Thermo Fisher Scientific TrueMark Information Manual

PRODUCT INFORMATION SHEET

TrueMark™ Respir

atory Panel 2.0, OpenArray™ Plate

Catalog Number A49044

Pub. No. MAN0019510 Rev. A.0

Note: For safety and biohazard guidelines, see the “Safety” appendix in the Respiratory Tract Microbiota Proﬁling Experiments v2 using OpenArray™ Application Guide (Pub. No. MAN0019506). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear

appropriate protective eyewear, clothing, and gloves.

For resources that contain detailed information about using the TrueMark™ Respiratory Panel 2.0, OpenArray™ Plate, see “Related documentation” on page 2.

Product description

TrueMark™ Respiratory Panel 2.0, OpenArray™ Plate (Cat. No. A49044) is an ecient, easy-to-use ﬁxed-content OpenArray™ plate for the characterization of key respiratory tract microbial targets. The plate includes 35 TaqMan™ assays that have been optimized for detection of 32 respiratory tract viral and bacterial nucleic acid, including two assays that target SARS-CoV-2. The plate also includes control assays for TaqMan™ Universal Extraction Control Organism (B. atrophaeus), TaqMan™ Universal RNA Spike In/Reverse Transcription (Xeno) Control, and the human RNase P RPPH1 gene. For a complete list of assays included in the plate, see “TaqMan™ assays included in the TrueMark™ Respiratory Panel 2.0, OpenArray™ Plate” on page 1.

The assays perform well with total nucleic acid that is isolated from nasopharyngeal swab, nasopharyngeal aspirate, and bronchoalveolar lavage (BAL) research samples using the MagMAX™ Viral/Pathogen Ultra Nucleic Acid Isolation Kit.

TaqMan™ assay designs and assay target sequences have undergone rigorous bioinformatics selection and analysis to maximize strain coverage and minimize potential for o‐target cross-reactivity. Qualiﬁed TaqMan™ assays have undergone performance testing to ensure that results are accurate with high levels of sensitivity and speciﬁcity.

Contents and storage

Table 1 TrueMark™ Respiratory Panel 2.0, OpenArray™ Plate (Cat. No. A49044; format 112 assays and up to 24 samples)

Component Amount Storage

TrueMark™ Respir

atory Panel 2.0, OpenArray™ Plate 1 plate –25°C to –15°C

TaqMan™ assays included in the T

The following assays are included in the TrueMark™ Respiratory Panel 2.0, OpenArray™ Plate.

Note: This format 112 TaqMan™ OpenArray™ Plate contains 35 TaqMan™ assays for 32 microbial targets, and the TaqMan™ Universal RNA Spike In/Reverse Transcription (Xeno) Control, plated in triplicate, and assays for the TaqMan™ Universal Extraction Control Organism (B. atrophaeus) and human RNase P RPPH1 gene controls plated in duplicate. Each TaqMan™ OpenArray™ Plate can be used for running up to 23 samples and one control sample.

Table 2 Assays for respiratory tract microbiota

Target organism Assay name Nucleic acid type Assay ID

Bacteria

Bordetella bronchiseptica / par Bordetella pertussis B.pertussis DNA Ba06439623_s1 Chlamydophila pneumoniae C.pneumoniae DNA Ba06439616_s1 Haemophilus inﬂuenzae H.inﬂuenzae DNA Ba06439625_s1 Klebsiella pneumoniae complex K. pneumoniae DNA Ba04932083_s1 Legionella pneumophila L.pneumophila DNA Ba06439617_s1 Mycoplasma pneumoniae M.pneumoniae DNA Ba06439620_s1 Staphylococcus aureus S.aureus DNA Ba04646259_s1 Streptococcus pneumoniae S.pneumoniae DNA Ba06439619_s1

apertussis / pertussis Bordetella DNA Ba06439624_s1

rueMark™ Respiratory Panel 2.0, OpenArray™ Plate

For Research Use Only. Not for use in diagnostic procedures.

Target organism Assay name Nucleic acid type Assay ID

Virus

Adenovirus AdV_1of2 DNA Vi99990001_po Adenovirus AdV_2of2 DNA Vi99990002_po Human Coronavirus 229E CoV_229E RNA Vi06439671_s1 Human Coronavirus HKU1 CoV_HKU1 RNA Vi06439674_s1 Human Coronavirus NL63 CoV_NL63 RNA Vi06439673_s1 Human Coronavirus OC43 CoV_OC43 RNA Vi06439646_s1 Human Enterovirus (pan assay) EV_pan RNA Vi06439631_s1 Human Enterovirus D68 EV_D68 RNA Vi06439669_s1 Human Metapneumovirus (hMPV) hMPV RNA Vi99990004_po Human Parainﬂuenza virus 1 hPIV1 RNA Vi06439642_s1 Human Parainﬂuenza virus 2 hPIV2 RNA Vi06439672_s1 Human Parainﬂuenza virus 3 hPIV3 RNA Vi06439670_s1 Human Parainﬂuenza virus 4 hPIV4 RNA Vi99990005_po Human Respiratory Syncytial Virus A (RSVA) RSVA RNA Vi99990014_po Human Respiratory Syncytial Virus B (RSVB) RSVB RNA Vi99990015_po Human Rhinovirus 1/2 RV_1of2 RNA Vi99990016_po Human Rhinovirus 2/2 RV_2of2 RNA Vi99990017_po Human herpesvirus 4 (HHV4 – Epstein-Barr Virus) HHV4 DNA Vi06439675_s1 Human herpesvirus 5 (HHV5 – Cytomegalovirus) HHV5 DNA Vi06439643_s1 Human herpesvirus 6 (HHV6) HHV6 DNA Vi06439627_s1

Inﬂuenza A Flu_A_pan RNA Vi99990011_po Inﬂuenza A/H1-2009 Flu_A_H1 RNA Vi99990009_po Inﬂuenza A/H3 Flu_A_H3 RNA Vi99990010_po Inﬂuenza B Flu_B_pan RNA Vi99990012_po

Severe Acute Respiratory Syndrome coronavirus 2 (SARSCoV-2); S protein gene

Severe Acute Respiratory Syndrome coronavirus 2 (SARSCoV-2); N protein gene

SARS-CoV2S RNA Vi07918636_s1

SARS-CoV2N RNA Vi07918637_s1

Table 3 Assays for respiratory tract microbiota controls

Control name Assay name Nucleic acid type Assay ID

TaqMan™ Universal Extraction Control Organism (B. atrophaeus)

TaqMan™ Universal RNA Spike In/Reverse Transcription (Xeno) Control

Human RNase P RPPH1 gene RPPH1 DNA Hs04930436_g1

B.atrophaeus DNA Ba06596576_s1

Xeno RNA Ac00010014_a1