OPERATING
INSTRUCTIONS
Cryostat
TN50
INS2000GB
2018-10-003.00
Operating Instructions – TN50
2
CONTENTS
1. INTENDED USE ............................................................ 4
2. SYMBOLS ............................................................ 4
3. SAFETY NOTES ............................................................ 4
4. COMPONENTS ............................................................ 8
5. SPECIFICATIONS ............................................................ 9
6. UNPACKING AND INSTALLATION ............................................................ 11
7. INITIAL OPERATION ............................................................ 14
8. OPERATION OF MICROTOME ............................................................ 20
9. OPERATION OF CRYOSTAT ............................................................ 28
10. CLEANING AND MAINTENANCE ............................................................ 33
11. SERVICE ............................................................ 37
12. OPTIONAL ACCESSORIES ............................................................ 38
13. WARRANTY ............................................................ 39
14. DISPOSAL ............................................................ 39
Operating Instructions – TN50
3
1. INTENDED USE
The floor standing open top cryostat TN50 is intended for rapid freezing and cutting
thin sections of frozen specimens for professional use in routine and research
laboratories in the fields of biology, medicine and industry.
The system of knife advance operates very reliable from 0.5 to 100 µm with trimming
function up to 750 µm. The quality of cutting of this microtome is increased by the
automatic retraction during the upstroke of the specimen, which avoids rubbing on the
disposable blades or microtome knives. This stops rapid deterioration of the disposable
blade.
2. SYMBOLS
Dangers, warnings and cautions are marked by this symbol
Special instructions regarding the operation of the
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instrument are marked by this symbol
Mechanical components that can lead to injuries during
operation are marked with this symbol.
3. SAFETY NOTES
The Tanner Scientific open top cryostat TN50 is provided with the following safety
features:
TN50
Hand wheel stop
Finger protection for Blade-holder
The institution which owns the unit and the persons working with the unit, servicing or
repairing it are responsibe for a hazard-free use.
Operating Instructions – TN50
4
3.1 Hand wheel stop
Always use the finger protection
with the blade holder and put hand
wheel in stop position
before working with the
blade or specimen
before changing the
specimen
during break time
The hand wheel can be stopped in
any position. For this purpose move
the stop lever towards the centre.
To release the stop please turn the
lever towards the outside again.
Hand wheel stop released. Movement.
Hand wheel stop activated. No movement.
Operating Instructions – TN50
5
3.2 FINGER PROTECTION
Always use the finger protection (red
lever)
Before you start any work with
the blade or specimen
Before changing the specimen
During work break
Finger protection released.
Finger protection activated.
Operating Instructions – TN50
6
3.3 ELECTRICAL POWER CONNECTION
supply is identical
with the name plate of the unit
Do not use any extension lead.
Make sure that electric power is constant. Make sure that electric power
is constant. Please note that the compressor requires a start-up current
between 25 and 35 A. The electric circuit at the installation site must be
inspected by an electrical engineer to ensure that it meets the
requirements for a smooth operation of the instrument.
This should be examined during installation of the unit by a
competent person
Use a dedicated fuse for the unit
Do not connect another unit to the same power circuit.
Before turning on the instrument, check if the voltage of the mains
3.4 WORKING WITH DISPOSABLE BLADES
Disposable blades have extremely sharp edges and this can lead to injuries. Please be
extremely careful when handling disposable blades.
Do not place disposable blades at unsecured areas.
Never position disposable blades with the sharp edge pointing upwards.
Use a container that has guides to hold the blades rigid.
Never try to catch a falling disposable blade.
Always insert the specimen first and then the disposable blade.
To avoid compression or knife marks, ensure that the blade is clean.
Operating Instructions – TN50
7
4. COMPONENTS
The Tanner Scientific open top cryostat TN50 is provided with the following standard
components:
Basic unit with refrigeration system
Semi-automatic rotary microtome
Operation manual
clear-cut Brush
Anti-roll plate
Ready to use
1x Bottle cryostat low temperature oil
4x 125 ml CryoGlue embedding medium
TN50
1 Box of disposable blades (50 pcs.)
Set of Allen keys
Operating Instructions – TN50
8
5. SPECIFICATIONS TN50
General
Nominal power 115 V AC +-10%
Nominal frequence 60 Hz
Power 860 VA
Max. current for 5 sec 35 A
Protective class I
Fuses 2 * T 20 A
Pollution class 2
Overcurrent protective class II
Max. heat energy 860 J/s
Temperature range during operation +10 to +25 °C
Temperature during storage +5 to +55 °C
Relative humidity max. 60%, non condensing
Humidity during storage < 60%
Cryochamber
Temperature range 0°C to -35°C, selectable in 1 K steps,
Environment: 20°C
Cooling power 514 W
Switch off pressure 25 bar
Current 6,45 A
Cooling gas R 449 A, 320 g
Chamber defrost
Automatic defrost hot gas defrost, time selectable, 1-3 times per
day or manual defrost on request
Automatic stop at -5 °C chamber temperature
Freezing bar
Minimal temperature 10 K lower than chamber temperature
Number of positions 24 (standard version) resp. 21 + 2 (version
with quick freeze places)
Quick freeze positions 2 (optional), to -55 °C (Peltier cooling)
Operating Instructions – TN50
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Microtome
Type rotary microtome, incapsulated
Thickness range 0.5 – 100 µm
Thickness settings 0 – 2 µm in 0.5 µm steps
2 – 20 µm in 1 µm steps
20 – 50 µm in 2 µm steps
50 – 100 µm in 5 µm steps
Horizontal movement 28 mm
Vertical movement 58 mm
Retraction during upward stroke 0 – 200 µm, free selectable (defined steps)
Trimming 0 – 750 µm, free selectable (defined steps)
Specimen orientation 8° (x and y axes), 360° (z axes)
Cryostat
Dimensions (L x W x H) 680 mm x 760 mm x 1110 mm
27” x 30” x 44”
Weight (without accessories) 127 kg / 280 lbs
Operating Instructions – TN50
10
6. UNPACKING AND INSTALLATION
6.1 UNPACKING THE INSTRUMENT
In standard design you will receive the unit in a cardboard box standing on a wooden
pallet. The unit is equipped with four castors and two feet, adjustable in height in the
front.
When the instrument is delivered, check the tilt indicators and shock
watches on the packaging. If tilt indicators are activated, the shipment
was transported laying flat, was tilted at too great an angle or fell over
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Open the cardboard box from the top and remove the accessories together with the
supporting foams. Then remove the cardboard box.
Remove the screws on the two fixation brackets that hold the instrument on the
wooden pallet.
during transport. If the shock watches are activated, the instrument fell
over during transport. Note this on the shipping documents and check
the shipment for possible damage.
Swing out the ramp on which the instrument can be rolled down off the transport
pallet. To ensure a safe transport two (2) people are required.
Carefully roll the instrument backwards over the ramp from the pallet and push the
instrument to the installation location on the castors
Extend the two front feet to stabilize the cryostat in its position by turning the
supporting screws counter clockwise.
For repacking use the original cases. Keep the packing material.
Operating Instructions – TN50
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6.2 INSTALLATION SITE REQUIREMENTS
The site for installation for TN50 should meet the following requirements:
A maximum ambient temperature of 22°C
No direct sunlight into the cooling chamber
Mains power supply within 3 m
No air circulation (for example by air conditioning)
Instrument may only be used indoor
Main switch / fuses must be reached easily
Hand wheel must be reached easily
Relative humidity lower than 60%
A minimum distance between wall and rear of the instrument of 10-20 cm
(8 – 10 “ inches) should be guaranteed
High room temperatures and high humidity influence the cooling
capacity of the instrument and will lead to ice forming inside the
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6.2 INSTALLATION
For opening the chamber push the window to the top.
Remove the transport security underneath the specimen holder and position the waste
tray.
After installation, wait at least 4 hours before switching on the instrument. Failure to
comply may result in severe damage to the instrument.
instrument.
Operating Instructions – TN50
12
6.3 COLLAPSIBLE HANDWHEEL LEVER
The instrument is delivered with a
ready to use hand wheel lever
that is folded away for transport
reasons.
Before you start any work,
unfold the hand wheel
lever by simply pulling the
lever into a horizontal
position.
For folding the lever back,
pull the horizontally
orientated lever and fold it
away.
Hand wheel lever in folded position.
Hand wheel lever unfolded.
Operating Instructions – TN50
13
7. INITIAL OPERATION
CUT: 7 µm
Trim
: 19µm
7.1 MAIN DISPLAY
The main display will show the cutting thickness, the
trimming thickness, the current chamber temperature,
the target chamber temperature, the status of the
quick-freeze unit (optional).
7.2 CONTROL PANEL
Software settings and motor operation can be
operated with the control panel on the left to side of
the instrument.
Cha: -20 °C (-22)
QF: 0
TN50 Control Panel.
Operating Instructions – TN50
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7.3 SOFTWARE SETTINGS
CUT: 7 µm
Trim: 19µm
Press to open the Settings menu.
To move further down in the main menu list,
press .
To move further up in the main menu list,
Press .
To change values, select a setting.
Press to open the setting.
Change entries by pressing or .
Store new settings by pressing .
Chamber temp -20 °C
>
Quickfreeze Off
UV disinfection
Light Auto
Retraction 5 µm
Start defrost
Contrast
TIME TUE 13:05:05
>
Light Auto
>
Light duration 10 m
Chamber incr -15 °C
Retraction 5 µm
Light duration 10 m
Chamber incr -15 °C
>
Retraction 5 µm
Start defrost
Press to return to the main menu.
Cha: 22 °C (25)
QF: 0
Operating Instructions – TN50
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7.4 AUTOMATIC DEFROST, DISINFECTION AND POWER SAVING SETTINGS
_ _ _ _ _ 5
_ _ _ _ _ 5 6
Chamber temp -20 °C
>
Press to open the Settings menu.
Quickfreeze Off
UV disinfection
Light Auto
TIME TUE 13:05:05
Select Program settings and press to open the
menu for setting automatic defrost and power saving
times.
Language English
Display version
Program settings 5 µm
>
Enter PIN Code 5792.
PIN Code is usually restricted to service technicians or
instrument administrators
Enter PIN
0000
Select defrosting programs 1 to 3 and set a time for
the automatic defrosting programme.
(e.g. each day at 12:00 h and at 23:00 h in this
example)
Select temperature increase start and set a starting
time for the automatic power saving mode.
(e.g. start at 21:00 h in this example)
Select temperature increase end and set a ending
time for the automatic power saving mode.
(e.g. end at 04:00 h in this example)
Defr. Start 1 12:00
>
Defr. Start 2 23:00
Defr. Start 3 __:__
Disinf. Req. __:__
Disinf. delay 10 h
Incr. start 21:00
>
Incr. end 04:00
Incr. days
Disinf. delay 10 h
Incr. start 21:00
Incr. end 04:00
>
Incr. days
Operating Instructions – TN50
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Disinf. delay 1 2 _ _ _ _ _
Select temperature increase days and set the days at
that the automatic power saving mode will be active
for 24 hours.
Incr. start 21:00
Incr. end 04:00
Incr. days _ _ _ _ 5 6 _
>
(e.g. Fridays and Saturdays in this example)
1-Monday 2-Tuesday 3-Wednesday 4-Thursday 5-Friday 6-Saturday 7-Sunday
Operating Instructions – TN50
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7.5 List of available software settings
Chamber temperature
Object cooling
Object temperature
Quick-freeze
-35 °C to ambient
The recommended working temperature is -15 °C to -25 °C
ON / OFF
If the instrument is equipped with the optional
object cooling system, its function can be activated
or deactivated.
-50 °C to 0 °C
If the instrument is equipped with the optional
object cooling system, its function can be activated
or deactivated.
ON / OFF
If the instrument is equipped with the optional fast
cooling peltier system, its function can be activated
or deactivated.
UV disinfection
Light
The default setting for automatic deactivation of the
quick freeze is set to 30 minutes. The timer for
automatic deactivation can be set by an authorized
service technician.
ON /OFF
If the instrument is equipped with the optional UVC disinfection system, its function can be activated
or deactivated.
The default setting for automatic deactivation is set
to 30 minutes. The timer can be set by an
authorized service technician.
ON / OFF / AUTO
The light can be set on or off. If AUTO has been
selected, the light will switch off after the time
being set under Light duration.
Light duration
Operating Instructions – TN50
0 - 99 minutes
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Chamber Increase
For power saving, a higher temperature can be set
for defined times and/or days.
The selected temperature should be below 0 °C to avoid
damage to the microtome by freezing moisture.
Retraction
Start defrost
Trim latching
Contrast
Time
Language
0 - 200 µm
Activate
A manual defrost procedure is activated.
ON/OFF
If Trim latching mode is activated, trimming function
is activated and deactivated by pressing once.
If Trim latching mode is deactivated, trimming
function is activated by continuously pressing .
Display contrast
Day and Time
A language can be selected out of a list of available
languages.
Display version
Program settings
Display of software version.
Upon selecting Program settings the automatic
defrost and power saving settings can be changed.
Operating Instructions – TN50
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8. OPERATION OF MICROTOME
2 4
3
8.1 INSERTION AND ORIENTATION OF DISPOSABLE BLADE
1 Activate hand wheel lock.
2 Remove finger protection.
3 Loosen the blade fixation by
turning the right lever counter
clockwise.
4 Remove/Insert blade from one
side.
Tighten the blade fixation by
turning the right lever clockwise.
Loosening of blade fixation and insertion of new blade.
Fixation of blade.
Operating Instructions – TN50
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For adjusting the cutting angle of
the blade, loosen the blade holder
fixation by turning the right lever on
the blade holder base counter
clockwise.
Set new angle for cutting by turning
the knob in the front of the blade
holder or by manual tilting the blade
holder. A tilt towards the object
holder increases the cutting angle
(clockwise turn of the knob), a tilt
away from the object holder
decreases the cutting angle (counter
clockwise turn of the knob).
Loosening of blade holder fixation and adjusting new
cutting angle (increased cutting angle).
Tighten the blade holder fixation by
turning the right lever on the blade
holder base clockwise.
Release hand wheel lock for cutting.
Fixation of blade holder with increased cutting angle.
Operating Instructions – TN50
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8.2 SPECIMEN MOUNTING
1 2 4
3
The instrument is equipped with 24
freezing positions. The temperature
is approx. 10 °C lower than the
actual cryochamber temperature.
In order to prepare a specimen for
sectioning, place the specimen
holder on a freeze point, add some
drops of embedding medium
(e.g.GSV1) on the holder and
carefully press the object on top.
Wait until the object is frozen (mat
surface or pressure).
8.3 INSERTION OF OBJECT HOLDER
1 Turn hand wheel and move
object holder to its highest
position and activate the hand
wheel stop.
2 Release the object holder
fixation by turning the fixation
lever counter clockwise.
3 Remove/Insert object holder.
4 Tighten the object holder by
turning the fixation lever
clockwise.
Please pay attention, that under
all circumstances during
operation, the fixation lever must
not point downward, otherwise
there is a risk of collision and
damage.
Release of object holder fixation; removal and/or insertion of
object holder.
Operating Instructions – TN50
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8.4 SPECIMEN ORIENTATION ADJUSTMENT
4
1
Turn hand wheel to its highest
position and activate the hand wheel
stop.
1. For a new sample orientation,
loosen the fixation lever on the
left side of the sample holder
counterclockwise.
2. To adjust the orientation,
swing the right orientation
screw.
2
Fix the sample orientation by turning
the fixation lever on the left side of
the sample holder clockwise.
Release the handwheel lock to cut the
samples.
Release the sample orientation and
orientation of the sample.
Fix the sample orientation.
Levers must be up!
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Operating Instructions – TN50
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8.5 FAST APPROACH TO SPECIMEN
--
TRIM
-- Trim: 19µm
Press to move the knife holder base towards the sample.
Press to move the knife holder away from the sample.
8.6 SLOW APPROACH TO SPECIMEN
Press to move the knife holder base slowly towards the sample.
8.7 THICKNESS ADJUSTMENT FOR TRIMMING
To change the trimming settings, press continuously and press or to increase
or decrease the value.
8.8 THICKNESS ADJUSTMENT FOR CUT
To change the cutting thickness, press or to increase or decrease the value.
8.9 TRIMMING OF SPECIMEN
Insert object holder.
Orientate specimen as desired.
Deactivate the hand wheel stop.
Release finger protection.
If Trim latching mode is activated, trimming function is
activated and deactivated by pressing once.
Cha: -20 °C (-22)
QF: 0
Activation of trimming function is displayed as
illustrated.
If Trim latching mode is deactivated, trimming function is activated by continuously
pressing .
Activation of trimming function is displayed as illustrated above.
Turn hand wheel evenly clockwise
Operating Instructions – TN50
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8.10 MANUAL CUTTING
CUT: 7 µm
Trim: 19µm
Deactivate the hand wheel stop.
Release the finger protection.
Turn hand wheel evenly clockwise.
A counter clockwise movement will also result in cutting and incremental advance of the specimen.
Approach specimen either by trimming or slow approach.
Set the required section thickness. Change entries by
pressing or . The selected section thickness is
indicated in the display.(e.g. 7 µm in this example)
Turn hand wheel until some even sections have been obtained.
Clean the disposable blade (always away from the cutting edge) with a cold brush.
Position anti-roll plate onto the disposable blade and cut. If necessary readjust the
height of the guide plate.
Leave hand wheel with handle at its lowest point (specimen holder underneath the
disposable blade).
Transfer the section onto a slide.
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Leave brush always in the chamber to keep it cold.
Cha: -20 °C (-22)
QF: 0
Operating Instructions – TN50
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8.11 ANTI-ROLL PLATE
Each instrument is equipped with
an anti-roll plate.
To adjust the horizontal position
of the anti-roll plate, turn the
wheel to the left (towards blade)
or right (away from blade).
For pick up of the samples, turn
the anti-roll plate sidewards with
the help of the knob.
In this case, make sure that the
anti-roll-plate is folded into the
horizontal position when it is
unfolded in order to prevent a
one-sided warm-up.
Horizontal adjustment of anti-roll plate
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For height adjustment of the anti-roll-plate, it is recommended to
adjust the correct height during cutting.
Operating Instructions – TN50
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8.12 TIPS AND TRICKS
spray can be used.
It is common knowledge that the temperature of the cryostat plays an important role in
the quality of the tissue sections obtained. Each type of tissue needs a different
temperature as shown in the following list. The temperature ranges indicated in the
table below are approximate values and may require adjustments for individual tissues.
Type of Tissue
Bone marrow -16 to -25
Brain -7 to -10
Breast with fat -25 to -30
Breast without fat -16 to -20
Cartilage -13 to -20
Fat -30 to -40
Heart -20 to -25
Intestinal -13 to -20
Kidney -13 to -20
Larynx -13 to -16
Lip -10 to -20
Liver -7 to -13
Lung -13 to -20
Recommended temperature range at
the blade edge [°C]
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For tissue which has to
be cut considerably
below normal chamber
temperature (e.g. fatty
tissues) and in order to
avoid cooling artefacts,
an additional bench top
CO2 freezer is available.
Exceptionally freezing
Lymph-Node -13 to -20
Lymphatic -13 to -20
Lymphoid -13 to -20
Muscle -13 to -20
Nose -13 to -20
Rectal -13 to -20
Scrapings -16 to -25
Skin with fat -16 to -25
Skin without fat -10 to -16
Spleen -7 to -10
Testicle -10 to -13
Tongue -13 to -20
Uterine- -7 to -10
Operating Instructions – TN50
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9. OPERATION OF CRYOSTAT
9.1 UVC DISINFECTION [only applicable to instruments equipped with this feature]
UV-C radiation at 254 nm has an intense germicidal effect. Microorganisms, such as
viruses, bacteria, fungi and yeasts are effectively destroyed in the area accessible to UV
light without the addition of chemicals.
The cryostat TN50 can be equipped with a UVC light source that can either request
activation in defined periods (see section 7.3) or is manually activated.
The default setting for the UVC light duration and the automatic deactivation is set to
30 minutes. The timer can be set by an authorized service technician.
For safety reasons UVC light will be deactivated upon opening of the
lid of the cryochamber. The UVC disinfection will be aborted and
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has to be activated again for surface disinfection.
The default setting for automatic deactivation is set to 30 minutes.
The timer can be set by an authorized service technician.
9.1.1 INSTALLATION INSTRUCTION FOR UV LAMP - CRYOSTAT
Please follow the respective steps and pay attention to the relevant instructions.
Never directly touch the UV lamp´s glass, since this reduces its durability.
Picture 1:
Operating Instructions – TN50
28
Picture 2:
Picture 3:
Slide the UV lamp as shown into the glass bulb. Keep 2 cm space before the
endposition will be reached, this place will be needed to connect the UV-lamp with
the connecting plug. (Pic. 2 & 3)
Picture 4:
Picture 5:
The connector can be plugged into two
directions of polarity, a switch is
excluded.
Picture 6:
Picture 7:
Once the plug is connected to the UV-lamp, the final step can be done now and the
UV-lamp unit can clip into the retaining clips.
Operating Instructions – TN50
29
9.2 QUICK FREEZE POSITION [only applicable to instruments equipped with this feature]
For convenience of the user, the cryostat TN50 can be equipped with a quick freeze
shelf with two positions located in the object freezing shelf. Upon activation the
installed peltier element will cool down the two quick freeze position to approximately 55 °C.
The quick freeze shelf can be activated as described in section 7.2.
The default setting for automatic deactivation of the quick freeze is
set to 30 minutes. The timer for automatic deactivation can be set
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by an authorized service technician.
9.3 HEAT EXTRACTOR
For a more rapid freezing process with
improved structural preservation of tissues
and a flattened block surface, the
instrument is equipped with a heat
extractor.
Apply the heat extractor on top of the
specimen during the freezing process.
Use of the heat extractor can lead to changed orientation of the
specimen in the frozen block, especially for smaller samples.
i
If precise orientation of the specimen is required, the freezing
procedure should be performed without the heat extractor.
Heat extractor
Operating Instructions – TN50
30
9.4 AUTOMATIC/MANUAL DEFROST
For an optimal effect of the cooling machine, a low thermal resistance at the cooling
ribs is necessary. Frequent use of the cooling machine condenses moisture on these
cooling ribs and increases the thermal resistance. Therefore, the device, in particular the
cooling ribs, is automatically defrosted.
During an automatic defrost (programmed for every midnight) or an manual activated
defrost (possible at every time) hot gas from the cooling system is pressed through the
evaporator. All ice which has been built up is melted, the water drops into the
condensate bottle.
The defrosting process ends at a temperature of 20 °C on the evaporator. The chamber
temperature remains in the minus range, so that samples which are stored there do not
thaw.
The defrost process starts automatically at midnight every day. To
i
change this time, please refer to section 7.3.
Before starting a complete defrost of the instrument, assure that all
samples have been removed from the cryochamber.
i
Before switching on the instrument ensure that the instrument is
completely dry, otherwise ice built up can damage mechanical parts
During defrosting (automatic or manual) always leave front
window open.
Operating Instructions – TN50
31
9.5 COMPLETE DEFROST OF THE INSTRUMENT
It is highly recommended to defrost the instrument at least every 6 months. If the
instrument is used regularly in warm tropical climate a more frequent complete defrost
might be necessary. The unit should be defrost for a minimum period of 48 h, better
over the weekend.
Turn hand wheel to its highest position and activate the hand wheel stop.
Remove samples and tools from the cryochamber.
Switch off the instrument. Be sure to open the front window and leave it open until it is
switched back on.
Before restarting, the microtome and the cooling chamber must be completely dry in
order to avoid a decision.
Switch on the instrument.
Before starting a complete defrost of the instrument, assure that all
samples have been removed from the cryochamber.
i
Before switching on the instrument ensure that the instrument is
completely dry, otherwise ice built up can damage mechanical parts.
9.6 EMPTYING THE CONDENSATE BOTTLE
The unit is equipped with a condensat bottle for defrosting water. The filling level of the
collecting container is checked with a fill level sensor. As soon as "full container" is
displayed in the display, the collecting container must be emptied in order to avoid
overfilling.
The contents of the condensate bottle should be disposed according to
i
laboratory regulations.
To reduce the risk of contamination and infection, after emptying, is
recommended to fill a disinfectant concentrate into the condensate
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bottle..
Operating Instructions – TN50
32
10. CLEANING AND MAINTENANCE
10.1 CLEANING
The recommended frequency of cleaning of the cryostat chamber depends on how
frequently the instrument is used.
Wear protective clothing and disposable gloves according to Good
Laboratory Practices.
Please take note of the safety aspects of the instrument.
Do not use acetone or xylene for cleaning the unit. Only use alcoholic
media.
Never spray or use cleaning medium directly onto the touch panels.
10.2 DISINFECTION
Spray disinfection with alcohol-based disinfectants is applicable without removing the
microtome from the cryochamber. When disinfecting the instrument, take appropriate
protective measures (gloves, mask, protective clothing, etc.).
Wear protective clothing and disposable gloves according to Good
Laboratory Practices.
When using detergents and disinfectants please comply with the safety
precautions of the disinfectant manufacturer!
The integrated anti-roll plate of the blade holder can be cleaned with
alcohol.
Dispose of waste liquid according to the waste disposal regulations!
Do not turn the instrument on before the cryochamber and the
microtome are completely dry otherwise ice built up can damage
mechanical parts.
All components removed from the cryostat must be carefully dried before
returning them to the cryochamber!
Operating Instructions – TN50
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10.3 RECOMMENDED MAINTENANCE AND SERVICE SCHEDULE
Daily
Weekly
Monthly
Yearly
Remove frozen section waste from the cryostat chamber after every day
of usage.
Disinfection if necessary.
Cleaning of blade holder (see section 10.3).
Cleaning of window.
Check of temperature sensor in cryochamber.
Cleaning of cryostat ventilation (see section 10.4).
Complete Service (performed by authorized Tanner Scientific service technician)
check of all functions
complete defrost
removing of microtome
oiling of movable parts
check of cooling system
check of driving system
complete disinfection / cleaning / drying
Operating Instructions – TN50
34
10.4 CLEANING OF DISPOSABLE BLADE HOLDER
Turn the handwheel to the highest
position and operate the handwheel lock.
Loosen the blade by turning the lever
counterclockwise.
Remove the blade from the blade holder
using the ejection mechanism. Loosen the
upper part of the blade holder by turning
the upper lever counterclockwise. Slide
the top of the blade holder to the right.
Remove the pressure plate and clean the
blade holder with a brush or cloth
Pull out the lever and release the upper blade
holder.
Remove the pressure plate
Reassemble the pressure plate and the
lever in reverse order and release the
handwheel lock for cutting.
Operating Instructions – TN50
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10.5 CLEANING OF CRYOSTAT VENTILATION
During usage of the instrument,
dust will deposit on the ventilation
of the cooling unit. This can
negatively affect the cooling
performance of the instrument.
A cleaning of the cryostat
ventilation is recommended
monthly.
Remove the front panel of the
unit by unscrewing the 8 screws
(four on each side). Remove the
dust with a vacuum cleaner.
A cleaning of the cryostat
ventilation is recommend-ded
monthly. We suggest using a
vacuum cleaner and a brush for
cleaning.
Operating Instructions – TN50
36
11. SERVICE
Internal components should only be serviced by technicians authorized by Tanner
Scientific.
If technical service or spare parts are necessary, please contact your local Tanner
Scientific distributor. Please have the following information available:
Complete contact details
Type of instrument and serial number
Place of instrument and name of user
Purpose of service call
Delivery date of the unit
If it is necessary to return the instrument, it must be cleaned and disinfected before
delivery. It must be returned in its original packing.
If the instrument or parts thereof are sent back in a dirty or non-disinfected condition,
Tanner Scientific reserves the right to return the parts to the debit of the customer.
Operating Instructions – TN50
37
12. OPTIONAL ACCESSORIES
Disposable Blade Holder (low profile)
Disposable Blade Holder (high profile)
2x Quick freezing positions
UV disinfection
Heat extractor block
Cork plate (100 pcs.; Ø 20 mm)
clear-cut Brush
Anti-roll plate (disposable blade holder)
Operating Instructions – TN50
38
13. WARRANTY
Tanner Scientific guarantees that the product delivered has been subjected to a
comprehensive quality control procedure, and that the product is faultless and complies
with all technical specifications and/or agreed characteristics warranted.
Tanner Scientific guarantees that the instrument is manufactured under an ISO 9001
quality management system.
Unauthorized modification or repair by third party persons will void the warranty.
Only original Tanner Scientific spare parts must be used.
Guarantee claims can be put forward only if the instrument is used according to this
manual and for the purpose described.
Mistakes and errors which occur because of improper use cannot be accepted.
14. DISPOSAL
The instrument or parts of the instrument must be disposed of according to existing
local applicable regulations.
Operating Instructions – TN50
39
Tanner Scie
ntific
12210 Rangeland Parkway
34211 Bradenton FL / US
Tel. (888) 708-5233
sales@tannerscientific.com
http://www.tannerscientific.com
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