SKC 500-400 User Manual

Operating Instructions

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863 Valley View Road, Eighty Four, PA 15330 USA

Passive Sampler for Amines

Catalog No. 500-400

Developed in collaboration with the National Institute of Working Life in Umea,
Sweden, the SKC UME
range. The UME

x

400 Passive Sampler collects amines in the 0.5 to 10 ppm

x

400 Passive Sampler contains a tape treated with 1-Naphthyl­isothiocyanate (NITC). Each sampler incorporates a “blank/correction” section
in addition to the active sampling section. Analysis is by high-performance
liquid chromatography with UV detection (HPLC-UV) for identifi cation of
amines that may be present in the sample. Designed for single use, the SKC

x

UME

400 Passive Sampler is packaged in an aluminized pouch for easy sample

transport to a laboratory.

Collar clip

NITC-treated
tape (contained
behind diffusion

Sliding cover in
"off" position

Sample blank/
correction
(contained
within housing)

Diffusion
barrier
exposed

barrier)

UMEx Sampler with sliding

cover in "off" position

www.skcinc.com

UMEx Sampler with sliding

cover in "on" position

Performance Profi le

Sampling Rates: Compound Sampling Rate RSD
(ml/min)

Methyl amine 18.4 14.0
Dimethyl amine 18.2 5.8
Isopropyl amine 13.9 9.5
Allyl amine 22.4 7.7
n-Butyl amine 18.1 9.2

Concentration Range: 0.5 to 10 ppm

Detection Principle: Formation of a stable amine derivative, made from a

reaction of the amine with NITC

Analysis Method: Analysis by HPLC with UV detection at 254 nm

Accuracy: ± 25% for all amines except methyl amine

± 35% for methyl amine

Shelf-life: 12 months from date of manufacture in freezer

Storage: Before use: ≤39.2 F (4 C)

After use: Samples can store at ambient temperatures

for 14 days

Do not store with food.

Temperature Effects: No effect on sampling rate between 50 and 86 F

(10 and 30 C)

Humidity Effects: No effect on sampling rate from 10 to 80% relative humid-

Wind Velocity Effects:

Interferences: None found; highly specifi c for amines

Validation: Partial

Dimensions: 3.4 x 1.1 x 0.35 in (8.6 x 2.8 x .89 cm)

Weight: 0.38 oz (10.8 g)

Slide Cover: Blue

For storage greater than 14 days, store at ≤39.2 F (4 C).

ity (RH)

No effect from 5 to 100 cm/sec

Sampling Instructions

Caution s: • Store at ≤ 39.2 F (4 C) before use.

• Do not store with food.

• Before sa mpling, chec k the expirat ion date on the label on the

• SKC recommends using gloves when handling chemically

• UME

outside of the pouch. Do not use a er the last day of the month
indicated.

treated media.

x

samplers are designed for single use. Do NOT reuse

x

UME

samplers.

1. Open the pouch and remove the sampler. Do not discard the pouch; use it

to send sampler to the laboratory. Store pouch away from potential amine

sources.

2. Enter appropriate information on the label.

3. Position the sampler on a worker’s collar for personal sampling or in an ap­propriate location for area sampling.

4. Slide the sampler cover to the “on” position to begin sampling. Enter the
sample start time in the space provided on the back of the sampler.

5. A er sampling for the desired time, up to 8 hours, slide the sampler cover to
the “off ” position to stop sampling. Enter the sample stop time in the space
provided on the back of the sampler.

6. Place the sampler in the original pouch.

7. Seal the pouch. Send to an accredited laboratory for analysis.

Analysis Instructions

Calibration Standards

To prepare standards of the amine derivatives, simply add a known amount
of the amine to a vial containing 3 ml of acetonitrile and a section of the NITC­treated tape.

Sample Preparation

Collar clip

1. Remove the sampler from the pouch and
the sliding cover from the sampler. Use
forceps (cleaned with acetonitrile) to li

Reactive tape (sample)

Diffusion plate

out the reactive tape from each section.
Place each in a sealed vial. This provides a
sample and a blank.

Note: The blank/correction section has an

indentation for easy identifi cation.

Body

2. Add 3.0 ml of acetonitrile to each vial.

3. Perform sample analysis on both tapes.

Sample Analysis

1. The amine derivative is desorbed from

both the sample and control tapes
by placing each in its own glass vial con-

Reactive tape
(blank/correction)

Sliding
cover

taining 3.0 ml of acetonitrile and shaking
them for one minute by hand.

2. A 20-microliter portion of this sample is injected into a liquid chromatograph
and analyzed using a C18 column and a mobile phase consisting of 60%
acetonitrile and 40% water.

3. The amine derivative is detected by UV detection at 254 nanometers (nm).

4. Quantitation is performed using calibration standards prepared as described
in Calibration Standards above.

References

Lindahl, R., Levin, J.O., and Andersson, K., “Determination of Volatile Amines in Air by
Diff usive Sampling, Thiourea Formation, and High-performance Liquid Chromatography,”
Journal of Chromatography, 643, 1993, pp. 35-41

UMEx Passive Samplers*

x

UME

400† for amines, pk/10

x

UME

100† for formaldehyde and other aldehydes, pk/10

x

UME

200 for sulfur dioxide and/or nitrogen dioxide, pk/10

‡

Catalog No.
500-400
500-100
500-200

* Limited shelf-life. Do not store with food.
† Storage at ≤39.2 F (4 C) required

x

‡ UME

samplers are designed for single use only. Do NOT reuse UMEx samplers.

SKC Limited Warranty and Return Policy

SKC products are subject to the SKC Limited Warranty and Return Policy, which
provides SKC’s sole liability and the buyer’s exclusive remedy. To view the com­plete SKC Limited Warranty and Return Policy, go to h p://www.skcinc.com/
warranty.asp.

Form 40068 Rev 1111