Sutter Instrument DG-4, DG-5 User Manual

OPERATION MANUAL

Rev. 2.14 ( 20081107)

Lambda DG

Lambda DG----4

Lambda DGLambda DG Lambda DG

Lambda DG----5

Lambda DGLambda DG

Ultra

Ultra----High

UltraUltra

Wavelength Switching

Wavelength SwitchingWavelength Switching

Systems

SystemsSystems

and

andand

High----Speed

HighHigh

Speed

SpeedSpeed

44 5

Lambda DG

Lambda DG----4 and Lambda DG

Lambda DGLambda DG

4 and Lambda DG----5555

4 and Lambda DG4 and Lambda DG

Ultra-High-Speed Wavelength Switching

Systems

Operation Manual

(Rev. 2.14 (20081107))

Sutter Instrument Company

One Digital Drive Novato, CA 94949

Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com



LAMBDA 10 ® is a registered trademark, and Sutter Instrument Company.

Smart

Shutter™ is a trademark, both of

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

TABLE OF CONTENTS

TABLE OF CONTENTSTABLE OF CONTENTS

1. GENERAL INFORMATI

1. GENERAL INFORMATION

1. GENERAL INFORMATI1. GENERAL INFORMATI

1.1 A

BOUT THIS MANUAL

1.2 L

AMBDA

1.3 U

NPACKING

2. SAFETY WARNINGS

2. SAFETY WARNINGS ................................

2. SAFETY WARNINGS2. SAFETY WARNINGS

DG-4

AND LAMBDA

...................................................................................................................................... 1

ON ................................

................................................................

ONON

................................................................

......................................................................................................................1

DG-5.................................................................................................1

................................................................

...................................................

................................................................

........................................

................................................................

........ 1111

................

................... 3333

......................................

III

2.1 TO F

3. MAKE IT GLOW

3. MAKE IT GLOW................................

3. MAKE IT GLOW3. MAKE IT GLOW

3.1 S

3.2 G

4. OPTICAL PATHWAY

4. OPTICAL PATHWAY ................................

4. OPTICAL PATHWAY4. OPTICAL PATHWAY

4.1 L

URTHER AVOID ELECTRICAL SHOCK

................................................................

OME BASIC INFORMATION

ETTING STARTED

IGHT GUIDE

..........................................................................................................................6

................................................................

& O

PTICAL HOLDER INSTALLATION

.............................................................................................................5

: ....................................................................................3

................................................................

............................................................

................................................................

...................................................

................................................................

......................................................................9

............................ 5555

........................................................

................... 9999

......................................

4.1.1 Liquid Light Guide...................................................................................................................9

4.1.2 Liquid Light Guide Installation .............................................................................................9

4.1.3 Optical Holder........................................................................................................................10

4.2 L

OADING FILTERS

..........................................................................................................................10

4.2.1 Filter Orientation...................................................................................................................10

4.2.2 Loading Filters .......................................................................................................................11

4.2.3 Selection of a Filter Position.................................................................................................12

4.2.4 Blanking Discs........................................................................................................................12

4.2.5 Neutral Density Filters .........................................................................................................12

4.2.6 Filter Holder Installation......................................................................................................13

5. CONTROLLER

5. CONTROLLER ................................

5. CONTROLLER5. CONTROLLER

5.1 C

ONTROL ELECTRONICS

5.2 P

OWER-UP SEQUENCE

5.3 T

OP PANEL CONTROLS

................................................................

...............................................................................................................15

...................................................................................................................15

.................................................................................................................16

................................................................

............................................................

................................................................

............................ 15

........................................................

5.3.1 Keypad.....................................................................................................................................16

5.4 E

LECTRICAL CONNECTIONS

.........................................................................................................16

5.4.1 Parallel Port............................................................................................................................17

5.4.2 Serial Port...............................................................................................................................17

5.4.3 Filter Value Out.....................................................................................................................17

5.4.4 Sync..........................................................................................................................................18

5.4.5 DAC..........................................................................................................................................18

5.4.6 Strobe ......................................................................................................................................18

5.4.7 Line Power..............................................................................................................................18

5.5 F

USE

...............................................................................................................................................18

5.6 M

ODES OF OPERATION

(LOCAL).................................................................................................19

5.6.1 General Information..............................................................................................................19

5.6.2 Selecting an Operational Mode: The MODE Key..............................................................20

5.6.3 Move on Command Mode......................................................................................................21

5.6.4 Move on Sync Pulse Mode.....................................................................................................23

1515

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

5.6.5 Move on Strobe Pulse Mode..................................................................................................24

5.6.6 Move on Sync, Inhibited by the Strobe Line Mode............................................................25

5.6.7 Edit a Filter Definition Mode ...............................................................................................27

5.6.7.1 General Information .................................................................................................................................................27

5.6.7.2 Filter Numbers...........................................................................................................................................................28

5.6.7.3 Attenuation.................................................................................................................................................................28

5.6.7.4 Programming a Filter Number (“Logical Filter”)\..............................................................................................29

5.6.7.5 Manual Adjustment of Attenuation .......................................................................................................................30

5.6.8 Setup Ring Buffer Mode........................................................................................................33

5.6.8.1 General Information .................................................................................................................................................33

5.6.8.2 Storing the FILTER NUMBER Sequence ............................................................................................................33

5.6.8.3 Viewing an Existing FILTER NUMBER Sequence ............................................................................................34

5.6.8.4 Running the RING BUFFER Sequence................................................................................................................35

5.6.9 Turbo-Blanking Mode ...........................................................................................................37

5.6.10 Display the First Four Filter Values Mode.......................................................................38

6. REMOTE INTERFACE

6. REMOTE INTERFACE ................................

6. REMOTE INTERFACE6. REMOTE INTERFACE

6.1 P

ARALLEL INTERFACE

................................................................

...................................................................................................................39

................................................................

..............................................

................................................................

.............. 39

............................

6.1.1 Connecting to the Lambda DG-4 parallel port...................................................................39

6.1.2 Input Lines..............................................................................................................................40

6.1.3 Command Codes.....................................................................................................................40

6.1.4 Busy Line ................................................................................................................................43

6.1.5 Interface Modes......................................................................................................................43

6.1.6 Controlling the Lambda DG-4 from a Computer’s Parallel (Printer) Port....................44

6.1.7 Reading the Status of the Lambda DG-4 ............................................................................46

6.1.8 Shutter Control ......................................................................................................................47

6.2 S

ERIAL INTERFACE

........................................................................................................................48

6.2.1 Connecting to the Lambda DG-4 Serial Input ...................................................................48

6.2.2 Controlling the Lambda DG-4 from a Computer’s Serial Port........................................49

6.2.3 Serial Port Command Echo ..................................................................................................50

6.2.4 Serial Port Command Completion Indicator......................................................................50

6.2.5 Reading the Status of the Lambda DG-4 ............................................................................50

3939

AAAAPPENDIX A. FUSE REPL

PPENDIX A. FUSE REPLACEMENT

PPENDIX A. FUSE REPLPPENDIX A. FUSE REPL

APPENDIX B. LIMITED

APPENDIX B. LIMITED WARRANTY

APPENDIX B. LIMITED APPENDIX B. LIMITED

APPENDIX C. DISCLAIM

APPENDIX C. DISCLAIMER

APPENDIX C. DISCLAIMAPPENDIX C. DISCLAIM

APPENDIX D. TECHNICA

APPENDIX D. TECHNICAL SPECIFICATIONS

APPENDIX D. TECHNICAAPPENDIX D. TECHNICA

APPENDI

APPENDIX E. REMOTE

APPENDIAPPENDI

TABLE OF FIGURES

TABLE OF FIGURESTABLE OF FIGURES

Figure 3-1. Lambda DG-4 optical pathway. ...........................................................................................5

Figure 3-2. Light guide installation..........................................................................................................6

X E. REMOTE----CONTROL

X E. REMOTEX E. REMOTE

ACEMENT ................................

ACEMENTACEMENT

WARRANTY ................................

WARRANTYWARRANTY

ER ................................

................................................................

ERER

................................................................

L SPECIFICATIONS................................

L SPECIFICATIONSL SPECIFICATIONS

CONTROL COMMAND REFERENCE

CONTROL CONTROL

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

................................................................

COMMAND REFERENCE................................

COMMAND REFERENCECOMMAND REFERENCE

.......................................................

................................................................

........................................................

................................................................

........................................

................................................................

.......................................

................................................................

............................................

................................................................

.......................53

..............................................

........................55

................................................

............61

........................

5353

5555

........ 57

................

5757

....... 59

..............

5959

6161

Figure 3-3. Lambda DG-4 left side. .........................................................................................................7

Figure 4-1. Liquid light guide installation..............................................................................................9

Figure 4-2. Light guide optical holder...................................................................................................10

Figure 4-3. Filter holder assembly.........................................................................................................11

Figure 4-4. Filter holder assembly with blanking disc........................................................................11

Figure 4-5. Lambda DG-4 left side. .......................................................................................................13

Figure 5-1. Keypad (top panel). .............................................................................................................16

Figure 5-2. Electrical connections (rear panel)....................................................................................17

Figure 5-3. Filter value out connector...................................................................................................18

Figure 5-4. Optical pathway based on filter position. .........................................................................27

Figure 5-5. DG-4 output attenuation....................................................................................................28

Figure 5-6. Log of attenuation vs. change in output galvanometer value. .......................................29

Figure 6-1. Parallel port DB25 connector (male).................................................................................39

Figure 6-2. DB25 Parallel connector.....................................................................................................45

Figure 6-3. Serial control connections (viewed from rear of cabinet). ..............................................48

Figure A-1. Fuse replacement................................................................................................................53

TABLE OF TABLES

TABLE OF TABLESTABLE OF TABLES

Table 5-1. Input and output galvanometer values based on filter position......................................27

Table 6-1. Parallel port pin assignments..............................................................................................39

Table 6-2. Command code types. ...........................................................................................................40

Table 6-3. Controller commands............................................................................................................41

Table 6-4. Parallel port pin connections and definitions....................................................................45

Table 6-5. Parallel port numbering and addressing............................................................................46

Table 6-6. Shutter open and close commands......................................................................................48

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

TableA-1. Mains fuse requirements according to lamp power ratings and line voltage.................54

Table D-1. Cable specifications...............................................................................................................60

Table E-1. Remote-control filter selection command reference..........................................................61

Table E-2. Filter selection command structure. ...................................................................................62

Table E-3. Remote-control special commands reference.....................................................................62

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

1. G ENER AL INFO RMA TION

GEN ERAL IN FOR M ATI ON

1.1.

GEN ERAL IN FOR M ATI ONGEN ERAL IN FOR M ATI ON

1.1

1.1 About this Manual

About this Manual

1.11.1

About this ManualAbout this Manual

The Lambda DG-4 is an illumination system designed for rapid change of wavelength and comprises three subsystems: controller, optical path and 175-Watt Xenon arc lamp with power supply. All three subsystems are conveniently housed in one cabinet. For the most part this manual is organized around those subsystems to provide you with ready access to information, as you need it. Separate documentation for the lamp and its power supply (provided by PerkinElmer Optoelectronics) accompanies this manual.

In the next few pages you will find information on the safe use of this device, unpacking instructions and quick-start (“Make it Glow”) directions to help you get acquainted with the operation of the Lambda DG-4 (using the manual mode). Please take the time to read these instructions to assure the safe and proper use of this instrument.

This manual is currently under construction. If there are any areas that you feel should be covered in greater detail we would like to hear from you. Please contact our Technical Support staff with your suggestions (415-883-0128 or info@sutter.com).

1.2

1.2 Lambda DG

Lambda DG----4 and Lambda DG

1.21.2

Lambda DGLambda DG

4 and Lambda DG----5555

4 and Lambda DG4 and Lambda DG

Throughout this manual, unless otherwise noted, all references and descriptions pertaining to “Lambda DG-4” (or just “DG-4”) apply equally to the Lambda DG-5. The only difference between the Lambda DG-4 and Lambda DG-5 is the set of light interference filters, as follows:

• The Lambda DG-4 filter set consists of four 25mm (1-inch) diameter filters.

• The Lambda DG-5 filter set consists of three 18mm and two 25mm (1-inch) diameter

filters.

Both models of the Lambda DG series are calibrated at the factory according to each one’s filter set arrangement.

1.3

1.3 Unpacking

Unpacking

1.31.3

UnpackingUnpacking

The Lambda DG-4 and associated hardware comes packed in a single carton. The following is a list of the components found there. If you believe that any of these components are missing or show obvious signs of damage from shipping, please contact the factory.



Parallel Interface Cable



Serial Interface Cable



Power Cord



4 Individual Filter holders with retaining rings, spacer rings and blanking discs



Neutral Density holder retaining ring, spacer ring and blanking disc



Spanner Wrench



Liquid Light Guide



Manual



Microscope adapters (if ordered)

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

2. S A FET Y W A RNI NGS

SAF ETY WAR NIN GS

2.2.

SAF ETY WAR NIN GSS AFET Y W ARN I NGS





Replace fuse only with the same type

Replace fuse only with the same type::::

Replace fuse only with the same type Replace fuse only with the same type

5 x 20 mm glass tube

5 x 20 mm glass tube5 x 20 mm glass tube TTTTime delay

ime delay (medium)

ime delayime delay

RRRRating:

ating:

ating:ating:

If this instrument is equipped with

If this instrument is equipped with a

If this instrument is equipped with If this instrument is equipped with

삟 100 삟 220

If this instrument is equipped with a

If this instrument is equipped with a 300

If this instrument is equipped with a If this instrument is equipped with a

삟 100 삟 220

A spare fuse is provided, being located in the power input module. Please refer to

A spare fuse is provided, being located in the power input module. Please refer to A spare fuse is provided, being located in the power input module. Please refer to Appendix A

Appendix A:

Appendix AAppendix A instructions on ho

instructions on how to change the fuse.

instructions on hoinstructions on ho

(medium),

(medium) (medium)

100 –––– 120 VAC Operation: 4.0A, 125V fuse; or

120 VAC Operation: 4.0A, 125V fuse; or

100 100

120 VAC Operation: 4.0A, 125V fuse; or 120 VAC Operation: 4.0A, 125V fuse; or

220 –––– 240 VAC Operation: T3.15A, 250V fuse.

240 VAC Operation: T3.15A, 250V fuse.

220 220

240 VAC Operation: T3.15A, 250V fuse. 240 VAC Operation: T3.15A, 250V fuse.

100 –––– 120 VAC Operation: 5.0A, 125V fuse, or

120 VAC Operation: 5.0A, 125V fuse, or

100 100

120 VAC Operation: 5.0A, 125V fuse, or 120 VAC Operation: 5.0A, 125V fuse, or

220 –––– 240 VAC Operation: T

240 VAC Operation: T3.15A, 250V fuse.

220 220

240 VAC Operation: T 240 VAC Operation: T

: FUSE

FUSE REPLACEMENT

: :

FUSEFUSE

w to change the fuse.

w to change the fuse.w to change the fuse.

, time lag

time lag,,,, or

, ,

time lagtime lag

REPLACEMENT for more details on fuse ratings and for

REPLACEMENTREPLACEMENT

or ““““slow blow

slow blow””””))))

or or

slow blowslow blow

a 175

175----Watt lamp

a a

3.15A, 250V fuse.

3.15A, 250V fuse.3.15A, 250V fuse.

for more details on fuse ratings and for

for more details on fuse ratings and for for more details on fuse ratings and for

Watt lamp, replace with

175175

Watt lampWatt lamp

300----Watt lamp

Watt lamp, replace with

300300

Watt lampWatt lamp

, replace with

, replace with, replace with

, replace with

, replace with, replace with



Infrared Radiation: The infrared radiation (and ultraviolet radiation) generated by

Infrared Radiation: The infrared radiation (and ultraviolet radiation) generated by Infrared Radiation: The infrared radiation (and ultraviolet radiation) generated by this lamp can cause significant skin burns and eye damage.

this lamp can cause significant skin burns and eye damage.

this lamp can cause significant skin burns and eye damage.this lamp can cause significant skin burns and eye damage.



Explosion: High internal pressure

Explosion: High internal pressure exists in any xenon arc lamp.

Explosion: High internal pressureExplosion: High internal pressure



High Voltage: High ignition voltages, which exist inside the cabinet, can be lethal.

High Voltage: High ignition voltages, which exist inside the cabinet, can be lethal.High Voltage: High ignition voltages, which exist inside the cabinet, can be lethal.



Ozone: Because the UV

Ozone: Because the UV----enhanced version of the lamp generates significant ozone, it

Ozone: Because the UVOzone: Because the UV must be used in a well

must be used in a well----ventilated environment. For mos

must be used in a wellmust be used in a well requirement to ventilate the ozone

requirement to ventilate the ozone----free lamp.

requirement to ventilate the ozonerequirement to ventilate the ozone

2.1

2.1 To Further Avoid Electrical Shock

To Further Avoid Electrical Shock and Fire

2.12.1

To Further Avoid Electrical ShockTo Further Avoid Electrical Shock



Always use the grounded power supply cord set provided to connect the unit to a

Always use the grounded power supply cord set provided to connect the unit to a Always use the grounded power supply cord set provided to connect the unit to a grounded outlet (3

grounded outlet (3----prong). This is required to protect you from injury i

grounded outlet (3grounded outlet (3 an electrical hazard occurs.

an electrical hazard occurs.

an electrical hazard occurs.an electrical hazard occurs.



Do not disassemble the unit. The only user serviceable parts are the line fuse, the

Do not disassemble the unit. The only user serviceable parts are the line fuse, the Do not disassemble the unit. The only user serviceable parts are the line fuse, the xenon bulb, and filters. The line fuse is accessible from the outside of the unit. The

xenon bulb, and filters. The line fuse is accessible from the outside of the unit. The

xenon bulb, and filters. The line fuse is accessible from the outside of the unit. The xenon bulb, and filters. The line fuse is accessible from the outside of the unit. The bulb and the filters are accessible v

bulb and the filters are accessible via separate panels on the side of the unit. Bulb

bulb and the filters are accessible vbulb and the filters are accessible v replacement and filter installation/removal are covered in separate sections of the

replacement and filter installation/removal are covered in separate sections of the

replacement and filter installation/removal are covered in separate sections of the replacement and filter installation/removal are covered in separate sections of the manual and should only be attempted with the power cord disconnected.

manual and should only be attempted with the power cord disconnected.

manual and should only be attempted with the power cord disconnected.manual and should only be attempted with the power cord disconnected.



To prevent fire or shock hazard do not expose the un

To prevent fire or shock hazard do not expose the unit to rain or moisture.

To prevent fire or shock hazard do not expose the unTo prevent fire or shock hazard do not expose the un

enhanced version of the lamp generates significant ozone, it

enhanced version of the lamp generates significant ozone, it enhanced version of the lamp generates significant ozone, it

ventilated environment. For most users, there is no

ventilated environment. For mosventilated environment. For mos

prong). This is required to protect you from injury in the event that

prong). This is required to protect you from injury iprong). This is required to protect you from injury i

exists in any xenon arc lamp.

exists in any xenon arc lamp. exists in any xenon arc lamp.

t users, there is no

t users, there is no t users, there is no

free lamp.

free lamp.free lamp.

and Fire----related

and Fire and Fire

ia separate panels on the side of the unit. Bulb

ia separate panels on the side of the unit. Bulb ia separate panels on the side of the unit. Bulb

related Injury

relatedrelated

Injury

Injury Injury

it to rain or moisture.

it to rain or moisture.it to rain or moisture.

n the event that

n the event that n the event that

Finally, the original manufacturer of the xenon lamp and power supply used in the

Finally, the original manufacturer of the xenon lamp and power supply used in the Finally, the original manufacturer of the xenon lamp and power supply used in the Lambda DG

Lambda DG----4 provides the additional safety information on the following page. It is

Lambda DGLambda DG intended to amplify the information given above.

intended to amplify the information given above.

intended to amplify the information given above.intended to amplify the information given above.

4 provides the additional safety information on the following page. It is

4 provides the additional safety information on the following page. It is 4 provides the additional safety information on the following page. It is

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

PerkinElmer Optoelectronics

44370 Christy Street

Fremont CA 94538

Phone: 510-979-6500

FAX: 510-687-1152

SHORT ARC XENON LAMPS AND SYSTEMS

Proper use and safe operating practices are the responsibility of equipment manufacturers who incorporate the lamp into equipment and users of such lamps and equipment. The supplier of this lamp provides information on its products and associated hazards, but it assumes no responsibility for aftersale operating and safety practices. All lamps are under pressure and must be handled with care. Take appropriate action through baffles, light shields, interlock switches or other safeguards to protect personnel from harm due to operation and/or failure of the lamp.

SAFE OPERATING INSTRUCTIONS

Do not operate this lamp except in accordance with proper operating instructions and within recommended operating specifications. Direct questions regarding lamp operation or safety to your lamp supplier.

OPERATING HAZARDS

Read the following instructions and take all necessary precautions

I. EXPLOSION - The lamps are filled with xenon gas at very high pressure. Lamps must be handled with the same care and caution given any vessel containing these levels of pressure. A hazard exists if the window or ceramic fractures and may cause explosive mechanical failure. Face shields or proper safety glasses are recommended during all handling operations.

II. HIGH VOLTAGE - Ignition voltage of some lamp models is very high and can be deadly. If portions of the circuit are exposed, caution must be used in setup and operation of the system. The input power must be disconnected from the power source before attempting any service to the lamp.

III. INFRARED AND ULTRAVIOLET RADIATION - Do not look directly at operating lamps orb reflected light. Infrared and ultra violet radiation generated by the lamp can cause skin burns and permanent eye damage.

CERMAX® Lamps

LAMP DISPOSAL

CERMAX lamps do not have reclaimable parts. Before disposal, it is recommended to relieve a lamp’s gas pressure by squeezing the tip-off with pliers until the gas escapes. If gas pressure is not relieved, care should be taken to discard the lamp in a landfill and not an incinerator.

SAFETY HAZARDS

The operation of lamps involves one or more of the following hazards. In the absence of safe operating practices and precautions, any one of these hazards could result in injury.

IV. OZONE - Some UV type lamps generate ozone, a toxic gas, by virtue of the ultraviolet radiation. A lamp which gives off ozone must be operated in a well ventilated area..

V. HOT SURFACES - Portions of the lamp can reach temperatures of several hundred degrees centigrade and cause serious burns if touched even after the lamp is turned off.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

3. MA KE IT G LOW

MAK E IT GLO W

3.3.

MAK E IT GLO WMA K E I T GL OW

3.1

3.1 Some Basic Information

Some Basic Information

3.13.1

Some Basic InformationSome Basic Information

The optical path subsystem in the Lambda DG-4 is arranged so that 2 mirrors, each mounted on a fast closed-loop servoed galvanometer, can route the collimated output of the lamp through one of 4 optical paths (e.g., filter position 1 in the diagram below). Slide-in filter holders allow 25 mm (or 1 inch) diameter filters to be introduced in each of the four optical paths. An additional slide-in filter holder, intended for neutral density filters, is located in a portion of the optical system common to all light paths. After passing through the filter positions, the lamp output is focused on a 2-meter long UV (ultraviolet) transmitting light guide, which acts as a conduit for the output to the microscope. At the output of the light guide, an optical adapter collects the output and delivers it to the microscope.

Figure 3-1. Lambda DG-4 optical pathway.

The following instructions are meant to help you set up the Lambda DG-4 and verify that the Lambda DG-4’s subsystems are operational using the manual mode of filter selection. More detailed discussions of controller function, filter installation, the light source, assignment of filter numbers and remote communications can be found in other sections of this manual.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

3.2

3.2 Getting Started

Getting Started

3.23.2

Getting StartedGetting Started

1. Connect the power cord to the socket on the back panel of the Lambda DG-4. Make certain that the voltage selector next to the power cord socket has been properly preset to the appropriate voltage (110 or 220). Do not turn on the power, yet.

2. Insert either end of the light guide into the port on the top panel of the Lambda DG-4 cabinet (see below) and tighten the thumbscrew to hold it in place. The top of the light guide end sleeve should be flush with the top surface of the port fitting (see below). This should result in maximal or near-maximal light output from the light guide. Minor adjustments up from this position may improve output, and can be easily made once the system is fully installed.

Figure 3-2. Light guide installation.

3. The other end of the light guide should be installed in the microscope using the appropriate adapters (see the installation sheet provided with the specific adapter that has been ordered) or directed away from anyone’s eyes and toward a non-reflective surface. DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE!

4. A hose should be connected to the 4” fitting on the back of the Lambda DG-4 cabinet to ventilate the ozone from the xenon lamp to the environment or to an ozone removal device.

5. Identify the small access door used to install the neutral density FILTER HOLDER. This access door is located near the top edge of the left side panel on the Lambda DG-4 cabinet (see below). Loosen the black knob on the access door and slide it up to install the empty Neutral Density FILTER HOLDER (the neutral density FILTER HOLDER is the one whose bottom surface is flat rather than grooved). Make certain that the end nearest the filter cup goes in first. If you would like to install a neutral density filter at this time please refer to the “Loading Filters” section of this manual.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

Figure 3-3. Lambda DG-4 left side.

6. Identify the large access door used to install the interference FILTER HOLDERS. This access door is located in the middle of the left side panel on the Lambda DG-4 cabinet (see above). Loosen the black knob on the access door and slide it up to install the remaining four FILTER HOLDERS (the ones that have a groove on their bottom surface). Install the two FILTER HOLDERS that contain blanking disks in FILTER POSITIONS 3 and 4. If you would like to install interference filters at this time, please refer to the “Loading Filters” section of this manual.

7. Make certain that the free end of the light guide is installed into the microscope or is directed toward a non-reflective surface and away from anyone’s eyes. DO NOT LOOK DIRECTLY INTO THE LIGHT GUIDE.

8. Turn on the power to the lamp subsystem using the switch labeled LAMP on the back panel of the Lambda DG-4 cabinet. Due to the current draw during lamp ignition, it is important to power up the LAMP first (before the MAIN switch is turned on) to avoid damage to the circuitry of the other Lambda DG-4 subsystems. This same precaution applies to the order in which you power up other electronic and computer equipment that might be powered from or grounded to the same circuit as the one used for the Lambda DG-4. GO DIRECTLY TO THE NEXT STEP! DO NOT OPERATE THE LAMP FOR MORE THAN A FEW SECONDS WITHOUT TURNING ON THE MAIN POWER SWITCH!

9. Within a few seconds of turning on the LAMP switch turn on the power to the optical path and controller subsystems using the switch labeled MAIN on the back panel of the

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

Lambda DG-4 cabinet. The Lambda DG-4 will boot up and briefly display the initialization screen followed by the MAIN MENU (shown on top of next page).

OFF= FILTER NUMBER, TURBO-BLANKING- OFF

OFF= FILTER NUMBER, TURBO-BLANKING- OFFOFF= FILTER NUMBER, TURBO-BLANKING- OFF

INPUT = MANUAL MOVE ON COMMAND

INPUT = MANUAL MOVE ON COMMAND INPUT = MANUAL MOVE ON COMMAND

10. The Lambda DG-4 is under LOCAL (keypad) control upon start-up, as indicated by the words “INPUT - MANUAL” on the MAIN MENU (see above). MOVE ON COMMAND is the active operational mode at start-up, which means that filters are selected by pressing the numeric keys corresponding to the desired FILTER NUMBER (see EDIT A FILTER NUMBER definition of this value). The default FILTER NUMBER upon start-up is “0”. This setting will disrupt the optical pathway within the Lambda DG-4 and prevent any light output at the light guide port. The “0” FILTER NUMBER serves, then, as a “shutter” whenever it is selected.

11. Press the “1” key followed by ENTER. This FILTER NUMBER is pre-programmed at the factory to cause the light to be directed through the FILTER HOLDER that is loaded into position 1 and then onto the light guide with maximal light output (i.e., minimal attenuation).

12. Now experiment by selecting FILTER NUMBERS 0 through 4. Anytime you select a single digit FILTER NUMBER you must complete the entry by pressing the ENTER key or you must precede the single digit with a “0”. FILTER NUMBERS 0,1,2,3 and 4 are pre-programmed to correspond with the closed “shutter” and the four FILTER POSITIONS (with minimal attenuation), respectively.

Now that you have completed the “Make it Glow” section of the manual, you should be familiar with the basic components and operation of the Lambda DG-4. To get set up for an experimental application you will need to:



Install the LIGHT GUIDE into the OPTICAL HOLDER (if not already in place).



Install the OPTICAL HOLDER into the microscope using the appropriate adapter (if not already in place).



Install the desired filters into the FILTER HOLDERS (if not already in place).



Program the desired FILTER NUMBERS.



Establish the desired interface between the Lambda DG-4 and your computer.

Each of these steps is discussed in the following sections of the manual.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

4. O PTIC AL P ATH W AY

OPT ICAL PA T HWA Y

4.4.

OPT ICAL PA T HWA YOPT ICAL PA THWA Y

4.1

4.1 Light Guide & Optical Holder Installation

Light Guide & Optical Holder Installation

4.14.1

Light Guide & Optical Holder InstallationLight Guide & Optical Holder Installation

4.1.1

4.1.1 Liquid Light Guide

Liquid Light Guide

4.1.14.1.1

Liquid Light GuideLiquid Light Guide

The light output of the Lambda 10 DG4 is focused onto one end of a liquid light guide. The output of the liquid light guide can then easily be coupled to your microscope or optical system using correct mechanical adapter for your microscope. Please note that the light guide has a minimum-bending radius of 40 mm (about 1.6 inches).

4.1.2

4.1.2 Liquid Light Guide Installation

Liquid Light Guide Installation

4.1.24.1.2

Liquid Light Guide InstallationLiquid Light Guide Installation

The liquid light guide should have black sleeves over the ferrules on both ends. These ferrules, or “end sleeves”, are designed to slide into the mounting flange on the lambda DG-4 cabinet and the output adapter. Insert the end of the light guide into the light guide port until the top of the black end sleeve is even with the top of the light guide port. Tighten the locking screw on light guide port to secure the end of the light guide in position. This should result in maximal or near-maximal light output from the light guide. Minor adjustments up from this position may improve output; the adjustments can be easily made once the system is fully installed.

Figure 4-1. Liquid light guide installation.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

4.1.3

4.1.3 Optical Holder

Optical Holder

4.1.34.1.3

Optical Holder Optical Holder

The free end of the light guide slides into one end of the optical holder tube (shown below). The other end of the optical holder tube has a UV grade fused silica lens system (in the “short cup”). By sliding the light guide toward or away from these lenses, the light guide output can be collimated or brought to a focus. The optical holder, in turn, slides into the dovetail adapter, which has a “dovetail” collar on one end. This collar is used to fasten the entire assembly to the microscope system using an adapter (not shown) that is specific to the microscope make and model. Refer to the documentation included with each microscope adapter for further installation instructions.

Figure 4-2. Light guide optical holder.

4.2

4.2 Loading Filters

Loading Filters

4.24.2

Loading FiltersLoading Filters

4.2.1

4.2.1 Filter Orientation

Filter Orientation

4.2.14.2.1

Filter OrientationFilter Orientation

The two sides of a filter are usually not identical due to differences in their surface coatings. Some form of labeling will be present to differentiate the two sides. It is important to refer to the filter manufacturer’s documentation to interpret the labeling and determine the proper orientation relative to the light source. Remember, the light path inside the Lambda DG-4 travels from BOTTOM TO TOP. Therefore, the filters should be oriented with their “Lamp Side” down.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

4.2.2

4.2.2 Loading Filters

Loading Filters

4.2.24.2.2

Loading FiltersLoading Filters

At the end of each filter holder is a filter cup assembly (shown below). There are at least two items installed into the filter cup at the factory: a threaded retaining ring and a spacer ring (to carry the force of the retaining ring to the unthreaded portion at the bottom of the cup). In two of the filter cups, there will also be a blanking disc. Installation of filters into the filter holders requires care and patience to avoid damaging the threads of the filter cups and retaining rings as well as the filters.

Figure 4-3. Filter holder assembly.

The threaded retaining ring has two notches cut into it, 180 degrees apart. Place the notched end of the brass spanner wrench into the notches on the retaining ring and carefully unscrew it. If a retaining ring has been previously over-tightened, it is possible that the filter cup will begin to unscrew from the slider. If this occurs, STOP. Turn the spanner wrench around and, using the other end, insert it into the slots at the top of the cup. Then screw the cup back into the slider. DO NOT push on the cup. Just screw it in until it seats firmly. It should then be possible to remove the retaining ring.

Once the retaining ring is removed, the spacer (and blank, if present) should fall out. You should become familiar with the assembly of this retaining system. In particular, you should develop a feel for tightening the retaining ring without pushing on the wrench. This is important because if you forcefully push on the filter holder while you are trying to load a filter, you run the risk of scratching the filter.

Figure 4-4. Filter holder assembly with blanking disc.

Place the desired filter into the filter cup, first. With some 1” filters extra care must be taken to avoid getting the filters jammed at an angle inside the filter cup.

Next, install the spacer ring into the filter cup. Unless the filter thickness precludes the use of the spacer ring, always install the spacer to provide maximum separation between the filter surface and end of the brass spanner wrench that is used to turn the retaining ring.

Finally, install the threaded retaining ring.

CAUTION: DO NOT OVER TIGHTEN THE RETAINING RING!

CAUTION: DO NOT OVER TIGHTEN THE RETAINING RING!CAUTION: DO NOT OVER TIGHTEN THE RETAINING RING!

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

4.2.3

4.2.3 Selection of a Filter Position

Selection of a Filter Position

4.2.34.2.3

Selection of a Filter PositionSelection of a Filter Position

In most cases, any given filter could be installed at any of the four FILTER POSITIONS. However, some minor considerations may improve performance of the system. Adjacent FILTER POSITIONS will require slightly less switching time. Therefore, if an experiment will require rapid switching between two filters they should be located next to one another. If separated by one or more filters, there will be brief transitions through the intervening filter(s) unless the TURBO-BLANKING feature is enabled (see “Modes of Operation”). While this feature eliminates the transmission of light at unwanted wavelengths, it does further increase the time required for switching.

4.2.4

4.2.4 Blanking Discs

Blanking Discs

4.2.44.2.4

Blanking DiscsBlanking Discs

The blanking discs should only be removed from those positions that will contain a filter. The discs are intended as a safety device for the occasion where you may be looking into the microscope. We strongly urge that the discs be used. Please be careful and avoid the possibility of direct visualization of high intensity light.

4.2.5

4.2.5 Neutral Density Filters

Neutral Density Filters

4.2.54.2.5

Neutral Density FiltersNeutral Density Filters

. Filter installation is the same for this filter holder as described above. The slide portion of the neutral density filter holder, however, is mechanically different from those used for the interference filters. As a result, the two types of holders may not be interchanged.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

4.2.6

4.2.6 Filter Holder Installation

Filter Holder Installation

4.2.64.2.6

Filter Holder InstallationFilter Holder Installation

Identify the access doors used to install the neutral density FILTER HOLDER and the four interference FILTER HOLDERS. The small access door located near the top edge of the left side panel on the Lambda DG-4 cabinet (see below) is for the neutral-density FILTER HOLDER. The larger door in the middle of the left side panel is fro the interference FILTER HOLDER. Loosen the black knob on these access doors to slide them up and install the appropriate FILTER HOLDER into the slot. The bottom of the neutral density FILTER HOLDER is flat while the bottoms of the interference FILTER HOLDERS are grooved. For this reason, these two types of FILTER HOLDERS are not interchangeable. Make certain that the end nearest the filter cup goes in first.

Figure 4-5. Lambda DG-4 left side.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

5. C O NTR OLL E R

CON TROL LER

5.5.

CON TROL LERCON TROL LER

Although the Lambda DG-4 is simple to use, proper operation depends on the user understanding the design and characteristics of this instrument. This section provides the detailed information needed to begin operation and to control the unit from its keypad.

5.1

5.1 Control Electronics

Control Electronics

5.15.1

Control ElectronicsControl Electronics

The control electronics for the filter changer are activated by the “MAIN” power switch on the rear of the unit. You may operate the control electronics without the lamp on, but it is best to turn the control electronics off before the lamp is started. Once the lamp has been started, the control electronics may be turned on again.

5.2

5.2 Power

Power----up Sequence

5.25.2

PowerPower

There are two switches on the back of the Lambda DG-4 labeled LAMP and MAIN. The LAMP switch will ignite and power the xenon lamp. The MAIN switch applies power to the controller and the fan that cools all of the electronics (including the xenon lamp power supply). The MAIN switch should be turned on immediately after the LAMP switch is turned on. If you are only configuring the Lambda DG-4 controller or a computer interface, you do not need to switch on the LAMP before turning on the MAIN switch. The following message will be briefly displayed on the front panel when the MAIN switch is turned on:

After a few seconds the MAIN MENU will be displayed:

At this point, the Controller subsystem is ready for operation.

up Sequence

up Sequenceup Sequence

SUTTER FAST FILTER CONTROLLER PROGRAM

SUTTER FAST FILTER CONTROLLER PROGRAM SUTTER FAST FILTER CONTROLLER PROGRAM

OFF= FILTER NUMBER, TURBO-BLANKING- OFF

OFF= FILTER NUMBER, TURBO-BLANKING- OFFOFF= FILTER NUMBER, TURBO-BLANKING- OFF

INPUT = MANUAL MOVE ON COMMAND

INPUT = MANUAL MOVE ON COMMAND INPUT = MANUAL MOVE ON COMMAND

When the unit is first turned on, the FILTER NUMBER indicates that the filter selecting mirrors have been positioned to prevent lamp output from the device. The status of the TURBO OFF is the default setting for that feature when the controller is first powered up.

The second line of the display indicates if the unit is under local control and that the unit will execute all commands as they are received.

The unit selects the PARALLEL MODE as the default remote interface at power on. If the unit is under remote control at power on, the appropriate remote interface mode will be automatically set and the display may change immediately to reflect any waiting computer commands.

LAMBDA DG-4 & DG-5 OPERATION MANUAL – REV. 2.14 (20081107)

TURBO----BLANKING

TURBOTURBO

FILTER NUMBER display will read “OFF”. This

FILTER NUMBERFILTER NUMBER

BLANKING feature (discussed below) is also shown;

BLANKINGBLANKING

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Sutter Instrument DG-4, DG-5 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual