Sigma 3-16K Operating Manual

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Refrigerated Centrifuge

3-16K

Operating Manual

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Thank you very much for your confidence in the products of our company. Our centrifuges are manufactured carefully according to the highest quality standards and we

are sure that your demands will always be fulfilled. Please read this manual carefully before installation of the centrifuge to ensure a proper and

safe operation. We are wishing you a successful use of the centrifuge.

SIGMA Laborzentrifugen GmbH P.O. Box 1713 - 37507 Osterode/Germany Phone 05522/5007-0 - Fax 05522/500712 Internet: www.sigma-zentrifugen.de eMail: [email protected] SIGMA Service-Tel. 05522/5007-25

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Konformitätserklärung

(73/23/EWG; 89/336/EWG; 98/37/EWG)

Statement of Conformity

(73/23/CEE; 89/336/CEE; 98/37/CEE)

Déclaration de conformité

(73/23/CEE; 89/336/CEE; 98/37/CEE)

Die nachfolgend bezeichnete Maschine wurde in Übereinstimmung mit den Richtlinien 73/23/EWG; 89/336/EWG und 98/37/EWG hergestellt und geprüft. The following machine is manufactured and tested in compliance with directions 73/23/CEE; 89/336/CEE and 98/37/CEE. La machine désignée ci-dessous est produit et examiné conforme aux directives 73/23/CEE; 89/336/CEE et 98/37/CEE

Bezeichnung der Maschine: Laborzentrifuge Machine: Laboratory Centrifuge Désignation de la machine: Centrifugeuse de laboratoire

Maschinentyp : 3 – 16K Type: Type de la machine:

Bestell Nr. : 10280, 10281, 10282, 10283 Part No.: Réf. usine:

Normen: EN 61010-2-020 Standards: EN 61000-3-2 ; EN 61000-3-3 Normes : EN 61326

Sigma Laborzentrifugen

An der Unteren Söse 50 D-37520 Osterode

19.08.2002 Geschäftsführer Managing Director Directeur Gérant

...........................................................................

Fabr. Nr. Serial No. Numéro de fabrique

Konformitätserklärung dreisprachig 3-16K 20020819.DOC

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General Information

1.1 Technical data

1.2 Suitable accessories

1.3 Scope of supply

1.4 Standards and regulations

1.5 Safety instructions

1.6 Symbol table

Description of the Centrifuge

2.1 General outlay

2.2 Construction and constructive safety measures

2.3 Drive

2.4 Operation and display

2.5 Electronics

2.6 Safety devices

2.6.1 Lid lock, cover closing device

2.6.2 Standstill monitoring

2.6.3 System check

2.6.4 Ground wire check

2.6.5 Imbalance Monitoring System

Installation and Start-up

3.1 Unpacking of the centrifuge

3.1.1 Transport safety device

3.2 Installation

3.2.1 Site

3.2.2 Connection

3.2.3 Fuses / emergency circuit breaker on site

3.3 Installation of rotors and accessories

3.3.1 Fastening of angle rotors with hermetically sealed lid

3.4 Initial start-up

3.4.1 Switching on of the centrifuge

3.4.2 Opening of the lid

3.4.3 Installation of a rotor

Operating Elements

4.1 Operating panel

4.1.1 Start-key / Saving a program

4.1.2 Stop-key with preselection of the soft stop and/or soft start function

4.1.3 Lid-key

4.1.4 Knobs

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4.2 Displays

4.2.1 Speed/RCF (Relative centrifugal force)

4.2.1.1 Speed

4.2.1.2 Relative centrifugual force (RCF)

4.2.2 Time

4.2.3 Standard time mode

4.2.3.1 Continuous operation mode

4.2.3.2 Short-time operation

4.2.3.2.1 Short-run 1

4.2.3.2.2 Short-run 2

4.2.4 Temperature

4.2.5 Preselection of a rotor

Notes for Centrifugation

5.1 Practical notes for centrifugation

5.2 Forbidden centrifuging operations

Care and Maintenance

6.1 Care and cleaning of centrifuge

6.2 Care and cleaning of accessories

6.3 Rotor pins

6.4 Glass breakage

6.5 Care and cleaning of the condenser

6.6 Sterilization and disinfection of rotor chamber and accessories

6.6.1 Autoclaving

6.7 Checks by operator

7. Appendix

7.1 Mathematical relations

7.1.1 Relative Centrifugal Force (RCF)

7.1.2 Density

7.2 Error correction

7.2.1 Centrifuge cannot be started

7.2.2 Centrifuge decelerates during operation

7.2.3 Lid cannot be opened/closed

7.2.4 Emergency lid release

7.2.5 Problems with the centrifuge?

7.3 Error mode

7.3.1 Error codes

7.4 Diagram speed/gravitational field

7.5 Declaration of decontamination/Return declaration

7.6 Leaflet

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1.1 Technical Data

Manufacturer: S I G M A

Laborzentrifugen GmbH

37520 Osterode Type: 3-16K Electr. connection: Protection class: Rated power (kW) Drive: Refrigerating machine: Max. current (A):

Power data: Max. speed (rpm): Max. capacity (ml): Max. gravitational field (x g): Max. kin. energy (Nm): Further parameters Time range:

Temperature setting range: Dimensions: Depth (mm): Width (mm): Height (mm): Weight (kg): EMC (acc. to EN 55011): Noise level (dBA):

Notes of user: Serial number:

Supply date: Inventory number: Location: Responsibility:

The figures are valid for an ambient temperature of +23 °C +/- 2 °C and nominal voltage +/- 5 %. The minimum temperatures during run are < +4 °C a nd depend on type of rotor, speed and ambient temperature. (Allowable ambient temperature +10 °C - +35 °C; max . humidity 80 %.) Subject to technical alterations.

see nameplate

1,01

0,46

0,55

5,5 (230 V/50 Hz) respectively

11 (120 V/60 Hz)

15 300

1000

23 031

9 970

0 - 30 min/continuous run/

short-time operation

-10 to +40 °C

600

630

355

Class B

< 65

.....................................................

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1.2 Accessories Suitable for SIGMA 3-16K

Part No.

11180 Swing-out rotor for 4 buckets for the

13194 Bucket for rectangular carriers 18310,

18310 Rectangular carrier for 4 culture tubes

18311 Rectangular carrier for 10 culture tubes

13180 Rectangular bucket, aluminium, sealable

17112 Sealing cap, polysulfone, clear, incl. 2 clips

18000 Rectangular carrier, undrilled for special

18002 Rectangular carrier for 20 reaction vials

18003 Upper part for 18002 for 20 reaction vials

18005 Rectangular carrier for 20 RIA-tubes 5 ml,

18007 Rectangular carrier for 20 glass tubes 7 ml,

Description

following accessories:

18311 (culture tubes 15 resp. 50 ml), max. radius 16.8 cm, min. radius 6.6 cm

50 ml, e.g. 15151

15 ml, e.g. 15115

with cap 17112, suitable for the system of rectangular carriers, suitable for 11180, max. radius 17.1 cm, min. radius 7.6 cm

17118, suitable for 13180

tubes 85 - 110 mm long, polyallomer

1.5 - 2.2 ml, max. ∅∅∅∅ 11 mm, e.g. 15008, 15040, polypropylene

1.5 - 2.2 ml, e.g. 15008, 15040, can be used together with 18002, polypropylene

max. ∅∅∅∅ 12.2 x 60 - 75 mm, flat and round bottom tubes, e.g. 15060, polyallomer

max. ∅∅∅∅ 12.3 x 80 - 105 mm, flat and round bottom tubes, e.g. 15007, 15027, polyallomer

Max. speed (rpm)

4 500

Max. gravitational field (x g)

3 871/3 894

3 803

3 871

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Part No.

18009 Rectangular carrier for 20 hemolyse tubes,

18010 Rectangular carrier for 12 tubes with screw

18012 Rectangular carrier for 12 Vacutainer-tubes

18015 Rectangular carrier for 12 tubes 10 - 15 ml,

18016 Rectangular carrier for 4 culture tubes

18017 Rectangular carrier for 10 tubes 15 ml, and

18025 Rectangular carrier for 5 glass tubes 25 ml,

18022 Rectangular carrier for 4 sterilin tubes

18030 Rectangular carrier for 5 tubes with screw

18050 Rectangular carrier for 2 tubes 50 ml, max.

Description

max. ∅∅∅∅ 12.8 x 70 - 90 mm, polypropylene

cap 10 - 12 ml, max. ∅∅∅∅ 16.8/17.5 x 60 - 85 mm, e.g. 15000, 15010, 15039, polyallomer

∅∅∅∅ 13.5/18 x 65 - 90 mm, polypropylene

max. ∅∅∅∅ 17 x 90 - 105 mm, e.g. 15015, 15020, 15022, 15023, 15024, polyallomer

15 ml 15115, polypropylene

tubes with stopper, max. Ø 17.2/18 x 80 111 mm, e.g. Monovettes 9 ml and 10 ml, polypropylene

max. ∅∅∅∅ 24 x 85 - 105 mm, e.g. 15025, 15026, polyallomer

30 ml, graduated up to 20 ml, with skirt, incl. cap, max. ∅∅∅∅ 25/31 x 65 - 95 mm, polypropylene, see no. 03008

cap 27 - 30 ml, max. ∅∅∅∅ 25.4/27.5 x 80 - 110 mm, e.g. 15029, 15030, 15032, polypropylene

∅∅∅∅ 35/38 x 85 - 110 mm, e.g. 15049, 15050, 15056, polyallomer

www.bibby-sterilin.co.uk

Max. speed (rpm)

Max. gravitational field (x g)

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Part No.

18051 Rectangular carrier for 2 tubes with screw

18052 Rectangular carrier for 2 culture tubes

18053 Rectangular carrier for 2 culture tubes

18085 Rectangular carrier for 1 tube with screw

18100 Rectangular carrier for 1 tube 100 ml, max.

18105 Rectangular carrier for 20 RIA-tubes 5 ml,

18107 Rectangular carrier for 20 glass tubes 7 ml,

18115 Rectangular carrier for 12 tubes 10 - 15 ml,

18125 Rectangular carrier for 1 bottle with screw

18200 Rectangular carrier for 1 bottle with screw

Description

cap 40 - 50 ml, max. ∅∅∅∅ 29/35 x 80 - 110 mm, e.g. 13055, 15051, 15052, 15054, polypropylene

50 ml 15151, polypropylene

50 ml with skirt, max. ∅∅∅∅ 29.5/38 x 85 - 118 mm, polypropylene

cap 78 - 85 ml, max. ∅∅∅∅ 38/40 x 85 - 112 mm, e.g. 13085, 15074, 15075, 15076, 15080, polyallomer

∅∅∅∅ 45.5/48 x 85 - 110 mm, e.g. 15100, 15102, 15103, 15106, polyallomer

max. ∅∅∅∅ 12.2 x 60 - 75 mm, flat and round bottom tubes, e.g. 15060, decantable, polyallomer

max. ∅∅∅∅ 12.3 x 80 - 105 mm, flat and round bottom tubes, e.g. 15007, 15027, decantable, polyallomer

max. ∅∅∅∅ 17 x 90 - 105 mm, e.g. 15015, 15020, 15022, 15023, 15024, decantable, polyallomer

cap 125 ml, max. ∅∅∅∅ 51 x 90 - 115 mm, e.g. 15125, polypropylene

cap 200 ml, max. ∅∅∅∅ 57 x 90 - 115 mm, e.g. 15202, 15203, polypropylene

Max. speed (rpm)

Max. gravitational field (x g)

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Part No.

11181 Swing-out rotor 48 x 15 ml complete, con-

11182 Swing-out rotor 12 x 50 ml culture tubes

13190 Round bucket, aluminium, sealable with

17190 Sealing cap, polysulfone, clear, suitable for

17350 Round carrier for 24 reaction vials 0.5 -

17351 Round carrier for 12 Monovettes, max.

17352

17353 Round carrier for 16 reaction vials 1.5 -

17354 Round carrier for 16 glass tubes 7 ml, max.

17355 Round carrier for 12 tubes with screw cap

17356 Round carrier for 16 Vacutainer/hemolyse

Description

sisting of rotor 11180, 4 rectangular buckets 13180, 4 carriers 18015 and 48 PStubes 15020, max. radius 17.1 cm, min. radius 7.6 cm

complete, consisting of rotor 11180, 4 round buckets 13190, 4 carriers 17346 and 12 culture-tubes 15151, max. radius

17.2 cm, min. radius 7.5 cm

cap 17190, suitable for the following round carriers, suitable for 11180, max. radius

17.2 cm, min. radius 7.5 cm

13190

0.75 ml, ∅∅∅∅ 8/10 x 28/31 mm, e.g. 15005, polypropylene

∅∅∅∅ 15.5/18 x 50 - 75 mm, polypropylene Round carrier for 25 RIA-tubes, max. ∅∅∅∅ 12.5

x 65 - 80 mm, e.g. 15060, polypropylene

2.2 ml, max. ∅∅∅∅ 11 mm, e.g. ml 15008, 15040, polypropylene

∅∅∅∅ 12.5 x 85 - 115 mm, e.g. 15007, 15027, polypropylene

10 - 12 ml, max. ∅∅∅∅ 16.2/19 x 65 - 90 mm, e.g. 15000, 15010, 15039, polypropylene

tubes/RIA-tubes 5 - 6 ml, max. ∅∅∅∅ 13.5/17.5 x 70 - 90 mm, e.g. 15060, polypropylene

Max. speed (rpm)

4 500

Max. gravitational field (x g)

3 871

3 894

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Part No.

17358 Round carrier for 12 tubes 10 - 15 ml, max.

17345 Round carrier for 5 culture tubes 15 ml

17362 Round carrier for 5 sterilin tubes 30 ml,

17370

17375 Round carrier for 3 tubes 50 ml, max.

17376 Round carrier for 4 tubes with screw cap

17346 Round carrier for 3 culture tubes 50 ml

17385 Round carrier for 1 tube with screw cap

17390 Round carrier incl. rubber cushion 16051

17395 Round carrier for 1 bottle with screw cap

17400 Round carrier for 1 bottle with screw cap

Description

∅∅∅∅ 17.2/19.5 x 90 - 115 mm, e.g. 15015, 15020, 15022, 15023, 15024, and Monovettes 9 ml and 10 ml, polypropylene

15115, polypropylene

graduated up to 20 ml, with skirt, incl. cap, max. ∅∅∅∅ 25/31 x 65 - 95 mm, polypropylene, see

www.bibby-sterilin.co.uk

Round carrier for 5 tubes 25 - 30 ml, max. ∅∅∅∅

25.4/29 x 85 - 115 mm, e.g. 15025, 15026, 15029, 15030, 15032, 15033, polypropylene

∅∅∅∅ 35/38 x 90 - 110 mm, e.g. 15049, 15050, 15056, polypropylene

40 - 50 ml, max. ∅∅∅∅ 29/34 x 85 - 110 mm, e.g. 15051, 15052, 15054, polypropylene

15151, polypropylene

78 - 85 ml, max. ∅∅∅∅ 38/40 x 85 - 115 mm, e.g. 13085, 15074, 15075, 15076, 15080, polypropylene

for 1 tube 100 ml, max. ∅∅∅∅ 45/50 x 85 - 110 mm, e.g. 15100, 15102, 15103, 15106, polypropylene

125 ml, max. ∅∅∅∅ 51 x 90 - 115 mm, e.g. 15125, polypropylene

200 ml, max. ∅∅∅∅ 57 x 90 - 115 mm, e.g. 15202, 15203, polypropylene

, no. 03008

Max. speed (rpm)

Max. gravitational field (x g)

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Part No.

17347 Round carrier for 1 bottle with screw cap

11240 Swing-out rotor for microtiter-plates, incl. 2

11222 Swing-out rotor for microtiter plates, incl. 2

11133 Swing-out rotor for 4 round buckets, for the

13104 Round bucket, aluminium, sealable with

17109 Sealing cap, aluminium, for hermetic

17111 Sealing cap, polysulfone, clear, for

13117 Round bucket, aluminium, for the following

17000 Round carrier, two-piece, undrilled, for

17006

Description

250 ml, max. ∅∅∅∅ 61.5 x 90 - 125 mm, e.g. 15247, 15248, 15249, polypropylene

buckets 13145 and plate holder 17977, radius edge 12.7 cm, radius max. 10.5 cm radius min. 4.65 cm, max. plate height 64 mm

carriers 13222 radius edge 12.3 cm, radius max. 10.5 cm, radius min. 6.5 cm max. plate height 56 mm

following accessories:

caps 17109, 17111, for the following round carriers and the 200 ml bottles 15202 and 15203, suitable for 11133, max. radius

16.1 cm, min. radius 5.7 cm:

sealing of bucket 13104

hermetic sealing of bucket 13104

round carriers and the 200 ml bottles 15202 and 15203, suitable for 11133, max. radius

16.1 cm, min. radius 5.7 cm

tubes 90 - 110 mm long, polyallomer Round carrier for 12 tubes 5 ml, max. ∅∅∅∅

12.5 x 55 - 75 mm, e.g. hemolyse tubes or RIA-tubes 15060, polyallomer

Max. speed (rpm)

3 850

3 000

5 500

Max. gravitational field (x g)

2 105 1 740 770

1 238 1 057 654

5 445

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Part No.

17007 Round carrier for 12 glass tubes 7 ml, max.

17008 Round carrier for 12 reaction vials 1,5 -

17027 Round carrier for 7 Vacutainer-tubes, max.

17012 Round carrier for 6 tubes with screw cap

17013

17015

17018 Round carrier for 4 tubes with sealing cap

17019 Round carrier for 3 culture tubes 15 ml

17025 Round carrier for 2 glass tubes 25 ml, max.

17032 Round carrier for 1 sterilin tube 30 ml,

17030 Round carrier for 2 tubes with screw cap

17049 Round carrier for 1 culture tube 50 ml

Description

∅∅∅∅ 12.3 x 90 - 105 mm, e.g. 15007, 15027, polyallomer

2,2 ml, max. ∅∅∅∅ 11 mm, e.g. 15008, 15040, polypropylene

∅∅∅∅ 13.5/17.5 x 65 - 90 mm, polypropylene

10 - 12 ml, max. ∅∅∅∅ 16.5/17.5 x 58 -90 mm, e.g. 15000, 15010,15039, polyallomer

Round carrier for 9 hemolyse tubes, max. ∅∅∅∅ 12,9 x 85 mm, polypropylene

Round carrier for 7 tubes 10 - 15 ml, max. ∅∅∅∅

16.8 x 90 - 105 mm, e.g. 15015, 15020, 15022, 15023, 15024, polyallomer

10 - 15 ml, max. ∅∅∅∅ 17/20 x 90 - 110 mm and Monovettes, polyallomer

15115, polypropylene, suitable for 13104 without sealing cap

∅∅∅∅ 24.5 x 90 - 105 mm, e.g. 15025, 15026, polyallomer

graduated up to 20 ml, with skirt, incl. cap, max. ∅∅∅∅ 25/31 x 65 - 95 mm, polypropylene, see

www.bibby-sterilin.co.uk

27 - 30 ml, max. ∅∅∅∅ 25.4/27.5 x 85 - 100 mm, e.g. 15029, 15030, 15032, polypropylene

15151, polypropylene

, no. 03008

Max. speed (rpm)

Max. gravitational field (x g)

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Part No.

17050

17052 Round carrier for 1 tube with screw cap

17085 Round carrier for 1 tube with screw cap

17100 Round carrier for 1 tube 100 ml, max.

17101 Round carrier for 1 tube 100 ml, max.

17125 Adapter for 1 bottle with screw cap 125 ml,

11132 Swing-out rotor 28 x 15 ml complete, con-

11224 Swing-out rotor for cytology, complete,

11136 Rotor for reaction vials (max. 60/120 pcs.)

14000 Casset for 20 reaction vials 0.25 - 0.4 ml

Description

Round carrier for 1 tube 50 ml, max. ∅∅∅∅ 35/38 x 85 - 115 mm, e.g. 15049, 15050, 15056, polyallomer

40 - 50 ml, max. ∅∅∅∅ 29/35 x 85 - 115 mm, e.g. 13055, 15051, 15052, 15054, polyallomer

78 - 85 ml, max. ∅∅∅∅ 38/40 x 85 - 112 mm, e.g. 13085, 15074, 15075, 15076, 15080, polyallomer

∅∅∅∅ 45.5 x 85 - 105 mm, e.g 15100, 15103, 15106, polyallomer

∅∅∅∅ 45.5 x 85 - 105 mm, e.g. 15100, 15102, 15103, 15106, polypropylene

max. ∅∅∅∅ 51 x 90 - 120 mm, e.g. 15125, polypropylene

sisting of rotor 11133, 4 round buckets 13117, 4 carriers 17015 and 28 PS-tubes 15020, max. radius 14.9 cm, min. radius

5.7 cm

incl. 4 buckets and carriers for different object carriers. The buckets with object carriers could be locked in 90° position, max. radius 11.3 cm

incl. cover, for carriers 14000, 14002, 14020 and 14021, max. radius 7 cm, min. radius 3 cm. Attention: The vessels could be damaged at speeds exceeding 13 000 rpm

15014, suitable for 11136, polyallomer

Max. speed (rpm)

5 500

3 000

14 500

Max. gravitational field (x g)

5 039

1 137

16 454

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Part No.

14002 Casset for 10 reaction vials 1.5 - 2.2 ml

14020 Casset for 10 PCR tubes 0.2 - 0.5 ml,

14021 Casset for 32 PCR tubes 0.2 ml or 4 strips

11135 Swing-out rotor 24 x 1.5 - 2.2 ml for reaction

12154 Angle rotor 24 x 1.5 - 2.2 ml for reaction

12131 Angle rotor 30 x 1.5 - 2.2 ml for reaction

12348 Angle rotor 48 x 1.5 - 2.2 ml for reaction

12115 Angle rotor for 12 strips with 8 PCR tubes

12111 Angle rotor 10 x 10 - 12 ml for sealed tubes

12157 Angle rotor 20 x 10 - 12 ml for sealed tubes

Description

15008, 15040, suitable for 11136, polyallomer

suitable for 11136, polypropylene

with 8 PCR tubes 0.2 ml each, suitable for 11136, polypropylene

vials e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius 7.4 cm, min. radius 3.5 cm

vials e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius 8.2 cm, min. radius 5 cm, angle 45°

vials e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius10 cm, min. radius 6.7 cm, angle 45°

vials e.g. 15008, 15040, incl. hermetic aluminium lid, max. radius 8.4 cm, min. radius 5.1 cm

0.2 ml each, incl. hermetic aluminium lid, max. radius 9.8 cm, min. radius 7.2 cm, angle 45°

15000, 15010, 15039, incl. hermetic aluminium lid, max. radius 7.6 cm, min. radius 2.8 cm, angle 35°

15000, 15010, 15039, incl. hermetic aluminium lid, max. radius 9.8 cm, min. radius 5.9 cm, angle 25°

Max. speed (rpm)

14 000

15 300

14 000

15 300

14 000

15 300

10 000

Max. gravitational field (x g)

16 215

21 460

21 913

21 984

15 777/21 475

19 890

10 956

Operating Manual SIGMA 3-16K, page 19 of 69

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Part No.

12112 Angle rotor 12 x 15 ml for Greiner-tubes

19777 Angle rotor for 10 culture tubes 15 ml

12158 Angle rotor 6 x 27 - 30 ml for sealed tubes

12150 Angle rotor 6 x 40 - 50 ml for sealed tubes

12156 Angle rotor 8 x 50 ml for sealed tubes 13055

19776 Angle rotor for 6 culture tubes 50 ml 15151,

13060 Adapter for 1 culture tube 15 ml 15115,

13079 Bottom adapter for 1 tube 40 - 42 ml 15051,

12155 Angle rotor 4 x 78 - 85 ml for sealed tubes

12159 Angle rotor 6 x 78 - 85 ml for sealed tubes

Description

with cap 15065 (Greiner no. 187261), max. Ø 17/22 x 95/100 mm, max. radius 10 cm, min. radius 4.8 cm

15115, incl. hermetic aluminium lid, max. radius 9.3 cm, min. radius 3.7 cm, angle 25°

15029, 15030, 15032, incl. hermetic aluminium lid, max. radius 7.9 cm, min. radius 2.3 cm, angle 30°

13055 plus 17054, 15051, 15052, 15054, incl. hermetic aluminium lid, max. radius 8.4 cm, min. radius 2.1 cm, angle 25°

plus 17054, 15051, 15052, 15054, incl. hermetic aluminium lid, max. radius 9.6 cm, min. radius 3.3 cm, angle 25°

incl. hermetic aluminium lid, max. radius

9.3 cm, min. radius 3.1 cm, angle 25°

suitable for 19776, polypropylene

15052, 15054, suitable for 19776, polypropylene

13085 plus 17185, 15074, 15075, 15076, 15080, incl. hermetic aluminium lid, max. radius 9.1 cm, min. radius 1.4 cm, angle 30°

13085 plus 17185, 15074, 15075, 15076, 15080, incl. hermetic aluminium lid, max. radius 9.8 cm, min. radius 2.8 cm, angle 25°

Max. speed (rpm)

9 000

9 500

15 300

11 000

10 000

9 500

10 000

8 000

Max. gravitational field (x g)

9 056

9 384

20 675

11 363

10 733

9 384

10 174

7 012

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Part No.

13080 Adapter for 1 culture tube 50 ml 15151,

13081 Adapter for 1 culture tube 15 ml 15115,

13082

13083

13084

Adapters, tubes and steel tubes

13000 Adapter , POM, for reaction vials 0.25 -

13002 Adapter, POM, for reaction vials 0.5 -

13021 Adapter for PCR tube 0.2 ml, Ø 5.85/6.95 x

15005

15008 Reaction vials 1.5 ml, 1 pack contains 100

Description

suitable for 12155 without lid, 12159, polypropylene

Adapter for 1 tube 40 - 50 ml, max. ∅∅∅∅ 28.8 x 105 - 110 mm, e.g. 13055, 15051, 15052, 15054, suitable for 12155, 12159, polypropylene

Adapter for 1 tube 27 - 30 ml, max. ∅∅∅∅ 25.5 x 90 - 100 mm, e.g. 15029, 15030, 15032, suitable for 12155, 12159

Adapter for 2 tubes 10 - 12 ml, max. ∅∅∅∅ 16/17.5 x 75 - 90 mm, e.g. 15000, 15010, 15039, suitable for 12155, 12159, polypropylene

0.4 ml 15014, suitable for 12110, 12131, 12154, 12348, 14002, 17008, 18002

0.75 ml 15005, ∅∅∅∅ 7.9/10 x 28/31 mm suitable for 12110, 12131, 12154, 12348, 14002, 17008, 18002,

20/23.4 mm, suitable for 12110, 12131, 12154, 12348, 14002, 17008, 18002

Reaction vials 0.5 ml, ∅∅∅∅ 7.9/10 x 28/31 mm, 1 pack contains 100 pcs., suitable for 13002, 17350

pcs., suitable for 12110, 12131, 12154, 12348, 14002, 17008, 17353, 18002

Operating Manual SIGMA 3-16K, page 21 of 69

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Part No.

15040 Reaction vials 2.2 ml, 1 pack contains 100

15014 Reaction vials 0.4 ml (Beckman system),

15060 Polystyrene tube 5 ml (RIA-tube),

13026

17126 Stainless steel sealing cap for 13026 15000

15010 ditto, polycarbonate 15039 ditto, polypropylene 15020

15021 Polypropylene stopper for 15020, 15023 15023

15065

15115 Culture tube with screw cap 15 ml, pointed

15029

Description

pcs., suitable for 12110, 12131, 12154, 12348, 14002, 17008, 17353, 18002

polypropylene, 1 pack contains 100 pcs., suitable for 13000, 14000

∅∅∅∅ 12 x 75 mm, suitable for 17006, 17356, 18005, 18009

Stainless steel tube 10 ml, ∅∅∅∅ 15.7 x 76 mm, sealable with cap 17126, suitable for 12111, 12157

Teflon tube with screw cap 12 ml, ∅∅∅∅ 16.1 x

81.1 mm, suitable for 12111, 12157, 13084, 17012, 17355, 18010

Polystyrene tube 15 ml, ∅∅∅∅ 17 x 100 mm, suitable for 17015, 17358, 18015, 18115

Polypropylene tube 15 ml, ∅∅∅∅ 17 x 100 mm, suitable for 17015, 17358, 18015, 18115

Plastic tube with cap 15 ml, ∅∅∅∅ 17/22 x 94/95 mm (Greiner no. 187261), suitable for 12112

bottom, polypropylene, suitable for 13060, 13081, 17019, 17345, 18016, 19777

Teflon tube with screw cap 28 ml, ∅∅∅∅ 25.3 x 96 mm, suitable for 12158, 13083, 17030, 17370, 18030

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Part No.

15030 Polycarbonate tube with screw cap 30 ml,

15032 Polypropylene tube with screw cap 27 ml,

15049 Polycarbonate tube 50 ml, graduated,

13055 Stainless steel tube 50 ml, sealable with

17054 Stainless steel sealing cap for 13055 15051

15052 Polypropylene tube with screw cap 42 ml,

15054 Polycarbonate tube with screw cap 40 ml,

15048 Polyallomer tube with screw cap 42 ml,

15151 Culture tube with screw cap 50 ml, pointed

13085 Stainless steel tube 85 ml, sealable with

17185 Stainless steel sealing cap for 13085

Description

∅∅∅∅ 25.3 x 98 mm, suitable for 12158, 13083, 17030, 17370, 18030

∅∅∅∅ 25.3 x 97 mm, suitable for 12158, 13083, 17030, 17370, 18030

Ø 34 x 100 ml, suitable for 17050, 17375, 18050

sealing cap 17054, ∅∅∅∅ 29 x 101.5 mm, suitable for 12150, 12156, 13082, 17052, 17375, 18051

Teflon tube with screw cap 42 ml, ∅∅∅∅ 28.5 x 107 mm, suitable for 12150, 12156, 13082, 17052, 17376, 18051

∅∅∅∅ 28.8 x 107 mm, suitable for 12150, 12156, 13082, 17052, 17376, 18051

∅∅∅∅ 28.5 x 107 mm, suitable for 12150, 12156, 13082, 17052, 17376, 18051

bottom, polypropylene, suitable for 13080, 17049, 17346, 18052, 19776

sealing cap 17185, ∅∅∅∅ 38/40 x 100/107 mm, suitable for 12155, 12159, 17085, 17385, 18085

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Part No.

15074 Polycarbonate tube 82 ml with special

15075 Polycarbonate tube with screw cap 82 ml,

15076 Polypropylene tube with screw cap 78 ml,

15080

15102

15103

15125 Polypropylene bottle with screw cap

15202 Polypropylene bottle with screw cap

15203 Polycarbonate bottle with screw cap

13255 Stainless steel bottle 250 ml, sealable with

17256 Stainless steel sealing cap for 13255 15247 Teflon bottle with screw cap 250 ml,

Description

screw cap made of aluminium with seal for high speeds, ∅∅∅∅ 38 x 109 mm, suitable for 12155, 12159, 17085, 17385, 18085

∅∅∅∅ 38 x 112 mm, suitable for 12155, 12159, 17085, 17385, 18085

Polyflor tube with screw cap 81 ml, ∅∅∅∅ 38 x 112 mm, suitable for 12155, 12159, 17085, 17385, 18085

Polypropylene tube 100 ml, ∅∅∅∅ 45 x 100 mm, suitable for 17101, 17390, 18100

Polycarbonate tube 100 ml, ∅∅∅∅ 45 x 100 mm, graduated, suitable for 17100, 17101, 17390, 18100

125 ml, ∅∅∅∅ 51 x 99 mm, suitable for 17125, 17395, 18125

185 ml, ∅∅∅∅ 56,5 x 113 mm, suitable for 13104, 13117, 17400, 18200

200 ml, ∅∅∅∅ 56 x 112 mm, suitable for 13104, 13117, 17400, 18200

sealing cap 17256, Ø 61.4 x 125 mm, suitable for 17347

Ø 61.4 x 122 mm, suitable for 17347

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Part No.

15248 Polycarbonate bottle with screw cap 250

15249 ditto, polypropylene

Glass tubes

15007

15027 ditto, graduated 15015

15024 ditto, graduated 15022

15025

15026 ditto, graduated 15033

15050

15056 ditto, graduated 15100

15106 ditto, graduated

Description

ml, Ø 61.4 x 125 mm, suitable for 17347

Glass tube 7 ml, ∅∅∅∅ 12 x 100 mm, suitable for 17007, 17354, 18007, 18107

Glass tube 10 - 12 ml, ∅∅∅∅ 16 x 100 mm, suitable for 17015, 17358, 18015, 18115

Special glass tube 15 ml, ∅∅∅∅ 17 x 110 mm, suitable for 12150, 12156 with 16018 and for 12155, 12159 with 16019, max. permitted speed 7 000 rpm

Glass tube 25 ml, ∅∅∅∅ 24 x 100 mm, suitable for 17025, 17365, 18025

Special glass tube 30 ml, ∅∅∅∅ 24 x 105 mm, suitable for 12150, 12156 with 16030 and for 12155, 12159 with 16031, max. permitted speed 7 000 rpm

Glass tube 50 ml, ∅∅∅∅ 34 x 100 ml, suitable for 17050, 17375, 18050

Glass tube 100 ml, ∅∅∅∅ 44 x 100 mm, suitable for 17100, 17101, 17390, 18100

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Part No.

Further accessories

16018 Rubber adapter for 15 ml glass tube 15022,

16019 Rubber adapter for 15 ml glass tube 15022,

16030 Rubber adapter for 30 ml glass tube 15033,

16031 Rubber adapter for 30 ml glass tube 15033,

16906 Rubber plate for 18025 16907 ditto, for 18050 16908 16909 Rubber plate for rectangular carriers 18005,

16910 Rubber plate for round carriers 17006,

16911 Rubber plate for round carriers 17050,

17112 Rectangular sealing cap, polysulfone, clear,

17111 Round sealing cap, polysulfone, clear, for

16251 Sealing ring 58 x 3 mm for 13104 17190 Round sealing cap, polysulfone, clear, for

17919 Table for centrifuge made of sheet-steel,

17913 Fasteners for table 17919 Further accessories are available on request.

Description

suitable for 12150, 12156

suitable for 12155, 12159

suitable for 12150, 12156

suitable for 12155, 12159

ditto, for 18100

18007, 18015

17007, 17012, 17015, 17025

17100

incl. 2 clips 17118, for 13180

13104

13190

varnished, with 2 doors and space for accessories, movable on lockable castors, dimensions: w 490, d 635, h 490 mm

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Maximum speed for tubes

Some tubes, e.g. glass tubes, microtubes, culture tubes, Teflon tubes and especially high volume tubes can be used in our rotors, buckets and adapters at speeds exceeding their breaking limit. We recommend to always fill up the tubes and to follow the recommendations of the manufacturer.

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1.3 Scope of Supply

The following belongs to the centrifuge: Connection cable Part No. 269 010

Rotor wrench Part No. 930 100 20 ml slushing oil Part No. 70104 1 tube of grease for load-bearing bolts Part No. 70284

Documentation: Operating Manual

"Rotor and Accessories, Operation and Use" EU-Statement of Conformity Equipment Decontamination Certificate

Accessories according to your order, our order confirmation and our delivery note.

Rotor Part No. Rotor No.

....................... .....................

1.4 Standards and Regulations

Please refer to the enclosed EU-Statement of Conformity.

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1.5 Safety Instructions

According to the German trade association regulation BGR500 chapter 2.11 part 3 the owner of the instrument is advised to take care of the following points:

1. According to BGR500 the owner has to provide operating instructions based on those of the manufacturer and to inform the employees accordingly.

2. For safety reasons these operating instructions must clearly state that the stamped max. speed of the used rotor and the max. allowable filling quantity must not be exceeded.

3. If the density of the material exceeds 1.2 g/cm3, the max. speed of the centrifuge must be reduced (see formula chapter 7.1.2).

4. Operation of the centrifuge in hazardous locations is not allowed.

5. During operation the centrifuge must not be moved. Leaning against or resting on the centrifuge is not allowed.

6. Do not spin explosive or highly inflammable materials.

7. Substances which could damage the material of the centrifuge, the rotors or the buckets anyhow must not be centrifuged or only under consideration of special safety measures. Infectious, toxic, pathogene or radioactive substances must be centrifuged in certified rotors only.

8. Keep a clearance of at least 30 cm around the centrifuge. Dangerous materials of any kind must not be put down or stored in that area.

9. Attention! Defective lid relieving devices could cause the centrifuge lid to fall down (contact Service). Risk of bruising!

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1.6 Symbol Table

International symbols used for the centrifuge: Symbol Title

Gefährliche elektrische Spannung

Achtung, Bedienungsanleitung beachten

Ein (Netzverbindung)

Aus (Netzverbindung)

Schutzleiteranschluß

Erde

Netzstecker ziehen

Vorsicht Quetschgefahr

Drehrichtungspfeil

→

Heiße Oberfläche

Dangerous voltage Courant haute tension

Attention, consult accompanying documents Attention, consulter les documents joints

On (Power) Marche (mise sous tension)

Off (Power) Arrêt (mise hors tension)

Protective earth (ground) Liaison à la terre

Earth (ground) Terre

Unplug mains plug Tirer la fiche de prise

Caution! Risk of bruising Attention! Danger de blessure

Arrow direction of rotation Flèche sens de rotation

Hot surface Surface chaude

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2.1 General Outlay

The new generation of SIGMA laboratory centrifuges is equipped with newest state-of-the-art electronics and is driven by brushless, silent and long-life asynchronous motors.

The problem of carbon brush change is no longer existent and as there is no carbon dust pollution, operation in clean rooms is possible if the appropriate accessories are used.

2.2 Construction and Constructive Safety Measures

The centrifuge is built into a solid steel housing. The centrifuge lid is also made of solid steel. From the back, the lid is secured by solid hinges and at the front by a cover lock.

2.3 Drive

The drive motor is a well dimensioned asynchronous motor.

2.4 Operation and Display

The grafical LCD display is hermetically sealed, operation is executed via two knobs. Any operating status is indicated.

2.5 Electronics

The electronics controlled by a microprocessor allows extensive adaptations of the centrifuge to the different tasks. The following parameters can be set:

− Speed in steps of 1 respectively 100 rpm *

− RCF in steps of 1 respectively 10 x g *

− Time in steps of 1 min respectively 1 sec

− Continuous operation

− Short-time operation

− Temperature setting range between – 10 °C and + 40 °C in steps of 1 °C

− Preselection of rotor

* Preselection of 1 x g respectively 1 rpm by pressing and holding (2 – 3 seconds) the stop key.

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2.6 Safety Devices

Apart from the passive safety devices due to the instrument's mechanical design there are the following active precautions for your safety:

2.6.1 Lid Lock, Cover Closing Device

The centrifuge can only be started when the lid is correctly closed and the lock has latched. The lid can only be opened when the rotor has stopped. If the lid is opened via the emergency release during operation (which is not allowed), the centrifuge will immediately switch off and decelerate brakeless. If the lid is open, the drive is completely separated from the mains supply, that means starting of the centrifuge is impossible (refer to point 7.2.4 "Emergency lid release").

2.6.2 Standstill Monitoring

Opening of the centrifuge lid may only be possible, if the rotor is at standstill. This standstill is checked by the microprocessor.

2.6.3 System Check

An internal system check monitors data transmission and the sensor signals with regard to plausibility. In case of a malfunction an error message is displayed under a number in the “Speed“ and “rcf“ display.

2.6.4 Ground Wire Check

For ground wire check there is a ground screw at the rear panel of the centrifuge. A ground wire check can be carried out using an appropriate measuring instrument.

2.6.5 Imbalance Monitoring System

The display “Imbalance“ indicates by lightning up that the centrifuge is in the still permitted imbalance area. In the event that bigger uneven loading leads to imbalance, the drive is switched off during acceleration or during run. “Imbalance“ is flashing and “ERROR“ is displayed. The centrifuge has to be opened with the lid key. In both cases the loading has to be checked and balanced.

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3.1 Unpacking of the Centrifuge

Open cardboard. Take out the box containing accessories. Remove upper foam cushions. Lift centrifuge upwards with a lifting device or with several persons. When lifting or carrying the centrifuge please always reach under the instrument from the side.

Proceeding when using a slip box: Remove cover of cardboard box and take out accessories. Remove upper foam cushions and remove slip box. Lift centrifuge upwards with a lifting device or with several persons. When lifting or carrying the centrifuge please always reach under the instrument from the side.

Attention: The instrument is heavy!

Please keep case for possible transport of centrifuge later.

3.1.1 Transport Safety Device (made of foamed material)

The SIGMA 3-16K has a transport safety device between the rotor chamber and the motor.

It is accessible after opening of the centrifuge lid (refer to chapter 3.3 up to 3.4.2 or 7.2.4“ Emergency lid release“).

The transport safety device has to be removed, the motor is slightly lifted at the shaft.

Align centrifuge and continue start-up. The transport safety device should be kept for possible transport of the centrifuge

(service, repair).

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3.2 Installation

3.2.1 Site

All energy consumed by the centrifuge is converted into heat and emitted into the ambient air. Therefore, sufficient ventilation is important. As the air-ducts in the unit must be open, keep a clearance of at least 30 cm around the centrifuge. Also, the centrifuge shouldn`t be positioned near radiators and should not be directly exposed to sunshine.

The table should have a solid, even top. For normal operation the ambient temperature should not fall below 10 °C and

not exceed 35 °C. The max. humidity of air is 80 %. During transport from cold to warmer places water will condensate inside the centrifuge. It is important that there is enough time for drying before the centrifuge can be started again.

3.2.2 Connection

The operating voltage on the name plate must correspond to the local supply voltage!

SIGMA laboratory centrifuges are units of safety class I, DIN VDE 0700, and include a three wire power cord 2,5 m long with shockproof right angle plug. The instrument has thermal fuses. In case of a disconnection through the thermal fuses, allow a cool-down phase of two minutes, after which they could be reactivated by a switch.

At the back, next to the mains supply, there is an additional ground wire connection where a separate ground wire can firmly be connected to ground any non-hazardous leakage current. The leakage current is harmless but secondary effects would occur.

3.2.3 Fuses / Emergency Circuit Breaker on Site

Each centrifuge must be protected with a 16 A time lag fuse. An emergency circuit breaker to cut the power to the centrifuge in the event of a

malfunction is required on site. This switch should be located away from the centrifuge, preferably outside the room where the centrifuge is used or at the exit of this room.

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3.3 Installation of Rotors and Accessories

1. Open centrifuge lid by pressing Lid-key.

2. Unscrew rotor tie-down screw from motor shaft (anticlockwise).

3. Lower the rotor straight down onto the motor shaft.

4. Tighten the tie-down screw (clockwise) with the rotor wrench so that the disc

spring is pressed together.

Fastening torque: 1-6/1-13/1-15/2-5/1-15K/2-16/2-16K/

2-16KC: 3-16/3-16K/3-18K/3K 30: approx. 7,5 Nm 4-15/4K15/6-15/6K15: approx. 10 Nm

In the event of frequent use the tie-down screw must be loosened by some turns and fastened again. This should be done once a day or after approx. 20 cycles. This ensures a proper connection between rotor and shaft (please refer to chapter 6.2 "Care and cleaning of accessories" as well).

5. Fill all positions of swing-out rotors with buckets. Make sure that all buckets are inserted correctly.

6. Use only appropriate vessels for the rotor (please refer to chapter 1.2 "Suitable accessories" as well).

7. Fill vessels external to the centrifuge.

8. Put or screw on covers of vessels.

9. Opposite places of the rotors must always be loaded with same accessories and same filling.

10. In angle rotors the plastic vessels must always be totally filled to avoid cracks of vessels and leakages or loosening of the caps in case of partial filling.

Attention, follow the special comments of chapter 1.5.

11. Attention: The centrifuge will absorb smaller differences in weight when loading the rotors. But it is recommended to balance the vessels as accurately as possible in order to ensure a run with minimal vibrations. Should the centrifuge be operated with very uneven load, the imbalance monitoring will switch off the drive. An imbalance warning would be displayed (refer to 4.1).

allowed not allowed

Universal grease

approx. 5 Nm

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12. Rotors with lid should always be run with their lid. The rotor lid is tightened by hand. Correct fastening must be ensured. Attention: The lid screw serves

for fastening of the lid onto the rotor only, not for fastening of the rotor onto the drive! Before installation of the lid, the correct fastening of the rotor

fixing screw must always be checked using a wrench.

3.3.1 Fastening of Angle Rotors with Hermetically Sealed Lid

(Please refer to drawing on next page.)

1. Screw rotor lid (2) onto rotor and tighten it.

2. Lower rotor with lid (2) onto motor shaft.

3. Put rotor tie-down screw (1) onto motor shaft and tighten using the wrench.

4. The rotor can be run without lid (2) as well.

5. The rotor and lid seals (3) must be greased after cleaning.

6. Special instructions for the use of hermetically sealed rotors: All rotors can be installed or removed with closed lid after loosening the rotor

tie-down screw. All rotors are autoclavable (refer to chapter 6.6 "Sterilization and disinfection of rotor chamber and accessories"). To increase life of rotors and seals the rotors must be cleaned with slushing oil and the seals and thread areas with vaseline or grease after cleaning.

Attention! Please follow the special comments of chapter 1.5.

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3.4 Initial Start-Up

Attention! Before initial start-up please take care that your centrifuge is orderly installed

(refer to chapter 3.2 "Installation").

3.4.1 Switching on of the Centrifuge

Press mains switch (on the right side panel).

− The centrifuge display is illuminated.

3.4.2 Opening of the Lid

Press the lid-key.

− The lid opens.

3.4.3 Installation of a Rotor

Put a rotor onto the shaft and fasten it by screwing the rotor tie-down screw clockwise onto the drive shaft. Please use the supplied rotor wrench (refer to chapter 3.3 "Installation of rotors and accessories").

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4.1 Operating Panel

The centrifuge is operated via the operating panel. Keys can be pressed when their LED is on.

When power is applied, all LEDs and the display are illuminated for a short time.

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4.1.1 Start-key

This key can be used for the following:

• starting centrifuge operation when the lid is closed and the start-key is illuminated,

• terminating a previously started deceleration process and restarting the

centrifuge,

• shifting to short-run (refer to chapters 4.2.3.2.1, 4.2.3.2.2),

• saving a program. The parameters speed, time and rotor preselection can be

locked, thereafter a change is impossible. Starting and stopping the centrifuge and opening of the lid is always possible.

Open centrifuge lid. Press the start-key 3 x and hold for approx. 2 seconds when pressing for the third time. The symbol " " in the display is flashing. Cancellation of this function occurs in the same way.

4.1.2 Stop-key with Preselection of the Soft Stop and/or Soft Start Function

This key can be used for the following:

• early terminating the run,

The centrifuge decelerates to a complete stop. Deceleration can be terminated by pressing the start-key again and the centrifuge can be restarted.

• activating the soft stop and/or soft start function resulting in a longer

deceleration and/or acceleration time. When activated “soft stop“ and/or “soft start“ is displayed. This is possible when

- the centrifuge is at standstill: Activation or deactivation of “soft stop“ and/or “soft start“ by pressing

the stop-key.

- the centrifuge is running, stop-key illuminated: Press the stop-key. “soft stop“ and/or “soft start“ can be activated or deactivated during deceleration by pressing the stop-key again.

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4.1.3 Lid-key

This key is used to open the lid. This is possible when

• the centrifuge has come to a complete stop,

• the lid-key is illuminated.

When the lid-key is flashing, the lid has to be opened again. When closing the lid both locks must catch.

4.1.4 Knobs

The parameters are set by turning the knobs.

Left knob:

Activation of “set“ by selecting speed, rcf, time, temperature, rotor or lock.

Remark: “set“ is automatically deactivated after 60 seconds, if no further changes are made, the lock symbol appears.

Thereafter, changes are impossible.

Right knob:

Preselection of the desired set value after activation of “set“.

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4.2 Displays

(Display completely active) The display consists of the following areas:

- Time

- Symbol lock

- Temperature

- Speed

- RCF

- Rotor, Imbalance, run mode

4.2.1 Speed/RCF (Relative Centrifugal Force)

Rotor dimensions and speed determine the RCF value. Entry of one value automatically determines the other one.

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4.2.1.1 Speed

The display “Speed“ shows the rotations per minute. This value can be set by turning the right knob. Preselection of 1 rpm by pressing and holding (2 – 3 seconds) the stop key.

The actual speed is displayed below the set value.

Activate “set“ in the “Speed“ area by turning the left knob. The speed value can be changed during the run by turning the right knob.

4.2.1.2 Relative Centrifugal Force (RCF)

The relative centrifugal force (RCF) is the acceleration which the sample is exposed to.

The display “rcf“ shows the rcf-value. Activate “set“ in the “rcf“ area with the left knob. The rcf value can be changed by turning the right knob. This is also possible when the centrifuge is running. Preselection of 1 x g by pressing and holding (2 – 3 seconds) the stop key.

Please refer to chapter 1.2 “Suitable accessories“ for the maximum values for the used rotor combination.

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4.2.2 Time

This display shows the total set time, the remaining run time or the elapsed time. The time value as well as different time modes can be set by turning the right

knob.

If the time is changed during centrifugation, the total newly entered period is run, the time elapsed before is not considered.

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4.2.3 Standard Time Mode

For the standard time mode the time is set in minutes. Active “set“ in the time area with the left knob. Enter the time by turning the right knob (the display shows “min“).

This total run time (max. 30 minutes) is displayed by a sequence of bars before starting the centrifuge.

During centrifugation this time is counted down, the sequence of bars indicates the remaining run time. The set time is indicated by one single bar.

The centrifuge run can be early terminated by pressing the stop-key. The set time and the remaining run time remain on the display.

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4.2.3.1 Continuous Operation Mode

During continuous run (∞) the centrifuge accelerates up to the set speed and the run has to be terminated manually.

Activate “set“ in the time area using the left knob. Select ∞ by turning the right knob and press the start-key to activate the continuous run.

Unlike the standard run mode the minutes are counted up. A sequence of bars indicates the elapsed run time. One single bar at ∞ is indicating the continuous run mode.

After 30 minutes the remaining run time is no longer displayed but the run continues.

The continuous run is terminated by pressing the stop-key and the centrifuge decelerates to a complete stop. The elapsed time is displayed in minutes.

The continuous run mode can be left by selecting a set value as well.

4.2.3.2 Short-Time Operation

There are two options:

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4.2.3.2.1 Short-Run 1

Press the start-key during the whole short-run. The centrifuge accelerates with maximum power to the maximum speed. After release of the start-key the centrifuge decelerates with maximum power to standstill.

During short-run the time is counted up in seconds, "sec" is displayed. A sequence of bars is indicating the elapsed run time. One single bar at "short

run" indicates that short-time operation had been selected.

After termination of the short-run the elapsed run time in seconds remains on the display.

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4.2.3.2.2 Short-Run 2

During this short-run the centrifuge is running max. 30 seconds and accelerates to the set speed (rotor dependent).

Select "short run“ by turning the left knob and press the start-key.

During the short-run the time is counted up in seconds. A sequence of bars indicates the elapsed run time. One single bar at "short run“ indicates that shorttime operation had been selected.

After 30 seconds the centrifuge decelerates to a complete stop. The short-run can be early terminated by pressing the stop-key. The centrifuge

decelerates to standstill. The elapsed run time in seconds remains on the display.

The "short run“ can be left by selecting a set value as well.

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4.2.4 Temperature

The centrifuge is cooled with a cooling system. When a run is started, there is a temperature difference between the sample and the rotor which is minimized after a certain operating period has passed.

The scale of the upper display area shows the actual temperature in the chamber. Activate “set“ in the temperature area using the left knob and select the set value by turning the right knob. This is possible in steps of 1 °C.

Temperatures between – 10 °C and + 40 °C can be pre selected. If the set temperature is lower than the actual temperature, the set value is indicated by a flashing bar.

As soon as the lid is opened, the cooling system is disconnected in order to avoid icing of the rotor chamber.

Precooling of the rotor below 0 °C during standstill is not recommended. Differences between actual and displayed temperature and icing of the compressor would be the result. This would reduce life of the compressor. We recommend precooling at 5000 rpm.

The obtainable temperatures depend on rotor type, speed and ambient temperature. (A change of the limit values might cause deviations.)

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4.2.4 Preselection of a Rotor

The display “Rotor“ shows the preselected respectively the recognized rotor. Activate “set“ in the “Rotor“ area using the left knob. An other rotor can be selected by turning the right knob. This is not possible during a centrifuge run.

Attention: An automatic rotor identification occurs after the centrifuge has

been started. If the used rotor is not the same than the preselected one, the used rotor is displayed and the preselected speed is corrected.

Preselected rotor 12158

Changed to rotor 12150

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5.1 Practical Notes for Centrifugation

1. Locate centrifuge horizontally on a level surface.

2. Ensure safe location.

3. Keep at least 30 cm free space around the centrifuge.

4. Provide for sufficient ventilation.

5. Tighten rotor firmly onto motor shaft.

6. Avoid imbalance.

7. Load opposite buckets with same accessories.

8. Centrifugation with low capacity:

The example shows a complete swing out rotor 16 x 15 ml which is loaded with 8 vessels only. The vessels must be

loaded symmetrically so that the buckets and their suspensions are loaded evenly. Loading an eg. only an outer position of the bucket is not allowed.

8.1Centrifugation with different tubes: Working with different tube sizes is possible. Opposite places must be loaded with the same vessels.The example shows a swing-out rotor with buckets and 2 x carriers 100 ml and 2 x carriers 4 x 15 ml with tubes.

8.2 Another example is the fixed angle rotor 24 x 2,2 ml:

The vessels should be placed symmetri-

cally so that the rotor is loaded evenly. Loading e.g. only one position is not allowed.

9. Load all positions of swing-out rotors.

10. Load vessels outside the centrifuge.

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11. Please pay attention to the max. speed. At speeds over 4 000 rpm there is an

increased breaking glass hazard.

12. Fill vessels carefully to same weight. Imbalances would result in increased wear of bearings.

13. Use perfect accessories only.

14. Avoid corrosion to accessories by careful maintenance.

15. Spin infectious material in sealed rotors and buckets only.

16. Do not spin explosive or highly imflammable materials.

17. When centrifuging substances with a density > 1,2 g/cm3 the allowable max. speed must be reduced (refer to chapter 7.1.2 "Density").

18. Grease joints of buckets and rotor pins in swing-out rotors.

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5.2 Forbidden Centrifuging Operations

1. Operation of not carefully installed centrifuge.

2. Operation without front or back panels.

3. Operation by non authorized personnel.

4. Operation with rotor not installed properly (refer to chapter 3.3).

5. Operation with incompletely loaded drum rotor, swing-out rotor or angle rotor with interchangeable buckets.

A rotor must always be loaded completely, empty places are not allowed!

Opposite buckets or carriers may nevertheless be empty. Mixed loading is allowed, if opposite places are loaded with same buckets and carriers of same weight.

6. Operation with overloaded rotors.

The load for a rotor is limited by its design and the max. speed (see

rotor/bucket engraving) and must not be exceeded. The rotors are intended for liquids of max. homogeneous density of 1.2 g/cm speed. If liquids of higher density are used, the speed must be reduced accordingly (refer to chapter 7.1 "Mathematical relations").

7. Operation with rotors and adapters showing corrosion or other defects.

8. Operation of very corrosive substances which can cause damages to material and affect mechanical strength of rotors and adapters.

9. Operation of rotors and accessories not allowed by the manufacturer. The use of poor commodity goods is not recommended. At high speeds breaking glass or bursting vessels can cause dangerous imbalances.

10. Operation in hazardous locations.

11. Operation with vessels of improper size.

12. Centrifugation of improper material.

13. Operation with partially filled plastic tubes in high-speed angle rotors.

14. Lifting or moving of the centrifuge during operation. Leaning against or resting on the centrifuge is not allowed.

15. Do not place potential dangerous material - e.g. glass vessels containing liquids - near the centrifuge.

if centrifuged at max.

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16. Attention: Do not open cover and/or reach into rotor chamber unless the rotor is at standstill. Never attempt to override the lid interlock system while the rotor is spinning.

17. Such materials are prohibited which chemically interact vigorously.

18. Do not spin explosive or inflammable materials.

19. Substances which could damage the material of the centrifuge, the rotors or the adapters must not be centrifuged. Infectious, toxic, pathogene or radioactive substances must be centrifuged in certified rotors and vessels only and all necessary safety precautions are taken.

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6.1 Care and Cleaning of Centrifuge

Please use water-soluble, mild detergents for cleaning. Avoid corroding and aggressive substances. Do not use alkaline solutions or solvents or agents with abrasive particles. Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge.

Remove product particles from the rotor chamber using a cloth or paper towl. It is recommended to open the cover when the centrifuge is not in use so that moisture can evaporate. Increased wear of the motor bearings will thus be avoided. If there is the risk of toxic, radioactive or pathogene

contamination, special safety measures must be kept.

6.2 Care and Cleaning of Accessories

For care of accessories special safety measures must be considered as these are measures ensuring operational safety at the same time.

Buckets, trunnions and also synthetic buckets are produced exactly in order to withstand the permanent high stress with high gravitational fields.

Chemical reactions as well as stress-corrosion (combination of oscillating pressure and chemical reaction) can affect or destroy the metals. Hardly detectable cracks on the surface expand and weaken the material without visible signs. When detecting a visible damage of the surface, a crack, a mark or any other change, also corrosion, the part (rotor, etc.) must be replaced immediately.

In order to avoid corrosion, rotor incl. tie-down screw and cover seal and adapters must be cleaned and greased regularly with the supplied slushing oil (Sigma part no.: 70104 for 20 ml slushing oil). Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge. The rotor tie-down screw must be greased using grease (Sigma part no.: 70284).

Cleaning of accessories should be done outside of the centrifuge once a week or preferably after every use. Adapters should be removed. After this the parts should be dried with a soft cloth or, alternatively, in a drying chamber at approx. 50 °C. If there is the risk of toxic, radioactive or pathogene contamination,

special safety measures must be kept.

Especially aluminium parts are extremely corrosive. A neutral cleaning detergent with a pH-value between 6 and 8 should be used for such parts. Alkaline agents exceeding pH 8 must be avoided. Especially aluminium parts must be greased regularly with slushing oil. This procedure essentially increases life time and reduces corrosion.

Careful maintenance increases life time and avoids premature failure of the rotor. Corrosion or resultant damages which are caused by insufficient care do not constitute a warranty claim.

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6.3 Rotor Pins

The trunnion pins of the rotor should always be greased as only this ensures evenly swinging of buckets and thus quiet run of the centrifuge (part no. 70284 Grease).

6.4 Glass Breakage

In case of glass breakage all glass particles must be carefully removed. Rubber inserts have to be cleaned carefully and possibly be replaced. If a problem has occured, the following has to be considered:

Glass particles in the rubber cushion will cause glass breakage again. Glass particles in the centrifuge chamber will cause metal abrasion due to the

strong air circulation. This dust will not only pollute the centrifuge chamber, the rotor and the material to be centrifuged but also damage the surfaces of the accessories, the rotors and the centrifuge chamber.

In order to totally remove the glass particles and the metal dust from the rotor chamber, it is advisable to grease the upper part of the centrifuge chamber with e.g. Vaseline. Then the rotor should rotate for some minutes at a moderate speed. The glass and metal particles will now collect at the greased part and can easily be removed with a cloth together with the grease. If necessary repeat this procedure.

6.5 Care and Cleaning of the Condenser

A lamellar condenser is used to cool the compressed refrigerant. It is installed in the back part of the centrifuge and cooled with air.

The location for the centrifuge should therefore be as clean as possible, ie. dirt, dust etc. should not prevent the air from streaming through the condenser and obstruct heat transfer. Dust on the condenser pipes and the lamella will reduce heat exchange and decrease the performance of the refrigerator.

The condenser should therefore be regularly checked for dirt and cleaned if necessary. Generally blowing of the lamella with compressed air from inside and outside is sufficient (or a simple hoovering with a vacuum cleaner).

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6.6 Sterilization and Disinfection of Rotor Chamber and Accessories

All usual disinfectants like eg. Sagrotan, Buraton or Terralin (to obtain at chemist’s shops) can be used. The centrifuges and the accessories consist of different materials. A possible incompatibility must be considered. Before using detergents or decontamination agents which had not been recommended by us, the user has to contact us to make sure that such procedure would not damage the centrifuge. For sterilization by steam resistance to temperature of the individual material must be checked (refer to point 6.6.1 “Autoclaving“). Please contact your laboratory safety officer regarding proper methods to use. If

dangerous materials are used, the centrifuge and the accessories must be disinfected.

Principally we want to point out that for centrifuging of e.g. infectious material certified and hermetically sealed accessories have to be used in order to avoid that the centrifuge is contaminated.

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6.6.1 Autoclaving

The life of the accessories essentially depends on the frequency of autoclaving and use. When the parts are showing changes in colour or structure or in the event of leaks etc., the accessories have to be replaced.

During autoclaving the caps of the tubes must not be screwed on to avoid deformation of the tubes. It can not be excluded that plastic parts, e.g. lids or carriers, would deform during autoclaving.

Autoclaving:

Accessories max. temp. Glass tubes 134-138 3 5 -

Polycarbonate tubes 115-118 30 40 20 Polypropylene tubes 115-118 30 40 30 Teflon tubes 134-138 3 5 100 Aluminium rotors 134-138 3 5 Polypropylene rotor 12034 115-118 30 40 20 Polypropylene rotor 12124 115-118 30 40 20 Polycarbonate/Polyallomer lids for angle rotors Polysulfone lids for angle rotors Aluminium buckets 134-138 3 5 Polycarbonate caps for buckets Polypropylene caps for buckets Polysulfone caps for buckets Rubber adapters 115-118 30 40 Rubber cushions 115-118 30 40 Round carriers for 13104/ 13117, Polypropylene ditto, Polyallomer and Polycarbonate Round carriers for 13350/ 13550, Polypropylene Rectangular carriers, Polypropylene ditto, Polyallomer and Polycarbonate

min. time

°C

115-118 30 40 20 134-138 3 5 100

115-118 30 40 50 115-118 30 40 50 134-138 3 5 100

115-118 30 40 115-118 30 40 115-118 30 40 115-118 30 40 115-118 30 40 -

min

max. time min

max. cycles

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6.7 Checks by Operator

The operator has to ensure that no important part of the centrifuge is damaged. This especially refers to:

1. Motor suspension

2. Concentricity of the motor shaft

3. Fastening of the trunnions in the rotor

4. Rotors and accessories have a limited life. For safety reasons a regular check is recommended after 50.000 cycles. Any changes like e.g. corrosion, cracks, material abrasion etc. require special attention.

5. Screw connections have to be tight.

Furthermore, the earth wire must be checked regularly.

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7.1 Mathematical Relations

7.1.1 Relative Centrifugal Force (RCF)

The parameters speed, RCF and the diameter of the rotor are interrelated via the following formula:

RCF = 11,18 x 10-6 x r x n2

If two values are given, the third value is determined by the equation. If the speed or the rotation radius is changed, the resulting RCF will be recalculated. If the RCF is changed, the speed under consideration of the radius is adapted accordingly.

r = radius in cm n = speed in rpm RCF without dimension

7.1.2 Density

If the density of the liquid is higher than 1.2 g/cm3, the allowed maximum speed of the centrifuge is reduced according to the following formula:

n = n

Rho = density in g/cm3

max

)/2,1( Rho

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7.2 Error Correction

Most of the errors can be reset by power off/on. In the event of a short power failure during a run, this run is interrupted and can be continued by pressing the Start-key.

No indication on the display: Actions:

− No power in the socket?

− Power cord plugged in and line

voltage present?

− Power switch on?

− Lid closed?

• Check fuse in mains supply.

• Plug in power cord correctly.

• Switch on power.

• Close lid (please refer to chapter 7.2.3 „Lid

cannot be opened/closed.“).

7.2.1 Centrifuge cannot be Started

− Start-key LED illuminated: • Power off/on. If error occurs again, call

service.

− Lid-key LED flashing: • Open and close the lid again. If error

occurs again although the lock engaged, call service.

7.2.2 Centrifuge Decelerates during Operation

− Centrifuge displays an error 1

to 11 after power on.

• Power off/on. If error occurs again, call service (please refer to chapter 7.3.1 "Error codes").

7.2.3 Lid cannot be Opened/Closed

− When first trying to open the lid the lock is not released. The Lid-key LED is flashing. Open and close lid again.

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7.2.4 Emergency Lid Release

At the upper right panel there is a stopper which can be removed eg. with a screw driver. The lid can be released by pulling the visible string.

Attention! The lid may only be unlocked and opened when the rotor is at standstill.

7.2.5 Problems with the Centrifuge?

Please contact your supplier for support or in the event of malfunctions and for supply of spare parts.

7.3 Error Mode

In the error mode the Start-, Stop- and Lid-keys are flashing. “ERROR“ is displayed in the speed area and the error code in the rcf area.

7.3.1 Error Codes

Error no. Kind of error Actions 1 Tacho signal disturbed 4 Lid does not open after

pressing the Lid-key 2, 3, 5 – 11, 17 - 30 Internal fault 12 Imbalance determined 13 Temperature sensor 14 Excess temperature (>50°C)

Should it not be possible to repair the failure, please contact Service!

7.4 Speed-RCF-Diagram

An additional help is the enclosed Speed-RCF-Diagram.

• Power off/on

• Emergency lid release

• Power off/on

• Remove imbalance

• Contact service

• Let centrifuge cool down

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7.5 Declaration of Decontamination / Return Declaration

The following declarations serve for keeping safety and health of our employees. Fill in the forms and attach them when returning centrifuges, accessories and spare parts. Please understand that we cannot carry out any work before we have the declarations. (We recommend to make several copies of this page.)

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----------------------------------------------------------------------------------------------------------------------

Decontamination declaration inside :

Unit / component contaminated :

Unit / component unused (new) :

---------------------------------------------------------------------------------------------------------------------Please make some copies before removing this page.

!!! Attention – This form must be glued on outside of the box !!!

Return declaration

YES NO

!!! Attention – This form must be glued on outside of the box !!!

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Declaration of Decontamination of Centrifuges, Accessories and Spare Parts

This declaration may only be filled in and signed by authorised staff.

Repair Order dtd. : Order No. : Type of unit : Serial No. :

Type of unit : Serial No. : Type of unit : Serial No. : Type of unit : Serial No. :

Accessories :

Is the equipment free from harmful substances ? YES Ο NO Ο If not, which substances have come into contact with the equipment? Name of the substances : _________________________________________________________________

_________________________________________________________________

Remarks (e.g to be touched _________________________________________________________________ with gloves only) : _________________________________________________________________

General characteristics of the substances : Corrosive Ο Explosive Ο Biologically hazardous Ο Radioactive Ο Toxic Ο

In combination with which substances may hazardous mixtures develop? Name of the substances :

Has the equipment been cleaned before shipment? YES Ο NO Ο Is the equipment decontaminated and not harmful to health? YES Ο NO Ο Prior to repair, radioactively contaminated components must be decontaminated according to the valid

regulations for radiation protection.

Legally Binding Declaration

I / we hereby declare that the information on this declaration are correct and complete. Company / Institute : Street : Postcode, City : Tel. : FAX : Name :

Date : Stamp :

Signature :

---------------------------------------------------------------------------------------------------------------------Please make some copies before removing this page.

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