Slee Cryostat MEV Operating Instructions Manual
Instructions – MEV
OPERATING
INSTRUCTIONS
INS2000GB
2011-12-11
Cryostat
MEV
1
Instructions – MEV
2
Instructions – MEV
3
CONTENTS
1.
INTENDED USE
............................................................
4
2.
SYMBOLS
............................................................
4
3.
SAFETY NO
TES
............................................................
4
4.
COMPONENTS
............................................................
8
5.
SPECIFICATIONS
............................................................
9
6.
UNPACKING AND INSTALLA
TION
............................................................
10
7.
INITIAL OPERATION
............................................................
1
3
8.
OPERATION OF MICROTOME
............................................................
1
9
9. OPE
RATION OF CRYOSTAT
............................................................
28
10
.
CLEANING AND
MAINTENANCE
............................................................
3
2
11
.
SERVICE
............................................................
3
4
12
.
OPTIONAL ACCESSORIES
............................................................
3
5
13
.
WARRANTY
............................................................
3
6
14
.
DISPOSAL
............................................................
3
6
Instructions – MEV
4
1. INTENDED USE
The floor standing open top cryostat MEV is intended for rapid freezing and cutting thin
sections of frozen specimens for professional use in routine and research laboratories in
the fields of biology, medicine and industry.
The system of knife advance operates very reliable from 0.5 to 100 µm with trimming
function up to 300 µm. The quality of cutting of this microtome is increased by the
automatic retraction during the upstroke of the specimen, which avoids rubbing on the
disposable blades or microtome knives. This stops rapid deterioration of the disposable
blade or microtome knife.
2. SYMBOLS
Dangers, warnings and cautions are marked by this symbol
i
Special instructions regarding the operation of the
instrument are marked by this symbol
3. SAFETY NOTES
The SLEE Medical open top cryostat MEV is provided with the following safety features:
MEV
Hand wheel stop
••••
Finger protection for Knife- and blade-holder
••••
The institution which owns the unit and the persons working with the unit, servicing or
repairing it have the responsibility for a hazard-free use.
Instructions – MEV
5
3.1 Hand wheel stop
Always use the finger protection
with the knife-/blade holder and put
hand wheel in stop position
• before working with the
knife or specimen
• before changing the
specimen
• during break time
Hand wheel stop released. Movement.
Hand wheel stop activated. No movement.
The
hand wheel
can be stopped in
any position. For this purpose move
the stop lever towards the centre.
For releasing the stop please turn
the lever towards the outside again.
Instructions – MEV
6
3.2 FINGER PROTECTION
Use the finger protection always
• Before you start any work with
the knife or specimen
• Before changing the specimen
• During work break
Finger protection released.
Finger protection activated.
Instructions – MEV
7
3.3 ELECTRICAL POWER CONNECTION
Do not use any extension lead.
Make sure that electric power is constant:
• This should be examined during installation of the unit by a
competent person
• Use a dedicated fuse for the unit
• Do not connect another unit to the same power circuit.
• Before turning on the instrument, check if the voltage of the mains
supply is identical with the name plate of the unit
3.4 WORKING WITH KNIVES AND DISPOSABLE BLADES
Microtome knives and disposable blades have extremely sharp edges and this can lead
to injuries.
Please be extremely careful when handling microtome knives and disposable blades.
Do not place microtome knives or disposable blades at unsecured areas.
Never position microtome knives or dis
posable blades with the sharp edge
pointing upwards.
Store blades in a covered container. Use a container that has guides to
hold the blades rigid.
Never try to catch a falling microtome knife.
Always insert the specimen first and then the microtome kn
ife or
disposable blade.
To avoid compression or knife marks, ensure that the blade is clean.
Instructions – MEV
8
4. COMPONENTS
The SLEE open top cryostat MEV is provided with the following standard components:
MEV
Basic unit with refrigeration system
••••
Semi-automatic rotary microtome
••••
Operation manual
••••
2x Specimen holder
(1x Ø 22mm; 1x Ø 35 mm)
••••
clear-cut
Brush
••••
Anti-roll plate
Ready to use
••••
1x Bottle cryostat low temperature oil
••••
4x 100 ml CryoGlue embedding medium
••••
1 Box of disposable blades (50 pcs.) or 1
steel knife (16 cm C-profile)
••••
Set of Allen keys
••••
Instructions – MEV
9
5. SPECIFICATIONS
Operating temperature range
+10 °C to +
25 °C
Section thickness setting range
0.5 -
100 µm
Section thickness selection
MEV
0 - 2 µm in 0.5-µm steps
2 - 20 µm in 1-µm steps
20 - 50 µm in 2-µm steps
50 – 100 µm in 5 µm steps
Horizontal specimen advance
28 mm
Vertical specimen stroke
57 mm
Specimen retraction on return stroke
0 – 200 µm; selectable
Dimensions and weight
[width x depth x height]
600 mm
x
680 mm x
1130
mm
Weight
without accessories
110 kg
Specimen orientation, horizontal
8°
Specimen orientation, vertical
8°
Specimen orientation,
z-axis
360°
Trimming thickness
0 – 300
µm, freely programmable
Instructions – MEV
10
6. UNPACKING AND INSTALLATION
6.1 UNPACKING THE INSTRUMENT
In standard design you will receive the unit in a wooden box standing on a wooden
pallet. It is equipped with four rollers and two feet, adjustable in height in the front.
Remove the screws from the wooden box with the head screws screwdriver or battery
screwdriver with insert for head screws.
Option 1
If the unit is near the installation site, remove the front cover of the cooling unit (see
section simple service work) and lift the unit slightly with one hand at the outer edge
and the other hand at the rear edge of the cover, move approx. halfway off the pallet
and lower the front to the ground. After this the unit is lifted at the rear, the pallet is
removed and the rear part lowered to the ground.
Option 2
If the unit has to be transported further the wheelbarrow is useful. Push the
wheelbarrow from the rear under the unit and lift it carefully off the pallet.
Extend the two front feet to stabilize the cryostat in its position by turning the
supporting screws counter clockwise.
For repacking use the original cases. Keep the packing material.
Instructions – MEV
11
6.2 INSTALLATION SITE REQUIREMENTS
The site for installation should meet the following requirements:
• A maximum ambient temperature of 25
°C
• No direct sunlight into the coolin
g chamber
• A minimum distance between wall and rear of the instrument of 10
-
20 cm
should be guaranteed
• A free ventilation of fresh air from underneath the instrument should be
guaranteed
6.2 INSTALLATION
For opening the chamber push the window to the top.
Remove the transport security underneath the specimen holder and position the waste
tray.
After installation, wait at least 4 hours before switching the instrument
on. Failure to comply may result in severe damage to the instrument.
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