Sutter Instrument MP-225 Operation Manual
MP-225
Motorized Micromanipulator
Operation Manual
Rev. 1.70 ( 20150316)
System
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax: 415-883-0572 Email: info@sutter.com
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Copyright © 2015 Sutter Instrument Company. All Rights Reserved.
MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
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DISLAIMER
The MP-225 Micromanipulator is designed for the specific use of moving micropipettes in
three-dimensional space in increments of micrometers into and out of an optical pathway of a
microscope and no other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended for use,
nor should it be used, in human experimentation or applied to humans in any way. This is
not a medical device.
Do not open or attempt to repair the instrument. Extreme heat and high voltages are
present and could cause injury.
Do not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
• Operate the MP-225 using 110-120 V AC, 60 Hz, or 220-240 V AC., 50 Hz line voltage.
This instrument is designed for connection to a standard laboratory power outlet
(Overvoltage Category II), and because it is a microprocessor--controlled device, it should
be accorded the same system wiring precautions as any 'computer type' system. A surge
protector and power regulator are recommended.
• Fuse Replacement: Replace fuse only with the same type and rating as indicated in
the following table.
Mains Voltage Setting
(Type: Time Delay, 5mm x 20mm, glass tube)
Rating Manufacturer Examples
“110” (100 – 120 VAC) 2A, 250V (Time
Delay)
“220” (200 – 240 VAC) T1.0A, 250V Bussmann: GDC-1A or S506-1A (RoHS)
Bussmann: GMC-2A, GMC-2-R (RoHS),
GDC-2A, or S506-2A (RoHS)
Littelfuse: 239 002 or 239.002.P (RoHS)
Littelfuse: 218 001 or 218 001.P (RoHS)
Fuse
A spare fuse is located in the power input module. Please refer to the fuse-replacement
appendix for more details on fuse ratings and for instructions on how to change the fuse.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power supply cord set provided to connect the system to a
grounded outlet (3-prong). This is required to protect you from injury in the event that
an electrical hazard occurs.
Do not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
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Electromagnetic Interference
To comply with FDA and CE electromagnetic immunity and interference standards; and to
reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the interconnection
of controller and manipulator mechanical and controller and ROE (see the TECHNICAL
SPECIFICATIONS appendix for more details).
Operational
Failure to comply with any of the following precautions may damage this device.
This instrument is designed for operation in a laboratory environment (Pollution Degree
I) that is free from mechanical vibrations, electrical noise and transients.
This unit is not designed for operation at altitudes above 2000 meters nor was it tested
for safety above 2000 meters.
Before making electrical connections, ensure that the instrument is switched off.
DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do this may result in damage to the electronics.
Operate this instrument only according to the instructions included in this manual.
Do not operate if there is any obvious damage to any part of the instrument.
Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
• Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and if undertaken is done so at the
users’ own risk.
• Do not attempt to operate the instrument with the manipulator shipping screws in
place - severe motor damage may result.
• Do not operate if there is any obvious damage to any part of the instrument. Do not
attempt to operate the instrument with the manipulator shipping screws in place - severe
motor damage may result. When transporting the mechanical manipulator, be sure to
install the shipping screws supplied in their correct locations. Failure to do this may
result in damage to the motors.
• Never touch any part of the micromanipulator electromechanical device while it is in
operation and moving. Doing so can result in physical injury (e.g., fingers can be caught
and pinched between the moving parts of the micromanipulator).
MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
• As with all microinjection devices, sharp micropipettes can fly out of their holder
unexpectedly. Always take precautions to prevent this from happening. Never loosen the
micropipette holder chuck when the tubing is pressurized, and never point micropipette
holders at yourself or others. Always wear safety glasses when using sharp glass
micropipettes with pressure microinjectors.
• Do not handle the manipulator mechanical while the power is on, and take care to
ensure no cables pass close to the mechanical manipulator.
Other
Use this instrument only for microinjection purposes in conjunction with the procedures
and guidelines in this manual.
Retain the original packaging for future transport of the instrument.
Some applications, such as piezo-impact microinjection call for the use of mercury in the
micropipette tip. The use of any hazardous materials with any Sutter Instrument’s
instrument is not recommended and if undertaken is done so at the users’ own risk.
When transporting the mechanical manipulator, be sure to install the shipping screws
supplied in their correct locations. Failure to do this may result in damage to the motors.
This instrument contains no user-serviceable components — do not open the instrument
casing. This instrument should be serviced and repaired only by Sutter Instrument or an
authorized Sutter Instrument servicing agent.
Sutter Instrument reserves the right to change specifications without prior notice.
This device is intended only for research purposes.
Handling Micropipettes
Failure to comply with any of the following precautions may result in injury to the users
of this device as well as those working in the general area near the device.
The micropipettes used with this instrument are very sharp and relatively fragile.
Contact with the pulled micropipette tips, therefore, should be avoided to prevent
accidentally impaling yourself.
Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps”
container.
MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
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MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
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TABLE OF CONTENTS
DISLAIMER.............................................................................................................................................3
SAFETY WARNINGS AND PRECAUTIONS ....................................................................................iii
Electrical .................................................................................................................................................iii
Avoiding Electrical Shock and Fire-related Injury.............................................................................iii
Electromagnetic Interference ...............................................................................................................iv
Operational .............................................................................................................................................iv
Other ........................................................................................................................................................v
Handling Micropipettes.......................................................................................................................... v
1. GENERAL INFORMATION..............................................................................................................1
1.1 Introduction....................................................................................................................................... 1
1.2 About This Manual........................................................................................................................... 1
1.3 Technical Support.............................................................................................................................1
2. INSTALLATION.................................................................................................................................3
2.1 Unpacking.......................................................................................................................................... 3
2.2 Mounting Instructions .....................................................................................................................3
2.2.1 Modular Construction ...............................................................................................................3
2.3 Electrical Connections......................................................................................................................5
2.4 Instructions Used in Special Installations Only............................................................................6
2.4.1 Installing and Using the Right Angle Adapter (2852RA)......................................................6
2.5 Configuration Settings .....................................................................................................................6
2.5.1 Configuring Angle of Diagonal Mode Movement ...................................................................6
2.5.2 Configuring Movement Display for each Axis when turning ROE Knob Clockwise..........7
2.5.3 Configuring the Inclusion of Y-Axis in Home and Work Pos. Robotic Moves..................... 7
3. OPERATING INSTRUCTIONS........................................................................................................9
4. INSTRUCTIONS FOR CHANGING HANDEDNESS..................................................................11
APPENDIX A. LIMITED WARRANTY ..............................................................................................13
APPENDIX B. ACCESSORIES............................................................................................................15
APPENDIX C. FUSE REPLACEMENT.............................................................................................17
APPENDIX D. TECHNICAL SPECIFICATIONS.............................................................................19
MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
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TABLE OF FIGURES
Figure 2-1. Position of locking setscrews on the front of the MP-225/M. ............................................4
Figure 4-1. Changing manipulator handedness.................................................................................... 11
Figure C-1. Power entry module............................................................................................................ 17
Figure C-2. Fuse holder........................................................................................................................... 17
TABLE OF TABLES
Table 2-1. Configuring the Angle of Diagonal-Mode Movements. ........................................................ 7
Table 2-2. Configuring Movement Display for Each Axis’ ROE Knob Clockwise Turn..................... 7
Table 2-3. Configuring the inclusion of Y-axis in Home and Work Pos. robotic moves. ....................8
Table C-1. Fuse type and rating..............................................................................................................18
Table D-1. Mains fuse type and rating. .................................................................................................. 20
Table D-2. MP-225 Controller cables and receptacles/connectors. ..................................................... 20
MP-225 MOTORIZED MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 1.70 (20150316)
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