Teledyne BioOptix 10 User Manual
BioOptix™ 10
Installation and Operation Guide
#69-5203-150
Copyright © 2004 Teledyne Isco, Inc.
All rights reserved.
Revision B, September 20, 2005
Foreword
This instruction manual is designed to help you gain a thorough understanding of the
operation of the equipment. Teledyne Isco recommends that you read this manual
completely before placing the equipment in service.
Although Teledyne Isco designs reliability into all equipment, there is always the possibility of a malfunction. This manual may help in diagnosing and repairing the malfunction.
If the problem persists, call or e-mail the Teledyne Isco Technical Service Department
for assistance. Simple difficulties can often be diagnosed over the phone.
If it is necessary to return the equipment to the factory for service, please follow the
shipping instructions provided by the Customer Service Department, including the
use of the Return Authorization Number specified. Be sure to include a note
describing the malfunction. This will aid in the prompt repair and return of the
equipment.
Teledyne Isco welcomes suggestions that would improve the information presented in
this manual or enhance the operation of the equipment itself.
Teledyne Isco is continually improving its products and reserves the right to
change product specifications, replacement parts, schematics, and instructions without notice.
Customer Service
Phone: (800) 228-4373 (USA, Canada, Mexico)
Fax: (402) 465-3022
Email: IscoCSR@teledyne.com
Technical Service
Phone: (800) 775-2965 (Analytical)
Email: IscoService@teledyne.com
Return equipment to: 4700 Superior Street, Lincoln, NE 68504-1398
Other Correspondence
Mail to: P.O. Box 82531, Lincoln, NE 68501-2531
Email: IscoInfo@teledyne.com
Web site: www.isco.com
Contact Information
(402) 464-0231 (Outside North America)
(800) 228-4373 (Samplers and Flow Meters)
Revised September 15, 2005
BioOptix™ 10
Safety
BioOptix™ 10
Safety
General Warnings Before installing, operating, or maintaining this equipment, it is
imperative that all hazards and preventive measures are fully
understood. While specific hazards may vary according to location
and application, take heed in the following general warnings:
WARNING
Liquids associated with this instrument may be classified
as carcinogenic, biohazardous, flammable, or radioactive.
Should these liquids be used, it is highly recommended that
this application be accomplished in an isolated
environment designed for these types of materials in
accordance with federal, state, and local regulatory laws,
and in compliance with your company’s chemical/hygiene
plan in the event of a spill.
AVERTISSEMENT
Eviter de répandre des liquides dangereux. Les liquides qui
sont analysés dans cet instrument peuvent être
cancérigènes, hasards biologiques, inflammables, ou
radioactifs. Si vous devez utiliser tels liquides, il est très
recommandé que vous le faites à l'intérieur d'un
environnement isolé conçu pour tels liquides. Cet
environnement isolé devrait être construit selon les
règlements fédéraux, provinciaux, et locaux, aussi que le
plan de votre compagnie qui concerne l'évènement d'un
accident avec les matières hasardeuses.
WARNING
Avoid hazardous practices! If you use this instrument in
any way not specified in this manual, the protection
provided by the instrument may be impaired.
AVERTISSEMENT
Éviter les usages périlleux! Si vous utilisez cet instrument
d’une manière autre que celles qui sont specifiées dans ce
manuel, la protection fournie de l’instrument peut être
affaiblie; cela augmentera votre risque de blessure.
WARNING
If this system uses flammable organic solvents, Teledyne
Isco recommends that you place this system in a
well-ventilated environment, designed for these types of
materials. This environment should be constructed in
accordance with federal, state, and local regulations. It
should also comply with your organization’s plan
concerning chemical and hygiene mishaps. In all cases use
good laboratory practices and standard safety procedures.
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BioOptix™ 10
Safety
AVERTISSEMENT
Ce système peut utiliser des dissolvants organiques
inflammables. Pour réduire le péril qui peut être causé par
l'accumulation des vapeurs explosives, Teledyne Isco
recommande que vous installez ce système dans un
environnement bien-aéré qui est conçu pour les matières
hasardeuses. Cet environnement devrait être construit
selon les règlements fédéraux, provinciaux, et locaux.
Aussi, il devrait se conformer au plan de votre organisation
qui concerne les mésaventures de l'hygiène ou de
chimique. En tout cas, utilisez toujours de pratiques
bonnes de la laboratoire et des procédures standardes de
la sûreté.
Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts.
Three levels of severity are described in the sample alerts below.
CAUTION
Cautions identify a potential hazard, which if not avoided, may
result in minor or moderate injury. This category can also warn
you of unsafe practices, or conditions that may cause property
damage.
WARNING
Warnings identify a potentially hazardous condition, which
if not avoided, could result in death or serious injury.
DANGER
DANGER – limited to the most extreme situations
to identify an imminent hazard, which if not
avoided, will result in death or serious injury.
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BioOptix™ 10
Hazard Symbols The equipment and this manual use symbols used to warn of
hazards. The symbols are explained below.
Hazard Symbols
Warnings and Cautions
The exclamation point within the triangle is a warning sign alerting you of
important instructions in the instrument’s technical reference manual.
The lightning flash and arrowhead within the triangle is a warning sign alerting you of “dangerous voltage” inside the product.
Symboles de sécurité
Ce symbole signale l’existence d’instructions importantes relatives au produit dans ce manuel.
Ce symbole signale la présence d’un danger d’électocution.
Safety
Warnungen und Vorsichtshinweise
Advertencias y Precauciones
Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie darauf
aufmerksam macht, daß wichtige Anleitungen zu diesem Handbuch
gehören.
Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das Sei vor “gefährlichen
Spannungen” im Inneren des Produkts warnt.
Esta señal le advierte sobre la importancia de las instrucciones del manual
que acompañan a este producto.
Esta señal alerta sobre la presencia de alto voltaje en el interior del
producto.
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BioOptix™ 10
Safety
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BioOptix™ 10
Table of Contents
Section 1 Introduction
1.1 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.3 System Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.3.1 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.3.2 BioOptix 10 Left-side Controls and Connections . . . . . . . . . . . . . . . . . . . . . 1-4
1.3.3 BioOptix 10 Right-side Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1.3.4 BioOptix 10 Rear Panel Controls and Connections . . . . . . . . . . . . . . . . . . . 1-6
Section 2 Installation
2.1 Unpacking the BioOptix 10 System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 BioOptix 10 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2.1 BioOptix 10 Location and Space Requirements . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2.2 Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.2.3 Assembling the BioOptix 10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.2.4 Assembling the Foxy 200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
2.2.5 Fluid Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
2.2.6 Windows PC Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
2.3 BioOptix 10 Software Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
2.3.1 Configuring BioOptix 10 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
2.4 BioOptix 10 System Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15
Section 3 Operation
3.1 Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Column Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2.1 Column Tubing and Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.3 BioOptix 10 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.3.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.4 Gradients . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.4.1 Defining a Gradient . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.4.2 Saving the Gradient . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.5 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.5.1 Equilibrate Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.5.2 Load Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3.5.3 Wash Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.5.4 Elute Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.5.5 Flush Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.5.6 Saving the Method File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.6 Experiments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.6.1 Setting up the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3.6.2 Editing the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3.6.3 Saving the Experiment File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3.7 Running the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3.7.1 System Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3.7.2 Operation Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.7.3 Suspending System Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
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Table of Contents
3.8 Post-experiment Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.8.1 Pump Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.8.2 Saving Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3.8.3 AutoReports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
Section 4 System Maintenance
4.1 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.2 Exterior Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.3 Piston Seal Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.4 Deuterium Lamp Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.5 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.5.1 Operating Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.5.2 Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.5.3 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.5.4 Inaccessible Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.5.5 Inaccessible Breakers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.6 Service Department . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
4.7 How to Ship Returns. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Appendix A Method Templates
A.1 Opening the Template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
A.2 Modifying the Template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
A.2.1 General Editing Rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
A.2.2 [Blocks] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-4
A.2.3 [Blockn] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-4
A.2.4 [Other] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-4
Appendix B Fluid Path Diagrams
B.1 Fluid-type Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
B.2 Load-type Blocks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B.3 Gradient-type Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
B.4 Pump Flush. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
B.5 Plumbing Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
Appendix C Replacement Parts Lists
C.1 Detector Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3
C.2 Column Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-7
C.3 Pump Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-11
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Table of Contents
List of Figures
1-1 BioOptix 10 front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1-2 BioOptix 10 left-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
1-3 BioOptix 10 right-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1-4 BioOptix 10 rear panel controls and connections . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
2-1 System space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2-2 Accessing pump and column module internal connections . . . . . . . . . . . . . . . . . . 2-3
2-3 Removing the column module back plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2-4 Routing the Sample Out lines (column module) . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2-5 Pump controller connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
2-6 P110 Connection on BD3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2-7 J106 Connection on BD1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2-8 AC power connection from pump module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2-9 Ribbon cable connections
(top half of column module shown) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2-10 Liquid connections
(pump module shown) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
2-11 Aligning the fasteners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2-12 Column clamps inserted . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2-13 Foxy arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
2-14 Buffer assembly routes a common solution to all ten channels . . . . . . . . . . . . . 2-11
2-15 Buffer assemblies installed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
2-16 Foxy 200 tubing connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
2-17 Adhesive retainers attached . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13
2-18 Column bypass tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
2-19 Mode control knob . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16
2-20 BioOptix 10 Manual Hardware Control window . . . . . . . . . . . . . . . . . . . . . . . . 2-17
3-1 Gradient File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3-2 Equilibrate tab settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3-3 Load tab settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3-4 Wash tab settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3-5 Elute tab settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3-6 Flush tab settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3-7 Experiment File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3-8 BioOptix 10 Operation Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3-9 Pause at end of block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3-10 Pump flush option . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3-11 Flush window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
4-1 Flush window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4-2 Access the pump assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4-3 Pump assembly components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4-4 Attach seal to push rod . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4-5 Waste port mounting plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
4-6 Deuterium lamp location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
4-7 Circuit breaker location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4-8 Thermal breaker location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
A-1 Installed Standard.tpl file . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
A-2 Customizable values in the Standard.tpl file . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
B-1 Fluid path for Fluid-type blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
B-2 Inactive channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B-3 Fluid path for Load-type blocks -
Pump injections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
B-4 Fluid path for Load-type blocks -
Manual injections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
B-5 Fluid path for Gradient-type blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-3
B-6 Pump flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
B-7 BioOptix 10 Plumbing Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-5
ix
BioOptix™ 10
Table of Contents
List of Tables
1-1 BioOptix 10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1-2 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1-3 BioOptix 10 Left-side Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
1-4 BioOptix 10 Right-side Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1-5 BioOptix 10 Rear Panel Controls and Connections . . . . . . . . . . . . . . . . . . . . . . . . 1-6
2-1 Tubing Color chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12
2-2 Windows
3-1 Operation Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
4-1 Common Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
®
PC Minimum Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
x
BioOptix™ 10
Section 1 Introduction
1.1 Product Overview The BioOptix™ 10 system performs automated protein purifi-
cation on as many as ten liquid samples simultaneously. The
system has ten independent pumps that keep flow rates properly
regulated in each channel, and ten independent detectors so that
you can monitor the separation in every channel. The purified
sample is collected by a fraction collector — up to 300 fractions
per sample for unattended operation, or unlimited fractions for
attended purification runs. BioOptix 10 control software lets you
quickly locate the fractions of interest.
System highlights include:
• Individual detector on each channel.
• Unique gradient conditions can be used for each
channel— great for scouting ideal gradient conditions.
• Repeatable, uniform flow for each sample, regardless of
buffer gradient or sample consistency. Ten independent
precision pumps provide flow rates from 0.5 to 12
mL/min for each channel.
• Fast and easy sample loading—individual sample introduction lines for each channel.
• Compact size gives you maximum capability in
minimum bench space.
• High fraction capacity —
❍ collect up to 300 fractions per sample completely
unattended,
❍ or unlimited fractions by manually replacing filled
racks during a run.
• Pumps designed for salt buffers and common solvents
ensure long life, low maintenance, no seal rinse fluid
needed.
1-1
BioOptix™ 10
Section 1 Introduction
1.2 System Specifications Table 1-1 lists the BioOptix 10 specifications.
Table 1-1 BioOptix 10 Specifications
Dimensions
Weight
Power Requirement
Line Frequency 50 or 60 Hz
Operating Temperature
Humidity
Gradient Flow Rate Range 0.5 to 12 mL/min
Flow Rate Accuracy ± 5% full scale (using water)
Gradient Accuracy ± 5% full scale (using water)
Gradient Linearity ± 5% full scale (using water)
Operating Pressure 0 to 6.895 bar (0 to 100 psi)
a
a,b
a,c
The power switch is the
disconnect device.
a
a
Height: 69 cm
Width: 72 cm
Depth: 61 cm
210 kg
100 VAC ±10%, 2.0 Amps
117 VAC ±10%, 2.0 Amps
234 VAC ±10%, 1.0 Amps
5 to 40°C
When connected to mains power, 95% relative humidity maximum at 5 to
40°C
Flow Cell Path Length 2 mm
Detector Wavelength 190 to 360 nm
-4
Detector Noise 5 × 10
Detector Drift 1 × 10
CE Conformity Specifications
a,d
Pollution Degree: 2
AU
-3
AU/hr
Installation Category: II
Maximum Altitude: 2000 meters
a. Excludes Foxy 200 Fraction Collector and Windows PC Controller. Refer to the respective manuals for these additional
specifications.
b. Dry weight of the BioOptix 10, excluding connection cables, tubing, and user-supplied buffer containers.
c. Operating voltage of the BioOptix 10 is factory set.
d. Refer to the CE Declaration of Conformity, at the back of this manual, for applicable standards and test results.
1-2
1.3 System Controls and Connectors
1.3.1 BioOptix 10 Front Panel Controls
BioOptix™ 10
Section 1 Introduction
1
2
3
4
5
Figure 1-1 BioOptix 10 front panel
Table 1-2 BioOptix 10 Front Panel Controls
Item Name Description
1 Manual Sample Inject Port Injection port for syringe-injected liquid samples.
2 Mode Knob Configures fluid path for purging, manual sample injection, or purification
operation.
3 Column Inlet Port Allows connection of tubing to column inlet.
4 Column Clamp Position Adjust Secures column clamp and allows for horizontal and rotational positioning of
columns.
5 Column Outlet Port Allows connection of tubing from column outlet.
6 Column Clamp (Not shown) Tightens to secure the chromatography column.
1-3
BioOptix™ 10
Section 1 Introduction
1.3.2 BioOptix 10 Left-side Controls and Connections
1
2
4
3
5
6
7
Figure 1-2 BioOptix 10 left-side connections
Table 1-3 BioOptix 10 Left-side Connections
Item Name Description
1 Buffer A Inlet Ports Ports to accept Buffer A solution for each channel.
2 Buffer B Inlet Ports Ports to accept Buffer B solution for each channel.
3 Sample In Ports Ports to accept sample for each channel.
4 Pump Purge Port When the Mode Knob is in the Purge Pump position, pumped fluids are
diverted to this port, bypassing the column, detection, and collection.
5 Waste Ports Connects to waste collection container(s). Connecting to separate contain-
ers allows isolation of waste buffers by each channel, which could be beneficial should a purified sample fail to collect.
6 Internal Drain Fluid from internal leaks exit the case through this opening. Internal leaks
are the result of pump seal failures due to neglected routine maintenance.
7 Power Switch I = On
0= Off
1-4
1.3.3 BioOptix 10 Right-side Connections
BioOptix™ 10
Section 1 Introduction
1
Figure 1-3 BioOptix 10 right-side connections
Table 1-4 BioOptix 10 Right-side Connections
Item Name Description
1 Purified Sample Outlet Channels 1 through 10 which connect to the Foxy 200 Fraction Collector.
1-5
BioOptix™ 10
Section 1 Introduction
1.3.4 BioOptix 10 Rear Panel Controls and Connections
1
2
Figure 1-4 BioOptix 10 rear panel controls and
connections
Table 1-5 BioOptix 10 Rear Panel Controls and Connections
Item Name Description
1 Cooling Fan Ventilates the BioOptix 10 case and cools internal components.
2 External Control Connector Connects to the Foxy 200 Fraction Collector and computer.
3 Fuses Rear Panel Accessible Fuses.
Must always be replaced with same type fuses:
100/117 VAC — 3 amp “T” type fuse
234 VAC — 1.5 amp “T” type fuses
Internal fuses are not accessible to the user and should only be
replaced by a trained service technician.
Pump Drive Board F101 — 4.0 amp “T” type fuse.
Power One PS F1 — Not Serviceable.
Lamp Drive Board F1 — .375 amp “T” type fuse.
Case Bottom Assy F4 — Not Serviceable (T2 primary).
F5 — Not Serviceable (T2 secondary).
F6 — Not Serviceable (T2 secondary).
3
4
The Column Module has an internal 1.2 amp circuit breaker and a thermal breaker that should only be reset by a trained service technician.
4 AC Power Connector Connects to AC power source. A dedicated power source is recommended.
Mains power cord is the disconnect device.
1-6
BioOptix™ 10
Section 2 Installation
This section provides instructions for installing the system and
preparing it for operation.
The BioOptix 10 system consists of a BioOptix 10 unit, a
Foxy 200 Fraction Collector, and a Windows PC running
BioOptix 10 software.
The system is heavy. Use a two-person lift to prevent injury.
WARNING
2.1 Unpacking the BioOptix 10 System
2.2 BioOptix 10 Installation
2.2.1 BioOptix 10 Location and Space Requirements
Carefully unpack the shipment and inspect the contents to verify
receipt of all components against the packing list. If there is any
damage to the shipping carton or any components, contact the
shipping agent and Teledyne Isco (or its authorized representative) immediately.
WARNING
If there is any evidence that the system has been damaged
in shipping, do not plug it into the power line. Contact
Teledyne Isco or its authorized representative for advice.
Compare the contents of the boxes with the enclosed packing slip.
If there are any shortages, contact Teledyne Isco immediately.
The system should be installed on a work surface capable of supporting the system, and buffer, sample, and waste containers.
The system is designed for ambient temperature and cold-room
operation. Refer to Table 1-1 for environmental specifications.
The BioOptix 10 unit requires about 3350 square centimeters
(520 in
2-1. Space must be left behind the unit for case ventilation and
electrical connections. The left side of the unit should be kept
clear to access the power switch and for fluid connections.
2
) of bench space. Space requirements are shown in Figure
CAUTION
Ensure there is at least 10 cm (4 inches) of air space on its left
side so that the disconnect device (power switch) may be easily accessed in an emergency.
2-1
BioOptix™ 10
Section 2 Installation
= Keep area clear for instrument
connections, ventilation and to
access On/Off Switch.
To keep tubing length at a minimum, buffer, sample, and waste
collection containers should be placed to the left of the unit. The
containers may also be kept behind the unit as long as the containers are accessible.
The Foxy 200 should be installed to the right of the BioOptix 10.
The fraction collector requires about 1800 square centimeters
(275 in
2
) of bench space. Ensure that the full motion of the
fraction collector arm is not obstructed.
The Windows PC may be placed anywhere within 2 meters of the
BioOptix 10 unit (the length of the serial control cable).
100 cm
16 cm
8 cm
33 cm
56 cm
13 cm
10 cm
10 cm
Figure 2-1 System space requirements
2.2.2 Electrical Requirements
The operating voltage of the BioOptix 10 is factory set. Refer to
the serial number label on the right side panel to determine the
electrical requirements.
Teledyne Isco recommends that the BioOptix 10 system runs on
a dedicated AC circuit to prevent abnormal conditions that may
be caused by high current or electrical noise. The BioOptix 10
and Foxy 200 should be connected to the same circuit to prevent
ground loop anomalies.
(Rear)
BioOptix
(Front)
44 cm 29 cm
12 cm
Foxy 200
50 cm
16 cm
8 cm
61 cm
2.2.3 Assembling the BioOptix 10
2-2
The BioOptix 10 is shipped in two pieces— a column module and
a pump module. After unpacking and inspecting the modules
place them as shown in Figure 2-2 on a sturdy work surface. This
will allow access to the internal electrical and fluid connections.
Section 2 Installation
Pump Module Column Module
BioOptix™ 10
Figure 2-2 Accessing pump and column module internal
connections
To complete the connections:
1. Release the two sets of tubes from the retaining clips (Figure 2-3).
2. Remove the four screws securing the back metal plate on
the column module (Figure 2-3). Then, lift and remove the
plate.
Screws Screws
Retaining
Clips
Figure 2-3 Removing the column module back plate
2-3
BioOptix™ 10
Section 2 Installation
3. While facing the back of the column module, locate the two
liquid tubing bundles secured to the lower-left inside case
panel. Route these two bundles through the slots at the
edge of the panel (Figure 2-4).
Route tubing
through slots
To
Foxy 200
Figure 2-4 Routing the Sample Out lines (column
module)
4. Connect the beige 25-pin connector from the pump module
to the column module connector labeled Pump Controller.
Refer to Figure 2-5.
From Pump
Module
Figure 2-5 Pump controller connection
2-4
BioOptix™ 10
Section 2 Installation
5. Connect the orange connector labeled BD3 P110 to P110 on
the Power Drive Circuit Board (Figure 2-6).
From Pump
Module
Figure 2-6 P110 Connection on BD3
Figure 2-7 J106 Connection on BD1
6. Connect the plug labeled BD1 J106 to J106 on the Controller Circuit Board (Figure 2-7).
From
Pump
Module
2-5
BioOptix™ 10
Section 2 Installation
7. Plug the black power cord from the pump module into the
receptacle at the lower part of the column module (Figure
2-8).
From
Pump
Module
Figure 2-8 AC power connection from pump module
8. Before completing the remaining cable connections, replace
the back metal plate on the column module. The openings
in the plate will fit around the connections made in steps 3
through 7. Secure the plate with the four screws (Figure
2-3). Then, reattach the tubing to the retaining clips.
9. The remaining ribbon cable connections are routed from
the top of the pump module through the opening on the
back plate of the column module. Refer to Figure 2-9 and
complete the connections, matching the connector labels.
P6
Not
Used
P12
P8 P11
Figure 2-9 Ribbon cable connections
(top half of column module shown)
2-6
Tubing to pump
module
BioOptix™ 10
Section 2 Installation
10. Connect the tubing from the column module to the pump
module. Refer to Figure 2-10.
a. Remove the two thumbscrews securing the splash
guard to the pump module. Set aside the splash guard.
Note
The tubing numbers from the column module match the numbers above the compression fittings on the pump module. (The
tubing is visible in Figure 2-9; the compression fittings are visible in Figure 2-10.)
b. Loosen the nut on the compression fitting, insert the
tubing fully into the fitting and finger-tighten the nut.
Then, use a wrench to tighten the nut an additional full
turn. Repeat for all ten channels.
c. Replace the splash guard so that the tubing is routed
up and over the guard.
Figure 2-10 Liquid connections
(pump module shown)
Fittings for column
module tubing
Splash Guard
Thumbscrew
11. Slide the two modules together (Refer to Figure 2-11).
Align the eight holes on the upper and side case panels,
and secure with screws.
Note
Use care to avoid damaging internal tubing and cables. Do not
pinch or crimp tubing or cables between modules.
2-7
BioOptix™ 10
Section 2 Installation
Figure 2-11 Aligning the fasteners
Figure 2-12 Column clamps inserted
2-8
BioOptix™ 10
Section 2 Installation
12. Ensure the Power Switch is in the Off (“0”) position. Then,
connect the AC power cord to the back of the Pump Module. Connect the other end of the power cord to an outlet
meeting the electrical requirements listed on the serial
number label.
13. Locate the Serial “Y” cable, P/N 60-1020-241 in the Accessories Kit. Connect the 25-pin connector labeled First
Instrument Optix to the External Control port on the back of
the Pump Module.
The BioOptix 10 may now be positioned on the bench as
shown in Figure 2-1.
WARNING
The system is heavy. Use a two-person lift to prevent injury.
14. Insert the ten column clamps into the BioOptix 10. Secure
the clamps by tighten the thumbscrews against the stainless steel shafts (Figure 2-12).
2.2.4 Assembling the Foxy 200
Drop former height
thumbscrews
10-channel drop
former assembly
1. Refer to section 2 of the Foxy 200 X-Y Fraction Collector
instruction manual. Unpack the Foxy 200 and assemble
the arm.
Note
To prevent damage to the ground wire on the fraction collector
arm, attach the ten-channel drop former to the arm before proceeding with the arm assembly instructions.
2. Loosen the two thumbscrews and raise the ten-channel
drop former assembly to its highest position (Figure 2-13).
Retighten the thumbscrews.
Figure 2-13 Foxy arm
3. Load collection tubes into the racks. Place the racks on the
Foxy 200.
4. Loosen the two thumbscrews and lower the ten-channel
drop former assembly so that it will clear the collection
tubes (Figure 2-13). Retighten the thumbscrews.
2-9
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