Stuart 8500 Instruction Manual

Microcentrifuge 8500

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Operator's Manual

RoHS

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Product Description

Thank you for your purchase of the Stuart Microcentrifuge. This centrifuge has been designed for
applications requiring relatively low g-force, such as microfiltration, separation or quick spin downs
of samples. The product is supplied with an 8-Place rotor for 0.5 – 2.0 mL tubes, a 4-Place PCR
rotor that can hold 8 PCR strips and 8, 0.5, tube adapters.

Package Contents

• Stuart Microcentrifuge: 1 Unit

Cole-Parmer Cat. No.: 17414-70
Stuart Cat. No.: SCF4

• 8-Place Rotor for 0.5 - 2.0mL Tubes: 1 Each

Cole-Parmer Cat. No.: 17414-71
Stuart Cat. No.: SCF4/1

• 4-Place, 8 Strip PCR Rotor: 1 Each

Cole-Parmer Cat. No.: 17414-72
Stuart Cat. No.: SCF4/2

• 0.5mL Tube Adapters (8 each): 1 Pack

Cole-Parmer Cat. No.: 17414-73
Stuart Cat. No.: SCF4/V05

• Power Cord: 1 US, 1 EU, 1 UK

• Operator's Manual: 1 each

Specifications

Catalog Numbers 17414-70, SCF4
Maximum speed 8500 rpm
Maximum G-Force (RCF) 3884

Capacity

Electrical 100-240 VAC, 50/60 Hz, 1 A Maximum
Dimensions 7.8 x 7.3 x 4.3 in / 198 x 185 x 109 mm
Weight 2.75 lbs / 1.25 kg

Fuse

Noise level (without tubes) <= 60 Db measured at 1 meter from centrifuge

8-place rotor for 0.5 – 2.0 mL tubes;4-place, 8-strip PCR rotor
for 0.2 mL tubes

Type: 5x20 mm SLO-BLOW 0.6 A /250 V for 200-265 VAC
Operation 1.0 A/250 V for 85-160 VAC Operation

Operating Conditions

Ambient conditions Indoor use only, in well ventilated area
Temperature range 5°C to 40°C
Relative Humidity 10% to 80% (no condensation)
Altitude 0 to 2,000 meters
Power supply Fluctuation not to exceed 10%
Power supply overvoltage Category II
Environment rating Pollution Degree 2

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Maintenance, Care and Safety Precautions

Important Safety Information

Please read the following safety precautions and instruction manual before
operating the Stuart Microcentrifuge.

• NEVER use the Microcentrifuge in a manner not specifi ed in these instructions.

• NEVER use solvents or fl ammable liquids in or near this equipment.

• NEVER operate in a hazardous or fl ammable environment.

• NEVER use solvents on the unit that may attack plastic or cause cracks in the rotor.

• NEVER centrifuge materials that in combination or alone are fl ammable, explosive, are
corrosive or that may cause any other hazard or damage this equipment.

• NEVER use materials with a density greater than 1.2g/cm, as this unit is not intended to
centrifuge very dense materials.

• NEVER immerse the Microcentrifuge in water.

• NEVER move the Microcentrifuge while the unit is in operation.

• NEVER operate the Microcentrifuge with the lid open or removed.

• NEVER fi ll tubes while they are in the rotor; liquid spills will cause damage to the Microcentrifuge.

• NEVER operate the Microcentrifuge unless the rotor is fi rmly attached, the tubes are securely
closed and the rotor is balanced.

• NEVER use damaged or cracked rotors.

• NEVER insert non standard tubes or other foreign articles into the rotor.

• NEVER put hand or any object in the rotor area unless the rotor has completely stopped.

• Always only use microcentrifuge tubes and tube strips made from plastic designed to
withstand centrifuged forces of at least 3884 g's (RCF).

• Always only use original rotors, power cord, tube adapters and any other spare parts and
accessories supplied by the centrifuge manufacturer.

• Always remove the power cord from the outlet prior to maintaining or cleaning the
Microcentrifuge.

• Always observe general laboratory safety precautions and regulations when using this product.

• Always use the proper cleaning agents when performing maintenance on the Microcentrifuge.

• Always clean the rotor with a pH-neutral cleaning liquid to ensure the rotor, housing, lid and lid
latches are protected from corrosion and/or mechanical damage.

• Always follow all safety measures from the Safety Data Sheet (SDS) for any reagent you use
with/within the Microcentrifuge.

• Before using any cleaning or decontamination methods, except those recommended in
this manual, please contact Cole-Parmer to ensure that the proposed method will not damage
the equipment.

• If a single level of hazard protection is required, use containers that are tested to Risk Group II or
above and, that more than one level of protection is provided for materials of a higher Risk Group.

• If a hazardous material is spilled in and/or on the product, the user has the responsibility for
carrying out appropriate decontamination.

• The Microcentrifuge is intended to be used indoors (only).

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Maintenance, Care and Safety Precautions

• Store the Microcentrifuge in a dry area at room temperature. Do not expose to moisture,
sunlight, or excessive temperatures for extended periods of time.

• The Microcentrifuge is designed for separating aqueous solutions in specifi c tubes
that are compatible with the G-Force (RCF) of this particular unit.

• Always work and handle this product and/or materials in a manner that DOES NOT endanger
the user, any other person(s) or the environment.

WARNING! This product does not contain bio-seals as per IEC/EN/CSA

61010-2-20 nor does it provide any level of containment in the event of a release

and/or spill of radioactive, toxic, or pathogenic micro-organisms; therefore

these types of materials are not recommended for use in this product.

WARNING! Reference Safety Data Sheet (SDS), wear essential

protective equipment and adhere to all applicable local and national

regulations before dispensing and/or disposing of hazardous substances.

WARNING! Your laboratory protocol must state that no person(s)

and/or hazardous substance(s) should be within 12" (30 cm)

WARNING RISK OF EARTH
ELECTRIC SHOCK

of this centrifuge during operation.

SYMBOLS DEFINED

Electrical Requirements

THIS INSTRUMENT MUST BE GROUNDED

Before connection please ensure that the line supply corresponds to
the power requirements below:

The unit is provided with a power adaptor and three power cables: a UK 3-pin and a “EU” 2-pin
plug for 230 V installations and a US plug for 120 V installations.

Choose power cable appropriate for your electrical installation and discard the others. Should none of
the cables be suitable for connecting to the power supply, replace the plug with a suitable alternative.

OPERATION SHOULD ONLY BE UNDERTAKEN BY A QUALIFIED ELECTRICIAN.

NOTE: Refer to the equipment rating plate to ensure that the plug and fusing are suitable

230V 120V

BROWN - HOT/LIVE BLACK - HOT/LIVE
BLUE - NEUTRAL WHITE - NEUTRAL
GREEN/YELLOW – EARTH GREEN - EARTH

The appropriate power cable should be connected to the instrument BEFORE connection to
the electrical outlet. Should the power cable require replacement, a cable of .75 mm² (AWG18) of
harmonized code H05VV-F connected to an IEC320-C13 plug should be used.

Power: 36W Supply requirements: 100 - 240 VAC, 50/60Hz

for the voltage and wattage stated. The wires in the power cable are as follows:

IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN

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Operating Instructions

1. Remove the Microcentrifuge from the box and examine all of the contents for breakage or miss­ing parts. Save all packaging and paperwork.

2. Place the Microcentrifuge on a fl at, stable surface. The unit should be placed in a
non-hazardous environment and located near an electrical outlet.

3. With the power switch in the off position, attach the power cord to the back of the
Microcentrifuge and to the grounded electrical outlet.

4. Turn the power switch to the "ON" position. The Microcentrifuge will automatically perform a
safety check on the locking mechanism. Open the lid by pressing the illuminated button located
on the front of the unit.

5. Insert your pre-loaded sample tubes into the rotor. Make sure all tubes are fi lled to equal levels
and the lids are closed. Tubes must be balanced properly.

6. Close the lid and the Microcentrifuge will begin the centrifugation cycle. IMPORTANT: If
there are loud / unusual sounds or excessive vibrations discontinue operation immediately.
See the Troubleshooting section in this manual. If you cannot correct the problem, contact
Cole-Parmer for assistance. DO NOT CONTINUE TO OPERATE!

7. When centrifugation is completed, once again, press the illuminated button on the front of unit to
stop the spin cycle motor and open the lid. Do not open lid until rotor comes to a complete stop!
Remove your samples from the rotor.

8. The Microcentrifuge lid is secured by two locking pins that prevents accidental lid opening during
operation and greater safety for the operator. When the Centrifuge has completed the spin cycle,
an automatic lid unlock will occur. If the lid does not open, press and hold the button on the front
of the unit for one full second, the Centrifuge will unlock the lid again. Should the lid fail to open
by pressing the front button or in the event that the power to the Microcentrifuge is interrupted,
the Microcentrifuge lid can also be opened manually by inserting a fl at head screw driver into the
bottom of the unit and moving the release lever to the right. This will release the pins and allow
the lid to open. See Figure 1, page 6.

9. The Microcentrifuge has been designed with an automatic shut-off feature that discontinues
operation after 60 minutes of continuous use. This will prevent the unit from running
continuously if left unattended.

CAUTION: Spin Balanced Loads Only

For proper operation, samples must always be loaded into the rotors in a balanced fashion. The
tubes should be filled to the same level, lids closed and placed opposite each other in the rotor.
Tubes and strips must always be loaded symmetrically.

CAUTION: Do Not Open Lid Until

Rotor has Completely Stopped

Rotor Installation and Replacement

The Microcentrifuge features snap on rotors that can be installed and removed without an Allen
wrench. There are no set screws to tighten or loosen.

1. Make sure the Microcentrifuge's On/Off switch is in the "Off" position.

2. Line triangular opening on the rotor with the rotor shaft and press down until the rotor snaps in

place. Pull up on the rotor to remove.

3. Turn the unit back to the "On" position, load tubes per the operating instructions and close the lid

to begin centrifugation.

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Maintenance & Cleaning

CAUTION: Disconnect the Microcentrifuge

from the power outlet before cleaning. Never

pour liquid into the unit or submerge in water

The Microcentrifuge is maintenance free other than occasional cleaning. There are
no user serviceable parts inside the housing other than replacing the fuse.

• To replace the fuse, make sure the On/Off switch is in
the "Off'' position, place the unit on its side and insert
a fl at head screw driver into the fuse holder found
on the base and turn counter-clockwise until the
fuse is released. Replace the fuse. Reposition
the fuse holder and turn clockwise until fuse
holder is secure, be careful not to over-tighten.
Return the switch to the "On" position and
begin operation.

• To clean, remove the rotors and clean with
isopropyl alcohol. The housing and lid can be
cleaned with a damp cloth and a mild
non-corrosive detergent.

• After cleaning, ensure all parts are dried thoroughly
before attempting to operate the unit.

Figure 1

Troubleshooting

Error Cause Solution

Unit does not operate

Loose cord.
On/Off switch.
Lid not closed.

Blown fuse. Replace fuse.

Check cord.
Turn switch to "On" position.
Close lid until it clicks and locks in

place.

Fuse

Manual lid

release

Rotor not balanced. Check tube fl uid levels and symmetry.

Excessive noise or
vibration

Lid will not open when
button is pressed

Unit stops running
without pressing front
button

Rotor not installed
properly.

Foreign object in unit.

Power is off.

Loose cord. Check cord.
Lid not completely closed. Close lid until both pins are locked.

60-minute time limit
expired.

Remove & reinstall rotor per above
instructions.

Check centrifuge bowl for loose tubes
or objects.

See above.
Open lid manually.

Centrifugation cycle is complete.

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Warranty Information

Microcentrifuge 2 Year Warranty
Microcentrifuge is warranted to be free of defects in materials and workmanship for 24 months

from the date of purchase, providing that:

• The unit has not been opened or attempted to be opened by the end user.

• The user ensures that it has been carefully cleaned and decontaminated before returning.

• The unit has been operated according to the instructions and not in any improper way.

• The unit has not been damaged due to negligence, accident or used under abnormal conditions
(temperature, corrosives, unbalanced rotors, lack of cleaning, liquid introduced into housing).

• Normal wear and tear of unit.

In order to qualify for the warranty repair; the customer must submit all warranty information
required to validate proof of purchase. Visit ColeParmer.com/product-warranty-registration
to submit information.

Should warranty service be required, repair or replacement will be provided by Cole-Parmer without
charge. Freight to ship the unit to Cole-Parmer will be the responsibility of the purchaser. There are
no other warranties or guarantees, implied or otherwise expressed including any warranties of
application or merchantability.

In no event will this warranty obligation exceed price of product. This includes liability claims of loss
of use, lost profi ts or any exemplary or punitive damages.

Repair

Should your Stuart Microcentrifuge require service or repair, please contact Cole-Parmer customer
service. They will issue a return authorization number and advise on the proper packaging and
method of shipment.

Cole-Parmer USA
1-800-323-4340
1-847-549-7600

Please include your return authorization number on the shipping label.

Cole-Parmer UK
[phone]

This equipment is labeled with the crossed out wheeled bin symbol to indicate that the
equipment must not be disposed of with unsorted waste. It is your responsibility to
correctly dispose of the equipment by handing it over to an authorized facility for
separate collection and recycling. It is also your responsibility to decontaminate
the equipment in case of biological, chemical or radioactive contamination. so as
to protect from health hazards the persons involved in the disposal or recycling of
products.

For more information about where you can drop off your waste equipment, please
contact your local dealer from whom you originally purchased the product.

By doing so, you will help to conserve our natural resources and you will ensure
that your waste products are recycled in a matter that protects human health and
protects the environment.

Thank you.

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This product meets the applicable harmonized standards for radio frequency interference
and may be expected not to interfere with, or be affected by, other equipment with similar
qualifi cations. We cannot be sure that other equipment used in its vicinity will meet these

standards and so we cannot guarantee that interference will not occur in practice. Where
there is a possibility that injury, damage or loss might occur if equipment malfunctions due to radio
frequency interference, or for general advice before use, contact the manufacturer.

EU Declaration of Conformity

Product Laboratory Equipment

Manufacturer Cole-Parmer

625 East Bunker Court
Vernon Hills
IL 60061
USA

This declaration of conformity is issued under the sole responsibility of the manufacturer

Object of Declaration

The object of the declaration described above is in conformity with the relevant Union Harmonisation Legislation:

References to the relevant harmonised standards used or referen
to which conformity is declared:

IEC/EN 61010-1:2010

Low Voltage Directive
EMC Directive
RoHS Directive 2011/65/EC

Microcentrifuge 8500, SCF4

(reference the attached list of catalogue numbers)

2014/35/EU
2014/30/EU

Safety requirements for electrical equipment for
measurement, control and laboratory use.
Part 1: General requirements.

Cole-Parmer

625 E Bunker Court
Vernon Hills, IL 60061
Toll-free: 1-800-323-4340
Phone: [phone]
Fax: [phone]
ColeParmer.com

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IEC/EN 61010-2-020:2016

IEC/EN 61326-1:2013

Signed for and on behalf of the above manufacturer

Additional Information

Place of Issue

Date of Issue

Authorised Representative

Title

Signature

Particular requirements for laboratory centrifuges.

Electrical equipment for measurement, control and
laboratory use. EMC requirements.
Part 1: General requirements (Class A).

Year of CE Marking: 2018

Vernon Hills, IL, USA

December 20, 2018

Pete Hadjis

CPVH Product Development Manager

s.

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