Sorvall RC-28S User manual

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OPERATING INSTRUCTIONS

SORVALU RC-28S

SUPRASPEED

CENTRIFUGE

Du Pont Company Sorvall® Instruments Wilmington, Delaware 19898 U.S.A.

Sorvall Centrifuges

PN 59154-1 Issued August 1989

Sorvall® Centrifuges

This manual is a guide for use of the

SORVALL® RC-28S Supraspeed® Centrifuge

Data herein has been verified and validated and is believed adequate for the intended use of the centrifuge. If the centrifuge or procedures are used for purposes over and above the capabilities specified herein, confirmation of their validity and suitability should be obtained, otherwise Du Pont does not guarantee results and assumes no obligation or liability. This publication is not a license to operate under, nor a recommendation to infringe upon, any process patents.

NOTES,

CAUTIONS, and WARNINGS within the text of this manual are used to

emphasize important and critical instructions:

WARNING: A Warning informs the operator of a hazard or an unsafe practice that

could result in personal injury, affect the operator's health, or contaminate the environment.

CAUTION: A Caution informs the operator of an unsafe practice that could result in

damage of equipment.

NOTE:

1988 by E.I. du Pont de Nemours & Co.

A Note highlights essential information.

Sorvall® Centrifuges Safety

Important Safety Information

Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure safe operation of this centrifuge, anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual.

WARNING

Use Sorvall® rotors only. Use of another manufacturer's rotor can cause rotor failure

which could result in personal injury and/or centrifuge damage. When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of

the materials and the hazards associated with them in the event leakage or rotor failure occurs during centrifugation, in which case neither the centrifuge nor the rotor can protect you from particles dispersed in the air. To protect additional precautions be taken to prevent exposure to these materials, e.g., use of controlled ventilation or isolation areas.

Always be aware of the possibility of contamination when using radioactive, toxic, or pathogenic materials. Take all necessary precautions and use appropriate decontamination procedures if exposure occurs.

yourself,

we recommend

Never use any material capable of producing flammable or explosive vapors. Never exceed the maximum rated speed of the installed rotor; to do so can cause rotor

failure.

Always reduce (derate) rotor speed whenever:

• the rotor speed/temperature combination exceeds the solubility of the gradient material and would cause it to precipitate;

• rotor compartment load exceeds the maximum allowable compartment load specified (check this whenever average fluid density is greater than 1.2 g/ml). See Chapter 4, Operation.

Failure to reduce rotor speed under these conditions can cause rotor failure.

CAUTION

Do not run or precool a rotor at the critical speed, as this will have a detrimental effect on centrifuge component life. See Table 4-1, Rotor Information.

Do not operate centrifuge with a rotor out of balance. To do so can cause damage to the centrifuge drive assembly.

Do not operate centrifuge without rotor properly installed: rotor cover must be on and locked in place, and the rotor locked to the centrifuge drive. See rotor instruction manual.

Locate the centrifuge on a level floor to avoid rotor imbalance during operation.

The centrifuge can be damaged if connected to the wrong voltage. Check the voltage before plugging the centrifuge into a power source. Du Pont is not responsible for incorrect installation.

Always maintain the centrifuge in the recommended manner. See Chapter 7, Maintenance.

iii/iv

Sorvall® Centrifuges Table of Contents

Table of Contents

Page

Safety Information iii

Chapter 1. DESCRIPTION

General Description 1-1 Centrifuge Specifications 1-2 Centrifuge Features 1-3 System Descriptions 1-5

Chapter 2. INSTALLATION

Inspection 2-1 Location 2-1

Ambient Temperature 2-2

Leveling 2-2 Electrical 2-2

Chapter 3. CONTROLS

Control Panel 3-1 PROGRAM Key Functions & Display Screens 3-3 CALCULATE Key Functions & Display Screens 3-9 DIAGNOSTICS Key Functions & Display Screens 3-9

Chapter 4. OPERATION

Understanding the Centrifuge Controls 4-1 Turning the Centrifuge On 4-1 Normal Run 4-1 Slow Start/Slow Stop 4-3 RCF Run 4-5 Key#Code 4-6

Zonal Run 4-7 Hold Run 4-8 fctfdt Run 4-9 Programmed Run 4-10 Control Panel Lock 4-11 Precool/Preheat 4-12 Reducing Speed for Rotor Compartment Loads in

Excess of Design Mass 4-12

Table of Contents Sorvall® Centrifuges

Table of Contents (continued)

Page

Chapter 5. CALCULATOR

Calculator Display Screens 5-1 Calculate Speed/RCF for Rotor Time/fo^dt 5-1 Calculate Speed/RCF at a Radius 5-2 Calculate K Factor 5-2 Calculate Rotor Conversion/Time 5-2

Chapter 6. TROUBLESHOOTING & DIAGNOSTICS

Display Messages 6-1 Diagnostics:

General 6-5 Screen 1, Faults 6-6 Screen 2 6-7

Screen 3 6-9 How to Assign or Change a Key# 6-9 How to Change Slow Start/Slow Stop Transition Speed 6-12

Chapter 7. MAINTENANCE

Maintenance Schedule 7-1 Cleaning:

Rotor Chamber 7-2

Tapered Drive Spindle 7-2

Cabinet 7-2

Control Panel 7-2 Door Seal 7-2 Refrigeration System Condenser 7-4

Air Louvers 7-4 Lubrication 7-4 Changing the Vacuum Pump Oil & Mist Eliminator Filter 7-4 Emergency Sample Recovery 7-8

Test of RUN Display Lights & Control Panel Indicators 7-10 Parts Ordering Information 7-11 Service Decontamination Policy 7-11

APPENDIX

Vacuum Pump Oil Specifications Warranty

Glossary Index Program Log Cards Decontamination Certificates

Sorvair Centrifuges

Table of Contents

List of Illustrations

Figure

1-1. SORVALL® RC-28S Supraspeed® Centrifuge viii

2-1.

Centrifuge Dimensions 2-1

2-2.

Leveling Adjustment 2-2

3-1.

Control Panel 3-2

7-1.

Door Seal Removal 7-3 7-2. Parts Identification, Vacuum Pump Oil Change 7-6 7-3.

Door Interlock Override 7-9

7-4. Vacuum Release Solenoid Valve Location 7-9

List of Tables

Table

4-1.

Rotor Information 4-4

6-1.

DIAGNOSTICS, Screen 2 Fields 6-7 6-2. DIAGNOSTICS, Screen 2 Operations 6-7 6-3.

DIAGNOSTICS, Screen 3 Fields 6-8

7-1.

Maintenance Schedule 7-1

Page

vii

Sorvall® Centrifuges

viii

Figure 1-1. SORVALL® RC-28S Supraspeed® Centrifuge

Sorvall®Centrifuges Description

Chapter 1

DESCRIPTION

This manual provides you with the information you need to install, operate and maintain your SORVALL® RC-28S Supraspeed® Centrifuge. If you encounter any problem concerning either operation or maintenance that is not covered in the manual, please telephone Du Pont for assistance. In the United States, call toll free

(800)551-2121.

SORVALL® Products.

General Description

The Sorvall® RC-28S Supraspeed® Centrifuge defines a new class of centrifuge that brings an unparalleled level of performance to centrifugation. Created from the latest

design technology, it incorporates usability, dependability, and safety with an extremely

versatile speed range to set a new standard in value. When used with a Sorvall®

Supraspeed® rotor, the RC-28S can reach a top speed of 28 000 rpm with a maximum g force of 100 095. At 100 095 x g you can perform applications in the RC-28S that previously required an ultracentrifuge. In addition to the Supraspeed® rotors designed for use with the RC-28S, the RC-28S accepts all Sorvall® superspeed rotors, increasing its usefulness by making it capable of a wide range of applications.

Outside the United States, contact the distributor or agent for

The centrifuge is microcomputer controlled and designed to be user friendly. It can be operated using basic parameters only or you can decide to use its special features. Because of this versatility and its user-friendliness, it can easily be operated by inexperienced persons learning centrifugation methods or by experienced persons experimenting with new methods.

The brushless dc drive which offers the optimum in centrifuge drive performance is one distinct feature of the RC-28S. Other features of the centrifuge are: automatic rotor recognition; programmability; RCF and for sample reorientation; overtemperature control; a control panel lock to prevent run parameters from being changed; and a built-in calculator that can be used to perform

centrifugation calculations, including conversions between RCF and speed, time and fco2dt or the calculation of K factors and run times when conversion of published run parameters is desired.

The centrifuge also has extensive diagnostic routines that allow the user to identify system malfunctions and perform maintenance and repair procedures.

For more on the features of the RC-28S, plus more on the centrifuge drive and other systems, read the table on the next two pages and the descriptive paragraphs that follow the table.

fco2dt

control; slow start and slow stop control

1-1

Description

Centrifuge Specifications

Son/all® Centrifuges

Rotor Management

SPIN-RIGHT® Rotor Management System

automatically recognizes installed Sorvall® rotor when chamber door is closed. Pre-

vents operation of rotors above their maxi-

mum speed. Primary function of the system

is to provide an added margin of safety during operation; however it is effective only when it is operated as intended. Any misuse of the system, as with any other centrifuge system or feature, could result in unsafe operation.

Rotor Speed

Set Speed:

from 100 rpm to 28 000 rpm in increments of 10

Speed Control:

±10 rpm from 0-2000 rpm;

±0.5 % above 2000 rpm.

Speed Displayed:

digital readout during a run of actual speed

Rotor Temperature

Set Temperature:

from -20°C to +40°C

Overtemperature:

automatic or settable from 0°C to 45°C

Temperature Control:

±10Cfrom2°Cto40°C

Temperature Displayed:

digital readout during a run of actual temperature in 1 ° increments

Time

Set Time:

one minute to 99 hours and 99 minutes; can select HOLD for

continuous operation

Time (continued)

Time Displayed:

digital readout indicates remaining time for a timed run or elapsed time for continuous run

Vacuum

Rotary vane pump system.

Chamber pressure:

< 15 millibars (1.5 kPa)

Ambient Temperature Range

from 15°Cto38°C

Dimensions:

Width: Height: 93 cm (36.5 in)

Depth:

Clearances:

15 cm (6 in) required on left side of centrifuge

70 cm (27.5 in)

to top deck

123 cm (48.5 in)

to top of control panel

103 cm (40.5 in)

Net Mass

385 kg (850 lbs)

Electrical Requirements

Can be supplied for use on the following single phase power sources

200 V, 60 Hz, 30 A 200 V, 50 Hz, 30 A 208 V, 60 Hz, 30 A3 220 V, 50 Hz, 30 A 230 V, 60 Hz, 30 A3 240 V, 50 Hz, 30 A

Receptacle:

power cord fits Hubbell receptacle No.

9330 or equivalent

Temperature range with power ON but no run in process (standby) is from -10°C to +30°C.

The centrifuge will operate at ambient temperatures up to 38°C, but refrigeration system performance will be less than optimal above 25°C.

CSA Certification applied for.

1-2

Sorvall® Centrifuges

Description

Centrifuge Features

High Performance Drive

A brushless dc direct drive system de-

signed for longer motor life and greater reliability; provides high torque; fast acceleration; and smooth, precise speed

control. No brushes means less maintenance. Controlled by dual microprocessors with accurate speed control during acceleration and a Positive Action Brake system that provides controlled braking from set speed all the way to 0 rpm. Includes Sorvall® Gyro-Action Centering System that permits "eye balancing" of tubes. Imbalance detector protects against damage from gross imbalance conditions. Accepts all Sorvall® Superspeed and Supraspeed® rotors.

Self-

Automatic Rotor Recognition

SPIN-RIGHT® Rotor Management System automatically recognizes installed

Sorvall® rotor when chamber door is closed. Provides optimal instrument

control. Prohibits operation of a rotor above its maximum speed. Primary function of the system is to provide an added margin of safety during operation; however, it is effective only when it is operated as intended. Any misuse of the system, as with any other centrifuge system or feature, can result in unsafe operation.

Vacuum

Allows Sorvall® Supraspeed® rotors to be

operated at speeds up to 28 000 rpm.

Automatically activated when required.

RCF Run

RCF can be chosen in place of a speed to control run. Allows RCF to be reproduced from run to run and rotor to rotor.

Accepts values up to 100 095 x g, de-

pending on the rotor used.

Slow Start / Slow Stop

Automatic control of acceleration and

deceleration to and from transition speed; provides optimal reorientation conditions which prevent disturbance of gradient or pellet. Optimal times to and from transition speed are automatically determined for identified rotor or can be manually selected; transition speed can even be changed if desired. Flexibility of control means you can duplicate a slow start/slow stop run done in any other centrifuge.

Zonal Run

Allows operation with the chamber door

open to dynamically load a Sorvall®

TZ-28 Zonal Rotor. Loading speed selection from 1300 rpm to 2500 rpm. A key number is required for zonal operation.

Precise Temperature Control

A high-capacity, highly efficient refrigeration system that combines heating and cooling. The combined heating and cooling provides temperature control from 2°C to 40°C and eliminates the need to defrost the chamber. Temperature sensor is mounted away from the chamber wall to further improve accuracy of rotor temperature measurement.

Automatic sample temperature protec-

tion; maximum temperature will be set automatically or can be entered manually. Convenient precooling of rotors.

fco2dt Run

A built-in integrator that provides exact run-to-run reproducibility by controlling the accumulating ing value is displayed during the run.

fo)2dt.

The accumulat-

Control Panel Lock

Locks the control panel to prevent run conditions from being changed without

the operator knowing. A key number is required to lock and unlock the control panel.

1-3

Description

Centrifuge Features

Sorvall® Centrifuges

Programmability

Records run settings in memory. Once recorded, programs can be recalled to allow duplication of run. Up to 20 programs can be recorded. A key number is required to save a program to pre-

vent unauthorized changes; however,

programs can be recalled without a key number.

Calculator

A built-in calculator that performs

basic centrifugation calculations: RCF, Speed, Time, leting time. Can be used to convert run conditions from rotor to rotor.

S(o2dU

K Factor, and pel-

Security Key Number

A four digit security number that is

required to access protected data and some diagnostics. Also required to activate Zonal, Program Save, and the Lock. Centrifuge is supplied with one factory-set number, but you can program multiple numbers for greater security and multiple users.

Advanced Diagnostics

Built-in diagnostic routines include several levels of electronic troubleshooting aids that allow you to diagnose problems if they occur. Also used to perform limited maintenance procedures and centrifuge updates.

Rotor Chamber

The stainless steel rotor chamber is

epoxy coated and free of wall sensors

making it durable and easy to clean.

Chamber seal is located in the door rather than in the top of the chamber; minimizes accumulation of dirt on the seal which improves the seal. The door seal can easily be removed for cleaning. The sliding chamber door retracts which makes the chamber easy to access and solves door clearance problems.

Size

The RC-28S is narrower than most superspeed and ultraspeed centrifuges. Requires less floor space.

Operator's Guide

Readily accessible operating instructions and aids that pull out from under the control panel. Includes convenient Program Log records and rotor specifications.

Serviceability & Technical Support

Extensive diagnostics and easy to access components make the RC-28S easy to service. Complete product literature available plus a toll free

number to call for technical assistance.

1-4

Sorvall®Centrifuges Description

System Descriptions

Drive System

The RC-28S drive system uses state-of-the-art brushless dc motor technology that

offers the optimum in centrifuge drive performance.

A brushless dc motor uses an electronic commutator in place of a mechanical

commutator, which eliminates brushes and the maintenance problems associated with them, plus it eliminates the speed and current limitations of a mechanical commutator. The high torque of the dc motor results in faster acceleration and braking, while the high efficiency means less power consumption and less heat. Less heat in the motor means that the drive runs cooler which eliminates the need for any auxiliary means of motor cooling, such as Freon® or water.

The motor used is inherently non-cogging which ensures the smoothest acceleration

and deceleration possible. Cogging is typical of induction and brush type motors and can cause sample disturbance during acceleration and deceleration.

The RC-28S drive system is controlled by dual microprocessors allowing extremely accurate speed control during acceleration and deceleration and eliminating overshoot of set speed. The accuracy of the speed control results in smoother slow start and slow stop,

plus positive action braking from set speed to 0 rpm. The microprocessors operate independent of each other to constantly monitor various centrifuge functions and, as an added safety factor, they maintain constant internal communication with each other to test and evaluate the performance of the other. The many tests that are performed routinely throughout operation determine if any preset limitations are exceeded; any failure detected will cause immediate shutdown by the processor detecting the problem.

In addition, the centrifuge will shut down if communication fails between the processors

or in the unlikely event both processors fail.

SPIN-RIGHT® Rotor Management System

The Sorvall® RC-28S SPIN-RIGHT® Rotor Management System automatically insures

optimal control of the selected speed and temperature parameters and prevents the

inadvertent setting of a speed beyond the capability of a given Sorvall® rotor. The Sorvall® rotor installed in the chamber is recognized through an ultrasonic scanning device that is activated when the chamber door is closed. The rotor identity is then displayed in the SET display of the control panel.

The primary function of the rotor management system is to provide an added margin of safety during operation. This system is effective only when it is operated as intended.

Any misuse of the system, as with any other centrifuge system or feature,can result in

unsafe operation

Refrigeration System

The refrigeration system consists of: a stainless steel evaporator that acts as the rotor

chamber, a 2-hp hermetically sealed compressor, an air-cooled condenser, and the

suction-capillary tube assembly. A hot gas bypass circuit allows for quiet, non-cycling

1-5

Description Sorvall®Centrifuges

compressor operation as well as improved temperature control and frost-free operation.

The refrigeration system is factory charged with Freon® 502. The desired run temperature is entered on the control panel and is maintained by the

system electronics which cycles the hot gas circuit as required. If desired, a maximum temperature can be entered on the control panel; if a value is not entered manually, the maximum temperature will default to +5°C above set temperature. Any failure causing the temperature to exceed the maximum setting will result in the termination of the run and a corresponding message will be displayed on the control panel to alert the operator.

Vacuum System

The vacuum system includes a direct drive vacuum pump, an air-inlet solenoid (bleed) valve, mist eliminator, the evaporator chamber, and interconnecting tubes and fittings. Reduced rotor chamber pressure is sensed by a Pirani convection gauge located at the pump inlet manifold and monitored by the machine control microprocessor. When activated, this system provides a vacuum level between 1 and 15 millibars.

The vacuum system is automatically activated only when the set speed is higher than the top air speed of one of the Sorvall® Supraspeed® rotors designed specifically for use with the RC-28S.

In the event of a vacuum loss or insufficient vacuum, a message will appear in the control panel SET display to notify the operator. In an emergency, such as a power failure, when sample recovery is desirable, the chamber door can be opened manually

(see Chapter 7 for procedure).

1-6

Sorvall® Centrifuges

Installation

Chapter 2

INSTALLATION

This chapter contains instructions to prepare your Sorvall® RC-28S for operation.

Inspection

When you receive your centrifuge, carefully inspect it for any signs of shipping

damage. If you find damage, please report it immediately to the transportation company and file a damage claim, then notify Du Pont.

Check the parts received with the centrifuge against the shipping list; if any parts

are missing, contact Du Pont (see back cover).

Location

Locate the centrifuge on a level floor. To permit free air circulation, the location must allow space for the size of the centrifuge (figure 2-1), plus an additional 15 cm (6 inch) space on the left side of the centrifuge. Free air circulation is very important for the centrifuge to function properly.

LEFTSIDE OF

CENTRIFUGE

POWER

CORD

200cm -

(78.5 in)

123 cm

(48.5 in)

Figure 2-1. Centrifuge Dimensions

2-1

Installation

Son/all® Centrifuges

Ambient Temperature

An ambient temperature range of 15°C to 38°C must be maintained. If the inlet air

temperature is above 25°C, the centrifuge will not maintain low temperatures at

high speeds.

Leveling

WARNING

The front stabilizers must be used as explained below to lift the casters off the

floor; this will help minimize centrifuge movement in the event of a rotor

failure.

When the centrifuge is situated at its operating location, lower and secure the front locking stabilizers. Lower the two locking stabilizers by hand until they are finger tight against the floor. Then use the 9/16 inch (14 mm) wrench supplied with the centrifuge to rotate each stabilizer an additional 2 1/2 turns. This will raise the front casters about 3 mm (1/8 inch) off the floor and level the centrifuge (based on level floor).

LOCKING STABILIZERS

(9/16 in [14 mm] HEX)

APPROX. 3mm (1/8 in)

OFF THE FLOOR

Figure 2-2. Leveling Adjustment

Electrical

Your centrifuge was ordered for a specific single phase power source (preferably regulated). The nameplate on the back panel of the centrifuge tells which one of the following classifications you ordered:

200 V, 60 Hz, 30 A 208 V, 60 Hz, 30 A* 230 V, 60 Hz, 30 A*

200 V, 50 Hz, 30 A 220 V, 50 Hz, 30 A 240 V, 50 Hz, 30 A

If the line voltage varies ±10% of its nominal value, you may notice variations in the performance of the centrifuge. If the line voltage exceeds this tolerance, it may damage the centrifuge.

* CSA Certification applied for.

2-2

Sorvall® Centrifuges installation

CAUTION

The centrifuge can be damaged if connected to the wrong voltage. Check the voltage before plugging the centrifuge into any power source. DuPont is not responsible for improper installation.

The centrifuge is equipped with a 3-wire power cord with a 3-prong cap, including ground connection, to fit Hubbell receptacle No. 9330 or equivalent.

To connect the centrifuge to a voltage other than what is specified on the nameplate, the centrifuge will have to be rewired and the power cord may have to be replaced. In this case,

contact DuPont to have a Service Representative do the rewiring.

The ON/OFF main power switch is a 30 A circuit breaker, therefore a separate line disconnect switch is not needed unless required by local codes.

2-3/2-4

Sorvall® Centrifuges Controls

Chapter 3

CONTROLS

This chapter provides:

• a detailed description of the RC-28S controls;

• a brief description of the built-in calculator; and

• a brief description of the diagnostic routines.

For step-by-step operating instructions see Chapter 4, and for further information on the calculator and the diagnostics, see Chapters 5 and 6.

NOTE

A convenient Operator's Guide containing simplified instructions is located under the centrifuge control console.

Control Panel

The RC-28S is easy to use. All commands are entered from the convenient keypad on the control panel and the set and run parameters are visible in the displays. All selected parameters can be locked to prevent changes to run settings. Figure 3-1 on the next page shows the RC-28S control panel and gives a brief description of the keys and displays. A complete description follows the illustration.

Set Display

The SET display is the lower display that changes by pressing the PROGRAM, CALCULATE or DIAGNOSTICS key to show various screens of parameters or operations. A cursor in the SET display indicates the field where the next input character will appear. Lights below the display indicate the position of the cursor.

Run Display

The RUN display includes the three upper displays that show actual speed, temperature, and time or Jco2dt during a run.

3-1

SCREEN INDICATOR

Indicates which screen has been selected and appears in SET display.

CURSOR INDICATOR

Indicates cursor location in SET display.

SET DISPLAY

Displays parameters to be selected, calculator modes, and diagnostic conditions. Change display by pressing one of

the three control keys be-

low the display.

RUN DISPLAY

Speed

Displays current rotor speed (rpm) during a run.

Temperature

Displays current rotor temperature (°C) during a run.

Time

TIMED Run: displays re-

maining run time. HOLD Run: displays ac-

cumulating run time.

displays accu-

mulating integral value.

Ja/WflNDICATOR

Indicator light is ON when lco2dt run is selected.

START

Starts the centrifuge run.

PROGRAM

Primary control key. Selects one screen of basic and three screens of op-

tional run parameters.

Display changes each

time key is pressed and a

corresponding indicator light above the key comes on to indicate which screen is in the display.

CALCULATE

Selects calculator mode to

perform basic centrifugation calculations. Four screens of calculations available. Display changes each time key is pressed and a corresponding indicator light above the key comes on to indicate which screen is in display.

DIAGNOSTICS

Selects diagnostic mode to help isolate malfunctions,

provide status of

various systems, and to

perform maintenance procedures.

ENTER

Moves the cursor right or left through SET display. Also causes an input value to be accepted.

ON/OFF +/-

The +/- changes set temperature values from plus to minus or from minus to plus.

The On/Off selects brake mode and other options.

STOP

Stops the centrifuge run.

NUMERIC KEYPAD

Used to input numbers to select parameters.

HOLD

Selects a continuous run.

CLEAR

Cancels an input or a previously entered value.

Sorvall® Centrifuges

Controls

Description of Display & Key Functions

j v j V j

Cancels the entry again,

Starts

Stops

the

The numeric keypad

select parameter values.

The ENTER keys cause accepted, plus they cause the right

On/Off word

On or Off

the SET the temperature value from positive negative

input

is set to

previously entered value

centrifuge

or a

previously entered value. When

zero

(0) and a new

run.

or to the

used

left through

select options. When pressed,

will appear

display.

positive.

value

restored.

input numbers from

input value

the

cursor

the SET

in the

The +/- is

can be

input. When pressed

appropriate field

used only

the key is

0 to 9 to

move

display.

negative

pressed,

to be

the

change

Selects a continuous the

SET

display is pressed, show accumulating

Selects four screens screens

come

the

The four PROGRAM parameters

are

labeled

on to

SET

indicate which screen

display will change

and

the

1, 2, 3, and 4 and

operations. A description

Rotor Speed Hr:Min Tenp/Max Brake

RCF Slow-Start Slow-Stop Zonal/Speed

run. The

and all

timer will start

run

zeros will

time.

Program

standard

you

key

to the

screens

next screen.

word Hold will appear

be in the RUN

and the

Key

and

optional centrifugation conditions.

have corresponding lights above

have accessed. Each time

are

illustrated below showing

of the

Time field

Functions & Display Screens

fields

each screen follows.

in the

display.

of the RUN

the key is

PROGRAM DISPLAY SCREENS

w2dt Tine-out Total Lock

HnMin field

soon

START

display will

PROGRAM

The

the key

the

available

that

pressed,

PROGRflM> Recal1# Save#

3-3

Controls Sorvall® Centrifuges

PROGRAM

Program Key Functions & Display Screens

Rotor Speed HrsMin Temp^Max Brake

Screen

F-28^13 23000

0:90

+4+9

When the "1" indicator light above the PROGRAM key will include temperature,

changed during a run.

Following is a description of each field in this screen.

the and

basic

brake.

run

All

parameters: rotor identification, speed, time,

parameters, except rotor identification,

on, the SET display screen

can be

Rotor Speed HrsMin TeMp/Max Brake

The SPIN-RIGHT® Rotor Management System automatically recognizes the installed Sorvall® rotor and RCF calculation. When the chamber door in the display. Whenever the chamber door the display unless the Lock display.

The rotor identification cannot be changed after START has been pressed.

Iliiiiiil Speed

Centrifugation speed increments of the installed rotor erroneous entry.

provide temperature compensation, top speed limit, speed control,

closed, the rotor name will appear

open, the word NONE will appear

on,

10 rpm.

which case the rotor name will stay

selected

If a

entered, all digits

this case, clear the entry and make a valid entry.

entering a value from 100

speed value greater than the maximum allowable speed

the Speed field will flash indicating an

28 000 rpm

the

NOTE

If an RCF value the chosen RCF value will automatically be calculated and displayed in this field.

The speed setting can Speed field and entering a new value.

selected

control the run, the speed required

changed after a run has been started by returning

achieve

Rotor Speed HrsMin TerwMax Brake

Centrifugation time can

Values are displayed exactly

displayed 0:90 which represents one hour and 30 minutes run time. The same run time

one hour and

displayed as 1:30.

3-4

be set

minutes can

from one minute entered;

for

example,

selected

to 99

hours and

you input 90,

entering 130; this will

minutes.

will

the

be be

Sorvall® Centrifuges Controls

PROGRAM

As soon

timer will begin when START from HOLD will appear

(0) timer will begin after Time entering a new value.

as an

set

value

and

will show accumulating

can be

entry to

changed after

zero in the SET

made,

show remaining

the

rotor

the run has

Program

the

pressed

display

run

Key

entered value will appear

and the RUN

time. When operating

has

been loaded

Functions & Display Screens

and the RUN

run

time.

started

display value will count down

display value will begin

and the

returning

HOLD

NOTE

the

integrator accumulated. During integral value

HnMin field. started.

During a timed

value will

displayed

is On, the run

a run, the RUN

and the SET

The

run

integrator cannot

with

in the

will

end

when

display will show accumulating

turned

preselected integral value,

G&dt

Total field

the

selected integral value

display will show

Off (or On)

Screen

the

Rotor 5r*eed HrsMin Tenp/Max Ilillfil

. i

in the

to the

after

RUN display.

selected,

Zonal mode,

chamber door closed.

Hr:Min field

the

accumulating

run

time

a run has

accumulating integral

the

has

in the

been

The word

zero

the

and

There

centrifugation temperature; protection limit.

Centrifugation temperature Acceptable value Temp value

to 0°C).

Max temperature

desired value, however,

operation, value until

are two

is not

the

temperature shown

value (shown

indicated temperature

Temp value

the Max

rotor temperature settings.

Max

the

temperature values

entered,

is -6°C or

or at

rotor

the

lower

in the SET

0°C.

can be

temperature setting should

has

and

changed

it cooled

the

other.

can be set

value will default

which case

Max,

from -20°C

are

from

NOTE

in RUN

display),

will decrease

must

to the

display

Max

moving

be at

least

selected temperature.

The

to 5°C

the Max

is 5°C or

temperature will remain

2°

first

one,

selects

0°C to

rotor cools until

the

not be

the

sample overtemperature

+40°C

+45°C. above temperature will always default

cursor

above

the

more above entered Temp

to Max and

set

Temp value.

changed from

Brak

Temp, selects desired

increments

If a Max

Temp value (unless

it is 5°

of 1°C.

temperature

3°C

above

entering

the

set

For

default

the

best

Press On/Off Brake field

turn

the

indicate choice.

brake

on or off. The

word

On or Off

will appear

in the

3-5

Controls Sorvall® Centrifuges

PROGRAM

Program Key Functions & Display Screens

When the brake is Off, the rotor will coast from set speed to zero rpm at the end of the run. If a slow stop has been selected, the rotor will coast to the transition speed, then continue to decelerate at the controlled rate. When the Brake is On, the rotor will brake from set speed to zero rpm at the end of a run; if a slow stop has also been selected, the rotor will brake to transition speed, then continue to decelerate at the controlled rate.

o *> "CF

screen

To access, press PROGRAM until the "2" indicator light above the key is lit. The

SET display will include the following fields: RCF, Slow Start, Slow Stop and Zonal.

RCF

100095 On fluto On fluio Off 1500

Slow-Start Slow-Stop Zonal/Speed

The RCF field is used to: (1) display calculated RCF value for the identified rotor and the set run speed prior to a run and to display actual RCF value during a run; or

(2) to set the centrifuge to operate at a given g force. When an RCF value is

selected to control the run, the speed required to achieve it will automatically be

calculated and displayed in the Speed field of Screen 1.

During a run, the RCF (at rmax of the identified rotor) at actual speed is always

displayed in this field whether the run is programmed for speed or g force. At the end of a run, the value zero will remain in this field until the chamber door is opened. When the door is opened, the display will change back to show the RCF

value for the set speed.

RCF Slow-Start

To select a slow start, move the cursor to Slow-Start and press will show "On Auto". Auto is the optimal rate automatically calculated to have the

installed rotor accelerate to transition speed in approximately 2 minutes. When the run is started, a message will appear in the SET display to indicate that a slow start is in progress. When the rotor reaches transition speed, the message in the

display will go out and the rotor will accelerate to set speed at the maximum rate. The length of time for acceleration from 0 rpm to transition speed can be changed by positioning the cursor under "Auto" and entering the number of minutes (from 1

to 30) that you want for acceleration. Once Slow-Start has been turned On, it will

remain On until it is turned Off. When Slow-Start is Off, the rotor will accelerate

from 0 rpm to set speed at the maximum rate.

On/Off;

the display

3-6

NOTE

The preset transition speed (approximately 300 rpm for S-20 rotor and approximately 800 rpm for all other rotors) can be changed. See Chapter 6.

Sorvall® Centrifuges Controls

PROGRAM

Program Key Functions & Display Screens

RCF Slow-Start Slow-Stop Zonal/Speed

To select a slow stop, move the cursor to Slow-Stop and press the On/Off key; the

display will show "On Auto". When On, the rotor will decelerate from set speed to transition speed at the rate controlled by the brake setting. When the rotor reaches transition speed, a message will appear in the SET display to indicate that a slow stop has been initiated and the rotor will decelerate at a rate that will take it approximately 3 minutes to reach 0 rpm. This rate of deceleration from transition speed to 0 rpm is automatically calculated for the rotor in use. The rate of

deceleration can be changed by positioning the cursor under "Auto" and entering the number of minutes (from 1 to 30) desired for deceleration. Once Slow-Stop has been turned On, it will remain On until turned Off. When Slow-Stop is Off, the rotor will decelerate at the rate controlled by the brake setting from set speed to 0 rpm.

NOTE

The preset transition speed (approximately 300 rpm for S-20 rotor and approximately 800 rpm for all other rotors) can be changed. See Chapter 6.

RCF Slow-Start Slow-Stop Zonal/Speed

Used to select the Zonal mode of operation when dynamically loading a sample and/or gradient into the Sorvall® TZ-28 Rotor. A Key# is required to activate the Zonal mode.

When the Key# is entered and accepted, the word On appears in the display and

the cursor moves to Speed. Enter the Zonal loading speed from 1300 to 2500 rpm

(in increments of 10 rpm). When the chamber door is closed and START is

pressed, the rotor will accelerate to the Zonal speed, at which time the chamber door can be opened and the sample/gradient loaded. After the sample and gradient are loaded, close the chamber door and the rotor will accelerate to the run speed selected in Screen 1 (the maximum Zonal run speed is 20 000 rpm).

At the end of the run, Zonal will turn Off automatically; therefore, each Zonal run

requires this option be turned On and the Key# entered. Zonal cannot be stored in program memory; the zonal speed can be saved, but the

option must be turned On before the run.

w2dt Tine-out Total Lock

1.97e07

This screen includes w2dt and Lock. To access, press PROGRAM until the "3" indicator light above the key is lit.

5.2QeQ7

3-7

Controls Sorvall® Centrifuges

PROGRAM

Program Key Functions & Display Screens

w2dt Tine-out Total Lock

The Ja)2dt option than a time value. Press On/Off previous

deceleration,

(ENTER

Time-out value, first enter a three digit (X.XX) coefficient value will automatically

the

During and

accumulating value. During a timed

value The integrator cannot

run

O) or

run.

integrator

the SET

and the

used

will automatically

and

Total

(START)

display will show selected Time-out integral value

Time-out value

program

is the

placed), then

run, the RUN

turned

value

the

at the

On or Off

a run to end at a

turn

the |

displayed: Time-out

at 0 rpm,

Time-out value

a two

beginning

digit exponent value. Press (START)

display will show accumulating integral value

run, the SET

after

u2dt 1 on. The

which includes deceleration. Press

and

deceleration.

a run has

desired integral value rather

J(o2dt values from

is the

begin

display shows

the run. To

been started.

value

w2dt Tine-out Total Lock

This feature locks

changed until unlocked. proper Key#. Once in

the

Lock field

enter

the

Key#.

all set run

The

run

conditions have been locked

and in all

conditions (including options)

Lock

calculator screens.

activated

pressing On/Off

in, the

unlock, press On/Off again

cursor will only appear

at the

(the

decimal point

and the

the

accumulating

they cannot

and

start

change

begin

Total

entering

the

and

The lock cannot

screen

Run conditions control centrifuge operation. however, a program

be stored

Chapter

To access, press PROGRAM until

and a

6).

stored

program memory.

PROQRflM> Recall* Sauett

are

stored

can be

different Key#

program memory

Key# must

recalled without

can be

the "4"

PROGRFlM> Recall** Save*

Used

recall the assigned program number (from will automatically appear run.

established program. Position

Screen

1 of the SET

and

entered

the

Key#. A total

assigned

indicator light above

1 to 20). The

the display. Press START

recalled from memory

save

each program,

the key is lit.

cursor recorded program parameters

a new

of 20

this field

programs

desired

program;

and

begin

can

(see

enter

the

3-8

Sorvall® Centrifuges

Controls

PROGRAM

Program

Key

PROGRRM> Recall* Sauett

Used

save

all set this field Key#. When

and

enter a number from

the assigned program number appears that

was

the program.

which case,

input becomes

the program numbers have been used, a new program.

old program

this case,

automatically deleted from memory.

The CALCULATE RCF,

speed,

below.

See

run

Chapter 5 for

run

conditions except Zonal

1 to

Key#

entered

and

in the

the

assigned program number that will

the

incorrect Key#

program number must

the

number assigned

Calculate

key

used

perform basic centrifugation calculations, including

time, Ju)2dt

and K Factor.

instructions

accepted,

entered,

Key

how

Functions & Display Screens

and

20. The the

Save#

and

Lock. Position

display will prompt

display shows "Saved"

Recall# fields.

the

you

The

the

display will show

reentered, then

program

can

stored

for

the

stored over

the

new

"Not

correct Key#.

program

Functions & Display Screens

The

four calculator screens

use the

calculator.

used

Saved";

are

cursor

enter

and

the

number

recall

all

existing

and the

CALCULATE

pictured

1 2 3 4

CflLO CRLO CflLO CRLO

Rotor

r(om)

Speed

Speed Speed K-Faciorl

Rrcax

Tinel

Diagnostics Key Functions & Display Screens

DIAGNOSTICS

used

monitor system operation, view system accumulators,

perform user diagnostic routines, install

calibrate replacement subassemblies.

screens

and

instructions

1 2 3

Fault

User-1 Maint-2

the use

Door- Sysien

User-2 Calib

See

this

new

key.

RCF

Tine w2dt

RCF

Rnin K-Factor2

rotors

and

Chapter

Maint-1 Serv-1

K-Factor

Tine2

software, troubleshoot,

6 for

examples

Instal

Serv-2

DIAGNOSTICS

and

display

3-9/3-10

Son/all® Centrifuges Operation

Chapter 4

OPERATION

This chapter provides:

• step-by-step operating instructions for the normal and zonal modes of operation; RCF and stop;

and HOLD runs;

• a description of the control panel lock and the Key# code;

• a precool/preheat procedure to equilibrate rotor and chamber temperature;

and

• a Rotor Information Chart.

Jco2dt

operation; programmed runs; slow start and slow-

Understanding the Centrifuge Controls

It is recommended that you read the description of the controls in Chapter 3

before using the centrifuge for the first time. You probably won't use all of the

controls right away, but reading Chapter 3 will give you information that will be helpful in understanding the displays and how the control keys function, and it will help you become familiar with all features of the centrifuge. As you use the centrifuge, continue to refer to Chapter 3 whenever additional information is

needed.

Turning the Centrifuge On

The power ON/OFF switch is placed in the upper right hand corner of the front cabinet panel. The microprocessor automatically runs a diagnostic test everytime the centrifuge is turned on. If an error is found, a display message will appear in the SET display on the control panel. If no error is found, the run parameters will appear in the display indicating that the centrifuge is ready for operation.

Normal Run

A normal run is one that is controlled by time with selected run temperature and

run speed parameters and the brake set on or off. These parameters are selected in the first screen of the (PROGRAM) key.

Prior to performing the run, prepare and install the rotor according to the

instructions in the rotor manual. To ensure that the sample, rotor, and chamber temperatures are at equilibrium at the start of the run, follow the precool/preheat procedure on page 4-12.

4-1

Operation Sorvall® Centrifuges

WARNING

When loading the rotor, be sure not to exceed the maximum compartment mass of the rotor (see Table 4-1, Rotor Information). If maximum compartment mass is exceeded, the maximum operating speed must be lowered

(see Reducing Speed for Rotor Compartment Loads In Excess of Design Mass,

page 4-12). Failure to do so can cause rotor failure which could result in

personal injury and/or centrifuge damage.

To perform the run:

Turn the centrifuge power ON.

NOTE

For best run conditions and prolonged centrifuge life, the rotor chamber should be dry at the start of a run. Before installing the rotor, wipe chamber

dry with a clean cloth or papertowel.

Open chamber door all the way and install rotor. Close chamber door.

WARNING

Use Sorvall® rotors only. Use of another manufacturer's rotor can cause rotor failure which could result in personal injury and/or centrifuge damage.

The

"1"

light above the (PROGRAM) key should be on. If not, press (PROGRAM) until it is.

The name of the installed rotor will appear in the display and the cursor will be positioned in the | Speed I field.

NOTE

If using an to the 1 Rotor 1 field and input 18 for rotor will be recognized as an S-20/36. Press (ENTER O).

Input desired run parameters: | speed|, lHr:tiinl, and jTer>p/riax|. Press (ENTER E>) after

each input. Max temperature will automatically default to 5°C above set run temperature; if

desired, enter a different value. For a continuous run, press (HOLD).

Press (onoft) to make 1 Brake 1 selection. The word |0n| or [off | will appear in the

display to indicate selection made.

S-20/17

S-20/20

rotor ONLY: Press (ENTER <J) to move the cursor

S-20/17

or 17 for S-20/20; otherwise the

After you have selected the above parameters, the SET display should look similar

to the example below:

Rotor Speed HrsMin Tenp/Max Brake

F-28/13 28090

4-2

0:90

+4+9

Sorvall® Centrifuges Operation

6. Select [siou-start] and/or [siou-SioH if desired (see below).

Press (START).

Any of the run parameters, except rotor identification, can be changed while a run

is in process.

The rotor will accelerate to the set run speed and remain there for the selected

length of time then decelerate to a stop. To end a run in HOLD or before the selected run time has elapsed, press (STOP).

NOTE

Integral value can be checked anytime during a timed run by pressing (PROGRAM) until | u2dt | is in the display ("3" indicator light on). The accumulating fa)2dt value will be in the | Total 1 field.

To do another run with the same parameters: prepare rotor and install it in the

centrifuge, then close chamber door and press (START) . All entered parameters

remain until a change is made.

Slow Start/Slow Stop

Slow Start and Slow Stop are used to prevent gradient disturbance during acceleration and deceleration. When they are on, the rotor will accelerate or

decelerate to or from transition speed at a controlled rate that is slower than normal (transition speed is preset at approximately 800 rpm for all rotors except the S-20, which is preset at approximately 300 rpm; preset transition speed can be changed, if desired - see Chapter 6). The length of time for the acceleration/ deceleration phase will automatically default to the optimal time for identified rotor; or, if desired, time (from 1 to 30 minutes) can be entered manually.

NOTE

If Slow Start or Slow Stop are on, they will remain on for future runs until

turned off.

Follow Steps 1 through 5 for a Normal run (page 4-2).

Press (PROGRAM). The "2" indicator light above the key will light.

To select Slow Start:

Press (ENTER O) to move cursor to |siou>-stari|.

Press (on off).

To accept the "Auto" time for acceleration and begin the run, press (START).

RCF blow-Start blow-Stop Zonal/Speed

100995

To change the length of time for acceleration from 0 to transition speed, press

(ENTER c>) to move cursor to | Ruto

30).

If a slow stop is desired (see page 4-5); if not, press (START) to begin the run.

fluto

Off Off 1509

and input desired number of minutes (from 1 to

4-3

Operation

Use Sorvall® rotors only. Use of another manufacturer's rotor can cause rotor failure which could result in personal injury and/or centrifuge damage.

Sorvall® Centrifuges

Table 4-1. Rotor Information

WARNING

ROTOR

SV-288

SV-80

GS-3

SA-600

SS-34

SE-12

HB-4

HS-4

SM-24

GSA

TZ-28 SH-MT SH-80

CODE

2 3

11 12 13

MAX

SPEED

(rpm)

20 000

19.000 9000

16 500

20 000

21000

13 000

7000

20 000

13 000

20 000

13 730

20 000

CRITICAL

SPEED

(rpm)

1000

1600 1650

1200 1050

750

1200

1350 1700 1200

1000

1300

900

MAX

RCF

40 301

40 96$

13 689

39 411

47 807

45 959

27 579

9425

49 460

27 504

42 535

19140

45 394

FACTOR

4201

1678

4487

2019

210

792

750

513

457

605 656 399

COMPART-

MENT MASS

250.0 g

1035,0 0

680.0 g

1620.0 g

MAX

50.0 g

6.4 g

780.0 g

115,0$

115.0 g

30,0 $

27.0 g

36.4 g

78.0 g

MAX (cm)

9.02

10.16

15.13

12.96

10.70

9.33

14.61

17.22

11.07

14J7

9.52

9.Q9

10.16

MIN

(cm)

6.47

8.84

3.93 5,52

3.26

3.81

4.75

7.21

5.37

3.77

3.65

5.57

5.40

F-28/13

F-28/36

3-20/36

S-20/20

S-20/17

SS-34/KSB-R

TZ-28/GK

With maximum allowable volume at maximum speed.

Outer row

Maximum speed of rotor is 20 000 rpm; actual maximum speed depends on tubes. Values given are typical for 1.5 ml microtubes.

28 000

20 000

19 000

1300

400

1350

1000

100 09$

100 095

71934 58 083

7416S

47 807

38 38$

143

198

466

299

573

750

671

23,0 8

66.0 g

. 156.6 g

115.1 g

130.1 $

115.0g

1620.0g

11x43

11.43

16.10

13.00

16*60

10.70

9.52

4-4

7,34

6.18

7.70

8.10

6.70

3.26

3,65

Sorvall® Centrifuges Operation

As soon as (START) is pressed, the |*** sioui start in Progress ***[ message will appear in

the SET display and will remain there until the rotor has accelerated to approximately 20 rpm.

To select Slow Stop:

Press (ENTER E>) to move cursor to [Sloui-stop].

Press (on off).

To accept the "Auto" rate of deceleration and begin the run, press (START).

RCF Slow-Start Slow-Stop Zonal/Speed

190095 On Ruto On Ruto Off 1500

To change the length of time for deceleration from transition speed to 0 rpm,

press (ENTER O) to move cursor to I nuto | and input desired number of minutes (from

1 to 30). To begin run, press (START).

During deceleration, the | *** siou stop in Progress *** [ message will appear in the SET display as soon as the rotor reaches transition speed; the message will remain

in the display until the rotor stops spinning.

RCF Run

The centrifuge can be set to operate at a given g force rather than speed. When you know the RCF (g force) you want applied to the particles during centrifugation, you can use this feature to select the value. The run speed required for the set RCF value will automatically be calculated.

To perform an RCF Run:

Load and balance the rotor according to the instructions in the rotor manual. To

ensure that the rotor and chamber temperatures are at equilibrium, follow the

precool/preheat procedure on page 4-12.

WARNING

Use Sorvall® rotors only. Use of another manufacturer's rotor can cause rotor failure which could result in personal injury and/or centrifuge damage.

Do not exceed maximum compartment mass of the rotor (see Table 4-1, Rotor Information). If maximum compartment mass is exceeded, the maximum operating speed must be lowered (see Reducing Speed for Rotor Compartment Loads In Excess of Design Mass, page 4-12). Failure to do so can cause rotor failure which could result in personal injury and/or centrifuge

damage.

Turn the centrifuge power ON.

4-5

Operation Sorvall® Centrifuges

NOTE

For best run conditions, the rotor chamber should be dry at the start of a run.

Before installing a rotor, wipe the chamber dry with a clean cloth or

papertowel.

Open chamber door all the way and install rotor. Close chamber door.

The

"1"

light above the (PROGRAM) key should be on. If not, press (PROGRAM) until it is.

NOTE

If using an S-20/17 or S-20/20 rotor ONLY: Press (ENTER O) to move the cursor to the 1 Rotor 1 field and input 18 for an S-20/17 or 17 for an S-20/20; otherwise

the rotor will be recognized as an S-20/36. Press (ENTER O).

5. Input desired lHr:Minl and | Tenp/Max | values. Press (ENTER O) after each input. (Speed is not entered because run speed will automatically be calculated and set when

the RCF value is entered.) Max temperature will automatically default to 5°C above set run temperature; if

desired, enter a different value. For a continuous run, press (HOLD).

6. Press (on off) to make 1 Brake I selection. The word |0n | or joff | will appear in the display to indicate selection made.

7. Press (PROGRAM) again. I

RCF I will appear in the display with the cursor positioned in the I RCF 1 field and the

"2"

light above the (PROGRAM) key will be on.

8. Input desired |RCF| value (for rmax of the installed rotor). Press (ENTER O).

The display should look similar to the example below.

RCF Slow-Start Slow-Stop Zonal/Speed

93180 Off Off Off 1500

9. Press (START).

The rotor will accelerate to the speed required to achieve the set RCF value and remain there for the selected length of time or until (STOP) is pressed, then

decelerate to a stop.

Key# Code

The Key# is a security number that must be entered in order to access Zonal, Program Save, the control panel Lock, and some Diagnostic routines. Whenever you attempt to use these features, the display will prompt you to enter the Key#.

4-6

Sorvall® Centrifuges Operation

A different number can be assigned to each of the features that require a Key#,

including a different number for each of the 20 programs saved. There is also a System Access Key# that allows access to any of the fields that require a Key#, except for some Diagnostic routines. The centrifuge is shipped with the System

Access Key# 1234. This is the only number assigned when you receive the

centrifuge. For the procedures to change the System Access number or to assign

Key#s to the various features, see Chapter 6.

Zonal Run

Zonal operation allows you to operate the centrifuge with the door open at speeds from 1300 rpm to 2500 rpm. This is used with a Sorvall® TZ-28 Zonal Rotor to dynamically load gradient and sample. A Key# is required to access Zonal mode.

Assemble the rotor according to the procedure in the rotor instruction manual. To

ensure that rotor and chamber temperatures are at equilibrium at the start of the

run, follow the precool/preheat procedure on page 4-12.

To perform the run:

Turn the centrifuge power ON.

Open chamber door all the way and install rotor. Close chamber door.

The

"1"

light above the (PROGRAM) key should be on. If not, press (PROGRAM) until it is.

Input desired run parameters: I Speed I, |Hr:Minl and | Tenp/Max]. Press (ENTER O) after

each input.

NOTE

Maximum speed in the Zonal Mode is 20 000 rpm.

Max temperature will automatically default to 5°C above set run temperature; if desired, enter a different value. For a continuous run, press (HOLD).

Press (on off) to make | Brake I selection. The word

display to indicate selection made.

6. Press (PROGRAM) until the "2" light above the key is on and [ Zonal /Speed | appears in the display.

[ On

| or |off [ will appear in the

NOTE

It is best to use a slow start and slow stop when doing gradient separations in

the TZ-28 Rotor (see Slow Start/Slow Stop, page 4-3).

Select Isiou-start] and/or lsiou-stop] if desired.

8. Press (ENTER t>) to move the cursor to [ Zonal /Speed [. Press (on off). The display will

prompt you to enter | Ke^tt | (page 4-6).

9. Input | Ke^tt I and press (ENTER O). When the Key# has been accepted, the word

will appear in the display indicating that the centrifuge will operate in the Zonal mode.

I On I

4-7

Operation Sorvall® Centrifuges

10.

Input the desired loading speed (from 1300 rpm to 2500 rpm in increments

of 10). Press (ENTER D>).

At this point, the display should look similar to the following:

RCF Slow-Start Sloui-Stop Zonal/Speed

42535

When rotor reaches selected loading speed, the I*** Rotor at Zonal Speed> Load ***| message will appear in the display indicating that the door can be opened.

When operating the centrifuge with the chamber door open and the rotor

spinning, remove any articles that could fall into the chamber (e.g., jewelry,

necktie, objects in shirt pockets). Tie back long hair and roll up shirt sleeves.

12.

Open chamber door and load the sample/gradient according to the instructions

in the rotor manual. When loading is complete, disconnect and remove any

equipment that was used to load it.

Off Off On 1500

WARNING

When loading the sample and gradient, be sure not to exceed maximum

compartment mass specified for the rotor (see Table 4-1, Rotor Information). If maximum compartment mass is exceeded, operating speed must be lowered (see Reducing Speed for Rotor Compartment Loads In Excess of Design Mass, page 4-12). Failure to do so can cause rotor failure which could

result in personal injury and/or centrifuge damage.

13.

Close the chamber door. The timer will begin and the rotor will accelerate to

the set run speed and remain there for the selected run time. At the end of the run, the rotor will decelerate to 0 rpm and the Zonal function will turn off

automatically.

Hold Run

A Hold run is a run that will continue until (STOP) is pressed. All parameters except

time are entered as described for the type of run you will perform.

To select Hold:

Press (HOLD). Hold will appear in the iHrtliinl field of the SET display. While the

run is in process, the RUN display will show the accumulating time.

To end a HOLD run:

Press (STOP). The rotor will begin to decelerate and the run will end.

4-8

Sorvall® Centrifuges Operation

To change a HOLD run to a timed run:

Press (HOLD). The key is a toggle switch so pressing it while in HOLD will return the centrifuge to a timed condition. The last entered time value will appear in

the iHr:Hinl field of the SET display and the RUN display will show the remaining time.

NOTE

When the centrifuge returns to a timed condition, the computer will account for the length of time the centrifuge was in HOLD. Therefore, if the last entered time value had already elapsed when the HOLD key was pressed again,

the RUN display will show zeros (0.00) and the rotor will begin to decelerate.

To enter a new time value and cancel the accumulated HOLD time:

Enter the new time value. The |Hr:Min| field of the SET display will show the new set time, and the RUN display will change to countdown from the newly entered value. The length of time the centrifuge was in HOLD has no affect on

the new set time.

fw2dt Run

An fa)2dt run allows you to set the centrifuge to end a run when a desired integral value is reached.

To perform an fo>2dt run:

Load and balance rotor according to instructions in the rotor manual. To

ensure that the rotor and chamber temperatures are at equilibrium, follow the

precool/preheat procedure on page 4-12.

WARNING

Use Sorvall® rotors only. Use of another manufacturer's rotor can cause

rotor failure which could result in personal injury and/or centrifuge damage.

Do not exceed maximum compartment mass of the rotor (see Table 4-1, Rotor Information). If maximum compartment mass is exceeded, the maximum operating speed must be lowered (see Reducing Speed for Rotor Compartment Loads In Excess of Design Mass, page 4-12. Failure to do so can cause rotor failure which could result in personal injury and/or centrifuge damage.

Turn the centrifuge power ON.

NOTE

For best run conditions, the rotor chamber should be dry at the start of a run. Before installing a rotor, wipe the chamber dry with a clean cloth or

papertowel.

4-9

Operation

Open chamber door all the way and install rotor. Close chamber door.

The

"1"

light above the (PROGRAM) key should be on. If it is not, press (PROGRAM) until

it is.

NOTE

Sorvall® Centrifuges

If usingan_S-20/17 or S-20/20 rotor ONLY: Press to the [Rotor| field and input 18 for an S-20/17 or 17 for an S-20/20; otherwise

the rotor will be recognized as an S-20/36. Press (ENTERED).

5. Input desired I Speed! and press (ENTER >) twice to move the cursor to | Tenp/Max I. (Time is not entered since the run will end when the integral value has

accumulated.)

6. Input desired 1 Tenp/Max 1 values. Press (ENTER PQ after each input.

7. Press (on off) to make [Brake! selection. The word [On] or | Off | will appear in the

display to indicate the selection made.

8. Press (PROGRAM) until the "3" light above the key is lit and |

display. The word [on] or [off] will appear in the | u2dt | field with the integral values

from the previous run displayed next to it. See example below.

CENTER<Q

to move the cursor

u>2dt I

appears in the

NOTE

Tine-out | is the Total | is the fafdt value from the previous run at 0 rpm, which includes the

deceleration time.

J(o2dt

value from the previous run at the start of deceleration.

u2dt Tine-out Total Lock

1.94e07

5.29e07

Off

9. If Off, press (on off) to turn On.

10.

To use the |Tine-out) value, press (START). TO change the [ Tine-out 1 value, press

(ENTER

a two digit exponent value. Press (START).

The rotor will accelerate to the set speed and remain there until the integral value

has accumulated.

To end a run before the selected integral value has accumulated, press (STOP).

t>) and enter a new value: first a three digit (X.XX) coefficient value, then

Programmed Run

The RC-28S memory will store up to 20 programs. Each program saves a set of run

parameters that can be recalled by entering the assigned program number (from 1 to 20) and pressing (START).

4-10

Sorvall® Centrifuges Operation

A Key# must be entered in order to store a new program or to make a change to an existing program; however, a stored program can be recalled without the Key#. A different Key# can be assigned to each program, if desired; see Chapter 6 for procedure.

Program Save

Enter all desired run parameters.

NOTE

Program Save will not store if the control panel Lock is on; also, Program

Save will not turn Zonal On.

Press (PROGRAM) until the "4" indicator light above the key is lit.

Press (ENTERO) to move the cursor to fsavettl and input a number from 1 to 20.

Press CENTER

Input 1 Ke'd# | (page 4-6). Press (ENTER O) to store program. Press (START) to begin the

run.

PROGRflN > Reca11# Save#

Record program number and run parameters in the Program Log Record

(located in back of the Operator's Guide under the centrifuge control panel).

NOTE

Program Recall will not turn Zonal On, but it will set loading speed and all

other parameters.

Program Recall

To recall a program:

Press (PROGRAM) until the "4" indicator light above the key is lit.

Input assigned program number (from 1 to 20). Press (START).

All prerecorded run parameters will automatically be established to control centri-

fuge operation. The displays will change to show the set run parameters for the selected program.

Saved

Control Panel Lock

The control panel Lock will lock all set run conditions (including options) so they

cannot be changed until unlocked.

To lock or unlock set run conditions:

Press (PROGRAM) until the

"3"

indicator light above the key is lit.

4-11

Operation Sorvall® Centrifuges

Press (ENTER oj to move the cursor to I Lock].

Press (onoff) and input 1 Ke*#l (page 4-6). Press (ENTER O).

After run conditions have been locked in, the cursor will only appear in the [Lock field and in all calculator screens.

Precool/Preheat

The following precool/preheat procedure is recommended to ensure that the rotor and the chamber temperatures are at equilibrium at the start of a run.

Open chamber door and install rotor. Close chamber door.

Press (PROGRAM) until the

Input I speed I of 2000 rpm. Press (ENTER O).

Press (HOLD), then press (ENTER O).

Input desired iTenpl. Press (ENTER O).

"1"

light above the key is lit.

6. Press (START) . The rotor/chamber should achieve temperature in approximately 30 minutes.

Reducing Speed for Rotor Compartment Loads

in Excess of Design Mass

There is a maximum allowable compartment mass established for each centrifuge rotor (see Table 4-1, Rotor Information). To prevent rotor failure, the total

contents of any compartment, including specimen, tubes, covers, and adapters (if used), proportionately.

Strict adherence to the maximum allowable compartment mass or reduced speed

(see below) is required to prevent rotor failure. Observe WARNING on the Safety

Information Page in front of this manual.

The rotor speed is reduced in proportion to the square root of the ratio for the

maximum allowable compartment mass to the actual compartment mass (including

specimen, tubes, covers, and adapters). If the compartment mass is more than

that specified for the rotor, the reduced speed can be determined by using the

formula given below.

must not exceed the figure given in Table 4-1 unless rotor speed is reduced

4-12

„_,_,„ Reduced

^ ... „ « _, * I Maximum Compartment Mass

Rotor Speed = Max.mum Rotor Speed x \ Actual Compartment Mass (grams)

Sorvall® Centrifuges

Calculator

Chapter 5

CALCULATOR

The RC-28S calculator allows you to perform basic centrifugation calculations to

help determine run conditions necessary to provide desired separation results. Four screens of calculator functions are available. When you enter the CALCULATE

mode, the values displayed in Screens 1, 2 and 3 are calculated from the data entered in the first three screens of the PROGRAM mode. To do calculations for a different rotor, enter the rotor code number (see Table 4-1, Rotor Information) in j

Rotor 1 field of CALCULATE Screen 1. The values displayed in Screen 4 (if any) are

from the previous calculation in that screen.

CALCULATOR DISPLAY SCREENS

2 3 4

CRLO CflLO CRLO CflLO

Rotor Ken) Speed

Speed Speed

Rroax

K-Factorl Tinel

RCF Tine

RCF

Rnin

K-Factor

K-Factor-2

w2dt

Tine2

liBIllillll CflLO Rotor Speed RCF Tine w2dt

Use to calculate SPEED, RCF (at rmax), TIME, and fo)2dt. To access, press

(CALCULATE) until the "1" indicator light above the key is on. Enter rotor code

number (see Rotor Information Chart) in the j Rotor 1 field.

To calculate SPEED from RCF:

Position cursor in

Press (ENTER O»). The calculated speed will be displayed.

To calculate RCF (at rmax) from SPEED:

Position cursor in 1 Speed 1 field and input speed.

Press (ENTER o). The calculated RCF value will be displayed.

|RCF 1 field and input RCF value.

To calculate estimated run TIME from

Position cursor in | Speed 1 field and input speed.

Press (ENTER t>) to move cursor to | u>2dt | and input Jafdt value.

Press (ENTER O). The calculated run time will be displayed.

To calculate estimated

Position cursor in | Speed [field and input speed.

Press (ENTER O) to move cursor to [Tinel and input time.

Press (ENTER t>). The estimated integral value will be displayed.

* It is not possible to exactly calculate Time from

approximate value to help you save time.

fa>2dt

from SPEED and TIME: *

j<o2dt

J<a2dt

or\w2dt from Time. These calculations are meant to give an

and SPEED: *

5-1

Calculator Sorvall® Centrifuges

Calculator Use

Screen 2 ICflLO r(cn) Speed RCF

Use to calculate RCF or SPEED at a rotor radius other than the maximum. To

access, press (CALCULATE) until the "2" indicator light is on. The values in the display are from Screen 1 of the (CALCULATE) key.

To calculate SPEED from RCF at a radius other than rmax:

Position cursor in iKcrOl field and input radius.

Press (ENTER O) to move cursor to |RCF 1 and input RCF.

Press (ENTER P). The calculated speed will be displayed.

To calculate RCF from SPEED at a radius other than rmax:

Position cursor in lr<cn)| field and input the radius.

Press (ENTER O) and input [SpeedI.

Press (ENTER P). The calculated RCF value will be displayed.

CRLO Speed Rmax Rnin K-Factor

Use to calculate K Factor. To access, press (CALCULATE) until the "3" indicator light above the key is on. The values in the display are from Screen 1 of the (CALCULATE) key.

To calculate K Factor at a particular speed using rmin and rmax diplayed:

Position cursor in 1 Speed | field and input desired speed.

Press (ENTER P). The calculated K Factor will be displayed.

To calculate K Factor using an rmin or rmax other than what is displayed:

Verify speed and change if desired.

Move cursor to | Rrcax | and/or [Rninl field and input radius value.

Press (ENTER P). K Factor will be calculated and displayed.

Screen 4 CfiLO K-Factorl Tinel K-Factor-2 Tine2

Use primarily for pelleting runs to convert centrifugation time from one rotor (Rotor-1) to another rotor (Rotor-2). To do so, you need to know the K Factor at desired run speed for both rotors.

To calculate:

Press (CALCULATE) until the "4" indicator light above the key is on.

Input K Factor of

Input run time from Rotor-1 in iTineil field. Press (ENTER P).

Input K Factor of Rotor-2 in 1K-Factor2l field. Press (ENTER P). The run

time calculated for Rotor-2 will be displayed in jTine2| field.

Rotor-1.

Press (ENTER P).

* K Factor (the rotor clearing factor) is a function of sample path length (distance between rmin and rmax) and rotor speed. The

lower the K Factor value, the faster a given centrifugation protocol can be completed. Protocols can be converted from one rotor to another if K Factors are known for both rotors.

5-2

Sorvall® Centrifuges Troubleshooting

& Diagnostics

Chapter 6

TROUBLESHOOTING

and DIAGNOSTICS

This chapter provides:

• a description of SET display messages;

• DIAGNOSTICS information;

• the procedure to assign or change Key#'s; and

• the procedure to change the Slow Start/Slow Stop transition speed.

Display Messages

The RC-28S has a number of display messages that can appear in the SET display

on the control panel. These messages notify you of an operating condition or a centrifuge fault that, in most cases, requires attention or demands action.

Following is a list of the SET display messages, along with the probable cause (in bold print) and the corrective action.

***

Check Installed Rotor

The rotor management system did not recognize the installed rotor. Possible causes are:

the rotor management system was not properly activated;

the installed rotor is not intended for use in the RC-28S;

a rotor code was manually input that is incorrect for the installed rotor; or

rotor/rotor lid not properly installed.

To reactivate rotor management system, open the chamber door all the way and

close it again slowly.

If the installed rotor is not meant for use in this centrifuge, remove it and install

one that is (see Table 4-1, Rotor Information).

Use Sorvall® rotors only. Use of another manufacturer's rotor can cause rotor failure which could result in personal injury and/or centrifuge damage.

*#*

WARNING

Install rotor to match selected rotor code; or, if the rotor you want is installed,

clear the message by opening and closing the chamber door. The rotor

management system will automatically recognize the installed rotor.

Properly install rotor/rotor lid; refer to rotor instruction manual.

6-1

Troubleshooting Sorvall® Centrifuges & Diagnostics

*## Close Chanber Door

The chamber door

the

chamber door

the door

Centrifuge malfunction.

Press (DIAGNOSTICS)

(page

all the way

***

Fault> Press DIRGNOSTICS ***

6-5) for

more information.

***

There

pressed.

Open

was no

the

chamber door

was not

first, then close

shut

Install Rotor

rotor installed when

***

completely closed when

all the way and

it.

off

alarm

press (START);

and

select DIAGNOSTIC mode.

***

the

chamber door

and

install a rotor. Close

the

(START)

it may be

was

door

and

key was

necessary

See

closed

press (START).

and

pressed.

open

Diagnostics

(START)

was

**# Open Chamber Door

Run

has

ended.

Open chamber door

***

Power Outage> Run Interrupted ***

A power outage occurred during restored.

No corrective action required. Press (PROGRAM)

**# Pouer Outage> Run Terminated **#

A power outage occurred during temperature significantly varied from

Restart

run.

and

remove rotor.

*##

the run. The run

the run. Run

set

parameters.

resumed when power

clear message.

terminated because speed and/or

was

6-2

Sorvall® Centrifuges Troubleshooting

& Diagnostics

*** Recalled Rotor Hot Installed ***

A program was recalled that requires a rotor different than the one installed in the

chamber.

Install the correct rotor for the program recalled, or verify that you used the correct program number.

*#*

Release Door Handle ***

Indicates that you tried to open the door before the door latch had released.

Release the door handle and wait until the message clears, then open the door. If the problem does not clear, the door latch solenoid may be defective.

*#*

Replace NOURflM Battery Soon *#*

Battery for the centrifuge memory is low and must be replaced. (Average battery life is from 5 to 10 years.)

Call for Service to replace battery.

#** Rotor at Zonal Speed> Load #*#

Zonal run is in process and rotor is at selected loading speed.

Open chamber door and load sample/gradient. Close chamber door.

*** Rotor Imbalance ***

Excessive rotor imbalance; run terminated.

Remove the rotor and balance it according to the instructions in the rotor manual, then install rotor and restart the run.

*** Rotor Overtenperature *#*

Run terminated because display temperature is higher than selected 1 Max temperature. Possible causes are: (1) selected 1 Max 1 temperature set below displayed temperature; (2) rotor not precooled; (3) selected 1 Max [ low for the rotor/speed combination; (4) high ambient temperature; or (5) condenser inlet blocked.

temperature too

Check possible causes: (1) if 1 Max 1 temperature was manually set lower than display temperature, run will terminate when (START) is pressed; wait until actual temperature cools to 1 Max 1 temperature setting and restart run or set a higher | temperature and press (START) to restart run; (2) if rotor was not precooled, precool

Max

6-3

Troubleshooting Sorvall® Centrifuges

& Diagnostics

it according to procedure on page 4-12; (3) acceptable rotor/speed/temperature

combinations vary depending on rotor used; in some cases, if temperature is set lower than +2°C, rotor temperature may not be attainable at set speed; to achieve temperature, set a lower speed; (4) check ambient temperature: it should be between 15°C and 25°C for optimal performance; will operate at temperatures up to 38°C but performance will be less than optimal; (5) check for 6 inch clearance on left side of centrifuge; clean condenser (to clean the condenser, see Chapter 7). If problem does not clear, there may be a refrigeration system malfunction. See DIAGNOSTICS.

*#*

Secure Rotor Properly

The rotor is not properly locked on the centrifuge drive.

Open the chamber door and tighten rotor locking knob(s); if the rotor has two knobs, tighten the larger one first. Close chamber door and press (START).

***

Self Test

Progress

*#*

***

A diagnostic test of all centrifuge systems. This is done once every 24 hours that

the centrifuge power is ON.

No corrective action required.

##* Slow Start in Progress ***

Indicates that a Slow-Start was selected and initiated.

Message will clear automatically when rotor has accelerated to transition speed; no

corrective action.

*## Slow Stop

Indicates that a Slow-Stop was selected and initiated.

Message appears when the rotor has decelerated to transition speed. It will clear as soon as the rotor has stopped spinning; no corrective action.

Tach Failure> Rotor Spinning

Progress

*#*

***

Tachometer has failed. Run is terminating, but the rotor is still spinning, even though RUN display may indicate 0 rpm. The chamber door cannot be opened until the rotor has come to a complete stop.

Wait until the

chamber door and remove the rotor. Call for Service.

6-4

I***

Open chamber Door ***[rnessage appears in the display, then open the

Sorvall® Centrifuges

Troubleshooting

& Diagnostics

*** Tine

Twenty

Change vacuum pump

** Turn DIRGNOSTICS

The DIAGNOSTIC mode

Service Uacuun Punp

***

less vacuum runs remain before vacuum pump

oil as

Off

was

soon

possible (see Chapter

Before Start

exited with

one of

not start.

Press (DIAGNOSTICS) twice. Press (on Press (PROGRAM)

***

Uacuun Punp Must Be Serviced ***

exit DIAGNOSTICS

off) to

and

turn

start

the

run.

Vacuum will not operate until vacuum pump oil if set speed

higher than

the top

air speed*

rotor.

Change vacuum pump

oil

(see Chapter

7).

oil

must

changed.

7).

the fields turned on; centrifuge will

field

off.

Input

changed. Centrifuge will not start

the installed Sorvall® Supraspeed®

and

press (ENTER

O).

* Top

air

speed

equal

maximum speed

the rotor in an RC-5C Centrifuge. See rotor instruction manual.

Diagnostics

General

The DIAGNOSTIC information provided understand tasks that require

the

basics

of the

the use of

DIAGNOSTIC mode

DIAGNOSTICS. Common tasks done using

DIAGNOSTIC mode include assigning Key#s, changing slow start/slow stop transition speed, identifying system faults, shooting. Other routines

are

available qualified service personnel only, therefore, instructions included

best The illustrations below

(Screen

this manual.

call

4 is

currently

Pont

For

for

assistance.

are

not

Fault

of 2

information regarding

representative

used.)

Door Systerc

this manual

and to

DIAGNOSTICS that

use of the

of the

intended

teach

the

you to

vacuum pump

are

designed

for

their

service routines,

three DIAGNOSTIC screens.

help

you

perform routine

the

oil and the

and

trouble-

for use by

use are not

it is

User-l

Off

Maint-2

Off

User-2

Off

Calib

Off

Maint-l

Off

Serv-1

Off

Install

Off

Serv-2

Off

6-5

Troubleshooting & Diagnostics

Sorvall® Centrifuges

Following

Screen

Screen 1 identifies faults

1***

Fault> Press DIRGNQSTICS *** [ will appear the cursor indicator lights beneath alarm will sound every minute until (DIAGNOSTICS)

When (DIAGNOSTICS) The fault information

System, Vacuum System); second, specific cause(s) Failure, Vacuum Release Failure). As soon fault description will appear faults have occurred (e.g., Fault two general faults occurred, the first being

Press (ENTER O). When you do, the fault information a specific cause for the fault along with a string of binary numbers. Each" 1" of binary numbers represents a separate cause for the fault. If there is more than one press (ENTER another cause

is an

explanation of how to enter and move around

Faults

and

pressed, descriptive fault information will appear

arranged

Fault

1 of 2

again and the cursor will move

for

the fault.

gives specific causes

the

display will move from left

two groups: first, general faults (e.g., Refrigeration

in the

1 of

Door System

display with

1, Fault 1 of 2, etc.). The example below shows that

the SET display, an alarm will beep, and

(DIAGNOSTICS)

the Door System.

the next" 1" and the display will show

the DIAGNOSTIC mode.

for

them. When a fault occurs,

pressed and the fault

for the

indication

the display will change

fault (e.g., Compressor

pressed, the first general

how many general

right.

cleared.

in the

display.

the string

The

show

"1",

Continue returned

Fault

1 of

change

Fault

of 2

press (ENTER

to the

show the next general fault;

|Fault| field.

3), when the cursor returns

Door

Not

until

there was more than one general fault (i.e., Fault

Locked

all

causes have been displayed

the Fault field,

for

example:

Fault Uacuun Systen

of 2

Continue screen again includes the first general fault (e.g., Fault

displayed; this information will

troubleshoot the problem.

After |»»* not clear when (STOP) to clear

faults were displayed.

press (ENTER

until

all

faults have been displayed

NOTE

you

move through

all

faults have been identified

Fault > Press DlRGNOSTics ***| message from the display.

the

message. Call

the

fault screens,

pressed, turn

for

sure

required

and

Service; when you call,

written down, press

the

centrifuge power OFF then back ON

and

the

display will automatically

and the

1 of

2, Door System).

make a note

help Service personnel

let

Service know which

each fault

the message does

cursor

SET display

has

1 of 2,

clear

6-6

Sorvall® Centrifuges

Troubleshooting

& Diagnostics

Screen

perform some maintenance routines, such

2 is

used

monitor system operation; install

assigning Key#'s (procedure

new

changing slow start/slow stop transition speed (procedure

the vacuum pump Table

6-1

describes what each field

to access

the

field.

oil

(page

Table

7-4).

6-1.

used

for and

what authorization (Key#)

Screen 2 Fields, DIAGNOSTICS

Field Purpose

Screen

User-1

User-1 User-2

monitor state

such

vacuum level

system operation,

and air

Maint-1 Install

temperature.

User-2

such vacuum

perform user diagnostic routines,

entering Key#s

and

changing

oil.

software;

add new

page 6-12),

rotors;

and

page

changing

required

Authorization Required

None

System Key#

and

6-9),

Maint-1

Instal 1 To

To enter Screen

Position

necessary, then enter

operation (Table

the

cursor under

view system accumulators. install software

press (DIAGNOSTICS) until

the

6-2) and

press (ENTER

To exit DIAGNOSTICS, press and press (ENTER

Selection

USER-1

2 3

6 7

9 10 11 12 13

14 15

O-).

Table

Vacuum Level (mbar)

Ctrlr Samp Temp

6-2.

Operation

Samp Temp Offset

Air Temp

Base Temp Jumper Temp Winding Temp

(deg

Measured Rotor Inertia Door Home Microswitch Door X Position

Door Closed Switch Door Lock Sensor URRID lst/2nd URR Y Value

Motor Direction

None

field

and add new

the "2"

you

want

to use and

rotors.

System Key#

indicator light above

press

(on off).

selection number that corresponds

O).

Press

to turn selection

DIAGNOSTICS, Screen

(deg

(on off) to

Selection

USER-1

turn selection

off,

enter selection number

Operations

Operation

(continued)

Tachometer Frequency-

Slow Transition Speed

Vacuum Transition Speed

Imbalance Threshold

Drive Relay-

Rail Enable

Rail Setting

Rail Volt Sense

Brake Enable Brake Setting

25 26

Drive Enable (Ctrlr)

Drive Enable (Host)

Bridge Enabled (Yes/No)

Bridge Chop (Half/Full)

Bridge Setting (Amps)

the key is lit.

Enter Key#,

to the

on.

(V)

(%)

desired

continued

—

6-7

Troubleshooting & Diagnostics

Sorvair Centrifuges

Selection

USER-1

USER-2

Table

6-2.

DIAGNOSTICS,

Operation

(continued)

Bridge Current Sense

HES Offset

Host State

NovRAM

Min

Max

Raw

Min

Max

Raw Base Temp

Max Base Temp

Raw Jumper Temp

Min Jumper Temp

Max Jumper Temp

Raw Winding Temp

Max Winding Temp

System Access Key#

Zonal Access Key#

Lock Access Key#

Program (1-20) Key#

Slow Transition Speed

Press

LCID Display Test

Vacuum System Override

Remote Baud Rate

Clear Faults

(U2) (U4)

Vac

Level (mbar)

Vac

Level (mbar)

Air

Temp

Air

Temp

Air

Temp

for

Oil

Battery Battery

(deg C)

(deg C) (deg C)

(deg

(deg C)

(deg C) (deg C)

Change

(A)

OK? OK?

Screen

Selection

MAINT-1

INSTALL

Operations (continued)

Serial Number (Year)

Serial Number (Unit)

Hours

Number

Hours

Hours Since

Number

Cycles Since

Drive Revs

w2dt (mantissa)

w2dt (exponent)

Number

Successful

No Match

Failed

Loose

Mismatched

Runs Ending with Faults

Last General Faults

Prior General Faults

Controller ROM Version

Host

Host ROM

Install Rotor

Install NovRAM

Install ROM

Operation

System

Drive

Drive Cycles

Vacuum Pump

Oil

Vacuum Cycles

Oil

(*10A6)

URR Cycles

URR

Cycles

Lid URR

URR

ROM (U5)

(U8)

(U5)

Time

Change

Drive

Cycles

Version Version

6-8

Screen

lists

the

for

use by

Field Purpose

Screen

Maint-2

Calib Seru-1

Serv-2

3 is

used

fields

DuPont

for

calibration

Screen

Table

3 and

other Service Personnel only.

6-3.

and

advanced troubleshooting

describes their purpose.

All of

and

the fields

Screen 3 Fields, DIAGNOSTICS

Authorization Required

Maint-2 Calib Seru-2

access diagnostic routines available

DuPont personnel only.

calibrate replacement subassemblies.

monitor system status

for

advanced

troubleshooting.

access diagnostic routines

advanced troubleshooting

and

for

repair.

repair. Table

this screen

No access

Service Personnel Service Personnel

Service Personnel

6-3

are

Sorvall® Centrifuges

Troubleshooting

& Diagnostics

How to Assign or Change a Key#

The centrifuge is shipped with a factory-set System Access Key# of "1234" that will

allow access to most fields that require a Key# (Screen 3 Service Diagnostics cannot be accessed by the System Access Key#).

If desired, you can change the System Access Key# and you can set different Key#s for various features, such as: the control panel Lock, Zonal, and up to twenty different Program Save Key#s. All Key#s must consist of four digits or less.

NOTE

Record all Key#s and keep them in a place where they will not be lost. If

Key#s are lost, call Du Pont for assistance.

System Access Key#

To change the System Access Key#:

Press (DIAGNOSTICS) until the "2" indicator light above the key is on and the display

looks as follows:

User-1 User-2 Maint-1 Install

Off Off Off Off

Press (ENTER O) to move the cursor to 1 User-2

Press (on off). The display will prompt you to enter a Key#:

User-1 User-2 Maint-1 Install

Off Key# Off Off

Input current |s-=isterc Recess Ke*#1 and press (ENTER O) ; display will look as follows:

User-2 <Ouride> Press Q or On to Exit

9 Off

Input 1 in the 1 User-21 field and press (ENTER O); cursor moves to |<Ovride)l.

User-2 (Quride) Systen Recess Key#

1 Off 1234-

CURRENT

ACCESS

KEY#

SYSTEM

6. Press (onoff) to turn | <ovride)[ on, then press (ENTERE»»).

Input the new | systen Recess Ke^#| (four digits or less). Press (ENTER D>).

User-2 <Ovride> Systen Recess Key#

1 On

8. Press (on off) to turn l«3vride)| off and press (ENTER O).

4444-

EXAMPLE NEW

SYSTEM

ACCESS

KEY#

6-9

Troubleshooting & Diagnostics

Sor vail® Centrifuges

9. Record

the

new System Access Key#.

To change other Key#s (i.e., Zonal, Lock, appropriate procedure that follows; System Access Key# procedure.

you are not

performing steps

10.

Input

in 1 User-21 field

going

10 &

assign

11 below.

and

press (ENTER D=>). The display will change back

User-1 User-2 Maint-1 Install

Off

Off Off Off

11.

Press

Zonal and Lock Access Key#s

Follow Steps 1 through

(PROGRAM)

exit

DIAGNOSTICS.

4 of

System Access Key# procedure, then:

NOTE

Program),

do not

repeat Steps 1 through

change other Key#s, exit DIAGNOSTICS

go to

Step

1 of the 4 of the

to:

change

Key# input

Press (ENTER

Press (on off)

Press (ENTER

Press (on off)

6. Press (ENTER

the

Zonal Key#, input

2 in the

| User-21 field. The display will change:

2 in the I user-21 field:

User-2 CQuride) Zonal Recess Key#

Off 1234

User—2 (Ouride) Lock

3 Off

O); the

O) and

cursor will move

turn kovride)

input

the

|<ovride>l.

on.

new Key# (four digits

less). Press (ENTER

User-2 <0vride) Lock Recess Key#

turn kovride)!

>) to

move cursor

2222

off.

to 1 User-21 field.

or to

change

O).

the

CURRENT ACCESS KEY#"S

EXAMPLE

NEW

LOCK KEY#

Lock

6-10

Sorvall® Centrifuges

NOTE

To assign or change Program Key#s, go to Step 1 of the following procedure,

but do not repeat Steps 1 through 4 of the System Access Key# procedure. If you are not going to assign Program Key#s, do steps 7 and 8 to exit DIAGNOSTICS.

Input £ in 1 User-21 and press (ENTER O). The display will change back to:

Troubleshooting

& Diagnostics

Press

(PROGRAM)

User-1

Off

exit

User-2

Off

DIAGNOSTICS.

Maint-1

Off

Install

Off

9. Record new Key#.

Program Key#(s)

A Key# is required to save a program and to make changes to an existing program,

but it is not required to recall a program.

A different Key# can be assigned to each of the 20 possible programs, if desired.

Follow Steps 1 through 4 of the System Access Key# procedure, then:

Input 4 in the 1 User-21 field and press (ENTER O).

User-2 < Task

Pro9ran<l-20) Key#

4 Off

Press (on off) to turn |< Task >1 on; display changes:

User-2 ( Task ) Enter Program Nunber

4 On 9

Press (ENTER

again.

User-2 < Task

Press (ENTER O) and input the Key# (four digits or less) that you want assigned to

the program (Program #01 is in the example above and below).

User-2 < Task > Key# for Progran #91

Press (ENTER o); cursor moves to |< Task )[. Press (on off) to turn [< Task )| off.

To assign or change Key#s for other Programs, repeat steps 2 through 5.

and input the desired program number (1-20). Press (ENTER O)

Key# for Pro9ran #91

4 On

1234

4 On 3333

CURRENT FOR

PROGRAM 1

EXAMPLE NEW

KEY# FOR

PROGRAM

NOTE

KEY#

6-11

Troubleshooting

Sorvall® Centrifuges

& Diagnostics

6. Press (ENTER O) to move cursor to | User-21 field. Input Q, and press (ENTER O). The display will change back to:

User-1

Off

Press

8. Record new Key#s.

(PROGRAM) to

exit

User-2

Off

DIAGNOSTICS.

Maint-1

Off

Install

Off

How to Change Slow Start/Slow Stop Transition Speed

The slow start/slow stop transition speed is approximately 800 rpm for all rotors except

the S-20 rotor, which has a transition speed of approximately 300 rpm; and the GS-3 rotor, which has a transition speed of approximately 650 rpm.

Transition speed can be changed to any speed from 100 rpm to 1000 rpm (the selected

speed will be rounded to the nearest number that is divisible by 4). When the transition speed is changed, it will affect the next run only (unless stored in a program); at the end of the run, it will automatically change back to the default speed of 800 rpm (300 rpm for the S-20 or 650 rpm for the GS-3). Anytime you want a run with a transition speed other than the default speed, you must change it prior to the run or store the transition speed as part of a saved program.

Prepare and install the rotor according to the instructions in the rotor manual. Close chamber door and select other desired run parameters, then change transition speed:

Press (DIAGNOSTICS) until the "2" indicator light above the key is on and the display

looks as follows:

User-1

Off

Press (ENTER O) to move the cursor to |user-2|.

Press (on off) The display will prompt you to enter a lKe*# |.

Input the Is^sten Recess Ke*#| and press (ENTER O).

User-2

Off

User-2 <0uride) Press

Off

5. Input §. in the [user-21 field and press (ENTER

Maint-1

Off

Q or On to

Install

Off

Exit

User-2 <0uride) Slow Transition Speed

5 Off 8Q0

6. Press (on off) to turn l«x»ride)l on, then press (ENTER

Slow

Transition

Speed

to move cursor to

6-12

Sorvall® Centrifuges Troubleshooting

& Diagnostics

Input desired transition speed (from 100 rpm to 1000 rpm).

CAUTION

Do not set transition speed at the critical speed (±100 rpm) of the rotor (see Table 4-1, Rotor Information, for critical speed). Slow start in the critical speed range can have a detrimental effect on centrifuge components.

8. Press (ENTER O) ; when you do, the cursor will move to 1 <0vride)|. Press (on off) to turn kOvride)! off.

9. Press (ENTER O) and the cursor will move to

10.

Input Q in|user-2land press (ENTER I>). The display will change back to:

|Us.er-21.

User-1 User-2 Maint-1 Install

Off

Off Off Off

11.

Press

12.

Press

(PROGRAM)

(START)

to exit DIAGNOSTICS.

to begin run.

NOTE

If the chamber door is opened after the transition speed has been changed, the transition speed will automatically change back to the default speed.

6-13/6-14

Sorvall® Centrifuges

Maintenance

Chapter 7

MAINTENANCE

This chapter describes routine maintenance procedures that are to be performed by the

user. Any further maintenance should be performed by a DuPont Service Representative or other qualified service personnel.

WARNING

Because the high voltage electrical circuits in this centrifuge can cause severe

electrical shock, untrained personnel must not attempt procedures that are not included in this manual. Failure to comply with this warning can result in personal injury.

If hazardous materials have been processed in the centrifuge, take all necessary precautions when cleaning or servicing the centrifuge to avoid personal exposure to contaminants.

To gain the most in safety, performance, and useful life from your centrifuge, it is necessary to maintain it as described in this chapter. Proper maintenance is a simple and inexpensive way to help prevent major failures. In addition to the maintenance described in this chapter, a Du Pont service contract, which includes a yearly Preventive Maintenance visit, is recommended to keep your centrifuge in top condition.

The following is a suggested maintenance schedule.

Table 7-1. Maintenance Schedule

Maintenance

Clean the rotor chamber

Wash the tapered spindle

Clean cabinet panels Clean the control panel Clean condenser coils Clean air inlet louvers Clean and inspect door seal

Daily or immediately after a spill Once a week Once a month Once a month Check monthly; clean as required Check monthly; clean as required Once a month or whenever vacuum

performance is poor

Frequency

Change vacuum pump oil and mist eliminator filter

Test RUN display lights and control panel indicators

Once a year or when the message

|+**

Tine to Service Uacuuri Punp ***|

appears in the SET display. Once a year or whenever numbers

displayed seem erroneous.

7-1

Maintenance Sorvall® Centrifuges

Cleaning

Rotor Chamber

The rotor chamber should be kept clean and wiped dry routinely to maintain

efficient cooling. Wash the chamber with a mild dishwashing liquid, then rinse and dry with a soft absorbent cloth.

Use 70% ethanol to disinfect the rotor chamber or a 2% glutaraldehyde solution to

sterilize it. For general radioactive decontamination, use a solution of equal parts of ethanol, 10% SDS, and water. Follow this with ethanol rinses then deionized water rinses.

radioactive waste containers.

Tapered Drive Spindle

Wipe the drive spindle with a soft cloth each time a rotor is to be installed to reduce the chance of the rotor sticking. Once a week, wash the drive spindle with warm water.

Dry with a soft absorbent cloth. Dispose of all wash solutions in proper

CAUTION

Chlorides are extremely harmful to aluminum alloy rotors and can cause stress corrosion cracking. Therefore, do not use chlorides to disinfect the chamber.

Cabinet

Clean the Noryl® cabinet panels with a household wax cleaner. Use a mild

noncdkaline

detergent and water to clean the top deck and the chamber door.

Control Panel

To clean the control panel, spray a small amount of a mild cleaning liquid (such as window cleaner) on a clean cloth and wipe the panel clean. DO NOT spray cleaners directly on the control panel.

Door Seal

The chamber door seal should be cleaned once a month. If the seal is not kept clean, it can result in an insufficient vacuum level during a vacuum run.

To clean the seal:

Open the chamber door all the way. Using a #1 phillips (or Reed-Prince)

screwdriver, loosen the quarter-turn fastener (turn counterclockwise) on the

front edge of the door (figure 7-1, view 1).

Close the chamber door halfway, then locate the hole in the center of the tray

assembly, underneath the door (figure 7-1, view 2). Insert your fingers approximately 13mm (1/2 inch) into the hole and pull forward on the silver seal tray until the hole is visible. Continue pulling tray out while pushing back on the door

handle until the door is fully open and the seal tray assembly is separated from

the door. Remove the sealing assembly.

7-2

Sorvall® Centrifuges

Inspect the top and bottom perimeter of the seal for signs of cracks, tears, or

abrasions. If damaged, replace the door seal assembly, Catalog No. 59235.

Wash the centrifuge deck and both sides of the door seal and tray with a mild

noncdkaline laboratory detergent and rinse well.

With the door fully open, carefully engage both corners of the tray in the door

tracks, then slide the seal tray assembly back into the door. Secure it in place

with the quarter-turn fastener.

Do not operate the centrifuge without the seal tray assembly in place. The assembly is part of the centrifuge containment system, which provides

protection in the event of a rotor failure.

Maintenance

WARNING

Figure 7-1. Door Seal Removal

7-3

Maintenance Sorvall® Centrifuges

Refrigeration System Condenser

To maintain the efficiency of the refrigeration system, keep the condenser fins (located

on the left side of the centrifuge) free of dust and dirt. Clean the fins with a brush or

vacuum cleaner at one to three month intervals. The fins will bend easily; be very

careful when cleaning them.

Air Louvers

To allow proper air flow through the centrifuge, the air louvers on the front cabinet panel should be kept free of dust and dirt. Clean the louvers with a brush or a vacuum

cleaner as needed.

Lubrication

All components are prelubricated and require no further lubrication. The refrigeration

unit is hermetically sealed and the ball bearings in the centrifuge motor are

permanently lubricated. Vacuum pump oil must be changed whenever the | »** Tirte to Service Uacuurc

message appears in the SET display or once a year, whichever occurs first.

Pump

*** |

Changing the Vacuum Pump Oil & Mist Eliminator Filter

The most common cause of vacuum pump failure or poor performance is contaminated

oil.

To ensure the efficiency of the vacuum system operation, the vacuum pump oil must be changed whenever the 1 *** Tine to Service Uacuurc Punt* ***! message appears in the SET display or once a year, whichever occurs first.

The | *** Tine to Service Uacuurc Punp *** 1 message will appear in the SET display on

the control panel to warn you that twenty or less vacuum runs* remain before the

vacuum will not operate. The oil should be changed as soon as possible after this

message appears. If the oil is not changed before the twenty vacuum runs are up, the {*** Uacuun Furcp Must Be Serviced »»*! message will appear in the display when you attempt to start the twenty-first vacuum run and an interlock will prevent further

vacuum operation until the oil is changed. A DuPont Oil Change Kit, Catalog No. 12870, is required for this procedure. The kit

includes the items needed to change the oil: a bottle of vacuum pump oil, a mist eliminator filter, a plastic exhaust port blocking tube, a piece of tape, and a funnel.

NOTE

If you are not using the DuPont Oil Change Kit, you must use oil that meets the same specifications as the oil supplied in the kit. The specifications for the oil are given in the Appendix of this manual with a list of additional items required.

* A vacuum run occurs when a Sorvall9 Si/praspeed® rotor is operated above its maximum air speed. Maximum air speed is equal

to maximum speed of the rotor in an RC-5C Centrifuge. See rotor instruction manual.

7-4

Sorvall® Centrifuges Maintenance

Refer

Figure

7-2 for

parts location

and

change

the

vacuum pump

oil:

NOTE

best

change

warm

Turn

Peel

over

Turn

of panel

Remove right access panel from behind control console

panel

and

will pump

the

centrifuge power

the

back

off the

the

left-hand inlet port

the two

and

fasteners

out,

then down,

lifting

up.

the oil

out

while

easier.

ON and

piece

tape supplied

completely seal

the

bottom

and

remove panel from centrifuge.

necessary, strike

it is

open

warm, since

the

chamber door.

the Oil

the

lower front cabinet panel. Pull bottom

end

it is

less viscous when

Change

port.

panel lightly

Kit,

then stick

pressing

pop

in on

panel

off.

the

tape

end

WARNING

Leave chamber door open while access panel avoid

the

and

possibility

the

door track behind

pinching your hand

the

console.

off.

This

and

fingers between

necessary

the

door

Loosen knob

6. Remove mist eliminator filter

Install

the eliminator. Reinstall full turns after

8. Remove entire length

cap

mist eliminator. Then, remove

and

screen. Discard filter

exhaust port blocking tube (supplied

the cap and

you

first feel resistance.

vacuum pump drain line from channel

knob assembly

and

the kit)

panel.

9. Remove drain line tubing clamp, then securely position bottle having a capacity

one

liter

more.

NOTE

10.

Make sure the access panel

Turn

the

Press (DIAGNOSTICS) until

appears

the

drain line tubing

and

vacuum pump

the

display.

User-1

Off

where

on:

the

User-2

not

kinked, particularly near

was

crushed

"2"

indicator light above

Maint-1

Off

the

tube clamp.

cap and

and

tighten

end

the key

Install

Off

knob assembly.

keep

the

screen.

the

stem

it:

turn

the

front access

drain line

in a waste

the top

is on

and 1 User-2

the

knob

mist

two

Press

prompt

(ENTER

you

User-1

E>)

Off

enter

move

the

a Key#:

User-2 Key#

cursor

to User-2

Maint-1

Off

Press (on off);

Install

Off

7-5

Maintenance

Sorvall® Centrifuges

LEFT-HAND

INLET PORT

KNOB-

MIST ELIMINATOR CAP

MIST ELIMINATOR SCREEN

EXHAUST PORT BLOCKING

TUBE

MIST ELIMINATOR FILTER

STEM OF

MIST

ELIMINATOR

RIGHT ACCESS PANEL

QUARTER-TURN

FASTENERS

7-6

FRONT

PANEL

Figure

7-2. Oil

DRAIN LINE

TUBING CLAMP

DRAIN

LINE

CLAMP

PLACE

Change Parts Identification

RIGHT SIDE INLET PORT PLUG

ONE

LITER

WASTE

BOTTLE

CLAMP REMOVED

Sorvall® Centrifuges

c. Input the System Access Key# and press (ENTER D>).

User-2 (Quride) Press 9 or On to Exit

d. Input £ and press (ENTER O).

User-2 < Task ) Press On for Oil Change

e. Press (on off) and press (ENTER O).

Usei—2 < Task ) Enter 1 to Run PUNP

f. Input 1 and press (ENTER O).

User-2 < Task ) Enter Q Nhen Enpty

Maintenance

9 Off

6 Off

6 On 9

6 On

NOTE

If vacuum pump turns off within 30 seconds after it is first turned on, check to make sure the piece of tape inside chamber completely seals inlet port and that the inlet port plug on left side of centrifuge is firmly in place.

11.

Run the pump until air comes through the drain line and froth begins to appear in

the waste container; this indicates that the pump is empty. Immediately press

(ENTER O) , input Q. and press (ENTER O) again to turn pump OFF and avoid

damaging the pump.

CAUTION

Do not run the pump after it is empty. To do so can damage the pump.

NOTE

The pump will turn off automatically after 4 minutes. If the pump is not

empty after the 4 minutes, press (ENTER O), input 1 and press (ENTER E>)

again to continue running the pump.

12.

Press (onoff) to turn |< Task )| off.

13.

Press (ENTER O) to move the cursor under | User-21 • Input 0 and press (ENTER O») .

14.

Press (PROGRAM) to exit DIAGNOSTICS.

15.

Wipe oil from drain line tubing and clamp.

16.

Position tubing clamp in place on drain line. Close clamp and tighten it securely to

seal the drain line. Store the line back in the front access panel.

17.

Unscrew the knob on the mist eliminator cap and remove cap and knob assembly.

7-7

Maintenance Sorvall® Centrifuges

18.

Remove exhaust port blocking tube from the stem of the mist eliminator. Do not

install mist eliminator filter yet.

19.

Unscrew the nut from the oil fill line fitting (located in front of the mist eliminator)

and remove the fill line plug assembly.

20.

Insert the funnel (supplied in the kit) in the oil fill line fitting. Make sure the funnel

is in as far as it will go, then secure it in place by tightening the locking nut.

21.

Open the bottle of oil and slowly pour entire contents (675 ml) into funnel, being

careful not to overflow the oil in the funnel.

22.

Properly dispose of the collected waste oil.

WARNING

Disposal of the waste oil must conform to all local, state, and federal standards.

23.

Loosen the locking nut on the funnel and remove the funnel.

24.

Reinstall the fill line plug assembly in the oil fill line fitting as far as it will go and

tighten the nut; the nut should be finger tight.

25.

Position the new mist eliminator filter in place, then install the mist eliminator

screen over the filter.

26.

Install mist eliminator cap and knob assembly over the mist eliminator screen,

making sure screen is fully seated inside cap; tighten the assembly in place by turning the knob on the cap.

27.

Reinstall the access panel behind the control console.

28.

Reinstall the front cabinet panel.

29.

Remove piece of tape from inside the chamber. The centrifuge is ready for use.

Emergency Sample Recovery

WARNING

When the main power shuts off, the brake will not operate. Wait until the rotor stops before using the mechanical override. Reaching into the rotor

chamber while the rotor is spinning can cause personal injury.

a. Nonvacuum Runs

Wait until the rotor has coasted to a stop. When the rotor stops, insert a straightened paperclip in the door interlock override hole and push down (figure 7-3). Open chamber door.

Vacuum Runs

If a power failure occurs, vacuum will not release automatically and you will not be able to open the chamber door. The chamber will maintain a partial vacuum and the rotor

will safely coast to a stop. The vacuum must then be released manually.

7-8

Sorvall® Centrifuges

To release vacuum:

Maintenance

STRAIGHTENED PAPER CLIP

DOOR INTERLOCK OVERRIDE HOLE

CHAMBER DOOR

Figure 7-3. Door Interlock Override

Wait until the rotor has coasted to a stop, then turn the power switch OFF.

When the rotor stops, unplug the centrifuge.

Locate the vacuum release solenoid valve (figure 7-4). Insert a screwdriver

through the grille on left side of back panel, and turn vacuum release screw fully

clockwise (approximately 4 to 5 turns). Air will begin to enter chamber.

VACUUM

RELEASE

SOLENOID

VALVE

VACUUM

RELEASE

SCREW

Figure 7-4. Vacuum Release Solenoid Valve Location

7-9

Maintenance

When the air

stop),

5. Insert a straightened paperclip in the door interlock override hole (figure 7-3). Open

turn the vacuum release solenoid valve screw fully counterclockwise.

door.

the chamber has reached atmospheric pressure (hissing noise will

So r

vail® Centrifuges

Test

Periodically test the control panel RUN display and indicators to verify that the lights are functional:

RUN Display Lights

Turn the centrifuge power ON.

Press (DIAGNOSTICS) until the

looks

follows:

"2"

Control Panel Indicators

indicator light above the key

on and the display

User-1 User-2 Maint-1 Install

Off

Off Off Off

Press (ENTER

Press (on off). The display will prompt you

5. Input the System Access Key# and press (ENTER O).

6. Input

7 in

O) to

User-2 <Qvride> Press

the I User-21 field and press (ENTER t>).

move the cursor

Off

to 1 User-21.

enter

9 or On to

Exit

User-2 <Ouride) LCID Display Test

7 Off S

7. Press (onoff). When (on off)

• the number

•

all

• the minus temperature symbol will light

• the cursor indicator lights will If a number other than "8" appears

come on, call for Service.

8. Press (on off).

9. Press (ENTER

indicator lights on the control panel will come on;

pressed:

"8"

O) .

will light

each space across the RUN display;

all

come on.

the display or

the RUN display; and

any of the other lights do not

7-10

Sorvall® Centrifuges Maintenance

10.

Input 0 under | User-21 and press

(ENTER

O). The display will change back to:

User-1 User-2 Maini-1 Install

Off

Off Off Off

11.

Press (PROGRAM) to exit DIAGNOSTICS.

Parts Ordering Information

To order replacement parts: in the United States, telephone toll-free (800) 551-2121. Outside the United States, contact local distributor or agent. Be sure to provide a description of the part, centrifuge model, and centrifuge serial number.

Service Decontamination Policy

WARNING

Because of the characteristics of the samples likely to be processed, biological or radioactive contamination may occur. Always be aware of this possibility and take normal precautions. Use appropriate decontamination procedures should exposure occur.

If a centrifuge or rotor that has been used with radioactive or pathogenic material requires servicing by DuPont personnel, either at the customer's laboratory or at a DuPont facility, comply with the following procedure to ensure the safety of all personnel:

• Clean the centrifuge and/or rotor to be serviced of all encrusted material and decontaminate it prior to servicing by the DuPont representative. There must be

no radioactivity detectable by survey equipment.

• Complete and attach Decontamination Information Certificate (Sorvall® Products

Form No. IPDP-59 or E53603) to the centrifuge or rotor.

If a centrifuge or rotor to be serviced does not have a Decontamination Information Certificate attached and, in DuPont's opinion presents a potential radioactive or biological hazard, the DuPont representative will not service the equipment until proper decontamination and certification is complete. If Du Pont receives a centrifuge or rotor at its Service facilities which, in its opinion, is a radioactive or biological hazard, the sender will be contacted for instructions as to the dispositon of equipment. Disposition costs will be borne by the sender.

Decontamination Information Certificates are included with these instructions.

Additional certificates are available from the local Technical or Service Representative.

In the event these certificates are not available, a written statement certifying that the

7-11

Maintenance Sorvall® Centrifuges

unit has been properly decontaminated and outlining the procedures used will be acceptable.

NOTE

The Service Representative will note on a Customer Service Repair Report if

decontamination was required and, if so, what the contaminant was and what

procedure was used. If no decontamination was required, it will be so stated.

7-12

Vacuum Pump Oil Specifications

If you are not using DuPont Oil Change Kit (Cat. No. 12870) when changing the vacuum pump oil, you must use oil that meets the specifications below.

Oil Specifications:

Molecular distilled general purpose hydrocarbon mechanical pump fluid,

675 ml.

Ultimate pressure @ 25°C: Viscosity <g> 38°C: Specific Gravity @ 22°C: Flash Point: Fire Point: Auto Ignition:

These properties are typical of Cambridge Mill Products #VB-X.

Also Required:

Mist Eliminator Filter, Technolab #ED5-IL Plastic Tape, minimum width 50 mm (2 inch); 3M #471 or equivalent

6x105Torr 375 SUS 80 CST

0.88 251 °C 279°C

449°C

Warranty

WARRANTY

E. I. du Pont de Nemours and Company makes no warranty of any kind, expressed or implied, except as stated in this warranty policy.

The Sorvall® RC-28S Supraspeed® Centrifuge is warranted to be free from defects in material and workmanship for a period of one year from the date of delivery. Du Pont will repair or replace and return free of charge any part which is returned to its factory within said period, transportation prepaid by user, and which is found upon inspection to have been defective in materials or workmanship. This

warranty does not apply to any damage to any instrument resulting from: normal

wear and tear; misuse; abuse; use of electrical currents or circuits other than those specified on the plate affixed to the instrument; accident; negligence; failure to follow operating instructions; or use of any rotor other than a Sorvall® rotor intended for use in this instrument.

Du Pont reserves the right to change, alter, modify or improve any of its instruments without any obligation whatsoever to make corresponding changes to any instrument previously sold or shipped.

THE FOREGOING OBLIGATIONS ARE IN LIEU OF ALL OTHER OBLIGATIONS AND LIABILITIES INCLUDING NEGLIGENCE AND ALL WARRANTIES, OF MERCHANT-

ABILITY OR OTHERWISE, EXPRESSED OR IMPLIED IN FACT OR BY LAW, AND

STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND BUYER'S EXCLUSIVE REMEDY FOR ANY CLAIM OR DAMAGES IN CONNECTION WITH THE SALE OR FURNISHING OF GOODS OR PARTS, THEIR DESIGN, SUITABILITY FOR USE,

INSTALLATION OR OPERATION. DU PONT WILL IN NO EVENT BE LIABLE FOR

ANY SPECIAL OR CONSEQUENTIAL DAMAGES WHATSOEVER, AND OUR

LIABILITY UNDER NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH LIABILITY IS CLAIMED.

Glossary

Cursor

Default

Value

Display

Message

Enter

Entry

Fault Fault

Message

Field

Input

A movable indicator in the SET display screens that shows

position for data entry.

The choice made by the computer system when no explicit

choice is made by the operator.

A message that appears in the display that prompts the operator

to perform a specific operation or informs the operator of an

operating condition.

To transfer an input to the computer by pressing either the

right (ENTER O) or the left (ENTER <Q cursor key. An input that has been transferred to the computer. A centrifuge malfunction.

Message produced by the computer program that indicates a fault

has occurred.

One item of information within a screen; cursor must be positioned in field to make an entry.

The data to be transferred to the computer by pressing the

keypad.

Integrator

K Factor

Key Number

RCF

Run Display

Screen

Set Display

Keeps track of the accumulating centrifugal effect during a run, including acceleration and deceleration.

A value used to estimate run time at a specific speed to move

particles in suspension (assumes at 20°C) from the rmin of the

rotor to rmax of the rotor. The lower the K Factor, the more efficient the rotor is in sedimenting particles.

A security number that provides user authorization to access

protected data.

(g force) The force applied to a suspension of particles during centrifugation relative to the force that the earth's gravity would have on them.

The upper display on the control panel which shows actual time, Jo^dt, temperature, and speed values.

A group of fields visible in the SET display selected by pressing

either the PROGRAM, CALCULATE, or DIAGNOSTICS key.

Hie lower display on the control panel that changes according to

the screen selected.

Transition

Speed

The speed at which the rotor changes from slow acceleration or

deceleration to maximum acceleration or deceleration when Slow Start or Slow Stop is used.

Index

+/- Key, 3-3

Brake,

Cabinet, Cleaning, 7-2

CALCULATE,

Calculator, 1-4, 3-9, Chamber, Rotor, 1-4 Cleaning,

CLEAR Key, 3-3 Compartment Loads, 4-12 Control Panel, Control Panel, Display & Indicator Test, 7-10 Control Panel Lock, 1-3, 3-8, 4-11 Decontamination Policy, 7-11 Diagnostics, 1-4, DIAGNOSTICS Key, 3-9,

Dimensions, Centrifuge, 1-2, 2-1

Display

Door Interlock Override, 7-8 Drive System, 1-3, 1-5 Electrical Requirements, 1-2, 2-2

Emergency Sample Recovery, 7-8 ENTER Keys, 3-3 Faults, Filter, Mist Eliminator, 7-4 HOLD Key, 3-3 Hold Run, 4-8 Hr:Min Field, 3-4 Indicators, Test, 7-10 Inspection, 2-1

Installation, 2-1 Jco2dt Calculation, 5-1 Jco2dtRun, 1-3, 3-7, 4-9 Integrator, 1-3, 3-7-3-8, 4-9 K Factor, Calculation, 5-2 Key Number, 1-4, 4-6, 6-10 Keys

Keypad, 3-3 Leveling, 2-2 Location, Centrifuge, 2-1 Lock, Control Panel, 1-3, 3-8, 4-11

3-5

Display Screens, 3-9, 5-1

Key, 3-9,

Air Inlet Louvers, 7-4

Cabinet, 7-2

Control Panel, 7-2

Condenser Coils, 7-4 Door Seal, 7-2 Drive Spindle, 7-2 Rotor Chamber, 7-2

Messages, Run, 3-1

Screens, CALCULATE, 3-9, 5-1 Screens, PROGRAM,

Set, 3-1 Test, 7-10

CALCULATE, 3-9,

CLEAR, 3-3 DIAGNOSTICS, 3-9, PROGRAM, HOLD, START, 3-3 STOP,

5-1-5-2

7-1-7-3

3-1-3-3

6-5-6-9

6-1-6-5

3-3-3-9

6-6

5-1-5-2

6-5-6-9

3-3-3-9

3-3

Lubrication, 7-4 Maintenance, 7-1 Mass,

Centrifuge, 1-2

Normal Run, 4-1

Oil,

Vacuum Pump, 7-4 ON/OFF Power, 4-1 On/Off Key, 3-3

Operator's Guide, 1-4 Ordering Information, 7-11 Override, Door Interlock, 7-8 Overtemperature, 1-2, 3-5 Parts,

Ordering, 7-11 Power, ON/OFF, 4-1 Precool/Preheat, 4-12 PROGRAM Key, Program Recall, 3-8 Program Save, 3-9

Programmed Run, 1-4, 3-8, 4-10

RCF

Calculation, Field, 3-6 Run, 1-3, 3-6, 4-5 Set, 3-6

Recall, Program, 3-8 Refrigeration System, 1-3, 1-5 Rotor

Field, 3-4 Information, 4-4 Management System, 1-2, 1-3, 1-5, 3-4

Run Display, 3-1 Safety Information, ill Sample Recovery, 7-8 Save,

Program, 3-9 Screens, Display. See Display Service, Decontamination Policy, 7-11 Set Display, 3-1

Slow Start/Slow Stop, 1-3, 3-6-3-7, 4-3 Speed

Calculation,

Control, 1-2 Field, 3-4 Reducing, 4-12 Set, 1-2, 3-4

Spin-Right® Rotor Management

System, 1-2,

START Key, 3-3

STOP Key, 3-3 Temp/Max Field, 3-5 Temperature, Ambient, 1-2, 2-2 Temperature, Control, 1-3 Temperature, Maximum, 3-5 Temperature, Set, 1-2, 3-5 Time,

Calculation, 5-1

Time,

Display, 1-2, 3-4

Time,

Set, 1-2, 3-4

Transition Speed, 3-6-3-7, 6-13

Vacuum, 1-2, 1-3, 1-6 Vacuum, Bleed, 7-8 Vacuum Pump Oil, 7-4 Warranty. See Appendix Zonal Field, 3-7 Zonal Run, 1-3, 3-7, 4-7

3-3-3-9

5-1-5-2

1-3-1-5,

3-4

PROGRAM

LOG

PROGRAM

LOG

PROGRAM

LOG

PROGRAM

LOG

Rate.

Date.

Speed

Temp_

not

turn ZONAL

LOG

Date.

PROGRAM

Operator

Rotor

Brake

RCF. Slow Start

Slow Stop

Notes

PROGRAM RECALL will

but

_Min_

DoFF Zonal* Speed

will set speed & other parameters. but

PROGRAM

Operator

PROGRAM

Operator

Rotor

Brake

RCF.

Slow Start

SlowStopj^pRate.

Notes

On, * PROGRAM RECALL will

Min

Zonal*Speed.

Rate.

will set speed & other parameters. but

PROGRAM

PROGRAM*

Operator

Date.

Speed_

Temp_

not

turn ZONAL

LOG

Date.

PROGRAM*

Operator

Rotor

°C

On, * PROGRAM RECALL will

.Min. ION

Brake

y^F Zonal* Speed

RCF Jco2cfr Slow Start R2£l Rate

Slow Stop

Notes

will set speed & other parameters. but

Rate

PROGRAM

PROGRAM*

Operator

Date.

Speed

Temp_

not

Date.

turn ZONAL

LOG

PROGRAM#.

Operator

Rotor

Min

Brake

RCF.

Slow Start

Slow Stop

Notes

On, * PROGRAM RECALL will

will set speed & other parameters.

Zonal* Speed

Rate.

PROGRAM

PROGRAM*

Operator

Date.

Speed

Temp_

not

Date.

turn ZONAL

LOG

On,

Rotor

Brake

Min

Speed

Temp

Zonal* Speed

RCF

SlowStartj^pRate.

SlowStop0°^FRate.

Notes

PROGRAM RECALL will

but

will set speed & other parameters. but

PROGRAM

Operator

Rotor

Hr.

Brake

Min

HoFF

Zonal*

not

Date.

Speed

Temp__

Speed

turn ZONAL

LOG

Rotor

Min.

Brake

RCF_

Slow Start

Slow Stop

Notes

On, * PROGRAM RECALL will

will set speed & other parameters. but

Zonal*

_ Wdt

Rate.

PROGRAM

PROGRAM*.

Operator

Rotor

_°C Hr.

Min

Brake

DoFF Zonal*Speed.

Speed

Temp_

Speed

not

turn ZONAL

LOG

Date.

Speed,

Temp_

Rotor

Brake

RCF_

Slow Start

SlowStopgg^pRate.

Notes.

On, * PROGRAM RECALL will

PROGRAM*.

Operator

Rotor

Brake

Speed

Min.

Temp.

Zonal*Speed.

Rate

g^p

not

will set speed & other parameters. but

PROGRAM

turn ZONAL

LOG

Date.

Speed

Min

0gFF Zonal*

Temp_

Speed

On, * PROGRAM RECALL will

Rotor

Min.

Brake

DoFF Zonal* Speed

RCF

Slow Start

Slow Stop

Notes

will set speed & other parameters.

Rate.

Rate

PROGRAM

PROGRAM*.

Operator

Rotor

Min

Brake

Zonal*Speed

Speed,

Temp_

not

turn ZONAL

LOG

Date.

Speed_

Temp_

On,

°C

RCF Slow Start

Slow Stop

Notes

PROGRAM RECALL will but

will set speed & other parameters. but

Rate

not

turn ZONAL

RCF_ Slow Start Qg

SlowStop^pRate

Notes

On, * PROGRAM RECALL will

will set speed & other parameters. but

FJF

Rate

not

turn ZONAL

RCF.

Slow Start

Slow Stop

Notes

On, * PROGRAM RECALL will

will set speed & other parameters. but

Rate

not

turn ZONAL

RCF_

SlowStarty°^FRate.

SlowStop^pRate.

Notes

On, * PROGRAM RECALL will

will set speed & other parameters.

not

turn ZONAL

On,

DECONTAMINATION CERTIFICATE

Instructions on the reverse of this card must be completed

before field service

to the Du Pont/Sorvall service facility.

NAME DEPARTMENT INSTITUTION __ ADDRESS

CITY STATE ZIP.

INSTRUMENT SERIAL NUMBER

ROTOR - .-. SERIAL NUMBER PART PART NUMBER CONTAMINATE USED _ DECONTAMINATION CERTIFIED BY _

DATE DECONTAMINATED.

or the

return

of the

instrument

part

DECONTAMINATION CERTIFICATE

Instructions on the reverse of this card must be completed before field service or the return of the instrument or part to the Du Pont/Sorvall service facility.

NAME DEPARTMENT INSTITUTION __„_ ADDRESS

CITY ________ STATE ZIP.

INSTRUMENT

ROTOR. SERIAL NUMBER PART PART NUMBER CONTAMINATE USED. DECONTAMINATION CERTIFIED BY. DATE DECONTAMINATED

„„_-_____

SERIAL NUMBER

DECONTAMINATION CERTIFICATE

Instructions on the reverse of this card must be completed before field service or the return of the instrument or part to the Du Pont/Sorvall service facility.

NAME DEPARTMENT INSTITUTION ADDRESS

CITY STATE ZIP. INSTRUMENT SERIAL NUMBER ROTOR SERIAL NUMBER

PART PART NUMBER CONTAMINATE USED DECONTAMINATION CERTIFIED BY DATE DECONTAMINATED

INSTRUCTIONS

When an instrument that has been used with radioactive or pathogenic

material requires servicing by Du Pont personnel either at the customer's laboratory or at Du Pont facilities, the following procedure must be

complied with to insure safety of our personnel:

The instrument or part to be serviced shall be cleaned of all blood

and other encrusted material and decontaminated prior to servicing by our representative. No radioactivity shall be detectable by survey equipment.

A Decontamination Information Certificate (Sorvall Instruments

Form No. IPDP-59) shall be completed and attached to the instrument

or part

If an instrument or part to be serviced does not have a Decontamination

Information Certificate attached to it, and, in our opinion, the instrument or part presents a potential radioactive or biological hazard, our representative will not service the equipment until proper decontamination and

INSTRUCTIONS

When an instrument that has been used with radioactive or pathogenic material requires servicing by Du Pont personnel either at the customer's laboratory or at Du Pont facilities, the following procedure must be complied with to insure safety of our personnel:

1 The instrument or part to be serviced shall be cleaned of all blood

and other encrusted material and decontaminated prior to servicing by our representative. No radioactivity shall be detectable by survey equipment.

A Decontamination Information Certificate (Sorvall Instruments

Form No. IPDP-59) shall be completed and attached to the instrument or part.

If an instrument or part to be serviced does not have a Decontamination Information Certificate attached to it. and, in our opinion, the instrument or

part presents a potential radioactive or biological hazard, our representative will not service the equipment until proper decontamination and

certification has been completed. If an instrument is received at our Service facilities and, in our opinion, is a radioactive or biological hazard, the sender will be contacted for instructions as to disposition of the equipment. Disposition costs will be borne by the sender.

Decontamination Information Certificates are included with these Operation Instructions. Additional certificates are available from your local technical or customer service representative. In the event these certificates are not available, a written statement certifying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable.

NOTE

Service representatives will indicate on a Customer Service

Repair Report if decontamination was required, and if so, what the contaminate was and what procedure was used. If no decontamination was required, it should be so stated.

certification has been completed. If an instrument is received at our

Service facilities and, in our opinion, is a radioactive or biological hazard, the sender will be contacted for instructions as to disposition of the equipment. Disposition costs will be borne by the sender.

Decontamination Information Certificates are included with these Operation Instructions Additional certificates are available from your local technical or customer service representative. In the event these certificates are not available, a written statement certifying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable

NOTE

Service representatives will indicate on a Customer Service

Repair Report if decontamination was required, and if so, what the contaminate was and what procedure was used. If no decontamination was required, it should be so stated.

INSTRUCTIONS

The instrument or part to be serviced shall be cleaned of all blood

and other encrusted material and decontaminated prior to servicing by our representative. No radioactivity shall be detectable by survey equipment.

A Decontamination Information Certificate (Sorvall Instruments

Form No. IPDP-59) shall be completed and attached to the instrument

or part.

If an instrument or part to be serviced does not have a Decontamination Information Certificate attached to it, and, in our opinion, the instrument or part presents a potential radioactive or biological hazard, our representa-

tive will not service the equipment until proper decontamination and

certification has been completed. If an instrument is received at our

Service facilities and, in our opinion, is a radioactive or biological hazard,

the sender will be contacted for instructions as to disposition of the

equipment. Disposition costs will be borne by the sender.

NOTE

Service representatives will indicate on a Customer Service Repair Report if decontamination was required, and if so, what the contaminate was and what procedure was used. If no decontamination was required, it should be so stated.

IPDP-59

Sorvall RC-28S User manual

Specifications and Main Features

Frequently Asked Questions

User Manual