the sample vial
tray, and so on, must be cleaned regularly. The waste solvent from cleaning and any hardware
which requires to be disposed of has to be properly eliminated with all the necessary
precautions, abiding by national and international regulations.
When preparing for decontamination, ensure that the solvent or chemical to be used will not
damage or react with the surface, dye (color) of the instrument, table or other nearby objects.
If in doubt, please contact your Thermo Fisher Scientific representative to verify the
compatibility of the type or composition of solvents with TriPlus 500 HS.
xxiv TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Disposal
Preface
Writing Convention Used in This Guide
Do not dispose of this equipment or parts thereof unsorted in municipal waste.
Follow local municipal waste regulations for proper disposal provisions to reduce
the environmental impact of waste electrical and electronic equipment (WEEE).
European Union customers: Call your local customer service representative
responsible for TriPlus 500 HS for complimentary equipment pick-up and
recycling.
WARNING The customer has to ensure that TriPlus 500 HS has not been contaminated
by any hazardous chemical or biological compounds including (but not limited to)
bacteria or viruses.
Any part which had direct contact with the analytical sample must be identified and must
undergo an appropriate decontamination procedure prior to shipping for disposal.
Potentially dangerous components are vials and trays. Any critical parts sent for disposal
must be handled according to national laws for hazardous compounds.
The customer and the service engineer are fully responsible for enforcing these
requirements. Thermo Fisher Scientific will hold the representative, customer responsible,
or both, if these regulations are not observed.
Writing Convention Used in This Guide
ATTENTION For writing convenience the TriPlus 500 Headspace Sampler is identified or
as TriPlus 500 HS or simply as HS sampler.
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xxv
1
Getting Familiar with Your TriPlus 500 Headspace Sampler
This chapter provides information to familiarize users with the TriPlus 500 Headspace
Sampler (TriPlus 500 HS or HS sampler).
Contents
• Introduction
• Instrument Basics
• Label Location on the Instrument
• Incubation Group
• Sampling Path
• Pneumatic Connections
• Electrical Connections
• Status Panel
• TRACE 1310 GC User Interface
• Vial Loader
• Sample Trays
• Barcode Reader
• Heated/Cooled Tray
• TriPlus 500 Web Interface
Thermo Scientific TriPlus 500 Headspace Sampler User Guide 1
1
Getting Familiar with Your TriPlus 500 Headspace Sampler
Introduction
Introduction
The TriPlus 500 HS is an
automatic sampler for headspace
gas chromatographic (HSGC)
analysis for the determination of
volatile compounds in a liquid
or solid matrices.
The samples are placed in
headspace vials, tightly closed
with a suitable cap and septum.
Each vial is heated, and the
volatile compounds are
transferred from the solid or
liquid sample into the gaseous
phase above it, called headspace,
until a condition of
thermodynamic equilibrium is
reached.
Afterwards, an aliquot of
headspace is withdrawn and
injected into the gas chromatograph. Details on the principles of operation are available in the
section “Analytical Cycle” on page 34.
Besides a sampling technique, the headspace technique is also an extraction and concentration
technique. It offers the advantages of a reduced manipulation of the sample, a higher
sensitivity than the direct injection, and a longer lifetime of chromatographic columns since
only the volatile fraction is injected into the column.
The TriPlus 500 HS is directly coupled with a TRACE 1300 Series GC and controlled by a
Thermo Scientific Chromatography Data System, through the GC touch screen, if available,
or the virtual GC user interface.
2 TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Instrument Basics
TriPlus 500 HS-12
TRACE 1300 GC
TriPlus 500 HS-12
TRACE 1310 GC
The TriPlus 500 Headspace Sampler (TriPlus 500 HS) consists of a 12-vial capacity
configuration (TriPlus 500 HS-12), upgradeable to 120-vial capacity with the addition of a
vial loader and a tray holder with three removable 40-vial trays (TriPlus 500 HS-120). The
TriPlus 500 HS-120 can be further extended to 240-vial capacity with an additional tray
holder and three 40-vial trays on the top of the GC.
See Figure 1 and Figure 2.
Figure 1. TriPlus 500 HS-12 Coupled with a TRACE 1300 Series GC
1
Getting Familiar with Your TriPlus 500 Headspace Sampler
Instrument Basics
Thermo Scientific TriPlus 500 Headspace Sampler User Guide 3
1
TriPlus 500 HS-120
Sample Vial Trays
TriPlus 500 HS-120
TRACE 1310 GC
TRACE 1300 GC
TriPlus 500 Vial Loader
Getting Familiar with Your TriPlus 500 Headspace Sampler
Instrument Basics
Figure 2. TriPlus 500 HS-120 Coupled with a TRACE 1300 Series GC
4 TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Loading...