Sutter Instrument dPatch, dPatch-2 Quick Start Manual

dPatch

®

DIGITAL PATCH CLAMP AMPLIFIER SYSTEM

with

®

SutterPatch

DATA ACQUISITION, MANAGEMENT AND ANALYSIS SOFTWARE

QUICK START GUIDE

QUICK START GUIDE dPATCH

®

/ dPATCH-2

DIGITAL PATCH CLAMP AMPLIFIER SYSTEMS OVERVIEW

Please read these instructions carefully before installation. This guide covers both the
dPatch® and dPatch-2 systems. If you have any questions or need additional information,
contact Sutter Instrument.

OPERATING SYSTEM REQUIREMENTS

Windows 7 or later: 64-bit versions
Mac OS 10.11 (El Capitan) or later

INSTALL HARDWARE

1. Attach dPatch headstage(s) to amplifier front panel: HEADSTAGE 1 & 2. dPatch
headstages can be attached to any headstage port; they are not “matched” to a port.

2. OPTIONAL: Attach dPatch Screw Terminal Board or dPatch Expansion Panel to
amplifier rear panel: DIGITAL OUTPUTS.

3. Attach USB 3 cable to USB 3 port on amplifier rear panel and your computer. The
connector on the dPatch rear panel must click in place.

4. Attach power cord to amplifier rear panel and a grounded power outlet.

INSTALL IGOR PRO/SUTTERPATCH SOFTWARE

1. Power on computer.

2. If you have Internet access, download the latest version of the SutterPatch Software
installer from www.sutter.com/AMPLIFIERS/SutterPatch.html

3. If Internet access is not available, attach the included USB flash drive to your
computer.

4. Double click on ‘SutterPatch_Installer_with_Igor’ (Windows) or
‘sutterpatch_mac_full’ (Mac OS).

5. Follow the installer prompts for current Igor software.

6. Open Igor Pro and activate the Igor Pro license as instructed:

i. Serial Number:
ii. Activation Key:

affix label here

4. On dashboard window:
I. Click on the Acquire Data icon:

Acquire Data

II. Click on the Membrane Test icon:

Membrane Test

5. Scope + Analysis windows open:

(Actual analysis values depend on dPatch filter settings)

I. With the Model Cell switch in Bath position, click the Bath button.
Pipette Resistance = ~10 MΩ
II. With the Model Cell switch in Seal position, click the Seal button.
Seal Resistance > 10,000 MΩ
III. With the Model Cell switch in Cell position, click the Cell button.
Access Resistance = ~10 MΩ
Membrane Resistance = ~500 MΩ
Membrane Capacitance = ~28 pF

Move the Model Cell and shielding to HEADSTAGE 2 (if installed), set the Scope

window to ‘Headstage 2’, and repeat steps I – III.

TEST SYSTEM

1. Model Cell:
a. Attach Model Cell to HEADSTAGE 1 and tighten the collar.
b. Attach ground wire to HEADSTAGE 1 and Model Cell (gold plugs).
c. Surround with aluminum foil shielding and ground it to the headstage

ground connector.

2. Click icon to launch Igor:

3. SutterPatch welcome screen will open. Press ‘Start’.

SUTTER INSTRUMENT

BACK PANEL

DIGITAL OUTPUTS 1-16

19 1

37 20

DIGITAL OUTPUTS PINOUT

PIN FUNCTION

1 - 16 Digital Output 1 - 16

17 - 19 +5 V

20 - 35 Ground

36 - 37 +5 V

DIGITAL PATCH CLAMP AMPLIFIER SYSTEM