Thermo Scientifi? Dionex Chemically Regenerated Suppressor 500 Series, Dionex CRS 500 Series, Dionex ACRS 500, Dionex CCRS 500 Product Manual
Part of Thermo Fisher Scientific
Thermo Scientific
Dionex CRS 500
Product Manual
P/N: 031727-07 March 2015
Thermo Scientific
Product Manual for Dionex CRS 500 Suppressor
Page 2 of 40
Product Manual
for
Dionex Chemically Regenerated Suppressor 500
Dionex ACRS 500, 4 mm, (P/N 085090)
Dionex CCRS 500, 4 mm, (P/N 085092)
Dionex ACRS 500, 2 mm, (P/N 085091)
Dionex CCRS 500, 2 mm, (P/N 085093)
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Thermo Scientific
Product Manual for Dionex CRS 500 Suppressor
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© 2015 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. provides this docu ment to its customers with a product purchase to use in the product
operation. This document is copyright protected and any reproduction of the whole or any part of this document is
ede all previous
Revision History:
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strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this docu ment ar e subj ect to change without notice. All tech nical infor mati on in this d ocu ment is for
reference purposes only. System configurations and specifications in this document supers
information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error free and
assumes no responsibility and will not be liable for any errors, o missions, da mage or loss that might result from any
use of this document, even if the information in the document is followed properly.
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shall govern all conflicting information between the two documents.
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 06, January, 2015 – Rebranded for Thermo Scientific. Product name changed from Dionex MMS 300 to
CRS 500.
Revision 07, March, 2015 – Typo and formatting corrections.
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Product Manual for Dionex CRS 500 Suppressor
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Indicates a potentially hazardous situation which, if not avoided, could result in death or
Indicates a potentially hazardous situation which, if not avoided, may result in minor or
!
!
!
NOTE
!
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
serious injury.
Indicates a potentially hazardous situation which, if not avoided, could result in damage to
equipment.
IMPORTANT
Tip
moderate injury. Also used to identify a situation or practice that may seriously damage the
instrument, but will not cause injury.
Indicates information o f general interest.
Highlights information necessary to prevent damage to software, loss of data, or invalid test
results; or might contain information that is critical for optimal performance of the system.
Highlights helpful inform ation that can make a task easier.
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Contents
Thermo Scientific
Product Manual for Dionex CRS 500 Suppressor
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Contents
1. Introduction ......................................................................................................... 7
1.1 Chemically Regenerated Suppressor .............................................................................................. 7
1.2 Dionex Chemically Regenerated Suppressor Assembly Schematic ............................................... 9
1.3 Chemical Suppression Schematic ................................................................................................. 11
1.4 Shipment and Storage ................................................................................................................... 12
1.4.1 Shipment ............................................................................................................................................. 12
1.4.2 Storage ................................................................................................................................................ 12
1.5 Overview of Suppression Modes .................................................................................................. 13
1.6 Mode of Operation Selection ........................................................................................................ 14
1.6.1 The Displacement Chemical Regeneration (DCR) Mode ................................................................... 15
1.6.2 The External Chemical Mode ............................................................................................................. 16
1.6.3 The AutoRegen Suppres s i on Mode ..................................................................................................... 17
2. Installation .........................................................................................................18
2.1 System Requirements ................................................................................................................... 18
2.2 Back Pressure Coils for the Dionex CRS 500 .............................................................................. 19
2.3 Plumbing for Displacement Chemical Regeneration (DCR) Mode of Operation ........................ 21
2.4 Plumbing for External Chemical Mode of Operation ................................................................... 23
2.5 Peristaltic Pump installation ......................................................................................................... 24
2.6 Plumbing for AutoRegen Suppression Mode of Operation .......................................................... 25
3. Operation ...........................................................................................................27
3.1 Chemical Purity Requirements ..................................................................................................... 27
3.1.1 Inorganic Che mic a l s ............................................................................................................................ 27
3.1.2 Solvents ............................................................................................................................................... 27
3.1.3 Deionized Water.................................................................................................................................. 27
3.2 Start Up ......................................................................................................................................... 28
3.3 Optimizing Regenerant Concentration and Flowrate ................................................................... 29
3.3.1 Anion Regenerant Concentration for Optimized Operation in
External Chemical and AutoRegen Modes ......................................................................................... 29
3.3.2 Cation Regenerant Concentration for Optimized Operation for
External Chemical and AutoRegen Modes ......................................................................................... 30
3.3.3 Calculations for Regenerant Concentration......................................................................................... 32
3.3.4 Regenerant Concentrat ion for DCR Mode .......................................................................................... 33
3.4 Storage .......................................................................................................................................... 33
3.4.1 Short Term Storage (1 to 5 days) ........................................................................................................ 33
3.4.2 Long Term Storage ............................................................................................................................. 33
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Product Manual for Dionex CRS 500 Suppressor
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4. Troubleshooting Guide .....................................................................................34
4.1 Small Analyte Peak Areas ............................................................................................................ 34
4.2 High Background Conductivity .................................................................................................... 34
4.3 Drifting Baseline........................................................................................................................... 35
4.4 Decreased Sensitivity ................................................................................................................... 36
4.5 System Back Pressure Increases Over Time ................................................................................ 36
4.6 Liquid Leaks ................................................................................................................................. 37
5. Suppressor Cleanup ..........................................................................................38
5.1 Base-Soluble Contaminants or Precipitates with the Dionex CRS 500 ........................................ 39
5.2 Organic Contaminants with the Dionex ACRS 500 or Dionex CCRS 500 .................................. 40
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1 – Introduction
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Product Manual for Dionex CRS 500 Suppressor
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1. Introduction
Suppressor: The role of a suppressor in Ion Chromatography is to remove the eluent and sample
counterions and replace this with regenerant ions thereby converting the eluent to a weakly
dissociated form prior to detection. Detection of analyte ions particularly with conductivity
detection is therefore feasible against a low background. The suppressor not only reduces the
background signal but also the noise associated with the signal. Furthermore, the analytes are
converted to the more conductive acid or base form, which enhances the signal, particularly for
fully dissociated species. Thus overall i mprovement in detection limits as observed from the
signal to noise ratio is achieved. When compared to single column ion chromatography, i.e.,
applications that do not use a suppressor, the improvement in noise with suppressed ion
chromatography far exceeds the noise performance of single column chromatography
applications. Hence the suppressor has become an integral part of the ion chromatography
instrument.
The suppressors from Thermo Fisher Scientific are designed for continuous operation and do
not require any switchin g or offline r egeneratio n. Furthermore , the standar ds and the s amples
are always exposed to the same suppressor device when pursuing ion analysi s, thus ensuring
that the analytical parameters are consistent between calibration and analysis. From a simplistic
perspective there are two types of suppressors offered for continuous operation, namely,
electrolytically regenerated suppressors and chemically regenerated suppressors. The
electrolytic suppressors operate continuously with a water source as a regenerant. In the recycle
mode of operation the water source is derived from the suppressed eluent, thereby making the
suppressor operation facile. The chemical suppressors operate continuously with an external
regenerant source.
The electrolytic suppressor also is a device that permits recycle of the eluent when installed in a
system with Eluent Recycle (ER) s ys t em.
1.1 Chemically Regenerated Suppressor
The T hermo Scientific™ Dionex™ Chemically Regenerated Suppressor (Dionex CRS™ 500)
replaces the Dionex MicroMembrane Suppressor (MMS 300 ) product line. The Dionex CRS
500 is a chemical suppressor with a new hardware design that allows the suppressor to be more
pressure tolerant than previous generation suppressor devices. The suppressor flow pathway has
been redesigned to optimize band dispersion, improve the flow and sealing properties. The
Dionex CRS 500 continues to use the same cleaned ion exchange components (screens and
membranes) as the Dionex MMS 300 suppressor devices; however the Dionex CRS 500 eluent
channel uses an ion exchange resin bed as opposed to a gasketed screen.
The Chemically Regenerated Suppressor (CRS 500) is available in two versions: the Anion
Chemically Regenerated Suppressor (Dionex ACRS™ 500) or Cation Chemically Regenerated
Suppressor (Dionex CCRS™ 500) to support anion and cation analysis applications.
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Product Manual for Dionex CRS 500 Suppressor
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Regenerant is pumped in
Regenerant is Diverted to Waste
or
Recycled to AutoRegen Module
Eluent In
From Analytical Column
Eluent Out
To Detector Cell
with Back Pressure Coils
and then to Waste
or
to DCR Reservoir
Figure 1 The Chemical Suppression System
The Dionex Chemically Regenerated Suppressors, Dionex Anion Chemically Regenerated
Suppressor (Dionex ACRS 500) and the Dionex Cation Chemically Regenerated Suppressor
(Dionex CCRS 500), provide a high performance, highly reliable, low maintenance chemical
suppression system for Ion Chromatography.
The chemical suppression system consists of the Dionex CRS 500 and a source of chemical
regenerant. Through an ion exchange based neutralization reaction in the Dionex CRS 500
Suppressor device, the eluent is converted to a weakly dissociated lower conductivity form
while the analytes are converted typically to a more conductive form.
The Dionex CRS 500 is a non-electrolytic device and therefore requires no power supply.
Installation of equipment (either the Displacement Chemical Regeneration (DCR) kit, a
pressurized bottle delivery s ystem, additional pump or the AutoRegen Accessory) is needed in
conjunction with the Dionex CRS 500. This equipment facilitates the delivery of the regenerant
to the Dionex CRS 500 suppressor. A regenerant controller Thermo Scientific™ Dionex
TM
SRD-10 Suppressor Regenerant Detector is reco mmended to monitor the chemical regenerant
flow and trigger when to replace the chemical regenerant. If the regenerant flow is restricted or
stops the SRD-10 auto matically disables the eluent pump.
The Dionex CRS 500 provides high capacity suppression while adding minimum dead volume
to the analytical system. The ability of the Dionex ACRS 500 to provide continuous suppression
of traditional eluents, and more concentrated eluents up to 100 mM NaOH, significantly
expands the capabilities and simplifies the operation of anion exchange Ion Chromatography.
The ability of the Dionex CCRS 500 to provide continuous suppression of traditional eluents,
and more concentrated eluents up to 75 mM HCl, significantly expands the capabilities and
simplifies the operation of cation exchange Ion Chromatography.
The Dionex ACRS 500 and Dionex CCRS 500 are available in both 4 mm and 2 mm formats
for use with 4 mm and 2 mm Ion Chromatography columns and systems respectively. The 4 mm
Dionex CRS 500 is designed to ensure optimum performance with 4 and 5 mm columns. The 2
mm Dionex CRS 500 is designed with reduced internal volume to ensure optimum performance
with, 2 and 3 mm columns and systems.
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Product Manual for Dionex CRS 500 Suppressor
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NOTE
!
For assistance, contact Technical Support for Dionex Products. In the U.S., call 1-800346-6390. Outside the U.S., call the nearest Thermo Fisher Scientific office.
1.2 Dionex Chemicall y Regen erat ed Suppressor Assembly Schematic
The Dionex CRS 500 includes two regenerant compartments and one eluent compartment
separated by ion exchange membranes. The central channel is the eluent channel and the two
side channels are regenerant channels. Two PEEK plates form the outer walls of the regenerant
channels and have t he ¼-28 port s for co nnecti ng the r egene rant li quid li nes. T he elue nt cha nnel
is defined by a PEEK plate that seals against the ion exchange membrane and a thin elastomeric
o-ring installed on the regenerant PEEK plates. The eluent in and out ports are independent
ports that define the fluidic pa thwa y, which i s simi lar to a co lumn The regenerant flo w dir ectio n
is opposite to the elue nt flow direction. This orie ntation ensures complete regeneratio n of the
device.
The regenerant channels are flushed with a regenerant tha t supplies hydronium or hydroxide
ions that are required for the suppression reaction. For the Dionex ACRS 500, the ion e xc han ge
membranes provide the transport pathwa y for the hydronium ions into the eluent channel while
providing a transport pathway for the sodium or potassium ions out o f the eluent cha nnel. For
the Dionex CCRS 500, the ion exchange membranes provide the transport pathway for the
hydroxide ions into the eluent channel while providing a transport pathway for the sulfate or
MSA ions out of the eluent channel. In the Dionex CRS 500 the rege nerant channel is fitted
with unfunctionalized neutral regenerant screens that facilitate excellent transport of ions to and
from the ion exchange membranes without any retention in the regenerant channel. The net
result for both the Dionex ACRS 500 and t he Dionex CCRS 500 is suppression o r conversion of
the eluent from a hi ghly conduc tive for m to a wea kly cond uctive for m and conversion of most
analytes into highly conductive forms.
For example, for the Dionex ACRS 500, sodium hydroxide eluent with residual carbonate is
converted to water and a weakly conductive carbonic acid background, while sodium chloride
analyte is converted to a highly conductive hydrochloric acid form. Thus the analyte, chloride, is
detected as the highly conductive HCl form against a low conductivity water and residual
carbonic acid background. For the Dionex CCRS 500, methanesulfonic acid eluent is converted
to water and the analyte, sodium chloride, is converted to sodium hydroxide. Thus sodium is
detected as the highly conductive NaOH form against a low conductivity water bac kground. It
should be noted that the background of the suppressed eluent may be elevated due to chemical
leakage of the regenerant into the eluent; therefore optimization of the regenerant type,
concentration and flow rate may be needed to minimize this effect.
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Product Manual for Dionex CRS 500 Suppressor
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Regen In
Regen Out
Eluent In
Eluent Out
Figure 2 Membrane and Flow Chamber Configuration in the Dionex Chemically
Regenerated Suppressors
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Product Manual for Dionex CRS 500 Suppressor
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1.3 Chemical Suppression S chematic
Chemical Suppression with the Dionex ACRS 500 is a neutralization reaction and selective
desalting process carried out across a pair of cation exchange membranes. The cation exchange
membranes facilitate the selective exchange of cations in the suppressor. With sodium
hydroxide as the eluent, sodium ions are continuously exchanged for hydronium ions from the
regenerant acid and combine with the eluent hydroxide to form water. The sodium ions are
removed continuously from the suppressor by the regenerant acid stream thus effecting
continuous suppression.
Chemical Suppression with the Dionex CCRS 500 is a neutralization reaction and selective
desalting process carried out across a pair of anion exchange membrane s.. The a nion excha nge
membranes facilitate the selective exchange of anio ns in the supp ressor. Wi th methanes ulfonic
acid as the eluent, the methanesulfonate ions are continuously exchanged for hydroxide ions
from the regenerant base (TBAOH) and combine with the eluent hydronium to form water. The
methanesulfonate anions are removed continuously from the suppressor by the regenerant base
stream thus effecting continuous suppression.
Figure 3 Figure 4
Chemical Suppression with the Chemical Suppression with the
Dionex Anion Chemically Regenerated Dionex Cation Chemically Regenerated
Suppressor (Dionex ACRS 500) Suppressor (Dionex CCRS 500)
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Product Manual for Dionex CRS 500 Suppressor
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storage or
Ensure the suppressor is stored in a temperature controlled environment away from direct
!
!
1.4 Shipment and Storage
1.4.1 Shipment
The Dionex Chemically Regenerated Sup pressors (Dionex ACRS 500 and Dionex CCRS
500) should not be subjected to temperatures above 60°C during shipment,
operation for extended periods of time.
1.4.2 Storage
exposure to sunlight or other sources of heat. Do not store the suppressor in a nontemperature controlled environment where temperatures in excess of 40°C are commonly
experienced, such as a parked car, a tool shed, or a lab-bench in close proximity to an open
window with direct sunlight.
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