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Page 1 of 33
Takara Bio USA, Inc.
Apollo™ System User
Manual
Cat. No. 640078
(080719)
Takara Bio USA, Inc.
1290 Terra Bella Avenue, Mountain View, CA 94043, USA
U.S. Technical Support: technical_support@takarabio.com
+1.650.919.7300
+33.(0)1.3904.6880
+81.(0)77.565.6999
Apollo™ System User Manual
Table of Contents
I. Introduction ..................................................................................................................................................................... 4
II. List of Components ......................................................................................................................................................... 4
III. Safety Information ...................................................................................................................................................... 5
A. Symbols ....................................................................................................................................................................... 5
B. Installation and Storage ............................................................................................................................................... 6
C. Precautions for Instrument Operation ......................................................................................................................... 6
D. Warranty Period .......................................................................................................................................................... 6
E. Abolishment ................................................................................................................................................................ 6
F. Actions in Case of Emergency .................................................................................................................................... 7
IV. Specifications .............................................................................................................................................................. 7
A. Features ....................................................................................................................................................................... 7
B. Performance Specifications ......................................................................................................................................... 8
C. Size, Weight and Power Requirements ....................................................................................................................... 8
D. Environmental Requirements ...................................................................................................................................... 8
E. About Caution Labels Inside the Instrument .............................................................................................................. 9
V. System Components ...................................................................................................................................................... 10
A. Front View ................................................................................................................................................................ 10
B. Right-side View ........................................................................................................................................................ 11
C. Internal Components ................................................................................................................................................. 12
VI. Installation ................................................................................................................................................................. 13
A. Setup Procedure ........................................................................................................................................................ 13
VII. Accessories and Disposables .................................................................................................................................... 16
A. Accessories ............................................................................................................................................................... 16
B. Disposables ............................................................................................................................................................... 18
C. Reagents .................................................................................................................................................................... 19
VIII. Directions for Use ..................................................................................................................................................... 19
A. Front Door Operation ................................................................................................................................................ 19
B. Putting on and Taking out the Waste Tip Box .......................................................................................................... 20
C. Setting Racks and Disposables in Place .................................................................................................................... 21
IX. Basic Operation ......................................................................................................................................................... 23
A. Start Up and Shut Down Procedure .......................................................................................................................... 23
B. Touch Panel Operation .............................................................................................................................................. 23
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C. Running a GUI Protocol ........................................................................................................................................... 24
D. Running a User Maintenance Protocol ..................................................................................................................... 25
E. About Page ................................................................................................................................................................ 26
X. Instrument Care and Maintenance ................................................................................................................................ 26
A. Cleaning Procedure ................................................................................................................................................... 27
B. Greasing O-rings ....................................................................................................................................................... 27
XI. Troubleshooting ........................................................................................................................................................ 29
A. Error Reported During Protocol Run ........................................................................................................................ 29
B. Suspending or Aborting a Protocol Run ................................................................................................................... 29
C. Frequently Reported Problems .................................................................................................................................. 30
XII. List of Common Error Codes .................................................................................................................................... 31
Appendix ............................................................................................................................................................................... 32
A. Recovering a Run After a Crash ............................................................................................................................... 32
B. Resetting the Apollo system axes after a crash ......................................................................................................... 32
Table of Figures
Figure 1. Requirements for proper placement of the Apollo system ...................................................................................... 9
Figure 2. Front view of the Apollo system. .......................................................................................................................... 10
Figure 3. Right-side view of the instrument showing the position of Power Inlet Connector and the Fuse Unit. ................ 11
Figure 4. Location of the Magtration Unit and the Stage Unit inside the instrument. .......................................................... 12
Figure 5. Image showing the location of the disposables on the Stage Unit. ........................................................................ 13
Figure 6. Image showing the operation of the Front Door. ................................................................................................... 20
Table of Tables
Table I. List of components provided with the Apollo system. .............................................................................................. 4
Table II. List of accessories provided with the instrument. .................................................................................................. 16
Table III. Disposables available from Takara Bio ................................................................................................................ 18
Table IV. Maintenance checklist .......................................................................................................................................... 26
Table V. Frequently reported problems ................................................................................................................................ 30
Table VI. List of error codes ................................................................................................................................................. 31
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Apollo™ System User Manual
No.
Description
Qty.
Remarks
1
Apollo System Main Body
1 2
Front Door Handle
1
With 2 fixing screws
3
96 Tip Rack
3 4
Reagent Reservoir Rack
1 5
Mini-Tube Rack
1
For 1.1 ml tubes
6
PCR Tube Retention Plates
2 7
Waste Tip Box
1
Content
8
Waste Chute
1 9
Stylus Pen
1 10
Stylus Pen Holder
1 11
AC240V Power Cable
1 12
AC110V Power Cable
1 13
O-rings
8 14
Silicon Grease
1
For O-rings
15
Fuse
1 16
User Manual
1
This manual
17
Package Checklist
1
I. Introduction
We appreciate your purchase of the Apollo system, which utilizes Magtration® technology for separation of
magnetic particles. This manual is a guide for the user to operate the instrument safely and effectively. Please read
this manual thoroughly to understand the instrument before starting operation. Keep this manual close to the
instrument as a quick reference for all the operators as needed.
• Do not operate the instrument without fully understanding instructions described in this manual.
• Always keep this manual handy for easy reference at any time.
• Please inform us if this manual is lost or damaged.
II. List of Components
Table I. List of components provided with the Apollo system.
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This symbol indicates the possibility that one could be killed or seriously injured when
This symbol indicates the possibility that one could be injured when an incorrect
Denotes information to be emphasized or added for important points. You are advised
This symbol indicates caution against electric shock.
This circled symbol indicates actions that are prohibited. Details are illustrated inside
The closed circle symbol is used to restrict operations or to illustrate what to do.
The following precautionary instructions are included to ensure the safe operation of
III. Safety Information
Most problems occur from improper use of the instrument and when safety instructions are not followed. Please
read this manual thoroughly to understand and to get familiar with preventive measures so any problems in
instrument operation can be avoided.
A. Symbols
In this manual we put symbols next to important instructions regarding safe use of the instrument. Details
of all symbols are provided below.
an incorrect operation is performed.
operation is performed.
to pay close attention to these when operating the instrument.
or next to the symbol.
Details are illustrated inside the symbol (the sample on the left shows unplugging of
the AC plug from a wall outlet).
your Apollo System. Please always keep this manual near the instrument or operator.
Failure to comply with the instructions in this manual will void the manufacturer’s
warranty. If the instrument is not performing as expected, immediately turn off the
main-power switch and unplug power cable, then contact us.
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• direct sunlight
Unplug power cable from wall outlet when
B. Installation and Storage
Avoid the following places for the installation of this instrument. Installing this instruments in such places
may cause damage to the instrument.
Do not install in a place with
• vibration, high humidity or dust
• strong electric or magnetic
fields
• possibility of liquid or oil
splashing on the instrument
• flammable or corrosive gas or
high heat
instrument is likely to not be in use for extended
periods of time.
C. Precautions for Instrument Operation
• To eliminate the possibility of biohazard contamination from samples or extracted/purified products,
use a mask, goggles and gloves while operating the instrument for your safety.
• Pay close attention when handling samples with high risk of biohazard.
• When operating the instrument under low temperature conditions, keep the instrument powered on
even when not in use.
• Be sure to avoid any foreign materials from getting into the instrument.
• Do not scratch or press the operation panel using knives or things with sharp edges.
• Replacement of the fuse shall be made by field service personnel authorized by Takara Bio. Please
contact us for fuse replacement.
D. Warranty Period
• 12 months after installation, regardless of use.
• This warranty excludes the problems derived from inadequate storage, improper use or operation,
repair/modification/maintenance of the instrument by people who are not authorized by us, even
within the warranty period.
E. Abolishment
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Contact us when you want to recycle the instrument.
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In the instance when the instrument gets extremely hot, is
When foreign material or water splashes over or gets inside the
When the power cable is damaged (core wire exposed or broken),
F. Actions in Case of Emergency
smoking, giving off an abnormal smell, etc., immediately unplug
the power cable from the wall outlet to avoid fire or electric shock
to you and contact us for repair.
instrument, immediately unplug the power cable from the wall
outlet to avoid fire or electric shock to you and contact us.
immediately unplug the power cable from the wall outlet to avoid
fire or electric shock to you and contact us.
IV. Specifications
The Apollo system provides a complete, walkaway solution for next-generation sequencing (NGS) library-prep
applications using validated protocols and chemistries. In addition to library prep, the Apollo system also employs
in-tip magnetic bead separation, for a variety of magnetic bead applications such as PCR clean up, poly A
enrichment, rRNA depletion, and size selection.
A. Features
• Simple operation using a graphical user interface
• Compact design with minimal installation footprint
• High availability of various types of magnetic particle reactions by using special protocol
development software
• Simultaneous processing of up to 8 samples by 8 nozzles (batch processing of up to 96 samples)
• Reduced running cost by using reagent dispensing function and by using commercially available
microtiter plates
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Instrument type:
Desktop
Throughput:
Samples: 1–96 samples/batch depending on protocol type
Processing time: about 30 min/ 1–8 samples
Temperature control:
Two Heat/Cool Blocks: 4–95°C
Handling volume:
5–200 µl
Dispensing accuracy*:
5–10 µl less than CV 20 %
11–20 µl less than CV 10%
21–200 µl less than CV 2%
B. Performance Specifications
* Accuracy was measured with distilled water at room temperature 20–25°C.
NOTE: Processing time depends on the protocol being performed.
C. Size, Weight and Power Requirements
Size: 800 mm (W) x 700 mm (D) x 675 mm (H) (disregarding prongs)
Weight: 97 kg (~214 lbs) (excluding accessories and disposables)
Power Required: AC100 – 240 V, 50/60 Hz, 440 VA
• Do not share power source with other instruments.
• Do not place close to noisy instruments or instruments with power fluctuation.
• Connect power cable to wall outlet with ground line.
D. Environmental Requirements
Temperature: 15°C–30°C
Relative Humidity: 15–75% Non-condensing
Altitude: Up to 2,000 m
Space: maintain a minimum distance between the walls and instrument as shown below for ventilation,
maintenance work, etc. Also keep more than 200 mm of space above the instrument.
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Apollo system
Figure 1. Requirements for proper placement of the Apollo system. The instrument should be placed such that there is a
distance of at least 200 mm between the instrument and any surrounding walls.
• Place the instrument on a horizontal surface.
• Do not place the instrument where there is direct sunlight or vibration.
• When operating the instrument in a cold chamber or cold room, keep the instrument powered
on even when not in use to avoid formation of dew.
E. About Caution Labels Inside the Instrument
1. Caution label for high temperature
Place attached: Heat/Cool Block
The part where the yellow label is attached becomes hot (see image above). Do not touch this area
while handling the instrument.
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2. Caution Label for Biohazard
Place attached: Waste Chute
The part where this label is attached (inside cover) has the possibility of biohazard contamination
with samples or extracted/purified products. Take care to avoid contamination.
V. System Components
A. Front View
Figure 2. Front view of the Apollo system.
NOTE: The Power Lamp lights up (green light) when the machine is powered on.
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B. Right-side View
On the right side of the instrument, the Power Switch is located towards the front and the Power Inlet
Connector and Fuse Unit are located towards the back.
Figure 3. Right-side view of the instrument showing the position of Power Inlet Connector and the Fuse Unit.
1. Details of the Right-side View (Front)
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2. Details of Right-side View (Back)
NOTE: Replacement of the fuse should be performed by field service personnel authorized by
Takara Bio. Contact us regarding fuse replacement.
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C. Internal Components
The Magtration Unit and the Stage Unit are located inside the instrument.
Figure 4. Location of the Magtration Unit and the Stage Unit inside the instrument.
Magtration Unit
Nozzle Unit: This unit has 8 nozzles to process up to 8 samples simultaneously with accurate and rapid
aspiration/dispensing.
Magnet: Eight powerful magnets are aligned with 8 tips to simultaneously separate magnetic particles for
up to 8 samples.
Stage Unit
Heat/Cool Unit: (indicated by the red box in Figure 4, above)
Two Heat/Cool Units are equipped and can hold PCR plates. Temperature can be set from 4 to 90°C
depending on protocols.
Rack Holder: (indicated by the green box in Figure 4, above)
Racks and Disposables to be used vary by protocols. Refer to the application manual of the protocol you
are following for details.
Waste Chute: It leads the waste tips and liquid to the Waste Tip Box.
Example of loading racks and disposables
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Figure 5. Image showing the location of the disposables on the Stage Unit.
96 Tip Rack: Holds 8 x 12 DN70 Tips.
Reagent Reservoir Rack: Holds Reagent Container L.
Microtiter Plates: Holds 96-well Microtiter plates.
Waste Chute: Place where waste tips, etc. are discarded.
NOTE: The above layout is an example and will vary by protocol.
VI. Installation
A. Setup Procedure
NOTE: Setup should be performed by Field support personnel authorized by Takara Bio.
1. Remove protection films attached on the instrument.
2. Open Front Door and fix Grip of Front Door by two screws (supplied).
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3. Remove the fixing jig for X-Axis Magtration Unit fixed by 3 screws inside the instrument.
4. Remove cushioning and desiccant agents.
5. Open Right-side Cover by removing 2 fixing screws.
6. Remove Y-Axis fixing jig to right-side base by removing 3 fixing screws. Then replace Right-side
Cover and fix it with two screws.
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7. Open Left-side Cover by removing 2 fixing screws.
8. Remove Y-Axis fixing jig to left-side base by removing 4 fixing screws. Then replace Left-side Cover
and fix it with two screws.
9. Connect Power Cable to Power Inlet Connector on the right side of the instrument. Then turn Power
Switch on assuring that Front Door and Waste Tip Box Door are closed securely.
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Description
Qty.
96 Tip Rack
3
Reagent Reservoir Rack
1
Mini-Tube Rack
1
PCR Tube Retention Plates
2
Waste Tip Box
1
Waste Chute
1
Stylus Pen
1
Stylus Pen Holder
1
AC240V Power Cable
1
AC110V Power Cable
1
O-ring
8
Silicon Grease
1
Fuse
1
Do not use power cable other than the one provided to avoid temporary blackout or fire breaking.
10. Installation Completed
When transferring the instrument, make sure that all the fixing jigs for all axes are attached
securely and that cushioning is set between the Magtration Unit and the Stage Unit to protect
nozzles from free movement.
VII. Accessories and Disposables
A. Accessories
Table II. List of accessories provided with the instrument.
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• 96-Tip Rack
Holds DN70 Tips, up to 8 x 12 pieces.
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• Reagent Reservoir Rack
Holds up to 4 Reagent Containers.
• Waste Tip Box
The Waste Tip Box receives discarded tips coming down through the Waste Chute.
• Waste Chute
The Waste Chute directs discarded tips to the Waste Tip Box.
• Stylus Pen
The Stylus Pen is used to operate the instrument through the Touch Panel. It operates like a ballpoint pen.
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Cat. No.
Product Description
640089
Apollo 96 Tip Rack
640085
Apollo Piercing Tips
640084
Apollo Filter Tips
640082
Apollo 0.2 ml PCR 8-Tube Strips, Clear
640086
Apollo Caps for 0.2 ml PCR 8-Tube Strips, Clear
640083
Apollo Microtiter Plates
640087
Apollo Reservoirs
640088
Apollo 1.1 mL MiniTubes
• Stylus Pen Holder
The Stylus Pen Holder is a holder for the Stylus Pen and can be magnetically attached onto the
instrument surface.
B. Disposables
1. Disposables available from Takara Bio
Table III. Disposables available from Takara Bio
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•
Apollo Reservoirs (Cat. No. 640087)
Apollo Filter Tips (Cat. No. 640084)
•
C. Reagents
Refer to your specific application manual for reagents to be used and directions for use.
• Do not reuse any disposables/reagents to avoid contamination or instrument malfunction.
• Do not use disposables/reagents other than specified to avoid instrument malfunction.
• When discarding disposables, follows the instructions defined by your facility or regional laws.
VIII. Directions for Use
A. Front Door Operation
The Front Door moves up and down, and it is held at the top when fully moved up. Hold only the
Grip/handle of the Front Door to open or close the door to avoid getting your fingers pinched. The Front
Door is locked during operation. Open the Front Door after the instrument has finished its operation.
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Figure 6. Image showing the operation of the Front Door.
• If the Front Door is not opened to its uppermost position, it may come down and your hand or
fingers might be pinched. Make sure to move the Front Door to its uppermost position when
working.
• Move Front Door gently without giving excessive force to avoid damaging it.
B. Putting on and Taking out the Waste Tip Box
1. Waste Chute
The Waste Chute can be removed by taking it out from Waste Tip Discard Hole for cleaning.
2. Waste Tip Box
The Waste Tip Box can be removed for discarding tips or for cleaning. Open the Front Door first and
then open the access door for the Waste Tip box. Take out the Waste Tip Box by holding its grip.
Make sure that the access door for the Waste Tip Box is closed after setting the Waste Tip Box,
otherwise you cannot start a protocol run.
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96 Tip Rack
Reagent Reservoir Rack
No positioning
Position the cut to Spring
Move the access door for the Waste Tip Box gently without excessive force to avoid damaging it.
C. Setting Racks and Disposables in Place
1. Setting Racks
Set racks on their designated position and make sure they are set securely.
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• When Racks are not correctly positioned, tip pickup failure, inaccurate
aspiration/dispensing, or instrument malfunction may occur.
• When Reagent Tip Racks are not correctly positioned, tips cannot be picked up resulting in
instrument malfunction.
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2. Setting Microtiter Plates
Set the Microtiter Plates in positions designated by the selected application, following positioning
directions.
• When Microtiter Plates are not positioned correctly, adequate aspiration/dispensing or
liquid mixing may not be performed, resulting in inadequate extraction/purification.
• If non-recommended microtiter plates are used, adequate aspiration/dispensing or
liquid mixing may not be performed resulting in inadequate extraction/purification.
3. Setting PCR Tubes onto the Heat/Cool Unit
Place the PCR Tubes on the Heat/Cool Unit by following the positioning directions and push them
down to fit securely.
• When the PCR Tubes are not positioned correctly, adequate aspiration/dispensing or liquid
mixing may not be performed, resulting in inadequate extraction/purification.
• If a non-recommended PCR Tubes are used, adequate aspiration/dispensing or liquid
mixing may not be performed, resulting in inadequate extraction/purification.
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IX. Basic Operation
A. Start Up and Shut Down Procedure
1. Start Up
1. Close the access door for the Waste Tip Box and the Front Door. (Refer to Section VIII.B,
“Putting on and Taking out the Waste Tip Box”, and Section VIII.A, “Front Door Operation”.)
2. Turn on the Power Switch located on the right side of the instrument.
3. The instrument starts up and the Touch Panel screen is activated.
2. Shut Down
1. Turn the instrument power off, making sure that the instrument is not operating*.
* If it is operating, stop operation by pressing the “Protocol Stop” button, and then turn off the
system.
B. Touch Panel Operation
Using the Stylus Pen, select the function desired.
• Operate the Touch Panel with the Stylus Pen gently without giving excessive force to avoid
damaging it.
• Use only the Stylus Pen provided to avoid damaging the Touch Panel screen with other tools
with sharp edges.
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C. Running a GUI Protocol
1. Select the desired operation from the MAIN screen.
2. For example, select Utility Apps to perform PCR Cleanup.
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3. Select the desired sample size PCR cleanup on the Utility Apps screen. For example, PCR Cleanup 8
for 8 samples.
4. Using the “Back” and “Next” buttons, follow on-screen block-by-block instructions.
5. Once the deck is set up and cooled down, the RUN button will appear.
6. Click on the Run button to start the run.
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D. Running a User Maintenance Protocol
Some protocols are stored in User Maintenance folder. To run the User Maintenance protocols,
1. Select Maintenance on bottom right of the screen.
2. Select User Maintenance on next screen as shown
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3. From the list of protocols, select the desired protocols and hit Run. See below for an example screen
for protocol selection.
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Item
Frequency
Done By
Cleaning Stage and Racks
After every run
User
Cleaning Magtration Unit
After every run
User
Greasing O-rings
Once every two weeks
User
Replacing O-rings
Every six months
Field Service
Maintenance
Once a year
Field Service
E. About Page
1. Copyright information can be found in the About page. Select About on bottom left corner.
2. Select BACK to go back to the previous screen.
X. Instrument Care and Maintenance
Items for maintenance activity are listed below.
Table IV. Maintenance checklist
• Potential biohazards from samples or extracted/purified products cannot be completely eliminated
while operating the instrument. Use a mask, goggles, and gloves while operating the instrument for
your safety.
• Maintenance work should be done with the instrument powered off.
• Pay close attention when cleaning areas where caution labels are attached.
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NOTES:
• Maintenance work should be done as indicated, otherwise the results of extraction/purification runs may not
be assured.
• Contact us for maintenance work that should be done by Field Service personnel.
• Stage and Racks should be cleaned after every run.
• Nozzles and the Piercing Unit of the Magtration Unit should be cleaned after every run.
A. Cleaning Procedure
Components to be cleaned:
• Stage
• Magtration Unit
• All Racks
• Waste Chute
• Waste Tip Box
Beside the items listed above, all accessories inside the instrument need to be cleaned.
Cleaning method:
Choose liquid depending on the pollution status and wipe the polluted place using paper, such as a
Kimwipe, moistened with a liquid listed below. Use a mask, goggles, and gloves when cleaning the
instrument for your safety.
• Water
• 70% Ethanol
NOTE: Do not use alcohol when cleaning the Touch Panel or the Clear Panel of the Front Door. Use
water for those parts.
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B. Greasing O-rings
Grease O-rings once every two weeks to avoid inadequate picking of tips and/or liquid leakage.
1. Remove old grease.
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2. Wear gloves and put a small amount of silicon grease on your fingertip. Then spread silicon grease by
rubbing it on the O-rings.
3. Put the silicon-greased O-rings (black rubber rings) back on the Nozzles.
4. Use DN70 Tips to make the greased-O-rings fit.
5. Remove all excessive silicon grease from the Nozzle ends and Tip Ejector, using paper, such as a
Kimwipe.
Be sure not to apply too much silicon grease, because it may result in insufficient tip-off operation.
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XI. Troubleshooting
A. Error Reported During Protocol Run
Error code reported is shown in place of “XXXXXX” in the above screen and the corresponding error
message is displayed under the code. Refer to Section XII, “List of Common Error Codes” for a list of
common error codes and countermeasures to be taken.
1. The screen shown above is displayed and the instrument aborts its operation when an error occurs
during a protocol run.
2. Take appropriate countermeasures for the error, referring to the List of Error Codes in Section XII.
3. When contacting us for support, be sure to include
a. A picture of error displayed on the Apollo system touchscreen.
b. A picture of the Apollo system work surface.
c. The name of the protocol running
d. Approximate runtime prior to the crash
e. Any other relevant information
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The protocol run aborted by an error cannot be restarted from the point of suspension.
NOTE: Repeat the same countermeasure when the same error occurs in the next run of the protocol.
Contact us when the countermeasure does not resolve the problem.
B. Suspending or Aborting a Protocol Run
1. When you want to suspend a protocol run operation, select the Suspend button in the display to
suspend instrument operation. It may take some time before the operation is suspended, depending on
the process under execution.
2. Select the START button to restart and continue the protocol run.
3. When you want to abort a protocol run, select the STOP button. A “Protocol Select” screen is
displayed.
The aborted protocol cannot be restarted from its point of suspension.
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Problem
Presumable
Countermeasures to be taken
Power is not
AC Power Cable
Check for connection of AC Power Cable. Be sure to use the
Fuse is burned
Contact us when fuse needs to be replaced.
Displayed
Light intensity is
Adjust light intensity properly by rotating the screw located on
Error code is
Causes vary by
Record the error code displayed and take appropriate
Protocol was
Be sure to confirm appropriate setting of reagents and
Liquid leakage
O-rings are not
Grease O-rings properly or replace O-rings. It is recommended
Disposables are
Check for proper setting of the Microtiter Plates, PCR tubes,
C. Frequently Reported Problems
Table V. Frequently reported problems
cause
supplied to
instrument
characters are not
readable
displayed
started with an
incomplete setting
of tips or other
disposables
is not connected
properly.
out.
low.
error code.
Power Cable supplied with the instrument.
the right of the Display with a flat-blade screwdriver.
countermeasures, referring to the “List of Error Codes” (Section
XII, below). If the countermeasure taken does not eliminate the
problem, contact us for assistance.
disposables, before starting a protocol run. If you want to add
disposables after a protocol is started, press STOP to abort
instrument operation. It is strongly recommended to restart the
protocol run from the beginning after adding disposables.
NOTES:
• If you continue the protocol run from the halted point after
adding disposables, you may not get the desired results.
• When the Front Door is opened after halting operation,
power to the motors is shut for safety, resulting in the
displacement of stopping positions of motors.
from the tip-end or
big difference in
liquid level among
greased enough,
or O-rings are
deteriorated.
lanes
not set properly.
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that replacement of O-rings is made by appropriately trained
field service personnel to avoid damaging the nozzles.
NOTE: Replacing the O-rings by yourself may result in damage
to the instrument.
etc.
NOTE: using disposables other than the ones recommended
may cause instrument malfunction. It is strongly recommended
that you use the disposables designated for each protocol.
Page 30 of 33
Apollo™ System User Manual
Code no.
Presumable cause
Description
Countermeasures to be taken
2001
Axis: error
Axis error
Axis: Hardware end
End Limit Error
2004
Axis: Software end
End Limit Error
2005
Sensor adjustment needed. Contact Field
2006
Failed to initialize
Contact Field Support
2007
Power cycle the instrument first and then Home
2008
Check the deck layout and place tips as
2009
Restart the script from the beginning. A protocol
2010
When Z is moved
Contact Field Support
2019
Door lock parts may need to be replaced
XII. List of Common Error Codes
Table VI. List of error codes
2002 Axis: Z bottom sensor Z button sensor error
2003
limit error
limit error
(Hardware)
(Software)
Interlock error Generation of interlock
Motor
Cannot move XY due
to less height.
ORG-returning error
Cannot find tip
Forcibly closed by
user
Power cycle the instrument first and then Home
all axes by running True_Org_All_axis.scb.
Step-by-step instructions are provided in Section
XIII below
Support at field_support@takarabio.com
all axes by running True_Org_All_axis.scb.
Step-by-step instructions are provided in Section
XIII
appropriate
run cannot be restarted from the point of
suspension
down, Nozzle may hit
Comb
Door lock error
NOTES:
• Most of the errors listed above originate from inadequate setting of disposables/accessories or from an opened
door. Be sure to check those before restarting a protocol run.
• If you encounter error codes other than the ones listed above or the error could not be eliminated by taking the
countermeasure described, contact us with a description of the error codes at field_support@takarabio.com or
technical_support@takarabio.com
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Clear all foreign materials that may be blocking
the door
Page 31 of 33
Appendix
a.
Press
b.
Press
c.
In the window showing the available protocols, press: True_Org_All_axis.scb
A. Recovering a Run After a Crash
Apollo™ System User Manual
If the Apollo system crashes during a run, please follow the instructions below. Call us and a technical
support representative will assist you in trying to recover your samples.
1. Take a picture of error displayed on the Apollo system touchscreen.
2. Take a picture of the Apollo system work surface.
3. Transfer tubes in Block 3 to a 96-well plate, maintaining the orientation of the tubes. Cap the tubes.
Place on ice. (optional)
4. Repeat for Block 4.
5. Send the following information to Field Support at field_support@takarabio.com:
a. Which protocol is running
b. How long has the protocol been running before the error occurred
c. Attach the pictures from 1 and 2
d. Any other relevant information
B. Resetting the Apollo system axes after a crash
If the Apollo system crashes during a run, please follow the instructions below before beginning the next
run. This procedure resets the X, Y, Z and P axes to their default setting.
1. On the Apollo system interface:
2. Press Run to run the script. The script takes <1 minute and resets the X, Y, Z and M axes.
3. When the run is complete, the screen will read End Running and Ok will appear on the bottom left of
the screen.
a. If the Ok button appears, then the Apollo system is reset properly. Press Ok and continue to step 4.
b. If the Ok button does not appear, power off the instrument and repeat Steps 1–3.
4. Power off the Apollo system. Wait at least 60 seconds before powering on again.
5. The Apollo system is now reset and ready to run.
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Page 32 of 33
Apollo™ System User Manual
Contact Us
Customer Service/Ordering
Technical Support
tel: 800.662.2566 (toll-free)
tel: 800.662.2566 (toll-free)
fax: 800.424.1350 (toll-free)
fax: 800.424.1350 (toll-free)
web: takarabio.com
web: takarabio.com
e-mail: ordersUS@takarabio.com
e-mail: techUS@takarabio.com
Notice to Purchaser
Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic
use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to
provide a service to third parties without our prior written approval.
Your use of this product is also subject to compliance with any applicable licensing requirements described on the product’s web page at takarabio.com. It is your
responsibility to review, understand and adhere to any restrictions imposed by such statements
© 2017 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be
registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com
This document has been reviewed and approved by the Quality Department.
.
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Page 33 of 33
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