SUTTER INSTRUMENT P-30 User Manual

P----30

Micropipette Puller

Micropipette PullerMicropipette Puller

Operation Manual

Rev. 3.10 ( 20100120)

3030

Sutter Instrument Company

One Digital Drive

Novato, CA 94949

Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com



No part of this manual may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from Sutter Instrument Company.

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

III

Mains Voltage

Mains Voltage Mains Voltage

Mains Voltage

DIS C LAIM ER

DIS C LAIM ERDIS C LAIM ER

The pipette puller Model P-30 is designed for the specific use of creating micropipettes and no other use is recommended.

This instrument creates items that should only be used in a laboratory environment for use on animal tissues. It is not intended for use, nor should be used, in human experimentation, or applied to humans in any way. This is not a medical device.

Do not open or attempt to repair the instrument without expressed and explicit instructions from Sutter Instrument Company. Extreme heat and high voltages are present and could cause injury.

Do not allow unauthorized and or untrained operatives to use this device.

Any misuse will be the sole responsibility of the user/owner and Sutter Instruments assumes no implied or inferred liability for direct or consequential damages from this instrument if it is operated or used in any way other than for which it is designed.

SAF E TY W ARNI N G

SAF E TY W ARNI N GSAF E TY W ARNI N G





Replace fuse only with the same type and rating as indicated in the following table.

Replace fuse only with the same type and rating as indicated in the following table. Replace fuse only with the same type and rating as indicated in the following table.

Fuse

FuseFuse

(Type: T

Setting

SettingSetting

Rating

Rating Manufacturer Examples

RatingRating

“110”

(100 – 120

VAC)

“220”

(200 – 240

VAC)

A spare fuse is provided, which is located in the power input module. Please refer to the

A spare fuse is provided, which is located in the power input module. Please refer to the A spare fuse is provided, which is located in the power input module. Please refer to the Fuse Replacement appendix located in the end portion of this manual.

Fuse Replacement appendix located in the end portion of this manual.

Fuse Replacement appendix located in the end portion of this manual.Fuse Replacement appendix located in the end portion of this manual.



To prevent fire or sho

To prevent fire or shock hazard do not expose the unit to rain or moisture.

To prevent fire or shoTo prevent fire or sho



To avoid electrical shock:

To avoid electrical shock:To avoid electrical shock:

•

Do not disassemble the unit. Refer servicing to qualified personnel.

Do not disassemble the unit. Refer servicing to qualified personnel.Do not disassemble the unit. Refer servicing to qualified personnel.

•

Always use the grounded power supply cord set provided to connect the unit to a

Always use the grounded power supply cord set provided to connect the unit to a Always use the grounded power supply cord set provided to connect the unit to a grounded outlet (3

grounded outlet (3----prong)

grounded outlet (3grounded outlet (3 that an electrical hazard develops.

that an electrical hazard develops.

that an electrical hazard develops.that an electrical hazard develops.

2A, 250V

(Time Delay)

T1.25A,

250V

ck hazard do not expose the unit to rain or moisture.

ck hazard do not expose the unit to rain or moisture.ck hazard do not expose the unit to rain or moisture.

prong). This is required to protect you from injury in the event

prong)prong)

(Type: Time Delay, 5mm x 20mm, glass tube)

(Type: T(Type: T

. This is required to protect you from injury in the event

. This is required to protect you from injury in the event . This is required to protect you from injury in the event

ime Delay, 5mm x 20mm, glass tube)

ime Delay, 5mm x 20mm, glass tube)ime Delay, 5mm x 20mm, glass tube)

Manufacturer Examples

Manufacturer ExamplesManufacturer Examples

Bussmann: GMC-2A, GMC-2-R (RoHS), GDC-2A, or S506-2A (RoHS)

Littelfuse: 239 002 or 239.002.P (RoHS)

Bussmann: GDC-1.25A or S506-1.25R (RoHS) Littelfuse: 218 1.25 or 218 1.25P (RoHS)



To avoid burns do not touch the heating filament, the brass clamps holding the filament

To avoid burns do not touch the heating filament, the brass clamps holding the filament To avoid burns do not touch the heating filament, the brass clamps holding the filament or the heated ends of glass pipettes that have been pulled.

or the heated ends of glass pipettes that have been pulled.

or the heated ends of glass pipettes that have been pulled.or the heated ends of glass pipettes that have been pulled.

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

PRE C AUTI O N

PRE C AUTI O NSSSS

PRE C AUTI O NPRECA U TIO N

On Operation

On OperationOn Operation

Failure to comply with any of the following precautions may damage this device.

Failure to comply with any of the following precautions may damage this device. Failure to comply with any of the following precautions may damage this device.

Failure to comply with any of the following precautions may damage this device.

Failure to comply with any of the following precautions may damage this device. Failure to comply with any of the following precautions may damage this device.



Operate the P

Operate the P----30 using 110V a.c. and 60Hz, or 220V

Operate the POperate the P



The P

The P----30 is designed for operation in a laboratory environment (pollution degree I).

The PThe P



The P

The P----30 is designed for connection to a standard laboratory power outlet (overvoltage category

The PThe P II).

II).

II).II).



This unit was not designed for operation

This unit was not designed for operation at altitudes above 2000 meters nor was it tested for

This unit was not designed for operationThis unit was not designed for operation safety above 2000 meters.

safety above 2000 meters.

safety above 2000 meters.safety above 2000 meters.



Only use Sutter Instrument Company replacement heating filaments.

Only use Sutter Instrument Company replacement heating filaments.Only use Sutter Instrument Company replacement heating filaments.

On Handling Micropipettes

On Handling MicropipettesOn Handling Micropipettes

Failure to comply with any of the following precautions

Failure to comply with any of the following precautions may result in injury to the users of

Failure to comply with any of the following precautionsFailure to comply with any of the following precautions this device as well as those working in the general area near the device.

this device as well as those working in the general area near the device.

this device as well as those working in the general area near the device.this device as well as those working in the general area near the device.

30 is designed for operation in a laboratory environment (pollution degree I).

30 is designed for operation in a laboratory environment (pollution degree I).30 is designed for operation in a laboratory environment (pollution degree I).

30 is designed for connection to a standard laboratory power outlet (overvoltage category

30 is designed for connection to a standard laboratory power outlet (overvoltage category 30 is designed for connection to a standard laboratory power outlet (overvoltage category

30 using 110V a.c. and 60Hz, or 220V a.c. and 50Hz line voltage.

30 using 110V a.c. and 60Hz, or 220V30 using 110V a.c. and 60Hz, or 220V

at altitudes above 2000 meters nor was it tested for

at altitudes above 2000 meters nor was it tested for at altitudes above 2000 meters nor was it tested for

a.c. and 50Hz line voltage.

a.c. and 50Hz line voltage. a.c. and 50Hz line voltage.

may result in injury to the users of

may result in injury to the users of may result in injury to the users of



The micropipettes created using this instrument are very sharp and relatively fragile. Contact

The micropipettes created using this instrument are very sharp and relatively fragile. Contact The micropipettes created using this instrument are very sharp and relatively fragile. Contact with the pulled micropipette tips, therefore, sh

with the pulled micropipette tips, therefore, should be avoided to prevent accidentally impaling

with the pulled micropipette tips, therefore, shwith the pulled micropipette tips, therefore, sh yourself.

yourself.

yourself. yourself.



Always dispose of micropipettes by placing them into a well

Always dispose of micropipettes by placing them into a well----marked, spill

Always dispose of micropipettes by placing them into a wellAlways dispose of micropipettes by placing them into a well container.

container.

container.container.



Use only with glassware recommended by Sutter Instrument Company in the following section of

Use only with glassware recommended by Sutter Instrument Company in the following section of Use only with glassware recommended by Sutter Instrument Company in the following section of th

this manual.

is manual.

thth

is manual.is manual.

ould be avoided to prevent accidentally impaling

ould be avoided to prevent accidentally impaling ould be avoided to prevent accidentally impaling

marked, spill----proof “sharps”

marked, spillmarked, spill

proof “sharps”

proof “sharps” proof “sharps”

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

TABLE OF CONTENTS

TABLE OF CONTENTSTABLE OF CONTENTS

DISCLAIMER

DISCLAIMER ................................

DISCLAIMERDISCLAIMER

SAFETY WARNING

SAFETY WARNING ................................

SAFETY WARNINGSAFETY WARNING

PRECAUTION

PRECAUTIONSSSS ................................

PRECAUTIONPRECAUTION

On Operation..........................................................................................................................................iv

On Handling Micropipettes ..................................................................................................................iv

1. Introduction

Introduction................................

1.1.

Introduction Introduction

1.1 Technical Support.............................................................................................................................1

1.2 Glass Capillary & Heating Filament Specifications......................................................................1

2. Product Description

Product Description ................................

2.2.

Product Description Product Description

2.1 Packing List.......................................................................................................................................3

2.2 Front Panel........................................................................................................................................3

2.2.1 Lower Panel Display and Controls...........................................................................................3

2.2.2 Upper Panel Display and Controls...........................................................................................3

2.3 Mechanical Description (Puller Anatomy).....................................................................................4

2.3.1 Slide Assembly............................................................................................................................4

2.3.2 Pull Solenoid...............................................................................................................................5

2.3.3 Patch Block (Figure 4-1)............................................................................................................5

................................................................

...........................................................

................................................................

..........................................................

................................................................

......................................

................................................................

...........................iii

......................................................

..................................

................................................................

.......................................

................................................................

..........................3333

....................................................

......iii

iii

............

iiiiii

iii

iiiiii

..iv

....

iviv

.......1111

..............

3. Installation

Installation ................................

3.3.

Installation Installation

3.1 Line Power (Mains) ..........................................................................................................................7

4. Operating Instructions

Operating Instructions ................................

4.4.

Operating Instructions Operating Instructions

4.1 First Time Use ..................................................................................................................................9

4.2 General Operation ............................................................................................................................9

4.3 Pulling a Micropipette......................................................................................................................9

4.4 Multicycle Pulling: Pulling a Patch-Type Micropipette .............................................................10

4.4.1 Heat Settings............................................................................................................................10

4.4.2 Pull Setting ...............................................................................................................................10

4.4.3 Trip Point Micrometer ............................................................................................................10

4.4.4 Patch Block (spacer) ................................................................................................................11

4.5 Patch Pipette Pull Sequence..........................................................................................................11

4.5.1 First cycle..................................................................................................................................11

4.5.2 Second cycle ..............................................................................................................................11

4.6 Controls Adjustment ......................................................................................................................12

5. Maintenance

Maintenance................................

5.5.

Maintenance Maintenance

5.1 Filament Replacement ...................................................................................................................15

5.2 Lubrication ......................................................................................................................................15

5.3 Rubber Pads on Clamps.................................................................................................................16

5.4 Troubleshooting..............................................................................................................................16

APPENDIX A.

APPENDIX A. Limited Warranty

APPENDIX A.APPENDIX A.

................................................................

Limited Warranty................................

Limited Warranty Limited Warranty

................................................................

.....................................................

................................................................

........................................

................................................................

.....................9999

..........................................

....................................

................................................................

......................................

................................................................

........7777

................

......19

............

....15

........

1515

1919

APPENDIX B.

APPENDIX B. Accessories

APPENDIX B.APPENDIX B.

Nichrome filament block assembly (P-30-NFL/M) ...........................................................................21

Accessories ................................

Accessories Accessories

................................................................

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

................................................................

.................................................

................................................................

.................21

..................................

2121

Platinum/iridium filament block assembly (P-30-PFL/M)...............................................................21

Pipette storage box 4 3/4 x 3 5/8 x 3/4 inches (BX10)........................................................................21

Pipette storage box - long 7 x 3 5/8 x 3/4 inches (BX20) ...................................................................21

BV-10 Microelectrode Beveler (BV-10)...............................................................................................21

Glass Capillary Tubing.........................................................................................................................21

Puller Filaments....................................................................................................................................21

APPENDIX C.

APPENDIX C. Fuse Replacement

APPENDIX C.APPENDIX C.

APPENDIX D.

APPENDIX D. Technical Specifications

APPENDIX D.APPENDIX D.

INDEX

INDEX................................

INDEXINDEX

TABLE OF FIGURES

TABLE OF FIGURESTABLE OF FIGURES

Figure 2-1. P-30 Mechanical Assembly....................................................................................................6

Figure 3-1. P-30 Cabinet (rear view)........................................................................................................7

Figure 4-1. Patch Block Placement. .......................................................................................................12

Figure 5-1. Top view of platinum filament glass...................................................................................15

Figure C-1. Fuse holder............................................................................................................................23

................................................................

Fuse Replacement ................................

Fuse Replacement Fuse Replacement

Technical Specifications................................

Technical Specifications Technical Specifications

................................................................

.....................................

................................................................

............................................................

................................................................

.................................................

................................................................

............................25

........................................................

.................27

..................................

.....23

..........

2323

2525

2727

TABLE OF TABLES

TABLE OF TABLESTABLE OF TABLES

Table C-1. Mains fuse type and ratings..................................................................................................23

Table D-1. Mains fuse type and ratings. ................................................................................................25

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

1. IN TROD U CTI O N

INT R ODUC TION

1.1.

INT R ODUC TIONIN TROD UCTI O N

The P-30 Micropipette Puller is a derivative of a vertical puller design initially developed at the National Institutes of Health in the late 1950’s. The P-30 is designed to be a basic, economical and simple to operate puller for those investigations where tip diameters down to

0.3 micron and moderate taper lengths are satisfactory. Despite its low cost, the P-30 has been designed with a number of quality and convenience features. The power supplies for the filament and pull solenoid are constant current and are thermally protected. Dual heat settings are provided for multi-step pipette fabrications such as are used in patch clamp investigations. The front cover completely encloses the pulling mechanism to prevent erratic results caused by drafts; and the front panel is sloped to prevent broken capillaries from entering the solenoid area. In addition, CMOS logic is used throughout for its high noise immunity and low power consumption.

This manual, though brief, describes all that you need to know to operate the P-30. The following pages provide descriptions of the mechanical assembly, controls and displays along with basic information on using the puller. The P-30 provides a consistent, reproducible environment for the manufacture of micropipettes. Its successful use will depend on providing the minimal maintenance detailed on the following pages, information on desirable pulling conditions obtained from colleagues and the literature; and finally, from your own experience and experimentation.

Throughout this manual reference will be made to the size of the glass tubing used to pull micropipettes. The convention used here for describing the outside diameter (O.D.) and inside diameter (I.D.) is as follows: O.D. x I.D. These dimensions will always be given in millimeters (mm).

1.1

1.1 Technical Support

Technical Support

1.11.1

Technical SupportTechnical Support

Unlimited technical support is provided by Sutter Instrument Company at no charge to our customers. Our technical support staff is available between the hours of 8:00 AM and 5:00 PM (Pacific Standard Time) at (415) 883

info@sutter.com

info@sutter.com.

info@sutter.cominfo@sutter.com

1.2

1.2 Glass Capillary & Heating Filament Specifications

Glass Capillary & Heating Filament Specifications

1.21.2

Glass Capillary & Heating Filament SpecificationsGlass Capillary & Heating Filament Specifications

The P-30 micropipette puller is designed for use with borosilicate, aluminosilicate or other lower melting-point glass tubing or rod ranging from 1.0mm to 2.0mm in diameter. This instrument does not pull fused silica or quartz glassware. The full selection of glassware available from Sutter Instrument Co. is listed in the catalogue included with this instrument or can be found on the Sutter Instrument Company’s World Wide Web site www.sutter.com

This instrument is designed to accommodate either a Nichrome wire filament or the Sutter Trough-type platinum filaments that are shown in the Sutter Catalogue. The selection of replacement filaments for the P-30 can also be viewed on Sutter Instrument Company’s World Wide Web site at wwwwww.sutter.com REPLACEMENT HEATING FILAMENTS IN THIS INSTRUMENT! Instructions for replacing the Heating Filament can be found in the Maintenance section of this manual.

(415) 883----0128

(415) 883(415) 883

ww.sutter.com. ONLY USE SUTTER-SUPPLIED

ww.sutter.comww.sutter.com

0128. You may also E-mail your queries to

01280128

www.sutter.com.

www.sutter.comwww.sutter.com

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

(This page intentionally left blank.)

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

2. PR ODU C T DE S CRI P TION

PROD UCT D ESC RIPTI ON

2.2.

PROD UCT D ESC RIPTI ONPRODU CT D ESCR I PTIO N

2.1

2.1 Packing List

Packing List

2.12.1

Packing ListPacking List

Make certain that you have received all of the following items in the P-30 shipping box:



PPPP----30 micropipette puller

30 micropipette puller

30 micropipette puller30 micropipette puller



Power cord

Power cordPower cord



Box of sample glass

Box of sample glassBox of sample glass



3 Spare heating filaments

3 Spare heating filaments3 Spare heating filaments



Patch Block attachment

Patch Block attachmentPatch Block attachment



Warranty

Warranty registration

Warranty Warranty



Catalog

CatalogCatalog

The Model P-30 is shipped to you in a prefabricated foam mold. Please take note of this method of packaging. Should it ever be necessary to ship the puller to another location, the same method of packaging should be employed. Additional packing material may be purchased from Sutter Instrument Company.

registration

registrationregistration

IMPORTANT: Improper packaging is a form of abuse and, as such, can be responsible for

IMPORTANT: Improper packaging is a form of abuse and, as such, can be responsible for IMPORTANT: Improper packaging is a form of abuse and, as such, can be responsible for voiding the warranty where shipping damage is sustained because of such packing.

voiding the warranty where shipping damage is sustained because of such packing.

voiding the warranty where shipping damage is sustained because of such packing.voiding the warranty where shipping damage is sustained because of such packing.

2.2

2.2 Front Panel

Front Panel

2.22.2

Front PanelFront Panel

The front panel controls are used to apply power to the instrument, set heat and pull strength values and to start and stop pulling sequences. Controls on the lower portion of the front panel will be described first.

2.2.1

2.2.1 Lower Panel Display and

Lower Panel Display and Controls

2.2.12.2.1

Lower Panel Display and Lower Panel Display and

On/Off Switch

On/Off Switch On applies power to the instrument. Red lamp is illuminated when

On/Off SwitchOn/Off Switch

power is on.

Reset Switch

Reset SwitchReset Switch

Pull Switch

Pull SwitchPull Switch

2.2.2

2.2.2 Upper Panel Display and Controls.

Upper Panel Display and Controls.

2.2.22.2.2

Upper Panel Display and Controls.Upper Panel Display and Controls.

Used to reset circuitry to an initialized condition. It is typical use is to turn off the heat during multi-step fabrications (patch type pipettes).

Initiates the pulling process.

Controls

ControlsControls

Heat 1/Heat 2 switch

Heat 1/Heat 2 switch This switch determines which of the two heat values is active.

Heat 1/Heat 2 switchHeat 1/Heat 2 switch

HEAT 1 control

HEAT 1 control HEAT 1 is a three-digit pushbutton control used to set the level of

HEAT 1 controlHEAT 1 control

electrical current supplied to the filament. The HEAT required to

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

melt a piece of glass is a function of the filament installed and the particular glass size and composition. Maximum current available is

22.5 amperes

HEAT 2 control

HEAT 2 control Same as Heat 1.

HEAT 2 controlHEAT 2 control

PULL Strength

PULL Strength PULL Strength control

control

controlcontrol

A three-digit pushbutton control used to set the level of electrical current supplied to the pull solenoid.

This parameter controls the force of the hard pull. In general, the higher the PULL, the smaller the pipette’s tip diameter and the longer the taper.

Trip Point

Trip Point Trip Point Micrometer (

Micrometer (Figure

Micrometer (Micrometer ( 2-1H)

H)H)

A micrometer with 0.001-inch divisions that is used to adjust the distance to which the glass draws out before a hard pull is initiated.

2.3

2.3 Mechanical Description (Puller Anatomy)

Mechanical Description (Puller Anatomy)

2.32.3

Mechanical Description (Puller Anatomy)Mechanical Description (Puller Anatomy)

This section presents a basic mechanical description of the P-30, with particular emphasis on terminology. Knowing the names of the various parts greatly facilitates communication between the investigators and the manufacturer when discussing adjustments or service problems.

2.3.1

2.3.1 Slide Assembly

Slide Assembly

2.3.12.3.1

Slide AssemblySlide Assembly

The Slide Assembly

Slide Assembly consists of all the mechanical parts that make up the glass pulling

Slide AssemblySlide Assembly

mechanism. Its elements are described below.

Upper (Stationary) Glass Clamp As

Upper (Stationary) Glass Clamp Assembly

Upper (Stationary) Glass Clamp AsUpper (Stationary) Glass Clamp As

sembly ((((Figure 2-1A):

sembly sembly

A): This assembly clamps one end of

A):A): the capillary glass above the filament. This clamp is loosened for repositioning the glass when doing multi-step pulling.

Filament Assembly

Filament Assembly (

Filament AssemblyFilament Assembly

(Figure 2-1B):

( (

B): Your P-30 was assembled with one of two heat filament

B):B): configurations; platinum/iridium foil or nichrome coil. The platinum/iridium assembly consists of two brass clamping blocks and two black nylon thumb screws which secure the filament between spring loaded clamps. The nichrome assembly consists of a series of brass bus bars and posts for conducting current to the filament. Brass thumb screws secure the filament to the conducting posts.

Lower (Moving) Glass Clamp Assembly

Lower (Moving) Glass Clamp Assembly (

Lower (Moving) Glass Clamp AssemblyLower (Moving) Glass Clamp Assembly

(Figure 2-1C):

( (

C): This assembly clamps the lower end

C):C):

of the glass capillary, and is attached to the upper end of the slide bar.

Slide Bar

Slide Bar (

Slide BarSlide Bar

(Figure 2-1D):

( (

D): The slide bar is a hardened steel tube that guides the descent of the

D):D): lower glass clamp during pipette pulling. The weight of this bar and its attachments are resposible for the pulling force during the initial(gravity) phase of pulling.

Bushing Blocks and Guide Bushings

Bushing Blocks and Guide Bushings ((((Figure 2-1E):

Bushing Blocks and Guide Bushings Bushing Blocks and Guide Bushings

E): The bushing blocks contain the precision

E):E):

linear guide bearings that form the descent path for the slide bar.

Trip Flag Holder

Trip Flag Holder (

Trip Flag HolderTrip Flag Holder

(Figure 2-1F):

( (

F): The trip flag holder is located on the slide bar. It is held in

F):F): place by a thumb screw, its position being dependent on the type of pipette being pulled. The trip flag extends to the left of the slide mechanism so that it may interrupt the light path of the optical switch, which is located on the end of the trip point micrometer. The flag interrupts or “trips” the optical switch as it passes through the swith on its descent. The trip

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

point is adjusted by turning the trip point micrometer. Turning the micrometer clockwise increases the length of the weak pull, indicated by a decrease in the reading on the micrometer.

Guide Flag Holder

Guide Flag Holder (

Guide Flag HolderGuide Flag Holder

(Figure 2-1G):

( (

G): The guide flag holder is attached to the lower end of the

G):G): slide bar. The attached flag extends back into a slot in the cabinet and serves to prevent rotation of the slide bar and associated parts during pipette pulling. The guide flag holder is also the point of attachement for the pull solenoid. The patch block provides two adjustable spacers used in forming patch pipettes.

2.3.2

2.3.2 Pull Solenoid

Pull Solenoid

2.3.22.3.2

Pull SolenoidPull Solenoid

The pull solenoid (located just below the slide assembly in the angled portion of the cabinet) is responsible for accelerating the slide bar during the second (solenoid) phase of pipette formation. It is energized when the trip flag interrupts the optical switch as described above.

2.3.3

2.3.3 Patch Block (

Patch Block (Figure

2.3.32.3.3

Patch Block (Patch Block (

Figure 4444----1111))))

Figure Figure

The patch block is a metal block with screws on the top and bottom sides. The patch block provides two adjustable spacers used in forming patch pipettes.

P-30 MICROPIPETTE PULLER OPERATION MANUAL – REV. 3.10 (20100120)

+ 25 hidden pages

SUTTER INSTRUMENT P-30 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual