Operating Instructions
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Tel: 724-941-9701 Fax: 724-941-1369 e-mail: skctech@skcinc.com
863 Valley View Road, Eighty Four, PA 15330 USA
MethChek Immunoassay Wipe Kit for
Methamphetamine Residue on Surfaces
Developed by the U.S. National Institute for Occupational Safety and Health (NIOSH),
Centers for Disease Control (CDC), MethChek
wipe kits identify methamphetamine residue on surfaces at or above relevant
state cleanup levels. Ideal for pre and post-remediation evaluation of clandestine
meth lab sites, MethChek provides on-the-spot answers without waiting for lab
results. MethChek kits are available with a 0.05, 0.1, 0.5, or 1.5 microgram per 100 cm
lower limit of identifi cation (LLOI).
®
semi-quantitative immunoassay
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Using MethChek 100, 500, and 1500 Wipe Kits
(100 ng, 500 ng, and 1500 ng LLOI)
See Cautions and Handling on pages 9-10 before proceeding.
Preparing to Sample
1. Put on clean gloves supplied in kit.
Use new, clean gloves for each test.
2. Place supplied wax paper on a level surface.
3. Place Step 2 Extractor bo le on wax paper.
4. Remove plunger from syringe and place on wax paper.
5. Place supplied template on test surface.
SKC recommends adhering template to surface.
Consult state meth sampling guidelines for
recommended template locations.
Multiple samples in multiple locations within a
structure are recommended. Consult state meth
sampling guidelines.
Place items on wax paper.
Place template.
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6. Remove screw cap from Step 1 We ing Agent bo le.
Insert and tighten spray nozzle. Prime spray bo le in a
waste can.
7. Remove gauze pad from package and spray gauze
2 times with Step 1 We ing Agent at a distance of
approximately 4 inches.
Spray 2 times with Step 1
Wetting Agent.
Sampling
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Using fi rm hand pressure, collect the sample by wiping the surface inside the
template with the moistened gauze pad in the following pa erns:
and and
a. Left to right in a “W”
pattern
b. Left to right in a “Z”
pattern
Do not trap or push surface residue under edges of template.
When wiping, orient the wipe to concentrate the sample in one area on the
wipe. This will enhance extraction.
Extracting the Sample
1. Insert gauze pad containing sample into syringe.
2. Insert syringe plunger and gently push gauze pad
toward base of syringe.
Press enough to loosely compact gauze pad.
3. Remove syringe plunger.
c. Repeat left to right in a
“W” pattern
Insert gauze into syringe.
Use plunger to loosely
compact gauze.
4. Open Step 2 Extractor bo le, hold syringe over
plastic tray, and pour Step 2 Extractor into syringe.
Pour Step 2 Extractor
into syringe.
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5. Holding the syringe tip over the plastic tray, slowly
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insert and depress plunger to compress and extract
the sample from the wipe until liquid no longer
fl ows.
Depress plunger, collect
liquid in tray.
6. Place syringe tip in liquid in plastic tray and slowly
pull plunger halfway to draw sample back into
syringe.
Draw liquid into syringe.
7. Repeat Step 5.
8. Remove Step 3 Cartridge and disposable pipe e from packet and place on
wax paper.
Ensure surface is level.
9. With pipe e, add 3 drops of extract liquid from
plastic tray to the sample well (marked “S”) on Step
3 Cartridge.
Pipette 3 drops of liquid
into “S” well on cartridge.
10. Observe Step 3 Cartridge. Lines will appear
within one minute. Results will develop
completely within 5 minutes. See Reading
MethChek Results on page 7.
Do not disturb cartridge during sample
development.
Results appear in
≤1 minute. Development
completed in 5 minutes.
11. Once test is complete, write ID on cartridge
and on mini bag, insert cartridge into mini
bag, and transport or store.
ID sample cartridge. Transport
or store in mini bag.
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12. Place disposable items in resealable test packet bag,
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seal, and dispose of as hazardous materials. See
Disposing on page 10.
Do not discard Step 1 We ing Agent solution,
spray nozzle, or bo le unless empty or expired.
Bo le contains enough solution for several
tests.
SKC recommends sealing the Step 1 solution bo le
with the provided screw cap for transport and storage.
Thoroughly wash exterior of bo le with soap and water before
sampling at another site to avoid cross contamination.
Using the MethChek 50 Wipe Kit (50 ng LLOI)
Preparing to Sample
See Cautions and Handling on pages 9-10 before proceeding.
1. Put on clean gloves supplied in kit.
Use new, clean gloves for each test.
2. Place wax paper and supplied items on a level
surface.
Place disposable items in
resealable bag.
Place items on wax paper.
3. Place supplied template on test surface.
SKC recommends adhering template to surface.
Consult state meth sampling guidelines for
recommended template locations.
Multiple samples in multiple locations within a
Place template.
structure are recommended. Consult state meth
sampling guidelines.
4. Remove screw cap from Step 1 We ing Agent
bo le. Insert and tighten spray nozzle. Prime
spray bo le in a waste can and then spray co on
swab tip 2 times at a distance of approximately
4 inches. Rotate tip between sprays.
Spray 2 times with Step 1
Wetting Agent.
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