STRATAGENE DNA Markers User Manual
DNA Markers
NUCLEIC
ACID &
ANALYSIS
DNA Markers
Our DNA marker portfolio offers a variety of
markers featuring a wide range of fragment
+ High ladder purity — ensures sharp bands for accurate sizing
+ Known band masses — for simple relative yield determination
+ Specific bright intensity bands — allow for quick reference and
orientation
+ Combined ladders — maximize size range
+ Easily radio-labeled — perfect for Northern or Southern blot
analysis
sizes with known mass amounts to help you
make accurate size determination and
approximate the mass of similarly sized DNA
fragments.
Our collection of DNA markers serves as ideal gel electrophoresis
standards from 8 bp to 23 kb. With known mass of DNA in each of
the bands and the ability to use any DNA intercalating dye such as
ethidium bromide for visualization, our ladders are easy to use for
relative mass estimation of fragments of similar size. We also offer
combined digests to maximize the ladder range for straight forward
and accurate sizing. Additionally, our Kb DNA Ladder and
Supercoiled DNA Ladder include specifically bright bands as a quick,
clear visual reference point. All ladders are double stranded DNA
and provided in 10 mM Tris-HCL (pH 7.5), 1 mM EDTA solution.
DNA Marker Porfolio
Kb DNA Ladder
Supercoiled DNA Ladder
Lamda/
Hin
d III Marker
ΦX174/
Hae
III Marker
Lambda/
Hin
d III- ΦX174/
ΦX174/
Hin
f I Marker
Illuminator™Kb DNA Ladder
Illuminator™Nonradioactive Lambda/
Hae
III Marker
Hin
d III Marker
NUCLEIC
ACID &
ANALYSIS
DNA Markers
—12 kb
—10 kb
—8 kb
—6 kb
—5 kb
—4 kb
—3 kb
—2 kb
—1.5 kb
—1 kb
—750 bp
—500 bp
———— 9 kb
———— 7 kb
—250 bp
— 12 kb
— 10 kb
—9 kb
—8 kb
—7 kb
—6 kb
—5 kb
—4 kb
—3 kb
—2 kb
— 23.130— 23,130 bp
— 9,416 bp
— 6,557 bp
— 4,361 bp
— 2,322 bp
— 2,027 bp
—564bp
— 125 bp
— 1,353 bp
— 1,078 bp
—872bp
— 603 bp
— 310 bp
— 281, 271 bp
— 234 bp
— 194 bp
—118bp
— 72 bp
—2,322 bp
—2,027 bp
—1,353 bp
—1,078 bp
—872 bp
—603 bp
—564 bp
—310 bp
—281, and 271 bp
—234 bp
—194 bp
—125 bp
—118 bp
—72 bp
—23,130 bp
—9,416 bp
—6,557 bp
—4,361 bp
726 bp—
713 bp—
553 bp—
500 bp—
427,417, and 413 bp—
311 bp—
249 bp—
200 bp—
151 bp—
140 bp
—
118 bp—
100 bp—
82 bp—
66 bp—
48, 42, and 40 bp—
24 bp—
— 12,000 bp
— 500 bp
—750bp
— 1,000 bp
—1,500 bp
— 2,000 bp
— 3,000 bp
— 4,000 bp
— 5,000 bp
— 6,000 bp
—23,130 bp
—9,416 bp
—6,557 bp
—4,361 bp
—2,322 bp
—2,027 bp
—565 bp
—125 bp
Kb DNA Ladder
Supercoiled
DNA Ladder
Lambda/
Marker
Hin
d III
φX174/
Marker
Hae
Hin
f I
III
Lambda/
Hin
d III-
φX174/
Hae
III
Marker
φX174/
Marker
™
Illuminator
Need More Information? Give Us A Call:
Stratagene US and Canada
Order: 800-424-5444 x3
Technical Service: 800-894-1304 x2
Stratagene Europe
Order: 00800-7000-7000
Technical Service: 00800-7400-7400
For a list of worldwide distributors, please visit our website.
Kb
DNA Ladder
Stratagene Japan K.K.
Order: 3-5821-8077
Technical Service: 3-5821-8076
www.stratagene.com
Illuminator
Nonradioactive
Lambda/
Hin
Marker
Product Description Amount Catalog No.
Kb DNA Ladder 15 fragments: 250 bp to 12 kb 250 μg 201115
Supercoiled DNA Ladder 10 fragments: 2 kb to 12 kb 500 μg 201116
Lambda/
Hin
ΦX174/
Hae
™
d III
Lambda/
ΦX174/
Hind
Hin
f I Marker
Illuminator™Kb DNA Ladder
Illuminator™Nonradioactive
Lambda/
Hin
Illuminator™is a trademark of Stratagene in the United States.
d III Marker 8 fragments: 125 bp to 23 kb 500 μg 201109
1000 μg 201104
III Marker
11 fragments: 72 bp to 135 bp 20 μg 201100
100 μg 201101
III- ΦX174/
Hae
III Marker
27 fragments: 8 bp to 23 kb 100 μg 201107
21 fragments: 24 to 276 bp 20 μg 201102
100 μg 201103
15 fragments: 250 bp to 12 kb 1.5 μg 201117
d III Marker
8 fragments: 125 bp to 23 kb 1.5 μg 201130
© Stratagene, an Agilent Technologies company, 2008
Printed in USA, April 2008
FL 23 (71204) 5989-8243EN
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