Sorvall Cell Washer Service manual

Service
Parts list Updated Apr '05
Manual
SORVALC
SORVALL®
Newtown,
U.S.A.
Connecticut
Cellwasher
CELL WASHING
SYSTEM
2
SORVALL
PN 04624-5 Issued February
Centrifuges
1994
Cellwasher
2
Service
This
manual is
SORVALL®lnstrumentS
guide
a
for
service of the
SORVALL®
Data herein has been of the the obtained, liability. Infringe
This includes information from
This
by must not attempt
NOTES,
important
instrument.
and
specified
publication
manual
to the high electrical
Due
critical
capabilities
otherwise,
This
upon, any
service manual
instrument to instrument.
service
DuPont.
CAUTIONS, and
Cellwasher
verified and validated
If
the
instrument
herein,
DuPont
process patents.
intended
is
regarding
intended
is
of the
any
instructions:
is
procedures
WARNINGS
does not
2
and is believed
or procedures
confirmation
not
guarantee
potential
in
to
by
this
the
a license
as a service
significant design
for
use only
within
Cell
are
of
their
results and
operate
While
aid.
changes,
service
this
in
service manual.
text of
Washing
adequate used for validity and suitability
under,
the
specific
personnel centrifuge,
this
manual are used
System
purposes
assumes no
nor a
manual
designs
who have been
untrained
for the intended
and above
over
should
obligation
recommendation to
is
kept current
may
individuals
to
emphasize
still
trained
use
be
and
vary
or
WARNING A
CAUTION
NOTE
Ili^^^^^^^^^^^^^^^^^^^^^^^^^^
When
characteristics
I?''lew^^^ltt'lii^^
rotor can protect
^['licit^
^'eiiilitiil^^
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Warning result in environment.
A
Caution informs
damage
A
Note highlights essential
using radioactive,
informs
personal
of
equipment.
of the
you
!
material
from the
the
operator
injury,
the
'
toxic, or pathogenic
affect the operator's
operator
information.
'
^^^NG';'^'^'6'!;''1:^;":,!^
the
and
particles dispersed
of
hazard or an
a
of
unsafe
an
material,
hazards associated
into
unsafe
health,
practice
the
that could
be
with
air.
practice
or
aware
To
contaminate
of
In the
it.
protect
that could
result in
all
.:
.
the
ii
Sorvall®
Instruments
Cellwashcr
2
Service
Table
Paragraph Page
Section
1-1.
1-2.
1-3.
1-4.
2-1.
2-2.
2-3.
2-4.
2-5.
2-6.
Intended
Warnings
Service
DESCRIPTION,
Description
Cellwasher
Installation
Cellwasher
Emergency
Condensed
APPENDED:
Users
and Cautions
Decontamination
Responsibility
of the Cellwasher
2
Specifications
Information.
2
Operation
Sample
Operating
Condensed
of
INTRODUCTION
I.
....................................
..............................1-1
.............................
Section
INSTALLATION
.............................
..............................
Recovery
Instructions
Contents
Policy.
2.
2
...........................
..........................
Operating
.......................
and
........................
.....................
Instructions
OPERATION
...........
1-1
1-1
1-2
2-1
2-1
2-2
2-10
2-17
2-18 2-19s
3-1.
3-2.
3-3.
4-1.
4-2.
4-3.
4-4.
4-5.
4-6.
4-7.
4-8.
4-9.
4-10.
Section
Inspection
Tubing Replacement
Cellwasher
APPENDED:
Controls
Flexible
Cabinet
Air Flow
Motor
Safety Latch
Saline
Flow
Detector
Flow Control
Motor Antirotation Clutch
and
2
Preventive Maintenance Procedure Preventive Maintenance Checklist
Section
.........................................
Drive
...........................................
Pump
Mounting
.........................................
.........................................
......................................
System
Clamp
3.
Cleaning
MECHANICAL THEORY
4.
.....................................
MAINTENANCE
.............................
................................
.............................
................................
................................
............................
...........
...........
3-1
3-5
3-5 3-9
4-1
4-1
4-1
4-1
4-1
4-1
4-2
4-2
4-2
4-2
Rev.
in
11/90
Cellwashcr
2
Service
Sorvall®
Instruments
Paragraph
5-1.
5-2.
5-3.
5-4.
5-5.
6-1.
6-2.
6-3.
6-4.
Table
Section
System
Touch Switch
Interface
Microcomputer
Electronics
Cellwasher
Compatibility
Cellwasher
Glossary
Description
of
PC
Board
Module
Section
2
2
of Blood
Contents
5.
Panel.
PC
Board
Application
Testing
Washing
Banking Terms
(continued)
ELECTRICAL THEORY
.................................
................................
.................................
............................
.................................
APPLICATIONS
6.
.............................
...............................
Cycle
..........................
......................
Page
5-1
5-31
5-31
5-37
5-50
6-1
6-1
6-2
6-6
8-1.
8-2.
8-3.
8-4.
8-5.
8-6.
8-7.
8-8.
8-9.
8-10.
8-11.
Section
Base
Cabinet
Front
Power
Pump
Pump
Flow
Motor
Interface Printed
Microcomputer
Fuse
8.
Plate
Removal...................................
Panel Removal
Switch
Replacement.................................
Motor
Detector
Replacement.................................
Board
Board
Replacement................................
Replacement................................
Replacement..................................
Section
REPAIR
Removal.................................
7.
and
Replacement
Replacement
Replacement...........................
Circuit
Printed Circuit
TROUBLESHOOTING
REPLACEMENT PROCEDURES
................................
...........................
............................
8-1
8-1
8-2
8-3
8-3
8-3
8-4
8-4
8-4
8-5
8-5
IV
Rev.
11/90
Son/all®
Instruments
Cellwasher
2
Service
Table
Paragraph Page
9-1.
9-2.
9-3. 9-4.
9-5. 9-6.
9-7.
Tubing
Collector Seal
Retainer Lid
Latch
Kit
(PN
Saline Triac-Interface Printed Circuit Board
Replacement
Adapter Clip
of
Contents
Section
Replacement
Ring Replacement
Assembly Replacement
12792)....................................
Shield Kit
Section
9.
Replacement
(PN 12811)
Kit
Installation Kit (PN
10.
(continued)
KIT INSTRUCTIONS
Kit
12816)
(PN
ILLUSTRATED PARTS LIST
(PN
04632)
Kit
(PN
Kit
(PN
..........................
.........................
04330)
....................
04353)
12850)
.................
...............
...............
9-2
9-9
9-12
9-14
9-19
9-20
9-23
Figure
2-1.
2-2.
2-3.
2-4.
2-5.
3-1.
3-2.
5-1.
5-2.
5-3.
5-4.
5-5.
5-6.
List of Illustrations
Cellwasher
Stainless Steel
Distributor Installation
Cellwasher
Location
Exploded
8702302
(SN
Exploded
8702301
(SN
System
Wiring
Microcomputer
Schematic,
Triac-Interface
SN 8503865
Schematic,
Revision
2
2
of Mechanical
View,
View,
Schematic,
Diagram,
Microcomputer
Triac-Interface
2,
Tubing Diagram
Rotating
Controls
Collecting
and higher)
Collecting
and
PC
PC
and
SN 8503865
and Indicators
lower)
Cellwasher
Cellwasher
Board
Board (PN
higher)
.........................
Bowl Installation
..............................
Override
Assembly
Ring
...........................
Assembly
Ring
............................
2
2
.........................
............................
PC
Board
04834
............................
PC
Board
and higher)
...................
.......................
.......................
...................
Revision
(PN
...................
................
04537
Page
2-4
2-2
2-9
2-10
2-18
3-3
3-5
5-3
5-5
5-7
5-11
0,
5-16
5-17
Rev.
v
11/90
Cellwasher
2
Service
SORVALL®lnstrumentS
List of
Figure
5-10.
5-11. Cellwasher 5-12. 5-13. 5-14. 5-15. 5-16. 5-17. 5-18. 5-19. 5-20. 5-21.
5-22. 7-1.
7-2. 9-1. 9-2. 9-3. Adjustable 9-4. 9-5.
9-6. Cabinet Machine
9-7. Adhesive
9-8. Installing
10-1. Cellwasher
10-2. 10-3. Drive Motor
Schematic,
Revision
Indicator
Schematic, Interface Interface Microcomputer Microcomputer Creating Digital Time Starting in Manual (High
Starting from Starting in Auto Location of
Location of Tubing Adjustable
CW-1
Cellwasher 2
Chassis and
1,
Current
PC PC
New
Collecting Ring Assembly
Location
an
Illustrations
PC
Onterface
SN 8503864
2
Automatic Wash
Path
Touch
Board, Board,
Display
Decant Lift
Tubing Clamp Tubing Clamp
2
Assembly
Switch Panel
Board Scan
Input/Output
Check
Mode, Logic
Coil
Plate
Parts
Collecting Ring Assembly Exploded
Drawing
.......................................
Adapter Clip
Assembly
Component
Board (PN
and below)
...................................
Test Set-Up Test Set-Up
Input/Output
Routine
Segments
or
Mode, Logic
and
Ring
.................................
Location
Location
.................................
.................................
.................................
....................................
Assembly
.....................................
Formats
Low)
Flow Chart
Drive
04537
Sequence
............................
#1
..........................
#2
..........................
Configuration
..............................
.............................
Mode, Flow
Ring
............................
..........................
Exploded
...........................
(continued)
........................
......................
........................
Logic
Chart
......................
.......................
View
Flow
...................
.................
View
..............
Chart
...........
.........
Page
.5-25
.5-30
.5-31 .5-33 .5-35 .5r36 .5-38 .5-39 .5-41 .5-42 .5-47 .5-48 .5-49 .7-7 .7-7 .9-2 .9-6 .9-8 .9-10 .9-11
.9-17 .9-20 .9-24
10-5 10-11 10-15
List
Table
2-1. Parts 2-2. 5-1. 5-2.
5-3. Triac 5-4.
5-5. Automatic Wash 5-6. 5-7.
vi
Cellwasher
Component Component
Component
Microcomputer Electronics
Location:
(PN
04834,
PC
(PN
04536, Revision
Board Component
Tubing Diagram
2
Controls and Identification, Identification,
Revision
Identification,
Sequence
Scan
Control
0,
2,
Map
System
of Tables
Indicators
Microcomputer Triac-Interface
SN 8503865
Identification
Interface
SN 8503864
................................
.................................
................................
............................
........................
and
PC
Board
and below)
PC
Board
PC
Board
higher)
.....................
..............
...............
...............
Page
.2-4 .2-9
.5-15 .5-19
.5-23
.5-30 .5-40 .5-43
5-8
Rev.
4/96
SORVALL®
Instruments
Cellwashcr
2
Service
List
of
Table
6-1. 6-2.
Applications Applications
Troubleshooting Chart: Troubleshooting
6-3. Applications Troubleshooting
Strength 7-1. 7-2.
7-3.
Troubleshooting Troubleshooting
Troubleshooting 7-4. Troubleshooting 7-5.
10-1.
Troubleshooting
Suggested 10-2. CeUwasher 10-3. Chassis and 10-4. Drive
.............................................
Spare
2
Assembly
Component Assembly
Motor Assembly
Chart: Chart: Chart: Chart: Chart:
Parts,
Tables
Chart: False Positive Results Chart:
Drive Motor Power
Saline Decant Lid
..............................
Fill
..............................
Latch
CeUwasher
....................................
.....................................
(continued)
False
Negative
Inconsistent Reaction
.........................
...........................
...........................
2
.........................
...........................
Results
........
........
Page
6-3
.6-4
.6-5
.7-2
.7-3 .7-4
7-5
.7-6
10-2
10-3
10-7
10-13
Rev.
n/90
vii/viii
SORVALL®
Instruments
Cellwasher
2
Service
This System. procedures; information; replacements; parts.
1-1. Intended
This
familiar
calibrations. procedures operator,
involving
service
1-2.
manual
manual has been written for qualified service
Warnings
is
It
mechanical and
with
contained in
the
electrical
personnel.
a
contains
troubleshooting
a
and
Users
factory
While the
replacement
and Cautions
service
an
Section
guide for
descriptive
illustrated
methods
circuitry)
1.
electrical
guide;
for
descriptive
this
and calibration
manual are
INTRODUCTION
the
Sorvall
information;
theories
field
parts
performing repairs,
should be
list
of
procedures for repairs and
information
useful to
procedures (especially
performed
Cellwasher
preventive
operation; applications
for
ordering replacement
2
personnel
adjustments
and
the
only by
Cellwashing maintenance
operating
cellwasher
qualified
who
are and
those
Warnings
are expected
read them
1-3. Service Decontamination
————————————————————————
If
an
requires servicing
laboratory
to
ensure
and cautions
to
before servicing an instrument.
Either biological instrument likely to possibility and decontamination
instrument or
at
or
the
safety
Clean encrusted the by
the
representative.
survey
be familiar with their
and/or rotor can occur
be
rotor
DuPont
a
instrument
material,
equipment.
appear
processed. Always
take
procedures
DuPont
by
of
throughout
Policy
WARNING
or radioactive
normal
that
facility, DuPont
has been
and/or
and decontaminate
There must
precautions.
should
personnel,
personnel:
comply
the
manual.
meaning
————————————————————————
contamination
because of
be
exposure
with the
be
no
with
either at
to
it
radioactivity detectable
used
rotor
Service
(see
aware
Use
biohazardous material
be serviced of
prior
the
appropriate
occur.
following
page
samples
of
the
to servicing
of
personnel
ii)
the
this
customer's
procedure
and
to
all
by
Rev.
11/90
Attach (SORVALL
rotor.
a completed
Instruments Form
Decontamination Information Certificate
No.
IPDP-59)
the
to
instrument
or
1-1
Cellwasher
If Decontamination Information opinion presents a not
is
complete.
facilities be contacted for instructions Disposition costs
2
Service
the instrument
service
the
which,
equipment
If
potential
DuPont
in
will be
and/or
DuPont's
until
receives
rotor
biohazard,
borne
Certificate
proper
an
opinion
as
to be serviced
attached,
the
DuPont representative
decontamination and
instrument
to by
is
the
the
biohazard/
a
disposition
sender.
SORVALL®
does not have
and in
certification
rotor
or
at its Service
the
the
of
sender
equipment.
Instruments
a
DuPont's
will
will
Decontamination Information
Service
used
Service
what the
If
of
performing
warranty,
Pont
Additional
written statement
a
has been
will
Representative
decontamination
no
Responsibility
the
of
DuPont
de Nemours and
instructions. Technical
not
are and/or rotor procedures
1-4.
Whenever
an
individual instrument
available,
The
Service Repair if
used. stated.
employee
E. I. kind, expressed or implied, except as policy.
or
so,
service
Du
Certificates
certificates
Representative. In
decontaminated and outlining
NOTE
will
decontamination
was was
is
authorized DuPont
is
assuming
follows:
as
Company
be
Report
the
which
properly
acceptable.
contaminant
instrument
or
an
service
if
is
are
the
certifying
note
and
required,
attempted
makes
are available
event these
was required,
what
the
stated
included with these
the local
certificates
instrument
and
was
be
so
other than
voiding
of
warranty
on
no
that the
the
procedure
it
will
anyone
by
representative,
risk of
warranty
this
in
from
Customer
the
the the
any
1.2
Sorvall®
The Rotor workmanship for a period delivery. return free within said is or
from has been altered nor negligence, of on
DuPont any
corresponding changes shipped.
are
found workmanship.
use;
to
any
electric the
plate
reserves
of
Cellwasher
warranted
DuPont
of
period,
upon
its
inspection
it
does not
instrument
failure
currents or
affixed
instruments
charge
the
to
will,
transportation prepaid
This
anyone
by
to
right to
2
Cell
be free
of
at
any part
to have been warranty apply
other
which
to the instrument, misuse
follow
to
has been
circuits
without
any
Washing
from defects in materials and
one
its
which is
to
operating
change,
(1)
option,
does not include normal any
than
damaged
other than those specified
instrument
instrument and
year
repair or replace
returned to
defective in materials
instrument
an
employee
instructions,
alter, modify or improve
any
obligation
previously
from
by
through
user,
or part
the
date
its
factory
and
of
DuPont,
accident,
or abuse.
to
sold
the
DA-12
which
wear
which
make
and
use
of
or
11/90
Rev.
SORVALL®
Instruments
Cellwasher
2
Service
THE FOREGOING LIABILITIES
MERCHANTABILITY
LAW, EXCLUSIVE SALE OR FOR
USE, LIABLE FOR ANY UNDER FOR WHICH LIABILITY
If
there
warranty,
OBLIGATIONS ARE IN LIEU OF ALL OTHER OBLIGATIONS INCLUDING
OR OTHERWISE,
AND STATE
OUR ENTIRE
REMEDY FOR ANY
FURNISHING
OF
INSTALLATION
SPECIAL OR
NO CIRCUMSTANCES WILL
IS CLAIMED.
are
contact
questions concerning
any
the
nearest
NEGLIGENCE
EXPRESSED
AND ALL
OR IMPLIED IN
AND EXCLUSIVE LIABILITY
CLAIM OR DAMAGES GOODS OR
OR
PARTS/
OPERATION. DUPONT WILL IN
CONSEQUENTIAL
EXCEED THE
CONTRACT PRICE FOR
the
representative of
IN CONNECTION
THEIR
DAMAGES,
effect of
Sorvallo
WARRANTIES/ OF
FACT OR BY
AND
DESIGN,
SUITABILITY NO EVENT BE
AND
OUR LIABILITY
service
Instruments.
WITH
THE
BUYER'S
GOODS
on
AND
THE
the
1-3/1-4
SORVALL®
Instruments
Cellwasher
2
Service
Section
This section describes the Sorvall®
fications,
the
instrument.
2-1.
The
the
are
panel.
The Cellwasher
with
features
warning
cells and
Description
SORVALL®
Coomb's
contained in
sensor-touch controls and
installation
Cellwasher
Procedure automatically.
2 is
include
and
eliminates
an
agitate
DESCRIPTION,
2.
information, and
the
of
one cabinet, with
microprocessor-controlled, featuring a
saline
a
Cellwasher
2
is
designed
detect
cycle
manual agitation.
INSTALLATION
Cellwasher
instructions
2
to
perform
The
centrifuge and
the
operating
digital timer for
a
with
that
system
ensures complete resuspension
OPERATION
and
2
and provides speci¬
for operating
the
washing phase
saline
controls
the
wash
audible low-saline
an
on
front
cycle.
the
of
pump
front
panel
Other
of
2-2. Cellwasher
Dimensions:
Width
Depth
Height,
Height,
Mass
Electrical Requirements*:
Motor
Speed**
Low
High
Decant
(Weight)
Type
............
............
for
lid
lid
closed
open
60
Hz models:
2
Specifications
31.8 cm
35.6
36.8 cm
.
57.2 cm
16.4
115
V
230
V
3
speed
1150
3500
600
cm
kg (36.0
+
+
to
to
rpm
(12.5
(14.0
(14.5
(22.5
10%,
10%,
induction
1200
3600
in)
in)
in)
in)
Ib)
60
or
50
rpm
rpm
Hz,
Hz,
5A
2A
Speed**
Low
High
Decant
for
...................
..................
................
50
Hz
models:
1450
2950
600
rpm
1500
to
3000
to
rpm
rpm
(continued)
2-1
Cellwasher
2
Service
SORVALL®
Instruments
Decibel
Length
Automatic
Manual
Tube
Tube
Diameter
Length
....................................
of Modes:
Sizes:
Material:
................................
...................................
.................................
...................................
.............................
67 Db
Total
seconds
High
up timed; indefinite
in
12
10
75
High
as
to
HOLD
mm
mm
mm
strength
Pyrex®
or
cycle
999
+
or +
+ 2
low,
0.09
0.09
or
time
seconds
mm
optional,
mm
mm*
glass,
Corex®
80
if
if
such
Use
specifications given
specifications are
the
2-3.
Place the
drain,
factors should be
Installation
Location
a.
only
rotor
injury
or
Allow
saline
Allow
surface for
could result.
Cellwasher
waste
a
a
15
pump
tubes
and
container
61
distributor
Information
2
considered
cm (6
and tubing.
(24 inch)
cm
the
which
on a
inch)
lid
WARNING
are
above.
used,
to
test results
could
sturdy/
receive decanted
when
clearance
clearance
to
open.
guaranteed
If
tubes outside these
be
damaged,
level bench
selecting a
behind
above
to be
will be
or
saline.
location:
the
the
within
and
table
instrument
tabletop
affected,
personal
near a sink,
The following
the
the
for
bench
or
2-2
"Adapter
Clips (PN 04330) are required
when
using
10
mm x 75
mm
tubes in
the DA-12
Rotor.
Rev.
11/93
SORVALL®
Instruments
Drainage
drain
drain,
the
in
tubing must
sink, or
receptacle.
2
is accomplished
Cellwasher
extend downward from
waste
so
2
Service
the
Ccllwasher
by gravity,
the instrument to the
If
the saline
recheck
paragraph
b.
The
preferred location for
above instrument
To obtain
saline
Cooling air
sides of the
obstructed.
the
2-4,
Electrical
supply
supply
saline
b.
Requirements
the
the saline
level.
best
to
is
drawn
base.
reservoir
volume
results,
the
cellwasher.
CAUTION
into the Cellwasher
Be
sure
is
priming
by
minimize tubing length
that the
moved
from its
the
supply
2
entrances
system as explained
is either
from
all
four
not
are
original location,
at
from the
or
in
The
power
inserts
c.
be
115
specified
Tubing
Cellwasher
must
as
—————————————————————
The
instrument,
tubing
of the instrument
cord for
into
connected to
The
not,
possibility
in
a receptacle at
+
V
10%,
on
receptacle
the
Installation
but
the peristaltic
the
equipment
2
not
the
Cellwasher 2 has
the
60
Hz,
rating plate
used should
of electrical
is
shipped
installed in
and
proceed
the
appropriate
5A
WARNING
must be
with
pump,
back of
or
on
be
grounded
shock.
the
the
unwrap
as
follows
universal
a
the
instrument.
power
230
the
—————————————————————
3-wire
a
tubing already
peristaltic
the
supply:
+
V
10%,
back of
to earth to avoid
bundle of
(refer
the
system.
pump.
to
keyed
50
instrument.
If
connected to the
tubing
figure
The other
Hz,
it
the
install
To
at the back
plug
2.5A
is
2-1):
that
end
the
2-3
Cellwashcr
2
Service
SORVALL®
Instruments
Remove the four
1.
and
remove
the
pump
clean and
Turn the
2.
11
o'clock
Without
3.
rollers (the end
Pull
4.
up
into the
knurled thumbscrews from
the
front
roller
assembly.)
move freely.
roller assembly
position.
turning
the
with the connector
the
on
pump
two ends
housing
half of
the
Make
that
so
rollers, place
of the
around
pump
the
sure
pump
that
of the rollers
one
the
should
tubing,
rollers.
the
peristaltic
housing. (Do
the
pump
be
tubing
on
and
the left
fit
pump
pump
not
remove
rollers are
is in the
around
the
side)
the
tubing
.
2-4
Figure
2-1. Cellwasher
2
Tubing
Diagram
Rev.
4/96
SORVALL®
Instruments
Cellwasher
2
Service
Table 2-1.
Item Description
I*
2
3
4
5
6
7
8
9
10
not
Do
as
this
use
a sharp
could
Parts
(key
Overflow Tubing
Vent
Pump
Flow Tubing
Discharge
Supply
Drain
Pump
Y-Connector
Adjustable Tubing Clamp
puncture
Tubing
Tubing
Connector
object
Tubing
Tubing
Location:
to figure
15
25
(to
Tubing
NOTE
to
the
tubing.
15
cm
cm
nozzle)
(to
121
fit the
2-1)
(6
(10
20
pump)
cm (48
Tubing Diagram
cm
cm (8
inches)
(6
inches)
inches) long
30
cm
inches) long
60
cm
inches)
pump
tubing
long
long
(12 inches)
(24 inches)
long
in
long
long
place
With
5.
pump
pump
Make
6.
gap the
Secure
7.
tighten.
Check the
8.
assembly
smoothly
Install the flow control
9.
a.
the
pump
housing.
housing
sure
indicates that the
front
Cut
supply
on
that
half
using
pump
shaft
without
length
a
tubing.
the left side of
tubing
Stretch
in
place
the
halves
of the
the
four thumbscrews
tubing installation
counterclockwise
pinching).
of
tubing
Attach
in
the
to
housing
place,
pump
the
the
ends
eliminate
of the
tubing
valve
60
supply
pump
replace
cm
of the
and
as
(24
tubing
slack in
housing are evenly
is
repeat steps
(removed
by
using
follows:
inches)
tubing to
housing.
the
pinched;
pliers
in
turning
long
front
while
the
tubing.
if
3
step 1)
(should rotate
to
the
pump
half
fitting
so,
through
the
be
of
mated.
remove
and hand
roller
used
connector
the
the
A
6.
as
Rev.
*
the
In
event of saline residua] in
normal
operating
11/93
conditions,
the
saline
rotor chamber
will
through
exit
(e.g.
the
from overfill
drain
tubing, not through
of
tubes or
tube
breakage) saline
the overflow
tubing.
will
exit through
this
tube.
Under
2-5
Cellwasher
2
Service
SORVALL®
Instruments
Place
b.
and
insert
reservoir.
Connect the
c.
housing to the inlet
10.Into
peristaltic
(with
11. Holding
strap over
outer
to
secured.
12
.Make sure
or
accomplished
possible and
upward travel to
the
discharge
strap over
lock
waste
the
valve
the
adjustable tubing
pump,
the
Y-Connector in
the
in
place.
the
drain
receptacle
by gravity.
make sure
over
end
pump
position
tubing
Y-Connector/discharge
the
You
tubing flows downward
result in
the
supply
of the
tubing from
inner
will
since
the
port
attached).
Use
a
supply
clamp
the
place
strap
hear
drainage
the
tubing
"trap"
of the
bottom
with
a
shortest
line
tubing
the
located to
and
"click"
is
for collected waste
at a convenient
into
right
flow detector.
of the
leg
hand,
one
tubing. Then,
push
in the
positioned
on
when
to an
length
a
side
the left
the
open sink, drain,
Cellwasher
of drain
saline
of the
Y-Connector
fold
outer
the
so
point
supply
of the
the
fold
clamp
tubing
there is
fluid.
pump
inner
the
strap
is
2 is
no
13.Ideally,
not become immersed
is
a possibility
waste fluid,
the
waste
Y-Connector in the drain line
a
for discharged
The
the line due
vent
to
result
If the
location, recheck
explained in
as
14.If
necessary, use
the
properly.
Tubing
the
receptacle
Y-Connector
the
in
improperly
saline
drain tubing should be
in
collected waste
that the
the drain
cut
and insert the
saline.
prevents
to submerged tubing
drain tubing
supply
Kit
reservoir is
the saline
paragraph
additional
to
supply
NOTE
decanted
drain
tubing at
pressure
installing
by
volume
2-4,
tubing and connectors from
saline
positioned
fluid.
tubing
when
or
samples.
moved from
b.
will
a
Y-Connector.
using
from
air
blockage.
priming
by
and discharge waste
become immersed in
convenient point
building
Y-Connector
a
that
so
However,
Always
waste container
a
up
Failure
its
original
the
system
if
can
in
it
there
insert
can
near
2-6
"s"-4/^
SORVALL®
Instruments
WARNING
Ccllwasher
2
Service
Saline
azide
directly
as
your
Cellwasher
All
Routine
3) are
of
DA-12
d.
Open
1.
————————————————————
solution
a
as
down
the
sodium azide reacts
saline
saline solutions have
cleaning
essential to
the
Cellwasher
Dual Angle
the
preservative.
supplier
2
into
lid
from
the
and maintenance
Rotor and Distributor Installation
of
some manufacturers contains sodium
If this
drain, explosive
with the
before discharging saline from
a drain.
CAUTION
long
safe and
WARNING
the
ensure
2.
Cellwasher
solution is
azide salts
plumbing.
term
(as
2.
corrosive effects.
described
efficient
————————————
discharged
form
may
Check with
in
Section
operation
the
Sorvall®
All
1984
which
bowl assemblies
be removed
to do
injury.
a
There
rotating
balancing
ance
2.
were supplied
required annual
so can
Replace
stainless steel
may
of the stainless
Position
motor drive shaft. Align
the
slot
down
the
the
rubber
Cellwashers manufactured
from
bowl
purposes only
the
in the
drive
service and discarded immediately. Failure
result
be
one or more
assembly (PN 12796).
stainless steel
shaft until the two drive
drive
with
are more
in
the
plastic
rotating
drive
ring.
plastic rotating
a
replacement.
than
bowl
NOTE
holes in
and
the
Press
of
rotating
will
damage
steel rotating
shaft
prior
All
plastic rotating
one
equipment
rotating
two drive
(see
the
year
(1)
and/or personal
bowl
assembly (PN 12796).
the
stainless steel
These holes
effect the
not
bowl.
bowl
pins
figure 2-2). Slide
pins
bowl down
to
February
bowl
old and
assembly
assembly
should
are
perform¬
assembly over
in the bowl with
in the bowl
firmly.
with
for
the
the
bowl
engage
2-7
Cellwasher
2
Service
SORVALL®
Instruments
Slide the
3. into
Figure
DA-12
the
rotating bowl.
2-2.
Rotor
Stainless
Bowl Installation
over
Steel
the drive shaft
Rotating
MOTOR SHAFT
SLOT
ALIGNMENT
STAIN
LESS ROTATING ASSEMBLY
DRIVE
and
DRIVE
PINS
lower
STEEL
BOWL
it
gently
Hold the
4. until
the
rotor
Place
5.
the distributor until
the
rotor.
seated
If
the
Rotor,
the
to
Cellwasher 2:
Make
are
and
with all three
figure
edge
the
drive
drops
the distributor
the
on
stainless steel
distributor
and
instrument
sure
engaged
that the
2-3).
Press
the
of the
rotor.
drive
in
distributor
distributor
pins
down
the
the
will
rubber
rotating
the
in
onto
over
its
distributor
are
pins
the
the
three pins
CAUTION
rotating
not installed
result. Before
in the
drive
is
pins
bowl
rotating bowl
rotating bowl.
rotor knob
firmly seated
engaged
and turn
engage
down
bowl
rotating
ring
until
assembly,
properly,
and
in the
engage
(see
operating
bowl
in
the
on
the
rotor slowly
the
figure
three holes
it is
damage
assembly
the
rotor,
the
rotor
2-3)
completely
DA-12
the
rotor
(see
rotor
and
Turn
.
in
2-8
SORVALL®
Instruments
DISTRIBUTOR
CANNULA
DISTRIBUTOR PIN
ROTOR KNOB
DUAL ANGLE ROTOR
Cellwasher
2
Service
Figure
e.
The
using
Rotor
Cellwasher
less than
Loading
and Balancing:
2
can process
full
a
compartments to ensure
volume
at
of saline delivered
the
locations
without
basin and drained off.
Use
only
tubes
specifications given
these specifications
the
injury
rotor
and
could result.
2-3.
Distributor Installation
any
complement of
that
the
through
tubes, saline
which
are guaranteed
in
are
used,
distributor
balanced load
tubes, place
rotor
the distributor will
WARNING
paragraph
test
could be
is
will
results
up
symmetrically
be
to
2-2.
damaged,
to twelve
the tubes in
centrifuged
within the
be
If
tubes outside
will
be
and
balanced.
affected,
personal
be
tubes.
opposing
the
into
When
The
same/¬
the
Rev.
11/93
2-9
Cellwasher
2
Service
SORVALL®
Instruments
2-4.
All
switch,
controls
Power
right hand
"1"
Switching
the
Table
Figure
Cellwasher
Controls and Indicators
a.
controls
are
register commands visually
the
to
corner
indicates
the
memory.
2-2
describes the
2-4
shows
2
Operation
and indicators
located
instrument
of the
ON while
power
their
off
for
the
on
is
front
controlled
cellwasher, beneath
the
symbol
erases
any
controls
locations
the
Cellwasher
panel keyboard.
and/or
a
by
"0"
indicates
manually
and indicators
the
on
except
2/
The sensor-touch
audibly.
switch located in
the
keyboard.
the OFF
programmed spin
the front
on
keyboard.
the
power
the
lower
The
symbol
position.
times from
panel.
2-10
*LED,
light emitting
or
Figure
diode,
20 19
2-4.
refers to
18
Cellwasher 2
small
the
amber light
16
adjacent
17
Controls
the
to
15
panel
14 13
and
button.
Indicators
SORVALL®
Instruments
Cellwasher
2
Service
Item
Table 2-2.
Name Acceptance Signal
AUTO
HIGH
LOW
4
CHECK
LED*
accompanied
LED*
accompanied
LED*
accompanied
LED*
accompanied
Cellwasher
lights
lights
lights
lights
(key
up,
by
up, by
up, by
up, by one beep,
2
to figure
one
one
one
Controls and Indicators
2-4)
Selects
beep.
beep.
beep.
mode for automatic
wash
Selects HIGH
run
of time
Selects LOW
run
time
When
only,
end
wash
verification of
dure.
cycle.
for
for
selected.
in AUTO
of
Function
AUTO
duration
selected.
duration
stops
any step
cycle
run
speed
speed
mode
at
to
the
in
proce¬
of
the
allow
during
LOW
mode
3
number
se¬
AUTO,
mode
mode
duration
or
mode.
4
in
CYCLE
CYCLE
7
TIME
TIME
START
Panel key.illuminates,
digital
accompanied
Digital
accompanied
beep.
LED*
accompanied
cycle display
time
lights
by
by
up,
by
one
display,
one
one
beep,
beep.
Selects
wash
the
Displays
of wash
lected in the AUTO
Displays
selected
HIGH
seconds.
HIGH
In
only/selects
of
centrifugation time
in
seconds.
Starts
selected.
cycles
AUTO
or
the
1, 2,
mode.
the
cycles
the time
for
LOW
or
'LED,
light
or
emitting diode,
refers
the small amber light adjacent
to
the
to
panel
button.
2-11
Cellwasher
2
Service
SORVALL®
Instruments
Table 2-2.
Item Name
10
11
12
STOP
PRIME
AG
Cellwasher
(key
Acceptance
LED*
accompanied
Single
Single
lights
to figure
beep.
beep.
2
Controls and
2-4, continued)
Signal Function
up,
one
by
beep,
Indicators
Stops
progress.
HIGH,
the
program
to
conditions
start of the
Primes the
6
seconds.
Agitates the
5
seconds
cell
button.
the
or
spin
In
LOW
will
pump
to
in
AUTO,
mode,
return
the
at
run.
for
rotor for
resuspend
13
14
15
16
17 ALARM
CLEAR
ALARM
OFF
STEP
HOLD
ON
Single
Single
Single
lights
beep.
beep.
beep,
up.
ALARM
HIGH
In
only,
display
can be
In AUTO
turns
In AUTO
passes one step
time
for each
of
the
HIGH
In
sets the
spin indefinitely.
In AUTO
activates
sounds
end of the
or
clears digital
that
so
reset.
mode
off the
mode
the
in
button.
at
push
or
centrifuge
mode
an
the
wash
LOW
alarm
cycle.
LOW
only,
only,
only,
alarm.
mode
time
at
cycle
mode,
that
by¬
a
to
2-12
18
*LED,
SALINE SALINE lights
light emitting
or
diode,
refers to the
small
light adjacent to the
amber
up.
panel
button.
Lights
supply
during
when
up
is
interrupted
AUTO fill.
saline
SORVALL®
Instruments
Cellwasher
2
Service
Item Name
19
20
b.
The
installation,
is
LID
ALARM ALARM
Priming
Cellwasher
changed.
Table 2-2.
LED*
the
2
and
(key
Acceptance
System
must be
whenever
Cellwasher
to figure
lights up.**
lights
up.
primed using
the
2
Controls and
2-4,
Signal Function
saline
continued)
the
supply
Indicators
Allows
mechanism to be
at the
a
run.
In
lights
the
the
following
reservoir or
the lid latch
end
AUTO
up
alarm
of the
end
of
mode
to indicate
will
procedure
the
opened
sound
run.
tubing
only/
at
at
1.
2.
3.
When
described
Open
Select:
Holding
lid,
the
the
press:
saline flow
in
paragraph
lid
MODE
graduated cylinder
a
MODE
pressing
by
CYCLE
CYCLE TIME CMD
NOTE
is
interrupted, prime
2-4
TIME CMD
NOTE
SALINE
d,
the LID
under the saline
button,
alarm.
the
Cellwasher
dispenser
2
as
in the
*LED, "The
If
command.
saline
or light
Cellwasher
emitting
2
bubbles remain in the
air
Air bubbles
the
diode,
can only
in
refers
be
to the
opened
test
small amber
the
when
tubes.
lid LED
may
light
adjacent
is illuminated.
tubing, repeat
cause
to the
panel button.
uneven
distribution
the PRIME
of
2-13
Cellwasher
2
Service
SORVALL®
Instruments
The
4
Once
.
and
volumes
the
increase
Repeat steps
5.
If
the
fill
washing,
AUTO
c.
basic
1.
Mode
operating sequence
Press
bubbles
air
repeat step
54
are
volume
or
saline
volume
check saline volume
the LID
ml
is
not
decrease
3
supply
should be
button
3
and
have
to
for
correct,
been
determine
4 until the
reservoir
removed, empty
12
the flow of
rechecked.
in
to
tubes,
mm
NOTE
the AUTO
open
saline volume
43
rotate
saline.
correct volume
is
moved
To ensure
daily.
mode
the
lid.
the
ml for
the flow
or
is
graduated
(recommended
10
mm
control valve
is obtained.
replaced,
proper
follows:
as
tubes)
the
cell
cylinder
fill
If
.
to
2.
3.
4.
5.
6.
7.
8.
To ensure
first
2 will
Fill
Install
2-3,
Close
Select:
For an
Press
the
At
signal.
Once
rotor,
d
and
the lid.
MODE
audible
START to
end
lid LED is
the
proper
run.
stop.
is
generally considered adequate
tubes
e.
CYCLE
signal at
begin
the
of
At the
Examine the level
run,
fill,
end
distributor
and
the
press
press
of the
CMD
the
run.
NOTE
TIME
illuminated, press
of the
end
ALARM OFF
CHECK
fill
step,
of
saline
as explained
to turn
after
if:
run,
LID
beginning
the
to
Cellwasher
in
each tube.
in
press
off the
open
paragraph
ALARM
audible
the lid.
the
ON.
2-14
SORVALL®
Instruments
the level of
metal bands
saline
holding
in all tubes
the
tubes,
is visible
Cellwasher
above
the
2
Service
d.
the difference
is less than
START
alarm:
supply
lid
inch
to continue
this
is
prime
(press
a-
graduated cylinder under
the CMD
pressed.
7/16
Press
SALINE
saline
the
problem,
1. Open
Holding
2.
lid/ press
The SALINE ALARM will
has been
between maximum
0.7
10
for
alarm
interrupted. To
the Cellwasher
LID
ml/
mm
will
button).
(command)
not shut
or
tubes.
the
NOTE
fill
3/8 inch for
run.
sound during
deactivate
2
PRIME
off until the PRIME
as
described
the
button.
and minimum
12
mm
the filling
the
alarm and
on
saline
fill
tubes and
step
the
next
dispenser
button
if
correct
in the
the
page.
During
3.
The
tubing.
tubing.
3,
To
cycle
AUTO
Press
CHECK
any the CHECK
automatically
lid
lid
START
SALINE
If
Clean
Maintenance).
ensure adequate
the
at
operation,
button
step
then be
may
and
alarm
the
of the
press
to
continue
may
saline reservoir is
or replace
washing,
completion
the following features
-
the
automatic wash
cycle
button
during
stop
START to continue
at
opened.
the
NOTE
also
tubing
of the
(i.e.,
the
wash
indicate
if
perform one
cycles
fill,
that
end
After
step.
of the
checking
cycle
broken
adequate,
necessary
cycle
the
spin,
step
run.
additional
in
The
in
progress.
may
can
the
progress.
or
(see
also be
decant)
Cellwasher
in
obstructed
check
Section
be
checked after
by pressing
progress
tubes,
wash
the
used:
close
and
2
will
the
the
2-15
Cellwashcr
2
Service
STEP
it
the
the STEP button
bypass.
will
sequence
button
restart at the
-
ahead
if
the AUTO
(to
once
mode
beginning
avoid refilling tubes for
for each
is
interrupted
of the
step
programmed
in the
by
cycle
SORVALL®
STOP
a
run.
instance)
you
Instruments
command,
To
press
wish
move
to
STOP
by
lid
begin
HIGH
e.
Press
1.
Install
2.
2-3,
3.
pressing
and
Select:
button
may
the
LOW
the LID
rotor, tubes/
d
and
-
the
the STOP
be
opened.
programmed cycle over again
Speed Mode
button to
e.
MODE CYCLE
in
run
When the instrument is
progress
button.
open
and distributor
TIME CMD
1.999|
SECONDS
NOTE
When
the
can
the LID light
unless
lid.
as explained
STAR-H
be
stopped
restarted,
steps
immediately
goes
are
in
on/
it
bypassed.
paragraph
the
will
The
operating
of
or
or LOW,
in the
4.
5.
Depending on
instrument
at
spin
that
until the
After the
automatically for
will count down in
At the
low
time
in the HIGH
mode
the time selected for
digitial
end
to
speeds
programmed
until
power
start
of the
run
instrument,
for
or
a new
is switched
time
button is
5
run/
conditions
a
time is
display.
seconds before
seconds.
press
few seconds after
into the Cellwasher
LOW
mode remains
selected
off.
the
previous run
pressed/
LID
to
NOTE
and component
the
rotor
may
in the
the
by
After
the
open
the
pressing
will
agitation
run begins.
the
variability from
still
be
lid
is
2
operator
lid.
spinning
opened.
when
memory
HIGH
appear
will
The
occur
time
2-16
SORVALL®
Instruments
CeUwasher
2
Service
2-5.
If the
malfunction
open. the
case
and is
During
6.
also
AGITATE
2
Emergency
main
A mechanical override is
of
intended
HIGH
be
spinning/
HOLD
indefinite
an
STOP
power
while the
an
emergency.
used:
-
after
button
Sample
for
LOW
or
-
pressing
will
selecting
is
Recovery
shuts off
rotor
emergency
speed operation,
the
provide
spin period.
pressed.
because
is
spinning,
This
procedure
sample recovery
AG button when
additional
an
HIGH
provided
LOW
or
The
of
the
should
the
mode,
run
a
power
Cellwasher
to allow
following features
the
rotor
will
5
never
only.
seconds
press
continue
failure
sample
be
HOLD
2
used
of
lid
agitation.
to
until
or system
will
recovery
routinely
is
provide
may
not
the
not
in
When
Wait until the
mechanical
before
injury.
The
mechanical override
Depending on
lid
override is located in
object
the side of the
lid
or
the
will
the
main
the
your
side
into the hole and
open.
of the
cabinet, insert
power
override. Reaching
rotor
instrument,
rotor
has
is located
cabinet
the
WARNING
shuts
stopped spinning
push;
off,
stops spinning
the
as shown
lid,
insert
if
straightened
a
on
it is the
the
the left
override
in
brake
into the
could
figure
small
a
will
before
side of the
is
small
paperclip
not
rotor
cause personal
located
2-5. If the
screwdriver
pinhole
operate.
using
the
chamber
Cellwasher
in
either the
mechanical
or
located in
and
push.
similar
The
2
.
2-17
Ccllwasher
MECHANICAL
HOLE
OVERRIDE
FOR
2
Service
HOLE
MECHANICAL
OVERRIDE
FOR-
SORVALL®
Instruments
HOLE
2-6.
The
Condensed
section
with the
in the
the first
LID
IN
Condensed
are
Cellwasher
Cellwasher
(CURRENT LOCATION) PINHOLE IN
Figure
provided
Operating
Operating
2
2-5.
Location
Instructions
Instructions
a reference
as
Refer to
2.
Instruction Manual before
of
Mechanical Override
appended
for
the
complete
time.
the
SIDE (PREVIOUS
at
operator
operating
using
LOCATION)
the
end of
who is
instructions
the
centrifuge for
this
familiar
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Service
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SORVALL®
Instruments
Cellwasher
2
Service
This section Cellwasher customer's followed be performed personnel.
Because
can cause
must not
in this
3-1. Inspection
The following
should processed procedures
instrument.
be
Sections.
describes routine maintenance
2
that should
responsibility to
when
necessary.
a representative
by
tampering
severe
attempt
instrument.
and
Cleaning
preventive maintenance
performed
in the
have been followed before
daily.
Cellwasher
to
be
If
further maintenance
with
electrical
test
If
MAINTENANCE
procedures
performed on a regular basis.
make
WARNING
high
or
etiologic
2,
of
repair
be
sure
DuPont
voltage
shock/
sure
that
other qualified service
or
electrical
untrained
electrical
any
checks and
biohazardous
or
appropriate
inspecting or
these procedures is
required,
cleaning procedures
circuits
personnel
circuits
materials
decontamination
cleaning
for
It is the
it
the
are
should
are
the
Daily Inspection
a.
worn or
If is until it
Check ring assembly
contains accumulated
Inspect any
Inspect
1.
2.
3
.
all
sealing surfaces,
defective
the
screen on
obstructions.
the
stainless steel
Remove
Remove
chamber,
Inspect Replace
inspection reveals
not functioning
the
the
directly beneath
the
if
is
repaired.
tubing,
for cleanliness and
parts.
material.
the
rotor and
rotating
rotating necessary.
that
properly,
Replace
bottom of
rotating
distributor.
bowl
bowl
assembly
NOTE
any
assembly
the
do
part
liners, and
good
tubing
the
bowl
rotor.
for
of the
not
use
condition.
if it is
Cellwasher
assembly
located
sign
any
Cellwasher
the instrument
the
in the
of
collecting
Replace
cracked
2
and
as follows:
corrosion.
2
any
or
remove
rotor
3-1
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