Teledyneisco PurIon User Manual

PurIon Mass Spec for
Flash and Prep Labs

Model

10 to 1,200 Da (m/z)

S

L

For small molecule purification

Model

10 to 2,000 Da (m/z)

For large molecule purification

ACCQPrep and CombiFlash Integration

Teledyne ISCO flash and prep chromatography systems are PurIon MS ready. With
PeakTrak integration no additional software or processing is required.

•Active flow splitting for accurate, reproducible results with low sample loss, high
sensitivity, while minimizing sample buildup in MS

•Combine MS with UV, UV/Vis and ELSD for
compete sample characterization

•Combine detector signals for high purity collection

•Save time and solvent with built-in Flash Terminate
on Target algorithm and Prep Focused Gradient
Generation

•Provide full mass spectra for contents of any
fraction tube to determine fraction purity

•Automatically cleans the mass spectrometer
interface

PurIon shown with the ACCQPrep HP150 HPLC system.

The PurIon Mass Spectrometer is designed to provide highly affordable mass-directed fractionation to labs performing flash and prep
purifications on Teledyne ISCO systems. The PurIon interfaces seamlessly with the CombiFlash® NextGen, CombiFlash® EZ Prep, and
ACCQPrep® to instantly verify the identity of target compounds. Our innovative PeakTrak® software provides single point of control, three
predetermined ionization settings, and the ability to customize ionization parameters to maximize signal strength. Use the convenient Ion
Finder utility to identify fragments and adducts of your compound and optimize detection parameters from directly injected samples prior
to purification. Highly accessible and easy-to-use, PurIon is ideal for chemists wanting high-quality mass spectral data without the need for
specialized training.

Single Quadrupole Mass Analyzer

The Teledyne ISCO PurIon is a compact, single-quadrupole
mass analyzer with an atmospheric pressure interface optimized
for chromatography purification. PurIon provides highly reliable
molecular weight information about target compounds as well
as other adducts during the purification run—or by direct-injection
analysis prior to purification—in positive ion mode via proton
attachment (M+H)+ and negative ion mode via proton removal
(M-H)-

Versatile and Reliable

Co-eluting peaks are easily isolated by mass selectivity based
on mass-to-charge ratio (m/z) of the target analyte. With both
electrospray ionization (ESI) and atmospheric pressure chemical
ionization (APCI) capabilities, PurIon delivers rapid and reliable
confirmation of compounds in normal-phase and reverse-phase
applications. Detect both small and large molecules with extended
mass range from 10 Da to 2000 Da.

PurIon shown with the CombiFlash NextGen flash system.

ESI vs. APCI

PurIon provides both ESI (electrospray
ionization) and APCI (atmospheric pressure
chemical ionization) capabilities.

•ESI for compounds that are typically polar,
ionizable, and either acidic or basic

•APCI for typically polar and some non-polar
compounds; analyte must have sufficient
proton affinity to be protonated.

Method Development and
Fraction Product Verification

•Direct injection capability for method

development, product verification of
fractions, or quick monitoring of
reaction progress.

teledyneisco.com 21 teledyneisco.com