Steri-Cult 3307 Series, 3310 Series Operating And Maintenance Manual

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Steri-Cult

Model 3307 and 3310 Series CO2 Incubator Controlled RH with Sterilization Cycle

Operating and Maintenance Manual 7023307 Rev. 15

Thermo Scientific

MANUAL NUMBER 7023307

15 28727 6/18/12 Clarified nebulizer flush instructions - pg 7-4 & 7-5 ccs

14 28408 5/18/12 Chg’d 95% non-condensing in Specifications to 90% ccs

13 27891 10/27/11 Clarified Water Empty alarm language on pg 5-3 ccs

12 27292/IN-4073 9/28/11 400119 to 400201 switcher kit - pg 11-1 ccs

11 27162/IN-4071 3/24/11 Added pilasters and shelf channels to Parts Listccs

-- -- 11/24/10 Corrected typo in chart above: 3308/3310, medium/large ccs

10 25128/IN-3882 4/7/10 Missed correction from water bottle change (pgs 1-10, 7-3 & 7-4) ccs

9 25480/SI-10033 11/4/09 Common shelf channel (pg 1-7 & 1-8 artwork and 11-1 parts list) ccs

8 25128/IN-3882 9/21/09 Updated water bottle to current production (0.5 gal) ccs

7 25387/SI-10033 4/2/09 Removed soft-stops from page 1-8 ccs

Preface

7023307 Steri-Cult i

Model Size Voltage

3307 Medium 115

3308 Medium 230

3310 Large 115

3311 Large 230

Preface

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Caution If the incubator is not used in the manner specified in this operating manual, the protection provided

by the equipment design may be impaired. s

Material in this manual is for information purposes only. The contents and the product it describes are subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of this manual.

Thermo Scientificii 7023307 Steri-Cult

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher Scientific has contracted with one or more recycling/disposal companies in each EU Member State European Country, and this product should be disposed of or recycled through them. Further information on Thermo’s compliance with this directive, the recyclers in your country and information on Thermo Scientific products will be available at www.thermofisher.com.

4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientific

7023307 Steri-Cult iii

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct 1-800-438-4851 Toll Free, U.S. and Canada 1-877-213-8051 FAX http://www.thermoscientific.com Internet Worldwide Web Home Page service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Page

Thermo Fisher Scientific 401 Millcreek Road, Box 649 Marietta, OH 45750

Our staff can provide information on pricing and give you quotations. We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you. Our products are listed on the Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation or troubleshooting of your equipment. We can fill your needs for spare or replacement parts or provide you with on-site service. We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service call.

When more extensive service is necessary, we will assist you with direct factory trained technicians or a qualified service organization for on-the-spot repair. If your service need is covered by the warranty, we will arrange for the unit to be repaired at our expense and to your satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by telephone or fax. If you wish to write, our mailing address is:

International customers, please contact your local Thermo Scientific distributor.

Sales Support

Service Support

www.unitylabservices.com

Thermo Scientificiv 7023307 Steri-Cult

Warranty Notes

Information You Should Know Before Requesting Warranty Service

• Locate the model and serial numbers. A serial tag is located on the unit itself.

• For equipment service or maintenance, or with technical or special application inquiries, contact Technical Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local distributor.

Repairs NOT Covered Under Warranty

• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for

temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as a billable service at your location. Calibration after a warranty repair is covered under the warranty.

• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to equipment materials.

• Service calls for improper installation or operating instructions. Corrections to any of the following

are billable services:

1) electrical service connection

2) tubing connections

3) gas regulators

4) gas tanks

5) unit leveling

6) room ventilation

7) adverse ambient temperature fluctuations

8) any repair external to the unit

• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper installation.

• Repairs to parts or systems resulting from unauthorized unit modifications.

• Any labor costs other than that specified during the parts and labor warranty period, which may include additional warranty on CO

2 sensors, blower motors, water jackets, etc.

Preface

Thermo Scientific

7023307 Steri-Cult v

Table of Contents

Section 1

Section 2

Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Inventory Management System . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Control Panels Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2

Operation of the Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

Stacking the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7

Installing the Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7

Installing the Access Port Filter Assembly . . . . . . . . . . . . . . . . . . . .1-8

Installing the HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9

Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10

Connecting the Unit to Electrical Power . . . . . . . . . . . . . . . . . . . .1-10

Filling the Humidity Water Bottle . . . . . . . . . . . . . . . . . . . . . . . .1-10

Connecting the CO

2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . .1-11

Humidity and Loading the Chamber . . . . . . . . . . . . . . . . . . . . . .1-12

Incubator Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Setting the Temperature Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Setting the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Setting the CO

2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3

Setting the Relative Humidity (RH) Setpoint . . . . . . . . . . . . . . . . . .2-3

Section 3

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Calibrating the Infrared CO

2 System . . . . . . . . . . . . . . . . . . . . . . . . .3-2

Calibrating Relative Humidity (RH) . . . . . . . . . . . . . . . . . . . . . . . . .3-3

7023307 Steri-Cult viThermo Scientific

Table of Contents

Section 4

Section 5

Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Turning the Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . .4-1

New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Setting the REPLACE HEPA Filter Reminder . . . . . . . . . . . . . . . . . .4-2

Setting an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Setting a Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

Enabling Temp Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . .4-3

Setting a Low CO Setting a High CO

2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Enabling CO2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .4-5

Setting a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5

Setting a High RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-6

Enabling RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .4-6

Selecting a Primary Tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7

Disabling the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7

Setting a RS485 Communications Address . . . . . . . . . . . . . . . . . . . .4-8

Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Temp Controller Failure TEMP CNTRL ERROR . . . . . . . . . . . . . .5-2

RH System Fault . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Gas Guard Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Water Level Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Door Open Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Section 6

Section 7

Section 8

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Cleaning the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Cleaning the Humidity System . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2

Cleaning the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2

Flushing the Humidity System . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-4

HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-

Sterilization Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

vii 7023307 Steri-Cult Thermo Scientific

Table of Contents

Section 9

Section 10

Section 11

Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

RS485 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-2

Connecting the Analog Output Boards . . . . . . . . . . . . . . . . . . . . . . .9-3

2 Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-5

Operation of the CO2 Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . .9-5

De-activating the Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-5

Connecting the CO

2 Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . .9-5

Dataloggger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-6

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1

Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1

Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-3

Replacing the CO

2 and RH Air Filters . . . . . . . . . . . . . . . . . . . . . . .11-3

Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-5

Wiring Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-8

Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-10

7023307 Steri-Cult viiiThermo Scientific

Section 1 Installation and Start-Up

Figure 1-1. Steri-Cult Components

The incubator’s major components are described below.

• Chamber Gas Sample Port - Used for sampling chamber CO

2 content

using a FYRITE or similar instrument.

• Power On/Off Switch - Mains disconnect

• Control Panel - Keypad, Displays & Indicators (Figure 1-3)

• Fold-down Storage Area - Store manuals, markers, etc.

• Leveling Feet - Used to level the unit

Note The incubators are stackable. Information follows in this section.

7023307 Steri-Cult 1-1Thermo Scientific

Section 1

Installation and Start-Up

Inventory

Management System

Included in the parts bag shipped with each unit is an inventory management system. This system consists of:

• A steel plate

• Five different colored magnets

• Five heat-resistant shelf labels

• Dry-Erase marker

Hang the plate on the inside of the outer door, using the keyholes and hardware already installed on the door. Attach a colored shelf label to the front edge of each shelf. The magnets may be aligned on the plate, in order according to shelf label color. Use the marker to record information about the product on corresponding shelves. The magnets can be erased as needed and rewritten.

Caution The inventory management system magnet plate will not

withstand the heat of the sterilization cycle. Simply unhook the whole plate from the outer door and set aside before initiating the cycle, then reinstall afterward.

Figure 1-2. Inventory System

Control Panels Keys,

Displays & Indicators

Figure 1-3. Control Panel Components

1-2 7023307 Steri-Cult Thermo Scientific

Section 1

Installation and Start-Up

Control Panel

(continued)

Silence - Silences the audible alarm.

Alarm indicator - Light pulses on/off during an alarm condition in the

cabinet.

Mode select switch - Used to select Run, Setpoints, Calibration and

System Configuration Modes.

Message center - Displays system status.

Mode select indicators -

Run: Run Menu

Settings: Set Points Menu

Calibrate: Calibration Menu

Configuration: Configuration Menu

Up / down arrows - Increments number values, toggles between choices.

Enter - Stores the value into computer memory.

Heat indicator - Lights when power is applied to the heaters.

Heat display - Displays temperature (°C)

Humidity indicator - Lights when humidity is required

RH display - Displays humidity (%)

Operation of the

Keypad

Left and right arrows - Moves the operator through the choices of the

selected mode.

Inject indicator - Lights when CO

chamber.

2 display - Displays CO2 (%)

Sterilization button - Starts Sterilization Cycle

The Steri-Cult incubator has four basic modes which allow incubator setup: Run, Settings, Calibrate and System Configuration.

Run is the default mode which the incubator will normally be in during

operation.

Settings is used to enter system setpoints for incubator operation.

Calibrate is used to calibrate various system parameters.

Configuration allows for custom setup of various options.

2 is being injected into the incubator

7023307 Steri-Cult 1-3Thermo Scientific

Section 1

Installation and Start-Up

Keypad (continued)

The chart below shows the selections under each of the Modes. Base Unit Displays are in bold type and Option Displays are in italics.

Run Settings Calibrate Configure Default Temperature Temp Cal Audible

Overtemp CO2 Cal New HEPA Timer CO2 RH Cal Replace HEPA Reminder RH Access Code

Temp Lo Alarm Temp Relay CO2 Lo Alarm CO2 Hi Alarm CO2 Relay RH Lo Alarm RH Hi Alarm RH Relay

Tank Select Gas Guard

RS485 Address

Right and Left Arrows: Steps the operator through the parameters of Settings, Calibrate and Configuration Modes. The right arrow goes to the next parameter, the left arrow returns to the previous parameter.

Up Arrow: Increases or toggles the parameter value that has been selected in the Settings, Calibrate, and Configuration Modes.

Enter: Must press Enter key to save to memory all changed values.

Down Arrow: Decreases or toggles the parameter values that have been

selected in the Settings, Calibrate and Configuration Modes.

Silence Key: Press to silence the audible alarm. See Section 5 for alarm ringback times.

1-4 7023307 Steri-Cult Thermo Scientific

Section 1

Installation and Start-Up

Displays

Installing the

Incubator

Message Center: Displays the system status (Mode) at all times. Displays CLASS 100, SYSTEM OK or RH WAITS ON TEMP (see Setting the RH Setpoint for further details) during normal operation. Alarm messages display if the system detects an alarm condition. See Section 5, Alarms. The display message CLASS 100 is a timing mechanism indicating that, under normal operating conditions with the HEPA filter installed, the air inside the chamber meets the Class 100 air cleanliness standard for particulates of 0.5 micron size or larger per cubic foot of air. (For further information on the Class 100 classification of air quality, see Appendix A.)

3 Upper Displays: The first upper display shows the temperature. The second display shows the percentage of CO

2. The third display shows the

humidity percentage.

Caution Single and stacked units must be installed against a wall or similar

structure. s

1. Maintain a three-inch clearance behind the incubator for electrical and gas hook-ups. In addition, a three-inch ventilation space is needed on each side of the unit.

2. Locate the unit on a firm level surface capable of supporting the unit’s weight (340 lbs.-Models 3307 & 3308, 410 lbs.-Models 3310 &

3311).

3. Locate the unit away from doors and windows and heating and air conditioning ducts.

Caution Lift the unit only by the sides of the cabinet base. Do not attempt

to lift by the front and back. This places stress on the outer door hinges. s

7023307 Steri-Cult 1-5Thermo Scientific

Section 1

Installation and Start-Up

Stacking the Incubators

Warning With incubators in a stacked configuration, do not leave both

exterior doors open at the same time.

Warning If the units have been in operation, turn them both off and

disconnect the power before beginning any service work. s

Four stacking brackets (one shown at right) are included in the parts bag shipped with each incubator.

1. Unscrew and remove the 4 leveling feet from the unit to be stacked on top and lift it onto the base unit. Align all sides.

Warning This incubator weighs 340 lbs. for Models 3307 & 3308, 410 lbs.

Figure 1-4.

Stacking Bracket

for Models 3310 & 3311. Have sufficient personnel available when lifting. Lift the unit by the sides of the cabinet base to avoid placing stress on the outer door hinge.

2. Remove the hole plugs from the stacking bracket holes (Figure 1-6).

3. Align the holes in the brackets with the mounting holes on the sides of the top and bottom incubator. Secure the two brackets with the stainless steel screws and washers provided in the parts bag. See Figure 1-5.

4. The stacked incubators are ready for service.

Figure 1-5. Stacking Brackets Installed

1-6 7023307 Steri-Cult Thermo Scientific

Figure 1-6. Sides and Back of Stacked Units

Section 1

Pilaster

Shelf Channels

Diffuser Pan

Side toward pilaster

Side

toward

pilaster

Pilaster

Side duct

tab

Shelf channel

rear slot

Shelf channel front slot

Side duct

tab

FRONT

Installation and Start-Up

Preliminary Cleaning

Installing the Shelves

Using a suitable laboratory disinfectant, thoroughly clean all interior surfaces.

Caution Before using any cleaning or decontamination method except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Accidental spills of hazardous materials on or inside this unit are the responsibility of the user.

1. Install the pilasters, two on each side, with the tab facing into the center of the chamber with their slots up. Press downward on the top section of each pilaster to locate, then release when in position (Figure 1-9). Fit each pilaster securely into the indentations at each side of the ceiling and the floor of the chamber. Figure 1-7 shows the diffuser pan, pilasters, and shelf channels.

2. Install the diffuser pan in the bottom of the unit with

Figure 1-7. Shelf Installation

the flanges down. See Section 9 at this point if your unit includes a factory-installed datalogger (P/N 201912).

3. Install the shelf channels by placing the channel’s rear slot over the appropriate rear tab on the pilaster. Pull the shelf channel forward and engage the channel’s front slot into the pilaster’s appropriate forward tab. Refer to Figure 1-8.

Figure 1-8. Slots and Tabs

7023307 Steri-Cult 1-7Thermo Scientific

Section 1

Detail

Installation and Start-Up

Installing the Shelves

(continued)

4. Figure 1-9 shows a shelf channel installed on pilasters on the right side of the chamber.

Figure 1-9. Installed Shelf Channel

5. Slide the shelves in, side flanges upward, along the channels into the chamber.

Installing the Access Port

Filter Assembly

1. Locate the opening in the top left corner on the inside of the chamber. Remove the tape covering the opening on the outside of the unit.

2. Install the the filter/stopper assembly into the opening inside the chamber. See Figure 1-10.

Caution Filter MUST be installed inside the chamber or it will become plugged with condensation and inhibit the recovery of the RH system. Do not replace this filter with a solid cover of any kind.

1-8 7023307 Steri-Cult Thermo Scientific

Figure 1-10. Access Port Filter Installation

Section 1

Installation and Start-Up

Installing the HEPA Filter

Caution Use care when handling the filter. The media can be damaged if it

is mishandled. To avoid damage to the incubator, do not operate the unit without the HEPA filter in place. s

1. Remove the filter from the shipping box.

2. Remove the plastic covering from the filter, using caution not to touch the filter media.

3. Loosen black wingnuts in front corners of the chamber ceiling (Figure 1-11/1-12).

Figure 1-11. HEPA Orientation for Model 3307/3308

Figure 1-12. HEPA Orientation for Model 3310/3311

4. Locate the metal lip at the upper back of the chamber. Hook the HEPA filter carefully over the back edge of the metal lip.

5. Lift the front of the filter to the ceiling of the chamber. Push filter bracket back over the filter frame. Tighten the wingnuts.

6. Refer to Section 6 for HEPA filter maintenance.

7023307 Steri-Cult 1-9Thermo Scientific

Section 1

Drain fitting

Latch

Air filter

Installation and Start-Up

Leveling the Unit

Connecting the Unit to

Electrical Power

Check for level by placing a bubble-style level on one of the shelves. Turn the hex nut on the leveling feet counterclockwise to lengthen the leg or clockwise to shorten it. Level the unit front-to-back and left-to-right.

See the serial tag on the side of the unit for electrical specifications or refer to the electrical schematics in this manual.

Caution Serial tag amp rating is based on the amperage draw during the sterilization cycle. Normal operating amperage is much less. Ensure that the electrical circuit will handle the amp draw of the sterilization cycle.

Warning Connect the incubator to a grounded, dedicated circuit. The power cord connector is the mains disconnect device for the incubator. Position the incubator so the unit can be easily disconnected.

Plug the provided power cord into the power inlet connector on the back of the cabinet (See Figure 1-14), then into the grounded, dedicated, electrical circuit.

Filling the Humidity Water

Bottle

1. Open the outer chamber door.

2. Open the access door located on the front of the unit, below the control board. See Figure 1-13.

3. Remove the lid of the humidity bottle and fill with sterile distilled water. Install the lid and close the door, being careful not to pinch the tubing.

Condensation will increase water consumption. Under normal conditions of 37°C, 90% RH, 5% CO2, five door openings per day, the water bottle should last 1-1.5 weeks.

Figure 1-13. Water Bottle

For best operation of the incubator, sterilized distilled, demineralized or deionized water should be used in the humidity pan. Water purity should be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range of

20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88 for measuring water purity.

1-10 7023307 Steri-Cult Thermo Scientific

Section 1

Installation and Start-Up

Distillation systems, as well as some types of reverse osmosis water purity systems, can produce water in the quality range specified. Tap water is not recommended as it may contain chlorine, which can deteriorate the stainless steel. Tap water may also have a high mineral content, which would produce a build-up of scale in the pan. High purity or ultra pure water is not recommended as it is an extremely aggressive solvent and will deteriorate the stainless steel. High purity water has a resistance of above 1M to 18M Ohm. Even high purity water can contain bacteria and organic contaminants. Water should always be sterilized or treated with a decontaminant, safe for use with stainless steel as well as safe for the product, prior to being introduced into the humidity pan.

Use of chlorinated water, or decontamination products containing chlorine, will deteriorate the stainless steel and cause rust, voiding the warranty.

Connecting the CO2 Gas

Supply

Warning High concentrations of CO

2 gas can cause asphyxiation! OSHA

Standards specify that employee exposure to carbon dioxide in any eighthour shift of a 40-hour work week shall not exceed the eight-hour time weighted average of 5000 PPM (0.5% CO

2). The short term exposure

limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide monitors are recommended for confined areas where concentrations of carbon dioxide gas can accumulate.

Warning This incubator is designed to be operated with CO2 gas only. Connecting a flammable or toxic gas can result in a hazardous condition. Gases other than CO

2 should not be connected to this equipment. CO2

gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve. Check the gas cylinder for the proper identification labels. The CO2 gas supply being connected to the incubator should be industrial grade, 99.5% pure (nominal). Do not use CO

2 gas

cylinders equipped with siphon tubes. A siphon tube is used to extract liquid CO2 from the cylinder which can damage the pressure regulator. Consult with your gas supplier to ensure that the CO

2 cylinder does not

contain a siphon tube. Gas cylinders should also be secured to a wall or other stationary object to prevent them from tipping. A two-stage CO

2 pressure regulator is required to be installed on the outlet

valve of the gas cylinder. Input pressure to the incubator must be maintained at 15 psig (103.4 kPa), ±5 psig, for proper performance of the

2 control system. A single stage CO2 pressure regulator will not

CO maintain 15 psig (103.4 kPa) to the incubator as the pressure in the CO2 cylinder decreases; therefore, a two stage regulator is recommended. s

7023307 Steri-Cult 1-11Thermo Scientific

Section 1

Installation and Start-Up

Connecting the CO2 Gas

Supply (continued)

Warning If higher purity CO2 is desired inside the incubator (greater than

99.5% pure nominal), the pressure regulator should be constructed with a stainless steel diaphragm, along with specifying the purity of the CO

2 from

the gas supplier. Follow the manufacturer’s instructions to ensure proper and safe installation of the pressure regulator on the gas cylinder. Consult a facility safety officer to ensure that the equipment is installed in accordance with codes and regulations applicable in your area.

The CO2 gas supply being connected should be industrial grade 99.5% pure (nominal) and should not contain siphon tubes. Install a two-stage pressure regulator at the cylinder outlet. The high pressure gauge at the tank should have 0-2000 PSIG range. The low pressure gauge, at the incubator inlet, should have a 0-30 PSIG range. Input pressure to the incubator must be maintained at 15 PSIG (103.4 kPa).

The incubator has serrated fittings on the back of the cabinet to connect the gas supply. Refer to Figure 1-14. The fitting is labeled CO2 Inlet #1 Tank. Make sure that the connections are secured with clamps. Check all fittings for leaks.

Humidity and Loading the

Chamber

For units having the CO

2 Gas Guard

option, refer to Section 9.

Figure 1-14. Connection Locations

With the chamber loaded with liquid product and the RH setpoint at less than 90%, some evaporation of product will occur. If the actual RH reaches 2% above setpoint, the incubator microprocessor turns on dehumidification. This process pulls outside air in through the nebulizer and exhausts air through the access port. Humidity decreases. However, in the process, CO

2 consumption increases. Ensure your tank and product are

monitored.

1-12 7023307 Steri-Cult Thermo Scientific

Section 2 Incubator Settings

With the incubator properly installed and connected to power, the humidity water bottle filled, and the unit connected to gas supplies, system setpoints can be entered. The following setpoints can be entered in Settings mode: Temperature, Overtemp, CO2 and RH. To enter Settings mode, press the Mode key until the Settings indicator lights. Press the right and/or left arrow keys until the proper parameter appears in the message center. See Chart 2-1 for more detail.

Setting the

Temperature Setpoint

The temperature setpoint range is 10°C to 50°C, settable to within 0.1°C. The lowest temperature the incubator can control is +5°C above the ambient temperature in the incubator’s location. The incubator is shipped from the factory with a temperature setpoint of 10°C. At this setting, temperature control and alarms are turned off. To change the temperature setpoint:

1. Press the Mode key until the Settings indicator lights.

2. Press the right arrow until “Temp XX.XC” is displayed in the message center.

3. Press the up/down arrow key until the desired temperature setpoint is displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights for Run mode or press the right/left arrow keys to go to next/previous parameter.

7023307 Steri-Cult 2-1Thermo Scientific

Section 2

Incubator Settings

Setting the Overtemp

Setpoint

Caution The independent overtemp system is designed as a safety to protect

the incubator only. It is not intended to protect or limit the maximum temperature of the cell cultures or customer’s equipment inside the incubator if an overtemp condition occurs.

The Steri-Cult incubators are equipped with a secondary temperature monitoring system to monitor the air temperature inside the cabinet. This system is designed as a safety device to turn off all heaters in the event of a temperature control failure. Temperature control in the incubator will be ±1° of the overtemp setpoint.

The overtemperature is set by the factory (default) at 40°C. However, the overtemp can be set up to 55°C in 0.1° increments.

If the incubator’s operating temperature setpoint is set above the overtemp setpoint, the overtemp setpoint will automatically update to 1°C above the temperature setpoint.

It is recommended that the overtemp setpoint be maintained at 1°C over the operating temperature setpoint.

To change the Overtemp setpoint:

1. Press the Mode key until the Settings indicator lights

2. Press the right arrow until OVERTEMP XX.XC is displayed in the message center

3. Press the up or down arrow key until the desired Overtemp setpoint is displayed

4. Press Enter to save the setting

5. Press the Mode key until the Run indicator lights or press the right or left arrow to go to the next or previous parameter.

2-2 7023307 Steri-Cult Thermo Scientific

Section 2

Incubator Settings

Setting the CO2

Setpoint

Setting the Relative

Humidity (RH) Setpoint

The CO2 setpoint range is 0.0% to 20.0%, settable to within 0.1% CO2. The incubator is shipped from the factory with a CO At this setting, all CO

2 setpoint:

2 control and alarms are turned off. To change the

2 setpoint of 0.0%.

1. Press the Mode key until the Settings indicator lights.

2. Press the right arrow until “CO2 XX.X%” is displayed in the message center.

3. Press the up/down arrows until the desired CO

2 setpoint is displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights to go to Run mode or press the right/left arrow keys to go to next/previous parameter.

The RH setpoint range is 0% to 95%, settable to within 1% RH. (Settings above 90% may cause some condensation to occur in the chamber.) The incubator is shipped from the factory with an RH setpoint of 0%. At this setting, all RH control, water level control and alarms are turned off. To change the RH setpoint:

1. Press the Mode key until the Settings indicator lights.

2. Press the right arrow until “RH XX%” is displayed in the message center.

3. Press the up/down arrows until the desired RH setpoint is displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights to go to Run mode or press the right/left arrow keys to go to next/previous parameter.

On power-up or a temperature setpoint change, RH is delayed until the temperature is within 0.5°C of the setpoint. RH WAITS ON TEMP is displayed until this temperature is reached.

7023307 Steri-Cult 2-3Thermo Scientific

Section 2

Incubator Settings

2-4 7023307 Steri-Cult Thermo Scientific

Section 3 Calibration

After the unit has stabilized, several different systems can be calibrated. In Calibration mode, the air temperature, CO calibrated to reference instruments. To access Calibration mode, press the Mode key until the Calibrate indicator lights. Press the right and/or left arrow until the appropriate parameter appears in the message center. See Chart 3-1 at the end of this section for more detail.

Calibration frequency is dependent on use, ambient conditions and accuracy required. A good laboratory practice would require at least an annual calibration check. On new installations, all parameters should be checked after the stabilization period.

Prior to calibration, the user should be aware of the following system functions. While the unit is in Calibration mode, all system control functions are disabled so the unit remains stable. Readout of the system being calibrated will appear on the message center. If no keys are pressed for approximately five minutes while in Calibration mode, the system will reset to Run mode so control functions are reactivated.

2 and RH levels can be

Calibrating the

Temperature

Caution Before making any calibration or adjustments to the unit, it is

imperative that all reference instruments be properly calibrated.

Before calibration, allow the cabinet temperature to stabilize. Place the calibrated instrument in the center of the chamber. The instrument should be in the airflow, not against the shelf.

Temperature Stabilization Periods

Startup - Allow 12 hours for the temperature in the cabinet to stabilize

before proceeding.

Presently Operating - Allow at least 2 hours after the display reaches setpoint for temperature to stabilize before proceeding.

7023307 Steri-Cult 3-1Thermo Scientific

Section 3

Calibration

Temperature

(continued)

Calibrating the

Infrared CO

2 System

1. Press the Mode key until Calibrate indicator lights.

2. Press the right arrow until “TEMP CAL XX.XC” appears in the message center.

3. Press up/down arrow to match display to calibrated instrument.

4. Press Enter to store calibration.

5. Press the Mode key to return to Run or the right/left arrow to go to next/previous parameter.

All models of the Steri-Cult incubator have an infrared (IR) CO Infrared CO

2 sensors are not effected by chamber atmosphere temperature

2 sensor.

or humidity.

2 Sensor Stabilization Times

Start-Up- Allow the CO

2 of the cabinet to stabilize at least 12 hours before

proceeding.

Presently Operating - Allow CO

2 to stabilize at least 2 hours at setpoint

before proceeding.

1. Measure the CO

2 concentration in the chamber through the gas sample

port with a Fyrite or other independent instrument. Several readings should be taken to ensure accuracy.

2. Press the Mode key until the Calibrate indicator lights.

3. Press the right arrow until “CO2 CAL XX.X%” appears in the message center.

4. Press the up/down arrow to adjust the display to match the independent instrument reading.

5. Press Enter to store the calibration.

6. Press the Mode key to return to Run mode.

3-2 7023307 Steri-Cult Thermo Scientific

Section 3

Calibration

Calibrating Relative

Humidity (RH)

Place an accurate independent instrument in the center of the chamber. Most RH measuring devices are least accurate above 90%. It is recommended that when calibrating above 90%, the incubator should be adjusted to the lower edge of the instrument accuracy range. For example; with the incubator set at 95%, the independent instrument with accuracy of ±5% reads 94%. Calibrate the incubator display to 99%. (This prevents condensation issues caused by actual RH levels above 95%.)

Relative Humidity Stabilization Times

Start-Up - Allow 12 hours for the relative humidity and temperature in the

chamber to stabilize before proceeding.

Presently Operating - Allow at least 2 hours after temperature display reaches setpoint for relative humidity to stabilize before proceeding.

1. Press the Mode key until the Calibrate indicator lights.

2. Press the right arrow key until “RH CAL XXX%” appears in the message center.

3. Press the up/down arrow to match the display to the independent instrument.

4. Press Enter to store the calibration.

5. Press the Mode key to return to Run mode.

7023307 Steri-Cult 3-3Thermo Scientific

Section 3

Calibration

3-4 7023307 Steri-Cult Thermo Scientific

Section 4 Configuration

Several features available in the Configuration Mode allow custom setup of the incubator. These features are listed and described below. All features may not be necessary in all applications, but are available if needed. To enter Configuration mode, press the Mode key until the Configuration indicator lights. Press the right and/or left arrow until the appropriate parameter appears in the message center. See Chart 4-1 for more detail.

Turning the Audible

Alarm ON/OFF

New HEPA Filter

The audible alarm can be turned on or off. The factory setting is ON.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until AUDIBLE ON/OFF is displayed in the message center.

3. Press up/down arrow to toggle ON or OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

When the REPLACE HEPA reminder is displayed and the visual alarm flashes, the specified time has elapsed and the HEPA filter should be replaced.

After replacing the HEPA filter with a new one, clear the display and reset the timer by following the steps below.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until NEW HEPA XXX is displayed in the message center.

3. Press Enter to restart the timer and clear the REPLACE HEPA alarm.

4. Press the Mode key to return to Run mode.

7023307 Steri-Cult 4-1Thermo Scientific

Section 4

Configuration

Setting the REPLACE

HEPA Filter Reminder

A HEPA filter replacement timer can be set for a specific amount of time, from 1 to 12 months of actual unit running time. Time will not accrue when the unit is turned off. The default time is 6 months. When the allotted time has run out, REPLACE HEPA appears in the display and the visual alarm flashes. To set the reminder, use the following procedure.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until REPLACE HEPA XX is displayed.

3. Press the up/down arrow to choose the number of months desired.

4. Press Enter to save the number.

5. Press the Mode key to return to Run Mode or right/left to go to next/previous parameter.

Note After the reminder has been set, check the allotted time remaining by going to Configuration mode, then pressing the right arrow until NEW HEPA XXX displays. This number is the remaining days before the filter replacement time specified runs out. For example, if 12 months was chosen in the REPLACE HEPA XX message screen, the NEW HEPA number would be 365 days.

Setting an Access

Code

4-2 7023307 Steri-Cult Thermo Scientific

A 3-digit Access Code can be entered to avoid unauthorized personnel from changing the setpoints, calibration, or configuration. A setting of 000 disables the access code function. The factory setting is 000.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until ACCESS CODE XXX is displayed in the message center.

3. Press up/down arrow to change the access code.

4. Press Enter to save the access code.

5. Press the Mode key to return to the Run mode or right/left to go to next/previous parameter.

Section 4

Configuration

Setting a Low Temp

Alarm Limit

Enabling Temp Alarms

to Trip Contacts

The low temperature alarm limit (tracking alarm) is the deviation from the temperature setpoint which causes a low temp alarm. The low temp alarm is variable from one-half degree to five degrees below setpoint. The factory setting is 1° below setpoint. A minus sign (-) in the display indicates that the alarm setting is below the setpoint.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until TEMP LO LMT -X.X is displayed in the message center.

3. Press up/down arrow to change the low temp alarm limit.

4. Press Enter to save the low temp alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

The high and low temperature alarms can be programmed to trip the remote alarm contacts. A setting of ON activates this ability. A setting of OFF will not allow the temp alarms to trip the contacts. The factory setting is ON.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until TEMP RELAY ON/OFF is displayed.

3. Press the up/down key to toggle the setting ON/OFF.

4. Press Enter to save the setting

5. Press the Mode key to return to Run or the right/left arrow key to go to next/previous parameter.

7023307 Steri-Cult 4-3Thermo Scientific

Section 4

Configuration

Setting a Low CO2

Alarm Limit

Setting a High CO2

Alarm Limit

The low CO2 alarm limit (tracking alarm) is the deviation from the CO2 setpoint which will cause a low CO2 alarm. The setpoint is variable from

0.5% to 5.0% CO

2 below setpoint. The factory setting is 1.0% CO2

below setpoint. A minus sign (-) in the display indicates that the alarm setting is below the setpoint.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until CO2 LO LMT -X.X is displayed in the message center.

3. Press up/down arrow to change the low CO

4. Press Enter to save the low CO

2 alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

The high CO

2 alarm limit (tracking alarm) is the deviation from the CO2

setpoint which will cause a high CO2 alarm. The setpoint is variable from

0.5% to 5.0% CO

setpoint. When activated, the CO

2 above setpoint. The factory setting is 1.0% CO2 above

2 valve is disabled.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until CO2 HI LMT X.X is displayed in the message center.

3. Press up/down arrow to change the high CO

4. Press Enter to save the high CO

2 alarm limit.

5. Press the Mode key to return to run mode or right/left to go to next/previous parameter.

4-4 7023307 Steri-Cult Thermo Scientific

Section 4

Configuration

Enabling CO

2 Alarms

to Trip Contacts

Setting a Low RH

Alarm Limit

High and Low CO2 alarms can be programmed to trip the remote alarm contacts. A setting of ON activates this ability. A setting of OFF will not allow CO

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until CO2 RELAY ON/OFF is displayed in the

3. Press up/down arrow to toggle the setting ON or OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to

The low RH alarm limit (tracking alarm) is the deviation from the humidity setpoint which causes a low RH alarm. The setpoint is variable from -5 to -20% RH. The factory setting is -10%RH.

2 alarms to trip the contacts. The factory setting is ON.

message center.

next/previous parameter.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until RH LO LMT -XX is displayed in the message center.

3. Press up/down arrow to change the RH low alarm limit.

4. Press Enter to save the RH low alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

7023307 Steri-Cult 4-5Thermo Scientific

Section 4

Configuration

Setting a High RH

Alarm Limit

Enabling RH Alarms

to Trip Contacts

The high RH alarm limit (tracking alarm) is the deviation from the humidity setpoint which causes a high RH alarm. The setpoint is variable from 5 to 20% RH The factory setting is 10% RH. When the alarm is activated, the humidity system is disabled.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until RH HI LMT XX is displayed in the message center.

3. Press up/down arrow to change the RH high alarm limit.

4. Press Enter to save the RH high alarm limit.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

The high and low RH alarms can be programmed to trip the remote alarm contacts. A setting of ON activates this ability. A setting of OFF will not allow the RH alarm to trip the contacts. The factory setting is ON.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until RH RELAY ON/OFF is displayed in the message center.

3. Press up/down arrow to toggle the setting ON or OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

4-6 7023307 Steri-Cult Thermo Scientific

Section 4

Configuration

Selecting a Primary

Tank

Disabling the Gas

Guard System

On units equipped with the Gas Guard option, a primary tank can be selected. The primary tank will be either Tank 1 or 2. The factory setting is Tank1.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until TANK SELECT X is displayed in the message center.

3. Press up/down arrow to toggle setting between 1 and 2 .

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

On units equipped with the Gas Guard option, the Gas Guard system may be turned ON, or OFF if it is not in use. The factory setting is ON.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until GAS GUARD ON/OFF is displayed in the message center.

3. Press up/down arrow to toggle the setting ON or OFF.

4. Press Enter to save the setting.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

7023307 Steri-Cult 4-7Thermo Scientific

Section 4

Configuration

Setting a RS485

Communications Address

On units that have the RS485 option, direct communication with the Model 1535 alarm system can be established. Each piece of equipment connected to the Model 1535 must have a unique address. An address of 0-24 can be entered for the incubator. A setting of 0 is an invalid address that the 1535 will ignore. The factory setting for the RS485 address is 0.

1. Press the Mode key until the Configuration indicator lights.

2. Press the right arrow until 485 ADDRESS XX is displayed in the message center.

3. Press up/down arrow to move the RS485 address.

4. Press Enter to save the RS485 address.

5. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

4-8 7023307 Steri-Cult Thermo Scientific

Section 4

Configuration

7023307 Steri-Cult 4-9Thermo Scientific

Section 4

Configuration

4-10 7023307 Steri-Cult Thermo Scientific

Section 4

Configuration

7023307 Steri-Cult 4-11Thermo Scientific

Section 5 Alarms

The Steri-Cult incubator alarm system is shown in the table below. When an alarm is active, the message appears in the LED message center. Pressing Silence disables the audible alarm for the ringback period. However, the visual alarm continues until the incubator returns to a normal condition. The alarms are momentary alarms only. When an alarm condition occurs and then returns to normal, the incubator automatically clears the alarm condition and the message center.

Table 5-1. Alarms and Descriptions

Description Message Delay Ringback Relay

No alarm condition exists

Temp > OVERTEMP Setpoint SYSTEM IN OTEMP 0 min. 15 min. Yes Temperature Controller Failure TEMP CNTRL ERROR 0 min. 15 min. Yes Air Temp Sensor Fault AIR SENSOR ERROR 0 min. 15 min. No CO2 Sensor Fault CO2 SENSOR ERROR 0 min. 15 min. No RH Conditioner Sensor Fault RH COND SNSR ERR 0 min. 15 min. No Water Bottle Empty WATER EMPTY 5 min. 15 min. No Inner Door is Open DOOR IS OPEN 15 min. 15 min. No CO2 > CO2 High Tracking Alarm CO2 IS HIGH 0 min. 15 min. Prog. CO2 < CO2 Low Tracking Alarm CO2 IS LOW 15 min. 15 min. Prog. No water flow through the water valve NO WATER FLOW 5 min. 15 min. No TEMP < TEMP Low Tracking Alarm TEMP IS LOW 15 min. 15 min. Prog. RH < RH Low Limit Alarm RH IS LOW 30 min. 15 min. Prog. RH > RH High Limit Alarm RH IS HIGH 0 min. 15 min. Prog. RH System Fault CHECK RH SYSTEM 60 min. 15 min. No Change HEPA filter reminder-set time expired* CHANGE HEPA SOON 0 min. ------- No RH Conditioner Failure RH COND HEAT ERR 60 min. 15 min. No Tank1 is low, switch to tank 2 (Gas Guard only) TANK 1 LOW 0 min. N/A No Tank2 is low, switch to tank 1 (Gas Guard only) TANK 2 LOW 0 min. N/A No Tank 1 and 2 are low (Gas Guard Only) TANK 1&2 LOW 0 min. 15 min. No Water Bottle is Low* WATER LOW 0 min. N/A No

SYSTEM OK, CLASS 100

or RH WAITS ON TEMP

------- ------- -------

* Visual only, no audible alarm -All alarm delays and ringback times are ±30 seconds-

When multiple alarm conditions occur, active messages display in the message center one at a time, updating at 5 second intervals. Pressing Silence during multiple alarms causes all active alarms to be silenced and ringback in 15 minutes.

The temperature alarms are disabled when the temperature setpoint is 10°C. The CO

2 alarms are disabled when the CO2 set point is 0.0%. RH

alarms are disabled when the setpoint is 0% RH.

7023307 Steri-Cult 5-1Thermo Scientific

Section 5

Alarms

Temp Controller Failure

TEMP CNTRL ERROR

RH System Fault

In addition to other safety features designed into Steri-Cult incubators, a thermostat is provided to monitor the cabinet temperature. In the event of a temperature control failure, the thermostat will turn off all heaters at a cabinet temperature of 160°C (±5%). This is intended to be a safety feature to protect the incubator, and is not intended to protect the cell cultures or the equipment inside the chamber should a temperature control failure occur. Should such a failure occur, contact the Technical Services Department or your local distributor.

This alarm occurs if the humidifier has been detected as being on for an abnormal length of time. Possible causes are:

1. RH calibrated too low.

2. Humidifier tubing pinched or disconnected.

3. Access port is plugged.

4. Ultrasonic humidifier has heavy build-up. See Section 6, Cleaning the Humidity System.

Sensor Fault Alarms

Gas Guard Alarms

5. If none of the above, contact the Technical Services department.

The microprocessor in Steri-Cult incubators continually scans all available sensors to ensure that they are operating properly. Should an error be detected, the incubator sounds an alarm and displays the appropriate message. Contact the Technical Services Department or your local distributor.

If the Gas Guard option is installed, the choice of tank is available in Configuration mode. If the tank in use goes low, the unit automatically switches to the other tank. However, an audible alarm and warning light activate indicating the low tank condition. Pressing Silence clears the alarm condition and does not ringback. The warning light on the display remains activated until the tank is replaced and both tanks are normal.

If the tank not in use goes low, the warning light is activated. If both banks go low, the audible alarm and warning light are activated.

5-2 7023307 Steri-Cult Thermo Scientific

Section 5

Alarms

Water Level Alarms

Door Open Alarm

When the water level in the bottle falls to approximately 3 inches (7.6cm), a visual alarm with display message “WATER LOW” occurs. Pressing Silence clears the alarm condition and does not ringback. The blue backlight on the water bottle flashes until the bottle is filled.

If the water bottle fill switch is empty/low and the humidity reservoir fill switch is empty/low, an audible alarm with display message “WATER EMPTY” occurs. Pressing Silence mutes the audible alarm but it rings back in 15 minutes. The blue backlight on the water bottle flashes until the bottle is filled.

If the water level inside the humidification system is not as required, yet the water level in the bottle is not low, then an audible alarm with display message “NO WATER FLOW” occurs. Pressing Silence mutes the audible alarm but it rings back in 15 minutes until the condition is resolved.

When the door is opened on the Steri-Cult incubator, heat and CO

injection are disabled. The door must be securely latched for heat and CO2 injection to resume after a door opening.

If the door is latched, yet the display still shows “Door Ajar”, the door switch could be faulty. Call Technical Services.

7023307 Steri-Cult 5-3Thermo Scientific

Section 6 Troubleshooting

Certain conditions can occur in usage of this incubator which can be resolved by checking the troublshooting chart below. If the issue is not listed or cannot be resolved, contact the Services department.

Table 6-1. Troubleshooting Guide

Problem Possible Cause Solution

Poor temperature recovery and/or temper-

ature overshoot

HEPA filter is dirty

Temperature probe not reinstalled correctly.

If the HEPA filter is older than six months,

replace with new.

Ensure temperature probe holder bottoms-out

in hole.

Poor CO2 recovery and/or actual higher

than display

Poor RH recovery Access port is plugged.

Condensation on cabinet face Removable gasket not reinstalled properly.

Condensation on cabinet interior RH out of calibration. Calibrate the RH, see Section 3.

CO2 sensor not reinstalled correctly. Ensure CO2 sensor bottoms-out in hole.

Install access port filter assembly. If it is older

than six months, replace with new.

Humidifier tubing pinched or disconnected.

Humidifier has heavy build-up. Clean the humidifier; see Section 6.

RH sensor not reinstalled correctly. Ensure RH sensor bottoms out in hole.

RH sensor not reinstalled correctly. Ensure RH sensor bottoms-out in hole.

Check to ensure tubing is not pinched or dis-

connected.

Ensure there are not gaps between the gasket

and the glass door.

7023307 Steri-Cult 6-1Thermo Scientific

Preventive Maintenance for Steri-Cult Incubators

Refer to Manual

Section

Action Daily Weekly Monthly 6

Months

Yearly

-- Check CO

tank levels.

-- Perform preventive maintenance per instruction sheet 7270102 using PM kit #2270102

2 * Verify and document CO2, humidity and temperature

calibration, as applicable.

6 Disinfect the interior of the incubator.

-- Replace AquaTec water cleaning cell, if applicable.

7 Perform Sterilization Cycle as needed.

PREVENTIVE MAINTENANCE

Incubators

Your equipment has been thoroughly tested before shipment. Regular preventive maintenance is important to keep your unit functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For maximum performance and efficiency, it is recommended the unit be checked and calibrated periodically by a qualified service technician.

The following is a condensed list of preventive maintenance requirements. See the specified section of the instruction manual for further details.

We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on Preventive Maintenance or Extended Warranties, please contact us at the number listed below.

Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required.

Tips for all incubators:

x Do NOT use bleach or any disinfectant that has high chloros x Use sterile

, distilled or demineralized water.

x Avoid spraying cleaner on the CO

sensor.

x Do not use powdered gloves for tissue cultures.

6-2 7023307 Steri-Cult Thermo Scientific

Section 7 Routine Maintenance

Warning If the unit has been in service, turn it off and disconnect the

power cord connector before proceeding with any maintenance.

Cleaning the

Incubator Interior

Caution Before using any cleaning method except those recommended by

the manufacturer, users must check with the manufacturer that the proposed method will not damage the equipment.

Use an appropriate disinfectant. All articles and surfaces must be thoroughly cleaned, rinsed with sterile water, and rough-dried.

Warning Alcohol, even a 70% solution, is volatile and flammable. Use it only in a well ventilated area that is free from open flame. If any component is cleaned with alcohol, do not expose the component to open flame or other possible hazard.

Warning Do not use strong alkaline or caustic agents. Stainless steel is corrosion resistant, not corrosion-proof. Do not use solutions of sodium hypochlorite (bleach) as they may also cause pitting and rusting.

A HEPA filter replacement kit (see parts list section) should be on hand prior to cleaning the interior of the incubator.

1. Remove the shelves, access port filter assembly, HEPA filter and the left and right pilasters. Discard the HEPA filter and the access port filter assembly.

2. Wash the shelves and pilasters with disinfectant, then rinse with sterile water. Optional: The shelves and pilasters may be autoclaved.

3. Wash the inner door gasket with disinfectant. This gasket may be removed to be cleaned, or replaced.

7023307 Steri-Cult 7-1Thermo Scientific

Section 7

Routine Maintenance

Cleaning the Interior

(continued)

4. Wash the cabinet interior with disinfectant, starting at the top and working down. Refer to the disinfectant label for instruction on the length of time needed before rinsing. Wash the inner door(s) both inside and out. The cabinet and door must be rinsed with sterile water until the disinfectant has been removed. After the cabinet has been rinsed, spray with 70% alcohol.

Some precautions in the cleaning and care of the incubator glass doors:

Moisture leaches alkaline materials (sodium, Na) from the surface of the glass. Evaporation of the moisture concentrates the alkaline and may produce a white staining or clouding of the glass surface. Cleaning chemicals with a PH above 9 and heat (autoclaving) accelerate the corrosion process. Therefore, it is very important to rinse and dry the glass doors after cleaning. Autoclaving the glass doors should be avoided. There is no simple method for repairing corroded glass. In most cases, the glass must be replaced.

5. Install the left and right pilasters, inner door gasket and access port filter assembly, spraying each with 70% alcohol.

6. Install a new HEPA filter.

Cleaning the Cabinet

Exterior

Cleaning the Humidity

System

7. Install the shelves and spray with 70% alcohol.

Clean the incubator exterior with a damp sponge or soft, well-wrung cloth and mild detergent dissolved in water. Dry with a soft cloth.

Make sure power is on and the RH setpoint has been changed from factory default.

1. Open outer chamber door.

2. Open the access door located on the front of the unit, below the control panel.

3. Locate the humidity system drain fitting attached to the bottom of this door. See Figure 1-13.

4. Install the hose barb end of this fitting to the supplied tubing and route to a drain or suitable 0.5 gallon (3.8 liter) container.

7-2 7023307 Steri-Cult Thermo Scientific

Section 7

Bot

Press inward

Disengage

Routine Maintenance

Cleaning the Humidity

System (continued)

5. Connect the other end of the fitting to the quick-connect drain located below the fold-down storage door. See Figure 7-1. Allow water to drain. This may take several minutes.

Figure 7-1. RH Drain

Figure 7-2. Drain Water Bottle

6. Turn power off.

7. Attached to the bottom of the water bottle is a length of tubing and low voltage wiring (Figure 7-2). Disconnect the tubing by pressing inward on the collar where the tubing connects to the bottle. Pull the tubing free. Disengage the wiring at the connector.

8. Lift the water bottle from the bottle platform. Empty any water out of the bottle.

9. Replace the filter in the lid by first unscrewing the clear plastic nut on the outside of the lid. Catch the filter as it loosens from the inside of the lid. Remove the small black o-ring from the filter and discard the filter. Assemble this o-ring onto the new filter and install as previously. Tighten the plastic nut only finger-tight to

Figure 7-3. Filter Assembly

prevent stripping.

9. Clean the humidity bottle and lid with soap and water and a generaluse laboratory disinfectant. Rinse with sterile water and spray with 70% alcohol.

7023307 Steri-Cult 7-3Thermo Scientific

10. Also behind the access door is the humidifier. See Figure 7-4. Lift the latches on both sides of this small unit.

11. Separate the top and bottom sections. Carefully clean the inside of both sections and spray with 70% alcohol.

Caution Do not touch the metal disk in the bottom of the humidifier with bare fingers as any fingerprints or other residue can affect humidity recovery.

12. Reinstall the top section of the humidifier. Fasten the latches on each side.

13. Reconnect the tubing and wiring to the water bottle as previously.

1. Locate the humidity system drain fitting attached to the inside of the water bottle access door. See Figure 7-5.

2. Install the hose barb end of this fitting to the supplied tubing and route to a drain or suitable 0.5 gallon (3.8 liter) container.

3. Connect the other end of the fitting to the quick-connect drain located below the fold-down storage door (Figure 7-2). With the incubator turned On, allow the entire humidity system to drain. This could take several minutes.

4. Prepare 0.5 gallon (3.8 liters) of decontamination solution per the instructions on the container. Sudsy ammonia works well to kill waterborne contaminants, such as pseudomonas.

5. Disconnect the drain fitting.

6. Fill the incubator water bottle with the decontamination solution. Change the incubator humidity system setpoint to 95% (Section 2).

7-4 7023307 Steri-Cult Thermo Scientific

Section 7

Routine Maintenance

Flushing the Humidity

System

Figure 7-4. Humidifier

Cleaning the Humidity

System (continued)

Figure 7-5. Water Bottle

Air filter

Latch

Drain fitting

7. Open both doors to the incubator. Tape down the door switch (Figure 7-6) on the face of the unit. This ensures the humidity nebulizer operates in a 8 seconds on, 2 seconds off continuous cycle.

8. Allow the incubator to run approximately 30 minutes to fill and agitate the solution in the humidity system.

9. Reconnect the drain fitting and drain the system again.

10. Disconnect the drain fitting and fill the incubator water bottle with distilled water. Allow the incubator to run for 15 minutes.

11. Connect the drain fitting and allow the system to drain.

12. Repeat the Steps 9 and 10 until all decontaminant is rinsed from the incubator humidity system.

13. Remove the tape from the door switch. Fill the humidity system per the instructions in Section 1 and return the unit to service.

7023307 Steri-Cult 7-5Thermo Scientific

Section 7

Routine Maintenance

Flushing the Humidity

System (continued)

Figure 7-6. Door Switch

Replace the HEPA filter when the REPLACE HEPA reminder is displayed, or after a Sterilization cycle.

Caution The HEPA filter must be removed before the Sterilization cycle. The filtering material cannot withstand the 140° heat. It will melt onto the shelves and be difficult to remove.

The REPLACE HEPA reminder can be set to alarm after a specified time from 1 to 12 months. The reminder default is the factory recommended setting of 6 months. For details on setting the reminder, see Section 4.

Replace the HEPA filter (Figures 7-7a and 7-7b) using the steps below.

1. Locate and loosen the two large wingnuts in the top of the inner chamber. Slide the filter bracket toward the chamber opening until the filter swings down.

2. Lift the back edge of the HEPA filter until it unhooks from the metal lip in the back of the chamber. Discard the filter.

3. Install a new HEPA by first hooking the back edge of the filter as previously.

4. After the back edge is hooked, lift the front of the HEPA to the top of the chamber. Slide the filter bracket over the HEPA frame to secure it. Tighten the two wingnuts.

7-6 7023307 Steri-Cult Thermo Scientific

Section 7

Routine Maintenance

Figure 7-7a. HEPA Orientation for 3307/3308

Figure 7-7b. HEPA Orientation for 3310/3311

HEPA Filter

Maintenance

Section 8 Sterilization Cycle

Sterilization Cycle

Information You Need to Know Before Starting a Cycle

• If the Sterilization Cycle is to be performed on initial start-up with new unused filters, do not install the access port or HEPA filters until AFTER the Sterilization Cycle. If the unit has been in service, the HEPA must be removed BEFORE the Sterilization cycle. The filtering material cannot withstand the 140° heat. It will melt onto the shelves and be difficult to remove.

• The Sterilization Cycle requires approximately 14 hours - heat-up (2.5-5 hours), sterilize (2 hours), and cool down (8-10 hours). Additional time is needed to verify the calibration of temperature and

2 after the cycle is complete.

• During the Sterilization Cycle, the incubator updates the temperature to the analog output board and the 1535, however CO

2 and RH will be fixed at setpoint.

• A HEPA filter replacement kit (see the spare parts list) should be on hand prior to initiating the Sterilization Cycle.

Information About the Cycle

• Pre-cleaning may be required. In order to avoid odors, stains on the interior, baked-on material, etc., wipe off all visible signs of spills.

• Odor may occur during the Sterilization Cycle and is considered normal.

• The Sterilization Cycle is not intended to sterilize other items; instruments, etc., from the lab.

• During the cycle, the unit chamber becomes hot enough to melt samples, instruments, dishes, etc., left inside the unit. The RH and CO2 sensors also need to be removed.

• During the Sterilization Cycle, discoloration of some materials may occur. For example, stainless steel turns a straw color after a period of exposure to high temperatures. This is normal.

7023307 Steri-Cult 8-1Thermo Scientific

Section 8

Sterilization Cycle

Table 8-1. Checkpoints

What if? Then

Cycle does not initiate or terminates in mid-cycle

Units are stacked

Check for alarms: TEMP IS HIGH, AIR SENSOR ERROR, TEMP CNTRL

ERROR

A Sterilization Cycle performed on either unit affects performance on second unit. Do not use second unit during Sterilization Cycle. The Sterilization Cycle may be performed on both units simultaneously, with proper electrical connection.

No action is taken within 10 minutes, following REMOVE HEPAs display

prompt

No action is taken within 5 minutes, following REMOVE SENSORS display

prompt

Unit returns to normal operation, SYSTEM OK

Need to cancel cycle in progress Hold down Sterilization Cycle initiation button for 3 seconds

The cycle will not continue. REMOVE SENSORS shows in the dis-

Sensor hole plugs are not installed

play. If not installed within 5 minutes, unit returns to normal operation, SYSTEM OK.

The outer door is opened during the Heat or Sterilization phases

An outer door alarm occurs: CLOSE DOOR in the display, plus an audible (cannot be silenced) and visual alarm.

The outer door is open longer than 20 seconds during phases listed above Cycle is cancelled, unit goes to CANCELLED COOL PHASE*

The outer door is open during the Cool Down phase when the temp is

60°C or greater

Power interruption during the HEAT PHASE

An outer door alarm occurs (cannot be silenced)

HEAT PHASE resumes if the chamber temperature was less than 90°C when power was interrupted

HEAT PHASE resumes if the chamber temperature was greater than 90°C when power was interrupted, and the temperature dropped less than 1°C

CANCELED COOL PHASE* starts if the chamber temp drop is more than 1°C

Power interruption during the STERILIZATION PHASE

Sensor hole plugs are not removed at the end of the cycle.

Sensor hole plugs are removed, but sensors are not reinstalled correctly.

* CANCELED COOL PHASE - The display toggles between “CYCLE CANCELLED” and “COOL PHASE”

When the temperature cools down to the original set operating temperature or 30°C, whichever is higher, the display toggles between “CYCLE CANCELLED”, “REPLACE HEPAs”, “REPLACE SENSORS”, and “PRESS ENTER”. The green LED is lighted but no longer flashes. After the unit is powered back on with the CO sensor reinstalled, the display returns to “SYSTEM OK”.

8-2 7023307 Steri-Cult Thermo Scientific

HEAT PHASE begins if the chamber temperature has not dropped

below 139°C

STERILIZATION PHASE starts again when the chamber temperature

reaches 140°C

CANCELED COOL PHASE* starts if the chamber temp dropped below

139°C

INSTALL SENSORS is displayed until the sensor hole plugs are removed. All control is delayed for 1 minute to allow time to reinstall the sensors.

RH level in chamber reaches saturation and CO2 level becomes dangerously high.

Section 8

Sterilization Cycle

Warning The Sterilization Cycle will heat the incubator interior surfaces to

140°C. Contact with any surface inside the outer door during this cycle may result in burns.

1. Remove any samples, instruments, dishes, etc.from the chamber. Set the inventory system magnet plate aside, if used.

2. Press and hold the Sterilization Cycle initiation button on the control panel (Figure 8-1) for approximately 3 seconds until it lights.

Figure 8-1. Sterilization Cycle Initiation Button

3. Enter the access code, if applicable. An access code is recommended to prevent accidental cycle initiation.

4. Pre Sterilization - The display toggles between “REMOVE HEPAs” and “PRESS ENTER”. If no response within 10 minutes, the display returns to “SYSTEM OK”. If the filter is removed (refer to Section 7, HEPA Filter Maintenance) and Enter is pressed within 10 minutes, the display begins to toggle between “OPEN ACCESS DOOR”, “REMOVE SENSORS” and “PRESS ENTER”. If no response within 5 minutes, the display returns to “SYSTEM OK”.

5. Remove the RH and CO

2 sensors.

To access the sensors, open the access door containing the humidity system water bottle. (The illustration shows the bottle removed for clarity.) Locate the RH and CO

2 sensors (Figure 8-2).

Figure 8-2. Hole Plugs and Sensors

6. Pull the sensors outward carefully. Allow them to hang. See Step 12 for the sensor disinfecting procedure.

7. Secured in the same area are sensor hole plugs. Install a plug into each sensor location. Close the access door.

8. After ENTER is pressed with the sensor hole plugs installed, the Heat Phase initiates. The cycle initiation button flashes and the display toggles between “STERILIZING” and “HEAT PHASE”. During this period, the HEAT light will be on and the cabinet heats to sterilization temperature.

7023307 Steri-Cult 8-3Thermo Scientific

Section 8

Sterilization Cycle

(continued)

Figure 8-3. Heat Phase in Sterilization Cycle

9. When the air temperature in the unit reaches 140.0°C, the Sterilization Phase begins and the display shows “STERILIZING”.

10. After approximately 2 hours, a 5-second audible tone sounds, signaling that sterilization is complete. The Cool Phase begins. The display toggles between “STERILIZING” and “COOL PHASE”.

8-4 7023307 Steri-Cult Thermo Scientific

Figure 8-4. Cool Phase in Sterilization Cycle

Section 8

Sterilization Cycle

(continued)

11. Completed Sterilization Cycle - When the temperature cools down to the original set operating temperature or 30°C, whichever is higher, the display toggles between “CYCLE COMPLETE”, “REPLACE HEPAs”, “INSTALL SENSORS”, and “PRESS ENTER”. The cycle initiation button is lighted but no longer flashes.

Figure 8-5. Cycle Complete

12. Disinfect the RH and CO

2 sensors with either isopropanol or Lysol

No-Rinse Sanitizer. Spray cleaner on a clean, soft cloth and wipe each sensor.

Caution Do not saturate the sensors or immerse the sensors in a cleaner.

13. Open the side panel, and replace the sensor hole plugs into their clips. Install the cleaned RH and CO

2 sensors in their respective holes. Be

sure the sensors reach the hole limit.

Caution RH and CO properly sense chamber conditions! If not, RH and CO uncontrolled.

2 sensors must be reinstalled correctly for systems to

2 levels may be

7023307 Steri-Cult 8-5Thermo Scientific

Section 8

Sterilization Cycle

(continued)

14. Open the chamber and replace the present HEPA with a new one. For instructions to install, see Section 1.

15. Install the new access port filter assembly into the upper left corner of the back of the chamber (Section 1). Press ENTER.

16. When ENTER is pressed, the cycle initiation button goes out, the display returns to “SYSTEM OK”, and the HEPA filter replacement timer is reset.

17. Reinstall the inventory system magnet plate, if used.

18. Allow to stabilize for at least 2 hours at the required temperature and

2 level.

19. Verify the calibration of the sensors. See Section 3, CO

2 or RH.

Note The sterilization process can cause the inner door knob to become difficult to turn. A small amount of mineral oil, vegetable oil or similar oil applied to the knob should remedy this.

8-6 7023307 Steri-Cult Thermo Scientific

Section 9 Factory Installed Options

Information about connecting to external equipment follows.

Remote Alarm

Contacts

A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet. Refer to Figure 9-2 for the location of the alarm connector. The 12-foot telephone cord (P/N

190388) and RJ11-to-screw terminal conversion box (P/N 190392) are available through our Services department.

The remote alarm provides a NO (normally open) output, an NC (normally closed) output and COM (common). Refer to Figure 9-1.

The contacts will trip on a power outage or a temperature controller failure. The contacts may also be programmed to trip or not trip on temperature alarms, CO Configuration.

2 alarms, and RH alarms. See Section 4,

Figure 9-1. Remote Alarm Outputs

7023307 Steri-Cult 9-1Thermo Scientific

Section 9

Factory Options

RS485 Interface

All Steri-Cult incubator models can be purchased with the RS485 communications option (P/N

1900152). This option allows the incubator to be

directly connected to a Model 1535 alarm system without the use of a communications module. A junction box is provided with each RS485 option. See Figure 9-3 for wiring details. Figure 9-2 shows the location of the RS485 connector on the back of the incubator cabinet.

To allow the incubator and the 1535 to communicate, an address must be allocated on the

1535. Refer to Section 5.8 of the 1535 operating

manual. The same address number must be assigned to the incubator. Refer to Section 4 of this manual.

Figure 9-2. RS485 Connector location

Figure 9-3. RS485 Wiring

9-2 7023307 Steri-Cult Thermo Scientific

Section 9

Factory Options

Connecting the

Analog Output Boards

Warning The electronics section contains hazardous voltages. Only

qualified personnel should access this area.

The analog output board (P/N 191761, 191762, 191763) is an option that allows the incubator to output analog signals representing the air temperature of the cabinet, CO

2 content, and relative humidity, depending

upon which systems are in the incubator. There are three different analog output board options available : 0-1V, 0-5V, or 4-20mA signals. Negative display readings will output 0V. The outputs do not have isolated grounds. Refer to Table 9-1 for output specifications of the three boards.

For the 0-1V and 0-5V boards, the recording device must supply a load > 1000 ohm. For the 4-20mA board, the recorder must supply a load of < 100 ohm.

To wire in the analog output board, use a shielded 22 gauge, 3-conductor wire (P/N 73041), maximum length 50 ft (15.2m). This wire is readily available from other vendors including Alpha P/N 2403 and Deerborn P/N 972203.

Caution Accuracy of the output at the board terminal strip to the incubator display is ±1 unit. There is no calibration from the incubator. Calibration to the incubator display must be at the instrument connected to the output board.

Table 9-1. Analog Output Board Specifications

191761 4-20 mA Output Scaling 4-20mA Equals

Temperature 12.5-162.5°C 0.0-150.0°C 0.0-150.0°C

RH 0-100 %RH 0-100 %RH 0-100 %RH

CO2 0.0-100.0 %CO2 0-100.0 %CO2 0-100.0 %CO2

191763 0-1V Output Scaling 0-1 V Equals

191762 0-5V Output Scaling 0-5V Equals

7023307 Steri-Cult 9-3Thermo Scientific

Section 9

Factory Options

Connecting Analog Boards (continued)

To install the analog board:

1. Turn the incubator Off and disconnect it from the power source.

2. Remove the two screws from the bottom of the left side panel to access the electronics area. Using the handle provided, lift the side panel up and out.

3. On the left side of the opening is the electronics area. Locate the Analog Output board.

4. Each system monitored (Temp, CO2, RH) requires two conductors (Refer to Figure 9-4).

Figure 9-4. Conductors on Analog Output Board

5. Feed the wires through the auxiliary wire through-port (Figure 9-5) on the back panel. This port is a circular fitting adjacent to the remote alarm and RS485 connectors.

6. Strip the ends of each conductor and wire it to the appropriate terminals of connectors J2 and/or J3 on the analog board. Refer to Figure 9-4.

7. When wiring is completed, install the side panel and secure with the screws. Return the unit to service.

Auxiliary Wire Through-port

Figure 9-5. Through-port

9-4 7023307 Steri-Cult Thermo Scientific

Section 9

Factory Options

CO2 Gas Guard

Connecting the CO2 Gas

Supplies

Warning CO2 can be lethal in high concentrations. Refer to warnings in

Section 1 of this manual.

The Steri-Cult incubators can be equipped with a built-in Gas Guard system (P/N 1900153) that will operate with a CO

2 gas supply. The Gas

Guard uses two pressure switches to continuously monitor the pressures of two independent CO

2 supplies and automatically switches from one

supply to the other when the supply pressure drops below 10 psig (0.690 bar). The Gas Guard’s design does not facilitate use by multiple incubators.

The CO

2 gas supplies must be equipped with two-stage pressure regulators

with gauges. The high pressure gauge at the tank should have a 0-2000 psig range and the low pressure gauge should have a 0-30 psig range. The gas supply to the incubator must be maintained at 15 psig (1.034 bar), ±5 psig. Gas pressures below 15 psig cause nuisance alarms to occur on incubators equipped with the built-in Gas Guard.

The CO

2 inlets for the Gas Guard are located

on the back of the cabinet. Using 1/4” ID tubing, connect one of the CO

2 supply tanks

to the fitting labeled CO2 Inlet #1 Tank. Connect the second CO

2 supply tank to the

fitting labeled CO2 Inlet #2 Tank. Install 3/8” hose clamps to secure the 1/4” ID

Figure 9-6. Inlet Connections

tubing to the fittings on the cabinet back. (Refer to Figure 9-6.)

De-activating the Gas

Guard

Operation of the CO

Gas Guard

The built-in Gas Guard is turned ON when shipped from the factory. In addition, the Tank Select for the Gas Guard is specified as Tank 1 when shipped. Refer to Section 4, Configuration, to de-activate the Gas Guard, or change the Tank Select from #1 to #2. If the Gas Guard system is deactivated, the incubator functions normally by supplying CO

2 from the

supply tank connected to Inlet #1.

With the Gas Guard in operation, the incubator uses the gas supplied by the tank connected to Inlet #1 until the pressure drops below 10 psig (0.690 bars). At this time, the Gas Guard automatically switches to the gas supplied through CO2 Inlet #2.

7023307 Steri-Cult 9-5Thermo Scientific

Section 9

Factory Options

Operation of the CO2

Gas Guard (continued)

In addition, the incubator automatically changes the Tank Select in Configuration mode from 1 to 2 to indicate that the incubator is now using gas supplied through Inlet # 2. If the gas supply to Inlet #1 is replenished, the incubator will continue to operate using the gas supplied through Inlet #2 unless the operator changes the Tank Select from #2 to #1 through Configuration mode. Refer to Section 4, Configuration.

Audible and visual alarms occur on the control panel when the gas guard switches from one supply to the other. The audible alarm sounds until the operator presses the Silence key on the control panel. The visual alarm in the Message Center reads “Tank 1 Low” while the audible alarm is sounding, but the message is removed when the operator presses the Silence key. However, the ‘Tank Low’ indicator on the control panel stays lighted until the condition is resolved. The unit

Figure 9-7. Tank Low Indicator

operates normally.

Caution Both the audible and visual alarms described above do not ring back after the Silence key is pressed.

Dataloggger

If the Gas Guard system does not detect an adequate gas supply at CO2 Inlet #1 or Inlet #2, a visual and audible alarm again occurs on the control panel. The visual alarm in the Message Center reads “Tank 1&2 Low”. The audible alarm continues to ring until the Silence key is pressed. The audible alarm rings back every 15 minutes after the alarm is silenced if the Gas Guard continues to detect that both gas supply pressures are below 10 psig (0.690 bars).

1. To install the datalogger sensor (P/N 201912), locate the sensor and cable in the bottom of the incubator chamber.

2. Insert the grommet provided into the only round hole in the side of the diffuser pan.

3. Then route the sensor through this grommet, from the bottom of the diffuser pan.

4. Place the datalogger sensor in the desired location or use the clip provided. See Figure 9-8 (clip shown on pilaster).

9-6 7023307 Steri-Cult Thermo Scientific

5. See the ELPRO operating manual for settings.

Figure 9-8. Sensor

Section 10 Specifications*

Temperature

Control . . . . . . . . . . . . . . . . . . . .±0.1°C @ 37.0°C

Setpoint . . . . . .Digital touch pad, 0.1°C resolution

Range5°C over ambient to 50°C, factory default 10.0°C (disabled)

Uniformity±0.2°C @ +37°C, in a 23°C ambient @ nominal line voltage

Display . . . . . . . . .Digital, LED, 0.1°C increments

Sensor . . . . . . . . . . . . . . . . . . . . . . . . . .Thermistor

Temperature Safety

Setpoint . . . . . . . . . . . . . . .Fixed @ 160°C, ±5.6°C

Sensor . . . . . . . . . . . . . . .Thermostat, manual reset

ActionRemoves voltage from heaters to disable heat control

Relative Humidity

Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . .±2.0%

Range . . . . . . . . . . . . . . . .Ambient level to 90%**

Controller . . . . . . . . . . . . . . . . . . . .Microprocessor

Humidity system . . . . . . . . . . . . .95% RH at 37°C

Setpoint . . . . . . . .Digital touch pad, 1% resolution

Display . . . . . . . . . . . .Digital LED, 1% resolution

Control . . . . . . . . . . . . . . . . . . . . .±0.1% @ 5.0%

Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Infrared

Setpoint . . . . . .Digital touch pad, 0.1°C resolution

Range . . . .0 to 20%, factory default 0% (disabled)

Inlet Pressure . . . . . . . . . . . . . . .15 PSIG, ±5 PSIG

Controller . . . . . . . . . . . . . . . . . . . .Microprocessor

Display . . . . . . . . . .Digital LED, 0.1% increments

*Specifications are based on nominal voltages of 115V or 230V in ambients of 22°C

to 25°C.

**Humidity greater than or equal to 90% may lead to condensation depending on

environmental conditions in the work area.

7023307 Steri-Cult 10-1Thermo Scientific

Section 10

Specifications

Shelves

Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

Maximum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

Construction . . . . . . . . . .Stainless steel, perforated

Clearance . . . . . . . . .Adjustable on 1.160” centers

Dimensions . . (3307/3308)17.7” x 19.9” (45.2cm x 50.5cm)

Surface area . . . . . . .2.4 sq. ft. per shelf

Dimensions . . (3310/3311)25.7” x 19.9” (65.5cm x 50.5cm)

Surface area . . . . . . .3.6 sq. ft. per shelf

Loading . . . . . . . . . . . . .50 lbs. (22.7kg) stationary

Sterilization Cycle

Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . .140°C

Cycle time . . Approximately 14 hours @ 23°C, nominal line voltage

Activation . . . . . . . . . . . . . . . . . .Digital touch pad

Construction

Interior volume

3307/3308 . . . . . . . . . . . .8.2 cu. ft. (232.2 liters)

3310/3311 . . . . . . . . . . .11.4 cu. ft. (322.8 liters)

Interior . . Type 304 stainless steel mirror finish, coved corners

Exterior . .18 gauge cold rolled steel, powder-coated

Outer door gasket . . Four-sided, molded, magnetic vinyl

Inner door gasket . . . . . . . . . . . . . .Silicone feather

Insulation . . . . . . . . . . . . . . . . . . . . . .Mineral wool

Fittings

Access port1.4” (3.6cm) with removable silicone plug

CO2 inlet . . . . . . . . . . .1/4” (0.64cm) hose barbed

Drain port . . . 3/8” (0.95cm) barbed fitting with shutoff

10-2 7023307 Steri-Cult Thermo Scientific

Section 10

Specifications

Electrical

115V models . . . 115VAC, 50/60 Hz, 1PH (Operating range 90125VAC), Model 3307 10.5 FLA, Model 3310 11.5 FLA

Circuit breaker power switch . . 15A, 2 Pole (Model 3307/3310)

230V models . . . 230VAC, 50/60 Hz, 1 PH (Operating Range 180250VAC), Model 3308 5.4 FLA, Model 3311 5.9 FLA

Circuit breaker power switch . . 10A, 2 Pole (Model 3308/3311)

Power interconnect . . . . . . . . . . . . .IEC 320, male

Power inlet cord . . . 8’ (2.4m) cord with IEC 320 female plug and

country specific plug

Accessory outlet . . Standard, 115V/220V export, 75 watts max. (one per

chamber)

Alarm contacts . . .Deviation of temperature, CO2, RH, power failure.

N/O and N/C

Dimensions

Exterior . . (3307/3308)35.0” W x 39.4” H x 27.0” F-B (88.9cm x

100.1cm x 68.6cm)

(3310/3311)43.0” W x 39.4” H x 27.0” F-B (109.2cm x

100.1cm x 68.6cm)

Weight

Net weight . . . Model 3307/3308 - 330 lbs. (149.7kg),

Model 3310/3311 - 410 lbs. (186.0kg)

Shipping (motor) . . .Model 3307/3308 - 400 lbs. (181.4kg),

Model 3310/3311 - 480 lbs. (217.7kg)

Certifications

Refer to the Declarations of Conformity at the end of this manual

7023307 Steri-Cult 10-3Thermo Scientific

Section 10

Specifications

Safety Specifications

Indoor Use Only

Altitude . . . . . . . . . . . . . . . . . . Up to 2,000 meters

Temperature . . . . . . . . . . . . . . . . . . . .5°C to 40°C

Humidity . . . Maximum 80% RH for temperatures up to 31°C, decreasing linearly to 50% RH at 40°C

Mains Supply Fluctuations . . Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage

Installation Category II

Pollution Degree 2

Class of Equipment I

________________________________________________

Installation Category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply.

Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.

10-4 7023307 Steri-Cult Thermo Scientific

Section 11 Servicing

Service and maintenance information follow.

Parts List

Part Number Description

2270102

3310902 Pilaster, single

3310903 Shelf channel, right or left

1900446 Pilaster, 4 pack

1900447 Shelf channel, 10 pack

3310904 Diffuser pan, large (3310/3311)

3307904 Diffuser pan, small (3307/3308)

191682 Microboard, main control

230201 Fuse, 750mA, TD, 5 x 20mm (humidity system, 250V)

230135 Fuse, 1A, TD, 5 x 20mm (accessory outlet, 115V)

230120 Fuse, 0.5A, TD, 5 x 20mm (accessory outlet , 230V)

156125 Motor, hot air, 115VAC, 50/60 Hz

191681 Display board

290185 Probe, 2252 Ohm/25°C, 0.156 x 2

Preventive maintenance kit (door gasket, HEPA filter, CO2 air filter,

RH bacterial filter, access port filter, water bottle air filter)

1900160 HEPA filter kit (includes 760207 & 1900185)

224310 Stainless steel shelf, large

224307 Stainless steel shelf, small

231204 Infrared type CO2 sensor

250087 CO2 valve kit with assorted fittings

290174 RH sensor assembly, 1ft.

230180 12A, DPDT circuit breaker switch (power)

400201 Switcher kit, 40W

360213 Pressure switch (Gas Guard option)

250121 Solenoid gas valve (12VDC Gas Guard option)

7023307 Steri-Cult 11-1Thermo Scientific

Section 11

Servicing

Parts List

(continued)

Part Number Description

113008 Leveling foot

1900161 VOC HEPA filter kit (includes 760208 & 1900185)

250049 12V valve (water fill)

250128 12V valve (RH air valve)

360251 Door switch, outer

360263 Door switch, inner

280096 Blue LED light source

100119 3.5 x 1.5 CCW blower wheel

139033 Humidifier, humidity system

360171 Liquid level switch (float)

360255 Liquid level switch (protection float)

900140 1.6” sq. 12VDC fan x CFM

130625 Tubeaxial fan 31 CFM 115V Fan

105070 Inner glass door

247013 Humidity system drain fitting

760208 HEPA/VOC filter only

1900199 Complete blower assembly (includes motor, wheel, scroll, mounting materials)

1900200 Complete humidifier assembly (includes nebulizer and housing)

1900201 RH conditioner assembly, 115V

1900202 RH conditioner assembly, 230V

1900233 Temperature sensor assembly (290185 sensor)

50109579 Water cleaning cell (for water reservoirs, pans, and bottles)

130101 Water cleaning cell (quantity of 6)

11-2 7023307 Steri-Cult Thermo Scientific

Section 11

Servicing

Replacing the Fuses

Note See Wiring Diagram 3307-71, page 1 of 2, Fuse Holder Detail 2. s

Warning The following procedure requires accessing the electronics area.

Qualified personnel only should perform this procedure. Wearing a

electrostatic discharge wrist band or other grounding device is

recommended.

To access the only replaceable fuse in the incubator, follow the steps below.

1. Turn off the incubator’s power switch and disconnect the power cord.

2. Remove the four screws from the left side panel. Set the panel aside.

3. On the left side of the opening is the electronics area. Locate the fuse. See Table 11-1 below for replacement fuse specifications. Replace the blown fuse with a new one.

4. Install the panel and secure with the screws.

5. Return the unit to service. If the fuse blows after restoring power to the incubator, contact the Technical Services Department.

Table 11-1. Fuse Specifications

Fuse Voltage Manufacturers Part # Amperage Rating Rupture Speed IEC Letter Code

115VAC ACC BUSS GMC-1.0A 1.0 Amp Time-Lag T

250VAC ACC BUSS GMC-740mA 750mAmp Time-Lag T

230VAC ACC BUSS GMC-500mA 0.5 Amp Time-Lag T

Replacing the CO2

and RH Air Filters

Warning The following procedure requires accessing the electronics area.

Qualified personnel only should perform this procedure, using preventive maintenance kit # 2270102. Wearing a electrostatic discharge wrist band or other grounding device is recommended.

The CO2 and RH air filters are recommended to be replaced annually. Follow the steps below to replace the filters.

1. Turn off the incubator’s power switch and disconnect the power cord.

2. Remove the four screws from the left side panel. Set the panel aside.

7023307 Steri-Cult 11-3Thermo Scientific

Section 11

Servicing

Replacing CO2 & RH

Air Filters (continued)

3. On the left side of the opening is the electronics area. Locate the CO2 air filter (Figure 11-1). Disconnect carefully from the tubing and replace.

Figure 11-1. CO2 Air Filter

Figure 11-2. RH Air Filter

4. In the right side of the opening is the humidity system. Locate the RH air filter in the upper right corner (Figure 11-2). Disconnect from the tubing and replace.

5. Install the panel and secure with the screws.

6. Return the unit to service.

11-4 7023307 Steri-Cult Thermo Scientific

Section 11

Servicing

7023307 Steri-Cult 11-5Thermo Scientific

11-6 7023307 Steri-Cult Thermo Scientific

Section 11

Servicing

Section 11

Servicing

7023307 Steri-Cult 11-7Thermo Scientific

7023307 Steri-Cult 11-8Thermo Scientific

Section 11

Servicing

11-9 7023307 Steri-Cult Thermo Scientific

Section 11

Servicing

Section 11

Servicing

7023307 Steri-Cult 11-10Thermo Scientific

Section 11

Servicing

11-11 7023307 Steri-Cult Thermo Scientific

Section 11

Servicing

7023307 Steri-Cult 11-12Thermo Scientific

Section 11

Servicing

7023307 Steri-Cult 11-13Thermo Scientific

Section 11

Servicing

11-14 7023307 Steri-Cult Thermo Scientific

Section 11

Servicing

7023307 Steri-Cult 11-15Thermo Scientific

2 sensor (IR) will be warranted

ISO

9001

REGISTERED

Rev. 3 4/09

THERMO FISHER SCIENTIFIC STERI CULT INCUBATOR WARRANTY USA*

The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows shipping time so the

warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any

subsequent owner during the first year warranty period.

During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at

Thermo's expense, including labor. In addition, after the initial warranty period of one year, the CO

for a second year, parts only. The blower motor will be warranted for three years, total, parts only. Installation and calibration are

not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and

direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.

Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment

or to the component part beyond the original warranty period. The Technical Services Department must give prior approval for

return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to Thermo postage paid

and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED.

NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo Fisher

Scientific shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits

or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives.

Printed instruction manuals carefully detail equipment installation, operation and preventative maintenance.

If equipment service is required, please call your Technical Services Department at l-800-438-4851 (USA and Canada) or 1-740-

373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special applica-

tions. Outside the USA, contact your local distributor for warranty information.

THERMO FISHER SCIENTIFIC INTERNATIONAL STERI CULT INCUBATOR WARRANTY*

The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows shipping time so the

warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any

subsequent owner during the first year warranty period.

During the first year component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at

Thermo's expense, excepting labor. In addition, after the initial warranty period of one year, the CO

sensor (IR) will be warranted

for a second year, parts only. The blower motor will be warranted for three years, total, parts only. Installation and calibration is not

covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direc-

tion prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.

Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment

or to the component part beyond the original warranty period. The Technical Services Department must give prior approval for

return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to Thermo postage paid

and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED.

NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be

liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products.

Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives.

Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.

If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or 1-740-

373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special application.

Outside the USA, contact your local distributor for warranty information.

ISO

9001

REGISTERED

Rev. 3 4/09

H7 TURCK Inc. 3000 Campus Drive Minneapolis, MN 55441 Application Support: 1-800-544-PROX Fax: (763) 553-0708 www.turck.com

UNLOCK

OCK

picofast “Snap Lock” Connector Instructions

Installation Instructions

To Attach:

1. Be sure black locking sleeve is pulled back.

2. Line up pins and push connector onto plug. You will feel a “snap.” Do not twist.

3. Push locking sleeve forward on connector until it is flush with the front of the connector.

4. If sleeve is difficult to slide

on a new connector, “e x e r c i s e ” i t a f e w t i m e s . Do not use tools.

With TURCK’s patented Locking Sleeve pulled back, any picofast sensor slides on without any difficulty.

After sensor is connected, push Locking Sleeve for

ward to create a watertight connection. It’s a snap!

1. Do not twist.

2. Pull locking sleeve back to “unlocked” position.

3. Pull connector

straight off.

4. Leave locking sleeve in

unlocked position.

To Detach:

Thermo Fisher Scientific 401 Millcreek Road Marietta, Ohio 45750 United States

www.thermofisher.com

Steri-Cult 3307 Series, 3310 Series Operating And Maintenance Manual

Specifications and Main Features

Frequently Asked Questions

User Manual