Sutter Instrument TRIO MP-235 Operation Manual

TRIO™ MP-235

Three-Axis Motorized

Micromanipulator System

With 25mm X & Y Axes, Tilting 50mm D Axis,

Synthetic Z Axis, and USB Interface

Operation Manual

Rev. 2.23 (20190130) (FW v2.2 & 2.3)

One Digital Drive

Novato, CA 94949

Voice: 415-883-0128 Web: www.sutter.com
Fax: [phone] Email: [e-mail]

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Copyright © 2018 Sutter Instrument Company. All Rights Reserved.

TRIO™ is a trademark of Sutter Instrument Company.

TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

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DISCLAIMER

The TRIO MP-235 consists of one electromechanical micromanipulator device and one ROE
(Rotary Optical Encoder) with integrated controller. The purpose of the system is for the
manipulation at the micro level of micropipettes and probes used in conjunction with a
microscope. No other use is recommended.

This instrument is designed for use in a laboratory environment. It is not intended nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.

Unless otherwise indicated in this manual or by Sutter Instrument Technical Support for
reconfiguration, do not open or attempt to repair the instrument.

Do not allow an unauthorized and/or untrained operative to use this device.

Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.

SAFETY WARNINGS AND PRECAUTIONS

Electrical

 Operate the TRIO MP-235 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument is

designed for use in a laboratory environment that has low electrical noise and mechanical
vibration. Surge suppression is recommended at all times

NOTE: There are no user-replaceable fuses in the TRIO MP-235 system.



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switching power adapter. If the external power adapter is damaged due to a mains over or
under voltage, it must be replaced.



electronics, reduces/eliminates electromagnetic interference, and improves the safety of the
system operator. The ROE/controller provides a socket (labeled GROUND) that accepts a
banana plug attached to a suitably gauged insulated wire, the other end of which (alligator
clip) connects to a solid, proper ground.

The TRIO MP-235 system’s power supply consists of an external AC to DC

GROUNDING/EARTHING: Proper grounding protects the ROE/controller

Avoiding Electrical Shock and Fire-related Injury

 Always use the grounded power cord provided to connect the system’s power adapter to a

grounded/earthed mains outlet (3-prong). This is required to protect you from injury in the
event that an electrical hazard occurs.

 Do not disassemble the system. Refer servicing to qualified personnel.



To prevent fire or shock hazard do not expose the unit to rain or moisture.

Electromagnetic Interference

To comply with FDA and CE/EU electromagnetic immunity and interference standards; and
to reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided for interconnecting the electro­mechanical devices and ROE/controller (refer to Technical Specifications for more details).

Operational

Failure to comply with any of the following precautions may damage this device.

TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

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 This instrument is designed for operation in a laboratory environment (Pollution Degree I)

that is free from mechanical vibrations, electrical noise and transients.

DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE



CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do so may result in damage to the electronics.

 Operate this instrument only according to the instructions included in this manual.
 Do not operate if there is any obvious damage to any part of the instrument.



Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and, if undertaken, is done so at the
users’ own risk.

Do not operate if there is any obvious damage to any part of the instrument. Do not



attempt to operate the instrument with the TRIO MP-235/M electromechanical
manipulator shipping tape in place or severe motor damage may result. When transporting
the mechanical manipulator, be sure to reinstall the shipping tape (using masking tape or
equivalent only) to the original locations. Failure to do this may result in damage to the
motors.

Never touch any part of the micromanipulator electromechanical device while it is in



operation and moving. Doing so can result in physical injury (e.g., fingers can be caught
and pinched between the moving parts of the micromanipulator).



If the TRIO MP-235 system is used in a microinjection environment, please observe the
following. As with most micromanipulation devices, sharp micropipettes can fly out of their
holder unexpectedly. Always take precautions to prevent this from happening. Never
loosen the micropipette holder chuck when the tubing is pressurized, and never point
micropipette holders at yourself or others. Always wear safety glasses when using sharp
glass micropipettes with pressure tubing.

Take care to ensure no cables pass close to the TRIO MP-235/M electromechanical

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micromanipulator within the spherical movement limits of all its axes combined.

Other

 Retain the original packaging for future transport of the instrument.
 Sutter Instrument reserves the right to change specifications without prior notice.
 Use of this instrument is for research purposes only.

Handling Micropipettes

Failure to comply with any of the following precautions may result in injury to the users

of this device as well as those working in the general area near the device.

 The micropipettes used with this instrument are very sharp and relatively fragile. Avoid

contact with micropipette tips to prevent accidentally impaling oneself.

 Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps”

container.

TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)

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TABLE OF CONTENTS

DISCLAIMER ..........................................................................................................................................3

SAFETY WARNINGS AND PRECAUTIONS .....................................................................................3

Electrical ..................................................................................................................................................3

Avoiding Electrical Shock and Fire-related Injury.............................................................................. 3

Electromagnetic Interference ............................................................................................................3

Operational ..............................................................................................................................................3

Other ........................................................................................................................................................4

Handling Micropipettes..........................................................................................................................4

1. INTRODUCTION ...............................................................................................................................9

1.1 Structure of the TRIO MP-235 Documentation Package ............................................................9

1.2 Components of the TRIO MP-235 System..................................................................................... 9

1.3 Overview ..........................................................................................................................................10

1.3.1 Features.....................................................................................................................................10

1.3.2 Description................................................................................................................................ 10

2. INSTALLATION...............................................................................................................................11

2.1 Mounting Instructions ...................................................................................................................11

2.1.1 Mounting the TRIO MP-235/M to the Stand or Platform ..................................................11

2.2 Headstage Mounting ......................................................................................................................12

2.3 Other Accessories............................................................................................................................12

2.4 Electrical Connections and Initial Operating Instructions........................................................ 13

2.5 ROE/Controller Rear Panel Controls and Configuration...........................................................14

2.5.1 Power Switch ............................................................................................................................14

2.5.2 Configuration Switches ...........................................................................................................14

2.5.2.1 Switches 1, 2, 3, and 4 .......................................................................................................14

2.5.2.2 Switch 5: Locking Out Y Movement During Homing ...................................................14

2.5.2.3 Switch 6: Sensor Test (Firmware < v2.2) ......................................................................15

2.5.2.4 Switch 6: Calibration Homing on Power On (Firmware v2.2+)..................................15

2.5.2.5 Switches 7 through 10.......................................................................................................15

2.5.2.6 Switch 7: Speed of Manual Movement (Firmware v2.2+)............................................15

2.5.2.7 Switch 8: Reserved (Firmware <= v2.2)........................................................................16

2.5.2.8 Switch 8: ROE Axis Movement Knobs Active – All vs. D Only (Firmware v2.3+)....16

2.5.2.9 Switch 9: Y-Axis Travel Length (Firmware v2.2+).......................................................16

2.5.2.10 Switch 10: X Axis Travel Length (Firmware v2.2+)................................................... 16

3. OPERATIONS...................................................................................................................................17

3.1 Main Controls and Indicators on the ROE/Controller ...............................................................17

3.2 Display..............................................................................................................................................18

3.2.1 Initial Startup...........................................................................................................................18

3.3 Control Operations .........................................................................................................................18

3.3.1 Maximum Positive Position Values: ......................................................................................18

3.3.2 Setting Position for HOME or WORK...................................................................................18

3.3.3 Setting the Angle of the Pipette/Headstage Holder .............................................................19

3.3.4 Operating the Virtual Z Axis...................................................................................................19

3.3.5 Moving to the Home Position (HOME) ................................................................................. 19

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3.3.6 Moving to the Work Position (WORK) ..................................................................................20

3.3.7 Setting Absolute/Relative Coordinates Mode (RELATIVE)................................................20

3.3.8 Mode Indications ......................................................................................................................21

3.3.9 Speed Control and ROE Knob Movements (SPEED)..........................................................21

3.3.10 Movement Knobs Disabling and Lock Mode ([SPEED]/LOCK) ......................................21

3.3.11 Pausing Home Movements (HOME (while moving to Home)) ........................................21

3.3.12 Pausing Work Movements (WORK (while moving to Work)) ..........................................21

3.3.13 Pulse Mode and D-Axis Movement (PULSE) .....................................................................21

4. EXTERNAL CONTROL...................................................................................................................22

4.1 General.............................................................................................................................................22

4.2 Virtual COM Port (VCP) Serial Port Settings.............................................................................22

4.3 Protocol and Handshaking ............................................................................................................22

4.4 Command Sequence Formatting ..................................................................................................22

4.5 Axis Position Command Parameters............................................................................................23

4.6 Microsteps and Microns (Micrometers)........................................................................................23

4.7 Commands ....................................................................................................................................... 24

4.7.1 Get Current Position (‘c’ or ‘C’) Command ..........................................................................24

4.7.2 Move to Controller-Defined HOME Position (‘h’) Command.............................................24

4.7.3 Move to Controller-Defined WORK Position (‘w’) Command ............................................24

4.7.4 Move to Specified “Home” Position (‘H’) Command ...........................................................25

4.7.5 Move to Specified “Work” Position (‘W’) Command............................................................25

4.7.6 Move to Specified X-Axis Position (‘x’ or ‘X’) Command.....................................................25

4.7.7 Move to Specified Y-Axis Position (‘y’ or ‘Y’) Command..................................................... 26

4.7.8 Move to Specified D-Axis Position (‘d’ or ‘D’) Command ....................................................26

4.7.9 External Command Notes....................................................................................................... 26

5. MAINTENANCE...............................................................................................................................28

6. RECONFIGURATION .....................................................................................................................28

6.1 Changing the Rotary Knob Functions on the ROE/Controller .................................................28

APPENDIX A. LIMITED WARRANTY..............................................................................................29

APPENDIX B. ACCESSORIES............................................................................................................29

APPENDIX C. TECHNICAL SPECIFICATIONS .............................................................................30

APPENDIX D. QUICK REFERENCE................................................................................................31

D.1. Manual Operation.........................................................................................................................31

D.2. Configuration.................................................................................................................................31

D.3. External Control ...........................................................................................................................32

INDEX ....................................................................................................................................................37

TABLE OF FIGURES

Figure 1-1. The TRIO MP-235 system..................................................................................................... 9

Figure 2-1. Side view of TRIO MP-235/M showing mounting adapter plate and lock screws......... 11

Figure 2-2. Mounting the TRIO MP-235/M on the Adapter Plate...................................................... 12

TRIO MP-235 THREE-AXIS MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.23 (20190130) (FW V2.2 & 2.3)