Thermo Scientifi? Cryostat Microm Cryo-Star HM 560 Instruction Manual

Thermo Scientific Cryostat Microm Cryo Star HM 560

Thermo Scientific
Microtome Cryostat
Microm Cryo-Star HM 560

INSTRUCTION MANUAL

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

1

Thermo Scientific Cryostat Microm Cryo Star HM 560

2

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

CERTIFICATION

Thermo Fisher Scientific Microm International GmbH certifies that this instrument
has been tested and checked carefully. Its technical data was verified before
shipment to be in accordance with the published specifications.

The instrument complies with applicable international safety regulations.

WARRANTY

This Thermo Fisher Scientific product is warranted against defects in material and
workmanship for a period of 1 year. Parts which prove to be defective during the
warranty period will be repaired or replaced free of charge by the manufacturer. No
other warranty is expressed or implied. Unauthorized modification or repair by third
party persons will void the warranty.
The warranty will expire in case of improper or wrong use of the instrument and in
case the warning and precautionary messages are not observed. Thermo Fisher
Scientific is not liable for any occurring damage.

Errors and omissions excepted. Subject to amendment and improvement without
further notice.

This instruction manual will be supplied together with each instrument. Further
copies can be ordered at the nearest Thermo Fisher Scientific sales office by giving
the serial number of the instrument, the number of the instruction manual and the
date of issue.

This instruction manual is available in the following languages:

Cat. No.
German: 387 490
English: 387 500
French: 387 510

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

3

Thermo Scientific Cryostat Microm Cryo Star HM 560

INTENDED USE

Dear valued Customer,

Thank you for buying this Thermo Fisher Scientific instrument.

Before putting the instrument into operation, please read these operating
instructions carefully to familiarize you with its proper operation and
functions.

Only skilled or specially trained personnel must operate the microtome
cryostat HM 560, i.e. clamping the specimen, trimming, sectioning and taking
off the sections from the instrument. The listed and marked safety measures
as well as the regulations and hygiene measures of your respective lab must
strictly be observed.

Serial No.: __________________

Please check the Serial No. on the type plate, which is placed on the rear
side of your instrument and enter this number here. This way, questions and
service can be handled faster.

Instruction Manual No. 387500

Issued on February 03rd, 2009

Microm International GmbH
part of Thermo Fisher Scientific
Robert-Bosch-Strasse 49

69190 Walldorf
Germany

Phone: [phone]
Fax: [phone]
Email: [e-mail]
Internet: www.ap-walldorf.com

4

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

Intended Use

Table of Contents

Safety Precautions

PART 1 INTRODUCTION

1-1 Description of the Cryo-Star HM 560
1-2 Technical data Cryo-Star HM 560

PART 2 OPERATING INSTRUCTIONS

2-1 Setting up the cryostat

2-2 Initial turn-on

2-3 Basic operational rules

2-4 Setting section thickness and trimming thickness

2-5 Specimen and knife cooling
2-5-1 Actual and set value of the specimen temperature
2-5-2 Actual and set value of the knife temperature
2-5-3 Function fast freezing
2-5-4 Programming temperature parameters

2-6 Cutting movement and retraction

2-7 Feed
2-7-1 Knife carrier coarse feed
2-7-2 Speed of the knife carrier coarse feed
2-7-3 Automatic approach system
2-7-4 Trimming and first cuts
2-7-5 Fine feed

2-8 Setting the cutting window

2-9 Hand wheel brake

2-10 Detaching the specimen

2-11 Indication of cutting processes
2-11-1 Section counter
2-11-2 Section thickness sum
2-11-3 Remaining travel to front end position
2-11-4 Real time

2-12 Setting the real time, WAKE time and ACTIVE time
2-12-1 Setting the real time
2-12-2 Setting the WAKE time
2-12-3 Setting the ACTIVE time

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

5

Thermo Scientific Cryostat Microm Cryo Star HM 560

2-13 Defrosting
2-13-1 Setting the defrost time
2-13-2 Defrosting cycle
2-13-3 Interrupting a defrosting cycle

2-14 Turning off the function retraction

2-15 Customer-specific settings
2-15-1 Setting the contrast
2-15-2 Selecting the language

2-16 Turning off the cooling function

2-17 Illumination of the cooling chamber

2-18 Adjusting the operating panel

2-19 Specimen holder, freezing-on and specimen orientation
2-19-1 Specimen holder, freezing-on
2-19-2 Specimen orientation

2-20 Knife carriers
2-20-1 Standard knife carrier SM
2-20-2 Disposable blade holder SE
2-20-3 Modification from standard knife carrier SM to disposable blade holder SE

2-21 Error code indication
2-21-1 Definition of the error codes

2-22 Accessories
2-22-1 Standard equipment
2-22-2 Additional equipment

PART 3 Theory of operation

3-1 Specimen and knife carrier cooling, defrost cycle

3-2 Cutting movement

3-3 Knife carrier coarse feed and trimming stages

3-4 Automatic approach system

PART 4 Working with the cryostat

4-1 Preparations on the microtome and inside the cooling chamber

4-2 Freezing on the specimen

4-4 Orientation and trimming of specimens

4-5 Sectioning and taking off sections

4-6 How to avoid malfunctions

6

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

PART 5 Maintenance of the cryostat

5-1 Shutting-off for cleaning

5-2 Cleaning and care of the microtome

5-3 Changing the fluorescent lamp

5-4 Disposal of the instrument after final shutdown

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

7

Thermo Scientific Cryostat Microm Cryo Star HM 560

SAFETY PRECAUTIONS

WARNING SIGNALS AND SYMBOLS

The installation and routine use of the Cryo-Star HM 560 is easy and safe if the instructions in this manual are
being observed.
However, the situations which might be risky for the personnel or equipment are specially marked in this
manual with the following symbols and messages.

Note:

Special instructions regarding operation of the instrument.

Caution:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.

Danger:

Special warning messages to prevent harm to persons and/or serious damage to equipment. For
your own safety, please observe these instructions carefully.

Biohazard:

Warning of biological danger.

Separate taking back of electrical and electronic instruments in the countries of the
European Union:

This is to be applied in the countries of the European Union and other European countries with a
separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).

8

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

SAFETY PRECAUTIONS

A T T E N T I O N !

The operator's safety is affected, when the instrument is not operated in
accordance with this instruction manual.
Please observe the following general precautions during operation of this
instrument. Failure to comply with these precautions violates safety standards and
the intended use of the instrument. Thermo Fisher Scientific is not liable for misuse
of the instruments and failure to comply with basic safety requirements.

INSTRUMENT GROUNDING

To avoid injury from electrical current, the instrument must be connected with the safety ground. The
instrument is equipped with a three wire ground plug. The power outlet must be connected to the safety
ground and must meet the International Electrotechnical Commission (IEC) regulations.

CAUTION: MAINS VOLTAGE

Never remove instrument covers during operation. Component replacements as well as adjustments must
only be made by trained service personnel. Unplug the unit before removing or opening the covers.

DANGER IN EXPLOSIVE ENVIRONMENT

The instrument must not be operated in the presence of flammable gases.

HAZARD OF FROSTBITE

Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at
unprotected hands and arms.

HAZARD OF RADIOACTIVE RADIATION

When working with radioactive specimens observe all applicable radiation safety procedures. When working
with radioactive contaminated material, appropriate safety and disinfection measures must be carried out.
According to the rules and regulations concerning the handling of radioactive contaminated material of the
respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

9

HAZARD OF INFECTION

Thermo Scientific Cryostat Microm Cryo Star HM 560

Use the appropriate safety and disinfection measures when working with infectious specimens.
According to the rules and regulations concerning the handling of infectious/radioactive contaminated
material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must
be worn.

HAZARD OF BIOLOGICAL DANGER

Specimens used during the intended operation of the instrument might potentially be infectious. For this
reason, it is recommended to observe the general laboratory regulations concerning protection against
danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be read in the
Laboratory Biosafety Manual : 1984 of the World Health Organization.

CAUTION: DANGER OF SQUEEZING

When placing the microtome cryostat HM 560 onto the height adjustable stage, it is absolutely necessary that
the stage is in its upper position or in case of a mechanical stage that the rear wall will be removed. Not
observing this leads to the risk of squeezing your hands between the rear carrying handles and the stage
housing.

HAZARD OF MALFUNCTION

To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic
environment. This means, that transmitters such as mobile phones must not be operated in their close
vicinity.

CARE IN USING MICROTOME KNIFE

To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting
specimen and knife. If possible, the specimen should be clamped in before the knife is inserted
into the knife holder. Before changing the knife holder, always remove blade or knife! Unused

knives should always be kept in a knife case. Never place the knife with the cutting edge upwards.
Never try to catch a dropping knife!! Never check the sharpness of the cutting edge with your fingers. The
cutting edge is extremely sharp!

WASTE DISPOSAL

All debris, waste as well as infectious and radioactive contaminated material from operation must be
disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning liquids as well
as section waste must be disposed of according to the respective regulations for special waste!

10

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

Fig. 1

Microm International GmbH

part of Thermo Fisher Scientific

Robert-Bosch-Str. 49

69190 Walldorf / Germany

387 500 - English

11

Thermo Scientific Cryostat Microm Cryo Star HM 560

1 INTRODUCTION

1-1 DESCRIPTION OF THE
CRYO-STAR HM 560

Open top cryostat. Modular design. Stainless
steel cooling chamber. Temperature regulation
variable down to -50°C for specimen holder
and down to -35°C for knife carrier. Graphic
LC-display of preset and actual temperatures,
electronic control with user-oriented touchpad
keyboard, battery-buffered memory and self­diagnostic system. Standby and sleep status
for noise reduction and energy savings.
Automatic evaporator defrosting and in addition
a manual defrost cycle which can be activated
when needed. Defrost interrupt on keystroke
request. Integrated rapid freezing device with
controlled cooling element down to -60°C.
Freezing device temperature independent of
cryo-chamber temperature. Detach function to
remove used specimen from specimen chucks.
Sliding window with heater and integrated,
position adjustable fluorescent lamp for cryo­chamber. Storage space inside the cryo­chamber. Flat storage space on top of the
housing.

HM-Rotary Microtome with backlash and
maintenance-free cross roller bearings in
stainless steel. Electro-motorized feed system.
Section thickness setting from 0,5 to 100
microns;
up to 2 µm in 0,5 µm-increments,
up to 10 µm in 1 µm-increments,
up to 20 µm in 2 µm-increments,
up to 50 µm in 5 µm-increments and
up to 100 µm in 10 µm-increments.

Trimming thickness setting from 5 to 500
microns;
up to 10 µm in 5 µm-increments,
up to 100 µm in 10 µm-increments,
up to 200 µm in 20 µm-increments and
up to 500 µm in 50 µm-increments.

Specimen retraction during the return stroke
with optical indication. Horizontal feed range 48
mm. Vertical specimen stroke 60 mm. Max.
specimen size 75 x 55 mm.
Motorized coarse feed in two directions with
three speed selections. Limit indication and
automated switch off at front and rear limits of
horizontal travel. Automatic approach system
for exact and safe approach of specimen
towards the knife edge.

Section counter and indication of sum of
section thicknesses with reset-button.
Indication of remaining travel. Hand wheel
brake in any position. Fine orientation of
specimen on two axes. Rotatable on Z-axis,
360°.

Basic outfit with three specimen chucks, cryo­compound, 100 ml cryostat oil, brush shelf and
section waste tray.

A multi-use knife carrier is available. The
standard knife carrier is designed so the knives
as well as the disposable blade holder can be
easily clamped in place and adjusted.
Depending on the version, the disposable
blade holders SE take up low or high profile
blades. The corresponding anti-roll guides
facilitate taking off sections.

12

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

1-2 TECHNICAL DATA CRYO-STAR HM 560

Specimen temperature control: .......................................................................... +10°C to -50°C

Knife carrier control: ................................................................................ -10°C to -35°C

Fast freezing station: ................................................................................. down to -60°C

Defrosting: automatic defrosting, adjustable with temperature control
manual immediate defrosting

Microtome: Section thickness range: ............................................. 1 - 500 µm

Fine section thickness range: .................................... 0,5 - 100 µm

Resolution: .................................................. 0,5 µm for 0,5 - 2 µm

.......................................................................... 1 µm for 2 - 10 µm

........................................................................ 2 µm for 10 - 20 µm

........................................................................ 5 µm for 20 - 50 µm

.................................................................... 10 µm for 50 - 100 µm

Trimming thickness range: ........................................... 5 - 500 µm

Resolution: ........................................................ 5 µm for 5 - 10 µm

.................................................................... 10 µm for 10 - 100 µm

.................................................................. 20 µm for 100 - 200 µm

.................................................................. 50 µm for 200 - 500 µm

Knife carrier retraction during return travel ...........................40 µm

Horizontal knife carrier range ............................................ 48 mm

Vertical specimen stroke ................................................... 60 mm

Read-outs: Graphical LC display: section thicknesses, section counter, sum of
section thicknesses, remaining travel to front end position, size of
the cutting window

Specimen approach: .......................................................................... automatic function

........................................ alternatively manual with variable speed

Temperature parameter: ............................... temperature set values can be stored in pairs

Size of chucks: .......................................................................................30, 40 mm

............................................................. special sizes upon request

Specimen orientation: x - and y - axes: ......................................................... universal 8°

z - axis: ......................................................................... up to 360°

Coarse feed: ........................................... motorized, graduated and continuous

Cooling chamber illumination: ................................................ with variable illumination position

Sliding window: ............................................................................................. heated

Cutting speed: .................................................................................. 0 - 250 mm/s

Hand wheel brake: ................................................................................. in any position

All temperatures refer to an ambient temperature of +20°C!

Cont'd on page -2-

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

13

Thermo Scientific Cryostat Microm Cryo Star HM 560

1-2 TECHNICAL DATA CRYO-STAR HM 560

Storage

temperature range: ......................................................................... -20°C up to +50°C

Operating conditions: ....................... +5°C up to +35°C (at a max. rel. humidity of 60%)

........................................................... altitude up to 2000 m M.S.L.

.......................................................................... for indoor use only

Power requirements: ....................................................... 100 V 12 A +/-10% 50...60 Hz

............................................................... 115 V 12 A +/-10% 60 Hz

................................................. 220...230 V 6 A +/-10% 50...60 Hz

................................................................. 240 V 6 A +/-10% 50 Hz

Pollution degree: ...................................................................................................... 2

Overvoltage category: ...................................................................................................... II

Acoustic pressure: ......................................................................................... 45 dB(A)

............................... measured with 1 m distance to the instrument

Dimensions: .. Wide (w/o Hand wheel): 769 mm, deep: 932 mm, high: 578 mm

Weight: ............................................................................................. 141 kg

All temperatures refer to an ambient temperature of +20°C!

page -2-

14

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

1 = On indication (sleep status)
2 = Button specimen temperature
3 = Button knife temperature
4 = Button TRIM/FEED
5 = Button UP
6 = Button DOWN
7 = LC display
8 = Button fast freezing

Fig. 2
9 = Button options
10 = Button detaching
11 = Scroll button
12 = Button RESET
13 = Button PROG
14 = Info line
15 = Status line

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

15

Thermo Scientific Cryostat Microm Cryo Star HM 560

1 = Button cutting window
2 = Button ACA
3 = Button coarse feed, forwards
4 = Button coarse feed, backwards
5 = Button TRIM

16

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

Fig. 3

Thermo Scientific Cryostat Microm Cryo Star HM 560

1 = Symbol Hand wheel
2 = Button to activate the Hand wheel brake
3 = Button to loosen the Hand wheel brake
4 = Brake LED

Fig. 4

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

17

Thermo Scientific Cryostat Microm Cryo Star HM 560

2 OPERATING INSTRUCTIONS

2-1 SETTING UP THE CRYOSTAT

Note:

If the microtome cryostat is supplied
with an extra height-adjustable stage,
first unpack and set up this stage

according to the separate instruction manual.

Unpacking the instrument:

• Loosen and remove the four tightening

straps.

• Lift and remove the wooden top of the

packing.

• Remove the foam parts upwards.

• The four side parts of the packing, which are

connected with each other, can now be
removed upwards.

• Slightly tilt the cryostat to the side and

remove the right and left lower foam parts
one after the other.

• Lift the cryostat by the carrying handles (fig.

5.1 and 5.2) from the pallet.

• In case the handles are not installed, securely

install the attached carrying handles (fig. 5.1
and 5.2) at the front and rear side of the
instrument and carry the instrument to the
site of installation (table according to the
customer's choice) or place it onto the height­adjustable stage.

Danger:

When placing the microtome cryostat
HM 560 onto the height adjustable

stage, it is absolutely necessary that
the stage is in its upper position.
Not observing this leads to the risk of
squeezing your hands between the rear
carrying handles and the stage housing.

• Remove the handles (fig. 5.1 and 5.2).

cryostat, as when lowering the height­adjustable stage, the handles will hit the stage
and cause damage to the stage.

Caution:

Before the initial set-up of the height-

adjustable stage, it is absolutely

necessary to dismount the two rear

carrying handles (fig. 5.2) on the

Fig. 5

18

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

• Adjust the handles of the cryostat (fig. 5a.1

and 5a.2) on the rear side of the cryostat
stage.

• Carefully keep the shipping container for

possible return shipments.

Choose installation site that

• enough ventilation for the cooling system is

guaranteed.

• the distance between wall and rear panel is

approx. 10 - 15 cm.

• the suction areas on either side are kept free.

Moreover, the installation site must be free
from:

• draught by open doors or by air conditioning

systems.

• direct exposure to sunlight into the cooling

chamber.

Note:

Both measures reduce the formation of

frost and therefore result in more

favourable work conditions. A high air
moisture as well as high ambient temperatures
reduce the maximum performance of the
instrument.

In case the instrument is equipped with the
height-adjustable stage, turn the screws (fig.

5.5) on the front foot ends to fix the complete
unit.

Afterwards:

• Install the attached Hand wheel handle (fig.

5.3).

1

2

Fig. 5a

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

19

Thermo Scientific Cryostat Microm Cryo Star HM 560

2-2 INITIAL TURN-ON

As already mentioned in part 2-1, the height­adjustable stage, if equipped, must be set up
according to the separate instruction manual
before the initial turn-on of the microtome
cryostat.

Caution:

Before turning on the instrument for the

first time, please check if the power

requirements indicated on the type
plate (fig. 7) correspond to the power supply
voltage being used.

• No other instruments should be connected to

the circuit used for the cryostat, as the
compressor needs high surge currents when
started.

• Do not use multi-socket power outlets and

extension lines for the supply of the stage.

• Connect the power line of the instrument to

the power outlet.

• Turn on the power switch (fig. 6.1) on the rear

side of the instrument.

• Close the heated sliding window (fig. 5.4) and

cool down the instrument.

Note:

To avoid frost built-up on the

specimen, the specimen temperature

is controlled to a temperature of +10°C
until the knife has reached a temperature
of -10°C.

The cooling phase will take approx. 1 to 2 h
depending on the set knife temperature
between -15°C and -30°C.

1 = Power switch
2 = Mains plug
5 = Socket for option Vacutome/Disinfection

MICROM

69190 Walldorf, Germany

GmbH

Type HM 560V

Ser. No.XXXXX

Cat. No. 956320

220...230V 6A 50...60Hz

Cooling System

Refr.: R 404a

Amount: 0,275 kg
Max. Press.: 2300 kPa

Made in Germany

I V D

I

0

GmbH

MICROM

69190 Walldorf, Germany

Type HM 560 MV

Ser. No.____

Cat. No. ______

__A / ___ V + 10% __Hz

Fuse 2 x __AT

HP Circuit

Refr.: R 134a
Amount: 0,180kg
Max. Press.: 2300kPa

LP Circuit

Refr.: R 404a
Amount: 0,270kg
Max. Press.: 2300kPa

Made in Germany

1

4

GmbH

MICROM

69190 Walldorf, Germany

Type Stage f. HM 560 HV

Ser. No. __ __
Cat. No. ______

_A /___V +10% __Hz

Fuse 2x __AT

Made in Germany

3

Fig. 6

Fig. 7

20

~___V
F1/F2
2x __AT

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

I

0

Fig. 8

Thermo Scientific Cryostat Microm Cryo Star HM 560

2-3 BASIC OPERATIONAL RULES

When turning on the instrument, the
display shows:

• the configuration of the instrument

• the section thickness selection

• the specimen and knife temperatures with the

corresponding set and actual values

• the symbol for the cutting window

This is the display (fig. 9) while the instrument
is in its active status.

To save energy and extend the lifetime of
some instrument components, the instrument
has two more operative status:

• Standby status

• Sleep status

The instrument switches to the standby status
when it is turned on, but not operated for more
than a pre-selected active time (see part 2-12-

3) between 1 and 9 h. The chamber
illumination goes off and the display (fig. 10)
switches to a large indication of the knife actual
temperature. This is the clear sign for the
standby status and gives the operator
information on the knife temperature, also from
a greater distance.

When the instrument is not operated for
another hour, the cryostat switches to the
sleep status. The display illumination goes off.
However, the equipment-on indicator (fig. 2.1)
then lights up and shows that the instrument is
still on.

Note:

While in the sleep status, the

temperature control of the knife

temperature features a reduced control
accuracy.
The specimen temperature in the sleep status
is always kept at -15°C. The set temperature of
the knife carrier is kept constant.
This does not only save energy, but also
reduces the formation of frost on the specimen
clamping.

Fig. 9

Fig. 10

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

21

Thermo Scientific Cryostat Microm Cryo Star HM 560

• To return to the active operating state, press

any key on the control panels, move the
Hand wheel or wait until the WAKE time (see
2-12-2) has been reached.

The WAKE time is used to trigger the
instrument back into the active state.

UP/DOWN buttons

These two buttons (fig. 11.5 and 11.6) are
used to change pre-selected values of different
kinds in connection with the chosen function.

• section thickness

• trimming thickness

• specimen temperature

• knife temperature

• time settings

Note:

To operate the instrument easily and

comfortably with a few buttons only,

please note the following:

After having chosen a function, its pre-selected
values can be changed via the UP or DOWN
buttons.
However, if no entry is made within 2 seconds,
the operating control switches back into its
basic status.

RESET-button

This button (fig. 12.4) is used to reset added
values (e.g. number of sections, sum of section
thicknesses) to zero. In some cases, this
button is used as additional button for double­button functions (see part 2-8 and
2-13-3).

Keyboard lock

To lock the keyboards and to block the feed
movement, press the buttons (fig. 12.4 and

12.1) for approx. 2 sec. The display (fig. 12a)
shows LOCKED.
To unlock the keyboards press the buttons (fig.

12.4 and 12.1) again.

Fig. 11

Fig. 12

Fig. 12a

Note:

When the keyboards have been

locked, this leads automatically
after 5 sec. to the standby status and after
another 5 sec. to the sleep status.

22

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

Thermo Scientific Cryostat Microm Cryo Star HM 560

2-4 SETTING SECTION THICKNESS
AND TRIMMING THICKNESS

Basic status of the operating control:
To choose between section thickness and
trimming thickness

• press button (fig. 14.4).

• The selected setting is shown inverted on the

display.

• Then enter the desired section or trimming

thickness via button UP (fig. 14.5) or DOWN
(fig. 14.6).

• The changed values are shown on the

display.

The graduation of the section thicknesses
(which can be pre-selected) is divided into 5
ranges:

range graduation

from 0,5 µm to 2 µm 0,5 µm
from 2 µm to 10 µm 1 µm
from 10 µm to 20 µm 2 µm
from 20 µm to 50 µm 5 µm
from 50 µm to 100 µm 10 µm

The graduation of the trimming thicknesses
(which can be pre-selected) is divided into 4
ranges:

range graduation

from 5 µm to 10 µm 5 µm
from 10 µm to 100 µm 10 µm
from 100 µm to 200 µm 20 µm
from 200 µm to 500 µm 50 µm

Fig. 13

Fig. 14

MICROM International GmbH

Robert-Bosch-Str. 49

D- 69190 Walldorf

387 500 - English

23