Statebourne cryogenics Biosystem Access, Biosystem Archive, Biosystem 12, Biosystem 24, Biosystem 50 User Manual

User Manual

Biosystem Series

Tel: [phone] Fax: [phone] Email: [e-mail]

Biosystem Access Series Biosystem 12
Biosystem 24
Biosystem 50

Biosystem Archive Series Biosystem 36
Biosystem 40
Biosystem 45
Biosystem 55

Biosystem 60
Biosystem 80
Biosystem 100
Biosystem 100 Plus

18 Parsons Road,
Parsons Industrial Estate,
Washington, Tyne and Wear
NE37 1EZ

STA TEBOURNE

cryogenics

Electronic Set-up and Installation

CONTENTS

SAFETY 4

INTRODUCTION 6

CONNECTION TO LIQUID NITROGEN SUPPLY 7

INITIAL FILL WHEN USING LIQUID PHASE SETTINGS 7

DESCRIPTION OF LEVEL CONTROLLER COMPONENTS 8

CONTROLLER INSTALLATION 10

INITIAL SETTING UP 10

SYSTEM SETUP PROCEDURE 11

CLOCK SETTINGS - READJUSTMENT 17

NORMAL OPERATION 17

ALARM REPORTING 17

TESTING 18

SYSTEM FAULTS 19

DATA LOGGING 20

PRINTING THE DATA LOG 20

CLEARING THE DATA LOG 23

DATA RECOVERY 24

DOWNLOADING DATA TO A PC 24

TEMPERATURE RECORDER OUTPUT 24

SIMULTANEOUS FILLING 25

REMOTE ALARM CONNECTION 25

AUXILIARY RELAY 25

24V AC OUTPUT 25

Page 2

Appendix A 26
SENSOR POSITIONING

Appendix B 28
OPTION SWITCH SETTINGS

Appendix C 31
CONNECTION TO A PRINTER/PC

Appendix D 32
CALIBRATION OF TEMPERATURE READOUT

Appendix E 33
SIMULTANEOUS FILL CONNECTION

Appendix F 34
ALARM CODES/TROUBLESHOOTING

Appendix G 35
MAINTENANCE

Appendix H 36
REPLACEMENT PARTS

MANUAL REVISION INDEX AND APPROVAL

37

Page 3

SAFETY

Introduction

In all cryogen handling the following appropriate safety guidelines are essential if damage to equipment and
personal injury are to be avoided. The following guidelines provide a basic level of instruction in the precautions
necessary but are by no means exhaustive, a comprehensive approach involving staff training on a recognised
course is strongly recommended.

Liquid Nitrogen

The boiling point of liquid nitrogen is -196°c (77K).
The molecular weight of nitrogen is 28.
Nitrogen gas is inert, odourless, tasteless and colourless.
Nitrogen gas is an asphyxiant.
Liquid nitrogen is odourless, colourless, slightly lighter than water and does not produce toxic or irritating
vapours.

Hazards and Precautions

Cryogenic Burns
Appropriate protective clothing should always be worn when handling cryogenic equipment.
The extreme cold can cause cryogenic burns which have a similar effect on the skin to frostbite.
Similar methods should be used in the treatment of both.
Re-warm the affected area as quickly as possible in WARM water at about 40°c or by warm air.
Maintain general body warmth.
Do not rub or massage the affected area as this may cause more serious damage than already exists.
Seek medical advice as soon as possible.

Oxygen Depletion
Liquid nitrogen should always be stored and used in well ventilated areas and the use of oxygen deficiency
monitors is strongly recommended.
Liquid nitrogen expands when vaporised into a gaseous state by a factor of 842 times, causing a significant
danger from asphyxiation in confined or poorly ventilated spaces. Suffocation can occur rapidly as the oxygen is
displaced. (A drop of only 2% in the oxygen content is enough to pose a significant risk.)
Loss of consciousness or dizziness while working with liquid nitrogen are obvious signs of asphyxiation due to
an oxygen deprived atmosphere. At the first signs of this the subject should be moved as quickly as possible
from the affected area. If breathing has stopped apply artificial respiration and summon medical assistance
immediately.

Condensed Air
Due to extreme cold, air on the outside of transfer hoses and dewars can condense and form liquid air.
Adequate ventilation should ensure that any risk from this occurring is minimised. However, as with liquid
oxygen a strict no smoking or naked flame rule must be observed.

Page 4

Protective Equipment

Cryogloves
The wearing of a suitable pair of gloves is essential for day to day operations with liquefied gases.
Gloves generally fall into two categories. Those for handling objects in cold gas vapours are usually gauntlets of
mid-arm or shoulder length. For handling liquid dispensing hoses and tanks, heavy duty leather wrist length
gloves can be used.

Face Shields
Clear visors are available to protect eyes and face from liquid splashes.

Aprons
Thermal aprons can also be worn to protect against liquid splashes.

Footwear
Appropriate safety footwear should be worn at all times. Lightweight footwear and open sandals are not
recommended for obvious reasons. Wellington or loose fitting boots should be tied at the top to ensure that no
liquid can enter. For best protection use only approved safety footwear.

Oxygen Deficiency Monitors
Analysis of the oxygen content in an atmosphere is often overlooked but can be one of the most fundamental
safety measures. A monitor can provide a constant analysis and thus increase safety.

All of the above safety equipment is available from Statebourne’s Customer Service Department who
will be pleased to help with any queries you might have.

Page 5

INTRODUCTION

All Biosystem Series refrigerators utilise the M265CE microprocessor based liquid nitrogen level controller.

The M265CE will perform the following functions.

*Level monitoring and automatic filling of refrigerators.

*Level display (High/Normal/Low).

Temperature monitoring and display.

Generation of local and remote alarms for:

High Temperature
Underfilled
Overfilled
Sensor faults
Failure of liquid nitrogen supply
Lid left open.

*Manual filling.

*Chart recorder output.

*Logging of temperature, levels, alarms, fill activity etc.

*Serial port for printer output of logged data.

*Self testing and isolation of sensor faults.

*Lid open detection.

*Simple “daisy chaining” of controllers for simultaneous filling in multiple installations.

*Automatic defogging on lid opening. n

*Fast temperature recovery on lid closure. (‘Quick Chill’). n

*Communication with PC for downloading of data.

n These functions will be disabled if a fill delay switch is fitted.

Fill delay switch will only be fitted if specifically requested where, for example, automatic venting of the
nitrogen supply pipework is incorporated to allow for gas produced during cool down to be prevented from
blowing through the Biosystem.

Page 6

CONNECTION TO LIQUID NITROGEN SUPPLY

The Biosystem Series refrigerators should be supplied with liquid nitrogen ONLY, from a liquid cylinder or bulk
tank operating at between 10psiG and 20psiG maximum.
Operating pressures in excess of this will increase transfer losses and could cause the liquid level in the sensor
tube to fluctuate and send false signals to the controller, thus causing the refrigerator to underfill.
If the supply pressure were to exceed the lifting pressure of the Biosystem’s own relief devices the entire
contents of the supply vessel could be discharged, causing a hazard in the area of the refrigerator and
dramatically increasing operating costs.

The liquid fill connection on the refrigerator is ½”BSPP female and incorporates a filter and in-line relief valve
which should not be removed or by-passed while in service.

Before connecting to the Biosystem refrigerator the supply hose and, where applicable the super insulated
vacuum line should be thoroughly purged with warm nitrogen gas to remove any moisture which may
contaminate the seats of the solenoid valves or sensor probes with ice particles. Connections should be free
from grease and dirt and checked for leaks.

To minimise transfer losses, supply lines should be kept as short as possible and insulated with a suitable
material.

A shut off valve from the supply vessel should be located close to the Biosystem refrigerator for use in
emergencies and for maintenance purposes. Care should be taken to include a suitable pressure relief device
between any two shut off valves as any liquid trapped in the line will expand by more than 800 times as it
warms up and could burst the pipe or fracture one of the couplings.

INITIAL FILL

Important:-

The initial fill must be carried out with the lid open in order to avoid possible permanent

damage to the lid assembly.

When filling a warm vessel to operate in liquid phase, the fill should be carried out in stages due to the massive
amounts of cold gas formed which could cause ice build ups around the venting area at the rear of the vessel
which is in the proximity of the thermocouple. Ice build up in this area could cause damage to the thermocouple
when the lid is opened and so is to be avoided.

The initial liquid phase fill on a Biosystem 24 for example will take about 3 hours if allowed to fill continuously. It
is strongly recommended that the refrigerator is allowed to fill in cycles of about one hour with intervals of one
hour in between to allow the vessel to settle.

Page 7

DESCRIPTION OF LEVEL CONTROLLER COMPONENTS

The M265CE System consists of the following items:-

Controller (M266CE) _ Part Number 8601044

Connector PCB + Cable Assembly (M267CE) _ Part Number 8601045

Sensor Assembly with Thermocouple (M268CE) _ Part Number 8601046

Safety Transformer (M269CE) _ Part Number 8601973

M265CE Controller

The Control panel features four function keys, Alarm, Level and Fill LEDs, and a 3 digit LED display. (Fig 1.).
The digital display indicates the temperature within the refrigerator and in conjunction with the function keys
may be used to read and alter the systems settings.
In Alarm conditions the Alarm LEDs indicate the nature of the fault. The digital display gives additional
information by means of alarm codes.
Level is indicated by 3 LEDs as High, Low or Normal and at the extremes by means of Overfill and Low level
alarm LEDs.
A Fill LED indicates when the refrigerator is being filled.

M267CE Connector PCB

This provides two part screw terminal connectors for all external cables. A single, plug in 20 way ribbon cable
links the controller to the connector PCB.
This arrangement allows the gland plate and external cables to be connected to, or removed from the
refrigerator without disturbing any permanent connections.

M268 Sensor Assembly

This is the 4 level Thermistor Sensor Assembly with Thermocouple.

M269CE Safety Transformer

This supplies power at 24V AC to operate the controller.

Page 8

Page 9

CONTROLLER INSTALLATION

The Controller will normally be installed by the manufacturer of the Refrigerator.

If installing the Controller check that the option switches are correctly set for the installation. These are located
just behind the display, on the Controller PCB.
Details of the options and switch settings are given in Appendix B.

INITIAL SETTING UP

If a printer is to be used set it up and connect to the 9 way D type connector on the gland plate at the rear of the
refrigerator and change S5 to the off position (see Appendix B). For cable details and printer settings see
Appendix C.

Switch on the printer.

Printer connection

The sensor positions are pre-set in the factory to suit Statebourne’s inventory control systems (vapour phase or
liquid phase depending on order specification). See Appendix A for details.

Plug the M269CE Safety Transformer into the mains supply and switch on.

The audible alarm may sound even if the refrigerator has been filled. Use the Mute switch to silence the
audible alarm.

If the refrigerator is empty or the LN2 level is low the refrigerator will begin to fill. Further alarms may occur and
these may be silenced using the Mute switch. Ignore any alarm messages for the time being.

When the refrigerator has finished filling the controller must be initialised.

To do this switch off the power to the refrigerator, then switch on the power whilst pressing and holding the
secret key. The alarm will sound three times and the following message will appear.

Release the switch. If a printer is connected it should record the time power was restored and a System Reset.

Page 10

After a couple of seconds the digital display will show the temperature and the Level LEDs will show the Level.

The temperature readout is calibrated at the factory and should be correct. If not, it may be re-calibrated as per
Appendix D.

After initialisation the System Set-up should be carried out.

Two set-up modes are possible:

a) Defaults are Enabled by means of the DIL switch. Only the alarm temperature, refrigerator no. and

time/date need to be entered.

b) Defaults are not Enabled. All alterable parameters must be entered.

Note that the following description of the system set-up procedure assumes that the default settings are not
enabled.

SYSTEMS SETUP PROCEDURE

To enter the set-up routine press the Mute and Test keys simultaneously.

The controller will bleep twice and the Version No. of the Controller firmware will be displayed.

Temperature Alarm

After a short time the Temperature LED will light and a temperature will be displayed on the digital display.
This is the alarm temperature.

Use the TEST key to decrease the alarm temperature.
Use the MUTE key to increase the temperature.

The Alarm Temperature is adjustable in 5 degree steps between 0 and -150 C.

If the temperature of the Thermocouple rises above this pre-set the value an alarm condition will occur.

Press the FILL key when finished.

Page 11

Fill Timer

The LN2 SUPPLY LED will light and a number will be displayed. This is the Fill Timer setting in minutes.

Use the TEST key to increase the Fill Timer setting.
Use the MUTE key to decrease the Fill Timer setting.

The Fill Time is adjustable in 5 minute steps between 0 and 125 minutes.

If the solenoid valve is operated for longer than this pre-set time an alarm condition will occur.

Press the FILL key when finished.

Remote Alarm Timer

The REMOTE LED will light and a number will be displayed.
This is the Remote Alarm Timer setting in minutes.

Use the TEST key to increase the Remote Alarm Timer setting.
Use the MUTE key to decrease the Remote Alarm Timer setting.

The Remote Alarm Timer is adjustable in 5 minute steps between 0 and 60 minutes.

The Remote Alarm Timer sets the time which must elapse between the first alarm condition and activation of
the remote alarm relay. The exception to this is the Overfill alarm which defaults to 5 minutes irrespective of the
Remote Timer setting.

Press the FILL key when finished.

Lid Timer

The LID LED will light and a number will be displayed. This is the Lid Timer setting in minutes.

Use the TEST key to increase the Lid Timer setting.
Use the MUTE key to decrease the Lid Timer setting.

The Lid Timer is adjustable in 5 minute steps between 0 and 30 minutes.

Page 12