SKC biosampler 225-9595, biosampler 225-9594, biosampler 225-9595K4, biosampler 225-9597, biosampler 225-9593 Operating Instructions Manual
Operating Instructions
863 Valley View Road, Eighty Four, PA 15330 USA
Tel: 724-941-9701 • www.skcinc.com
BioSampler® Bioaerosol Collection Device
Cat. No. 225 Series
Introduction
Description
Inlet
Inlet section
Outlet to pump
Nozzle section*
3 Nozzles (sonic
orifi ces)
Figure 1. SKC BioSampler Components
BioSampler shown with 20-ml vessel (5-ml vessel available)
* Etched nozzle sections and collection vessels are supplied as a matched set. Use the etched identifi er
to match the two components during sampler reassembly for optimal fi t and performance.
Collection
vessel*
Checking BioSampler/Kit Contents
Use the table below to verify that you received all items associated with the Cat.
No. ordered. If you are missing items, contact SKC at 800-752-8472 (U.S. only) or
724-941-9701.
If You Ordered Cat. No. Your Package Should Contain
225-9595
225-9595K4
225-9593
225-9597
225-9594
3-pc glass 20-ml BioSampler (inlet section, nozzle
section, and collection vessel), does not include ground
joint cap; inlet and nozzle sections are a matched set
Four 3-pc glass 20-ml BioSamplers, does not include
ground joint cap; inlets and nozzle sections are each a
matched set
3-pc glass 5-ml BioSampler (inlet section, nozzle section,
and collection vessel), does not include ground joint cap;
inlet and nozzle sections are a matched set
3-pc glass 20-ml BioSampler, two 20-ml collection
vessels (bottoms) with ground joint caps, 1 BioSampler
case with mounting rod, and 1 ViaTrap (120 ml)
collection liquid
3-pc glass 20-ml BioSampler, two 20-ml collection
vessels (bottoms) with ground joint caps, 1 BioSampler
case with mounting rod, 1 ViaTrap (120 ml), 1 Vac-U-Go
Sample Pump (115 V, sonic fl ow), reinforced tubing for
connecting pump to BioSampler, and 1 rotameter
Required Equipment/Media
• BioSampler
• Sample pump, with vacuum gauge or manometer, capable of maintaining
sonic fl ow, such as Vac-U-Go (Cat. No. 228-9605), supplied separately or in
kits; see Checking BioSampler/Kit Contents and Accessories/Replacement Parts
• Collection liquid or coating (see Choose a Collection Liquid/Coating)
• 3/8-inch ID silicone tubing
®
• 1/4-inch ID Tygon
• Liquid trap
• Pipe e
• Aluminum foil or Parafi lm
Tip
For optimal operation and convenience, use BioSampler with the
BioSampler case and mounting rod accessories (available in BioSampler
Mini Kit Cat. No. 225-9597 and supplied in BioSampler Complete Kit
Cat. No. 225-9594).
tubing
2
Operation
Set/Calibrate Pump Flow
The BioSampler nozzle section contains three tangential 0.630-mm nozzles that
act as sonic orifi ces. Flow through the nozzles remains constant at approximately
12.5 L/min when used with a pump, such as the Vac-U-Go, that maintains a pressure
drop of 0.5 atmosphere (15 in Hg) or more across the sampler at normal atmospheric
conditions (sonic fl ow).
1. Prepare the calibration train. (Figure 2)
a. Connect the fl owmeter outlet to the BioSampler inlet.
Inlet
Outlet
20
15
10
LPM AIR
5
2
Rotameter
Valve adjust
Vacuum gauge
15
20
25
InHg
vac
30
ASHCROFT
Air Sampler
Cat. No. 228-9605
SKC Inc. Eighty Four, PA
Sample Pump
(Vac-U-Go shown)
Inlet
10
5
®
Tubing
Figure 2. Calibration Train
Inlet
Tubing
BioSampler
If using the rotameter supplied in Cat. No. 225-9594, use a length of 3/8-inch ID
silicone tubing to connect the rotameter outlet to the BioSampler inlet.
b. Connect the BioSampler outlet to the sample pump inlet.
2. Use the valve adjust on the pump to achieve sonic fl ow (vacuum gauge or
manometer indicates between 15 and 30 inches Hg vacuum).
3. Observe the fl ow rate indicated on the fl owmeter and record as Q
L/min (to
s
be used to calculate sample volume).
Calibration Note
If pump fl ow rate changes by ± 5% between the baseline calibration (fi rst calibration
when sampler is new) and subsequent calibrations, it may indicate a broken or clogged
nozzle. Replace the nozzle section of the BioSampler and re-calibrate. The new calibrated
fl ow rate (with replacement nozzle section) will likely diff er slightly from previous
calibrations (each BioSampler has a unique fl ow rate that is approximately 12.5 L/min).
Use this new fl ow rate as the new baseline fl ow rate for this sampler.
3
Prepare the BioSampler Collection Vessel
Before sampling, sterilize the BioSampler, choose a collection liquid or coating, and
fi ll or coat the collection vessel as follows.
Sterilize the BioSampler
Sterilize the inlet, nozzle section, collection vessel, and ground joint cap in
an autoclave or oven heated within a range of 320 to 356 F (160 to 180 C) for 180
to 240 minutes before each use. This form of sterilization will kill bacteria, fungi,
and DNA.
To prevent fritted joints from sticking together during heating, disassemble
sampler components (three pieces) before placing them in autoclave or oven.
During reassembly, match the etched identifi er on nozzle section with the
identifi er on collection vessels for optimal fi t and performance.
Choose a Collection Liquid/Coating
BioSampler can be used for short-term and long-term sampling. See the following
table for recommended collection liquids and coatings based on the desired sample
time. Contact the laboratory for specifi c requirements, including sterilization.
Desired Sampling Time Recommended
Liquid/Coating
Shorter than 30 minutes
(with liquid)
Shorter than 30 minutes
(with coating)
Longer than 30 minutes Non-evaporating liquid
Sterile distilled water,
physiological saline,
phosphate buffered saline
(PBS), nutrient broth, or
peptone water
Petroleum jelly/hexane
solution coating
such as ViaTrap
or glycerol
Note: ViaTrap® mineral oil
requires sterilization before
use. See Note in Fill or
Coat the Collection Vessel
mineral oil
Notes/Cautions
Do not use water or waterbased liquids for sampling
periods longer than 30
minutes or for collecting
hydrophobic particles such
as fungal spores. Contact
laboratory for specifi c
requirements.
Do not use this method for
sampling periods longer
than 30 minutes.
Collection effi ciency
remains virtually
constant during 8-hour
sampling period.
Glycerol is not
recommended for
viable microorganism
collection; it can cause
microorganisms to die
from osmotic pressure.
ViaTrap may not
be suitable for PCR
analysis.
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