Teledyne Isco HP125, HP150 User Manual

Guide to Different Loading
Methods for the
ACCQPrep HP125 & HP150

Overview

The ACCQPrep HP125 and HP150 are is a HPPLC

(High Performance Preparative Liquid Chromatography)
systems that offers several different variables regarding
loading techniques. These include: choice of sample
loop size, AutoInjector Module or manual injection, and
the use of an AutoSampler Module.

This technical note is to help summarize the advan­tages and disadvantages of each method and t
direction to more detailed Application or Technical
Notes as necessary.

Sample Loop Size

The AccqPrep comes with a 5mL loop although it
can accommodate loops of different sizes.

Loop Size Part Number

5 mL 299125062

Guidelines for Loop Size

100 μL and 1 mL —

loop size in order to avoid sample loss during the
loading process (either Auto or Manual).

Advantages:

• Useful at low flow rates to avoid large sample

loop delay.

• Minimized sample dilution.

• Useful for method development on 4.6 mm

diameter columns.

tions:

Limita

• Can’t load more than 50% the loop size without

sample loss.

• Limited loading capacity.

Above 1 mL —

Module it is possible to load up to 1 mL less than the
sample loop size (ie 4 mL for 5 mL loop; 9 mL for
10 mL loop; and 19 mL for 20 mL loop). If injecting
manually, avoid loading more than 50% the loop size to
avoid sample loss due to variation in operator reproduc­ibility, precision, and accuracy.

Advantages:

• Increased loading capacity.

• Increased amount of sample loop available when

AutoInjector or AutoSampler Module.

using

Limitations

• Significant sample loop delay at low flow rates.

• Possible band broadening as loop increases.

Avoid loading more than 50% of the

If using an AutoInjector or AutoSampler

o give

Chromatography Technical Note

Oct 2020, TN41

For loading size optimization you can refer to Appli­cation Note #XX Scouting Pause II: Optimizing Loading
Size for more detailed suggestions.

You can refer to Technical Note 43

Loop Maximum Injection Volume

sion of the injection volume limitations for different loop
sizes.

for a more detailed discus-

ACCQPrep Sample

AutoInjector Module

The AutoInjector Module offers a reliable method for
automated repeated injections of the same sample. Its
consistency from injection to injection not only allows
multiple injections without user input, but also a repro-

ducible sequence that gives accuracy down to a 10 μL

injection volume. This gives superior results over
manual injection techniques where user variability and
experience provide inconsistent results.

Advantages:

• Multiple Injections without user feedback.

• Improved peak shape and separation.

• Reproducibility.

• Injection volume down to 10 μL.

Limitations:

• Only one sample at a time.

• Only one injection volume per series of runs.

• Automated wash sequence requires user action.

AutoSampler Modu le

The AutoSampler module expands upon the advan­tages and features of the AutoInjector Module. In
addition to all the benefits of the AutoInjector, the
AutoSampler offers the ability to queue different sam­ples, set up different injection volumes of the same
sample, and an automated wash sequence without user
action. Additionally, it increases the fraction collection
capacity of the ACCQPrep syste

Advantages:

• Multiple different samples.

• Allows different injection volumes or chromato-

graphic methods of the same sample.

• Additional scouting pause feature to optimize

conditions.

• Completely automated wash process.

• Additional fraction collection capacity in addition

to the benefits of the AutoInjector Module.

m.

Chromatography Technical Note Oct 2020, TN41

You can refer to Technical Note 44 Minimum Injection

Volume on the ACCQPrep for more detailed information.

Manual Injection

Luer Adapter Port (

injection of sample into the sample loop via syringe.

Limitations:

• Potential loss of sample if port is not flushed after

injection.

• Injection volume shouldn’t exceed 50% of the loop

size using this method no matter the loop due to user
variation and experience.

• Inferior reproducibility and technique compared to

AutoInjector module.

Note

Check valve is required on the waste port of the
injection valve in order to avoid leakage out of the
injection port after injection.

Valco p/n ZLA-1

) —

Allows manual

Teledyne Isco

P.O. Box 82531, Lincoln, Nebraska, 68501 USA

Toll-free: [phone] • Phone: [phone] • Fax: [phone]

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Teledyne Isco is continually improving its products and reserves the right to change product
specifications, replacement parts, schematics, and instructions without notice.