SUTTER INSTRUMENT MPC-365 User Manual

OPERATION MANUAL

Rev. 1.01 ( 20071128)

MPC

MPC----365

MPCMPC

Narrow

Narrow----Format

NarrowNarrow

Micromanipulator

Micromanipulator

MicromanipulatorMicromanipulator

System

System

SystemSystem

365

365365

Format

FormatFormat

MP

MP----365

MPMP

365

365365

Narrow-Format

Micromanipulator System

Operation Manual

(Rev. 1.01 (20071128))

Sutter Instrument Company

One Digital Drive

Novato, CA 94949

voice: 415-883-0128 web: www.sutter.com
fax: [phone] email: [e-mail]

II



Copyright © 2007 Sutter Instrument Company. All Rights Reserved.

MPC365 NARROW-FORMAT MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.01 (20071128)

III

TABLE OF CONTENTS

TABLE OF CONTENTS

TABLE OF CONTENTSTABLE OF CONTENTS

1. INTRODUCTION .................................................................................................................................................................... 1

1.1 S

TRUCTURE OF THE

1.2 C

OMPONENTS OF THE

2. MPC-200 MULTI-MANIPULATOR CONTROLLER AND ROE-200 INPUT DEVICE OPERATIONS ............ 3

2.1 E

LECTRICAL CONNECTIONS AND INITIAL OPERATING INSTRUCTIONS

2.2 I

NITIAL OPERATING INSTRUCTIONS

2.3 M

AIN CONTROLS ON THE

2.3.1 White Buttons: ...............................................................................................................................................................4

2.3.2 Black Selector Switches: ...............................................................................................................................................6

2.3.3 Other Controls on the ROE-200...................................................................................................................................7

2.4 C

ONTROLS ON THE

3. MP-265/M MANIPULATOR MECHANICAL MOUNTING INSTRUCTIONS......................................................13

3.1 M

OUNTING

3.2 S

ETTING HEADSTAGE/PIPETTE ANGLE AND PIPETTE EXCHANGE

3.3 H

EADSTAGE MOUNTING

3.4 O

THER ACCESSORIES

3.5 M

INIMIZING ELECTRICAL NOISE

3.6 I

NSTRUCTIONS USED IN SPECIAL INSTALLATIONS ONLY

3.6.1 Installing and Using the Right-Angle Adapter (285300)...........................................................................................17

3.6.2 Instructions for Changing Handedness......................................................................................................................18

APPENDIX A. FUSE REPLACEMENT ...............................................................................................................................19

APPENDIX B. TECHNICAL SPECIFICATIONS..............................................................................................................21

MP-265/M

MPC-365 D

MPC-365...........................................................................................................................................1

MPC-200...............................................................................................................................................9

TO THE

..........................................................................................................................................................17

OCUMENTATION PACKAGE

......................................................................................................................................3

ROE-200.....................................................................................................................................4

MT-72 LS........................................................................................................................13

.....................................................................................................................................................16

........................................................................................................................................17

..............................................................................................1

...............................................................................3

.....................................................................................16

..................................................................................................17

MP-265 M
MPC-200 C
ROE-200 I

INDEX ..........................................................................................................................................................................................23

ICROMANIPULATOR MECHANICAL

ONTROLLER

NPUT DEVICE

...........................................................................................................................................................21

...........................................................................................................................................................21

.........................................................................................................................21

MPC365 NARROW-FORMAT MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.01 (20071128)

1

1.

1. IN TRO DUC TI ON

INT RODU CTI ON

1.1.

INT RODU CTI ONINT RODU CTI ON

1.1

1.1 Structure of the MPC

Structure of the MPC----365 Documentation Package

1.11.1

Structure of the MPCStructure of the MPC

The MPC-365 is a manipulator system comprised of the MPC-200 controller, the ROE-200
input device and the MP-265/M narrow format stepper motor manipulator. The manual
consists of two parts, "Operations" that describes the functions of the MPC-200 controller
and ROE input device and "Setup" that describes how to install the MP-265/M mechanical
micromanipulator.

1.2

1.2 Components of the MPC

Components of the MPC----365

1.21.2

Components of the MPCComponents of the MPC

Carefully remove all components from the shipping container. In addition to this manual, the
following should be included:

• MPC-200 controller

• ROE-200 Rotary Optical Encoder input device

• MP-265/M narrow format manipulator mechanical

• Compact DB 26 to DB-25 cable (connects MP-265/M to the controller).

• RJ-45 cable (8 conductor) connects the ROE to the controller

• USB cable for computer control of the MPC-365 system

• Power cable (for the MPC-200 controller) appropriate for your location

• 2.5mm hex wrench for removing the shipping screws

• 1.5mm hex wrench for adjusting pipette angle

• MT-72 LS long slide on tower gantry for mounting the MP-265/M. Included is an MT-

7004 levered clamp for locking the MP-265/M’s mounting mechanism at any point along
the horizontal slide that is mounted atop the vertical tower gantry.

365 Documentation Package

365 Documentation Package 365 Documentation Package

365

365365

IMPORTANT

IMPORTANT

IMPORTANT IMPORTANT

Once you have unpacked your MPC

Once you have unpacked your MPC----365, remove the two shipping screws, indicated by

Once you have unpacked your MPCOnce you have unpacked your MPC
red warning tags, from the MP

red warning tags, from the MP----265/M. You must remove these screws before operating the

red warning tags, from the MPred warning tags, from the MP
manipulator. Save the screws, warning tags, and hex wrench in the event you need to

manipulator. Save the screws, warning tags, and hex wrench in the event you need to

manipulator. Save the screws, warning tags, and hex wrench in the event you need to manipulator. Save the screws, warning tags, and hex wrench in the event you need to
transport your manipulator in the future. Once these screws have been removed, ha

transport your manipulator in the future. Once these screws have been removed, handle the

transport your manipulator in the future. Once these screws have been removed, hatransport your manipulator in the future. Once these screws have been removed, ha
micromanipulator with care. The mechanism can be damaged if the axes are moved with the

micromanipulator with care. The mechanism can be damaged if the axes are moved with the

micromanipulator with care. The mechanism can be damaged if the axes are moved with the micromanipulator with care. The mechanism can be damaged if the axes are moved with the
screws in place.

screws in place.

screws in place.screws in place.

MPC365 NARROW-FORMAT MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.01 (20071128)

365, remove the two shipping screws, indicated by the

365, remove the two shipping screws, indicated by 365, remove the two shipping screws, indicated by

265/M. You must remove these screws before operating the

265/M. You must remove these screws before operating the 265/M. You must remove these screws before operating the

the

the the

ndle the

ndle the ndle the

3

MANIPULATORS

CONTROLLER

FUSE HOLDER

2.

2. M PC

MPC ----2 00 M ULTI

2.2.

MPCMPC

INP UT D EVI CE O PERA TI

INP UT D EVI CE O PERA TIO NS

INP UT D EVI CE O PERA TIINP UT D EVI CE O PERA TI

2.1

2.1 Electrical Connections and Initial Operating Instructions

Electrical Connections and Initial Operating Instructions

2.12.1

Electrical Connections and Initial Operating InstructionsElectrical Connections and Initial Operating Instructions

Initially, you may want to simply connect your two manipulators, the controller, and the
ROE together and try some gross movements in order to get a feel for the controls and how
to make simple movements. It is perfectly acceptable to set the manipulators in the middle
of a bench top, make all electrical connections and then observe each unit’s movement by
eye. Even if you wish to directly install the manipulators in your rig, it is useful to follow the
initial setup procedure to learn how to move the units to allow easy access to the mounting
screws.

1. Connect the power cord to the power entry module on the back of the MPC-200
controller.

200 MUL TI----M ANIP U

200 MUL TI200 MUL TI

POWER CORD

MAN IPU LAT OR C ONTR OLLE R A ND

MAN IPUMAN IPU

LAT OR C ONT RO LLE R AN D R OE

LAT OR C ONT RO LLE R AN DLAT OR C ONT RO LLE R AN D

ONS

ONSONS

RO E----2 00

RO E RO E

200

200 200

CONNECT

DAISY CHAIN SECOND

2. With the power OFF (front panel switch in the “0” position), connect the ROE-200 input
box to the MPC-200 controller using the RJ-45 8-conductor cable. **** Use the
CONTROLLER output on the back of the ROE and the COMMAND INPUT on the back
of the controller.

3. With the power OFF, run a DB-25-to-DB26 HD cable from each of the two MP-265/M
mechanicals to the DB-25 connectors marked “MANIPULATOR A” and
“MANIPULATOR B” on the back of the controller. ****

*NOTE: Never connect or dis

*NOTE: Never connect or disconnect the ROE or the MP

*NOTE: Never connect or dis*NOTE: Never connect or dis
controller while the power is on!

controller while the power is on!

controller while the power is on!controller while the power is on!

2.2

2.2 Initial Operating Instructions

Initial Operating Instructions

2.22.2

Initial Operating InstructionsInitial Operating Instructions

CONNECT ROE-200

Figure 2-1. Rear of MPC-200 controller cabinet..

connect the ROE or the MP----265/M to and from the MPC

connect the ROE or the MPconnect the ROE or the MP

265/M to and from the MPC----200

265/M to and from the MPC265/M to and from the MPC

200

200 200

After all electrical connections have been made, you can power up the MPC-365 using the 0/I
switch on the front of the controller. As it initializes, you will see a start up screen on the
ROE-200 that briefly displays the name of the device and the version of the installed
firmware. As the power switch is the only control you will need to access on the MPC-200,
the controller can ultimately be placed in an out of the way location (e.g., under your bench).

Once the start-up sequence has finished, you will see a display that gives the coordinates of
the connected manipulator. The LED marked 1 will light if you connected the MP-265/M to

MPC365 NARROW-FORMAT MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.01 (20071128)

4

the connector marked MANIPULATOR A. Confirm that you get a coordinate display and
have removed the shipping screws. If you do not get a coordinate display, go to the trouble
shooting section at the back of the manual. If you have not yet removed the six shipping
screws, turn the power back off and remove the six screws.

All functions necessary during normal operation are provided by four push buttons and two
rocker switches on the top of the ROE-200. Other setup functions are done via buttons and
DIP switches located on the back of the ROE-200 and DIP switches on the back of the MPC­200 controller.

The three ROE knobs control the three axes of the manipulator (right knob X, left knob Y,
and top knob Z, see Page 5). Turn any one of the three knobs and notice that the
corresponding axis moves and the coordinate for that axis changes.

The MPC-200 controller and ROE-200 have a built in Centering function. This is activated
by pressing the white “CENTER” button on the back of the ROE. If the MP-265/M
manipulator is sitting in a wide-open area, and the shipping screws are removed, press the
CENTER button. The ROE-200 display will display the message “PLEASE WAIT MOVE IN
PROGRESS”. After the CENTER operation is complete, the manipulator axes will each be
at the center of travel and the display will read 12500 for X and Z, and 6250 for Y.

From this location, you can move 12500 microns in each direction on the X- and Z-axis, and
6250 on the Y-axis. The unit will stop automatically at each end of travel (00000 or 25000
microns for X and Z, and 00000 or 12500 for Y). These ends are determined by firmware.
Each axis also have magnetic end of travel switches that are not activated in normal
operation. If the magnetic switches are activated, you will see the message EOT (for End Of
Travel) on one of the displayed axes.

When the MPC-200 controller is first turned on, the speed of movement is at its fastest,
coarsest Mode. Movement mode can be finer and slower by changing the black “Mode”
toggle switch. As MODE increases from 0, smaller movements are commanded by the same
turn of the ROE knob. MODE 5 or 6 is probably what you will use for the final approach to a
cell. MODE 0 or “Accelerated Mode” is used for fast movements to move the pipette large
distances. In MODE 0, when the ROE knob is turned slowly, a relatively slow movement is
achieved that is useful for final moves to place a pipette near a cell. Conversely, when you
make prolonged, rapid turns of the ROE knob, the controller/ROE automatically accelerates
to maximum speed to allow for prolonged, long distance movements. This would be most
useful for manual pipette exchange.

The remaining functions of the ROE are explained in the next section.

2.3

2.3 Main Controls on the ROE

Main Controls on the ROE----200

2.32.3

Main Controls on the ROEMain Controls on the ROE

2.3.1

2.3.1 White Buttons:

White Buttons:

2.3.12.3.1

White Buttons:White Buttons:

DIAG/NORM:

DIAG/NORM: Pressing the DIAG/NORM button will cause the green LED near the button to

DIAG/NORM: DIAG/NORM:

200

200200

light, indicating the MPC-200/ROE-200 is in Diagonal mode. In this mode, rotation of the Z­axis knob produces diagonal movement. A second press will put the manipulator back into
Normal mode. When in diagonal mode, the X and Y knobs remain active, allowing you to
readjust the X and Y positioning of the pipette as you approach a cell in diagonal mode. Angle
of diagonal mode movement is set via DIP switches on the back of the MPC-200 controller.
(See Controller DIP switch setting instructions on Pages 9 through10). When using MODE 9

MPC365 NARROW-FORMAT MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.01 (20071128)