Sigma 2-16P Operating Manual

Translation from the original operating manual
sb

04/2007
Rev. 2.15 of 15/09/2017
Sigma 2-16P; ;

part no. 0700602

Sigma 2-16P

from serial no. 120756

Laboratory Centrifuge

Operating Manual

Please retain for later use!

Laboratory Centrifuge Sigma 2-16P

2 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Laboratory Centrifuge Sigma 2-16P

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

3 / 72

Translation from the original operating manual, part no. 0700602

In case of inquiries, please state the following numbers:

Order number:
Serial number:

© Copyright by
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany

Tel.: [phone] / 5007-0
Fax: [phone] / 5007-12
Web: www.sigma-zentrifugen.de
E-mail: [email protected]

Laboratory Centrifuge Sigma 2-16P

4 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Laboratory Centrifuge Sigma 2-16P

Table of contents

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

5 / 72

Translation from the original operating manual, part no. 0700602

1 General information ............................................................................................................. 9

1.1 Importance of the operating manual .................................................................................. 9

1.2 Intended use ..................................................................................................................... 9

1.3 Warranty and liability......................................................................................................... 9

1.4 Copyright ........................................................................................................................ 10

1.5 Standards and regulations .............................................................................................. 10

1.6 Scope of supply .............................................................................................................. 10

2 Layout and mode of operation .......................................................................................... 11

2.1 Layout of the centrifuge .................................................................................................. 11

2.1.1 Functional and operating elements ............................................................................ 11

2.1.2 Name plate ................................................................................................................ 12

2.2 Mode of operation ........................................................................................................... 13

2.2.1 Centrifugation principle .............................................................................................. 13

2.2.2 Area of application ..................................................................................................... 13

2.2.2.1 Speed, radius, and relative centrifugal force ...................................................... 14

2.2.2.2 Density ............................................................................................................... 14

3 Safety .................................................................................................................................. 15

3.1 Marking of the unit ................................ .......................................................................... 15

3.2 Explanation of the symbols and notes ............................................................................. 16

3.3 Responsibility of the operator .......................................................................................... 17

3.4 Operating personnel ....................................................................................................... 17

3.5 Informal safety instructions ............................................................................................. 17

3.6 Safety instructions .......................................................................................................... 18

3.6.1 Electrical safety ......................................................................................................... 18

3.6.2 Mechanical safety ...................................................................................................... 18

3.6.3 Fire prevention ........................................................................................................... 19

3.6.4 Chemical and biological safety .................................................................................. 19

3.6.5 Safety instructions for centrifugation .......................................................................... 20

3.6.6 Resistance of plastics ................................................................................................ 20

3.6.7 Safety of rotors and accessories ................................................................................ 21

3.6.7.1 Service life ................................................................ ......................................... 21

3.7 Safety devices ................................................................................................................ 22

3.7.1 Lid lock device ........................................................................................................... 22

3.7.2 Standstill monitoring system ...................................................................................... 22

3.7.3 System check ............................................................................................................ 22

3.7.4 Earth conductor check ............................................................................................... 22

3.7.5 Imbalance monitoring system .................................................................................... 22

3.8 Measures in the event of hazards and accidents ............................................................ 23

3.9 Remaining hazards ......................................................................................................... 23

Laboratory Centrifuge Sigma 2-16P

Table of contents

6 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

4 Storage and transport ....................................................................................................... 24

4.1 Dimensions and weight ................................................................................................... 24

4.2 Storage conditions .......................................................................................................... 24

4.3 Notes on transport ................................ .......................................................................... 24

4.4 Packaging ....................................................................................................................... 25

4.5 Transport safety device ................................................................................................... 25

5 Set-up and connection ...................................................................................................... 26

5.1 Installation site ................................................................................................................ 26

5.2 Power supply .................................................................................................................. 26

5.2.1 Type of connection .................................................................................................... 26

5.2.2 Customer-provided fuses ........................................................................................... 26

6 Using the centrifuge .......................................................................................................... 27

6.1 Initial start-up .................................................................................................................. 27

6.2 Switching the centrifuge on ............................................................................................. 27

6.2.1 Opening and closing the lid ........................................................................................ 27

6.2.2 Installation of rotors and accessories ......................................................................... 28

6.2.2.1 Installation of the rotor ........................................................................................ 28

6.2.2.2 Installation of angle rotors with a hermetically sealed lid .................................... 29

6.2.2.3 Installation of the microhaematocrit rotor............................................................ 30

6.2.2.4 Installation of accessories .................................................................................. 31

6.2.2.5 Adapters ............................................................................................................ 32

6.2.2.6 Tubes ................................ ................................................................ ................. 32

6.3 Control system "Spincontrol Universal" ........................................................................... 33

6.3.1 User interface ............................................................................................................ 33

6.3.2 Manual mode ............................................................................................................. 34

6.3.2.1 Starting a centrifugation run ............................................................................... 34

6.3.2.2 Interrupting a centrifugation run ......................................................................... 34

6.3.2.3 Interrupting a deceleration process .................................................................... 34

6.3.2.4 Selection, display, and modification of data ........................................................ 34

6.3.2.5 Runtime ............................................................................................................. 34

6.3.2.6 Speed ................................................................................................................ 36

6.3.2.7 Relative centrifugal force (RCF) ......................................................................... 36

6.3.2.8 Rotor selection ................................................................................................... 37

6.3.2.9 Softstart and softstop function ............................................................................ 37

6.3.2.10 Automatic lid opening function ("Auto-Lid-Open") ............................................... 38

6.3.2.11 Sound signal ("Buzzer") ..................................................................................... 38

6.3.2.12 Input lock ........................................................................................................... 38

6.3.3 Programs ................................................................................................................... 39

6.3.3.1 Saving the current settings ................................................................................. 39

6.3.3.2 Calling up stored programs ................................................................................ 39

6.4 Switching the centrifuge off ............................................................................................. 39

Laboratory Centrifuge Sigma 2-16P

Table of contents

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

7 / 72

Translation from the original operating manual, part no. 0700602

7 Malfunctions and error correction .................................................................................... 40

7.1 General malfunctions ...................................................................................................... 40

7.1.1 Emergency lid release ............................................................................................... 41

7.2 Table of error codes ........................................................................................................ 42

7.3 Service contact ............................................................................................................... 43

8 Maintenance and service ................................................................................................... 44

8.1 Maintenance ................................................................................................................... 44

8.1.1 Centrifuge .................................................................................................................. 44

8.1.2 Accessories ............................................................................................................... 45

8.1.2.1 Plastic accessories ............................................................................................ 45

8.1.3 Rotors, buckets and carriers ...................................................................................... 46

8.1.4 Microhaematocrit rotor ............................................................................................... 46

8.1.5 Load bearing bolts ................................................................................................ ..... 46

8.1.6 Glass breakage ......................................................................................................... 47

8.2 Sterilisation and disinfection of the rotor chamber and accessories ................................ 47

8.2.1 Autoclaving ................................................................................................................ 48

8.3 Service ........................................................................................................................... 49

8.4 Return of defective parts ................................................................................................. 50

9 Disposal .............................................................................................................................. 52

9.1 Disposal of the centrifuge ............................................................................................... 52

9.2 Disposal of the packaging ............................................................................................... 52

10 Technical data .................................................................................................................... 53

10.1 Ambient conditions ......................................................................................................... 53

10.2 Technical documentation ................................................................................................ 54

11 Appendix ............................................................................................................................ 55

11.1 Range of accessories ..................................................................................................... 55

11.1.1 Maximum speed for tubes .......................................................................................... 57

11.1.2 Rotor radii .................................................................................................................. 58

11.2 Speed-gravitational-field-diagram ................................ ................................................... 59

11.3 Table of the service life of rotors and accessories ........................................................... 60

11.4 Resistance data .............................................................................................................. 61

11.5 EC declaration of conformity ........................................................................................... 65

11.6 Declaration of conformity – China RoHS 2 ...................................................................... 67

12 Index ................................................................................................................................... 69

Laboratory Centrifuge Sigma 2-16P

Table of contents

8 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Laboratory Centrifuge Sigma 2-16P

1 General information

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

9 / 72

Translation from the original operating manual, part no. 0700602

Pos: 1 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/010 Allgemeine Informationen/010 Allgemeine Infor mationen ================= =================== @ 26\mod_14053189 48131_68.docx @ 191680 @ 1 @ 1

1 General information

Pos: 2 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/010 Allgemein e Informationen/010-0010 Stellenwert der Betriebsanleitung------------------------------------------------------- @ 26\mod_1405318949962_68.docx @ 191694 @ 2 @ 1

1.1 Importance of the operating manual

A fundamental requirement for the safe and trouble-free operation of the
centrifuge is to be familiar with the fundamental safety instructions and all
possible hazards.

The operating manual includes important information concerning the safe
operation of the centrifuge.

This operating manual and, in particular, the notes on safety and hazards
must be observed by all persons operating the centrifuge.

In addition, the local rules and regulations for the prevention of accidents

Pos: 3 /010 Universalmodule/ Leerzeile @ 0 \mod_1202116244500_0.docx @ 114 @ @ 1

Pos: 4 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/010 Allgemeine Informationen/010-0020 Bestimmungsg emäße Verwendung------------------------------------------- @ 26\mod_1405318951312_68.doc x @ 191709 @ 2 @ 1


must be complied with.

1.2 Intended use

Centrifuges are power-driven machines that separate liquids from solid
matter, liquid mixtures, or solid mixtures by centrifugal force. They are
solely intended for this purpose. Any other use beyond this area of
application is regarded as improper use. Sigma Laborzentrifugen GmbH
cannot be held liable for any damage resulting from such improper use.

The intended use also includes

• observation of all the notes and instructions included in the operating
manual and

Pos: 5 /010 Universalmodule/ Leerzeile @ 0 \mod_1202116244500_0.docx @ 114 @ @ 1

Pos: 6 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/010 Allgemein e Informationen/010-0030 Gewährleistu ng und Haftung-------------------------------------------------- @ 26\mod_1405318955234_68.docx @ 191737 @ 2 @ 1


• compliance with the care, cleaning, and maintenance instructions.

1.3 Warranty and liability

The warranty and liability are subject to our "General Conditions" that were
distributed to the operator upon the conclusion of the contract.

Warranty and liability claims are excluded if they are due to:

• improper use.

• non-compliance with the safety instructions and hazard warnings in the

operating manual.

• improper installation, start-up, operation, or maintenance of the

Pos: 7 /010 Universalmodule/Seitenwechs el @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

centrifuge.

Laboratory Centrifuge Sigma 2-16P

1 General information

10 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

• 1 power cord with an IEC C13 connector

depending on the voltage
variant

• 1 rotor wrench

Part no. 930 100

• 1 tube (30 g) heavy-duty grease for load-
bearing bolts

Part no. 71 401

Pos: 8 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/010 Allgemeine Informationen/010-0040 Urheberrecht----------------------------------------------------------------- @ 26\mod_1405318956858_68.docx @ 191751 @ 2 @ 1


1.4 Copyright

The copyright concerning the operating manual remains with
Sigma Laborzentrifugen GmbH.

The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that must not be

• duplicated,

• distributed, or

• communicated in any other way.

Pos: 9 /010 Universalmodule/ Leerzeile @ 0 \mod_1202116244500_0.docx @ 114 @ @ 1

Pos: 10 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/010 Allgemein e Informationen/010-0050 Normen u nd Vorschriften------------------------------------------------------ @ 26\mod_1405318958202_68.docx @ 191765 @ 2 @ 1


Non-compliance may be prosecuted under criminal law.

1.5 Standards and regulations

EC declaration of conformity

Pos: 11 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 12 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/010 Allgemein e Informationen/010-0060 Lieferumfang 2-16P @ 29\mod_1405321487553_6 8.docx @ 196875 @ 2 @ 1

(see chapter 11.5 - "EC declaration of conformity")

1.6 Scope of supply

The centrifuge comprises:

Documentation

Operating manual incl. EC declaration of conformity
(see chapter 11.5 - "EC declaration of conformity")

Accessories

Pos: 13 /010 Universalmodule/Abschnitts wechsel @ 0\mod_1202124514062_0.d ocx @ 418 @ @ 1

Pos: 14 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

According to your order, our order confirmation, and your delivery note.

Laboratory Centrifuge Sigma 2-16P

2 Layout and mode of operation

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

11 / 72

Translation from the original operating manual, part no. 0700602

1 Lid
2 User interface (see

chapter 6.3.1 - "User
interface")

3 Mains switch

4 Name plate

(see chapter 2.1.2 ­"Name plate")

5 Equipotential bonding

screw

6 Mains power input

Pos: 15 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020 Aufbau und Wir kungsweise================================== @ 26\mod_1405318981820_68.docx @ 191779 @ 1 @ 1


2 Layout and mode of operation

Pos: 16 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020-0010 Aufbau d er Zentrifuge---------------------------------------------------- @ 26\mod_1405318982945_68.docx @ 191793 @ 2 @ 1


2.1 Layout of the centrifuge

Pos: 17 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/020 Aufbau und Wirkungsweise/020-0010-0010 Fun ktions- und Bedienelemente 2-16P @ 29\ mod_1405321495781_68.docx @ 1968 89 @ 3 @ 1

2.1.1 Functional and operating elements

Pos: 18 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Fig. 1: Total view of the centrifuge

Fig. 2: Rear view of the centrifuge

Laboratory Centrifuge Sigma 2-16P

2 Layout and mode of operation

12 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

1 Manufacturer and

registered office
2 Type
3 Serial number
4 Max. density
5 Nominal voltage
6 Input fuse
7 Symbol for special

disposal (see chapter

9 - "Disposal")
8 CE-mark in

compliance with the

directive 2006/42/EG
9 Part number
10 Year of manufacture
11 Max. speed
12 Max. kinetic energy
13 Power consumption

Pos: 19 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/020 Aufbau und Wirkungsweise/020-0010-0020 T ypenschild 2-16P @ 29\mod_14053214966 34_68.docx @ 196903 @ 3 @ 1

2.1.2 Name plate

Pos: 20 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Fig. 3: Example of a name plate

Laboratory Centrifuge Sigma 2-16P

2 Layout and mode of operation

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

13 / 72

Translation from the original operating manual, part no. 0700602

Pos: 21 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020-0020 Wirkungs weise---------------------------------------------------------- @ 26\mod_1405318984194_68.docx @ 191807 @ 2 @ 1


2.2 Mode of operation

Pos: 22 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020-0020-0010 Pri nzip der Zentrifugation @ 26\mod_14 05318985448_68.docx @ 191821 @ 3 @ 1


2.2.1 Centrifugation principle

Pos: 23 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 24 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020-0020-0020 An wendungsbereich @ 26\mod_140531 8986763_68.docx @ 191835 @ 3 @ 1
Das

Centrifugation is a process for the separation of heterogeneous mixtures of
substances (suspensions, emulsions, or gas mixtures) into their
components. The mixture of substances, which rotates on a circular path, is
subject to centripetal acceleration that is several times greater than the
gravitational acceleration.

Centrifuges use the mass inertia inside the rotor chamber for separating the
substances. Due to their higher inertia, particles or media with a higher
density travel outwards. In doing so, they displace the components with a
lower density, which in turn travel towards the centre.

The centripetal acceleration of an object inside a centrifuge, as the effect of
centripetal force, depends on the distance between the object and the axis
of rotation as well as on the angular velocity. It increases linearly as a
function of the distance with regard to the axis of rotation and quadratically
as a function of the angular velocity. The bigger the radius in the rotor
chamber is and the higher the speed is, the higher the centripetal
acceleration is. However, the forces acting on the rotor also increase.

2.2.2 Area of application

Depending on the area of application of the centrifuge and also on the
particle size, solids content, and volume throughput of the mixture of
substances that is to be centrifuged, there are different types of centrifuges.

The areas of application go from household use as a salad spinner or
honey separator up to specialised technical applications in the clinical,
biological, or biochemical context:

• For numerous clinical examinations, cellular material must be separated
from the liquid to be analysed. The normal separation process can be
sped up considerably by using laboratory centrifuges.

• In the metal-working industry, centrifuges are used for separating oil

from metal cuttings. Dairies use centrifuges in order to separate cow’s

milk into cream and low-fat milk.

• Particularly big centrifuges are used in the sugar industry for separating
the syrup from the crystalline sugar.

• Ultracentrifuges are predominantly used in biology and biochemistry in
order to isolate particles, e.g. viruses. They are specifically designed for
high speeds up to 500,000 rpm. The rotor moves in a vacuum in order

Pos: 25 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

to avoid air friction.

Laboratory Centrifuge Sigma 2-16P

2 Layout and mode of operation

14 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

)/2,1( Rho

Pos: 26 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020-0020-0020-001 0 Drehzahl, Radius, Relative Zentrifugalb eschleunigung @ 26\mod_1405318 988082_68.docx @ 191849 @ 4 @ 1


2.2.2.1 Speed, radius, and relative centrifugal force

The acceleration g, which the samples are subject to, can be increased by
increasing the radius in the rotor chamber and by increasing the speed.
These three parameters are interdependent and linked with each other via
the following formula:

Relative centrifugal force RCF = 11.18 x 10-6 x r x n2

r = radius in cm
n = speed in rpm
RCF without any dimension

If two values are entered, the third value is determined by way of the stated
formula. If, afterwards, the speed or the radius is changed, the resulting
relative centrifugal force will be recalculated automatically by the control
unit. If the RCF is changed, the speed will be adapted while the specified
radius is maintained.

The speed-gravitational-field-diagram provides an overview of the
relationship between speed, radius, and RCF (see chapter 11.2 - "Speed-

Pos: 27 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 28 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/020 Aufbau und Wirkungsweise/020-0020-0020-002 0 Dichte @ 26\mod_1405318989359_6 8.docx @ 191863 @ 4 @ 1


gravitational-field-diagram").

2.2.2.2 Density

The laboratory centrifuge is suitable for the separation of constituents of
different densities in mixtures with a maximum density of 1.2 g/cm3. All
information concerning the speed of rotors and accessories refers to liquids
with a density corresponding to this specification. If the density is above this
value, the maximum permissible speed of the centrifuge must be reduced
based on the following formula:

n = n

Pos: 29 /010 Universalmodule/Abschnitts wechsel @ 0\mod_1202124514062_0 .docx @ 418 @ @ 1

Pos: 30 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Rho = density in g/cm3

max

x

Laboratory Centrifuge Sigma 2-16P

3 Safety

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

15 / 72

Translation from the original operating manual, part no. 0700602

Dangerous voltage

I

On (Power)

Hot surface

0

Off (Power)

Caution! Risk of bruising

Name plate (see chapter

2.1.2 - "Name plate")

Protective earth (ground)

CE mark in compliance with
the directive 2006/42/EC

Earth (ground)

Do not dispose as part of
domestic waste

Unplug the mains plug

GS mark (tested safety;
only for Germany)

Arrow indicating the
direction of rotation

NRTL mark (only for the
USA and Canada)

4°C Guarantee

RCM mark
(only for Australia)

Centrifuge with heating
system

China RoHS 2 mark
(only for China)

NOTE

Safety indications on the centrifuge must be kept readable at all times. If
necessary, they must be replaced.

NOTE

Not all of the symbols/labels are used for this centrifuge type.

Pos: 31 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30 Sicherheit=========== ============================ @ 26\m od_1405319007254_68.docx @ 191877 @ 1 @ 1


3 Safety

Pos: 32 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0010 Beschilderung des Geräts ( außer 3-30KS) @ 30\mod_140540 4002477_68.docx @ 203921 @ 2 @ 1


3.1 Marking of the unit

The following symbols are used for all types of centrifuges manufactured by Sigma:

Pos: 33 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Laboratory Centrifuge Sigma 2-16P

3 Safety

16 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

DANGER

This symbol stands for a direct hazard to the life and health of persons.

Non-observance of these symbols causes serious health problems up to
life-endangering injuries.

DANGER

This symbol stands for a direct hazard to the life and health of persons
due to electrical voltage.

Non-observance of these symbols causes serious health problems up to
life-endangering injuries.

WARNING

This symbol stands for a potential hazard to the life and health of persons.

Non-observance of these symbols can cause serious health problems up
to life-endangering injuries.

CAUTION

This symbol indicates a potentially hazardous situation

Non-observance of these notes can cause minor injuries or damage to
property.

NOTE

This symbol indicates important information.

Pos: 34 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 35 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0020 Symbol- und Hinweiser klärungen @ 26\mod_1405319013955_68. docx @ 191947 @ 2 @ 1


3.2 Explanation of the symbols and notes

In this operating manual, the following names and symbols to indicate
hazards are used:

Pos: 36 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

3 Safety

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

17 / 72

Translation from the original operating manual, part no. 0700602

Pos: 37 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0030 Verantwortung des Betrei bers @ 26\mod_1405319015364_68.doc x @ 191961 @ 2 @ 1


3.3 Responsibility of the operator

The operator is responsible for authorising only qualified personnel to work
on the centrifuge (see chapter 3.4 - "Operating personnel").

The areas of responsibility of the personnel concerning the operation,
maintenance, and care of the unit must be clearly defined.

The safety-conscious work of the personnel in compliance with the
operating manual and the relevant EC and national health and safety
regulations as well as with the accident prevention regulations must be
checked at regular intervals (e.g. every month).

Under the international rules for health and safety at work, the operator is
obliged to:

• take measures in order to prevent all danger to life or health during
work.

• ensure that centrifuges are operated properly and entirely as intended
(see chapter 1.2 - "Intended use").

• take protective measures against fire and explosion when working with
hazardous substances.

Pos: 38 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 39 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0040 Bedienpersonal @ 26\mo d_1405319017634_68.docx @ 191989 @ 2 @ 1


• take measures for the safe opening of centrifuges.

3.4 Operating personnel

Persons operating the unit must

• be familiar with the fundamental regulations concerning workplace
safety and accident prevention

• have read and understood this operating manual (and in particular the
safety sections and warning notes) and confirmed this with their

Pos: 40 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 41 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0050 Informelle Sicherheitshin weise @ 26\mod_1405319019966_68.d ocx @ 192017 @ 2 @ 1


signature.

3.5 Informal safety instructions

• This operating manual is a part of the product.

• The operating manual must be kept at the location of use of the

centrifuge. Ensure that it is accessible at all times.

• The operating manual must be handed over to any subsequent owner
or operator of the centrifuge.

• Any changes made must be added to the operating manual.

• In addition to the operating manual, the general and local rules and

regulations concerning the prevention of accidents and the protection of
the environment must also be supplied.

• Safety and danger indications on the centrifuge must be kept readable

Pos: 42 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0. docx @ 105 @ @ 1

at all times. If necessary, they must be replaced.

Laboratory Centrifuge Sigma 2-16P

3 Safety

18 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

DANGER

• Ensure that the wall socket is properly wired and grounded.

• Check that the mains voltage agrees with the nominal voltage listed on

the name plate.

• Do not place vessels containing liquid on the centrifuge lid or within the
safety distance of 30 cm around the centrifuge. Spilled liquids may get
into the centrifuge and damage electrical or mechanical components.

• Work on the power supply system must only be performed by certified
electricians.

• Inspect the electrical equipment of the unit regularly. Defects such as
loose or burnt cables must be eliminated immediately.

WARNING

• Do not open the lid when the rotor is in motion!

• Do not reach into the rotor chamber when the rotor is in motion!

• Do not use the centrifuge if it was installed incorrectly.

• Do not use the centrifuge without panels.

• Do not use the centrifuge if the rotors and inserts show signs of

corrosion or other defects.

• Only use the centrifuge with rotors and accessories that have been
approved by the manufacturer. In case of doubt, contact the
manufacturer (see chapter 7.3 - "Service contact").

• Do not hold your fingers between the lid and the housing when closing
the lid. Risk of crushing!

• Defective lid relieving devices could cause the centrifuge lid to fall
(contact the service department, if necessary). Risk of crushing!

• Do not hit or move the centrifuge during its operation.

• Do not lean against or rest on the centrifuge during its operation.

• Do not spin any substances that could damage the material of the

rotors and buckets of the centrifuge in any way. Highly corrosive
substances, for example, damage the material and affect the
mechanical strength of the rotors and buckets.

• Stop the centrifuge immediately in the event of a malfunction. Eliminate
the malfunction (see chapter 7 - "Malfunctions and error correction") or
inform the service department of the manufacturer (see chapter 7.3 ­"Service contact").

• Ensure that all repairs are performed only by authorised and
specialised personnel.

Pos: 43 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060 Sicherheitshinweise------------------------------------------------------------- @ 26\mod_1405319021480_68.docx @ 192031 @ 2 @ 1


3.6 Safety instructions

Pos: 44 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0010 Elektrische Sicher heit @ 26\mod_1405319022891_68.doc x @ 192045 @ 3 @ 1


3.6.1 Electrical safety

To reduce the risk of electrical shock, the centrifuge uses a three-wire
electrical cord and plug to connect the equipment to earth-ground. To

Pos: 45 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 46 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0020 Mechanische Sicher heit (außer 1-16/1-16K) @ 26\mod_1 405319024314_68.docx @ 192059 @ 3 @ 1


preserve this safety feature:

3.6.2 Mechanical safety

In order to ensure the safe operation of the centrifuge, observe the
following:

Laboratory Centrifuge Sigma 2-16P

3 Safety

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

19 / 72

Translation from the original operating manual, part no. 0700602

WARNING

• Prior to any start-up, check the centrifuge, rotor, and accessories for
signs of damage that can be discerned from the outside. Special
attention must be paid to all of the rubber parts (e.g. motor cover, lid
seal, and adapters) in terms of visible structural changes. Defective
parts must be replaced immediately.

• Open the centrifuge when it is not in use so that moisture can
evaporate.

DANGER

• Do not spin explosive or inflammable substances.

• Do not use the centrifuge within hazardous locations.

DANGER

• Infectious, toxic, pathogenic, and radioactive substances may only be
used in special, certified containment systems with a bio-seal in order
to prevent the material from being released.

• Take suitable precautions for your own safety if there is a risk of toxic,
radioactive, or pathogenic contamination

• Materials that chemically react with each other with a high level of
energy are prohibited.

WARNING

• Keep informed about local measures to avoid harmful emissions
(depending on the substances to be centrifuged).

• Protective clothing is not required for the operation of the centrifuge.
The materials to be centrifuged may, however, require special safety
measures (e.g. centrifugation of infectious, toxic, radioactive, or
pathogenic substances).

Pos: 47 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 48 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0030 Brandschutz thermisc he Sicherungen @ 26\mod_14053190 25698_68.docx @ 192073 @ 3 @ 1


3.6.3 Fire prevention

Pos: 49 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 50 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0040 Chemische und bi ologische Sicherheit @ 26\mod_14053190 28141_68.docx @ 192101 @ 3 @ 1


3.6.4 Chemical and biological safety

If pathogenic, toxic, or radioactive samples are intended to be used in the
centrifuge, it is in the responsibility of the user to ensure that all necessary
safety regulations, guidelines, precautions, and practices are adhered to
accordingly.

Pos: 51 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

3 Safety

20 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

WARNING

• Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up
and connection").

• Maintain a safety distance of at least 30 cm (12 inches) around the
centrifuge.

• Do not store any dangerous goods in the centrifuge area.

• Do not stay in the safety area longer than what is absolutely necessary

for the operation of the centrifuge.

• Only use the centrifuge with rotors and accessories that have been
approved by the manufacturer. We explicitly warn against the use of
equipment of poor quality. Breaking glass or bursting vessels can
cause dangerous imbalances at high speeds

• Ensure that rotor and buckets are correctly fitted (see chapter 6.2.2.1 ­"Installation of the rotor").

• Observe the instructions on the installation of accessories (see chapter

6.2.2.4 - "Installation of accessories").

WARNING

• The rotor must be loaded axial symmetrically at equal weights.

• If liquids with a density > 1.2 g/cm3 are used, reduce the speed (see

chapter 2.2.2.2 - "Density").

• Do not use the centrifuge if the rotor is loaded asymmetrically.

• Do not use the centrifuge with tubes that are excessively long.

NOTE

• Refer to the resistance data (see chapter 11.4 - "Resistance data")!

Pos: 52 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0050 Sicherheitshinweise z ur Zentrifugation @ 26\mod_1405319 029632_68.docx @ 192115 @ 3 @ 1


3.6.5 Safety instructions for centrifugation

For safe operation, observe the following before starting the centrifuge:

Pos: 53 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 54 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0060 Beständigkeit von K unststoffen @ 26\mod_1405319031161_ 68.docx @ 192129 @ 3 @ 1


3.6.6 Resistance of plastics

Chemical influences have a strong effect on the polymeric chains of
plastics, and, therefore, on their physical properties. Plastic parts can be

Pos: 55 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

damaged if solvents, acids, or alkaline solutions are used.

Laboratory Centrifuge Sigma 2-16P

3 Safety

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

21 / 72

Translation from the original operating manual, part no. 0700602

WARNING

• Perform regular checks (at least once per month) for safety reasons!

• Pay special attention to changes, such as corrosion, cracks, material

abrasion, etc.

• After 10 years, they must be inspected by the manufacturer.

• After 50,000 cycles, the rotor must be scrapped for reasons of safety.

• If other data concerning the service life are engraved on the rotor or

bucket, these data shall apply accordingly. For example, a bucket with

the engraving “max. cycles = 10,000” has a service life of 10,000
cycles, and a rotor with the engraving “Exp. date 02/20” must be

scrapped in February 2020 at the latest (see figure).

NOTE

• Refer to the table of rotors and accessories with a different service
life (see chapter 11.3 - "Table of the service life of rotors and
accessories")!

Pos: 56 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0070 Sicherheit von Rotore n und Zubehör @ 44\mod_1470119200568_ 68.docx @ 437008 @ 3 @ 1

3.6.7 Safety of rotors and accessories

Pos: 57 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0060-0070-0010 Lebensdauer @ 26\mod_1405319032612_68.doc x @ 192143 @ 4 @ 1

3.6.7.1 Service life

The rotors and accessories have a limited service life.

Fig. 4: Different service life – engraving on the bucket/rotor

Pos: 58 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

3 Safety

22 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Pos: 59 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0070 Sicherheitseinrichtungen----------------------------------------- @ 27\mod_1405319034809_68.docx @ 192157 @ 2 @ 1


3.7 Safety devices

Pos: 60 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0070-0010 Deckelverriegelung @ 27\mod_1405319036321_68.docx @ 192171 @ 3 @ 1


3.7.1 Lid lock device

Pos: 61 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 62 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0070-0020 Stillstandsüberwach ung @ 27\mod_1405319037803_68.d ocx @ 192185 @ 3 @ 1


3.7.2 Standstill monitoring system

Pos: 63 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 64 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0070-0032 Systemkontrolle Spin control Basic+Easy+Universal @ 27\ mod_1405319041777_68.docx @ 19222 7 @ 3 @ 1

3.7.3 System check

Pos: 65 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 66 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0070-0040 Schutzleiterprüfung Spincontrol L+S+Easy+Universal @ 27\ mod_1405319043129_68.docx @ 192 241 @ 3 @ 1


The centrifuge can only be started when the lid is properly closed. The
electrical lock must be locked. The lid can only be opened when the rotor
has stopped. If the lid is opened by way of the emergency release system
during operation, the centrifuge will immediately switch off and decelerate
brakeless. If the lid is open, the drive is completely separated from the
mains power supply, i.e. the centrifuge cannot be started (see chapter 7.1.1

- "Emergency lid release").

Opening of the centrifuge lid is only possible if the rotor is at a standstill.
This standstill is checked by the microprocessor.

An internal system check monitors the data transfer and sensor signals with
regard to plausibility. Malfunctions are indicated by error messages with a
number in the speed/rcf display (see chapter 7.2 - "Table of error codes").

3.7.4 Earth conductor check

For the earth conductor check, there is an equipotential bonding screw on
the rear panel of the centrifuge (see chapter 2.1.1 - "Functional and
operating elements"). An earth conductor check can be carried out by
authorized and specialized personnel using a suitable measuring
instrument. Please contact the service department (see chapter 7.3 -

Pos: 67 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 68 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicher heit/030-0070-0051 Unwuchtüberwachung Spincontrol L+Easy+Universal @ 27\ mod_1405319047031_68.docx @ 1922 83 @ 3 @ 1


"Service contact").

3.7.5 Imbalance monitoring system

The indication "Imbalance" in the rotor field and, in some cases, also a
sound signal indicate that the centrifuge is in the impermissible imbalance
range.The drive will be switched off in the acceleration phase or during the

Pos: 69 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

run.

Laboratory Centrifuge Sigma 2-16P

3 Safety

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

23 / 72

Translation from the original operating manual, part no. 0700602

DANGER

• If an emergency arises, switch off the centrifuge immediately!

• If in doubt, call the emergency doctor!

Pos: 70 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0080 Verhalten bei Gefahren u nd Unfällen @ 27\mod_1405319051188 _68.docx @ 192325 @ 2 @ 1


3.8 Measures in the event of hazards and accidents

Pos: 71 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 72 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/030 Sicherheit/0 30-0100 Restrisiken @ 27\mod_14 05319052885_68.docx @ 192339 @ 2 @ 1


3.9 Remaining hazards

The unit was built state- of- the- art and according to the accepted safety
rules. However, danger to life and limb of the operator, or of third parties, or
impairments of the unit or other material assets cannot be completely
excluded when the unit is being used.

• Use the unit only for the purpose that it was originally intended for (see
chapter 1.2 - "Intended use").

• Use the unit only if it is in a perfect running state.

Pos: 73 /010 Universalmodule/Abschnitts wechsel @ 0\mod_1202124514062_0.d ocx @ 418 @ @ 1

Pos: 74 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

• Immediately eliminate any problems that can affect safety.

Laboratory Centrifuge Sigma 2-16P

4 Storage and transport

24 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Sigma 2-16P

Height:

300 mm

Height with open lid:

610 mm

Width:

365 mm

Depth:

452 mm

Weight:

32 kg

CAUTION

The centrifuge weighs approx. 32 kg!

Pos: 75 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/040 Lagerung und Transport/040 Lagerung und Trans port========================== @ 27\mod_1405319160149_68.docx @ 1923 53 @ 1 @ 1


4 Storage and transport

Pos: 76 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/040 Lagerung u nd Transport/040-0010 Abmessunge n und Gewicht 2-16P @ 29\mod_140532 1505934_68.docx @ 196917 @ 2 @ 1


4.1 Dimensions and weight

Pos: 77 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 78 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/040 Lagerung und Transport/040-0020 Lagerbedingu ngen @ 27\mod_1405319161442_68.d ocx @ 192367 @ 2 @ 1


4.2 Storage conditions

Pos: 79 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 80 /100 Sigma/110 BA Zentrifuge n Sigma (Projekte)/2-16P/040 Lagerung u nd Transport/040-0030 Transporthin weise 2-16P @ 29\mod_140532150657 4_68.docx @ 196931 @ 2 @ 1


The centrifuge can be stored in its original packaging for up to a year.

• Store the centrifuge only in dry rooms.

• The permissible storage temperature is between -20°C and +60°C.

• If you would like to store it for more than one year, or if you intend to

ship it overseas, please contact the manufacturer.

4.3 Notes on transport

• Install the transport safety device (see chapter 4.5 - "Transport safety
device")

• Always lift the centrifuge with a lifting device or with a sufficient number
of people helping you.

• When lifting the centrifuge, always reach under the centrifuge from the

Pos: 81 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

side.

• For transport use suitable packaging and, if at all possible, the original
packaging (see chapter 4.4 - "Packaging").

Laboratory Centrifuge Sigma 2-16P

4 Storage and transport

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

25 / 72

Translation from the original operating manual, part no. 0700602

CAUTION

The centrifuge weighs approx. 32 kg!

CAUTION

The transport safety device must be removed prior to start-up!

Pos: 82 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/040 Lagerung u nd Transport/040-0040 Verpackung 2-16P @ 29\mod_1405321507412_68.d ocx @ 196945 @ 2 @ 1

4.4 Packaging

The centrifuge is packaged in a threepart slip-lid box.

• Take off the lid.

• Remove the box containing the accessories and the packaging material.

• Remove the ring of the slip-lid box.

• Lift the centrifuge upwards with a lifting device or with a sufficient

number of people to lift it safely. When lifting the centrifuge, always

Pos: 83 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 84 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/040 Lagerung u nd Transport/040-0050 Transportsic herung 2-16P - Überschrift @ 29\mod_14 05321508222_68.docx @ 196959 @ 2 @ 1

4.5 Transport safety device

Pos: 85 /100 Sigma/110 BA Zentrifugen Sigma (Projekte)/2-16P/040 Lagerung u nd Transport/040-0051 Transportsic herung 2-16P - Text @ 36\mod_1434605323998 _68.docx @ 263872 @ @ 1

reach under the centrifuge from the side.

• Retain the packaging for any possible future transport of the centrifuge.

The transport safety device consists of a foamed plastic piece in the rotor
chamber.

Removal

• Open the lid by pressing the lid key. If the centrifuge is not connected to
the power supply, use the emergency release of the lid (see chapter

7.1.1 - "Emergency lid release").

• Unscrew the rotor tie-down screw.

• Remove the foamed plastic piece from the rotor chamber, by lifting it

carefully on one side.

• Retain the transport safety device for the possibility of the return of the

Pos: 86 /010 Universalmodule/Abschnitts wechsel @ 0\mod_1202124514062_0.d ocx @ 418 @ @ 1

Pos: 87 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

centrifuge.

Laboratory Centrifuge Sigma 2-16P

5 Set-up and connection

26 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

DANGER

The operating voltage on the name plate must correspond to the local
supply voltage!

Pos: 88 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/050 Aufstellung und Anschluss/050 Aufstellung und Ansc hluss================================== @ 27\mod_14053 19173071_68.docx @ 192381 @ 1 @ 1


5 Set-up and connection

Pos: 89 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/050 Aufstellung und Anschluss/050-0010 Aufstell ort (außer 8KS) @ 27\mod_1405319174 276_68.docx @ 192395 @ 2 @ 1


5.1 Installation site

Pos: 90 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 91 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/050 Aufstellung und Anschluss/050-0020 Energievers orgung------------------------------------------------------ @ 27\mod_1405319175630_68.docx @ 192409 @ 2 @ 1


Operate the centrifuge only in closed and dry rooms.
All the energy supplied to the centrifuge is converted into heat and emitted

to the ambient air.

• Ensure sufficient ventilation.

• Keep a safety distance of at least 30 cm around the centrifuge so that

the vents in the centrifuge remain fully effective.

• Do not subject the centrifuge to thermal stress, e.g. by positioning it
near heat generators.

• Avoid direct sunlight (UV radiation).

• The table must be stable and have a solid, even surface.

• Attention: During transport from cold to warmer places, condensational

water will collect inside the centrifuge. It is important to allow sufficient
time for drying (min. 24 h) before the centrifuge can be used again.

5.2 Power supply

Pos: 92 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/050 Aufstellung und Anschluss/050-0020-0010 Ansc hlussart (Kaltgerätestecker) @ 32\m od_1417599670621_68.docx @ 222346 @ 3 @ 1

5.2.1 Type of connection

Pos: 93 /010 Universalmodule/ Leerzeile @ 0\ mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 94 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/050 Aufstellung und Anschluss/050-0020-0020 Sich erungen bauseits (außer 3-30KS) @ 7\mod_1306312302397_68.docx @ 43513 @ 3 @ 1


5.2.2 Customer-provided fuses

Pos: 95 /010 Universalmodule/Abschnitts wechsel @ 0\mod_1202124514062_0.d ocx @ 418 @ @ 1

Pos: 96 /010 Universalmodule/Seitenwec hsel @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

Sigma centrifuges are units of protection class I. The centrifuges of this
model series have a three-wire power cord with an IEC C13 connector.

Typically, the centrifuge must be protected with 16 Amp L or B fuses that
are to be provided by the customer.

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

27 / 72

Translation from the original operating manual, part no. 0700602

DANGER

• Before the initial start-up, please ensure that your centrifuge is properly
set up and installed (see chapter 5 - "Set-up and connection").

WARNING

Do not place your fingers between the lid and the housing when closing
the lid. Risk of crushing!

NOTE

A flashing lid key indicates that only one lid lock has locked.

Pos: 97 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/062 Betrieb_S pincontrol Easy+Universal/062 Betrieb==== =================== ================== @ 27\mod_140532041 7154_68.docx @ 194019 @ 1 @ 1


6 Using the centrifuge

Pos: 98 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/062 Betrieb_S pincontrol Easy+Universal/062-0010 Erste Inbetriebnahme @ 27\mod_140 5320417875_68.docx @ 194033 @ 2 @ 1


6.1 Initial start-up

Pos: 99 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 11 4 @ @ 1

Pos: 100 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/062-002 0 Einschalten @ 27\mod_14053204186 52_68.docx @ 194047 @ 2 @ 1


6.2 Switching the centrifuge on

• Press the mains power switch.

Pos: 101 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 102 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/062-002 0-0010 Öffnen und Schließen des Deckels - 2 Schlösser @ 27\mod_1405320419 421_68.docx @ 194061 @ 3 @ 1


6.2.1 Opening and closing the lid

The display then illuminates. The centrifuge is ready for operation.

The lid can be opened if the centrifuge is at a standstill and if the lid key is
illuminated.

• Press the lid key in order to open the lid.
The centrifuge cannot be started if the lid is opened.

• To close, press with both hands slightly on the lid until both electrical
locks are locked.

Pos: 103 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

28 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

WARNING

Once a day or after 20 cycles, the rotor tie-down screw must be loosened
by some turns, and the rotor must be lifted and fastened again. This
ensures a proper connection between the rotor and the motor shaft.

CAUTION

The lid screw serves for the fastening of the lid onto the rotor only, not for
the fastening of the rotor onto the motor shaft.

CAUTION

When using rotors for microtiter plate formats:
Ensure that the plate holders are inserted together with the plates into the

buckets.

NOTE

The lid screw serves for the fastening of the lid onto the rotor only, not for
the fastening of the rotor onto the collet chuck.

Pos: 104 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/062-002 0-0020 Einsetzen von Rotoren und Z ubehör -------------------------------- @ 27\mod_1405320420206_68.docx @ 194075 @ 3 @ 1


6.2.2 Installation of rotors and accessories

Pos: 105 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/062 Betrieb/062-0 020-0020-0010 Einsetzen des Rotors 2-1 6P @ 29\mod_1405321530898_68.d ocx @ 197001 @ 4 @ 1


6.2.2.1 Installation of the rotor

• Open the centrifuge lid by pressing the lid key.

• Unscrew the rotor tie-down screw from the motor shaft (counter-

clockwise).

• Lower the rotor with its central bore straight down onto the motor shaft.

• Tighten the rotor tie-down screw clockwise with the supplied rotor

wrench with 5 Nm. In doing so, hold the rotor at its outer rim.

• Prior to installing the lid, check for the tight fit (5 Nm) of the rotor tie­down screw.

• Follow the safety instructions and hazard warnings (see chapter 3 -

Pos: 106 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

"Safety")!

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

29 / 72

Translation from the original operating manual, part no. 0700602

1 Rotor tie down screw
2 Lid
3 Rotor

Pos: 107 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/062 Betrieb/0 62-0020-0020-0020 Einsetzen von Wi nkelrotoren mit hermetisch verschließb arem Deckel 2-16P @ 29\mod_1405321 531516_68.docx @ 197015 @ 4 @ 1


6.2.2.2 Installation of angle rotors with a hermetically sealed lid

• Screw the rotor cover onto the rotor and tighten it.

• Lower the rotor with the cover onto the motor shaft .

• Insert the rotor tie-down screw into the motor shaft. Tighten the rotor tie-

down screw with 5 Nm using the supplied rotor wrench so that the
spring washer assembly is compressed tightly.

• The rotor can also be used without a cover.

• The rotors can be installed or removed with a closed lid after loosening

the rotor tie-down screw.

• Follow the safety instructions and hazard warnings (see chapter 3 ­"Safety")!

Pos: 108 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Fig. 5: Angle rotor with a hermetically sealed lid

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

30 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

CAUTION

Please consider the maximum permissible gravitational field of 12,000 x g.
An excess gravitational field will result in an increased risk of glass
breakage!

Pos: 109 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/062 Betrieb/0 62-0020-0020-0021 Einsetzen des Mi krohämatokrittellers 2-16P @ 29\mod _1405321532319_68.docx @ 197029 @ 4 @ 1

6.2.2.3 Installation of the microhaematocrit rotor

• Open the centrifuge lid by pressing the lid key.

• Replace the rotor tie-down screw with the rotor tie-down screw of the

microhaematocrit rotor (part no. 81543, included in the scope of supply
of the microhaematocrit rotor), and tighten it loosely.

• Lower the rotor with its central bore straight down onto the motor shaft.

• Tighten the rotor tie-down screw clockwise with 5 Nm using the supplied

rotor wrench. In doing so, hold the microhaematocrit rotor with one hand
and tilt it slightly in order to prevent the motor shaft from slipping
through.

• Check the rotor for a correct and tight fit.

• Follow the safety instructions and hazard warnings (see chapter 3 -

"Safety")!

Operation

• Fill the capillary tubes with blood and seal them at one end with putty or
by fusion.

• Place the capillary tubes into the recesses of the rotor with the sealed
end against the rubber ring. Ensure that the capillary tubes fit tightly
against the rubber ring. The opposite places must be loaded.

• Screw on the rotor cover.

• Close the centrifuge lid.

• Enter the following parameters: RCF 12,000 x g, runtime approx.

5 minutes.

• Start the centrifuge.

• Open the centrifuge lid when the rotor has stopped.

• Unlock the rotor cover and lift it off.

Evaluation

• Put the reader onto the microhaematocrit rotor.

• Turn the reader and perform a fine adjustment with the aid of the central

eccentric mechanism in order to localize the O-point and the maximum
liquid point in the capillary tubes. You can now read the percentage
value.

• Remove the capillary tubes. Some of the tubes can also be evaluated
with the reader outside of the rotor (see the instructions for use on the

Pos: 110 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

back of the reader).

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

31 / 72

Translation from the original operating manual, part no. 0700602

correct

incorrect

correct

incorrect

Pos: 111 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/060 Betrieb_ Spincontrol L+S/060-0020-0020-0030 Einsetzen von Zubehör 4plätzig bis 2-1 6P bis 3-18KS @ 27\mod_1405319205 710_68.docx @ 192507 @ 4 @ 1


6.2.2.4 Installation of accessories

• Only use inserts that are suitable for the rotor (see chapter 11.1 ­"Range of accessories").

• All buckets of the swing-out roto need to be installed when spinning.

• Always load the axial symmetrical inserts/buckets of the rotors with the

same accessories and fill to avoid imbalance.

Centrifugation with different tube sizes

Working with different tube sizes is possible. In this case, however, it is
very important that axial symmetrical inserts are identical.

Fig. 6: Permissible and impermissible loading of a swing-out rotor with different tube sizes
(example illustrations)

Centrifugation with low capacity

• Install the tubes axial symmetrically so that the buckets and their inserts
are loaded evenly.

• It is not permissible to load angle rotors on only one axis.

Fig. 7: Permissible and impermissible loading of an angle rotor and a swing-out rotor

Pos: 112 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

(example illustrations)

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

32 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Pos: 113 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/062-002 0-0020-0040 Adapter @ 28\mod_140 5320421969_68.docx @ 194103 @ 4 @ 1


6.2.2.5 Adapters

In order to ensure easy handling, even if vessels of various sizes are used,
carrier systems were developed.

• Load the opposite adapters with the same number of vessels and with
the same weights in order to avoid imbalance.

• If all of the compartments of a carrier are not used, the buckets must be

Pos: 114 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 115 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/062-002 0-0020-0050 Gefäße @ 28\mod_1405 320422786_68.docx @ 194117 @ 4 @ 1


loaded evenly. Loading the edges of a bucket only is not permissible.

6.2.2.6 Tubes

• Load the tubes outside of the centrifuge. Liquids in the buckets or
multiple carriers cause corrosion .

• Fill the tubes carefully and arrange them according to their weight.
Imbalances result in the excessive wear of the bearings.

• In high-speed angle rotors, the vessels must be filled up to their useful
volume (= the volume stated for the vessel). If the vessels are only
partially filled, they will deform. This may result in leaks at the seals that
may become loose.

• When using glass tubes, the maximum value of 4,000 x g must not be
exceeded (except special high-strength glass tubes; please refer to the
information provided by the manufacturer).

• Follow the safety instructions and hazard warnings (see chapter 3 -

Pos: 116 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

"Safety")!

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

33 / 72

Translation from the original operating manual, part no. 0700602

1 Start key
2 Left function knob
3 Display
4 Right function knob
5 Stop key
6 Lid key

1 "Lock" symbol for

lockdown
2 Time field
 Speed field
4 Several display fields

(e.g. for rotor,

deceleration curve or

program selection)
5 RCF field

Pos: 117 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb _Spincontrol Easy+Universal/Spincontrol Universal/062-0030 Spincontrol Uni versal Steuerung------------------- --------------------------- @ 28\mod_140532052516 1_68.docx @ 194411 @ 2 @ 1


6.3 Control system "Spincontrol Universal"

Pos: 118 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0010 Bedienob erfläche Universal @ 28\mod_1405320 525894_68.docx @ 194425 @ 3 @ 1


6.3.1 User interface

The centrifuge is operated via three buttons with integrated light-emitting
diodes and two functions knobs. The display is divided into several different
fields. The various functions of the system can be called up by pressing
and turning the functions knobs.

Fig. 8: User interface of the “Spincontrol Universal” control system

Display

The centrifuge display has the following display fields:

Pos: 119 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Fig. 9: Display of the “Spincontrol Universal” control system

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

34 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

NOTE

If the runtime is changed during an active centrifugation run, the time that
has already elapsed will not be taken into consideration. The centrifuge will
perform a complete run with the new runtime.

Pos: 120 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020 Manueller Betrieb @ 28\mod_1405320526938_68.doc x @ 194439 @ 3 @ 1


6.3.2 Manual mode

Pos: 121 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0010 Start en einer Zentrifugation @ 28\mod_14053205 27735_68.docx @ 194453 @ 4 @ 1


6.3.2.1 Starting a centrifugation run

Pos: 122 /010 Universalmodule/ Leerzeile 8pt @ 12\mod_1337063062114_0.docx @ 65687 @ @ 1

Pos: 123 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Universal/062-0030-0020-0020 Unterbr echen einer Zentrifugation @ 28\ mod_1405320528561_68.docx @ 194467 @ 4 @ 1


6.3.2.2 Interrupting a centrifugation run

Pos: 124 /010 Universalmodule/ Leerzeile 8pt @ 12\mod_1337063062114_0.docx @ 65687 @ @ 1

Pos: 125 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Universal/062-0030-0020-0030 Unterbr echen eines Bremsvorgangs @ 28\m od_1405320529412_68.docx @ 1944 81 @ 4 @ 1


6.3.2.3 Interrupting a deceleration process

Pos: 126 /010 Universalmodule/ Leerzeile 8pt @ 12\mod_1337063062114_0.docx @ 65687 @ @ 1

Pos: 127 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0040 A uswahl, Anzeige und Änderung von Date n @ 28\mod_1405320530250_68.docx @ 194495 @ 4 @ 1


6.3.2.4 Selection, display, and modification of data

Pos: 128 /010 Universalmodule/ Leerzeil e 8pt @ 12\mod_1337063062114_0.docx @ 65687 @ @ 1

Pos: 129 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0050 Lau fzeit - ohne Temp @ 28\mod_1405320 531525_68.docx @ 194509 @ 4 @ 1


6.3.2.5 Runtime

The centrifuge is ready for operation when the start key is illuminated.

• Press the start key in order to start a centrifugation run.

• Press the stop key in order to interrupt a centrifugation run.

The centrifugation run will be terminated prematurely.

• Press the start key during a deceleration process in order to interrupt it
and to restart the centrifuge.

• Turn the left function knob in order to select a field. The selected field
will be preceded by the indication "set", "run", or "save".

• Press the left function knob. The indication ("set", "run", or "save")
flashes and the modification mode is active.

• Turn the right function knob in order to modify the set value of the
selected field.

• Press the fright unction knob again to confirm the input and to quit the
modification mode

• If the input is not confirmed, the value will be automatically reset to the
last setting.

The runtime is displayed in the left section of the display and can be set at
different intervals in a range from 10 seconds to 11 hours and 59 minutes.

The parameter runtime can be changed during the centrifugation.

Fig. 10: Setting the runtime

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

35 / 72

Translation from the original operating manual, part no. 0700602

Continuous run

During the continuous run, the runtime of the centrifuge is unlimited and
must be stopped manually. The centrifuge accelerates during the
continuous run until the set speed is reached.

• Select the modification mode and turn the function knob from the time

00:10 anti-clockwise or from the time 11:59 clockwise. The indication
"Cont" and the symbol "∞" will be displayed in the time field.

Fig. 11: Indication “Cont” during a continuous run

Short run

• Keep the start key pressed during the short run.

During the short run, the centrifuge accelerates at maximum power until the
maximum speed is reached. The runtime is counted and in the time field
the indication "short run" is displayed.

When the start key is released, the centrifuge decelerates at maximum
power to a standstill.

Fig. 12: Indication “short run” during a short run

Changing the time increments

By default, the runtime is changed in intervals of 10 sec (in the mode
min:sec) or in intervals of 10 min (in the mode hrs:min)

To change the runtime in intervals of 1 sec or 1 min:

• Keep the stop key pressed while setting the desired runtime with the

Pos: 130 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

right function knob

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

36 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Pos: 131 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0060 Dreh zahl - ohne Temp @ 28\mod_1405320 532488_68.docx @ 194523 @ 4 @ 1


6.3.2.6 Speed

The set speed of the centrifuge is displayed in the upper area of the speed
field. The actual value is displayed right below. The speed is stated as the
number of revolutions per minute (rpm). The speed/RCF values are
interdependent (see chapter 2.2.2.1 - "Speed, radius, and relative
centrifugal force"). The maximum speed values depend on the rotor that is
used.

It is also possible to change the RCF value during a centrifugation run.

Fig. 13: Setting the speed

Changing the speed increments

By default, the speed is changed in intervals of 100 rpm.
To change the speed in intervals of 1 rpm:

• Keep the stop key pressed while setting the desired speed with the right

Pos: 132 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 133 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0070 RZB - ohne Temp @ 28\mod_14053205333 74_68.docx @ 194537 @ 4 @ 1


function knob.

6.3.2.7 Relative centrifugal force (RCF)

The set RCF value of the centrifuge is displayed in the upper area of the
RCF field. The actual value is displayed right below. The RCF value is
stated as a multiple of the gravitational acceleration (x g). The values of
speed and RCF are interdependent (see chapter 2.2.2.1 - "Speed, radius,
and relative centrifugal force"). The maximum speed/RCF values depend
on the rotor that is used.

The parameter RCF can be changed during the centrifugation.

Pos: 128 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 12 @ @ 1
Pos: 134 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Abb. 14: Setting the RCF-value

Changing the RCF increments

By default, the RCF value is changed in intervals of 10 x g.
To change the RCF value in intervals of 1 x g:

• Keep the stop key pressed while setting the desired RCF value with the
right function knob.

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

37 / 72

Translation from the original operating manual, part no. 0700602

NOTE

The rotor selection can only be changed when the centrifuge is at a
standstill.

NOTE

Attention – special case!!!

If the rotors 12072 or 12107 are not preselected, rotor 12073 will be
automatically displayed for rotor 12072, and rotor 12133 for rotor 12107.

This setting does not cause any problems for the centrifugation since the
suggested rotors have the same maximum speed.

Pos: 135 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0080 Rotora uswahl - ohne Temp @ 28\mod_1405 320534301_68.docx @ 194551 @ 4 @ 1


6.3.2.8 Rotor selection

This field shows the rotor that is currently being used.
The centrifuge is equipped with an automatic rotor identification system.

After the installation of the rotor, the rotor ID will be displayed.
It is also possible to manually select the rotor.

• Select the "Rotor" field and confirm the selection. The indication "set"

• Turn the right function knob until the desired rotor number is selected.

• Press the right function knob to confirm the input. The selected rotor will

flashes in the activated status.

be adopted.

Fig. 15: Rotor selection

Pos: 136 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 137 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Universal/062-0030-0020-0090 S oftstart- und Softstop-Funktionen @ 28\ mod_1405320535209_68.docx @ 194565 @ 4 @ 1

6.3.2.9 Softstart and softstop function

The softstart function is used to extend the acceleration time, whereas the
softstop function is used to extend the deceleration time. The current
combination is shown on the display.

• Press the stop key repeatedly until the desired combination is
displayed. The possible combinations are called cyclically.

– If "soft start" is displayed, only the softstart function is activated.
– If "soft start" and "soft stop" are displayed, the softstart and the

softstop function are activated.

– If "soft stop" is displayed, only the softstop function is activated.
– If "soft start" is displayed and the "soft stop" indication flashes, the

softstart function and the brakeless deceleration are activated.

– If the "soft stop" indication flashes, the brakeless deceleration is

activated.

– If no indication is displayed, the standard operating mode is

Pos: 138 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

restored.

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

38 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Pos: 139 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0100 Automatische D eckelöffnung - ohne Temp @ 28\mod _1405320536098_68.docx @ 194579 @ 4 @ 1


6.3.2.10 Automatic lid opening function ("Auto-Lid-Open")

The automatic lid opening function must be activated so that the lid opens
automatically at the end of the operation.

In order to activate the automatic lid opening function:

• Press the lid key three times and on the third time hold it down for
approx. two seconds.

• Proceed in the same way in order to deactivate the function.

After every change, the current status of the setting is displayed in the form

Pos: 140 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 141 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0110 A kustisches Signal - ohne Temp @ 28 \mod_1405320536975_68.docx @ 1945 93 @ 4 @ 1

of a message running over the display ("Auto-Lid-Open on" or "off").

6.3.2.11 Sound signal ("Buzzer")

This function is used to set an acoustic signal that sounds at the end of the
centrifugation run and also in the event of an imbalance or error message.

In order to activate the sound signal:

• Press the lid key five times and on the fifth time hold it down for approx.
two seconds.

• Proceed in the same way in order to deactivate the function..

After every change, the current status of the setting is displayed in the form

Pos: 142 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 143 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0020-0141 Eing abesperre - ohne Temp @ 28\mod_14 05320537860_68.docx @ 194607 @ 4 @ 1

of a message running in the display ("Buzzer on" or "off").

6.3.2.12 Input lock

In order to prevent the centrifuge from being manipulated by unauthorised
persons, inputs can be disabled via the menu. Inputs via the keypad, i.e. for
starting or stopping a centrifugation run or for opening the lid, are enabled.

Activating a simple input lock:

• Turn the left function knob until the symbol " " is displayed in the
upper left corner of the display. The centrifuge can only be started and
stopped, but the parameters cannot be changed.

As long as the symbol is displayed, the centrifuge can only be started and
stopped, but the parameters cannot be changed.

Activating a permanent input lock:

• Press the start key three times and hold for approximately 2 seconds
when pressing it for the third time.

After the activation of this function, the padlock symbol flashes. The input
lock is activated.

• Proceed in the same manner in order to deactivate the permanent input
lock.

The current status remains active even if the centrifuge is switched off with
the help of the mains power switch.

Pos: 144 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Fig. 16: "Padlock" symbol indicating an activated input lock

Laboratory Centrifuge Sigma 2-16P

6 Using the centrifuge

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

39 / 72

Translation from the original operating manual, part no. 0700602

NOTE

Program numbers that are already occupied will be overwritten with the
current data.

Pos: 145 /100 Sigma/100 BA Zentrifugen Sigma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Universal/062-0030-0030 Programm @ 28\mod_1405320538821_68.doc x @ 194621 @ 3 @ 1

6.3.3 Programs

A program contains all the data that are required for a centrifuge run.
Certain sedimentation results can be repeated under identical conditions. A
maximum of 50 programs can be stored and loaded under the numbers 1 –

Pos: 146 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 147 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0030-0010 A ktuelle Einstellungen speichern @ 28\ mod_1405320539762_68.docx @ 1946 35 @ 4 @ 1

6.3.3.1 Saving the current settings

50, when the centrifuge is at a standstill.

• Select the desired parameters.

• Turn the left function knob until "save" is indicated and confirm the

selection by pressing the right function knob. The indication "save"
flashes and the modification mode is active.

• Select the desired program number by turning the right function knob
and confirm the selection by pressing the right function knob.

Pos: 148 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 149 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/Spincontrol Uni versal/062-0030-0030-0020 Gesp eicherte Programme abrufen @ 28\mod_14053 20540742_68.docx @ 194649 @ 4 @ 1


Fig. 17: Saving a program; here: program no. 08

6.3.3.2 Calling up stored programs

• Turn the left function knob until "run" is indicated and confirm the
selection by pressing the right function knob. The indication "run"
flashes and the modification mode is active.

• Select the desired program number by turning the right function knob
and confirm the selection by pressing the right function knob.

The program is now loaded

Fig. 18: Loading a program; here: program no. 8

Pos: 150 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1
Pos: 151 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/062 Betrieb_ Spincontrol Easy+Universal/062-004 0 Ausschalten @ 28\mod_1405320423 571_68.docx @ 194131 @ 2 @ 1


6.4 Switching the centrifuge off

• Open the centrifuge when it is not in use so moisture can evaporate.

Pos: 152 /010 Universalmodule/Abschnitt swechsel @ 0\mod_1202124514062_0. docx @ 418 @ @ 1

• Switch the centrifuge off by pressing the mains power switch.

Laboratory Centrifuge Sigma 2-16P

7 Malfunctions and error correction

40 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

NOTE

Error messages can be eliminated by pressing the lid key. The error itself
will not be eliminated, but the centrifuge can be operated again.

Type of error

Possible reason

Correction

No indication on the display

No power in the mains supply

Check fuse in the mains supply

Power cord is not plugged in

Plug in power cord correctly

Fuses have tripped

Reactivate temperature fuse (see
chapter 5.2.1 - "Type of connection")

Mains power switch off

Switch mains power switch on

Centrifuge cannot be started:
start key LED is not illuminated

Several

Power off/on. If the error occurs
again, contact service

Centrifuge cannot be started:
lid key LED flashes

The lid lock is not closed correctly

Open and close the lid. If the error
occurs again, contact service

Centrifuge decelerates during
operation

Brief mains power failure

Press start key in order to restart the
centrifuge

System error

Power off/on. If the error occurs
again, contact service

Centrifuge decelerates during
operation, imbalance message is
displayed

– Improper loading
– Centrifuge is inclined
– Drive problem
– Centrifuge was moved during run

Balance load and restart the
centrifuge. If the error occurs again,
contact service(see chapter 7.1.1 ­"Emergency lid release")

– Ungreased load- bearing bolts

Clean and grease load- bearing bolts

Lid cannot be opened

Lid lock has not released

Unlock the lid manually and contact
service

Lid seal sticks

Clean the lid seal and apply talcum
powder

Temperature value cannot be reached
(only for refrigerated centrifuges)

Condenser dirty (only air-cooled units)

Clean the condenser. If the error
occurs again, contact service

Pos: 153 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1
Pos: 154 /100 Sigma/100 BA Zentrifug en Sigma (Standardmodule)/070 Störu ngen und Fehlersuche/070 Störungen un d Fehlersuche============== =============== @ 28\mod_1405320576 072_68.docx @ 194663 @ 1 @ 1


7 Malfunctions and error correction

Pos: 155 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/070 Störungen u nd Fehlersuche/070-0016 Allge meine Störungen_Spincontrol Easy+Uni versal @ 28\mod_1405320582167_68.do cx @ 194747 @ 2 @ 1


7.1 General malfunctions

Error messages are displayed with a code number. If the acoustic signal is
activated, it sounds when the error message is displayed.

• Eliminate the source of the problem (see table below).

• Acknowledge the error messages by pressing the lid key.

Pos: 156 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

7 Malfunctions and error correction

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

41 / 72

Translation from the original operating manual, part no. 0700602

1 String
2 Stopper
3 Screw

WARNING

Do not unlock or open the lid unless the rotor is at a standstill.

Pos: 157 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/070 Störu ngen und Fehlersuche/070-0010-0010 N otentriegelung 2-16P @ 29\mod_14053215421 03_68.docx @ 197043 @ 3 @ 1

7.1.1 Emergency lid release

In the event of a power failure, it is possible to manually open the centrifuge
lid using two stoppers that are secured with screws. They are located on
the front of the bottom panel (see figure).

Fig. 19 : Position of the opening for the emergency release

• Switch off the mains power switch and disconnect the power cord from
the socket

• Unscrew the screws in the stoppers.

• Remove the stoppers (see figure below, item 2) on the front of the

button, e.g. with a screwdriver.

Fig. 20: Screw for the emergency lid release

• The stoppers are connected with a string. Pull the string in order to
release the lid.

• Then, reinsert the stoppers into the openings.

If the lid is opened via the emergency lid release system during a centrifuge

Pos: 158 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

run, the centrifuge will be switched off immediately and decelerate in an
unbraked manner.

Laboratory Centrifuge Sigma 2-16P

7 Malfunctions and error correction

42 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Error no.

Kind of error

Measures

Note

1-9

System error

• Allow to slow down

• Power off/on

All these errors stop the
centrifuge or cause it to
decelerate brakeless

10-19

Speedometer error

• Allow to slow down

• Power off/on

20-29

Motor error

• Power off

• Ensure ventilation

30-39

EEPROM error

• Allow to slow down

• Power off/on

With error 34, 35, and 36, the
centrifuge will stop;
with error 37 and 38 only an
error message will be given

40-45

Temperature error
(only for refrigerated centrifuges)

• Allow to slow down

• Power off

• Allow to cool down

• Provide better ventilation (only

air cooled centrifuges)

• Provide sufficient water
throughput (only water cooled
centrifuges)

46-49

Imbalance error (only for centrifuges
with imbalance monitoring system)

• Allow to slow down

• Power off

• Eliminate the imbalance

50-59

Lid error

• Press lid key

• Close lid

• Remove foreign matter from

the opening of the lid lock
device

With error 50 and 51, the
centrifuge will stop

60-69

Process error

• Allow to slow down

• Power off/on

With error 60 message
"power failure during run",
with error 61, the message
"stop after power on"

70-79

Communication error

• Allow to slow down

• Power off/on

80-89

Parameter error

• Power off

• Allow to cool down

• Provide for better ventilation

With error 83, error message
only

90-99

Other errors

• Check connections

• Provide sufficient water

throughput (only water cooled
centrifuges)

NOTE

If it is impossible to eliminate the errors, contact the service!

Pos: 159 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/070 Störungen u nd Fehlersuche/070-0020 Tabell e der Fehlermeldungen @ 28\mod_14 05320582996_68.docx @ 194761 @ 2 @ 1


7.2 Table of error codes

Pos: 160 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

7 Malfunctions and error correction

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

43 / 72

Translation from the original operating manual, part no. 0700602

NOTE

• If you would like to utilise our service, please state the type of your
centrifuge and its serial number.

Pos: 161 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/070 Störungen u nd Fehlersuche/070-0030 Konta kt im Servicefall @ 28\mod_1405320584 361_68.docx @ 194775 @ 2 @ 1


7.3 Service contact

In the event of queries, malfunctions, or spare part enquiries:

From Germany:

Contact
Sigma Laborzentrifugen GmbH

An der Unteren Söse 50
37520 Osterode (Germany)
Tel. [phone]
E-mail: [email protected]

Outside Germany:

Contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de  [Sales Partners]

Pos: 162 /010 Universalmodule/Abschnitt swechsel @ 0\mod_1202124514062_0. docx @ 418 @ @ 1

Pos: 163 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

44 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

CAUTION

If corrosion or other damage occurs due to improper care, the
manufacturer cannot be held liable or subject to any warranty claims.

WARNING

Take suitable precautions for your own safety if there is a risk of toxic,
radioactive, or pathogenic contamination.

Pos: 164 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080 Wartung und Inst andhaltung========================= @ 28\mod_1405320612731_68.docx @ 194789 @ 1 @ 1


8 Maintenance and service

The centrifuge, rotor, and accessories are subject to high mechanical
stress. Thorough maintenance performed by the user extends the service

Pos: 165 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 166 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010 Wartung sarbeiten--------------------------------------- @ 28\mod_1405320613978_68.docx @ 194803 @ 2 @ 1


life and prevents premature failure.

• Use soap water or other water-soluble, mild cleaning agents with a pH
value between 6 and 8 for cleaning the centrifuge and accessories (see
also chapter 8.2 - "Sterilisation and disinfection of the rotor chamber
and accessories").

• Avoid corrosive and aggressive substances.

• Do not use solvents.

• Do not use agents with abrasive particles.

• Do not expose the centrifuge and rotors to intensive UV radiation or

thermal stress (e.g. by heat generators).

8.1 Maintenance

Pos: 167 /100 Sigma/100 BA Zentrifug en Sigma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010-0010 Zentrifuge @ 28\mod_1405320615295_6 8.docx @ 194817 @ 3 @ 1


8.1.1 Centrifuge

Pos: 168 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

• Unplug the mains power plug before cleaning.

• Carefully remove all liquids, including water and particularly all the

solvents, acids, and alkaline solutions from the rotor chamber using a
cloth in order to avoid damage to the motor bearings.

• If the centrifuge has been contaminated with toxic, radioactive, or
pathogenic substances, clean the rotor chamber immediately with a
suitable decontamination agent (depending on the type of
contamination).

• After every cleaning process, grease the motor shaft slightly with a
small amount of heavy-duty grease for load-bearing bolts (part no.

71401) and distribute the grease with a cloth so that it forms a thin
layer.

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

45 / 72

Translation from the original operating manual, part no. 0700602

CAUTION

For the care of the accessories, special safety measures must be
considered as these are measures that will ensure operational safety at
the same time!

CAUTION

Do not clean the accessories in a dishwasher!

Cleaning in a dishwasher removes the anodised coating; the result is
cracking in areas that are subject to stress.

WARNING

Plastic accessories must not be greased!

Pos: 169 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010-0020 Zubehör (außer 8K) @ 28\mod_140532 0618247_68.docx @ 194845 @ 3 @ 1


8.1.2 Accessories

• Immediately rinse off the rotor, buckets, or accessories under running
water if they have come into contact with any liquids that may cause
corrosion. Use a brush for test tubes in order to clean the bores of angle
rotors. Turn the rotor upside down and allow it to dry completely.

• Clean the accessories outside the centrifuge once a week or preferably
after each use. Adapters should be removed, cleaned and dried.

• If the rotors or accessories have been contaminated with toxic,
radioactive, or pathogenic substances, clean them immediately with a
suitable decontamination agent (depending on the type of
contamination). Take suitable precautions for your own safety if there is
a risk of toxic, radioactive, or pathogenic contamination.

• Dry the accessories with a soft cloth or in a drying chamber at

Pos: 170 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 171 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010-0020-0 010 Kunststoffzubehör @ 28\mod_1405 320619602_68.docx @ 194859 @ 4 @ 1


approx. 50°C.

8.1.2.1 Plastic accessories

The chemical resistance of plastic decreases with rising temperatures (see
chapter 11.4 - "Resistance data").

• If solvents, acids, or alkaline solutions have been used, clean the plastic
accessories thoroughly.

Pos: 172 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

46 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

NOTE

When using plastic buckets, the load-bearing bolts must not be greased!

Pos: 173 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010-0030 Rotor, Becher und Vielfachträger (außer Basic) @ 28\mod_1405320621063_68. docx @ 194873 @ 3 @ 1


8.1.3 Rotors, buckets and carriers

Rotors, buckets and carriers are produced with the highest precision, in
order to withstand the permanent high stress from high gravitational fields.

Chemical reactions as well as stress-corrosion (combination of oscillating
pressure and chemical reaction) can affect or destroy the metals. Barely
detectable cracks on the surface can expand and weaken the material
without any visible signs.

• Check the material regularly (at least once a month) for
– cracks
– visible damage of the surface
– pressure marks
– signs of corrosion
– other changes.

• Check the bores of the rotors and multiple carriers.

• Replace any damaged components immediately for your own safety.

• After every cleaning process, grease the rotor tie-down screw slightly

with a small amount of heavy-duty grease for load-bearing bolts (part
no. 71401) and distribute the grease with a cloth so that it forms a thin

Pos: 174 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 175 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/080 Wartung u nd Instandhaltung/080-0010-0030-0 010 Mikrohämatokritteller 2-16P @ 29\ mod_1405321549785_68.docx @ 197057 @ 3 @ 1


layer.

8.1.4 Microhaematocrit rotor

• Remove the microhaematrocrit rotor for cleaning.

• Wipe the centrifuge chamber clean.

• Replace the rubber ring in the event of wear or glass breakage (part no.

Pos: 176 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 177 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010-0040 Tragbolzen (nur 2-6, 2-6E, 2-16P, 2-16 KL) @ 44\mod_1467889556212_68.doc x @ 436524 @ 3 @ 1


16001 for rotor 11409).

8.1.5 Load bearing bolts

Pos: 178 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Plastic buckets

Aluminium buckets

When using aluminium buckets, only greased load bearing bolts ensure the
even swinging of the buckets and, therefore, the quiet run of the centrifuge.
Non-greased bolts can lead to a system shut-down due to imbalances.

• After every cleaning process, grease the load-bearing bolts of the rotor
slightly with a small amount of heavy-duty grease for load-bearing bolts
(part no. 71401) and distribute the grease with a cloth so that it forms a
thin layer.

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

47 / 72

Translation from the original operating manual, part no. 0700602

CAUTION

In the case of glass breakage, immediately remove all glass particles (e.g.
with a vacuum cleaner). Replace the rubber cushions since even thorough
cleaning will not remove all glass particles.

DANGER

If dangerous materials (e.g. infectious and pathogenic substances) are
used, the centrifuge and accessories must be disinfected.

Pos: 179 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0010-0060 Glasbruch @ 28\mod_1405320623434_ 68.docx @ 194901 @ 3 @ 1


8.1.6 Glass breakage

Glass particles will damage the surface coating (e.g. anodising) of the
buckets, which will then lead to corrosion.

Glass particles in the rubber cushions of the buckets will cause glass
breakage again.

Glass particles on the pivot bearing of the load- bearing bolts prevent the
buckets and carriers from swinging evenly, which will cause an imbalance.

Glass particles in the rotor chamber will cause metal abrasion due to the
strong air circulation. This metal dust will not only pollute the rotor chamber,
rotor, and materials to be centrifuged but also damage the surfaces of the
accessories, rotors, and rotor chamber.

In order to completely remove the glass particles and metal dust from
the rotor chamber:

• Grease the upper third of the rotor chamber with e.g. Vaseline.

• Then, let the rotor rotate for a few minutes at a moderate speed

• Remove the grease with the glass and metal particles with a cloth.

Pos: 180 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 181 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartu ng und Instandhaltung/080-0020 Steril isation und Desinfektion von Rotorkam mer und Zubehör @ 28\mod_140532062 6090_68.docx @ 194929 @ 2 @ 1


• If necessary, repeat this procedure.

(approx. 2000 rpm). The glass and metal particles will now collect at the
greased part.

8.2 Sterilisation and disinfection of the rotor chamber and
accessories

• Use commercially-available disinfectants such as, for example,
Sagrotan, Buraton, or Terralin (available at chemist’s shops or
drugstores).

• The centrifuge and the accessories consist of various materials. A
possible incompatibility must be considered.

• Before using cleaning or decontamination agents that were not
recommended by us, contact the manufacturer to ensure that such a
procedure will not damage the centrifuge.

• For autoclaving, consider the continuous heat resistance of the
individual materials (see chapter 8.2.1 - "Autoclaving").

Please contact us if you have any queries (see chapter 7.3 - "Service

Pos: 182 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

contact").

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

48 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

NOTE

It cannot be excluded that plastic parts, e.g. lids or carriers, may deform
during autoclaving.

Accessories

Max. temp.

(°C)

Min. time

(min)

Max. time

(min)

Max. cycles

Aluminium buckets

134-138

3 5 -

Aluminium rotors

134-138

3 5 -

Glass tubes

134-138

3

40

-

Polyallomer / polycarbonate rectangular carriers

115-118

30

40

-

Polyallomer / polycarbonate round carriers

115-118

30

40

-

Polyamide buckets

115-118

30

40

10

Polycarbonate / polyallomer lids for angle rotors

115-118

30

40

20

Polycarbonate tubes

115-118

30

40

20

Polyphenylsulfone (PPSU) caps for buckets

134-138

3 5 100

Polypropylene balance adapter for blood-bag systems

115-118

30

40

n.s.

Polypropylene copolymer tubes

115-121

30

40

20

Polypropylene rectangular carriers

115-118

30

40

-

Polypropylene rotors

115-118

30

40

20

Polypropylene round carriers

115-118

30

40

-

Polysulfone caps for buckets

134-138

3 5 100

Polysulfone lids for angle rotors

134-138

3 5 100

Rubber adapters

115-118

30

40

-

Stainless-steel balance weight for blood-bag systems

121

30

30

n.s.

Teflon tubes

134-138

3 5 100

Pos: 183 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0020-0010 Autoklavieren @ 28\mod_1405320627 440_68.docx @ 194943 @ 3 @ 1


8.2.1 Autoclaving

The service life of the accessories essentially depends on the frequency of
autoclaving and use.

• Replace the accessories immediately when the parts show changes in
colour or structure or in the occurrence of leaks etc.

• During autoclaving, the caps of the tubes must not be screwed on in
order to avoid the deformation of the tubes.

Pos: 184 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

49 / 72

Translation from the original operating manual, part no. 0700602

DANGER

In the event of service work that requires the removal of the panels, there
is a risk of electric shock or mechanical injury. Only qualified specialist
personnel is authorised to perform this service work.

NOTE

• If you would like to utilise our service, please state the type of your
centrifuge and its serial number.

Pos: 185 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0030 Instan dhaltungsarbeiten @ 28\mod_1405320628810 _68.docx @ 194957 @ 2 @ 1


8.3 Service

The centrifuge is subject to high mechanical stress. In order to be able to
withstand this high level of stress, high-quality components were used
during the production of the centrifuge. Nevertheless, wear cannot be
excluded and it may not be visible from the outside. Especially the rubber
parts that are – among other things – part of the motor suspension, are
subject to ageing.

This is why we recommend having the centrifuge checked by the
manufacturer during an inspection once per year in the operating state and
once every three years in the dismantled state. Rubber parts should be
replaced after three years.

Information and appointments:

In Germany:

Contact
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode (Germany)
Tel. [phone]
E-mail: [email protected]

Outside Germany:

Contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de  [Sales Partners]

Pos: 186 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

50 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

NOTE

We will return the part/unit if no declaration of decontamination is provided!

NOTE

If the form does not include a description of the malfunction, neither a
refund nor a credit note can be issued. In this case, we reserve the right to
return the part/unit to you at your expense.

Pos: 187 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/080 Wartung u nd Instandhaltung/080-0040 Rüc ksendung defekter Teile @ 28\mod_1405 320630226_68.docx @ 194971 @ 2 @ 1


8.4 Return of defective parts

Although we exercise great care during the production of our products, it
may be necessary to return a unit or accessory to the manufacturer.

In order to ensure the quick and economical processing of returns of
centrifuges, spare parts, or accessories, we require complete and extensive
information concerning the process. Please fill in the following forms
completely, sign them, enclose them with the return package, and send
them together with the product to:

Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode (Germany)

1. Declaration of decontamination
As a certified company and due to the legal regulations for the
protection of our employees and of the environment, we are obliged to
certify the harmlessness of all incoming goods. For this purpose, we
require a declaration of decontamination.

• The form must be filled in completely and signed by authorised and

specialised personnel only.

• Affix the original form in a clearly visible manner to the outside of the

packaging.

2. Form for the return of defective parts
This form is for the product-related data. They facilitate the assignment,
and they enable the quick processing of the return. If several parts are
returned together in one packaging, please enclose a separate problem
description for every defective part.

• A detailed problem description is necessary in order to perform the

repair quickly and economically.

• Upon request, we will prepare and submit to you a cost estimate
prior to performing the repair. Please confirm such cost estimate
within 14 days. If the cost estimate has still not been confirmed after
4 weeks, we will return the defective part/unit. Please note that you
must bear the incurred costs.

Laboratory Centrifuge Sigma 2-16P

8 Maintenance and service

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

51 / 72

Translation from the original operating manual, part no. 0700602

NOTE

The defective part/unit must be packaged in a transport-safe manner.
Please use the original packaging for the unit, if at all possible.

If the product is dispatched to us in unsuitable packaging, you will be
charged the cost for returning it to you in new packaging.

The forms can be downloaded online from

Pos: 188 /010 Universalmodule/Abschnitt swechsel @ 0\mod_1202124514062_0. docx @ 418 @ @ 1

Pos: 189 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

www.sigma-zentrifugen.de  [Service]  [Overhaul and repair].

Laboratory Centrifuge Sigma 2-16P

9 Disposal

52 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

In accordance with the directive 2002/96/EC, SIGMA centrifuges are
marked with the symbol shown to the left. This symbol means that it is not
permissible to dispose of the unit among household waste.

• You can return these centrifuges free of cost to
Sigma Laborzentrifugen GmbH.

• Ensure that the unit is decontaminated. Fill in a declaration of
decontamination (see chapter 8.4 - "Return of defective parts").

• Comply with any other applicable local rules and regulations.

Pos: 190 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/090 Entsorgung/ 090 Entsorgung======== ============================= @ 28\ mod_1405320658842_68.docx @ 194985 @ 1 @ 1


9 Disposal

Pos: 191 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/090 Entsorgung/ 090-0010 Entsorgung der Zentrif uge---------------------------------------------- @ 28\mod_1405320660029_68.docx @ 194999 @ 2 @ 1


9.1 Disposal of the centrifuge

Pos: 192 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 193 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/090 Entsorgung/ 090-0020 Entsorgung der Verp ackung-------------------------------------------------- @ 28\mod_1405320661383_68.docx @ 195013 @ 2 @ 1


9.2 Disposal of the packaging

• Use the packaging to return the centrifuge for disposal or

• dispose of the packaging, after having separated the individual

materials.

Pos: 194 /010 Universalmodule/Abschnitt swechsel @ 0\mod_1202124514062_0. docx @ 418 @ @ 1

Pos: 195 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

• Comply with all local rules and regulations.

Laboratory Centrifuge Sigma 2-16P

10 Technical data

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

53 / 72

Translation from the original operating manual, part no. 0700602

Manufacturer

Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode (Germany)

Type:

2-16P

Connection requirements
Electr. connection:

Protection class:
IP code:

see name plate
I
20

Connected load (kVA):
Power consumption (kW):
Max. current consumption (A):

Input fuse (AT):

0.30

0.34

1.4 (at 220-240 V / 50 Hz)

2.8 (at 120 V / 60 Hz)

4.0 (at 220-240 V / 50 Hz)

Performance data
Max. speed (rpm):

Max. capacity (ml):
Max. gravitational field (x g):
Max. kin. energy (Nm):

15,000
408
20,627
7,756

Other parameters
Time range:

Storage locations:

10 sec – 11h 59 min, short run, continuous run
50

Physical data
Height (mm):

Height with open lid (mm):
Width (mm):
Depth (mm):
Weight (kg):

300
610
365
452
32

Noise level (dB(A)):

< 67 (at maximum speed)

NOTE

At a nominal voltage of 100V or 200V, a tolerance of +10% / -5% applies.

Pos: 196 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/100 Technisc he Daten/100 Technische Daten 2-16P==== ===================== ===== @ 29\mod_1405321556174_68.docx @ 197071 @ 1 @ 1

10 Technical data

Pos: 197 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 198 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 199 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/100 Technisch e Daten/100-0010 Umgebungsbedi ngungen ungekühlt @ 28\mod_14053 20680121_68.docx @ 195041 @ 2 @ 1

10.1 Ambient conditions

• The figures are valid for an ambient temperature of +23°C and a

Pos: 200 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

nominal voltage  10 %.

• For indoor use only.

• Allowable ambient temperature +5°C to +40°C.

• Max. relative humidity of air 80% up to 31°C with a linear decrease to

50% relative humidity of air at 40°C.

• Maximum altitude 2,000 m above sea level.

Laboratory Centrifuge Sigma 2-16P

10 Technical data

54 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Pos: 201 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/100 Technisch e Daten/100-0020 Technische Doku mentation @ 28\mod_1405320681369_ 68.docx @ 195055 @ 2 @ 1


10.2 Technical documentation

For environmental reasons, the comprehensive technical documentation of
the centrifuge (e.g. circuit diagrams) and the safety data sheets of the
manufacturers of refrigerant and lubricants are not attached to this
documentation.

Pos: 202 /010 Universalmodule/Abschnitt swechsel @ 0\mod_1202124514062_0. docx @ 418 @ @ 1

Pos: 203 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

You can order these documents from our service department.

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

55 / 72

Translation from the original operating manual, part no. 0700602

Part no.

Description

Max. speed
(rpm)

Max.
gravitational
field (x g)

11122

Swing-out rotor for microtiter plates, incl. 1 set carriers no. 13222,
max. height of plates 56 mm, max. load per bucket approx. 335 gram
Radius max. 12.3 cm, radius min. 4.8 cm

3 000

1 238

11123

Swing-out rotor for microtiter plates, incl. 1 set carriers no. 13223,
max. height of plates 50 mm, max. load per bucket approx. 345 gram
Radius max. 11.9 cm, Radius min. 4.8 cm

4 000

2 111

11124

Swing-out rotor 24 x 1.5 / 2.0 ml, incl. 3 sets buckets no. 13124, for e.g.
reaction vials no. 15008, 15040, incl. hermetic aluminium lid no. 17878
Radius max. 7.4 cm, radius min. 3.5 cm

12 500

12 927

11170

Swing-out rotor, 4 place, for bucket no. 13299, suitable for software
version 27 or higher
Radius max. 14.2 cm, radius min. 3.8 cm

4 500

3 215

11190

Swing-out rotor 4 x 100 ml complete, no. 11192, incl. 2 sets bucket no.
13097 and 4 glass tubes 100 ml no. 15100
Radius max. 13.5 cm, radius min. 5.0 cm

4 500

3 056

11191

Swing-out rotor 16 x 15 ml complete, no. 11192, incl. 2 sets carriers no.
13012 and 16 PS-tubes 15 ml no. 15020
Radius max. 13.4 cm, radius min. 5.1 cm

4 500

3 034

11192

Swing-out rotor, 4 place, for buckets no. 13009, 13012, 13041, 13042,
13097, 13150, 13152, buckets 13041 and 13152 for rotors from the year
2003 onwards only

4 500

11409

Microhematocritrotor suitable for 24 capillary tubes Ø 1.5 x 75 mm, 50 µl
no. 15001, incl. reader no. 17029, usable with reader 17002, incl. rotor
screw 81543
Radius max. 9.0 cm, radius min. 1.5 cm

12 000

14 489

12071

Angle rotor for 12 x 15 ml culture tubes, e.g. no. 15115 and 6 x 50 ml
culture tubes, e.g no. 15151, angle 35°,
Radius max. 12.6 cm, radius min. 5.4 cm

4 000

2 254

12072

Angle rotor 20 x 15 ml complete, incl. 10 sets buckets no. 13011, for tubes
Ø 17 x up to 120 mm, e.g. no. 15015, 15020, 15023, 15024, Monovettes
and culture tubes 15 ml no. 15115
Radius max. 13.9 cm, radius min. 8.0 cm, angle 33°

4 000

2 486

12073

Angle rotor 30 x 15 ml complete, incl. 15 sets buckets no. 13011, for tubes
Ø 17 x up to 120 mm, e.g. no. 15015, 15020, 15023, 15024, Monovettes
and culture tubes 15 ml no. 15115, angle 33°
Radius max. 13.9 cm, radius min. 5.8 cm

4 000

2 486

12133

Angle rotor 30 x 1.5/2.0 ml, for e.g. reaction vials no. 15008, 15040, incl.
hermetic aluminium lid no. 17863
Radius max. 10.0 cm, radius min. 6.7 cm, angle 45°

12 000

16 099

12139

Angle rotor 6 x 30 ml, for tubes no. 15029, 15030, 15032,
incl. hermetic aluminium lid no. 17851
Radius max. 7.8 cm, radius min. 2.1 cm, angle 29°

Attention! Rotors from batch number 201/00 may be run with lid, rotors of
older batches (e.g. 15/00 or ../99) have to be run without lid.

13 500

15 893

12141

Angle rotor 10 x 10 ml, for tubes no. 15000, 15010, 15039,
incl. hermetic aluminium lid no. 17850
Radius max. 7.6 cm, radius min. 2.8 cm, angle 35°

13 500

15 485

Pos: 204 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/110 Anhang/ 110 Anhang============= ======================== @ 28\mod_140 5320699310_68.docx @ 195069 @ 1 @ 1


11 Appendix

Pos: 205 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/110 Anhang/ 110-0010 Zubehörprogramm 2-16P @ 29\mod_1405321564657_68.docx @ 19 7085 @ 2 @ 1

11.1 Range of accessories

Laboratory Centrifuge Sigma 2-16P

11 Appendix

56 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Part no.

Description

Max. speed
(rpm)

Max.
gravitational
field (x g)

12148

Angle rotor 24 x 1.5/2.0 ml, for e.g. reaction vials no. 15008, 15040, incl.
hermetic aluminium lid no. 17864
Radius max. 8.2 cm, radius min. 4.9 cm, angle 45°

15 000

20 627

12180

Angle rotor 12 x 5 ml, Eppendorf reaction caps, incl. hermetic aluminium
lid no. 17180, suitable for software version 27 or higher
Radius max. 8.0 cm, radius 3.9 cm, angle 40°

14 000

17 530

12181

Angle rotor for 6 x 50 ml culture tubes, e.g no. 15151, incl. hermetic
aluminium lid no. 17931, suitable for software version 26 or higher
Radius max. 9.5 cm, radius 3.0 cm, angle 28°

10 000

10 621

13009

Multiple carrier for 5 tubes 5 ml, round and flat bottom, incl. rubber cushion
no. 16005, max. Ø 12. 8/16.5 x 65 - 85 mm, e.g. no. RIA tube 15060,
vacutainer, hemolyse tubes, 1 set = 2 pcs.
Radius max. 11.3 cm, radius min. 5.6 cm

4 500

2 558

13012

Multiple carrier for 4 tubes 5 - 15 ml, round bottom, incl. rubber cushion no.
16015, max. Ø 17/19 x 90 - 105 mm, e.g no. 15015, 15020, 15023, 15024,
Monovettes 9 - 10 ml, 1 set = 2 pcs.
Radius max. 13.4 cm, radius min. 5.1 cm

4 500

3 034

13041

Round bucket for round carriers Ø 43.5 mm, no. 14028 -14035, incl.
polysulfone screw cap no. 17130, max. tube length 110 mm, 1 set = 2 pcs.
Radius max. 14.0 cm, radius min. 3.0 cm

4 500

3 170

13042

Multiple carrier for 6 tubes 5 to 15 ml, round bottom, max. Ø 17/19 x
80 - 105 mm, e.g no. 15000, 15010, 15015, 15020, 15023, 15024, 15039,
Monovettes 9 - 10 ml, Vacutainer, incl. rubber cushion no. 16020,
1 set = 2 pcs.
Radius max. 13.3 cm, radius min. 4.9 cm

4 500

3 011

13097

Round bucket for 1 tube 100 ml, incl. rubber cushion no. 16051,
max. Ø 45.5 x 95 - 105 mm, e.g. no. 15100, 15102, 15103, 15106 and
adapters no. 17925, 17950, 1 set = 2 pcs.
Radius max. 13.5 cm, radius min. 5.0 cm

4 500

3 056

13150

Bucket incl. polysulfone screw cap no. 17151 for 1 culture tube 50 ml,
e.g. no. 15151, 1 set = 2 pcs.

13152

Multiple carrier for 2 culture tubes 15 ml, e.g. no. 15115, 1 set = 2 pcs.
Radius max. 14.3 cm, radius min. 3.3 cm

13299

Round bucket, sealable with cap no. 17299, for round carriers no. 14299 ­14310, 1 set = 2 pcs.

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

57 / 72

Translation from the original operating manual, part no. 0700602

Part no.

Description

13000

Adapter for reaction vials 0.25/0.4 ml no. 15014, 1 set = 2 pcs., suitable for 11124, 12133, 12148

13002

Adapter for reaction vials 0.5/0.75 ml, Ø 7.9/10 x 28/31 mm, e.g. no. 15005, 1 set = 2 pcs., suitable for
11124, 12133, 12148

13021

Adapter for PCR-tube 0.2 ml, e.g no. 15042, 1 set = 2 pcs., suitable for 11124, 12133, 12148

13059

Adapter for 1 round bottom tube 10 ml, max. Ø 16.2/19 x 75 - 85 mm, e.g. no. 15000, 15010, 15039,
1 set = 2 pcs., suitable for 12139

13060

Adapter for 1 culture tube 15 ml no. 15115, 1 set = 2 pcs.

13079

Bottom adapter for 1 tube 50 ml, e.g. no. 15051, 15052, 1 set = 2 pcs.

Adapters

The complete list of accessories can be downloaded from

Pos: 206 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 207 /010 Universalmodule/ Leerzeile @ 0\mod_1202116244500_0.docx @ 1 14 @ @ 1

Pos: 208 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/110 Anhang/ 110-0010-0010 Höchstdrehzahlen von Gefäßen 2-16P @ 29\mod_14053215 65378_68.docx @ 197099 @ 3 @ 1

www.sigma-zentrifugen.de.

11.1.1 Maximum speed for tubes

Some tubes, such as centrifuge glass tubes, microtubes, culture tubes,
fluoropolymer tubes and especially high-volume tubes can be used in our
rotors, buckets, and adapters at higher speeds than their breaking limit.

• Always fill the tubes up to their useful volume (= the volume that is
stated for the tube).

• When using glass tubes, the maximum value of 4,000 x g must not be
exceeded (except special high-strength glass tubes; please refer to the

Pos: 209 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

information provided by the manufacturer).

Laboratory Centrifuge Sigma 2-16P

11 Appendix

58 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Pos: 210 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/110 Anhang/ 110-0010-0023 Radien der Rotoren ( WR+AR+Mikrohämatokritteller) @ 28\ mod_1405320703631_68.docx @ 19512 5 @ 3 @ 1

11.1.2 Rotor radii

The information in the accessories table concerning the radius refers to the
values of the respective rotor as shown below. The radius calculation is
described in (see chapter 2.2.2.1 - "Speed, radius, and relative centrifugal
force").

Fig. 21: Minimum and maximum radius of an angle rotor

Pos: 211 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Fig. 22: Minimum and maximum radius of an swing-out rotor

Fig. 23: Microhaematocrit rotor

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

59 / 72

Translation from the original operating manual, part no. 0700602

Pos: 212 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/110 Anhang/ 110-0020 Drehzahl-Schwerefeld-Diagr amm @ 28\mod_1405320705642_68.d ocx @ 195153 @ 2 @ 1


11.2 Speed-gravitational-field-diagram

Fig. 24: Speed-gravitational-field-diagram

Pos: 213 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

11 Appendix

60 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Rotor /
bucket

Cycles

Service life

("Exp.Date")

Autoclaving Suitable for centrifuge Remarks

9100 15,000

4-15C, 4K15C, 4-16, 4-16S,
4-16K, 4-16KS, 6-15, 6K15,
6-16, 6-16K

without engraving, only
"spincontrol
professional" and
"spincontrol S"

11026 7 years

1-14, 1-14K

12033 5 years

1-16 Edition, 1-16K Edition

12082 7 years

1-14, 1-14K

12083 7 years

1-14, 1-14K

12084 7 years

1-14, 1-14K

12085 7 years

1-14, 1-14K

12092 5 years 20x

1-14, 1-14K

12093 5 years 20x

1-14, 1-14K

12094 5 years 20x

1-14, 1-14K

12096 5 years 20x

1-14, 1-14K

12097 5 years 20x

1-14, 1-14K

12101 5 years 20x

1-15, 1-15K, 1-15P, 1-15PK

12124 5 years 20x

1-15, 1-15K, 1-15P, 1-15PK

12126 5 years 20x

1-15, 1-15K, 1-15P, 1-15PK

12134 5 years 20x

1-16, 1-16K

12135 5 years 20x

1-16, 1-16K

12137 5 years 20x

1-16, 1-16K

12500 7 years

6-15, 6K15, 6-16, 6-16K

12600 7 years

6-16S, 6-16KS

13218 20,000

4-16, 4-16S, 4-16K, 4-16KS,
6-16, 6-16S, 6-16K, 6-16KS

13296 5 years 10x

2-6, 2-6E, 2-7

13299 5 years 10x

2-6, 2-6E, 2-7,
2-16P, 2-16KL, 2-16KHL

13635 25,000

6-16, 6-16K, 6-16S, 6-16KS

13650 20,000

4-5L, 4-16S, 4-16KS,
4-16KHS, 6-16S, 6-16HS,
6-16KS, 6-16KHS

13845 20,000

8K, 8KS, 8KBS

13850 10,000

8K, 8KS, 8KBS

13860 15,000

8K, 8KS, 8KBS

13864 1,000

8K, 8KS, 8KBS without engraving

13865 1,000

8K, 8KS, 8KBS without engraving

13866 1,000

8K, 8KS, 8KBS without engraving

13867 2,500

8K, 8KS, 8KBS without engraving

If no other data concerning the service life are engraved on the rotor or accessory, rotors and
buckets must be checked by the manufacturer after 10 years. After 50,000 cycles, rotors must be
scrapped for safety reasons.

Rotors and accessories
with a different service life

Pos: 214 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/110 Anhang/ 110-0040 Tabelle: Lebensdauer vo n Rotoren und Zubehör @ 28\mod_1405 320709911_68.docx @ 195195 @ 2 @ 1


11.3 Table of the service life of rotors and accessories

Pos: 215 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

61 / 72

Translation from the original operating manual, part no. 0700602

NOTE

The data refer to resistance at 20°C.

- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, weak

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Acetaldehyde

C2H4O

40

3 2 4 2 3 4 4 - 1 4 1

Acetamide

C2H5NO

saturated

1 1 4 1 1 4 4 - 1 - 1

Acetone

C3H6O

100

1 1 4 1 1 4 4 - 1 4 1

Acrylonitrile

C3H3N

100

1 1 4 3 3 4 4 4 1 4 1

Allyl alcohol

C3H6O

96

1 3 3 2 2 2 2 4 1 1 1

Aluminium chloride

AlCl3

saturated

1 3 2 4 1 - 1 - 1 1 4

Aluminium sulfate

Al2(SO4)3

10

1 1 1 3 1 1 1 1 1 1 1

Ammonium chloride

(NH4)Cl

aqueous

1 1 1 2 1 1 1 1 1 1 3

Ammonium hydroxide

NH3 + H2O

30

1 3 4 1 1 2 1 - 1 - 1

Aniline

C6H7N

100

1 3 4 1 2 4 4 4 1 4 1

Anisole

C7H8O

100

3 4 4 1 4 4 2 - 1 4 1

Antimony trichloride

SbCl3

90

1 4 1 4 1 - 1 - 1 - 4

Benzaldehyde

C7H6O

100

1 3 4 1 1 3 4 4 1 4 1

Benzene

C6H6

100

3 2 4 1 3 4 4 - 1 4 1

Boric acid

H3BO3

aqueous

1 3 1 2 1 - - - 1 1 1

Butyl acrylate

C7H12O2

100

1 2 4 2 3 4 4 4 1 - 1

Butyl alcohol, normal

C4H10O

100

1 1 2 1 1 2 2 4 1 1 1

Calcium chloride

CaCl2

alcoholic

1 4 2 3 1 - - 4 1 1 3

Carbon disulfide

CS2

100

4 3 4 2 4 4 4 4 1 3 1

Carbon tetrachloride
(TETRA)

CCl4

100

4 4 4 2 4 4 4 4 1 3 1

Chlorine

Cl2

100

4 4 4 4 4 4 4 4 1 - 3

Chlorine water

Cl2 x H2O

3 4 4 4 3 - 3 3 1 - 4

Chlorobenzene

C6H5Cl

100

3 4 4 1 3 4 4 4 1 4 1

Chloroform

CHCl3

100

3 3 4 4 3 4 4 4 1 4 3

Pos: 216 /100 Sigma/100 BA Zentrifugen Sig ma (Standardmodule)/110 Anhang/ 110-0050 Beständigkeitstabelle @ 28 \mod_1405320711439_68.docx @ 19 5209 @ 2 @ 1


11.4 Resistance data

Laboratory Centrifuge Sigma 2-16P

11 Appendix

62 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, weak

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Chromic acid

CrO3

10

1 4 2 4 1 4 1 - 1 4 1

Chromic potassium
sulphate

KCr(SO4)2 x
12H2O

saturated

1 2 1 3 1 - 1 - 1 - 3

Citric acid

C6H8O7

10

1 1 1 2 1 1 1 1 1 1 1

Citric acid

C6H8O7

50

1 3 1 2 1 - - - 1 1 1

Copper sulphate

CuSO4 x 5H2O

10

1 1 1 1 1 1 1 1 1 1 4

Cyclohexanol

C6H12O

100

1 1 3 1 1 1 1 4 1 2 1

Decane

C10H22

100

- 1 2 1 3 - - - 1 2 1

Diaminoethane

C2H8N2

100

1 1 3 1 1 - 3 4 1 1 1

Diesel fuel — 100

1 1 3 1 1 - 1 3 1 1 1

Dimethyl formamide
(DMF)

C3D7NO

100

1 1 4 1 1 4 3 - 1 3 1

Dimethyl sulfoxide
(DMSO)

C2H6SO

100

1 2 4 1 1 4 4 - 1 - 1

Dimethylaniline

C8H11N

100

- 3 4 2 4 - - - 1 - 1

Dioxane

C4H8O2

100

2 1 4 1 3 2 3 4 1 3 1

Dipropylene glycol
(mono)methyl ether

C4H10O

100

3 1 4 1 4 4 4 4 1 - 1

Ethyl acetate

C4H8O2

100

1 1 4 1 1 4 4 4 1 4 1

Ethylene chloride

C2H4Cl2

100

3 3 4 1 3 4 4 4 1 - 1

Ferrous chloride

FeCl2

saturated

1 3 1 3 1 1 1 1 1 - 4

Formaldehyde solution

CH2O

30

1 3 1 1 1 - - - 1 2 1

Formic acid

CH2O2

100

1 4 3 4 1 3 3 1 1 2 1

Furfural

C5H4O2

100

1 3 3 2 4 - - - 1 4 1

Gasoline

C5H12 - C12H26

100

2 1 3 1 3 3 2 - 1 1 1

Glycerol

C3H8O3

100

1 1 3 1 1 1 1 2 1 1 1

Heptane, normal

C7H16

100

2 1 1 1 2 1 2 4 1 1 1

Hexane, n-

C6H14

100

2 1 2 1 2 1 2 4 1 1 1

Hydrogen chloride

HCl 5 1 4 1 4 1 1 1 - 1 2 4

Hydrogen chloride

HCl

concentrated

1 4 4 4 1 1 2 3 1 4 4

Hydrogen peroxide

H2O2 3 1 3 1 1 1 1 1 - 1 3 3

Hydrogen peroxide

H2O2

30

1 4 1 4 1 1 1 - 1 3 3

Hydrogen sulphide

H2S

10

1 1 1 1 1 1 1 3 1 3 1

Iodine, tincture of

I2 1 4 3 1 1 - 4 4 1 1 1

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

63 / 72

Translation from the original operating manual, part no. 0700602

- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, weak

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Isopropyl alcohol

C3H8O

100

1 1 1 1 1 1 1 4 1 - 2

Lactic acid

C3H6O3

3

1 3 1 2 1 1 2 - 1 1 1

Magnesium chloride

MgCl2

10

1 1 1 1 1 1 1 1 1 1 1

Mercuric chloride

HgCl2

10

1 4 1 3 1 1 1 1 1 1 4

Mercury

Hg

100

1 1 1 1 1 1 1 3 1 1 3

Methyl acetate

C3H6O2

100

1 1 4 2 1 - 4 4 1 - 1

Methyl alcohol

CH4O

100

1 2 4 1 1 3 1 3 1 2 1

Methyl benzene

C7H8

100

3 1 4 1 3 4 4 4 1 4 1

Methyl ethyl ketone
(MEK)

C4H8O

100

1 1 4 1 1 4 4 4 1 4 1

Methylene chloride

CH2Cl2

100

4 3 4 3 3 4 4 4 1 - 1

Mineral oil — 100

1 1 1 1 1 1 1 - 1 1 1

Nitric acid

HNO3

10

1 4 1 4 1 1 1 - 1 4 3

Nitric acid

HNO3

100

4 4 4 4 4 - 4 - 1 4 1

Nitrobenzene

C6H5NO2

100

3 4 4 3 2 4 4 4 1 4 1

Oleic acid

C18H34O2

100

1 1 1 2 1 - 1 - 1 3 1

Oxalic acid

C2H2O4 x 2H2O

100

1 3 1 4 1 1 1 1 1 2 1

Ozone

O3

100

3 4 1 4 3 1 1 - 1 4 2

Petroleum — 100

1 1 3 1 1 1 1 3 1 1 1

Phenol

C6H6O

10

1 4 4 4 1 4 1 3 1 3 1

Phenol

C6H6O

100

2 4 4 4 1 3 4 3 1 3 1

Phosphoric acid

H3PO4

20

1 4 2 4 1 - - - 1 2 4

Phosphorus
pentachloride

PCl5

100

- 4 4 4 1 - 4 4 1 - 1

Potassium hydrogen
carbonate

CHKO3

saturated

1 1 2 1 1 - - - 1 - 4

Potassium hydroxide

KOH

30

1 1 4 3 1 1 1 1 1 - 4

Potassium hydroxide

KOH

50

1 1 4 3 1 1 1 1 1 - 4

Potassium nitrate

KNO3

10

1 1 1 1 1 - - - 1 1 1

Potassium
permanganate

KMnO4

100

1 4 1 1 1 - 1 - 1 3 1

Pyridine

C5H5N

100

1 1 4 1 3 4 4 4 1 4 1

Resorcinol

C6H6O2

5

1 4 2 3 1 4 2 - 1 - 2

Silver nitrate

AgNO3

100

1 1 1 1 1 1 1 1 1 2 4

Laboratory Centrifuge Sigma 2-16P

11 Appendix

64 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, weak

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Sodium bisulphite

NaHSO3

10

1 1 2 4 1 - - - 1 1 1

Sodium carbonate

Na2CO3

10

1 1 1 1 1 - - - 1 - 3

Sodium chloride

NaCl

30

1 1 1 1 1 1 1 1 1 1 3

Sodium hydroxide

NaOH

30

1 1 4 1 1 1 1 1 1 2 4

Sodium hydroxide

NaOH

50

1 1 4 1 1 1 1 - 1 2 4

Sodium sulfate

Na2SO4

10

1 1 1 1 1 1 1 1 1 1 1

Spirits

C2H6O

96

1 1 1 1 1 1 1 3 1 - 1

Styrene

C8H8

100

4 1 4 1 3 - 4 4 1 4 1

Sulphuric acid

H2SO4 6 1 4 1 4 1 1 1 - 1 2 3

Sulphuric acid

H2SO4

fuming

4 4 4 4 4 4 4 4 1 4 3

Tallow — 100

1 1 1 1 1 - 1 1 1 1 1

Tetrahydrofuran (THF)

C4H8O

100

3 1 4 1 3 4 4 4 1 3 1

Tetrahydronaphthalene

C10H12

100

3 1 4 1 4 4 4 4 1 - 1

Thionyl chloride

Cl2SO

100

4 4 4 2 4 4 4 4 1 - 3

Tin chloride

SnCl2

10

1 4 2 2 1 - - - 1 1 4

Transformer oil

—

100

1 1 3 3 1 1 1 - 1 1 1

Trichloroethane

C2H3Cl3

100

3 3 4 2 4 4 4 4 1 4 4

Urea

CH4N2O

10

1 1 1 1 1 - - - 1 1 1

Urine — 100

1 1 1 1 1 - 1 1 1 - 2

Vinegar

C2H4O2

10

1 4 1 1 1 1 1 1 1 2 1

Vinegar

C2H4O2

90

1 4 4 4 1 3 1 4 1 - 1

Wax — 100

- 1 1 1 - - - 1 - 1

Wines — 100

1 1 1 2 1 1 1 1 1 - 4

Xylene

C8H10

100

3 1 4 1 4 4 4 4 1 4 1

Pos: 217 /010 Universalmodule/Seiten wechsel @ 0\mod_1202116244312_0.d ocx @ 105 @ @ 1

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

65 / 72

Translation from the original operating manual, part no. 0700602

Pos: 218 /100 Sigma/110 BA Zentrifugen Sig ma (Projekte)/2-16P/110 Anhang/ 110-0060 Konformitätserklärung 2-16P @ 29\mod_1405321566376_68.docx @ 197113 @ 2 @ 1

11.5 EC declaration of conformity

Laboratory Centrifuge Sigma 2-16P

11 Appendix

66 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Laboratory Centrifuge Sigma 2-16P

11 Appendix

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

67 / 72

Translation from the original operating manual, part no. 0700602

11.6 Declaration of conformity – China RoHS 2

Laboratory Centrifuge Sigma 2-16P

11 Appendix

68 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

=== Ende der Liste für Textmarke Inhalt ===

Laboratory Centrifuge Sigma 2-16P

Index

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

69 / 72

Translation from the original operating manual, part no. 0700602

12 Index

A

Acceleration time

- see also the softstart and softstop function

............................................................ 37

Accessories, cleaning and care .................. 45

Acid................................................ 20, 44, 45

Activating the modification mode ................ 34

Adapters .............................................. 32, 45

Alkaline solutions ........................... 20, 44, 45

Ambient conditions ..................................... 53

Ambient temperature ................................. 53

Anodised coating ....................................... 45

Application examples ................................. 13

Autoclaving ................................................ 48

Auto-Lid-Open ........................................... 38

Automatic lid opening function ................... 38

Automatic rotor identification ...................... 37

B

Brief mains power failure ............................ 40

Bucket ........................................................ 32

Buckets, cleaning and care ........................ 46

Buzzer ....................................................... 38

C

Calling up stored programs ........................ 39

Capacity ..................................................... 53

Capillary tubes ........................................... 30

Carrier ........................................................ 32

Carrier systems .......................................... 32

Carriers, cleaning and care ........................ 46

CE mark in compliance with the directive

2006/42/EC ............................................ 15

Centrifugation of infectious, toxic, radioactive,

or pathogenic substances ....................... 19

Centrifugation principle .............................. 13

Centrifugation with different tube sizes ....... 31

Centrifugation with low capacity ................. 31

Centrifuge cannot be started ...................... 40

Centrifuge decelerates during operation .... 40

Centrifuge is inclined .................................. 40

Centrifuge was moved during run .............. 40

Centrifuge, cleaning and care .................... 44

Centrifuges

- definition ................................................. 9

Changes in colour ...................................... 48

Changes in structure .................................. 48

Changing the RCF increments ................... 36

Changing the speed increments ................. 36

Changing the time increments.................... 35

Chemical and biological safety ................... 19

Chemical resistance of plastic .................... 45

China RoHS 2 – Declaration of conformity . 67

Cleaning agents ......................................... 47

Cleaning the bores of angle rotors ............. 45

Cleaning the centrifuge .............................. 44

Closing the lid ................................ ...... 18, 27

Communication error ................................. 42

Condensation ............................................. 26

Condenser dirty ......................................... 40

Connected load .......................................... 53

Contamination ...................................... 19, 45

Continuous heat resistance ........................ 47

Continuous run .......................................... 35

Control system ........................................... 33

Copyright ................................................... 10

Corrosion ................ 18, 21, 32, 44, 45, 46, 47

Cost estimate ............................................. 50

Cracking .................................................... 45

Cracks ....................................................... 46

Current consumption .................................. 53

Customer-provided fuses ........................... 26

D

Damage of the surface ............................... 46

Dangerous goods....................................... 20

Dangerous materials .................................. 47

Deceleration time

- see also the softstart and softstop function

............................................................ 37

Declaration of conformity ..................... 10, 65

Declaration of conformity – China RoHS 2 . 67

Declaration of decontamination .................. 50

Declaration of Decontamination ................. 52

Decontamination agent ........................ 45, 47

Deformation of tubes .................................. 48

Laboratory Centrifuge Sigma 2-16P

Index

70 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Density ................................................. 12, 20

Different service life of rotors and accessories

......................................................... 21, 60

Dimensions ................................................ 24

Direct hazard to the life and health ............. 16

Directive 2002/96/EC ................................. 52

Disinfectants .............................................. 47

Disinfection of the rotor chamber and

accessories ................................ ............ 47

Display ....................................................... 33

Disposal of the centrifuge ........................... 52

Disposal of the packaging .......................... 52

Documentation ........................................... 10

Drive problem ............................................ 40

E

Earth conductor check ............................... 22

EC declaration of conformity ................ 10, 65

EEPROM error ........................................... 42

Electrical connection .................................. 53

Electrical safety .......................................... 18

Emergency lid release ............................... 41

Equipotential bonding screw ...................... 22

Error correction .......................................... 40

Error message ........................................... 40

Explanation of the symbols and notes ........ 16

Explosive substances ................................ 19

F

Fire preventions ......................................... 19

Form for the return of defective parts ......... 50

Functional and operating elements ............ 11

Fuses have tripped .................................... 40

G

General conditions ................................ ....... 9

Glass breakage .......................................... 47

Glass particles ........................................... 47

Gravitational field ....................................... 53

Grease for load bearing bolts ..................... 44

H

Hazard warnings ........................ 9, 28, 29, 32

Highly corrosive substances ...................... 18

I

Imbalance ...................................... 31, 32, 46

Imbalance error .......................................... 42

Imbalance monitoring system .................... 22

Importance of the operating manual ............. 9

Important information ................................. 16

Improper loading ........................................ 40

Infectious substances ................................ 47

Inflammable substances ............................ 19

Informal safety instructions ........................ 17

Initial start-up ................................ ............. 27

Input fuse ............................................. 12, 53

Input lock ................................................... 38

Inspection by the manufacturer .................. 49

Installation of accessories .......................... 31

Installation of angle rotors with a hermetically

sealed lid ................................................ 29

Installation of rotors and accessories ......... 28

Installation of the microhaematocrit rotor ... 30

Installation of the rotor ............................... 28

Installation site ........................................... 26

Intended use ................................................ 9

Interrupting a centrifugation run ................. 34

Interrupting a deceleration process ............ 34

IP code ...................................................... 53

K

kin. energy ................................................. 53

Kinetic energy ............................................ 12

L

Layout of the centrifuge.............................. 11

Leaks ................................................... 32, 48

Lid cannot be opened ................................ 40

Lid error ..................................................... 42

Lid lock device ........................................... 22

Lid lock has not released ........................... 40

Lid seal sticks ............................................ 40

Load bearing bolts, cleaning and care ........ 46

M

Mains power switch off ............................... 40

Mains switch .............................................. 11

Mains voltage ............................................. 18

Maintenance .............................................. 44

Malfunctions and error correction ............... 40

Manual mode ............................................. 34

Manufacturer .............................................. 53

Marking of the unit ..................................... 15

Maximum speed for tubes .......................... 57

Laboratory Centrifuge Sigma 2-16P

Index

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

71 / 72

Translation from the original operating manual, part no. 0700602

Measures in the event of hazards and

accidents ................................................ 23

Mechanical safety ...................................... 18

Microhaematocrit rotor, cleaning and care . 46

Mode of operation ...................................... 13

Motor error ................................ ................. 42

Motor shaft ............................... 25, 28, 29, 44

Multiple carrier ........................................... 32

N

Name plate .......................................... 11, 12

No indication on the display ....................... 40

No power in the mains supply .................... 40

Noise level ................................................. 53

Nominal voltage ......................................... 12

Non-greased bolts ...................................... 46

Notes on safety and hazards ....................... 9

Notes on transport ..................................... 24

O

Online download of forms .......................... 51

Opening the lid ........................................... 27

Operating personnel .................................. 17

Operating voltage....................................... 26

Operational safety ...................................... 45

Overseas shipping ..................................... 24

P

Packaging .................................................. 25

Parameter error ......................................... 42

Pathogenic substances .................. 19, 44, 47

Plastic accessories, cleaning and care ....... 45

Potential hazard to the life and health ........ 16

Potentially hazardous situation................... 16

Power consumption ............................. 12, 53

Power cord is not plugged in ...................... 40

Power supply ............................................. 26

Pressure marks .......................................... 46

Prevention of accidents ................................ 9

Problem description ................................... 50

Process error ............................................. 42

Programs ................................................... 39

Protection class ................................... 26, 53

Q

Quitting the modification mode ................... 34

R

Radioactive substances ....................... 19, 44

Radius ....................................................... 14

Range of accessories ................................ 55

RCF ........................................................... 36

Relative centrifugal force (RCF) ........... 14, 36

Removal of the transport safety device ...... 25

Remove glass particles and metal dust from

the rotor chamber ................................... 47

Responsibility of the operator ..................... 17

Return of centrifuges, spare parts, or

accessories ............................................ 50

Return of defective parts ............................ 50

Risk of electrical shock............................... 18

Rotor chamber ........................................... 44

Rotor radii .................................................. 58

Rotor selection ........................................... 37

Rotor tie-down screw ..................... 28, 29, 46

Rotor wrench ................................. 10, 28, 29

Rotors and accessories with a different

service life ........................................ 21, 60

Rotors for microtiter plate formats .............. 28

Rotors, cleaning and care .......................... 46

Runtime ..................................................... 34

S

Safety area ................................................ 20

Safety devices ........................................... 22

Safety distance .............................. 18, 20, 26

Safety instructions ...................... 9, 28, 29, 32

Safety instructions for centrifugation .......... 20

Safety of rotors and accessories ................ 21

Safety, chemical and biological .................. 19

Safety, electrical ........................................ 18

Safety, mechanical ..................................... 18

Safety-conscious work ............................... 17

Saving the current settings ......................... 39

Scope of supply ......................................... 10

Selection, display, and modification of data 34

Serial number ................................ 12, 43, 49

Service ....................................................... 49

Service contact .......................................... 43

Service life ................................................. 44

Service life of rotors and accessories ... 21, 60

Service life of the accessories .................... 48

Service work .............................................. 49

Laboratory Centrifuge Sigma 2-16P

Index

72 / 72

Version 04/2007, Rev. 2.15 of 15/09/2017 • sb

Translation from the original operating manual, part no. 0700602

Set-up and connection ............................... 26

Short run .................................................... 35

Softstart and softstop function .................... 37

Solvents ......................................... 20, 44, 45

Sound signal (buzzer) ................................ 38

Spare part enquiries ................................... 43

Speed ...................................... 12, 14, 36, 53

Speed-gravitational-field-diagram .............. 59

Speedometer error ..................................... 42

Standards and regulations ......................... 10

Standstill monitoring system ...................... 22

Starting a centrifugation run ....................... 34

Sterilisation of the rotor chamber and

accessories ................................ ............ 47

Storage and transport ................................ 24

Storage conditions ..................................... 24

Storage locations ....................................... 53

Stress-corrosion

see

corrosion ............................................. 46

Structural changes ................................ ..... 19

Supply voltage ........................................... 26

Switching the centrifuge off ........................ 39

Switching the centrifuge on ........................ 27

System check ............................................ 22

System error ........................................ 40, 42

T

Table of error codes ................................... 42

Table of rotors and accessories with a

different service life .......................... 21, 60

Technical data ........................................... 53

Technical documentation ........................... 54

Temperature error ...................................... 42

Temperature value not reached ................. 40

Thermal stress ........................................... 44

Toxic substances ................................. 19, 44

Transport safety device .............................. 25

Tubes ......................................................... 32

Type........................................................... 12

Type of connection ..................................... 26

Type of the centrifuge .................... 43, 49, 53

U

Ungreased load- bearing bolts ................... 40

Units of protection class I ........................... 26

Useful volume

- volume that is stated for the tube .... 32, 57

User interface ............................................ 33

UV radiation ......................................... 26, 44

V

Vents ......................................................... 26

Vessels ...................................................... 32

W

Warranty and liability .................................... 9

Wear .......................................................... 49

Weight ....................................................... 24

Work on the power supply system ............. 18

Y

Year of manufacture .................................. 12