Sorvall WX 100 Ultra, WX 90 Ultra, WX 80 Ultra Instruction Manual

50105750-4 October 2010

Thermo Fisher Scientific

WX Ultra Series
WX 100, WX 90, WX 80

Instruction Manual

© 2010 Thermo Fisher Scientific Inc. All rights reserved.

WX

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The contents of this document are subject to change without notice. All technical information in this
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all previous information received by the purchaser.

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Release history: 50105750-4 printed in October 2010.

For Research Use Only. Not for use in diagnostic procedures.

Thermo Scientific WX Ultra Series i

C

Chapter 0 Preface - Intended use and safety definitions ...............................................................................................iii

Safety Definitions..................................................................................................................................iii

Important safety reminder .....................................................................................................................iv

Chapter 1 Description...........................................................................................................................................................1-1

General description............................................................................................................................. 1-1

External view of ultracentrifuge ...................................................................................................... 1-2

Design................................................................................................................................................ 1-3

Control panel ................................................................................................................................. 1-3

Rotor chamber................................................................................................................................ 1-6

Safety devices.................................................................................................................................. 1-7

Rotor adapter ................................................................................................................................. 1-8

Chapter 2 Operation..............................................................................................................................................................2-1

Run preparation ................................................................................................................................. 2-2

Starting up the ultracentrifuge ........................................................................................................ 2-3

Preparing tubes/bottles and rotor.................................................................................................... 2-4

Basic operation ................................................................................................................................... 2-4

Setting run conditions .................................................................................................................... 2-4

Setting user ID code ....................................................................................................................... 2-9

Using Rotor Life Management .....................................................................................................2-10

Basic operating procedure................................................................................................................. 2-11

Normal operation......................................................................................................................... 2-13

Zonal operation............................................................................................................................ 2-15

Acceleration and deceleration rates ................................................................................................... 2-17

How to use the FUNCTION field...................................................................................................2-19

Programmed operation ................................................................................................................. 2-21

Step-mode operation .................................................................................................................... 2-32

RTC (real-time control) feature.................................................................................................... 2-40

Displaying and setting RCF.......................................................................................................... 2-48

Setting w2T.................................................................................................................................. 2-56

Defrost function ........................................................................................................................... 2-58

Setting and resetting zonal operation ............................................................................................ 2-59

Rotor management........................................................................................................................... 2-60

Rotor life management ................................................................................................................. 2-60

Automatic rotor deration.............................................................................................................. 2-61

Automatic rotor exclusion............................................................................................................. 2-61

Registering a rotor ........................................................................................................................ 2-61

Deleting a registered rotor ............................................................................................................ 2-64

Updating the existing data for a rotor ........................................................................................... 2-65

When power failure occurs ............................................................................................................... 2-66

Features of the menu screen.............................................................................................................. 2-69

Contents

Contents

ii WX Ultra Series Thermo Scientific

Centrifuge scheduler..................................................................................................................... 2-69

User list ........................................................................................................................................ 2-71

Alarm information........................................................................................................................ 2-76

Rotor catalog ................................................................................................................................ 2-77

User Customizations..................................................................................................................... 2-77

Print utilities (option)....................................................................................................................... 2-81

Operating procedure..................................................................................................................... 2-82

Contents of print utilities ............................................................................................................. 2-84

Automatic run result printing....................................................................................................... 2-86

Printer fault handling ................................................................................................................... 2-87

Lockout (optional)............................................................................................................................ 2-88

Chapter 3 Maintenance........................................................................................................................................................3-1

Rotor chamber.................................................................................................................................... 3-1

Drive spindle ...................................................................................................................................... 3-2

Cabinet............................................................................................................................................... 3-2

Replacement parts .............................................................................................................................. 3-2

Service decontamination policy .......................................................................................................... 3-2

Chapter 4 Troubleshooting ..................................................................................................................................................4-1

Alarm indicators ................................................................................................................................ 4-1

Diagnosed problems - requiring maintenance..................................................................................... 4-3

Non-diagnosed problems ................................................................................................................... 4-3

Chapter 5 Preinstallation.....................................................................................................................................................5-1

Location Requirements....................................................................................................................... 5-1

Electrical requirements ....................................................................................................................... 5-2

Leveling.............................................................................................................................................. 5-2

Moving the ultracentrifuge ................................................................................................................. 5-3

Chapter 6 Specifications .....................................................................................................................................................6-1

Chapter 7 Supply list.............................................................................................................................................................7-1

Appendix A Service Decontamination Policy....................................................................................................................A-1

Appendix B Condensed operating instructions .................................................................................................................B-1

Appendix C Chemical Compatibility Chart .........................................................................................................................C-1

Appendix D WARRANTY.........................................................................................................................................................D-1

Appendix E Contact Information ...........................................................................................................................................E-1

Thermo Scientific WX Ultra Series iii

P

Preface - Intended use and safety definitions

This manual is a guide to the use of the Sorvall WX Ultra series ultracentrifuge.

Information herein has been verified and is believed adequate for the intended use of the centrifuge.
Because failure to follow the recommendations set forth in this manual could produce personal injury
or property damage, always follow the recommendations set forth herein. Thermo does not guarantee
results and assumes no obligation for the performance of centrifuges or other products that are not
used in accordance with the instructions provided. This publication is not a license to operate under,
nor a recommendation to infringe upon, any process patents.

Safety Definitions

DANGER, WARNING, CAUTION, and NOTE within the text of this manual are used to
emphasize

important and critical instructions.

DANGER, WARNING and CAUTION information is accompanied by a hazard symbol and appears
throughout the manual, both on the Important Safety Reminder pages and near the information it
corresponds to.

Before you operate the centrifuge, we recommend that you read this instruction manual thoroughly,
particularly all DANGERS, WARNINGS and CAUTIONS.
Never operate the centrifuge without first considering all items on the Important Safety Reminder
pages, and never operate the centrifuge in any manner not described in this instruction manual.

DANGER Informs the operator of an extreme hazard or an unsafe practice that will result
in death or serious injury.

WARNING Informs the operator of a hazard or an unsafe practice that could result in
serious injury or death, affect the operator's health, or contaminate the environment.

CAUTION Informs the operator of a hazard or an unsafe practice that could cause minor
injury, or result in damage of equipment or property.

Note Highlights essential information that is not hazard-related.

Preface - Intended use and safety definitions

Important safety reminder

iv WX Ultra Series Thermo Scientific

Important safety reminder

Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure safe
operation of this centrifuge, anyone using it should be aware of all safe practices and take all
precautions described below and throughout these operating instructions.

DANGER

• Before removing any cabinet panel (such as for maintenance or repair), always turn
the main Power Switch OFF and unplug the centrifuge, then wait at least three
minutes to eliminate the potential for severe electric shock.

WARNING

• Never exceed the maximum rated speed of the installed rotor; to do so can cause rotor
failure.

• Always reduce (derate) rotor speed whenever:

1. The rotor speed/temperature combination exceeds the solubility of the gradient
material and causes it to precipitate.

2. The compartment load exceeds the maximum allowable compartment load (or design
mass) specified for your rotor.

Failure to reduce speed under these conditions can cause rotor failure.

• Always inspect the rotor as specified in your rotor manual. Do not use a rotor that
shows signs of damage or corrosion.

• Centrifuges routinely deal with high energy levels and could move suddenly in the
unlikely event of rotor failure. During centrifuge operation, never lean on or move the
centrifuge, keep the surrounding area clear of objects (including all hazardous and
flammable materials), and do not work on top of or next to the centrifuge (as a rule,
keep people and objects at least 300 mm [12 inch] away).

• Never unlock the chamber door, attempt to open the door, or otherwise attempt to
touch the rotor while it is rotating. In the event of a power outage, it can take more
than three hours for the rotor to stop - be sure to wait at least three hours before
opening the door.

• Never attempt to override or otherwise disable any safety features.

• When using radioactive, toxic, or pathogenic materials, be aware of all characteristics
of the materials and the hazards associated with them in the event leakage occurs
during centrifugation. If leakage does occur, neither the centrifuge nor the rotor can
protect you from particles dispersed in the air. To protect yourself, we recommend
additional precautions be taken to prevent exposure to these materials, for example,
use of controlled ventilation or isolation areas.

• Always be aware of the possibility of contamination when using radioactive, toxic, or
pathogenic materials. Take all necessary precautions and use appropriate
decontamination procedures if exposure occurs.

Preface - Intended use and safety definitions

Important safety reminder

Thermo Scientific WX Ultra Series v

WARNING

• The use of sealed rotors, buckets and/or sample containers will provide increased
protection from contamination during routine operation. However, these items will
not guarantee contamination protection from accidents resulting in damage to the
rotor or buckets. Do not run hazardous materials in the centrifuge unless placed in a
biohazard enclosure and operated using all appropriate safety precautions.

• Never use any materials capable of producing flammable or explosive vapors, or
extreme exothermic reactions.

• The centrifuge is equipped with a three-wire power cord that has one wire for
connection to ground. The centrifuge must be correctly grounded to guard against
shock hazards.

CAUTION

• Do not operate the control keys using a ball-point pen or a sharp object.

• Do not run or precool the rotor at the critical speed as this will have a detrimental
effect on centrifuge component life (see rotor manual).

• Do not operate the centrifuge with a rotor that is not balanced within specification.
To do so can cause damage to the centrifuge drive assembly.

• Always make sure the rotor is correctly assembled and properly seated on the
centrifuge drive spindle before operation. If using a swinging-bucket rotor, always
make sure that a bucket is installed at each position and that each is properly seated
and can swing freely after installing the rotor on the centrifuge drive spindle (see rotor
manual).

• Do not exceed the maximum speed of the tubes, bottles, or adapters being used.
Check manufacturer's published specifications or see Sorvall Product Guide; if unsure,
to avoid loss of valuable sample, we recommend performing a test run.

• Do not continue to operate the centrifuge if abnormal sounds occur during operation.
Immediately discontinue use of the centrifuge and contact Thermo Service.

• Supply power must be checked before the centrifuge is connected to power because
the centrifuge can be damaged if connected to the wrong voltage. Thermo is not
responsible for incorrect installation and warranty is void if an initial installation or
electrical modification of the centrifuge is not performed by Thermo or a Thermo
representative.

• Before moving the centrifuge, always remove the rotor from the rotor chamber to
avoid damage to the drive assembly.

• Do not place fluids inside, on top of, or close to the centrifuge --- spillage can result in
electrical or mechanical failure.

• Always operate and maintain the centrifuge and all rotors as instructed in this manual
and in the rotor manual(s).

Preface - Intended use and safety definitions

Important safety reminder

vi WX Ultra Series Thermo Scientific

Thermo Scientific WX Ultra Series 1-1

1

Description

General description

The Sorvall WX Ultra series ultracentrifuges are designed and manufactured based on our long
experience in the development of centrifuges; they are easy to use and highly reliable. Features include
the following:

1. Maximum speed of 100,000 rpm (802,006 x g)

2. Control panel is simple with easy key operation and easy-to-see liquid crystal screen.

3. The displayed language can be switched over between English and Japanese.

4. The real-time control (RTC) feature enables setting a start time or a finish time, thus letting you
run your machine at a desired date and time.

5. Centrifugal force (RCFmax and RCFavg) can be displayed and set.

6. Twenty varieties of nine stepped modes can be programmed for a wide range of applications.

7. Various alarms notify users of causes and necessary actions when fault conditions are detected
allowing easier and quicker troubleshooting.

8.

Space saving design. The installation area required is 0.81 m2 (90 x 90 cm) pr 9.0 ft2 (3.0 x 3.0 ft). Lower

top deck makes it easy to install and remove the rotor.

9. Low noise level makes the ultracentrifuge suitable for use in any laboratory.

10. Samples can be easily balanced visually.

11. CFC-free thermoelectric cooling system with greater cooling capacity.

Advanced features (options)

The options of the Sorvall WX Ultra series ultracentrifuges are: Compass Software, Lockout Kit, and a
Printer.

The Compass Software is a calculation/simulation system for centrifuge support that is compatible

with

Windows

R 2000 / XP Professional. Compass Centrifugation software helps determine appropriate run

conditions prior to starting centrifugal separation, and calculates optimum centrifuge and rotor run
conditions for materials in which proper run conditions are unknown. For more information on this
please consult the Compass Operator's Manual.

The Lockout Kit limits centrifuge access to a group of registered users (up to 40) which is controlled by
lab managers/supervisors (up to 3). For more information on this see page 2-95, Lockout.

The printer provides hard copy reports of information maintained in the centrifuge including: Set and
actual run conditions, rotor use, programmed memory contents, and others. For more

information on

this see page 2 - 89, Print Utilities.

Description

General description

1-2 WX Ultra Series Thermo Scientific

External view of ultracentrifuge

The WX Series ultracentrifuge have the same external view, except for the model name printed on front
cover. The following is the external view of the WX ultracentrifuge.

External view of ultracentrifuge

Figure 1-1

External view of the Sorvall WX Ultra series

Note * This height is measured from level floor surface.

Top d eck

Door

Safety cover

Display panel

690 mm (27.2 in)
790 mm (31.1in)
850 mm (33.5 in)
1000 mm (39.4 in)

Operation key

Rotor rubber mat

Power switch

Front cover

Description

Design

Thermo Scientific WX Ultra Series 1-3

Design

Control panel

The control panel for the Sorvall WX Ultra series consists of a color screen, a touch sensitive display
panel and a keyboard.

The display panel incorporates an easy-to-read liquid crystal display. The display panel (field display)
displays running conditions and running status (this screen is called the Run Screen), along with
features such as Programmed Run, Rotors List, and User Customization Screens. Fig. 1-2 shows the
display panel, and Fig.1-3 represents the keyboard.

Figure 1-2 Display panel

Figure 1-3 Keyboard

(3) Message indicator

{

(1) Field display

(5) Vacuum indicator

(4) RUN mode indicator

(2) FUNCTION field

}

}

Operation state display field

Setting value display field

(12) Key pad

(11) Cursor key

(6) START key

(7) STOP key

(8) VACUUM key

(10) MENU key

(9) ESC key

Description

Design

1-4 WX Ultra Series Thermo Scientific

Table 1-1 [Functions of the display panel-keyed by item no. to fig. 1-2]

No. Name and Symbol Functions and actions

(1) Field display

SPEED

TIME

TEMP

Displays various fields.
The SPEED, TIME, and TEMP fields give the current status indicator in the top row and the
setting indicator in the bottom row. (For setting, see Section “Setting run conditions”.)

• SPEED (Speed indicator)
(Top row) Displays speeds in increments of 10 rpm at lower than 5,000 rpm, and in steps of 100
rpm at 5,000 rpm or more.
(Bottom row) Sets speeds from 1,000 to maximum speed in increments of 100 rpm. The lower two
digits (one, ten positions) display zeros.

• TIME (running time indicator)
(Top row) Displays the remaining operation time or the time elapsed during operation if settings
are performed on the User Customization screen. If the running time is set to HOLD, this field
displays time elapsed.
(Bottom row) Specifies a setting in the range from 1 minute to 99 hours 59 minutes in steps of
minutes and hours.

• TEMP (temperature indicator)
(Top row) Displays in steps of 0.1 °C.
(Bottom row) Sets a setting in the range from 0 °C to 40 °C in increments of 0.1 °C

• ACCEL (acceleration mode indicator). Displays acceleration modes 1 through 9.

• DECEL (deceleration mode indicator). Displays deceleration modes 1 through 9, along
with free coast (F).

(2) Function field • ID CODE Sets an ID code.

• RLM Switches to the Rotor Management screen.

• PROG • RTC Sets, recalls programmed runs or sets the time and the end time for a
programmed run.

• RCF • ω

2

T Sets and displays the centrifugal force or sets an ω2T

• DEF Sets and resets the defrost function.

• ZONAL Sets the zonal operation mode.

(3) Message indicator Displays an alarm message and various suggestions for operation.

(4)

Run mode indicator

The following terms are displayed:
STOP, ACCEL, RUN (running at the set speed)
DECEL, WAIT (waiting for vacuum during acceleration)
ZONAL (for zonal operation)

DELAY (until the start time in an RTC run)

(5) VACUUM indicator Displays the following four stages according to the vacuum of the rotor chamber.

(1) Atmospheric state. The vacuum pump is not activated.

(2) Low vacuum. The rotor waits at 4,000 rpm until the vacuum reaches an inter-

mediate level

(3) Intermediate vacuum.

(4) High vacuum.

Note If the sample is sensitive to a temperature rise, do not press the START key until the

chamber is at high vacuum level.

Description

Design

Thermo Scientific WX Ultra Series 1-5

Table 1-2 [Functions of keyboard-keyed by item no. to fig. 1-3]

No. Name and Symbol Functions and actions

(6) START key Starts rotor rotation. If VACUUM is off, this key activates the vacuum pump and starts

temperature control.

(7) STOP key Stops rotor rotation.

(8) VACUUM key Starts up the vacuum pump and activates air vent (As soon as vacuum pump is on, tem-

perature control starts.) Air vent for vacuum chamber after a run cannot be opened as
long as the rotor is spinning.

(9)

ESC key Moves the display back to the screen at the preceding level (for example, to switch

back from the Menu Screen to the Run Screen).

(10) MENU key Displays the Menu Screen.

The Menu Screen offers the choice of Centrifuge Scheduler, User List, Alarm Informa­tion, Rotor Catalog, and User Customization Routines.

(11) Cursor key a. Displays the cursor on the Run Screen, putting the display into input wait status.

b. Move the cursor on the screen.

1. Moves the cursor up

2. Moves the cursor down

3. Moves the cursor to the right

4. Moves the cursor to the left

(12) Numeric key Used to type numbers for setting run conditions.

During time and temperature entry: Moves cursor from hours to minutes.

Acts as decimal point for data entry.

a. During operation time entry: sets continuous run.

b. When entering deceleration conditions: sets a free coast.

Use this when you have entered the wrong value while entering an

operating condition or entering a number or when the alarm device is
activated.
Functions of this key
a. This key clears the cursor-carrying input field and returns you to the
preinput state.
b. Use this key to clear an alarm signal. If more than one alarm signal is
on, this key will clear them one by one.

Registers the entered value.

Description

Design

1-6 WX Ultra Series Thermo Scientific

Rotor chamber

The structure of the rotor chamber (vacuum chamber) is shown in Fig. 1-4.

Figure 1-4 Rotor chamber

Note If sample or water drops to the window of the temperature sensor, it may cause an incorrect

detection. Whenever the sensor is wet, wipe it with a clean, dry cloth. Take care not to scratch the
surface of the sensor.

Drive spindle

Speed sensor

Chamber door seal

Handle

Chamber door

Temperature sensor (measuring
the temperature of the rotor)

Door lock system

Vacuum chamber

Bowl (Rotor Chamber)

Thermoelectric cooling element
(cooling the rotor)

Guard

Overspeed detector
(detecting any instance
exceeding the maximum
allowable speed of the
rotor)

Description

Design

Thermo Scientific WX Ultra Series 1-7

Safety devices

(1) Containment

Should a rotor failure occur, the guard ring will contain the contents of the rotor inside the centrifuge
(Fig. 1-4).

(2) Imbalance detector

If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper
bucket setting, the imbalance detector detects the situation and decelerates the rotor immediately.
However, the ultracentrifuge is designed to tolerate imbalance associated with visual balancing-it is
equipped with an imbalance tolerant drive. (For more information on the balancing of rotors, see
Section 2-1-2, “Preparing tubes/bottles and rotor”.)

(3) Door lock system

The chamber door automatically locks for safety while the rotor is spinning. When the power supply is
off, the door remains locked. The door can only be opened and closed when the rotor is at rest and the
rotor chamber is vented. Unless the door is closed, the rotor will not start rotating except in zonal
mode. To open the door in the event of a power failure, see Section 2-7, “When power failure occurs”.

(4) Speed sensor and overspeed detector

For protection in the event of entry errors the ultracentrifuge is provided with an automatic system to
stop the rotor when its speed exceeds the maximum allowable speed. If a speed higher than the
maximum permitted speed is set, the ultracentrifuge will detect the mistake before the speed reaches
3000 rpm, and then will display an alert message and decelerate the rotor to a stop.

Description

Design

1-8 WX Ultra Series Thermo Scientific

Rotor adapter

Overspeed Decal

The overspeed decal located on the rotor base has alternating black and white bands. The number of
bands corresponds to the maximum permitted speed of the rotor. (See Fig. 1-5.)

Figure 1-5 Standard rotor

To protect the overspeed decal, be sure to store the rotor on the rotor stand provided. (See Fig. 1-6.)

Figure 1-6 Rotor stand

Overspeed decal

Standard rotor with
overspeed decal

Thermo Scientific WX Ultra Series 2-1

2

Operation

The Sorvall WX Ultra series ultracentrifuges are capable of operation in more than one mode to meet a
wide range of applications. The outline of each available mode is given below:

Outline Reference

Normal operation

Section Normal opera-

tion

Optional features

Programmed opera­tion

You can store set run conditions in memory for later use in
repeated operation.

Section How to use the

FUNCTION field

Section Programmed

operation

Step-mode operation

More than one normal operation can be combined into a
sequence of operations or step for successive centrifuga­tion.

Section Step-mode

operation

RTC (real-time con­trol) operation

Run starts or completes at a required date and time.

Section RTC (real-time

control) feature

RCF (centrifugal
force)
value display setting

This feature calculates centrifugal force (RCF) values from
set speed. It can also calculate reversely, i.e., finding
speed from such values.

Section Displaying and

setting RCF

Speed

Time

Speed

Speed

Time

Time

Store

Recall

Speed

Time

Speed

Time

A finish date
and time re­quired

START
key ON

Speed

Time

RCF

value setting

Operation

Run preparation

2-2 WX Ultra Series Thermo Scientific

Run preparation

Optional features

ω2T setting

Calculates and displays the running time from the set val-

ues of speed and ω

2

T.

Section Setting w2T.

Zonal operation

Zonal operation is a mode of operation using a zonal
rotor.

Section Zonal opera-

tion.

Speed

Time

Speed

Time

Zonal speed

WARNING

1. Never use any materials capable of producing flammable or explosive vapors, or extreme

exothermic reactions.

2. When using radioactive, toxic, or pathogenic materials, be aware of all characteristics
of the materials and hazards associated with them in the event leakage occurs during
centrifugation. If leakage does occur, neither the centrifuge nor the rotor can protect
you from particles dispersed in the air. To protect yourself, we recommend additional
precautions be taken to prevent exposure to these materials, for example, use of
controlled ventilation or isolation areas.

CAUTION Do not place fluids inside, on top of, or close to the centrifuge---spillage can result

in electrical or mechanical failure.

Operation

Run preparation

Thermo Scientific WX Ultra Series 2-3

Starting up the ultracentrifuge

Before setting run conditions, display the run screen (screen for setting run conditions)

(1) Displaying the Run Screen (screen for setting run conditions)

Figure 2-1

Initial screen, and run screen

Initial screen

1. Turn on the POWER switch.

2. The initial screen appears.

3. The Run Screen appears.

Operation

Basic operation

2-4 WX Ultra Series Thermo Scientific

Preparing tubes/bottles and rotor

The Sorvall WX Ultra series ultracentrifuges allow you to balance, by eye, tubes or bottles containing a
sample solution and then centrifuge them. Make sure that the difference between meniscus levels of
sample solution in tubes or bottles in within 5 mm (0.197 in) (See fig. 2-2).

Figure 2-2

Balancing tubes/bottles containing a sample solution

To prevent tube or bottle failure, some tube and rotor combinations cannot be run to the maximum
speed of the rotor when partially filled. The tube or bottle must be full in the following cases:

1. When a thin tube or seal tube is used.

2. When a thick tube is used for swinging rotor.

3. When a bottle is used 100,000 x g or more.

Basic operation

Setting run conditions

This section will first describe the screen for basic operation (the run screen) and the cursor keys.

Within 5 mm

(0.197 in)

Within 5 mm

(0.197 in)

(1) Tubes

(2) Bottles

WARNING Do not lean on, tilt or place anything on the centrifuge while in operation.

CAUTION

1. Do not operate the keyboard with a ball-point pen or a sharp object.

2. Do not continue to operate the centrifuge if abnormal sounds occur during operation.
Immediately discontinue use of the centrifuge and contact Thermo Service.

Operation

Basic operation

Thermo Scientific WX Ultra Series 2-5

[run screen]

The screen for displaying run conditions and operational status is called the run screen.
Speed, time, and temperature are displayed in two rows: the top row displays the current actual run
conditions, while the bottom row displays the set run conditions.
The acceleration (ACCEL) and the deceleration (DECEL) fields display set conditions.

Figure 2-3

Run screen

[Cursor key]

Pressing the cursor key will highlight the field where changes can be made. (This blinking/highlighted
object is referred to as the cursor in this manual.)
The screen setting field is in either of the following states depending on whether the cursor is there.

1. Determined input state: This is a normal state and the cursor does not appear.

2. Input wait state: Press a cursor key (either the top, bottom, right, or left) while in the determined
input state, and the numerical part of the setting field will blink a 0 (or numerical value) and
display the cursor. In this state, the system accepts a numerical input. Press cursor keys to move the
cursor.

To set a run condition, enter the cursor into an input wait state, move the cursor to the item you want
to set, then enter a value. If you have made no keystroke (such as a numerical input) for more than 30
seconds, the system will automatically enter a determined input state.

Note When the screen is in a determined input state without a cursor (when the Run Screen is on
after power-up), if you wish to enter a numerical value in a specified parameter field, press a cursor
key (either of the upward, downward, right, and left arrow keys), move the cursor to the specified
parameter field, then enter the value.
The cursor keys display and move a cursor. Once a cursor appears, pressing a cursor key moves the
cursor to the corresponding direction (upward, downward, rightward, or leftward).

Operation state
display field

Setting value
display field

Run mode indicator

}
}

}

Operation

Basic operation

2-6 WX Ultra Series Thermo Scientific

Figure 2-4 Setting indicator

How to set speed, running time, temperature, and other parameters

Here are some examples and descriptions:

Note

1. If you enter the wrong value, press CE key to return to the input wait state.
If you have pressed ENTER key, press a cursor key, enter the device into an input wait state,
then enter the correct value.

2. When setting two or more run conditions, you do not have to press ENTER key after each
setting. Pressing the cursor key will enter the setting, thus making the system wait for a new
input.

3. If the system is running in (HOLD) and you want to set it to shut down at a future time, enter
a new time setting while the instrument is in operation; enter the sum of the time elapsed plus
the time remaining. If, for example, this machine has run continuously for five hours and you
want to stop it one and a half hours later, use cursor keys to enter TIME into an

input wait state,

then enter

Setting item RPM (SPEED Running time (TIME)

Typical setting 100,000 rpm 2 hours 30 minutes

Cursor key

Cursor (blinking)

The system does not accept a
numerical input.

(1) Determined input state

The system accepts a numerical input.
Use cursor keys to move to the next
setting item.

(2) Input wait state

Operation

Basic operation

Thermo Scientific WX Ultra Series 2-7

Operation procedure

1 Press cursor keys to enter the system into an

input wait state.

The system enters an
input wait state.

2 hours 30 minutes

2 Use cursor keys to move the cursor to the

status indicator. (The arrows indicate the
directions the cursor can be moved..

The system enters an
input wait state.

3 The cursor in the setting item field blinks for

about 30 seconds.
Blinking means that the system enters an
input wait state.

4 Use numeric keys to enter a setting.

Entered numbers are moved to the left every
time a new number is entered.

The last two digits are
fixed.

Press the “ ” key to

move the cursor to the
“minutes” position.
For a continuous run,
press HOLD/FREE.

5 Make a check, then press ENTER.

After pressing a cursor key, you can still
enter a setting similarly to the ENTER key.
Use CE to cancel an input.

Set it to 100,000 rpm. Set it to 2:30

(2 hours 30 minutes).

Setting range and units Can be set to any value in

the range from 1,000 rpm
to maximum speed in
increments of 100 rpm.

Can be set to any value
up to 99 hours 59 minutes
in increments of 1 min­ute.

Arrow

Temperature (TEMP) Acceleration (ACCEL) Deceleration (DECEL)

4.5 °C 9 7

Enters an input wait state. Enters an input wait state. Enters an input wait state.

Operation

Basic operation

2-8 WX Ultra Series Thermo Scientific

The cursor blinks at one place.

When one decimal place is not
required, you do not need to enter

. If you press , it becomes
a “decimal place” input and the
machine waits for an input of deci­mal places.

For free coast, press HOLD/FREE.
Display: F

Set it to 4.5 °C. Set it to 9 Set it to 7.

Can be set to any value in the range
from 0 to 40 °C in increments of 0.1
°C.

1 to 9 1 to 9

+
free coast (F)

FUNCTION field

• The FUNCTION field
switches to the ID CODE
field.

• The number of units of ID
CODE blinks.

• The entered number is not
displayed on the screen. * is
displayed instead of the
number.

>> After the registered ID CODE is entered, the name (USER NAME) corresponding to

the ID CODE is displayed.

Operation

Basic operation

Thermo Scientific WX Ultra Series 2-9

Setting user ID code

The USER ID CODE is a number to identify each user and can be set in up to 4 digits.
When the user ID CODE is entered, the user record will be stored in the memory of the centrifuge and
can be printed if a printout operation is done (optional).
Entering a user ID CODE may not be always required for operation (Lockout feature optional). If the
centrifuge is not equipped with the optional lockout feature and the user does not need to be
identified, the centrifuge can be operated without an ID CODE.

• To use a name (user name) corresponding to the user ID code or ID code, it must be registered.
For the registration method, see Section User list.

Step Key operation Screen display and considerations

1 On the Run Screen, use cursor keys to

move the cursor to ID CODE and press
the ENTER key.

2 Use numeric keys to enter ID CODE

(4 digits).
(When entering 0123)

ID code

3 Press the ESC key on the keyboard to

return to the FUNCTION field.

• The display switches to the
rotor control field, which
displays the Rotor List
screen.

• The display switches back
to the Run Screen and
“T-8100-1234” is
displayed in the message
field.

Operation

Basic operation

2-10 WX Ultra Series Thermo Scientific

Using Rotor Life Management

The rotor type and serial number may be set in rotor management to allow for rotor logging within the
centrifuge. Whether the rotor type and serial number for a particular rotor have been set or not, it does
not affect the normal operation of the ultracentrifuge. However, you are advised to set the rotor type
and serial number of the rotor for each run to allow effective rotor life management. Any registered
rotor that does not have its rotor type and serial number set will appear as an undefined rotor in the
displayed list (“Undefined rotor 1”).
You can use a particular rotor as undefined if you need to manage the rotor separately from the other
defined rotors in terms of the number of runs made and accumulated run time (“Undefined rotor 2”).
For more information on rotor life management, see Section Rotor management.

Step Key operation Screen display and considerations

1 On the Run Screen, use cursor keys to

move the cursor to RLM and press the
ENTER key.)

2 On the rotor control field, use the left

and right arrow keys

and to select each page.

3 Use the up and down arrow keys to

move the cursor to the row of the rotor
type to be used and press the ENTER
key.

In this example, the cursor moves to
“T-8100-1234”.

Select “Running”.

Operation

Basic operating procedure

Thermo Scientific WX Ultra Series 2-11

Basic operating procedure

There are two basic modes of operation, normal and zonal. The procedures for these two modes are shown in Fig. 2-5.

START

Turn on the POWER.

Open the chamber door and

install the rotor.

Normal run

Close the door and set the run
conditions.

Press VACUUM.

For information on how to set
the run conditions, see
Section Basic operation

Vacuum pump is ON.
Temperature control starts.
Check the set run conditions
for accuracy.

Press START.

Rotor accelerates to the set speed, and then

high-speed centrifugation begins.

: Indicates panel key.

Zonal run

Reaches the set time,

or press STOP.

Rotor comes to a stop.

Press VACUUM.

Temperature control is OFF.
Vacuum pump is OFF.
Air valve is open and door lock
is released.

Remove the rotor and turn off

the POWER switch.

END

Operation

Basic operating procedure

2-12 WX Ultra Series Thermo Scientific

Figure 2-5 Basic operating procedure

Move the cursor to ZONAL
in the Run Screen.

Press 3, 0, ENTER

Set run conditions.

For information on how to
set the run conditions, see
Section Basic operation.

Press START.

Temperature control starts. The rotor
rotates at the zonal speed. Load the
sample, then cap the rotor.

Close the chamber door and
press VACUUM.

Vacuum pump is ON.
Check the set run conditions for accuracy.

* This step may be skipped. Just press START to
run the ultracentrifuge. When START is pressed,
the vacuum pump turns on and the rotor begins
to accelerate. Then the rotor stays at 4000 rpm
until the rotor chamber reaches predetermined
vacuum level.

Press START.

Rotor accelerates to the set speed.

Reaches the set time, or press STOP.

Rotor decelerates to
the zonal speed.
The buzzer sounds.

Press VACUUM and open
the chamber door.

Move the cursor to ZONAL in
the Run Screen.

Unload the sample.

Press 0, 0, ENTER

Rotor comes to a stop. Temperature
control is OFF.

Remove the rotor,
then turn off

the POWER switch.

END

Operation

Basic operating procedure

Thermo Scientific WX Ultra Series 2-13

Normal operation

Given below is a description of the operational procedure for a normal run.

Figure 2-6

Normal operation mode

Note Before starting up this machine, carefully read the operation manual for your rotor and make
sure that you have selected the correct type of tubes and entered the

correct amount of sample.

Speed

Tim e

Step Operation and keystrokes Unit operation and considerations

1 Turn on the POWER switch on this machine. >> The panel display lights up.

>> The door is unlocked.

2 Open chamber door and make sure chamber is

dry.

3 Install the rotor. >> Before installation, read the rotor instruction manual carefully.

4 Set run conditions. >> See Section Setting run conditions and set run conditions.

5 Press VACUUM key. (optional) >> The machine starts evacuating the rotor chamber.

>> Temperature control starts.
>> The degree of vacuum in the rotor chamber is displayed on the vacuum indicator
on the display panel.

>> If the rotor compartment has moisture or frost on it, it takes a long time to reach
a high vacuum.
In that case, wipe it off with a clean, dry cloth or sponge.
>> If the sample is sensitive to a temperature rise, do not press the START key
until the chamber is at high vacuum level.

6 Press START key >> The rotor starts spinning.

>> The timer begins operating.
>> The rotor accelerates to the set speed.
>> This ultracentrifuge waits at 4,000 rpm until an intermediate vacuum is
reached.

(1) In a low vacuum

(2) In an intermediate vacuum

(3) In a high vacuum

(1 indicator)

(2 indicators)

(3 indicators)

Operation

Basic operating procedure

2-14 WX Ultra Series Thermo Scientific

The run mode indicator on the display panel displays the following:

Figure 2-7

Displays of run modes

7 The specified centrifugation time elapses

(time-out). Or press STOP key.

>> The rotor decelerates and stops.

8 The rotor brakes or coasts to a stop. >> Beeps to indicate that the rotor has stopped.

9 Press VACUUM key. >> The vacuum stops, the air leak valve gets activates, and the rotor chamber

reaches atmospheric pressure.
>> The door unlocks, and is able to be opened and closed.

10 Take out the rotor.

Note Use the vacuum key to avoid prolonged vacuum wait time at 4,000 rpm when the ambient
temperature is low.

Speed (rpm)

Time

Operation

Basic operating procedure

Thermo Scientific WX Ultra Series 2-15

Zonal operation

Zonal operation is a mode of operation using a zonal rotor or continuous flow rotor for density
gradient centrifugation with large amounts of sample or continuous sedimentation of particles from a
large volume sample.

The following zonal rotors are available for operation in the Sorvall WX Ultra series ultracentrifuges:

TZ-28 sealed zonal rotor
TCF-32 continuous flow zonal rotor

The TZ-28 can only be operated in a sealed zonal mode. Dynamic loading/unloading or continuous
flow operation IS NOT allowed.
The TCF-32 zonal rotor can be operated in a continuous flow mode requiring dynamic loading and
unloading. The rotor comes with a kit to modify the Sorvall WX Ultra series.

The continuous flow zonal operation consists of the following three stages:

1. Centrifugation at low speed, called the zonal speed *, the gradient or sample being loaded in this stage.

2. Acceleration to set speed and separation of the sample, or

3. Centrifugation at zonal speed, the sample and or gradient being unloaded in this stage.

Figure 2-8

Zonal operation mode

* Zonal speed: The zonal speed is one that is required for loading and unloading the sample.
The zonal speed is normally set at 3,000 rpm, but it can be set in the range from 2,000 to 3,000 rpm in
increments of 100 rpm to obtain the required speed. For details of how to change zonal speed, see
Section User Customizations.

The following explains how to perform a zonal run.

* Zonal speed

Tim e

Speed

WARNING When operating the centrifuge with the door open and the rotor spinning,
eliminate all possibility of contact with the rotor. Remove any objects not essential to
operating procedures which could either catch on or drop into the spinning rotor (that is,
neckties, rings, bracelets, watches, long sleeves, or any loose articles in breast pockets).

Step Operation and keystrokes Unit operation and considerations

1 Install the zonal rotor on the drive spindle. >> See zonal rotor manual.

•The characters ZONAL
are displayed in the
message display field on
the screen and the zonal
operation mode is set.

Operation

Basic operating procedure

2-16 WX Ultra Series Thermo Scientific

2 On the Run Screen, move the cursor to ZONAL

and enter the following by operating numeric
keys to set the zonal operation mode.

3 Set the run conditions. >> For details, see Section Basic operation.

4 Press the START key.

Note The time being consumed at zonal speed is not counted as
part of the run.

5 Install the seal assembly on the rotor. >> For details, see zonal rotor manual.

6 Load the sample and the gradient solution.

7 Install the cap assembly on the rotor.

8 Close the chamber door.

9 Press the VACUUM key. (This step may be omit-

ted.)

>> Vacuum pump begins to work.
>> Chamber door is locked.

10 Press the START key again. >> Rotor begins to accelerate to the set speed.

Note The run time is counted from when the START key is pressed. The

instrument can also count only the time elapsing while the rotor is
spinning at high speed. This is possible by changing the run time setting
range. For details, see Section “Run time range setting”

on page 2-80.

11 If you need to stop the run before the set time

elapses, press the STOP key.

>> Rotor is decelerated to the zonal speed and then the buzzer sounds.

12 Press the VACUUM key. >> Vacuum pump stops working and air enters the rotor chamber.

>> Door lock is released.

• Rotor begins to accelerate
to the zonal speed.

• Temperature control
begins.

Operation

Acceleration and deceleration rates

Thermo Scientific WX Ultra Series 2-17

Acceleration and deceleration rates

In order to meet various experimental protocols, the acceleration and deceleration rates can be adjusted
between 0 to 500 rpm.
The figure and table below show the relationship between ACCEL/DECEL code numbers selected and
resulting approximate acceleration/deceleration times.

13 Open the chamber door. >> For details, see zonal rotor manual.

14 Remove the cap assembly

15 Install the seal assembly and unload the sam-

ple.

16 On the Run Screen, move the cursor to ZONAL

in the FUNCTION field and enter the following
by operating numeric keys to set the normal
operation mode.

>> Rotor decelerates to a stop.

Note During the zonal mode, the rotor will not decelerate even if the

STOP key is pressed during the rotor's spinning at the zonal sped.

17 Be sure that the rotor is at rest, then remove the

rotor.

>> If the rotor is still spinning, do not remove it. Wait until it comes to a complete
stop.

Note By changing to the NORMAL mode when loading and unloading the sample, you can
decelerate the rotor to a stop.

ACCEL/DECEL code numbers

Tim e

Speed (rpm)

Operation

Acceleration and deceleration rates

2-18 WX Ultra Series Thermo Scientific

* Minimum time is the time for accelerating or decelerating by the driving motor with maximum
torque. this time depends on the type of rotor in use.

The following are typical examples of application of acceleration and deceleration rates

Code No.

Acceleration time (minutes) from rest to
500 rpm

Deceleration time (minutes) from 500
rpm to rest

9 Minimum time* Minimum time*

81 1

72 2

63 3

54 4

45 5

36 6

27 7

18 8

F - Coasting deceleration

Suggested code nos.

Characteristic of separation

ACCEL DECEL

Density gradient centrifugat ion
using a vertical rotor

5 7 The sample and gradient in tubes reorient during accel-

eration and deceleration. Therefore, the sample and
gradient can become mixed, especially in wide tubes, if
you use rapid acceleration or deceleration.

DNA separation by CsCl iso­pycnic separation (self-forming
gradients)

9 7 You can operate at maximum acceleration because the

density gradient is not formed during the run. As for the
deceleration, it is better to decelerate slowly to obtain
sharp bands.

Pelleting using a fixed angle
rotor

9 9 Rapid pelleting of samples is possible (the run time

decreases).

Density gradient centrifugat ion
using a swinging bucket rotor

8 8 The sample and gradient do not reorient. Therefore,

mixing of the layers is less than that in the case of using
a vertical rotor.

Note For a swinging bucket, there is no difference with regard to turbulence if ACCEL/DECEL is
less than or equal to 8. However, when the mode for long acceleration time is selected, (1, 2, 3), an
imbalance alarm indicator may light.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-19

Figure 2-9 Operation with setting ACCEL = 0 and DECEL = 0

How to use the FUNCTION field

This ultracentrifuge incorporates a number of features. These features are displayed and specified in the
FUNCTION field.
The FUNCTION field is extended as shown in the following figure.

Figure 2-10

FUNCTION field

Allowable maximum speed

500 rpm

2 minutes

15 minutes

15 minutes 2 minutes

Speed

ENTER key

ENTER key

Operation

How to use the FUNCTION field

2-20 WX Ultra Series Thermo Scientific

The above features can be used in combination.
When all settings are entered, press ESC to move back to the Run Screen. Then enter a setting for
another feature to form a combination.

ID CODE:

Sets an ID code.

Figure 2-11 Rotor radius

RLM:

Switches the Run Screen to the rotor management field.

PROG:

Programs, stores, and recalls run conditions. This feature
also offers a step-mode operation: a continuous run of
multiple run conditions.

RTC:

Sets a start time or a finish time and runs the ultracentrifuge
at a desired date and time.

RCF

max

: Maximum centrifugal force for the maximum radius r

max

of
the rotor used. This feature is used to cause the system to
automatically calculate and display RCF

max

. It also sets an

RCF

max

value and calculates the speed.

RCF

avg

: Causes the system to automatically calculate and display the

average centrifugal force RCF

avg

for the average radius r

avg

of the rotor used. It also sets an RCF

avg

value and calculates

the speed.

ω2T: Performs an rω2T run and arithmetic operations.

DEF:

Turns on and off the defroster.

ZONAL:

Selects between zonal operation and normal operation.

Note To perform a combination of PROG and RTC, first set PROG and then set RTC. Once
RTC is activated, you cannot change the run time. You therefore cannot activate PROG.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-21

Programmed operation

This ultracentrifuge has a programmed operation feature that stores run conditions. Storing run
conditions which you often use allows you to recall those conditions as often as you wish, thus saving
time in setting. (The saved run conditions will remain in effect even after the power switch is turned
off.)
This centrifuge incorporates the program areas indicated below. It has twenty memory areas and nine
steps in each memory number.
Accordingly, twenty patterns of run conditions can be stored and each memory can store nine steps.
Running this machine with each memory number retaining multiple steps will allow you to change the
speed, run time, temperature, and others while in operation. (Step-mode operation).

Figure 2-12

Program areas

Operation

How to use the FUNCTION field

2-22 WX Ultra Series Thermo Scientific

Basic operation of the programmed operation feature

Use cursor keys to

select and

press ENTER key.

ENTER key

ENTER key

ENTER key

Memory unit number
ENTER key

Next page

Press ESC several times to go
back to the Run Screen.

Programmed operation
possible

Enter a memory
number.

If you know which memory number
stores the run condition.

Use cursor keys to

select and

press ENTER key.

To store, change or delete a
program, or if you have forgotten
the memory number where the run
conditions are stored.

Press key to cause

to blink and

press ENTER key.

Enter a desired memory number and
press ENTER key.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-23

Note You cannot create, change, or delete a program while in running. Perform these operations
while not in running. However, you can search the memory screen every time.

Operation

How to use the FUNCTION field

2-24 WX Ultra Series Thermo Scientific

1. Programming procedure for run conditions (creating or changing)

Shown below is the procedure for storing (creating) or changing a run condition.

Step Key operation Screen display and considerations

1 While in the Run Screen, use the cur-

sor keys to move the cursor to PROG •
RTC and press ENTER key.
Then, move the cursor to PROG and
press ENTER key.

2

Press the upward cursor key to
cause PROGRAM to blink, then press

ENTER key.

>> If you wish to store a new condition, enter it into an empty memory number.

3 Use numeric keys to enter a memory

number you wish to store (or change).
Example: To store a condition in mem­ory number 3, press

4 Select “Creating or Changing.”

• The FUNCTION field
switches to the PROGRAM
field.

• The MEMORY No. setting
field blinks.

• The display switches to the
Program Map screen and the
MEMORY No. selection
field blinks.

• The memory screen you
have just specified appears.

• The message field in the bottom
row switches to what is shown in
the figure to the left.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-25

5

Enter a desired condition.
For example, select “SPEED”.

6 Select a desired rotor.

To select a desired rotor from the
“Used Rotor List”:

To select a desired rotor from the
“Rotor Catalog”:
(1) Select “Rotor Catalog”.

(2) Select Fixed Angle Rotor in the rotor
types.
(When using fixed angle rotor T-880)

(3) Select fixed angle rotor T-880.

(4) Select “Yes”.

• The display switches to the
Choose Rotor screen.

• The display switches to the Used
Rotor List screen.

• You can search for the rotor
number you need in the order
starting with the latest date/time.

• The display switches to the Select
Rotors screen.

• The display switches to the Fixed
Angle Rotor list screen.

• The specifications of the T-880
are displayed.

Operation

How to use the FUNCTION field

2-26 WX Ultra Series Thermo Scientific

6 To select a desired rotor from the

“Rotor Management”:
(1') Select “Rotor Management”.

(2') Select a desired rotor using the
upward or downward cursor key

.

(When using fixed angle rotor T-8100)

(3') Select “Yes”.

To select no rotor:
Select “None”.

7 Enter values into SPEED (RCF

max

or

RCF

avg

or ω2T), TIME, TEMP, A

(ACCEL), and D (DECEL).

>> To enter run conditions in multiple steps in a step-mode operation, continue
the operation from the above. (See Section Step-mode operation.)
>> You cannot skip a step. (You cannot enter anything in step 2 with nothing
entered into step 1.)

• The display switches to the Rotor
Management screen and the
Rotor List is displayed.

• The rotor type you have just
entered appears in the top row in
the screen.

• The SPEED field in the SPEED
priority screen blinks.

• The SPEED field in the SPEED
priority screen blinks.

Example

(Downward cursor key)

• When you finish entering run
conditions for step 1, step 2 enters
into an input wait state.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-27

To change a run condition, use cursor
keys to move to the item you want to
change and then enter a value.

8

Press the downward cursor key
to blink the value part of the message

field in the bottom row. Then select
“Yes”.

Yes

No

>> If you select “Yes”:

>> If you select “No”, the message field in the bottom row switches to what you
see in process 4. Select a new setting.

9 After entering memory run conditions,

perform an entry operations for User
Name.
(1) Press the ESC key.

(2) When attaching User Name to the
memory run conditions, select “Yes”.

>> When you press ESC key, the bottom row message is displayed.
(1) A selection field as to whether User Name should be input or deleted appears.

(2) A selection field for selecting either inputting or deleting appears.

(3) Select “Inputting User Name”.

(4) Enter 4 digits of the ID CODE that is
already registered.

(5) Check User name and select “Yes”.

(3) The display switches to the ID CODE input field.

(4) The display switches to the User Name check field.

(5) The display switches to the Registration completion message field.

• This diagram shows what you will
get if you move the cursor and
enter the TIME field into an
input wait.

• The message field in the bottom
row switches to what is shown in
the diagram to the left, and the
storing of the run conditions in
memory number 3 is completed.

ID code

Operation

How to use the FUNCTION field

2-28 WX Ultra Series Thermo Scientific

10 Add Remark to the run conditions in

the memory.
(1) Press the ESC key.

(2) To add Remark to the run conditions
in the memory, select “Yes”.

(3) Select “Inputting Remark”.

(4) Enter Remark in 8 characters or
less.

(5) After entering the characters for
Remark, move the cursor to SET and
press the ENTER key to register the
character string.
(6) Check the contents of Remark and
select “Yes”.

>> When you press ESC key, the bottom row message is displayed.
(1) A selection field to input or delete Remark appears.

(2) A selection field to select either inputting or deleting appears.

(3) The display switches to the Remark registration field.

(4) Remark is entered from the left.

(5) The display switches to the Remark check field.

(6) The display switches to the registration completion message field.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-29

11 When the storing is over, press ESC

key several times.

>> The first push on ESC key switches the message display in the bottom row to
what you see in process 3 as shown below. To run this centrifuge, select Run­ning.

>> Pushing ESC key a second time displays the Program Map screen shown in
process 3.

>> The third push on ESC key switches you back to the Run Screen (program
screen).

>> The fifth push on ESC key switches you back to the Run Screen (FUNCTION
field).

• Make sure that the condition is
stored in memory number 3.

Note

1. If you make and store changes in a memory area that already stores run conditions, the
previous conditions are replaced by the new conditions.

2. You cannot store a run condition while running (while the rotor is spinning). Always
perform this function while not running.

Operation

How to use the FUNCTION field

2-30 WX Ultra Series Thermo Scientific

2. How to perform a programmed operation

Shown below is how to perform a “programmed operation”, that is, how to recall a stored set of run
conditions and run this centrifuge accordingly.

(a) If you know which memory unit number you need

Step Key operation Screen display and considerations

1 Turn on the POWER switch on the cen-

trifuge.

>> The panel display appears.
>> The door unlocks.

2 Install a rotor. >> Install the rotor securely on the shaft.

3 While in the Run Screen, use cursor

keys to move the cursor to PROG • RTC
and then press ENTER key.
Next, move the cursor to PROG and
press ENTER key.

4 Enter the memory number you wish to

operate.

Example: to call memory number 3,
press

>> The message field in the bottom row displays PROG.
>> If the STEP field displays what is shown below, this means that the memory
unit you have just called stores multiple run conditions (stepmode feature). For
details, see Section Step-mode operation.

>> The system only accepts numbers 1-20.

5 Run the centrifuge under normal oper-

ation without making changes to the
run conditions.

>> Run this machine according to Normal operation.
>> If you make changes to the run conditions (such as SPEED and TIME) after call­ing a program, the program you have just called is canceled. You must call it
again to use it.

• The FUNCTION field switches
to the PROGRAM field.

• The MEMORY No. setting field
blinks.

• The run conditions in memory
appear in the respective fields.

In the Run Screen, the run conditions of
this step are displayed (the first step

in this case).

Final step

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-31

(b) If you do not know which memory number you need

Step Key operation Screen display and considerations

1 Turn on the POWER switch of this

machine.

>> The panel display appears.
>> The door unlocks.

2 Install a rotor. >> Install the rotor securely on the shaft.

3 While in the Run Screen, use cursor

keys to move the cursor to PROG • RTC
and then press ENTER key. Then, move
the cursor to PROG and press ENTER
key.

4

Press the upward cursor key to
blink PROGRAM. Then press ENTER

key.

5 Use numeric keys to enter the memory

number where you have stored data.

To call memory number 3, press

>>If the contents of the memory number you have just recalled is not what you
want, press ESC to switch back to the Program Map screen and then call another
memory unit number.

6 If you get the memory number you

want, select Running.

• The FUNCTION field switches
to the PROGRAM field.

• The MEMORY No. setting field
blinks.

• The display switches to the
Program Map screen and the
MEMORY No. selection field
blinks.

• The image of the specified
memory number appears.

• The message field in the bottom
row switches to what you see
below.

Operation

How to use the FUNCTION field

2-32 WX Ultra Series Thermo Scientific

Step-mode operation

This ultracentrifuge incorporates a step-mode operation feature, which stores two or more run
conditions in one program memory area and switches between different values of speed, running time,
temperature, and other parameters while in operation. This centrifuge can store up to nine steps.
This section explains how to make settings by showing some examples.

(1) How to activate a step-mode operation

[Typical settings]
Shown below is the example of a three-step run and how to activate a step-mode operation.

7 To run the centrifuge, select “Yes”.

Yes

No

>> If you select “Yes”

>> The message field in the bottom row displays PROG.
>> If the STEP field displays what is shown in the figure below, that means that
the memory number you have just called stores multiple run conditions
(step-mode feature). For details, see Section Step-mode operation.

>> If you select “No”, the message field in the bottom row displays what you see
in process 5. Select a new condition.

8 Start running the centrifuge under nor-

mal operation with no changes made
to the run conditions.

>> Run this machine according to Section Normal operation.
>> If you make changes to the run conditions (such as SPEED and TIME) after
recalling a program, the program you have just recalled will be canceled. You
must recall the program again to use it.

• The display switches back to the
Run Screen.

• The run conditions in memory
appear in the respective fields.

In the Run Screen, the run conditions of
this step are displayed (the first step in
this case).

Final step

Note

1. To check the contents (run conditions) of the memory number for the program while in
running, follow processes 3 through 5 in (b) “If you do not know which memory unit number
you need.” After the check, press ESC to get back to the Run Screen.

2. To perform a combination of a programmed run with RTC (real-time control) (see Section

RTC (real-time control) feature), first recall a programmed memory number, then set RTC.

The system will then calculate the total of the running times of all steps of the programmed
run and calculate the start time for RTC. Therefore, you cannot recall the program memory
after setting RTC.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-33

Figure 2-13 A typical step-mode run

Run time

Step 1

Step 2

Step 3

Speed (rpm)

Operation

How to use the FUNCTION field

2-34 WX Ultra Series Thermo Scientific

Step Key operation Screen display and considerations

1 While in the Run Screen, use the cur-

sor keys to move the cursor to PROG •
RTC and press ENTER key.
Then move the cursor to PROG and
press ENTER key.

2

Press the upward cursor key to
cause PROGRAM to blink, then press

ENTER key.

3 Use numeric keys to enter the memory

unit number you want to store.

To store data in memory number 3,
press

• The FUNCTION field switches
to the PROGRAM field.

• The MEMORY No. setting field
blinks.

• The display switches to the
Program Map screen and the
MEMORY No. selection field
blinks.

• To store a new condition, enter it
into an empty memory number.

• The image of the specified
memory number appears.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-35

4 Select “Creating or Changing.”

5

Enter a desired condition.
To select SPEED, press

To select no rotor, press.

>> To select and enter RCF

max

or RCF

avg

, see Section 1. Programming procedure for

run conditions (creating or changing)

6 Specify run conditions for step 1.

>> If you press the cursor key (downward) after setting numeric value for
DECEL (D) in step 1, setting the run conditions in step 1 finishes and step 2 enters

into an input wait state.

7 Then specify run conditions for step 2.

• The message field in the bottom
row switches to what you see on
your left-hand side.

• The display switches to the

Choose Rotor screen.

• The display switches to the

SPEED priority setting field and
the SPEED area blinks.

Downward cursor key

• The entry of run conditions for

step 1 is complete.

Downward cursor key

• The entry of run conditions for

step 2 is complete.

Operation

How to use the FUNCTION field

2-36 WX Ultra Series Thermo Scientific

8 Lastly, specify run conditions for step

3.

9

Press the downward cursor key
to blink the value part of the message

field in the bottom row. Then select
“Yes”.

Yes

No

>> If you select “Yes”:

If you select “No”, the message field
in the bottom row switches to what
you see in process 4. Input a new
setting.

10 After entering step-mode run condi-

tions, perform an entry operations for
User Name.

(1) Press the ESC key.

(2) When attaching User Name to the
memory run conditions, select “Yes”.

(3) Select “Inputting User Name”.

(4) Enter 4 digits of the ID CODE that is
already registered.

(5) Check User Name and select “Yes”.

>> When you press ESC key, the bottom row message is displayed.
(1) A selection field as to whether User Name should be input or delete appears.

(2) A selection field for selecting either inputting or deleting appears.

(3) The display switches to the ID CODE input field.

(4) The display switches to the User Name check field.

(5) The display switches to the Registration completion message field.

Downward cursor key

• The entry of run conditions for
step 3 is complete and step 4 is
ready for input.

ID code

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-37

11 Add Remark to the run conditions in

the memory.
(1) Press the ESC key.

(2) To add Remark to the run conditions
in the memory, select “Yes”.

(3) Select “Inputting Remark”.

(4) Enter Remark in 8 characters or
less.

(5) After entering the characters of
Remark, move the cursor to SET and
press the ENTER key to register the
character string.

(6) Check the contents of Remark and
select “Yes”.

>> When you press ESC key, the bottom row message is displayed.
(1) A selection field to input or delete Remark appears.

(2) A selection field to select either inputting or deleting appears.

(3) The display switches to the Remark registration field.

(4) Remark is entered from the left.

(5) The display switches to the Remark check field.

(6) The display switches to the registration completion message field.

12 Press ESC key when the storing is over.

13 Select “Running”.

• The message field in the bottom
row switches to what is shown in
the diagram on your lefthand
side, and the storing of the run
conditions in the appropriate
memory number is completed.

Operation

How to use the FUNCTION field

2-38 WX Ultra Series Thermo Scientific

1. Step display

The PROGRAM field displays steps as follows:

When the system finishes separating step 1 and moves to step 2,

When the system moves from step 2 to the final step 3,

Thus, you can see at a glance how many steps are stored in the specified memory and which step the
centrifuge is following.

2. While the instrument is running (while the rotor is spinning), you cannot store a stepmode run
condition. Always perform this function while not in running.

3. To check the contents (run conditions) of a step-mode program memory number, follow processes
1 through 3. To get back to the Run Screen after the check, press ESC key.

4. To perform a combination of a step mode run with an RTC (real-time control) run (see Section

RTC (real-time control) feature), call a program memory number, then set RTC.

The system then calculates the total of running times of all steps of the programmed run and
calculates the startup time for RTC. You therefore cannot call a program memory number after
setting RTC.

14 To run the centrifuge, select Yes.

Yes

No

>> If you select “Yes”:

>> The message field in the bottom row displays PROG.

>> If you select “No”, the message field in the bottom row displays what
you see in process 10. Make the selection again.

15 Start running the centrifuge under nor-

mal operation with no changes made
to the operational conditions.

>> Run the centrifuge according to Normal operation.
>> If you make changes to the run conditions (such as SPEED and TIME) after call­ing a program, the program you have just called gets canceled. You must call the
program again.
>> Pressing START makes the rotor rotate under the run condition of step 1.
>> When step 1 ends, the system moves to steps 2 and 3 automatically.

• The display switches back to the

Run Screen.

• The run conditions of step 1 in
memory appear in the respective
fields.

In the Run Screen, the run
conditions of this step are
displayed (step 1 in this case).

Final step

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-39

(2) Deleting a program

This section explains how to delete a set of run conditions stored in program memory.
To delete a memory number, delete all steps in that memory number.

Note While the instrument is running, you cannot delete a program memory number.

Step Key operation Screen display and considerations

1 While in the Run Screen, use cursor

keys to move the cursor to PROG • RTC
and press ENTER key.
Then move the cursor to PROG and
press ENTER key.

2

Press the upward cursor key to
cause PROGRAM to blink, then press

ENTER key.

3 Use numeric keys to enter the memory

unit number you want to delete.

To delete memory unit 3, press

4 Select “Deleting”.

• The FUNCTION field switches
to the PROGRAM field.

• The MEMORY No. setting field
blinks.

• The display switches to the
Program Map screen and the
MEMORY No. field blinks.

• The image of the memory unit
you have just specified appears.

• The message field in the bottom
row switches to what you see on
your left.

Operation

How to use the FUNCTION field

2-40 WX Ultra Series Thermo Scientific

(3) Other procedures

1. Making changes to the run conditions
Call the memory number you want to make changes to and make the changes. Alternatively, delete
the memory number, then store a new set of run conditions.
To make a change that will result in fewer steps, delete the memory number and then enter a new
set of run conditions and store them.
While in a programmed run, modification of a run condition is limited to the step which is
currently running.

2. Running the centrifuge starting from an intermediate step
You cannot run the centrifuge starting from an intermediate step in a memory number that
contains multiple steps.
Store (register) run conditions for the intermediate step and later steps in another memory
number. Then recall the memory number and run it.

3. What if a “SPEED” alarm goes on?
If a step stores a speed exceeding the maximum allowable speed of your rotor, the system will detect
it in the step 1 run and display the “SPEED” alarm.
Double-check the speed of all steps and correct any wrong ones.

4. Stopping the centrifuge in operation
Press STOP. The rotor stops and the system does not move to the next step.

RTC (real-time control) feature

The Sorvall WX Ultra series ultracentrifuges contain an internal clock, allowing you to run the
machine at a specified start or finish time for centrifugation. This feature for running the machine at a
specified time is called the RTC (real-time control) feature.
Below is an example of how to perform an RTC run.

Example: If you wish to install your rotor on the centrifuge under the run conditions listed below on
the evening of October 1 and take the samples out around 9:30 a.m. the next morning;

1. Rotor: T-8100

2. RPM: 100,000 rpm

3. Separation time: 4 hours

5 To delete this memory number, select

Yes.

Yes

No

>> If you select “Yes”.

>> If you select “No”, the screen switches to what you see in process 3. Make the
selection again.

6 Press ESC several times to move back

to the Run Screen.

>> If you delete a memory number, you can no longer run the centrifuge according
to that number.

• The system deletes all run
conditions in memory number 3.
(While in step mode, all steps get
deleted.)

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-41

4. Control temperature: 4 °C

5. Acceleration mode: 9

6. Deceleration mode: 7

Figure 2-14 A typical RTC run

In this example, you set the above run conditions (2) through (6), set the finish time for RTC run to
9:30 a.m., October 1 and start the centrifuge.
(You can make an identical setting by setting the start time to 5:30 a.m. instead of setting the finish
time to 9:30 a.m.)

100,000

Speed (rpm)

START key ON

4:30 p.m, October 1

Automatic start at 5:30 a.m., October 2

4 h

Stop around 9:34 a.m.

Finish time

9:30 a.m.

Operation

How to use the FUNCTION field

2-42 WX Ultra Series Thermo Scientific

1. How to perform an RTC run
Step Key operation Screen display and considerations

1 Set operational conditions while in the

Run Screen.
Use cursor keys to enter the SPEED
part into an input wait, then press

Move the cursor to PROG • RTC and
press ENTER key.

2 Move the cursor to RTC and press

ENTER key.

3 Select the item you wish to set. To

select the “Setting stop time”, press

>> Setting the “Setting stop time”

>> Setting the “Setting start time”

• The display switches to the
RTC Setup screen.

• The “dd” item blinks.
“mm” and “dd” indicate the
current date as a default.

• If you do not make any
input for 30 seconds, the
cursor disappears, and you
cannot make an input. At
that time, press a cursor key.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-43

4 Use cursor keys and numeric keys to

enter month, day, hours, and minutes.

Then, press ENTER key.

>> To set the hours, use a number between 0 and 23 (in the 24-hour system).
>> Enter a time later than the current time.
When setting the “Setting stop time”, allow for centrifugation time and set the
centrifugation start time to a time later than the current time.
>> You cannot set the centrifugation start time to a date more than 20 days
ahead.

5 If you are sure that you have made the

correct settings, select Yes.

Yes

No

>> If you select “Yes”

>> If you select “No”, you will get the display you see in process 2.
Make the selection again.

6 Check the RTC setting in the Run

Screen, then press START key.

The RTC run does not get activated
unless you press START key.

>> Run this machine according to Section Normal operation.
>> Once the RTC setting is made, you cannot change the run time (centrifugation
time).
If you wish to make a change to the run time, cancel RTC first.
>> Pressing START enters the system into a “DELAY” (and vacuum comes on),
condition causing the centrifuge to wait until the set time comes. This centrifuge
will turn the rotor automatically at the specified time and run it for the specified
period.

• The display switches to the
Run Screen and the
specified RTC time
appears.

• The message field displays
RTC.

• The run mode in the message
display field switches to
“DELAY”.

• Blink the PROG • RTC
in the FUNCTION field.

• To specify the run time
(centrifugation time), do
not set HOLD. Use ten
keys to enter the run time
instead.

Operation

How to use the FUNCTION field

2-44 WX Ultra Series Thermo Scientific

1. For an RTC run, the run mode indicator on the display panel displays the following:

Figure 2-15

Run mode display (RTC)

2. You cannot make an RTC setting in any of the following cases:

a. When the Run Screen is set to HOLD (continuous run)

Set the run time (centrifugation time) not to HOLD but to a numerical value.

b. When it is past the start time

Set the start time to a time later than the current time.

c. When the start time is more than 20 days after the current time

Set the time to a time no more than 20 days afterwards.

3. To change the run time (centrifugation time) after making an RTC setting, cancel RTC and then
set a new run time.

4. To perform a combination of a programmed operation (including a step-mode operation) with an
RTC run, call a program memory number, then set RTC.
The system calculates the total run times of all steps of the programmed operation and calculates
the start time for RTC.
Therefore, you cannot recall the program memory number after setting RTC.

5. To stop this ultracentrifuge in RTC operation, press STOP key. The system then stops RTC and
stops the rotor.

Speed (rpm)

START key ON

Automatic start

Tim e

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-45

Making changes to the RTC settings

This section shows how to cancel or make changes to the RTC settings you have made.
If you have already started an RTC run, press STOP to abort the RTC first. Press STOP to cancel the
RTC run.

Step Key operation Screen display and considerations

1 (1) While in the Run Screen, use cursor

keys to move the cursor to PROG • RTC
and then press ENTER key.

(2) Use cursor keys to move the cursor
to RTC and then press ENTER key.

>> The message indicator displays RTC.

2 Press cursor keys to blink RTC and then

press ENTER key.

• The FUNCTION field
switches to the RTC field.

Operation

How to use the FUNCTION field

2-46 WX Ultra Series Thermo Scientific

Canceling the RTC settings

The canceling procedure varies depending on whether the RTC run is started (START is pressed) or

not.

3 Making a change to date and time set-

tings
(1) Select “Setting stop time” or “Set­ting start time”.
To change the “Setting stop time”,
press

(2) Perform process 4 and later ones in

1. How to perform an RTC run men-

tioned above and change the date and
time settings.

See Section 1. How to perform an RTC run.

• The screen switches to a
screen where you can enter a
stop time (or start time).

Step Key operation Screen display and considerations

1 (1) While in the Run Screen, use cursor

keys to move the cursor to PROG • RTC
and then press ENTER key.

(2) Use cursor keys to move the cursor
to RTC and then press ENTER key.

>> The message indicator displays RTC.

• The FUNCTION field
switches to the RTC field.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-47

2 Press cursor keys to blink RTC and then

press ENTER key.

3 [Before starting the RTC run]

(1) Select “Deleting the set RTC”.

(2) Select “Yes”.

>>If you select “Yes”

>> If you select “No”, you will get the display you see in process 2. Make a selec­tion again.

[After starting the RTC run]
(1') Select “Deleting the set RTC”.

(2') i) Select “Cancel RTC-NO run”.

• The message indicator in the
bottom row changes.

• The RTC setting has been
canceled.

• The message indicator in the
bottom row changes.

Operation

How to use the FUNCTION field

2-48 WX Ultra Series Thermo Scientific

Displaying and setting RCF

This ultracentrifuge stores the maximum and average radii of each rotor in internal memory. Setting a
speed causes this centrifuge to automatically calculate and display the RCF (relative centrifugal force)
value, while setting an RCF value causes the centrifuge to automatically calculate and display the speed.
Given below is a description of how to display and set RCF.

1. How to display an RCF value

• When the rotor to be used is already identified

(when rotor type is displayed in the message display

field)

ii) Select “Cancel RTC-Start run”.

iii) Select “Continue RTC”.

(3) Press ESC after process (2') i) or ii).

>> The RTC in the message indicator in the bottom row disappears.

• The display switches back to
the Run Screen.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-49

Step Key operation Screen display and considerations

1 While in the Run Screen, set a speed. >> See Section Setting run conditions.

2

Use cursor keys to move the cursor to

RCF • ω

2

T and press ENTER key.

3 Move the cursor to RCF

max

(or RCF

avg

)

and press ENTER key.

>> The RCF value associated with the set speed you have just entered in process
1 appears in the RCF setting field.

•The FUNCTION field
switches to what is shown in the
diagram to the left, and the
RCF

max

field blinks.

• The RCF display field displays
the RCF value of the rotor.

RCF display field

RCF setting field

Note Press ESC key to cancel the RCF field.

Operation

How to use the FUNCTION field

2-50 WX Ultra Series Thermo Scientific

• When the rotor to be used is not identified (when rotor type is displayed in the message display
field)

Step Key operation Screen display and considerations

1 Set the speed on the Run Screen. >> See Section Setting run conditions.

2 (1) Use cursor keys to move the cursor

to RCF • ω

2

T and press the ENTER

key.

(2) Use cursor keys to move the cursor to
RCF

max

(or RC F

avg

) and press the ENTER

key.

3 Check that ROTOR LIST blinks and

press the ENTER key.

• The contents of the display in
the FUNCTION field
change.

•The RCF

max

area blinks.

• The FUNCTION field
switches to the RCF field.

• ROTOR LIST blinks.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-51

4 Select a desired rotor. To select a

desired rotor from the “Used Rotor
List”:

To select a desired rotor from the
“Rotor Catalog”:
(1) Select “Rotor Catalog”.

(2) Select Fixed Angle Rotors in the
rotor types.
(When using fixed angle rotor T-890)

(3) Select fixed angle rotor T-890.

4 (4) After checking the specifications of

the rotor, select “Yes”.

>> When you select “Yes”

• The RCF value of the running rotor is displayed in the RCF display field.

>> When you select “No”, the display goes to the same as the display of process
4 (2).

• The display switches to the
Used Rotor List screen.

• You can search for the rotor
number you need in the
order starting with the
latest date/time.

• The display switches to the
Rotor List screen.

• The fixed angle rotor list is
displayed.

• The specifications of the
T-890 rotor are displayed.

• The display switches back
to the Run Screen and the
selected rotor type is
displayed in the message
display field of the bottom
row.

RFC display field

•

The display switches to the
Choose Rotor screen.

Operation

How to use the FUNCTION field

2-52 WX Ultra Series Thermo Scientific

4 To select a desired rotor from the

“Rotor Management”:
(1') Select “Rotor Management”.

(2') Select a desired rotor using the
upward or downward cursor key

.

(When using fixed angle rotor T-8100)

(3’) Select “Yes”.

>> The RCF value of the running rotor is displayed in the RCF display field.

• The display switches to the
Rotor Management screen
and the Rotor List is
displayed.

• The display switches back
to the Run Screen and the
selected rotor type is
displayed in the message
display field of the bottom
row.

RFC display field

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-53

How to set an RCF value

Enter a rotor number and an RCF value, and the machine will calculate, set, and display the speed.
Shown below is the procedure.

Step Key operation Screen display and considerations

1 While in the Run Screen, set a speed. >> See Section Setting run conditions.

2 (1) While in the Run Screen, use cursor

keys to move the cursor to RCF • ω2T
and press ENTER key.

(2) Move the cursor to RCF

max

(or

RCF

avg

) and press ENTER key.

3 Check that ROTOR LIST blinks and

press the ENTER key.

• The FUNCTION field
switches to what is shown
in the diagram to the left.

•The RCF

max

field blinks.

•The FUNCTION field
switches to the RCF field.

• The ROTOR LIST field
blinks.

• The display switches to the
Choose Rotor screen.

Operation

How to use the FUNCTION field

2-54 WX Ultra Series Thermo Scientific

4 Select a desired rotor.

To select a desired rotor from the
“Used Rotor List”:
(1) Enter No. that is found in the Rotor
List.

To select a desired rotor from the
“Rotor Catalog”:
(1)' Select “Rotor Catalog”.

(2)' Select Fixed Angle Rotors in the
rotor types.
(When using fixed angle rotor T-890)

(3)' Select fixed angle rotor T-890.

(4') After checking the specifications of
the rotor, select “Yes”.

>> When you select “Yes”

>> The RCF value corresponding to the currently set speed is displayed in the RCF
setting field.
(--- --- --- --- --- ---: Displayed at the initial time of no setting.)
>> When you select “No”, the display goes to the same as the display of process
4 (2'). Perform setting again.

• The display switches to the
Used Rotor List screen.

• You can search for the rotor
number you need in the order
starting with the latest
date/time.

• The display switches to the Rotor

List screen.

• The angle rotor list is displayed.

• The specifications of the T-890
rotor are displayed.

• The display switches back to
the Run Screen and the selected
rotor type is displayed in the
message display field of the
bottom row.

RCF display field

RCF setting field

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-55

4 To select a desired rotor from the

“Rotor Management”:
(1) Select “Rotor Management”.

(2) Select a desired rotor using the
upward or downward cursor key

.

(When using fixed angle rotor T-8100)

(3) Select “Yes”.

>> The RCF value corresponding to the currently set speed is displayed in the RCF
setting field.
(--- --- --- --- --- ---: Displayed at the initial time of no setting.)

5 Use cursor keys to move the cursor to

the RCF setting field, and enter a
desired centrifugal force.
Example: RCFmaX = 5000 x 100g

• Specify the RCF value to hundreds.
If you make no input for 30 seconds, the cursor will disappear, and you will be
unable to make an input. At that time, press a cursor key.

• The display switches to the
Rotor Management screen and
the Rotor List is displayed.

• The display switches back to
the Run Screen and the selected
rotor type is displayed in the
message display field of the
bottom row.

RCF display field

RCF setting field

• The value you have just entered
appears in the RCF setting
field.

• Based on the rotor and RCF
value, the system automatically
calculates the speed and
displays it in the SPEED
display field.

Operation

How to use the FUNCTION field

2-56 WX Ultra Series Thermo Scientific

Setting ω2T

This machine is provided with a function to perform an ω2T run. To perform an ω2T run, set the ω2T value
instead of the run time.

Note

1. Press ESC key to cancel the RCF field.

2. When you run this centrifuge at a speed determined based on an RCF value, a slight rounding
may occur (of up to 2%) between the set RCF value and the actual value, because the speed
setting is set in increments of 100 rpm.

Speed (rpm)

Tim e

ω2T

[ω

2

T run]

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-57

Step Key operation Screen display and considerations

1 Set the Speed in the Run Screen. >> See Section Setting run conditions.

2 (1) Use cursor keys to move the cursor

to RCF • w

2

T on the Run Screen and

press the ENTER key.

(2) Use cursor keys to move the cursor

to w

2

T and press the ENTER key.

3 Use cursor keys to move the cursor to

the w

2

T setting field and enter a

desired w

2

T value.

Example: 9.87 x 1011

4 When you press START key, the

machine starts to perform an w

2

T run.

• The contents of the display
in the FUNCTION field
change.

• ω

2

T blinks.

• The FUNCTION field
switches to the ω

2

T field.

ω2T display field

ω2T setting field

• The value you entered is
displayed in the ω

2

T setting

field.

• The time value is calculated
from the speed and the ω

2

T

value and the run time is
displayed in the Time Setting
field.

• Arithmetic and integral
operations are performed in
the ω

2

T display field.

ω2T display field

Operation

How to use the FUNCTION field

2-58 WX Ultra Series Thermo Scientific

Defrost function

If the chamber door is left open between low temperature runs, moisture from the ambient air
humidity can condense (and even freeze) on the cold evaporator chamber walls. The potential for
condensation increases as the ambient air temperature and humidity increases. If a run is performed
with a significant amount of moisture in the evaporator chamber, the length of time required to reach a
high vacuum will be increased.

To minimize condensation, when the evaporator chamber is cold, we recommend that you keep the
chamber door closed as much as possible. If moisture does accumulate, before performing the next run,
we recommend that you wipe the chamber dry. For situations where condensation has frozen to the
chamber walls, a defrost function is provided as a convenient means to melt ice buildup so that the
moisture can then be removed.

How to use the defrost function:

Defrost operation will heat the evaporator gradually and after the lapse of 10 minutes, the defrost
operation and vacuum ends automatically. (After the end of the defrost operation, the DEF display in
the message field disappears.) During the defrost operation, DEF in the message display field in the
bottom row on the Run Screen blinks. To abort or end the Defrost Function, highlight DEF and press
ENTER. To turn off vacuum, press vacuum key.

Note When the ω

2

T display field reaches the set value of ω2T, the machine decelerates and stops.

During deceleration, the

ω2T display field continues to accumulate value until the rotor stops and

the run ends.

Step Key operation Screen display and considerations

1 Use cursor keys to move the cursor to

DEF on the Run Screen.

2 With DEF highlighted, press the ENTER

key.

• Use cursor keys to highlight
the DEF function.

• DEF blinks in the message
field to notify that a defrost
operation and vacuum has
started.

• Vacuum indicators will also be
displayed.

Operation

How to use the FUNCTION field

Thermo Scientific WX Ultra Series 2-59

Setting and resetting zonal operation

Selection between normal operation and zonal operation is performed by special operations using the
FUNCTION field on the Run Screen.

1. Setting zonal operation

Note

1. Before starting defrost operations, wipe away any moisture and close the door.

2. The temperature rise of the rotor due to defrost operations (for 10 minutes) should be 1 C or
less.

3. During rotor acceleration or at set speed, defrost can be set but the defrost function is not
available.

4. If DEF is selected during accel or set speed, it will not function until the run times out or stop
is pressed.

Step Key operation Screen display and considerations

1 On the Run Screen, use cursor keys to

move the cursor to ZONAL in the
FUNCTION field, and press

or

• ZONAL is displayed in the
message field so that the
machine is ready to start
zonal operation.

Operation

Rotor management

2-60 WX Ultra Series Thermo Scientific

2. Resetting zonal operation

Rotor management

It is very important to manage the life of each rotor you use with the ultracentrifuge. The warranted life
of the rotor is different depending on the type of rotor, the material the rotor is fabricated from and the
policy of the manufacturer of the rotor. The warranted life of a Thermo Ultracentrifuge rotor is defined
by a maximum number of runs. Other manufacturers use a combination of either the maximum
number of runs or a maximum number of hours to limit the warranted life of the rotor.

For some rotors, there exists a primary and a secondary life. The primary life is defined as the initial
usage at speeds up to the maximum rated speed of the rotor. Once this primary life is expended, the
rotor has a secondary life at speeds up to a derated maximum speed of the rotor-typically 90% of the
initial maximum rated speed.

Rotor life management

In order to comply with warranty requirements, rotor use must be documented in a rotor log book
provided with the ultracentrifuge.

Additionally, the Sorvall WX Ultra series ultracentrifuges have the capability to keep track of the
number of runs and hours of use for each rotor. This information can be used to automatically notify
the user of the need for deration at the end of the primary life and to preclude the use of rotors once
their warranted life as defined by these parameters have come to an end.

Step Key operation Screen display and considerations

1 On the Run Screen, use cursor keys to

move the cursor to ZONAL in the
FUNCTION field, and press

or

• ZONAL in the message
field disappears and the
machine status switches to
normal operation.

Note If no key operation is performed for 30 seconds, the cursor disappears and any entry is
disabled. At that time, press the cursor key to cause the cursor to blink once again.

Operation

Rotor management

Thermo Scientific WX Ultra Series 2-61

To utilize this feature, each time a rotor is used it must be recorded by the ultracentrifuge. To do this, a
rotor must be initially registered in the Rotor Management software. Each time the rotor is used in the
Sorvall WX Ultra series ultracentrifuge, it must be selected from the Rotor Management Screen prior
to starting the run. Additionally, should the rotor be used in other ultracentrifuges, the Rotor
Management information can be edited to record this usage. Rotors may also be deleted from the Rotor
Management Screen if they are no longer to be used.

If you use the rotor beyond its maximum permitted life, a serious accident may occur. (For more
information, see rotor instruction manual.)

Automatic rotor deration

You can register a rotor in the ultracentrifuge before use. After the rotor has been registered, you can
utilize the rotor management feature of the ultracentrifuge each time a run is made using the rotor.
When the registered rotor comes close to the end of its life, the ultracentrifuge indicates it by displaying
a message on the screen. When the rotor reaches its primary life, the ultracentrifuge derates its
maximum permitted speed and inserts [] at the right side of the rotor S/N. Once the maximum
permitted speed is derated, the actual speed of the rotor is automatically restricted within the new
limits.

Automatic rotor exclusion

When a registered rotor reaches the end of its life, the ultracentrifuge indicates it by displaying a
message on the screen. Subsequent runs of this registered rotor are precluded.

Registering a rotor

A standard rotor can be registered when you use the rotor management feature of the ultracentrifuge. If
the rotor has not been registered, the feature does not operate for the rotor. To register a rotor, use the
procedure described below.

As an example, suppose that you need to register a fixed angle rotor with the
following data, and that it has been used with an ultracentrifuge of a different
model from the one mentioned below:
Model: T-890 Serial number: 9910876
Total number of runs thus far made: 500
Total number of hours thus far spent in the runs: 1200 hours, 24 minutes

Note The rotor life management data stored in the ultracentrifuge is not covered by the warranty.
When a rotor is used with the ultracentrifuge, be sure to record the rotor management data in the
rotor log book. (You can also paste a printout in the log book.) If the rotor log book is not
maintained correctly, the rotor will not be warranted.

disk

Operation

Rotor management

2-62 WX Ultra Series Thermo Scientific

Step Key operation Screen display and considerations

1 While in the Run Screen, use the cur-

sor keys to move the cursor to RLM
and press ENTER key.

Switch each page of the Rotor List by
using the left and right arrow keys

().

2

Use the cursor keys ( and ) to

move the cursor to the line that is not
yet registered (New Rotor), and press
the ENTER key.

3 Select “Creating or Changing”.

4 Select “Fixed Angle Rotor” in the Rotor

List screen.

and

• The display switches to the
Rotor Management screen
and the Rotor List is
displayed.

• The display switches to the
Rotor List screen.

• The display switches to the
Fixed Angle Rotor List
screen

Operation

Rotor management

Thermo Scientific WX Ultra Series 2-63

5 Select “T-890” rotor.

6 After checking the specifications of the

rotor, enter “Yes”.

7 As the cursor has been moved to the

S/N field, enter serial No. of the rotor
by using numeric keys.
(Do not press the ENTER key.)

Registration of an unused rotor is fin­ished by pressing the ENTER key.

8 Enter the total number of runs in the

Runs field. (Do not press the ENTER
key.)

9 Enter the total number of hours in the

Hours field by using numeric keys. (Do
not press the ENTER key.)
Note: Enter this running time up to one
decimal place in units of hour.
1200 hours 24 minutes ---> 1200.4

10 Press the ENTER key.

• Specifications of the T-890
rotor are displayed.

• The display switches to the
Rotor Management screen.

• You can enter a serial
number of the rotor.

• The cursor moves to the
Runs field. You can enter the
total number of runs.

• The cursor moves to the
Hours field. You can enter
the number of hours.

Operation

Rotor management

2-64 WX Ultra Series Thermo Scientific

Thus the rotor has been registered.

Deleting a registered rotor

If a registered rotor becomes unnecessary, you can delete it from ultracentrifuge memory.

As an example, suppose that you need to delete the T-890 fixed angle rotor registered in the previous
section.

Note When the ENTER key is pressed in the above steps “7” to “9”, “0” is registered as a numeric
value in each step. When the serial number must be corrected, the rotor must be deleted once and
the value must be reentered from step “1”. (See the next page.)
In the steps “7” to “9”, it is possible to change the value repeatedly by moving the cursor with the
arrow keys unless the ENTER key is pressed.

Note Once a registered rotor is deleted, all of the life management data for the rotor will be lost.

Step Key operation Screen display and considerations

1 While in the Run Screen, use the cur-

sor keys to move the cursor to RLM
and press ENTER key.

2 Use cursor keys to move the cursor to

the T-890 line to be deleted and press
the ENTER key.

3 Select “Deleting” by pressing the fol-

lowing keys.

4 Select “Yes” by pressing the following

keys.

>> If you select “No”, you will get the display you see in process 2.

• The display switches to the
Rotor Management screen.

Operation

Rotor management

Thermo Scientific WX Ultra Series 2-65

Updating the existing data for a rotor

When a rotor is registered in the Sorvall WX Ultra series ultracentrifuges without a Rotor Management
function, both Total number of runs and Total number of hours can be updated. The updated value is
accepted only when it is greater than the registered value. It must not be smaller than the registered
value.

To update the existing life management data for a standard rotor, perform the following procedure:

Step Key operation Screen display and considerations

1 While in the Run Screen, use the cur-

sor keys to move the cursor to RLM
and press ENTER key.

2 Use cursor keys to move the cursor to

the T-890 line to be updated and press
the ENTER key.

3 Select “Creating and Changing” by

pressing the following keys.

4 Enter the updated total number of runs

and the total number of hours by using
numeric keys.
Total number of runs

500.0 ---> 505.0
Total number of hours

1200.4 ---> 1210.4

• The display switches to the
Rotor Management screen.

• In the Runs field, the cursor
waits for an input.

Operation

When power failure occurs

2-66 WX Ultra Series Thermo Scientific

Thus, the current total number of runs and hours have been updated.

When power failure occurs

1. Rotation of the rotor

The rotor coasts to a stop. If the power is restored when the current speed is more than 500 rpm, the
rotor will be automatically accelerated to the set speed. If it is restored when the current speed is less
than 500 rpm, the rotor will decelerate to a stop.

2. Displays on the panel

All displays on the panel go out. The set values are all backed up by battery during the power failure.
When the power is restored, the displays will come on as before the power failure; the set values will be
restored; and the message “Power failure” will appear in the message display.

3. Removing the rotor from the ultracentrifuge

First open the chamber door, then remove the rotor according to the following procedure:
(1) Check that the rotor is at rest. Listen carefully for any sounds coming from the drive.

(2) Then unplug the power cord from the wall outlet, and wait at least three minutes to avoid the risk
of electrical shock.

(3) Remove the four M5 hexagon head bolts fixing the front cover from both sides and pull down the
front cover by pulling its lower side forward. Then, remove the front cover.

The upper side of the front cover is an insertion type but not a screw fixing type.

DANGER Before removing the cover, top deck, or other components. for maintenance, be
sure to turn off the POWER switch of the centrifuge. Then unplug the power cord from
the wall outlet, and wait at least three minutes to avoid the risk of electrical shock.

WARNING

1. Never open the door during rotation.

2. Never touch the rotor during rotation.

CAUTION Never conduct operations in a manner other than as described in this operation
manual.

WARNING It can take more than three hours for the rotor to come to a complete stop
because the rotor chamber is under vacuum.
Before opening the door, wait until the rotor comes to a stop.

Operation

When power failure occurs

Thermo Scientific WX Ultra Series 2-67

Figure 2-16 Removing the front cover

(4) Open the air vent (by turning the vacuum release screw on the left of the vacuum chamber
counterclockwise) to let air into the rotor chamber. When the pressure in the rotor chamber reaches
atmospheric pressure, do not forget to tighten the vacuum release screw as it was before. (See Fig.
2-16.)

(5) Pull the door unlock wire on the right of the vacuum chamber and, at the same time, push the door
handle. Then the door opens. When opening the door, confirm that the rotor is not rotating. If it is
still rotating, immediately close the door.

(6) Remove the rotor. After the removal, close the air vent and put the front cover back to the
ultracentrifuge. Insert the top surface of the front cover in the bottom surface of the front side of the
table and put the bottom surface of the front cover on the support plate of the front cover by reversing
the removing procedure. Attach it on the frame with four M5 hexagon head bolts.

Tab le

Front cover

Cabinet cover

M5 hexagon head
bolt for fixing the
front cover (4 pcs.)

WARNING Never touch the rotor while it is rotating.

Operation

When power failure occurs

2-68 WX Ultra Series Thermo Scientific

Figure 2-17 Ultracentrifuge with the front cover removed

Figure 2-18 Installing the front cover

(7) Turn on the power breaker.

Handle

Vacuum release
screw

Vacuum
chamber

Door unlock wire

Power breaker

M5 hexagon head bolt for
fixing the front cover (4
pcs.)

WARNING When the centrifuge will not be used for a long time, keep the power breaker

off.

Operation

Features of the menu screen

Thermo Scientific WX Ultra Series 2-69

Features of the menu screen

Press MENU key on the key board, and the Menu Screen will appear. This feature is designed to allow
you to use the Sorvall WX Ultra series with additional options including:
(1) Centrifuge scheduler
(2) User list
(3) Alarm information
(4) Rotor catalog
(5) User customization routines
Key in the number of the item you wish to use and
press the ENTER key, and the respective screen will
appear.

Figure 2-19

Menu screen

Centrifuge scheduler

Scheduled centrifuge operation can be reserved.

1. Reservation of scheduled centrifuge operation

Step Key operation Screen display and considerations

1 On the Menu Screen, select a centri-

fuge operation schedule.

2 Use cursor keys to move the cursor to

No. you desire. (In this example, No.3
is selected as new reservation.)

• The Menu Screen switches
to the Centrifuge
Reservation screen.

• The ID Code input field is
displayed in the message

field of the bottom row.

Operation

Features of the menu screen

2-70 WX Ultra Series Thermo Scientific

2. Deleting the centrifuge reservation

You can cancel the operation schedule that is already reserved. This cancellation of reservation is
limited to the reserving person proper (with the same ID Code).
The reserved operation schedule will be deleted from the centrifuge reservation list after the lapse of the
scheduled date/time regardless of operation/non-operation of the centrifuge.

3 Enter an ID Code that is already regis-

tered. (4-digit number)

4 Select “Reserve”.

5 Use cursor keys to move the cursor to

the position at which you want to
change the date/time of reservation,
and enter a number by using numeric
keys.

Note Enter a time later than the current time.

6 At the desired date/time of reserva-

tion, press the ENTER key.

ID code

•The User Name
corresponding to the ID
Code is displayed. (In this
example, ABC lab. is
displayed.)

• The current date/time is
displayed at the reservation
time of the specified
reservation No.3.

Step Key operation Screen display and considerations

1 On the Menu Screen, select a centri-

fuge operation schedule.

• The Menu Screen switches
to the Centrifuge
Reservation screen.

Operation

Features of the menu screen

Thermo Scientific WX Ultra Series 2-71

User list

The Centrifuge User List screen is displayed. This screen allows you to register, change, and delete any
ID code and user name.

2 Use cursor keys to move the cursor to

reservation No. you want to delete. (In
this example, No. 3 is selected.)

3 Enter the ID Code of the reserving per-

son proper. (4-digit number)

4 Select “Delete”.

5 Select “Yes”.

• The ID Code input field is
displayed in the message
field in the bottom row.

ID code

•When the ID Code is
accepted, the reserve/delete
selection message is
displayed in the message
field of the bottom row.

• The confirmation message is
displayed in the bottom row.

• The No.3 reservation display
disappears from the
centrifuge reservation list
and the reservation delete
completion message is
displayed in the bottom row.

Operation

Features of the menu screen

2-72 WX Ultra Series Thermo Scientific

• ID code and user name

• Using example of ID code and user name

If ID Code is used when the centrifuge is operated:

1. The user can be known on the screen when the centrifuge is operated.

2. The user record is left in the centrifuge and the usage status for each user can be printed (printer:
optional).

3. The User Name can be attached to the user's original programmed operation and the user's own
program memory can be checked.

4. Reservation can be attained by the operation schedule table with the User Name attached.

1. Registration of user name

ID Code Password comprised of a 4-digit number. This number is not displayed

when the centrifuge is operated. The mark * is displayed instead of the
number.

User Name Character string of 8 digits or less. The user name corresponding to the

ID Code is displayed on the screen. Alphabetical upper-case and
lower-case characters and several types of special characters are
available.

Note Registration of ID Code and User Name is not always required for operation.
If the user does not need to be identified, the centrifuge can be operated without entering ID Code
and User Name.

Step Key operation Screen display and considerations

1

Select “User List” on the Menu Screen.

2 Use cursor keys to move the cursor and

press the ENTER key.
(For registration, select No.9.)

• The Menu Screen switches
to the User List Screen.

• For registration select
unregistered number.

• The inquiry message to ask
what to do in the message
field is displayed.

Operation

Features of the menu screen

Thermo Scientific WX Ultra Series 2-73

3 Select “Creating”.

4 Use cursor keys to highlight a charac-

ter and press the ENTER key to select
it.

5 After completion of the entry of char-

acters, use cursor keys to move the
cursor to SET and press the ENTER key
to register the character string.

6 Select “Yes”.

7 Enter the ID Code (4 digits) by using

numeric keys and press the ENTER key.

v

• The User List screen switches
to the User Name screen.

•Screen for entry of characters

• The cursor is positioned at
SET.

• The confirmation message is
displayed.

• The ID Code input field is
displayed in the message
field of the bottom row.

ID code

• Enter the ID Code and press
the ENTER key, and the
date of registration will be
displayed.

• The mark * is displayed
instead of the ID Code on
the screen.

Operation

Features of the menu screen

2-74 WX Ultra Series Thermo Scientific

2. Change of User Name

Note Changing a User Name is limited to the person (with the same ID Code) who registered it
and wants to change it.

Step Key operation Screen display and considerations

1 On the Menu Screen, select “User

List”.

2 Use cursor keys to move the cursor and

press the ENTER key. (Select No.9.)

3

Select “Changing”.

4 Enter the ID Code used for registering

No.9 by using numeric keys.

5 Change the User Name according to

the indication given on the screen.

• The Menu Screen switches
to the User List screen.

• For a change, select a
registered No.

• The inquiry message to ask
what to do in the message
field of the bottom row is
displayed.

• The ID Code input field is
displayed in the User List
screen.

• The User List screen switches
to the User Name
Registration screen.

• Character string input status

Operation

Features of the menu screen

Thermo Scientific WX Ultra Series 2-75

3. Deleting a user name setting

6 After entering characters, use cursor

keys to move the cursor to SET and
press the ENTER key to register the
character string.

7 Select “Yes”.

• The cursor is positioned at
SET.

• The confirmation message is
displayed.

• The changed User Name is
displayed together with its
date of registration on the
User List screen.

Note Deleting a User Name is limited to the person (with the same ID Code) who registered it.

Step Key operation Screen display and considerations

1

On the Menu Screen, select “User List”.

2 Use cursor keys to move the cursor to

No.9 and press the ENTER key. (The
User Name and ID Code of No.9 are
deleted.)

3 Select “Deleting”.

• The Menu Screen switches
to the User List screen.

• The inquiry message to ask
what to do is displayed in the
message field.

• The ID code input field is
displayed.

Operation

Features of the menu screen

2-76 WX Ultra Series Thermo Scientific

Alarm information

The Alarm Information screen displays the contents of the alarm signals and what to do when such
signals occur. If an alarm signal occurs while you are using this centrifuge, you can use this screen to
take immediate action.

For details, see Section 4 Troubleshooting.

Figure 2-20 Alarm Information screen

4 Enter the ID Code used for registering

No.9 by using numeric keys.

5 Select “Yes”.

>> When you select “No.” the display switches back to the screen display of Step

2.

6 Press the ESC key. >> The User List screen switches to the Menu Screen.

• The confirmation message is
displayed.

• The User Name and ID
Code of No.9 disappear.

Operation

Features of the menu screen

Thermo Scientific WX Ultra Series 2-77

Rotor catalog

You can refer to rotor types and rotor specifications that are available.

User Customizations

You can specify the language in which displays are given, set the current time, adjust screen contrast,
and make other settings, all of which enable you to easily run the centrifuge.

1. Instrument identification number

This function is intended to enter a centrifuge identification number (ID number).
You can enter a number of up to 8 digits. The centrifuge identification number is printed when the
machine log is output to the printer (printer: optional).
The same identification number is also used as the centrifuge identification number for managing log
data by using the operation log management program (option) on a PC.
The identification number is not factory-set. Unless you need it specially, use it as it is.

Step Key operation Screen display and considerations

1 On the Menu Screen, select “Rotor

Catalog”.

2 To refer to the fixed angle rotor

P100AT2, select “Fixed Angle Rotors”
on the Rotor List screen.

3 Select the “T-8100” rotor.

4 Press the ESC key several times to go

back to the Run Screen.

>> The Rotor Specification screen switches to the Run Screen.

• The display switches to the
Rotor List screen.

• The display switches to the
Fixed Angle Rotor List
screen.

• Specifications of the T-8100
rotor is displayed.

Operation

Features of the menu screen

2-78 WX Ultra Series Thermo Scientific

Figure 2-21 User Customizations screen

Figure 2-22 Screen Utilities screen

2. Screen Utilities settings

1. Display language and select Japanese or English.
Key in the number of the desired language and press ENTER key.

2. Changing from the Run Screen
>> Normal: Displays the Run Screen.
>> Graphics and zoom:
Twenty seconds after the actual speed reaches the set speed while in the Run Screen, the display will
automatically switch to the screen shown in Fig. 2-23 or 2-24.
The display will then return to the Run Screen if you press any key on the keyboard or while a
deceleration shift is in progress.

Figure 2-23

Graphics screen

Operation

Features of the menu screen

Thermo Scientific WX Ultra Series 2-79

Figure 2-24 Zoom screen

3. Changing date and time
Use this option to precisely set the current time setting of the internal clock.
Set an exact date and time for RTC operation.
Use cursor keys and numeric keys and then press ENTER to make a setting.

4. Screen contrast
Use cursor keys to adjust the contrast.

Figure 2-25

Contrast adjustment

3. Setting run parameters

(1) Run Time display
This function selects either “Time elapsed” or “Remaining time” as the time display in the run status.
When you select “Time elapsed”, “lapse” is displayed in the time display frame on the screen.
When you select “Remaining time”, “rem.” is displayed in the same frame.

Figure 2-26

Run Condition setting screen

Note

>> To get back to the Run Screen, press ESC several times.
>> If you make no entry for 30 seconds, the cursor will disappear, and you will be unable to make a
further input. If this happens, press a cursor key.

and

Low contrast

High contrast

Operation

Features of the menu screen

2-80 WX Ultra Series Thermo Scientific

(2) Run Time range
This function selects one of the following two items as the running time setting range in the normal
operation mode or zonal operation mode:
>> Accel + Run
Time from pressing the START key to a start of deceleration: T1 (ordinary running time).

Figure 2-27

Run time range setting

(3) Start mode select
This function allows you to select one of the following values as the degree of vacuum in the process
from the vacuum wait to accelerationto the set speed:
>> Medium vacuum: Approx. 133 Pa (ordinary)
>> High vacuum: Approx. 13 Pa

Figure 2-28

Vacuum waiting operation mode

Note The vacuum waiting time is not counted in the normal operation. To eliminate the run time
difference in the high vacuum start mode, vacuum the rotor chamber up to a high degree during
the rotor stop and then start the operation.

>> Run
Time from reaching to the set speed to a start of deceleration: T2

Speed

Speed

T1

T2

T1

T2

Tim e

Tim e

[Normal operation]

[Zonal operation]

Speed (rpm)

4,000

Operation

Print utilities (option)

Thermo Scientific WX Ultra Series 2-81

(4) Zonal speed
This function can change (in units of 100 rpm) the zonal speed in the range of 2,000 to 3,000 rpm.
For the zonal speed, see Section Zonal operation)

Figure 2-29

Run condition setting screen

4. Select option unit

This function allows you to set one of the following optional units through the RS232C.

1) Printer

2) Compass Centrifugation Software

3) Unused

Figure 2-30

Optional Unit connection screen

Print utilities (option)

This submenu option is used to print the various information maintained in the ultracentrifuge on the
optional printer. The information includes run records, rotor usages, memory contents, etc. When you
select “5. PRINT UTILITIES” from the “user customization routines” submenu, this submenu option
starts working and displays the PRINT UTILITIES screen.

Note When “Unused” is selected in the optional unit setting, machine log data will be deleted in
the order starting with the oldest data and new data will be stored in the memory if the machine log
data exceeds the memory area capacity of the centrifuge. For managing such machine log data, be
sure to set an optional unit. When such an optional unit has been set, the printer printout or PC
data input request message is displayed if the machine log data exceeds the memory area capacity.

Operation

Print utilities (option)

2-82 WX Ultra Series Thermo Scientific

Figure 2-31 PRINT UTILITIES Screen

Operating procedure

Note

• Select “1. Printer” of “4. Select Option Unit” in the User Customization Routines screen. Refer
to Section User Customizations.

No. Item Operating procedure

1 Machine Log

(1) Select the corresponding item number on the Print Utilities Menu screen.

2 Rotor Management

3 Rotor Log (1) On the Print Utilities Menu screen, select “3. Rotor Log”.

(2) On the Rotor Management screen, move the cursor to select a rotor and press the ENTER
key.

(3) Select “1: Yes” as print permission.

4 Set run parameters (1) Select the corresponding item number on the Print Utilities menu screen.

5 Run result

6 Set run parameters and result

• The display switches to the Rotor

Management screen.

Operation

Print utilities (option)

Thermo Scientific WX Ultra Series 2-83

7 Print program (1) On the Print Utilities menu screen, select “7. Print Program”.

(2) Select a program No. to be printed.

8 User log (1) On the Print Utilities Menu screen, select “8. User Log”.

(2) Move the cursor to select a User Name and perform printing.

9 Machine Management (1) On the Print Utilities menu screen, select “9. Machine Management”.

10 Record after each run (1) On the Print Utilities menu screen, select the corresponding item number.

(2) Move the cursor and select a setting condition.

11 Printout after each run

• The display switches to the
Program Map screen.

• The display switches to the
User List screen.

Operation

Print utilities (option)

2-84 WX Ultra Series Thermo Scientific

Contents of print utilities

No. Item Example printout

1 CENTRIFUGE RUN RECORDS

>> Ultracentrifuge run records for a maximum of 40 runs can be
printed out at a time.
>> Once the ultracentrifuge run records are printed out, they are
gone. Therefore, if necessary, save the produced printout for
future reference.

2 RUN RECORDS OF ALL ROTORS

>> Run records of all the rotors registered are printed out.
>> When a rotor reaches its primary life, an asterisk ([*]) is dis­played at the right of the maximum speed and the maximum
rated speed is derated by 10%.