Thermo Fisher BOVIGAM Instruction Manual
|
Catalog Number 7600063
Pub. No. MAN0019748 Rev. A.0
NOT FOR SKIN T ESTING
WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and
WARNING! POTENTIAL BIOHAZARD.
Positive
=
OD Bovine PPD
−
Nil Antigen
≥
0.1; and
OD Bovine PPD
−
Avian PPD
≥
0.1
Negative
OD Bovine PPD
Nil Antigen
0.1; or
OD Bovine PPD
−
Avian PPD
<
0.1
Rev.
Date
Descript ion
• New document. Converted the legacy document
BOVIGAM™ Bovine Tuberculin PPD Stimulating Ag.
INSTRU C TION S FOR U S E
BOVIGAM™ Bovine Tuberculin PPD Stimulating Ag
For the laboratory diagnosis of tuberculosis in animals by the bovine gamma interferon test
gloves. Safety Data Sheets (SDSs) are available from thermofisher.com/support.
appropriate protective eyewear, clothing, and gloves.
Introduction
Applied Biosystems™ BOVIGAM™ Bovine Tuberculin PPD Stimulating Ag is a sterile, preservative-free liquid, containing purified protein derivatives
prepared from the filtrate of a heat-kille d Mycobacterium bovis (Strain AN5) grown on synthetic medium. It has been standardized by Guinea pig potency
testing as described in European Pharmacopoeia and is issued at a strength of 30,000 IU/mL.
General Instructions
It is essential that all blood samples collected for BOV IGAM™ TB Kit and BOVIGAM™ 2G TB K it (bovine gamma interferon tests) testing are taken from a
cleaned site into a tube containing heparin as anticoagulant. Gently invert the tubes several times to ensure complete mixing of blood and heparin. Blood
samples should be transported to the testing laboratory at ambient temperature (22±3°C; avoid extreme s) and used in the BOVIGAM
BOVIGAM
IMPORTANT! Blood must not be frozen or stored in a refrigerator.
™
2G TB Kit assays with in 30 hours of collection.
Read the biological hazard safety information at this product’s page at thermofisher.com. Wear
™
TB Kit and
BOVIGAM™ (bovine gamma interferon) tests
BOVIGAM™ TB Kit and BOVIGAM™ 2G TB Kit are rapid, blood-base d
assays of cell mediated immunity (CMI) for the diagnosis of bo vine
tuberculosis in cattle and other ruminants. Tuberculin PPD is mixed with
lymphocytes in whole blood and incubated overnight at 37°C. Blood
plasma is then collected from above the cultures and assayed for
interferon-γ (IFN-γ) production using the BOVIGAM
BOVIGAM
™
2G TB Kit enzyme immunoassay (EIA).
™
TB Kit or
General precautions
Laboratory safety
National Safety Regulations must be strictly followed.
Usage
For stimulation in 96-well cell-cult ure plates:
Dilute 110 µL of BOVIGAM
890 µL of RPMI medium for a final assay concentration of 300 IU/m L. Mix
blood samples evenly immediately before use. For comparative testing,
dispense three 250 µL aliquots of blood into culture vessels. To one aliquot,
add 25 µL of diluted stock and mix thoroughly. To the other aliquots, add
25 µL of sterile PBS to one and 25 µL of BOVIGAM
Stimulating Ag (Cat. No. 7600067) to the other. Aseptic procedures should
be follo wed using a sterile pipetting apparatus.
For stimulation in 24-well cell culture plates:
Dilute 160 µL of BOVIGAM
840 µL of RPMI medium for a final assay concentration of 300 IU/m L. Mix
blood samples evenly immediately before use. For comparative testing,
dispense three 1.5 mL aliquots of blood into culture vessels. To one aliquot,
add 100 µL of diluted stock and mix thoroughly. To the other aliquots add
100 µL of nil control (sterile PBS) to one and 100 µL of BOVIG AM
Tuberculin PPD Stimulating Ag (Cat. No. 7600067) to the other. Aseptic
procedures should be followed using a sterile pipetting apparatus.
™
Bovine Tuberculin PPD Stimulating Ag in
™
Avian Tuberculin PPD
™
Bovine Tuberculin PPD Stimulating Ag in
™
Avian
Interpretation
(See also the respective Instructions for Use of the BOVIGAM™ TB Kit and
BOVIGAM
Compare the absorbance values of the nil antigen, avian, and bovine PPD
samples for each animal.
™
2G TB Kit for further details.)
=
−
<
Retesting
Retesting may be performed at any time after the last test.
Storage
Store, protected from light, at 2–8°C. Do not freeze.
Presentation
BOVIGAM™ Bovine Tuberculin PPD Stimulating Ag is avai lable in 5-mL vials.
Customer and technical support
Technical support: visit thermofisher.com/askaquestion
Visit thermofisher.com/support for the latest in services and support,
including:
• Worldwide contact telephone numbers
• Order and web support
• User guides, manuals, and protocols
• Certificates of Analysis
• Safety Data Sheets (SDSs; also known as MSDSs)
NOTE: For SDSs for reagents and chemicals from other manufacturers,
contact the manufacturer.
Limited product warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products
as set forth in the Life Technologies' General Terms and Conditions of Sale
found on Life Technologies' website at www.thermofisher.com
/us/en/home/global/terms-and-conditions.html. If you have any questions,
please contact Life Technologies at thermofisher.com/support.
Prion ics Lelystad B.V. | Platinastraat 33 | 8211 AR Lelystad | The Netherlands
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILI ATE(S)
WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR
CONSEQUENTIAL DAMAGES IN C ONNECTION WITH OR ARISING F ROM THI S DOCUMENT,
INCLUDI NG YOUR USE OF I T.
Revision history of Pub. No. MAN0019748 (Eng lish )
(7600063_Bovigam_Lelystad_Tuberculin_PPD_Bovine_v1.0_e.pdf) to
10 November
A.0
Important Licensing Information: These products may be covered by one or more Limited Use Label
Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use
Label Licenses.
©2020 Thermo Fisher Scient ific Inc. All rights reserved. All trademarks are the property of Thermo
Fisher Scientific and its subsidiaries un less otherwise specified.
2020
the current document template, with associated updates to the
publication number, limit ed license information, warranty,
trademarks, and logos.
• The product name was changed from Bovine Tuberculin PPD 3000 to
For Veterinary Use Only. For In Vitro Use Only.
thermofisher.com/support
thermofisher.com
10 November 2020
thermofisher.com/askaquestion
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