Lambda 10----3333 is an optical filter switcher and shutter controller designed for the specific
Lambda 10Lambda 10
use of changing filters into and out of an optical pathway and no other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended, nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Do not open or attempt to repair the instrument. Extreme heat and high voltages are present
and could cause injury.
Do not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFE TY W ARNI N GS A ND
SAFE TY W ARNI N GS A ND PREC AUTI ONS
SAFE TY W ARNI N GS A ND SAFE TY W ARNI N GS A ND
Electrical
Electrical
ElectricalElectrical
PRECA UTIO NS
PRECA UTIO NSPRECA UTIO NS
Operate the Lambda 10
Operate the Lambda 10----3 using 110
Operate the Lambda 10Operate the Lambda 10
voltage.
voltage. This instrument is designed for connection to a standard laboratory power outle
voltage. voltage.
(Overvoltage Category II), and because it is a microprocessor
(Overvoltage Category II), and because it is a microprocessor--
(Overvoltage Category II), and because it is a microprocessor(Overvoltage Category II), and because it is a microprocessor
be accorded the same system wiring precautions as any 'computer type' system. A surge
be accorded the same system wiring precautions as any 'computer type' system. A surge
be accorded the same system wiring precautions as any 'computer type' system. A surge be accorded the same system wiring precautions as any 'computer type' system. A surge
protector and power regulator are recommended.
protector and power regulator are recommended.
protector and power regulator are recommended.protector and power regulator are recommended.
Fuse Replacement:
A spare fuse is located in the power input module. Please refer to the fuse
A spare fuse is located in the power input module. Please refer to the fuse----replacement
A spare fuse is located in the power input module. Please refer to the fuseA spare fuse is located in the power input module. Please refer to the fuse
appendix for more details on fuse ratings and for instructions on how to change the fuse.
appendix for more details on fuse ratings and for instructions on how to change the fuse.
appendix for more details on fuse ratings and for instructions on how to change the fuse. appendix for more details on fuse ratings and for instructions on how to change the fuse.
Avoiding Electrical Shock and Fire
Avoiding Electrical Shock and Fire----related Injury
Avoiding Electrical Shock and FireAvoiding Electrical Shock and Fire
grounded outlet (3
grounded outlet (3----prong). This is required to protect you from injury in the event th
grounded outlet (3grounded outlet (3
an electrical hazard occurs.
an electrical hazard occurs.
an electrical hazard occurs.an electrical hazard occurs.
Do not disassemble the system. Refer servicing to qualified personnel.
Do not disassemble the system. Refer servicing to qualified personnel.
Do not disassemble the system. Refer servicing to qualified personnel.Do not disassemble the system. Refer servicing to qualified personnel.
This instrument is designed for connection to a standard laboratory power outlet
This instrument is designed for connection to a standard laboratory power outleThis instrument is designed for connection to a standard laboratory power outle
Fuse Replacement: Replace
Fuse Replacement: Fuse Replacement:
T3.15A, 250V,
T3.15A, 250V, 5 x 20mm, Time Delay fuse
T3.15A, 250V, T3.15A, 250V,
(Examples: Bussmann GDC
(Examples: Bussmann GDC----3.15A or S506
(Examples: Bussmann GDC(Examples: Bussmann GDC
Always use the grounded power supply cord set provided to connect the system to a
Always use the grounded power supply cord set provided to connect the system to a
Always use the grounded power supply cord set provided to connect the system to a Always use the grounded power supply cord set provided to connect the system to a
To prevent fire or shock hazard do not expose the unit to rain or moisture.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
To prevent fire or shock hazard do not expose the unit to rain or moisture. To prevent fire or shock hazard do not expose the unit to rain or moisture.
3 using 110----120 V AC, 60 Hz, or 220
3 using 1103 using 110
Replace only with the same type and rating:
Replace Replace
5 x 20mm, Time Delay fuse (IEC 60127
5 x 20mm, Time Delay fuse 5 x 20mm, Time Delay fuse
Littelfuse 218 3.15 or 218 3.15P (RoHS))
Littelfuse 218 3.15 or 218 3.15P (RoHS))
Littelfuse 218 3.15 or 218 3.15P (RoHS)) Littelfuse 218 3.15 or 218 3.15P (RoHS))
prong). This is required to protect you from injury in the event that
prong). This is required to protect you from injury in the event thprong). This is required to protect you from injury in the event th
120 V AC, 60 Hz, or 220----240 V AC., 50 Hz line
120 V AC, 60 Hz, or 220120 V AC, 60 Hz, or 220
only with the same type and rating:
only with the same type and rating: only with the same type and rating:
(IEC 60127----2, Sheet III)
(IEC 60127(IEC 60127
3.15A or S506----3333.15R (RoHS); or
3.15A or S5063.15A or S506
related Injury
related Injuryrelated Injury
240 V AC., 50 Hz line
240 V AC., 50 Hz line 240 V AC., 50 Hz line
--controlled device, it should
controlled device, it should
----
controlled device, it should controlled device, it should
2, Sheet III)
2, Sheet III)2, Sheet III)
.15R (RoHS); or
.15R (RoHS); or.15R (RoHS); or
replacement
replacement replacement
at
at at
t
t t
Optical Radiation
Optical Radiation
Optical RadiationOptical Radiation
This system is designed for use in conjunction with high
This system is designed for use in conjunction with high----intensity light sources. Failure to
This system is designed for use in conjunction with highThis system is designed for use in conjunction with high
comply with any of the following precautions may result in injury to the users of this device
comply with any of the following precautions may result in injury to the users of this device
comply with any of the following precautions may result in injury to the users of this device comply with any of the following precautions may result in injury to the users of this device
as well as those working in the general area
as well as those working in the general area near the device.
as well as those working in the general area as well as those working in the general area
To comply with FDA and CE electromagnetic immunity and interference standards; and to
To comply with FDA and CE electromagnetic immunity and interference standards; and to
To comply with FDA and CE electromagnetic immunity and interference standards; and to To comply with FDA and CE electromagnetic immunity and interference standards; and to
reduce the electromagnetic couplin
reduce the electromagnetic coupling between this and other equipment in your lab always
reduce the electromagnetic couplinreduce the electromagnetic couplin
use the type and length of interconnect cables provided with the unit for the interconnection
use the type and length of interconnect cables provided with the unit for the interconnection
use the type and length of interconnect cables provided with the unit for the interconnection use the type and length of interconnect cables provided with the unit for the interconnection
of one or more filter wheels and/or shutters, h
of one or more filter wheels and/or shutters, host computer via serial RS
of one or more filter wheels and/or shutters, hof one or more filter wheels and/or shutters, h
interface, or othe
interface, or other equipment via TTL connections
interface, or otheinterface, or othe
appendix for more details).
appendix for more details).
appendix for more details).appendix for more details).
Never look into the optical pathway of the high intensity light sources typically used
Never look into the optical pathway of the high intensity light sources typically used
Never look into the optical pathway of the high intensity light sources typically used Never look into the optical pathway of the high intensity light sources typically used
with this instrument. Doing so can cause permanent eye damage.
with this instrument. Doing so can cause permanent eye damage.
with this instrument. Doing so can cause permanent eye damage.with this instrument. Doing so can cause permanent eye damage.
The high
The high----intensity light sources typically used with this instrument also produce a
The highThe high
ssssignificant amount of heat. Direct contact with the housing of those instruments can
ignificant amount of heat. Direct contact with the housing of those instruments can
ignificant amount of heat. Direct contact with the housing of those instruments can ignificant amount of heat. Direct contact with the housing of those instruments can
cause serious burns.
cause serious burns.
cause serious burns.cause serious burns.
The SmartShutter is Not a Safety Shutter:
The SmartShutter is Not a Safety Shutter: Sutter Instrument Co.’s SmartShutter is
The SmartShutter is Not a Safety Shutter: The SmartShutter is Not a Safety Shutter:
not i
not intended to be a ‘safety shutter.’ A safety shutter usually closes automatically in the
ntended to be a ‘safety shutter.’ A safety shutter usually closes automatically in the
not inot i
ntended to be a ‘safety shutter.’ A safety shutter usually closes automatically in the ntended to be a ‘safety shutter.’ A safety shutter usually closes automatically in the
event of a power failure and is designed with the primary goal of ensuring that it will not
event of a power failure and is designed with the primary goal of ensuring that it will not
event of a power failure and is designed with the primary goal of ensuring that it will not event of a power failure and is designed with the primary goal of ensuring that it will not
allow any unintended exposure. For laser safety applications, a shutter is no
allow any unintended exposure. For laser safety applications, a shutter is normally
allow any unintended exposure. For laser safety applications, a shutter is noallow any unintended exposure. For laser safety applications, a shutter is no
designed so that no single component failure allows an unintended exposure to the laser
designed so that no single component failure allows an unintended exposure to the laser
designed so that no single component failure allows an unintended exposure to the laser designed so that no single component failure allows an unintended exposure to the laser
beam. The SmartShutter is intended for use in the controlling of light in scientific and
beam. The SmartShutter is intended for use in the controlling of light in scientific and
beam. The SmartShutter is intended for use in the controlling of light in scientific and beam. The SmartShutter is intended for use in the controlling of light in scientific and
industrial applications. The SmartShutter was designed for high performa
industrial applications. The SmartShutter was designed for high performance and
industrial applications. The SmartShutter was designed for high performaindustrial applications. The SmartShutter was designed for high performa
durability, but without certain features that would be desirable in a safety shutter
durability, but without certain features that would be desirable in a safety shutter
durability, but without certain features that would be desirable in a safety shutter durability, but without certain features that would be desirable in a safety shutter
application.
application.
application. application.
intensity light sources typically used with this instrument also produce a
intensity light sources typically used with this instrument also produce a intensity light sources typically used with this instrument also produce a
Sutter Instrument Co.’s SmartShutter is
Sutter Instrument Co.’s SmartShutter is Sutter Instrument Co.’s SmartShutter is
rmally
rmally rmally
nce and
nce and nce and
g between this and other equipment in your lab always
g between this and other equipment in your lab always g between this and other equipment in your lab always
ost computer via serial RS----232 or parallel
ost computer via serial RSost computer via serial RS
r equipment via TTL connections (see the TECHNICAL SPECIFICATIONS
r equipment via TTL connectionsr equipment via TTL connections
(see the TECHNICAL SPECIFICATIONS
(see the TECHNICAL SPECIFICATIONS (see the TECHNICAL SPECIFICATIONS
232 or parallel
232 or parallel 232 or parallel
Operational
Operational
OperationalOperational
Failure to comply with any of the following precautions may
Failure to comply with any of the following precautions may damage this device.
Failure to comply with any of the following precautions may Failure to comply with any of the following precautions may
This instrument is designed for operation in a laboratory environment (Pollution Degree
This instrument is designed for operation in a laboratory environment (Pollution Degree
This instrument is designed for operation in a laboratory environment (Pollution Degree This instrument is designed for operation in a laboratory environment (Pollution Degree
I).
I).
I).I).
This unit is not designed for operation at altitudes above 2000 meters nor was it tested
This unit is not designed for operation at altitudes above 2000 meters nor was it tested
This unit is not designed for operation at altitudes above 2000 meters nor was it tested This unit is not designed for operation at altitudes above 2000 meters nor was it tested
for safety above 2000 meters.
for safety above 2000 meters.
for safety above 2000 meters.for safety above 2000 meters.
DO NOT CONNECT OR DISC
DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE
DO NOT CONNECT OR DISCDO NOT CONNECT OR DISC
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON. CONTROLLER AND THE MECHANICAL UNITS WHILE POWER IS ON.
ONNECT THE CABLES BETWEEN THE
ONNECT THE CABLES BETWEEN THE ONNECT THE CABLES BETWEEN THE
damage this device.
damage this device. damage this device.
Please allow at least 20 seconds after turning the unit off before disconnecting the
Please allow at least 20 seconds after turning the unit off before disconnecting the
Please allow at least 20 seconds after turning the unit off before disconnecting the Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do this may result in damage to the electronics.
mechanical units. Failure to do this may result in damage to the electronics.
mechanical units. Failure to do this may result in damage to the electronics.mechanical units. Failure to do this may result in damage to the electronics.
Oper
Operate only in a location where there is a free flow of fresh air on all sides.
ate only in a location where there is a free flow of fresh air on all sides.
OperOper
ate only in a location where there is a free flow of fresh air on all sides. ate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
3.3 Make It Go.......................................................................................................................................32
The Lambda 10-3 is a filter wheel and shutter control system designed for the rapid change
and aperture control of wavelength, and is comprised of two subsystems: 1) a controller, and
2) a combination of filter wheels and shutters that are placed in the optical pathway(s) of
your existing experimental setup. The controller is microprocessor-controlled with capability
of delivering power and control signals to one to two filter wheels, and optionally to one to
two integrated shutters. Each integrated shutter may be a SmartShutter or a conventional
shutter. Each of the two filter wheel/shutter combinations may also consist of a standalone
filter wheel and a standalone SmartShutter. A third standalone filter wheel (without
integrated shutter) is also supported. In the latest revision of the Lambda 10-3, the third
standalone filter wheel may be replaced with a standalone SmartShutter.
1.1.1
1.1.1 How to use this Manual
How to use this Manual
1.1.11.1.1
How to use this ManualHow to use this Manual
Installation of the filter wheel and filters is discussed in the FILTER WHEEL section,
SmartShutter installation is discussed in the SMARTSHUTTER section, and installation and
manual operation of the controller are discussed in the CONTROLLER section. This unit
supports a SmartShutter
three filter wheels are also supported, two of which may be equipped with shutters. Finally,
the remote operation of the Lambda 10-3 through its serial and USB ports is discussed in the
REMOTE CONTROL sections.
SmartShutter or a traditional shutter. Up to two shutters are supported. Up to
SmartShutterSmartShutter
1.1.2
1.1.2 Technical Support
Technical Support
1.1.21.1.2
Technical SupportTechnical Support
Unlimited technical support is provided by Sutter Instrument Company at no charge to our
customers. Our technical support staff is available between the hours of 8:00 AM and 5:00
PM (Pacific Time) at (415) 883
1.2
1.2 General Description
General Description
1.21.2
General DescriptionGeneral Description
The Lambda 10-3 is a microprocessor controlled, high-speed filter wheel system designed for
microscopy and other applications that require rapid and accurate wavelength switching. The
Lambda 10-3 controller can control two filter wheels each with a high-speed shutter and a
third filter wheel without a shutter, and features both USB and serial RS-232 input for
remote control.
The controller is designed around an embedded microcontroller, which automatically
determines the equipment installed. The electronics for controlling a shutter are already
installed, even if you did not order that option. If you add a shutter later, there are no
additional electronics to buy, although you may need to configure the circuit board for the
size of shutter if you wish to install a UNIBLITZ shutter.
The controller can run up to three filter wheels, up to two of which can be equipped with a
shutter, or different combinations of filter wheels and standalone smart shutters, all under
remote control through a USB or serial input using the same simple protocol as the previous
Lambda 10- 2 controller with only minor differences. The USB and the serial RS-232
interfaces use the exact same remote control command set. Some important considerations
(415) 883----0128
(415) 883(415) 883
0128. You may also E-mail your queries to info@sutter.com
about using a combination of input sources (e.g., the keypad and the Serial port) are
discussed in the Remote Control section of this manual.
The following instructions are meant to help you set up the Lambda 10-3 and become
familiar with the manual mode of filter selection. Other sections of this manual contain
detailed discussions on the functionality of the controller, how filters are installed, and
setting up remote control communications.
The LAMBDA SmartShutter
LAMBDA SmartShutter is a microprocessor controlled, high-speed shutter designed for
LAMBDA SmartShutter LAMBDA SmartShutter
microscopy and other applications that require a shutter function. The Lambda 10-3
controller can control two SmartShutters. Shutters are commonly used to turn off a light
source in order to prevent photo bleaching or other photo damage. In addition, they may be
used to select between multiple light sources or light paths, such as transmitted light versus
fluorescence excitation. SmartShutters, in particular, have the ability to be placed into one of
several modes. These modes determine the way the actual shutter action works: Fast Mode,
Soft Mode, and Neutral Density Mode. Fast Mode provides for the fastest open/close action of
the shutter. Soft Mode, is slightly slower than Fast Mode, and provides for a somewhat
gradual open/close action, as well as being quieter. Neutral Density Mode allows for the
control of the aperture of the open state of the shutter – between 1 and 144 microsteps may
be selected for the Neutral Density Mode. Neutral Density Mode also provides for the control
of light intensity without affecting the wavelength.
The primary use of the shutter is as an accessory to the Lambda 10 series of filter wheels, but
it is also used as a stand-alone piece for the transmitted light arm of inverted microscopes.
The electro-mechanical shutters actuated by solenoids have been used by most optical
manufacturers for decades, and have some outstanding features. They act quickly, they can
be driven by simple electronics, and the package is quite thin. However, these units are prone
to frequent failures and usually cannot be repaired. There is effectively no warranty from the
manufacturer for the end user. Although they can produce short bursts of operation at 30
Hertz, they will overheat and fail quickly if this rate of opening is maintained. These shutters
also produce a good deal of vibration and audible noise.
1.3
1.3 Filter Wheel
Filter Wheel
1.31.3
Filter WheelFilter Wheel
1.3.1
1.3.1 Mechanical Description
Mechanical Description
1.3.11.3.1
Mechanical DescriptionMechanical Description
The basic components of the filter wheel mechanical assembly are shown in following figures.
The only components that are to be removed by the user are the drop-in or slide-in filter
holders and the plug in the loading port. At no time should the user remove the cover plate
from the wheel housing.
Figure 1-3. Lambda 10-3 filter wheel housing with SmartShutter.
1.3.2
1.3.2 Filter Wheels
Filter Wheels
1.3.21.3.2
Filter WheelsFilter Wheels
When a Lambda 10-3 Filter Wheel system is purchased, its configuration may include one
filter wheel, with or without a shutter. If the Lambda 10-3 system is purchased with a 10position 25mm (1-inch) filter wheel, you may wish to exchange the filter wheel with a 10position 32mm or a 5-position 50mm filter wheel. If the filter wheel being replaced is a 10position 32mm filter wheel equipped with a 35mm shutter (UNIBLITZ® or SmartShutter),
then Lambda 10-3 controller must be modified by Sutter Instrument Co. before said filter
wheel with shutter is connected. If the 32mm filter wheel is not equipped with a shutter, or if
installing a 50mm filter wheel, then no modification to the Lambda 10-3 controller is needed.
Note that
Note that you must
Note that Note that
you must only
you mustyou must
only use filter wheels manufactured by Sutter Instrument Company.
onlyonly
use filter wheels manufactured by Sutter Instrument Company. All
use filter wheels manufactured by Sutter Instrument Company. use filter wheels manufactured by Sutter Instrument Company.
filter wheel options are listed in Sutter Instrument’s catalogue and web site
(http://www.sutter.com), and in the following list.
The basic components of the filter wheel mechanical assembly are shown in following figures.
The only components that are to be removed by the user are the drop-in or slide-in filter
holders and the plug in the loading port. At no time should the user remove the cover plate
from the wheel housing.
The Lambda 10-3 Controller can be used with a range of filter wheels. Most of these wheels
accept both 25 mm and 1-inch diameter filters. When larger filters are required, wheels are
available that accept 32 mm and 50 mm diameter filters.
Filter wheels designed for use with 1 inch or 25 mm filters will accept filters with a thickness
that does not exceed the following dimensions:
4.5 mm (0.18 in.) for Slide
4.5 mm (0.18 in.) for Slide----in filter holders
4.5 mm (0.18 in.) for Slide4.5 mm (0.18 in.) for Slide
5.38 mm (0.21 in.) for Drop
5.38 mm (0.21 in.) for Drop----in filter holders
5.38 mm (0.21 in.) for Drop5.38 mm (0.21 in.) for Drop
9 mm (0.35 in.) for Filter cups
9 mm (0.35 in.) for Filter cups
9 mm (0.35 in.) for Filter cups9 mm (0.35 in.) for Filter cups
in filter holders
in filter holdersin filter holders
in filter holders
in filter holdersin filter holders
Filter wheels designed for use with 32 mm filters will accept filters with a thickness that does
not exceed the following dimensions:
9 mm (0.35 in.) for Filter cups
9 mm (0.35 in.) for Filter cups
9 mm (0.35 in.) for Filter cups9 mm (0.35 in.) for Filter cups
Instructions for installing filters into the filter wheel can be found in the FILTER WHEEL
chapter of this manual. Filters are not supplied by Sutter Instrument Company but filters
conforming to the above
conforming to the above specifications can be purchased from any filter manufacturer.
conforming to the above conforming to the above
1.3.4
1.3.4 Slide
Slide----in or Drop
1.3.41.3.4
in or Drop----in Filter Holders
SlideSlide
in or Dropin or Drop
Filters are not supplied by Sutter Instrument Company but filters
Filters are not supplied by Sutter Instrument Company but filters Filters are not supplied by Sutter Instrument Company but filters
specifications can be purchased from any filter manufacturer.
specifications can be purchased from any filter manufacturer.specifications can be purchased from any filter manufacturer.
in Filter Holders
in Filter Holdersin Filter Holders
Many users prefer having extra Slide-in or Drop-in filter holders for more convenient
replacement of these “fixed” filters. Only use filter holders made by Sutter Instrument
Company in the Lambda 10
Company in the Lambda 10----3 Filter Wheel.
Company in the Lambda 10Company in the Lambda 10
3 Filter Wheel. Extra filter holders for one-inch wheels are
3 Filter Wheel. 3 Filter Wheel.
Only use filter holders made by Sutter Instrument
Only use filter holders made by Sutter Instrument Only use filter holders made by Sutter Instrument
readily available from Sutter Instrument Company or their distributors and are listed as
“SLIDE-IN” and “DROP-IN” in the Sutter catalogue. At this point, there are no slide-in
filter holders for 32 mm systems, although a 32-mm Drop-in filter holder is available. In
addition, there is a fixed filter position for a heat-blocking filter in most 32-mm microscope
adapters offered by Sutter Instrument Company. Slide-in and drop-in filter holders are not
available for the 50 mm filter wheels.
1.3.5
1.3.5 Shutters
Shutters
1.3.51.3.5
ShuttersShutters
If you purchase a Lambda 10-3 Filter Wheel system without a shutter and later decide to add
one to the system, the Filter Wheel must be returned to the factory for the upgrade. The
current version of the Lambda 10-3 Filter Wheel controller can support a retrofit with a
SmartShutter or traditional shutter, without modification. The installation of a traditional
shutter may require the setting of a jumper to configure the driver circuit for the size of
shutter installed. You must
contact Sutter Instrument Company or your distributor for details
contact Sutter Instrument Company or your distributor for details.
contact Sutter Instrument Company or your distributor for detailscontact Sutter Instrument Company or your distributor for details
You must only use shutters installed by Sutter Instrument Company
You must You must
only use shutters installed by Sutter Instrument Company --
only use shutters installed by Sutter Instrument Company only use shutters installed by Sutter Instrument Company
--
----
In addition to the shutters that are integrated with filter wheels listed previously under
“Filter Wheels”, the following SmartShutters are supported by the Lambda 10-3:
IQ12
IQ12----SA
SA 12.5mm SmartShutter™ with stand-alone housing
IQ12IQ12
SASA
IQ25
IQ25----SA1
SA1 25mm SmartShutter™ with stand-alone housing
IQ25IQ25
SA1SA1
IQ25
IQ25----LS
LS 25mm SmartShutter™ with housing to fit Lambda LS
IQ25IQ25
LSLS
IQ25
IQ25----DG
DG 25mm SmartShutter™ with housing to fit Lambda DG-4/DG-5
IQ25IQ25
DGDG
IQ35
IQ35----SA
SA 35mm SmartShutter™ with stand-alone housing
IQ35IQ35
SASA
IQ50
IQ50----SA
SA 50mm SmartShutter™ with stand-alone housing
IQ50IQ50
SASA
NOTE: Where vignetting may be an issue in a 25mm optical pathway environment, a 35mm
NOTE: Where vignetting may be an issue in a 25mm optical pathway environment, a 35mm
NOTE: Where vignetting may be an issue in a 25mm optical pathway environment, a 35mm NOTE: Where vignetting may be an issue in a 25mm optical pathway environment, a 35mm
SmartShutter is recommended.
SmartShutter is recommended.
SmartShutter is recommended.SmartShutter is recommended.
An 8-32 screw-mount hole exists on the edge of the SmartShutter opposite the motor, into
which may be attached a mounting post of any desired length. When the SmartShutter is
mounted on a post using this screw-mount hole, the exact center of the shutter aperture is
precisely aligned with the vertical center of the post. Posts of various lengths are available
from Sutter Instrument Co. This post, along with posts of other dimensions, as well as post
holders and bases, are available from most laboratory equipment suppliers.
A SmartShutter can also be mounted on a Sutter Instrument Co. Filter Wheel with a special
enclosure, as well as with other Sutter Instrument Co. products, such as the Lambda LS,
Lambda DG-4, Lambda DG-5, and various Lambda 10 series Filter Wheel controllers. Please
consult with Sutter Instrument Co.’s Technical Support for the different configuration
possibilities.
1.3.7
1.3.7 Cables
Cables
1.3.71.3.7
CablesCables
A special cable is required for connecting a standalone SmartShutter to Ports A and B of the
Lambda 10-3. The CIQ-2 cable is a split cable consisting of one DB-25 connector at the main
end (for connecting to the “SmartShutter” connector A or B on the rear of the Lambda 10-3
cabinet) and a DB-9 connector at each of the split ends. One split end is shorter than the
other. When connecting a single standalone SmartShutter to the Lambda 10-3 Port A or B,
the SmartShutter connects to the DB-9 connector of the longer split, and a terminator is
required to be attached to the DB-9 connector of the shorter split.
NOTE: Dual SmartShutters connected with the CIQ
NOTE: Dual SmartShutters connected with the CIQ----2
NOTE: Dual SmartShutters connected with the CIQNOTE: Dual SmartShutters connected with the CIQ
not supported. However, the CIQ
not supported. However, the CIQ----2 cable can be used to connect a single standalone
not supported. However, the CIQnot supported. However, the CIQ
SmartShutter to the 25
SmartShutter to the 25----pin “SmartShutter” connector f
SmartShutter to the 25SmartShutter to the 25
pin “SmartShutter” connector for port bay A or B.
pin “SmartShutter” connector fpin “SmartShutter” connector f
2 cable can be used to connect a single standalone
2 cable can be used to connect a single standalone 2 cable can be used to connect a single standalone
2 cable connected to the Lambda 10
cable connected to the Lambda 10----3 is
2 2
cable connected to the Lambda 10cable connected to the Lambda 10
or port bay A or B.
or port bay A or B.or port bay A or B.
3 is
3 is 3 is
When connecting a standalone SmartShutter to a correctly-configured Port C of the Lambda
10-3, only a standard DB-9-to-DB-9 cable is required. Note that older generations of the
Lambda 10-3 do not support a shutter on Port C.
1.3.8
1.3.8 Devices fo
Devices for TTL
1.3.81.3.8
Devices foDevices fo
r TTL----Control of Shutters
r TTLr TTL
Control of Shutters
Control of ShuttersControl of Shutters
Two devices are available from Sutter Instrument for the TTL control of shutters that are
attached to the Lambda 10-3.
FSWITCH:
FSWITCH: A foot switch with BNC connector. It changes the ON/OFF state of the
FSWITCH:FSWITCH:
shutter with each foot press. The state is maintained as long as the switch
remains depressed.
FSTOGGLE:
FSTOGGLE:
FSTOGGLE:FSTOGGLE:
Adapters
Adapters
AdaptersAdapters
Like the FSWITCH, but alternates the ON/OFF state with each foot press.
Most microscopes, cameras, light sources and other optical instruments to which the Lambda
10-3 Filter Wheel may be interfaced will require some sort of adapter to link the devices.
Adapters for interfacing the Filter Wheel to most instruments are available from Sutter
Instrument Company or can be custom built by Sutter Instrument. Suitable adapters are
available from other sources, as well, but Sutter Instrument Company assumes no
available from other sources, as well, but Sutter Instrument Company assumes no
available from other sources, as well, but Sutter Instrument Company assumes no available from other sources, as well, but Sutter Instrument Company assumes no
responsibility for the performance, suitability, and safety of adapters built by the user or
responsibility for the performance, suitability, and safety of adapters built by the user or
responsibility for the performance, suitability, and safety of adapters built by the user or responsibility for the performance, suitability, and safety of adapters built by the user or
other manufacturers.
The SmartShutter is a lightweight motor-driven aluminum vane under microprocessor
control. Because of the microprocessor control, the motion of the vane can be tailored for
different roles. The vane starts and stops under motor control, eliminating the sharp jolt
typical of other fast shutters.
1.4.1
1.4.1 Step Motor Based Shutter Advantages
Step Motor Based Shutter Advantages
1.4.11.4.1
Step Motor Based Shutter AdvantagesStep Motor Based Shutter Advantages
The new step motor-based shutter is made from a handful of parts, any of which can be
replaced if it fails. The speed of opening and closing of the shutter is practically the same as
the traditional shutters, and the mechanism is more robust. The shutter blade is stopped by
action of the motor rather than by mechanical stops that might fail. Because the motion
profile of the shutter is controlled by a microprocessor, the motion can be optimized for speed
or for smoothness. The microprocessor-based controller also offers the potential for a very
adaptable controller, which could include additional functions and modes of operation. If the
electronics fail, the motor can be disconnected and the blade set open or closed by hand.
Operation does not require over-driving the windings, so it can maintain operation at up to
40 Hz continuously. The unit can operate on modest voltages, which are typically available in
the Lambda 10 series controllers. The package size is comparable to the traditional shutters
and can be customized when needed.
Another advantage of step motor-based shutter is the ability to control the aperture of
shutter blade in its opened state. This mode is called Neutral Density and is used to control
the intensity of the light coming through the opened shutter without affecting the light’s
wavelength. The neutral density aperture size of the shutter is determined by the number of
microsteps (1 through 144) specified for the motor to open the shutter.
The following instructions describe how to set up the Lambda SmartShutter and selecting
filters in manual mode. More detailed discussions on the controller functions, installing
filters, and remote communications are found in other sections of this manual.
In this mode the user selects the extent to which the shutter
opens. When used with the Sutter Instrument light guide system (LLG), this allows the
light output from the light guide to be attenuated without changing its wavelength or
spatial distribution. The value for opening can be selected in steps from 1 (no opening) to
144 (complete opening).
1.4.3
1.4.3 Special Considerations
Special Considerations
1.4.31.4.3
Special ConsiderationsSpecial Considerations
The new step motor-based SmartShutter is made from a handful of parts, any of which can
be replaced for service. The SmartShutter can open or close in about 8 msec...., and the
mechanism is robust. The shutter blade is stopped by action of the motor rather than by
mechanical stops that might fail. Because the motion profile of the shutter is controlled by a
microprocessor, the motion can be optimized for speed or for smoothness. The
microprocessor-based controller also offers additional functions and modes of operation. If
the electronics fail, the motor can be disconnected and the blade set open or closed by hand.
1.4.3.1 Mounting
When a 25 mm SmartShutter is to be mounted to a Lambda 10 filter wheel, a special version
of the SmartShutter is supplied which will attach directly to the slide-in filter housing. The
C-mount threads in the SmartShutter housing will accept the standard Sutter microscope
adapters.
When a 35 mm SmartShutter is mounted to a 32 mm Lambda 10 series filter wheel, a special
adapter threads into the filter wheel T-mount, on to which the SmartShutter is mounted.
The 35 mm SmartShutter has a receptacle for the flange on the end of this T-mount tube and
this allows the SmartShutter to mount to the flange on the end of the tube in many different
orientations. On the other side of the SmartShutter a similar rotating mount accepts an
adapter that is used to couple to our standard microscope adapters. It is possible to install a
T-mount instead, if desired.
1.4.3.2 Opening and Closing Times
The SmartShutter has a typical time to open of 8 milliseconds, from the point a complete
command or TTL signal is received. The time to close is also eight milliseconds. This is true
for both the 25 mm and the 35 mm versions operating in the fast mode. Note that the
controller will not accept new commands or TTL pulses in the FAST mode for about 12 msec
after a command has been received. The additional time is required for display changes and
other overhead.
SmartShutter is shown in the figure above. The bottom trace is a TTL signal,
the middle trace is a 25 mm SmartShutter, and the upper trace is a 35 mm SmartShutter.
Operation is slower in the ND (Neutral Density) and SOFT modes. In the SOFT mode, the
SmartShutter has a typical time to open of 60 msec. from the point a complete command or
TTL signal is received. The time to close is also 60 msec. This is true for both the 25 mm and
the 35 mm versions. In the ND mode, the time to open and the time to close will depend on
the number of steps selected. For a complete opening of 144 steps, the time to open and the
time to close will each be about 38 msec. The time required will be approximately
proportional to the number of steps, or about 2.6 msec per 10 steps.
1.4.3.3 Repetition Rates and Duty Cycle
Operation does not require over-driving the motor windings, so the motor windings are not
damaged by frequent opening and closing over long time periods. 25 mm (one inch) versions
can usually operate well at up to 20 Hz. 35 mm versions can usually operate well at up to 15
Hz. These shutters may be operated at higher rates up to 40 Hz, but there will normally be
specific frequencies in the higher ranges that will produce errors in motion of the shutter
blade. This is the result of resonances in the mechanical and electrical systems.
If the duty cycle is substantially different from 50%, errors in movement may occur at lower
frequencies. At 40 Hz, the cycle time is 25 msec., with 12.5 msec. between open and close
commands. This is close to the absolute minimum time in either the open or closed state. If
you attempt to reduce the time in either state below 12 msec, the controller will not start to
change the shutter state until the 12-msec minimum period is over.
1.5
1.5 Controller
Controller
1.51.5
ControllerController
1.5.1
1.5.1 Principles of Operation
Principles of Operation
1.5.11.5.1
Principles of OperationPrinciples of Operation
The Lambda 10-3 controller can be operated locally (manually) from the controller keypad or
remotely from a computer either through the serial, USB, or parallel port. These four
operational modes (manual, USB and serial) are functionally identical. Each allows the user
to select:
Filter Wheel
Filter position
Filter switching speed
Shutter position
Shutter mode
The remainder of this chapter provides a detailed description of the controller’s rear panel
connectors and front panel controls. Information needed to connect the power cord and filter
wheel(s) is found in the FIRST TIME USE chapter. Instructions on operation of the
controller and manual keypad control of the filter wheel(s) are presented in the MANUAL
CONTROL chapter. The REMOTE CONTROL chapter explains control of the unit from a
remote device, as well as supplying some technical details relevant to local operation.
Technical details, which are not required for operation but which may help you understand
the behavior of the unit, are found in APPENDIX A: Stepping Motor Operation.
1.5.2
1.5.2 Rear Panel Connectors
Rear Panel Connectors
1.5.21.5.2
Rear Panel ConnectorsRear Panel Connectors
All electrical connections are all made on the rear panel of the Lambda 10-3. See the FIRST
TIME USE chapter for details on connecting the power cord and filter wheel cable(s).
SmartShutter is attached to the 25----pin connector. Do not attempt to attach two filter
SmartShutter is attached to the 25SmartShutter is attached to the 25
wheels to the Wheel A set of connectors.
wheels to the Wheel A set of connectors.
wheels to the Wheel A set of connectors.wheels to the Wheel A set of connectors.
WHEEL B
WHEEL B (Gen 1
WHEEL BWHEEL B
(Gen 1 –––– 3) or B (Gen 4)
(Gen 1 (Gen 1
The connectors for WHEEL B are provided for interfacing with a second filter wheel
and/or shutter, and have the same functionality as those for WHEEL A..
WHEEL C (
WHEEL C (Gen 1
WHEEL C (WHEEL C (
Gen 1 –––– 3)
Gen 1 Gen 1
Only a filter wheel can be connected as Wheel C. The connectors for Wheel C cannot
be used for shutters (as part of the filter wheel or standalone). If a filter wheel that is
equipped with an integrated shutter is attached as Wheel C, the integrated shutter
will be non-functional.
NOTE
NOTE 1
NOTENOTE
NOTE 2: If the filter wheel is connected to the 15
NOTE 2: If the filter wheel is connected to the 15----pin connector and has an integrated
NOTE 2: If the filter wheel is connected to the 15NOTE 2: If the filter wheel is connected to the 15
UNIBLITZ shutte
UNIBLITZ shutter, the shutter will be nonfunctional.
UNIBLITZ shutteUNIBLITZ shutte
1:
: Do not attempt to attach two filter wheels to the Wheel C set of connectors.
Do not attempt to attach two filter wheels to the Wheel C set of connectors.
1 1
: :
Do not attempt to attach two filter wheels to the Wheel C set of connectors.Do not attempt to attach two filter wheels to the Wheel C set of connectors.
pin connector. Do not attempt to attach two filter
pin connector. Do not attempt to attach two filter pin connector. Do not attempt to attach two filter
3) or B (Gen 4)
3) or B (Gen 4) 3) or B (Gen 4)
3)
3) 3)
r, the shutter will be nonfunctional.
r, the shutter will be nonfunctional.r, the shutter will be nonfunctional.
pin connector and has an integrated
pin connector and has an integrated pin connector and has an integrated
NOTE 3: If the filter wheel is connected to the 25
C (
C (Gen
Gen 4444))))
C (C (
GenGen
NOTE 3: If the filter wheel is connected to the 25----pin connector and has an integrated
NOTE 3: If the filter wheel is connected to the 25NOTE 3: If the filter wheel is connected to the 25
SmartShutter, the SmartShutter will be nonfunctional.
SmartShutter, the SmartShutter will be nonfunctional.
SmartShutter, the SmartShutter will be nonfunctional.SmartShutter, the SmartShutter will be nonfunctional.
pin connector and has an integrated
pin connector and has an integrated pin connector and has an integrated
This port array is configurable to support either a standalone filter wheel or a
standalone SmartShutter.
NOTE
NOTE 1
NOTENOTE
same time, only one or the other will be functional according to how the Lambda 10
same time, only one or the other will be functional according to how the Lambda 10----3
same time, only one or the other will be functional according to how the Lambda 10same time, only one or the other will be functional according to how the Lambda 10
is configured.
is configured.
is configured.is configured.
NOTE 2: If the connected filter whe
NOTE 2: If the connected filter wheel is equipped with a UNIBLITZ shutter, the
NOTE 2: If the connected filter wheNOTE 2: If the connected filter whe
shutter will be nonfunctional.
shutter will be nonfunctional.
shutter will be nonfunctional.shutter will be nonfunctional.
NOTE 3: A standalone SmartShutter is connected to the SMARTSHUTTER
NOTE 3: A standalone SmartShutter is connected to the SMARTSHUTTER
NOTE 3: A standalone SmartShutter is connected to the SMARTSHUTTER NOTE 3: A standalone SmartShutter is connected to the SMARTSHUTTER
connector via
connector via a cable with 9
connector via connector via
1:
: If both a standalone filter wheel and a SmartShutter are connected at the
If both a standalone filter wheel and a SmartShutter are connected at the
1 1
: :
If both a standalone filter wheel and a SmartShutter are connected at the If both a standalone filter wheel and a SmartShutter are connected at the
el is equipped with a UNIBLITZ shutter, the
el is equipped with a UNIBLITZ shutter, the el is equipped with a UNIBLITZ shutter, the
a cable with 9----pin D
a cable with 9a cable with 9
pin D----SHELL connectors at each end.
pin Dpin D
SHELL connectors at each end.
SHELL connectors at each end.SHELL connectors at each end.
USB PORT:
USB PORT: This is a B type USB connector, which allows connection to a host computer
USB PORT: USB PORT:
USB port.
SERIAL PORT
SERIAL PORT: This 9 pin, DSUB connector is used for establishing a serial interface
SERIAL PORTSERIAL PORT
between the Lambda 10-3 and a remote device (computer) using the cable provided.
3
3 3
PARALLEL POR
PARALLEL PORTTTT: This 25 pin, DSUB connector is used for establishing a parallel interface
PARALLEL PORPARALLEL POR
between the Lambda 10-3 and a remote device (computer) using the cable provided.
LINE POWER
LINE POWER: This socket is used to connect the power cord to the Lambda 10-3.
LINE POWERLINE POWER
FUSE LABEL
FUSE LABEL: Information found here includes the model number of this instrument,
FUSE LABELFUSE LABEL
appropriate supply voltage, and the type of fuse required for the supply voltage.
FUSE: Fuse compartment containing the supply voltage fuse and an extra fuse. Replace
FUSEFUSE
blown fuses with a fuse of the appropriate value as given on the fuse label (back panel of the
controller) or on the TECHNICAL SPECIFICATIONS page in this manual.
1.5.3
1.5.3 Front Panel Controls
Front Panel Controls
1.5.31.5.3
Front Panel ControlsFront Panel Controls
Figure 1-13. Lambda 10-3 front panel controls.
1.5.3.1 Power Switch
This turns on the system power.
1.5.3.2 Keypad
There are 16 keys on the Lambda 10-3 keypad.
Numerical keys 0 to 9:
Numerical keys 0 to 9: These keys manually select filter positions and, via the MODE key,
Numerical keys 0 to 9:Numerical keys 0 to 9:
choose between the eight pre-set filter wheel speeds. During menu navigation, the 0 key
causes a return to the main display.
LOCAL:
LOCAL: Takes the controller off-line (for manual operation via the controller’s front-panel
LOCAL:LOCAL:
keypad and display).
ON LINE:
ON LINE: Puts the controller on-line (for remote control from a host computer connected
ON LINE:ON LINE:
via RS-232 serial or USB).
S1:
S1: Open/close Shutter A.
S1:S1:
S2:
S2: Open/close Shutter B.
S2:S2:
MODE:
MODE: Invokes a menu structure, allowing for the setting of the following:
Select among various test modes.Select among various test modes.
•Select operation modes of the SmartShutters.
Select operation modes of the SmartShutters.
Select operation modes of the SmartShutters.Select operation modes of the SmartShutters.
•Save and restore SmartShutter mode configurations.
Save and restore SmartShutter mode configurations.
Save and restore SmartShutter mode configurations.Save and restore SmartShutter mode configurations.
ENTER:
ENTER: Used to navigate menus and for the entering of values from the keypad.
ENTER:ENTER:
While a key is depressed, the controller detects its functionality. However, the controller will
not act upon the functionality indicated by a depressed key until released. A key that is
pressed while a move is in progress but released after the move is over will cause the action
associated with that key to occur normally. Keys pressed and released while a move is in
progress are ignored.
1.5.3.3 Display
When the controller is switched (powered) on, the controller homes all the attached filter
wheels and activate all the attached shutters. During this initialization process, the following
is displayed on the controller’s display.
FILTER WHEEL CONTROLLER PROGRAM V1.21
COPYRIGHT SUTTER INSTRUMENT CORP 2004
After the controller has finished initializing, the status screen is displayed.
WHEEL: A-25MM 0 1 B-NC C-NC U
SHUTR: A- OFF B-FAST OFF
The first line of the status screen indicates which of the three possible filter wheels are
connected, what their respective sizes are, and the position and speed setting of each. “NC” is
shown for a channel to which no filter wheel is attached. At the end of the first line is a
character which indicates online/offline status of the controller (online is indicated by “U” if
the USB interface is in control or “S” for the serial interface. “L” indicates local (offline)
mode, allowing for keypad control).
The second line of the status screen shows the opened/closed state of the two possible
shutters that can be attached to the controller. When an attached shutter is a SmartShutter,
its mode is also shown.
See the next chapter for a detailed description of how to control the controller locally via the
keypad and how to navigate through the various menus shown on the controller’s front-panel
display.
1.6
1.6 Options and Accessories
Options and Accessories
1.61.6
Options and AccessoriesOptions and Accessories
1.6.1
1.6.1 Supported Filter Wheel and Shutter Configurations
Supported Filter Wheel and Shutter Configurations
1.6.11.6.1
Supported Filter Wheel and Shutter ConfigurationsSupported Filter Wheel and Shutter Configurations
The Lambda 10-3 controller provides three port bays (A, B, and C) each to which can be
connected a filter wheel and/or shutter.
1. Port Bays AAAA and BBBB each supports the following:
A filter wheel with integrated shutter (SmartShutter or UNIBLITZ®2)
A standalone filter wheel (no integrated shutter)
A standalone SmartShutter (no filter wheel)
A standalone filter wheel and a standalone SmartShutter
2
UNIBLITZ is a registered trademark of Vincent Associates.
2. Port Bay CCCC supports one standalone filter wheel. No shutter support is provided. A filter
wheel with integrated shutter can be connected to Port Bay C, but the shutter will be
inoperative.
1.7
1.7 Functional Description
Functional Description
1.71.7
Functional DescriptionFunctional Description
1.7.1
1.7.1 Stepping Motor Operation
Stepping Motor Operation
1.7.11.7.1
Stepping Motor OperationStepping Motor Operation
Stepping motors are not as familiar to most people as the common DC motor, but there are
some similarities. The DC motor consists of an armature, an electromagnet mounted on a
rotating shaft, which is located inside a permanent magnet. Current is supplied to the
electromagnet through brushes that rub on contacts on the armature. When the
electromagnet is energized, the armature rotates to align the poles of the electromagnet with
the opposite poles of the permanent magnet. Of course, before this can occur, the rotation of
the armature changes the contact plates rubbing on the brushes so that the current is
reversed. This causes the poles of the electromagnet to reverse, establishing a force for
continued rotation. This switching action is called commutation.
In stepping motors, the rotating element, called a rotor, is generally a permanent magnet
while the fixed element, the stator, is the electromagnet. The key difference between
stepping motors and DC motors, however, is the method of commutation. The DC motor
commutates automatically as it rotates. Thus, the timing of the commutation is determined
by the speed of rotation, which may vary with the load or applied power. The commutation of
the stepping motor is set by external electronics, forcing the motor to rotate at a
predetermined rate. If the load is such that the motor does not have the force to produce the
correct rate of rotation, the rotation will become erratic and may even reverse.
The force exerted between two magnet poles is proportional to the square of the distance
between the poles. A motor with a single electromagnet and only two poles would exhibit
considerable loss of power when the distance between the poles of the permanent magnet and
the electromagnet was greatest. It is understandable that, in most practical DC motors, the
armature has more than 2 poles. This allows the commutation to occur over a smaller angle
of rotation, so that the active poles can always be relatively close to the poles of the
permanent magnet.
Stepping motors are also made with multiple poles on both the rotor and stator; the exact
arrangement determines the number of steps per revolution. The motor used in the Lambda
10-3 has 200 steps per revolution (1.8 degrees per step). There are usually two windings in
the stator, and reversing the current on one of the windings produces a single step of
rotation. Reversing the current on the second winding will then produce another step. If the
first winding is then reversed again, returning to its original value, a third step will result.
Finally, reversing the second winding, so that both windings are back to their original state,
will produce a fourth step. This pattern may then be repeated to continue rotation in the
same direction. Reversing the sequence produces steps of rotation in the opposite direction.
The rate and distance of rotation is determined by the rate and number of commutation
steps. As long as the current is held constant in both windings, the rotor will not rotate. This
makes the stepping motor ideal for producing fast start and stop movements. Some
limitations should be considered. Given that there are only 4 states of the control electronics
(2 polarities for each of the 2 windings) but 200 steps per revolution, it follows that, for each
of these 4 states, there are 50 possible rotary positions. In order to establish the absolute
position an external sensor must be added. Absolute position only needs to be determined
once so long as the subsequent moves occur without errors.
Correct operation of stepping motors, which is outlined above, is not always obtained. If the
motor lacks the torque to accelerate and decelerate the load at the rate indicated by the
control electronics, the resulting move may differ from that commanded by the control
electronics. For instance, if enough external force is applied, the motor shaft may be rotated
even though the command signal has not changed. For small rotations (less than 3.6
degrees), the motor shaft will return to the correct position if the force is removed. For larger
rotations, the shaft will stop at the nearest of the 50 correct positions for that command
signal when the external force is removed.