Sutter Instrument MP-285, ROE Basic Operations
MP-285
Micromanipulator System
ROE
Basic Operations
Rev. 3.08 ( 20140809)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax: 415-883-0572 Email: info@sutter.com
ii
Copyright © 2014 Sutter Instrument Company. All Rights Reserved.
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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Mains Voltage
DIS CLAI M ER
The
MP-285
-system consists of one controller (MP-285), a user control device (ROE or
Joystick), and one electromechanical micromanipulator device (MP-285/M). The purpose of
the system is for the manipulation at the micro level of micropipettes and probes used in
conjunction with a microscope. No other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended, nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Do not open or attempt to repair the instrument. High voltages are present and inadvertent
movement of the micromanipulator electromechanical could cause injury.
Do not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAF E TY W ARN ING S AND PRE CAU T ION S
Electrical
Operate the MP-285 using 110-- 240 V AC., 50-60 Hz line voltage. This instrument is
designed for use in a laboratory environment that has low electrical noise and mechanical
vibration. Surge suppression is recommended at all times.
Fuse Replacement: Replace only with the same type and rating:
Mains fuse type and rating.
Fuse
Setting
“110”
(100 – 120
VAC)
“220”
(200 – 240
VAC)
Rating Manufacturer Examples
2A, 250V
(Time Delay)
T1.0A, 250V Bussmann: GDC-1A or S506-1A (RoHS)
(Type: Time Delay, 5mm x 20mm, glass tube)
Bussmann: GMC-2A, GMC-2-R (RoHS), GDC-2A, or
S506-2A (RoHS)
Littelfuse: 239 002 or 239.002.P (RoHS)
Littelfuse: 218 001 or 218 001.P (RoHS)
A spare fuse is located in the power input module. Please refer to the fuse-replacement
appendix for more details on fuse ratings and for instructions on how to change the fuse.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power supply cord set provided to connect the system to a
grounded outlet (3-prong). This is required to protect you from injury in the event that
an electrical hazard occurs.
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
iv
Do not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE electromagnetic immunity and interference standards; and to
reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the interconnection
of the ROE, MP-285/M electromechanical device, and host computer via serial RS-232
interface, (see the Technical Specifications appendix for more details).
Operational
Failure to comply with any of the following precautions may damage this device.
This instrument is designed for operation in a laboratory environment (Pollution Degree
I) that is free from mechanical vibrations, electrical noise and transients.
This unit is not designed for operation at altitudes above 2000 meters nor was it tested
for safety above 2000 meters.
DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do this may result in damage to the electronics.
Operate this instrument only according to the instructions included in this manual.
Do not operate if there is any obvious damage to any part of the instrument.
Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and if undertaken is done so at the
users’ own risk.
Do not attempt to operate the instrument with the manipulator shipping screws in
place - severe motor damage may result.
Do not operate if there is any obvious damage to any part of the instrument. Do not
attempt to operate the instrument with the manipulator shipping screws in place - severe
motor damage may result. When transporting the mechanical manipulator, be sure to
install the shipping screws supplied in their correct locations. Failure to do this may
result in damage to the motors.
Never touch any part of the micromanipulator electromechanical device while it is in
operation and moving. Doing so can result in physical injury (e.g., fingers can be caught
and pinched between the moving parts of the micromanipulator).
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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As with all microinjection devices, sharp micropipettes can fly out of their holder
unexpectedly. Always take precautions to prevent this from happening. Never loosen the
micropipette holder chuck when the tubing is pressurized, and never point micropipette
holders at yourself or others. Always wear safety glasses when using sharp glass
micropipettes with pressure microinjectors.
Do not handle the manipulator mechanical while the power is on, and take care to
ensure no cables pass close to the mechanical manipulator.
Other
Use this instrument only for microinjection purposes in conjunction with the procedures
and guidelines in this manual.
Retain the original packaging for future transport of the instrument.
Some applications, such as piezo-impact microinjection call for the use of mercury in the
micropipette tip. The use of any hazardous materials with any Sutter Instrument’s
instrument is not recommended and if undertaken is done so at the users’ own risk.
When transporting the mechanical manipulator, be sure to install the shipping screws
supplied in their correct locations. Failure to do this may result in damage to the motors.
This instrument contains no user-serviceable components — do not open the instrument
casing. This instrument should be serviced and repaired only by Sutter Instrument or an
authorized Sutter Instrument servicing agent.
Sutter Instrument reserves the right to change specifications without prior notice.
This device is intended only for research purposes.
Handling Micropipettes
Failure to comply with any of the following precautions may result in injury to the users
of this device as well as those working in the general area near the device.
The micropipettes used with this instrument are very sharp and relatively fragile.
Contact with the pulled micropipette tips, therefore, should be avoided to prevent
accidentally impaling yourself.
Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps”
container.
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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TABLE OF CONTENTS
DISCLAIMER ......................................................................................................................................iii
SAFETY WARNINGS AND PRECAUTIONS...................................................................................iii
Electrical .................................................................................................................................................iii
Avoiding Electrical Shock and Fire-related Injury.............................................................................iii
Electromagnetic Interference...............................................................................................................iv
Operational.............................................................................................................................................iv
Other.........................................................................................................................................................v
Handling Micropipettes..........................................................................................................................v
1. GENERAL INFORMATION...........................................................................................................1
1.1 Structure of the MP-285 Documentation Package ...............................................................................1
1.2 Technical Support.............................................................................................................................1
2. SETUP...............................................................................................................................................3
2.1 Unpacking..........................................................................................................................................3
2.2 Initial Setup and Gross Movement.................................................................................................4
2.3 Installation of the MP-285 Micromanipulator on your Microscope............................................6
2.3.1 General Considerations.............................................................................................................6
2.3.2 Specifics.......................................................................................................................................6
2.4 Mounting Headstages.......................................................................................................................7
2.5 Adjusting the Rotating Dovetail Base.............................................................................................8
2.6 Minimizing Electrical Noise ............................................................................................................9
3. MICROSCOPIC MANIPULATION ..............................................................................................11
3.1 Some Basic Information.................................................................................................................11
3.2 SETUP Selection ............................................................................................................................11
3.3 Changing Minimal Resolution.......................................................................................................12
4. PULSE MOVEMENT.....................................................................................................................15
5. HOME FUNCTION........................................................................................................................17
5.1 How to Define a New Home ..........................................................................................................17
5.2 Moving to the New Home:.............................................................................................................17
5.3 Practical Use of the Home Function.............................................................................................17
6. GENERALIZED ROBOTIC MOVEMENTS.................................................................................19
6.1 Setting Absolute Zero.....................................................................................................................19
6.2 Redefining Home ............................................................................................................................19
6.3 Clearing Program Location #1 for Recording a New Robotic Series .......................................19
6.4 Teaching the MP-285 Controller a New Robotic Series.............................................................20
6.5 Executing the Newly Entered Program!......................................................................................21
6.6 Loading a Saved Program..............................................................................................................22
7. DIAGONAL OR 4TH AXIS MOVEMENTS .................................................................................23
8. ROE RESPONSE CHARACTERISTICS ......................................................................................25
8.1 Controlling ROE Knob to Manipulator Stepper Motor Axis Assignment................................25
8.2 Controlling Movement Amount per ROE Turn..........................................................................25
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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APPENDIX A. LIMITED WARRANTY............................................................................................27
INDEX.................................................................................................................................................29
TABLE OF FIGURES
Figure 2-1. MP-285 Controller (front panel)...........................................................................................3
Figure 2-2. Manipulator. Figure 2-3. Rotary Optical Encoder (ROE)...............................................3
Figure 2-4. MP-285 controller rear connections. ....................................................................................4
Figure 2-5. Manipulator base dimensions................................................................................................6
Figure 2-6. Headstage mounting plate.....................................................................................................7
Figure 2-7. Mounting the headstage on the manipulator's Z-axis.........................................................8
Figure 2-8. Adjusting the headstage angle...............................................................................................8
TABLE OF TABLES
Table 3-1. Microsteps per ROE turn settings........................................................................................12
Table 3-2. Microns per ROE turn settings.............................................................................................13
Table 4-1. Microsteps and microns per Pulse settings. ........................................................................16
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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1. GEN E RAL INFO RMA TION
1.1 Structure of the MP-285 Documentation Package
The MP-285 is a highly versatile stepper motor driven micromanipulator. Because of this
versatility, the documentation necessary to cover various functions has rapidly grown to a
size best described as unwieldy and thus has generally not been useful to most operators.
In order to produce a more useful set of instructions, we have split the manual into two
parts. The first part, “Basic Operations”, has been kept short and covers major functions
with the aim of quickly guiding the first time user through the setup and use of the MP-285
for micromanipulation with emphasis on using the ROE (Rotary Optical Encoder) for user
control. These steps are, in many ways, identical to the steps performed any time one sets up
an MP-285 for use at a microscope. Many users will find that no other manual is necessary.
The second part, “Reference Manual”, provides in-depth coverage of the MP-285. It
documents the precise keystrokes (menu navigation) necessary to access each MP-285
function. The Reference Manual also describes, in detail, how to alter MP-285 performance
via add-on accessories, perform microcontroller robotic programming, and how to use the
serial computer interface.
1.2 Technical Support
Unlimited technical support is provided by Sutter Instrument Company at no charge to our
customers. Our technical support staff is available between the hours of 8:00 AM and 5:00
PM (Pacific Time) at
(415) 883-0128
. You may also E-mail your queries to
info@sutter.com
.
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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HOM
NOR
COAR
CON
DIA
FIN
PULS
2. SETU P
2.1 Unpacking
Carefully remove all components from the shipping container. One of each of the following
should be included:
MP-285 controller
MP-285 micromanipulator and D25 cable for connection to controller
Rotary Optical Encoder (ROE) and D15 cable for connection to controller
Controller power cord
Serial port cable (DB9 connectors)
Metal dovetail headstage mounting plate with plastic isolation plate
Metal dovetail rod clamp
2.5mm hex wrench for removing the shipping screws
7/64 hex wrench for adjusting the rotating dovetail base
MP-285 documentation
RESET
POWER
MP-285
SUTTER INSTRUMENT CO.
Figure 2-1. MP-285 Controller (front panel).
MADE IN U.S.A.
Figure 2-2. Manipulator. Figure 2-3. Rotary Optical Encoder (ROE).
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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1 2 3 4 5 6 7 8 9 10 11 12 13
14 15 16 17 18 19 20 21 22 23 24 25
1 2 3 4 5 6 7 8
9 10 11 12 13 14 15
110
220
FUSE
L
SUTTER
INSTRUMENT
51 Digital Drive
NOVATO, CA 94949 USA
(415) 883 - 0128
DISCONNECT POWER
BEFORE OPENING
UNIT!
MODEL
Hz
FUSE
VOLTS
DANGER
SERIAL PORT
MANIPULATOR
COMMAND INP
UT
You may have also received one or more accessories for mounting your MP-285 and/or
modifying the headstage mount to the manipulator (i.e., rotating base, microscope stage
mount, gantry stand, dovetail extension). Setup of these accessories is covered either in
documentation accompanying the accessory or in the
MP-285 Reference Manual
.
Remove all six shipping screws from the MP-285. Save the screws, warning tags, and hex
wrench in the event you need to transport your manipulator in the future. Once these screws
have been removed, handle the micromanipulator with care. The drive cables can be
damaged
2.2 Initial Setup and Gross Movement
Initially, you may want to simply connect your manipulator, controller, and ROE together and
try some gross movements in order to get a feel for the controls and how to make simple
movements. It is perfectly acceptable to set the manipulator in the middle of a bench top,
make all electrical connections and then observe the unit’s movement by eye. Even if you
wish to directly install your manipulator in your rig, it is useful to follow the initial setup
procedure in order to learn how to move the unit and allow easy access to the mounting
screws if subjected to unnecessary strain.
1. Connect the power cord to the MP-285 controller. Make certain that the voltage selector
next to the power cord socket on the back of the MP-285 controller has been set to the
appropriate voltage (110 or 220).
ABEL
VOLTAGE
SELECTOR
HIGH VOLTAGE
LINE POWER
FUSE
Figure 2-4. MP-285 controller rear connections.
INPUT DEVICE
CABLE (15 pin)
SERIAL PORT
CABLE (9 pin)
MANIPULATOR
CABLE (25 pin)
2. With the power OFF (front panel switch in the down position), use the D15 cable and
connect the ROE to the connection labeled “COMMAND INPUT” on the back of the MP285 controller.
CAUTION: Never connect or disconnect the ROE while the power is on!
3. With the power OFF, use the D25 cable and connect the MP-285 micromanipulator to the
DB 25 connector labeled “MANIPULATOR” on the back of the controller.
CAUTION: Never connect or disconnect the micromanipulator while the power is on!
MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 3.08 (20140809)
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