Teledyne ISCO TN11 Instruction Manual

Adjustable Rf Solid
Load Cartridge Cap for
Redi

Sep

Product Information and Instructions

®

Rf Cartridges

Chromatography Technical Note

TN11, June 2020

Overview

Teledyne ISCO’s adjustable Rf solid load cartridge
caps are easy to use and provide you with additional
flexibility in loading your solid samples.

Three cap sizes are available (refer to Table 1) to fit
5, 25, and 65 gram RediSep Rf empty or prepacked solid
load cartridges for use with the CombiFlash® system.

Designed for Convenience

The design of the solid load sample cartridge cap is
simple, yet effective.

● Easily adjustable – A lever on the cap provides

for quick vertical movement of the metal plunger
rod, allowing for adjustment to any bed height.

● No dead volume – Eliminating dead volume

improves gradient accuracy because there is no
space for solvent mixing.

● Secure lock – The sleeve’s internal locking

mechanism holds the plunger and cartridge
securely in place, without the use of clumsy
external springs and locking mechanisms.

● Low maintenance – The plunger uses a virgin

PTFE seal, eliminating costly, leak-prone O-rings.

● No leaks – Internal stops within the cap keep the

cartridge from rotating. The sleeve tightly holds
the cartridge and prevents leaks, even under
high operating pressures.

● High operating pressure – The cap, when used

according to these instructions, is rated to
200 psi.

Preparing an Empty Cartridge

1. Adsorb the sample onto loose silica or other
suitable media.

2. Pour the sample and media mixture into the car­tridge (Figure 1).

3. Place a frit into the cartridge.

4. Push the frit down on top of the sample and
media mixture.

5. If you did not remove the solvent before loading
the mixture into the cartridge, continue with Dry-
ing the Cartridge.

Preparing Cartridges

Teledyne Isco’s Rf adjustable cartridge cap can be
used with any type of sample, and are effective with low
solubility samples. The adjustable caps can be used
with a variety of RediSep Rf cartridges. To meet your
specific needs, RediSep Rf disposable cartridges are
available empty, prepacked with silica gel, or prepacked
with C18 reversed phase media. After selecting a car­tridge, prepare the solid sample.

Preparing a Prepacked Cartridge

1. Dissolve the sample and introduce it onto the
prepacked cartridge (Figure 1).

2. Remove the solvent by drying the cartridge.

Figure 1: Preparing Cartridges

prepacked cartridges (left) or pour adsorbed sample into
empty cartridges (right).

Pipette

sample onto

Drying the Cartridge

Solvent should be removed from the prepared car­tridge. You can do so using a vacuum dryer or the air
pump in the CombiFlash system. Instructions to use
the system’s air pump are included in the next sections,
Loading Cartridges.

Chromatography Technical Note #11 69-5233-423

Loading 5 and 25 gram Cartridges

To load the 5 or 25 gram cartridge on the system:

1. The solid load cartridge cap must match the size
of your selected cartridge. If it does not match,
install the correct cap now.

2. Press the lever on the side of the cap and fully
extend the plunger (Figure 2).

3. Slide the plunger into the cartridge until it
reaches the top frit (Figure 3).

4. Press the lever and push the cartridge into the
cap. Align the cartridge so that it fits fully into
the recess in the cap (Figure 4).

2

1

CAUTION

Operating the cartridges at pressures greater than 50 psi
may cause leaks.

1

Figure 2: Press the lever and extend the plunger

2

Figure 4: Align and push cartridge into the cap

Figure 3: Insert the plunger to the bed