Thermo Fisher QS0012, QS0013, QS0014, QS0016 INFORMATION SHEET

QuantiGene Singleplex Assay Kit

Catalog Number QS0012, QS0013, QS0014, and QS0016

Pub. No. MAN0019612 Rev. B.0

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PRODUCT INFORMATION SHEET

This QuantiGene Singleplex Assay Kit contains all of the general
reagents required to run 192, 960, or 4800 QuantiGene Singleplex
assays, depending on the kit. Sample processing kits and target­specific probe sets are sold separately.

About QuantiGene Singleplex Assay

The QuantiGene Singleplex Assay uses branched DNA technology to
quantify RNA directly from a variety of sample types without RNA
purification or target amplification. The QuantiGene Singleplex Assay
is comprised of 3 modules:

• QuantiGene Singleplex Assay Kit

• QuantiGene Singleplex target-specific probe sets

Product use

QuantiGene Singleplex Assay Kit is designed for use with QuantiGene
Singleplex Probe Sets and QuantiGene Sample Processing Kits for
quantitation of target-specific RNA or DNA from cultured cell or whole
blood lysates, fresh, frozen, or FFPE tissue homogenates, or purified
RNA. For more information, refer to the QuantiGene Singleplex Assay
User Manual. For research use only. Not for use in diagnosis of
disease in human or animals.

• QuantiGene Sample Processing Kit (not required for purified RNA
samples)

Contents and storage

Refer to product label for expiration date.

Component Description

Blocking Reagent Aqueous buered solution containing a preservative 400 μL 1.8 mL 10 mL 2–8°C
Pre-Amplifier Solution DNA in an aqueous buered solution 23 mL 120 mL 550 mL 2–8°C
Amplifier Solution DNA in an aqueous buered solution 23 mL 120 mL 550 mL 2–8°C

Capture Plate 96-well polystyrene plate coated with capture probes 2 each
Label Probe Oligonucleotide-alkaline phosphatase conjugate in aqueous

Substrate Chemiluminescent substrate
Label Probe Diluent Aqueous buered solution with a proteincontaining preservative 23 mL 120 mL 550 mL 15–30°C
Lysis Mixture Aqueous buered solution containing a preservative 18 mL 93 mL 410 mL 15–30°C
Wash Buer

Component 1
Wash Buer

Component 2
Plate Seals Day 1 Adhesive backed foil plate seal 2 ea 10 ea 50 ea 15–30°C
Plate Seals Day 2 Adhesive backed foil plate seal 10 ea 50 ea 210 ea 15–30°C

[1]

Captures plates in QS0012 and QS0013 are packaged individually.

[2]

Capture plates in QS0014 are packaged individually. QS0016 has 1 pack of 50 plates.

[3]

Lumigen™ APS-5

Limited product warranty

Life Technologies Corporation and/or its aliate(s) warrant their
products as set forth in the Life Technologies' General Terms and

buered solution

[3]

Aqueous solution

Aqueous buered solution

Conditions of Sale at www.thermofisher.com/us/en/home/global/

terms-and-conditions.html. If you have any questions, please

contact Life Technologies at www.thermofisher.com/support.

Quantity

QS0012

[1]

24 μL 120 μL 550 μL 2–8°C

24 mL 120 mL 530 mL 2–8°C

3 mL 15 mL 75 mL 15–30°C

5 mL 25 mL 125 mL 15–30°C

Quantity

QS0013

10 each

[1]

Quantity

QS0014
QS0016

50 each

Storage

[2]

2–8°C

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The information in this guide is subject to change without notice.
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PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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applicable Limited Use Label Licenses.
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For Research Use Only. Not for use in diagnostic procedures.

16 February 2021